18 results about "Penicillamine" patented technology

The invention discloses a chiral amino acid CPL probe based on an aldehyde group functionalized luminescent rare earth spirochete complex as well as a preparation method and application thereof, and belongs to the technical field of biosensing materials. The invention aims to solve the problem that the existing probe has poor selectivity in recognition of sulfydryl amino acid. The chiral amino acid CPL probe is an aldehyde group functionalized water-soluble lanthanide helical complex, side chain aldehyde groups can enhance the water solubility of the helical complex by forming hemiacetal and can also be used as specific recognition sites, the aldehyde groups react with sulfydryl-containing amino acids such as D-penicillamine and L-cysteine to form thiazolidine rings, and the chiral amino acid CPL probe can be used as a specific recognition site. Furthermore, the chirality of D-PA and L-Cys is triggered to be transmitted to a metal center, and the chirality is amplified into a detectable CPL signal. Thiazolidine rings formed by condensation of different amino acids have steric hindrance differences, and the steric hindrance differences can influence spiral chirality, so that the probe still shows excellent selectivity even in a complex system in which a plurality of interfering substances coexist.

This invention relates to the field of fluorescence detection technology, specifically disclosing a dual-colorimetric fluorescent probe for detecting methyl orange, its preparation method, and its application. The probe is prepared by in-situ synthesis of carbon dots and copper nanoclusters. The carbon dots are synthesized from Bermuda grass as a precursor via a hydrothermal method, and the copper nanoclusters are obtained by reducing copper ions with penicillamine and bovine serum albumin as dual stabilizers. The dual-colorimetric fluorescent probe provided by this invention has a detection limit for methyl orange as low as 0.06 μM, exhibiting good selectivity, stability, and anti-interference ability. It can be applied to the spiked recovery detection of methyl orange in actual water samples and can achieve visualized semi-quantitative analysis through filter paper loading, making it suitable for rapid on-site screening.

The invention discloses preparation and application of a nano enzyme. The preparation method of the nano enzyme comprises the following steps: dissolving copper salt and hydroxy anthraquinone carboxylic acid in water to obtain a first solution; adjusting the pH of the first solution to be alkaline, and heating for reaction to obtain a product solution; and centrifuging the product solution, taking the precipitate, washing and drying to obtain the Cu nano-enzyme. The copper salt comprises at least one of copper sulfate, copper chloride, copper nitrate and copper hydroxide; the hydroxy anthraquinone carboxylic acid is rhein. According to the invention, Cu is used as a transition metal source and reacts with specific hydroxy anthraquinone carboxylic acid to prepare the Cu nano-enzyme (Cu-Rhein) with excellent peroxidase-like activity. The Cu nano enzyme can be applied to promotion of hydrogen peroxide oxidation TMB color development and detection of penicillamine, the accuracy of penicillamine detection is high, and the Cu nano enzyme has good application prospects.

The invention belongs to the technical field of biological medicine, and particularly relates to a nanometer drug delivery system with a tumor deep penetration effect and a preparation method and application of the nanometer drug delivery system. The polylactic acid-glycolic acid nano-particles are polylactic acid-glycolic acid copolymer nano-particles entrapped with chemotherapeutic drugs and carbon monoxide donors; the chemotherapeutic drug is monomethyl aurestatin E, and the carbon monoxide donor is S-nitroso-N-acetyl-DL-penicillamine; a lipid layer; the core is coated with the core; the targeting polypeptide is modified on the surface of the lipid layer; the targeting polypeptide is an R4F polypeptide; tumor cells are actively targeted through R4F polypeptide, the polylactic acid-glycolic acid copolymer nanoparticles wrapped by a lipid layer deeply penetrate into tumor tissues, nitric oxide is released through a carbon monoxide donor to degrade an extracellular matrix barrier, and deep penetration of loaded chemotherapeutic drugs and immune cells in the tumor tissues is synergistically promoted; the nano drug delivery system provided by the invention can be applied to preparation of drugs for treating solid tumors and delivery of drugs for targeting extracellular matrixes of the solid tumors.

