27 results about "Trichloroacetic acid" patented technology

The invention belongs to the technical field of environmental engineering, and particularly relates to a rapid prediction method for trichloroacetic acid generation potential in a water disinfection process. The rapid prediction method for the trichloroacetic acid generation potential in the water disinfection process comprises the following steps: constructing a training set and a test set which respectively and independently comprise a plurality of organic compounds and trichloroacetic acid generation potential values and key molecular characteristic parameters corresponding to each organic compound; an extreme gradient lifting machine learning algorithm is adopted to construct a trichloroacetic acid generation potential prediction model, and model precision verification is carried out; optimizing by adopting a Bayesian optimization algorithm to obtain a trichloroacetic acid generation potential optimal prediction model; and inputting the key molecular characteristic parameters of the target compound into the optimal prediction model to obtain the trichloroacetic acid generation potential value of the target compound. The rapid prediction method provided by the invention realizes rapid prediction of the TCAAFP value of the organic compound in the chlorine disinfection reaction, and has the advantages of convenient operation, low cost, strong stability and wide application range.

This invention belongs to the field of recycling and purification technology, specifically relating to a method for recovering the β isomer from a CME solution containing both β and α isomers. The method includes the following steps: concentrating the CME solution containing both β and α isomers and dissolving it in methanol with trichloroacetic acid to form a salt, followed by cooling and crystallization and filtration to obtain the trichloroacetic acid salt of the CME β isomer; then, ionizing the trichloroacetic acid salt of the CME β isomer in methanol with ammonia to obtain the CME β isomer. By reacting trichloroacetic acid with the α and β isomers of CME to form a salt, the different solubilities of the trichloroacetic acid salts of the α and β isomers are utilized to separate the trichloroacetic acid salt of the CME β isomer, and then ionizing the trichloroacetic acid salt of the CME β isomer with ammonia, the CME β isomer is recovered.

The invention relates to a method for improving antioxidant activity of pine pollen polysaccharide by utilizing bifidobacterium fermentation, which comprises the following steps: S1, adding bifidobacterium liquid into pine pollen for fermentation to obtain a fermentation product; s2, adjusting the pH value of the fermentation product in the step S1 to be acidic, and adding protease for enzymolysis to obtain an enzymolysis product; s3, carrying out flash extraction on the enzymolysis product obtained in the step S2, and centrifuging to take supernate, so as to obtain a pollen pini crude polysaccharide extracting solution; and S4, adding a trichloroacetic acid solution into the pollen pini crude polysaccharide extracting solution obtained in the step S3, and dialyzing to obtain the pollen pini polysaccharide with improved antioxidant activity. Compared with the prior art, the pine pollen polysaccharide obtained through fermentation of bifidobacteria can obviously improve the antioxidant activity, improve the development and utilization value of pine pollen and improve the application and market competitiveness of the pine pollen polysaccharide in multiple fields compared with the pine pollen polysaccharide obtained through unfermented pine pollen polysaccharide.

The application relates to the technical field of biological detection, and particularly relates to a sample processing liquid and application thereof in ELISA detection of milk and milk products. The sample processing liquid comprises the following components in percentage by weight: 2-3% of ethylenediaminetetraacetic acid disodium salt dihydrate; 0.075-0.125% of trichloroacetic acid; 10-30% of methanol; and the balance is ultrapure water; and the sample is milk and milk products. The sample processing liquid provided by the application can efficiently and conveniently reduce the matrix interference of milk and milk products in ELISA detection, so that the accuracy, precision and recovery rate of detection can be significantly improved.

The invention provides a method for simultaneously detecting dichloroacetic acid and trichloroacetic acid in urine, and belongs to the technical field of analysis detection and occupational health / environmental health. According to the method disclosed by the invention, a synergistic pretreatment strategy of acidic sample loading, WAX / reversed-phase mixed mode solid-phase extraction, water / low-proportion organic phase step-by-step leaching and ammonia-water-containing organic phase elution is adopted; a technical route of protein precipitation, acidification pH regulation, purification and enrichment by adopting WAX / reversed-phase mixed mode solid-phase extraction on a 96-well plate and stable isotope internal standard isotope dilution LC-MS / MS quantification is adopted, the method can effectively reduce the matrix effect and realize high-throughput analysis in a high-salinity urine matrix, the quantitation limits of DCEA and TCAA are not higher than 0.30 g / L and 0.20 g / L respectively, and the method has the advantages that the method is simple and convenient to operate, and the method is suitable for large-scale popularization and application. The method is suitable for biological monitoring and risk assessment of occupational and environmental low-level exposure crowds.

The invention relates to the technical field of food detection, discloses an efficient detection method for six sugar alcohol compounds in dairy products, and aims to solve the problems that in the prior art, dairy product protein precipitation and high-efficiency dissolution of strong-polarity sugar alcohol are difficult to consider at the same time, and toxic precipitants, high-temperature treatment or complex solid-phase extraction are often depended on. The problems of tedious operation, high equipment threshold and poor detection stability exist in the prior art, trichloroacetic acid with optimized concentration is used for constructing a water phase system, and dissolution of six sugar alcohols and preliminary precipitation of protein are synchronously realized; the high-protein matrix of the milk powder is supplemented with precise supplementary precipitation, and the liquid milk is simplified; a specific chromatographic column and gradient elution are combined, linear correlation is established by adopting an external standard method