The application discloses a preparation method of high-purity Fmoc-S-trityl-L-penicillamine and belongs to the field of synthetic chemistry. The method is characterized in that L-penicillamine is used as a starting material, dehydrated with methyl boronic acid to generate a cyclic protective structure, then a thiol group is selectively protected by trityl, and an acid is used to open a ring to obtain an S-trityl-L-penicillamine intermediate; then, in a sodium bicarbonate buffered water / dioxane weak alkaline system, Fmoc-OSu (9-fluorenylmethoxycarbonyl succinimidyl ester) is used to react with the S-trityl-L-penicillamine intermediate to protect an amino group, and racemization is effectively inhibited; finally, the product is refined by recrystallization in an ethyl acetate / n-heptane system to obtain a high-purity end product. The process route is simple and efficient, the conditions are mild, the purification method is unique and effective, the obtained product has high chemical purity and optical purity, good stability, is suitable for large-scale production, and can meet the needs of high-end polypeptide drug synthesis.

The application belongs to the technical field of carbon nanomaterial application, and particularly relates to chiral carbon nanomaterial for preventing and treating tobacco mosaic virus and a preparation method and application thereof. The application mainly solves the problem of poor virus prevention and treatment effect caused by easy inactivation and inability to continuously act of carbon nanomaterial in the existing method for preventing and treating plant virus based on carbon nanomaterial. The application relates to D-penicillamine modified carbon dots, which can destroy the protein shell of tobacco mosaic virus under right circular polarized light irradiation, inactivate the tobacco mosaic virus, and thus lose the infectivity, so as to achieve the purpose of preventing and treating tobacco mosaic virus. The application has the advantages of high stability, sustainable action and excellent virus prevention and treatment effect.

This invention discloses a chiral CuO / HgS heterojunction catalyst, its preparation method, and its application, belonging to the field of inorganic chiral materials technology. The chiral CuO / HgS heterojunction includes D-CuO / D-HgS heterojunction, L-CuO / L-HgS heterojunction, D-CuO / L-HgS heterojunction, and L-CuO / D-HgS heterojunction. Its preparation method includes: (1) preparing chiral CuO powder using a liquid-phase synthesis method with chiral cysteine ​​and CuCl2; (2) obtaining chiral HgS crystal nuclei by reacting Hg(NO3)2 solution with chiral penicillamine and thioacetamide, and dispersing them in water to obtain chiral HgS seed colloids; (3) synthesizing chiral CuO / HgS heterojunctions using a seed-mediated epitaxial growth method. Using the prepared chiral material for photocatalytic CO2 reduction can yield high-value-added C2 and C3 products, achieving efficient energy utilization.

The invention provides a kit for detecting homocysteine and metabolites thereof in serum. The kit comprises a calibrator and a sample releasing agent, the calibrator comprises a component 1 and a component 2; the component 1 comprises a BSA (Bovine Serum Albumin) matrix and a first protective agent; the component 2 comprises homocysteine, metabolite of the homocysteine and a second protective agent; each of the first protective agent and the second protective agent is prepared from ascorbic acid, dithiothreitol, N-acetyl-DL-penicillamine and EDTA (Ethylene Diamine Tetraacetic Acid). According to the present invention, the 5-methyltetrahydrofolic acid and the homocysteine which are easily oxidized are well protected, and the synchronous detection of the folic acid, the 5-methyltetrahydrofolic acid, the vitamin B2, the pyridoxal phosphate, the methylmalonic acid, the homocysteine and the methionine is achieved. The kit has good stability at 4 DEG C and can meet the use requirement.

This invention relates to the field of nanomaterials technology and discloses a chiral Cu 2‑x S-quantum dots, their preparation methods, and applications: In an aqueous phase, a copper source and a complexing agent are mixed and reacted to obtain a copper complex solution. A sulfur source and a dextrorotatory penicillamine chiral ligand are added to the copper complex solution. The mixed reaction solution is heated to carry out nucleation and growth reactions. After centrifugation and drying of the precipitate, chiral Cu with high photothermal conversion efficiency is obtained. 2‑x S-quantum dots. This invention is the first to use dextrorotatory penicillamine, which has a significant steric hindrance effect, as a chiral ligand during the growth of quantum dots. Its unique molecular structure induces the formation of a specific surface coordination environment and chiral structure. This structure can significantly enhance the absorption of near-infrared light by quantum dots and promote the non-radiative relaxation conversion of light energy into heat energy, greatly improving photothermal conversion performance. This provides an efficient and feasible technical solution for the practical application of chiral quantum dots in the field of tumor photothermal therapy.

The invention belongs to the field of biological medicines, and particularly relates to application of penicillamine in preparation of a medicine for preventing and treating noise-induced deafness. According to the specific technical scheme, the application of the penicillamine in preparing the medicine for preventing and treating hearing impairment / deafness is provided. The hearing disorder and / or deafness is caused by noise. It is found for the first time that penicillamine can play a hearing protection role by specifically regulating a cochlea immune microenvironment, and the hearing protection role is specifically achieved through the following mechanisms: inhibition of T cell activation: interference of a TCR signal channel; the hair cell structure is protected; the cochlea basilar membrane outer hair cell loss caused by noise exposure is reduced. Based on the new discovery of the invention, the penicillamine can be used for preventing and treating hearing impairment and deafness caused by noise.

The invention relates to the technical field of biomedical detection, in particular to a plasma detection device for detecting concentration of penicillamine, which comprises a bottom support, a test tube support, a detection processing mechanism and a needle cleaning mechanism are arranged at the top of the bottom support, an upper shell is arranged on the bottom support, and an automatic detection mechanism is arranged on the inner wall of the upper shell. A detection post-processing assembly is arranged on the automatic detection mechanism; the automatic detection mechanism is used for automatic detection, the needle head cleaning mechanism is used for cleaning the needle head after detection, manual participation is not needed in the whole process, the detection efficiency is improved, and the labor cost of detection is reduced; in the downward insertion process of the sampling needle head and the processing needle head, the air blowing purification mechanism is used for cleaning the space above the sealing diaphragm, so that dust, fibers and microbial aerosol are prevented from being brought into the packaging shell, and the concentration detection precision after the sampling needle head and the processing needle head are ensured.

The present application relates to a kind of magnesium oxide doped chiral cobalt-based nanomaterial and its preparation method and application, belong to nanomaterial technical field.The preparation method of the present application includes the following steps: S1, the cobalt source solution, magnesium source solution and surfactant solution are dissolved in organic solvent, to obtain precursor solution;S2, adding penicillamine solution to precursor solution, chiral environment is induced by coordination interaction, to obtain chiral intermediate solution;S3, adding reducing agent to chiral intermediate solution and carrying out self-assembly reaction, by centrifugation, washing, vacuum drying, to obtain magnesium oxide doped chiral cobalt-based nanomaterial;The catalytic activity of the obtained material is high, stability is good, and has wide application prospect in asymmetric photocatalytic reaction.

The application discloses a kind of alpha-amylase activator based on chiral quantum dots and preparation method and application, including the following steps: using chiral amino acid L / D-penicillamine as chiral source, modified graphdiyne quantum dots (GDQDs), the surface functionalization of GDQDs is prepared chiral graphdiyne quantum dots L (D) -GDQDs;Alpha-amylase is fully dissolved in buffer, and is fully mixed with L (D) -GDQDs to prepare alpha-amylase activator.The application provides a kind of new method for preparing activator to improve the activity of alpha-amylase, wherein L (D) -GDQDs used have good water solubility, stability and biological activity, can be combined with alpha-amylase to change the structure of enzyme, so as to affect the activity of enzyme, and has a significant effect on improving the catalytic activity of enzyme.

The application discloses a water-soluble achiral rare earth tetrahedral cage CPL probe for recognition of mercapto amino acids and a preparation method thereof, and relates to a CPL probe for recognition of mercapto amino acids and a preparation method thereof. The application is aimed at solving the problems that water solubility and functional cavities are difficult to balance, and specific recognition ability is limited in the detection of mercapto amino acids by using existing supramolecular cage optical probes. The water-soluble achiral rare earth tetrahedral cage CPL probe for recognition of mercapto amino acids has high water solubility, strong CPL signal and high selective rare earth tetrahedral cage probe, realizes efficient recognition and quantitative detection of mercapto amino acids such as D-penicillamine, and has the advantages that Eu4L4 is an achiral rare earth tetrahedral cage, the number of aldehyde groups is multiplied due to the tetrahedral structure, excellent circularly polarized luminescence performance is exhibited, high-precision distinction between mercapto amino acids and non-mercapto amino acids is successfully realized, and the selectivity of detection is remarkably improved.

The invention discloses a traditional Chinese medicine composition for treating scleroderma, which is prepared from the following traditional Chinese medicines in parts by weight: 60-100 parts of agkistrodon halys, 60-100 parts of cobra and 30-50 parts of dragon's blood. In the formula, the agkistrodon halys and the cobra have the effects of dispelling wind, dredging collaterals and resisting rheumatism, the dragon's blood has the effects of removing blood stasis and relieving pain, the formula conforms to the treatment principle of scleroderma for promoting blood circulation, removing blood stasis, dispelling wind and dredging collaterals, and research in recent years shows that the snake medicine still has the effect of regulating the immune function. Clinical experiment research shows that the treatment effect of the Soukang capsule taken by a scleroderma patient is compared with the treatment effect of a patient taking penicillamine in the same period, and the effectiveness and the safety of the Soukang capsule in treating systemic scleroderma are proved, so that the medicine disclosed by the invention has a remarkable effect of treating the scleroderma and has a good application value.

The invention relates to the technical field of composite materials, in particular to an MOF-based composite sensitizer as well as a preparation method and application thereof. According to the MOF-based composite sensitizer, Hf-TCPP is adopted as a core layer, hemoglobin and boron phenylalanine are loaded, pH-sensitive ZIF-8 is adopted as a core shell, S-nitroso-N-acetyl-DL-penicillamine is loaded, oxygen deficit is relieved, tumor cell energy metabolism is adjusted, and the BNCT curative effect is synergistically improved; a ZIF-8 shell layer is stable in a normal physiological environment, avoids early leakage of a boron carrier, is rapidly degraded in a tumor microenvironment, realizes precise controlled release of BPA and SNAP, is modified by hydrophilic polymers such as polyethylene glycol to prolong the blood circulation time and reduce immune clearance, and is functionally modified by folic acid. The preparation method disclosed by the invention is mild in reaction condition, integrates multiple functions of boron loading, targeted delivery, hypoxia relief, synergistic sensitization and the like, does not need to be additionally combined with other medicines, and simplifies a clinical treatment process.

The application provides a copper-penicillamine / imidazole nanometer enzyme with laccase-like activity and a preparation method and application thereof, and the preparation method comprises the following steps: S1, solution preparation: S1a, dissolving copper chloride in deionized water to obtain a copper chloride solution; S1b, dissolving penicillamine in deionized water to obtain a penicillamine solution; S1c, dissolving dimethyl imidazole in deionized water to obtain a dimethyl imidazole solution; S2, mixing the copper chloride solution, the penicillamine solution and the dimethyl imidazole solution with deionized water in a volume ratio of (15-2):(8-1):1:(18-2), and stirring at a temperature of 20-50 DEG C to obtain the copper-penicillamine / imidazole nanometer enzyme with laccase-like activity. The copper-penicillamine / imidazole nanometer enzyme with laccase-like activity prepared by the application can catalyze the degradation of various phenolic compounds, reduce the concentration of the phenolic compounds, and thus achieve the purpose of reducing the pollution of the phenolic compounds in water.

The invention provides a nitrosothiol modified vascular anastomosis device which comprises a tubular structure, a plurality of wire grooves are formed in the outer surface of the tubular structure, a nitric oxide release coating is loaded on the outer surface of the tubular structure, and the nitric oxide release coating is coated on the outer surface of the tubular structure. The nitric oxide release coating is a polydopamine coating, and S-nitroso-N-acetyl-DL-penicillamine is chemically grafted on the surface of the polydopamine coating. The invention further provides a preparation method of the nitrosothiol modified vascular anastomosis device. The preparation method comprises a design method of the vascular anastomosis device and a preparation method of the nitric oxide release coating. The nitrosothiol modified anastomosis device has the advantages that anastomosis is rapid, anastomosis strength is high, nitric oxide can be continuously released, growth of endothelial cells at the vascular anastomosis position is promoted, adhesion of platelets is inhibited, artery anastomosis operation in the main artery operation process can be effectively simplified, and the anastomosis effect is good. The endothelialization process of the blood vessel at the anastomotic stoma can be promoted, and the long-term patency rate of the blood vessel is increased.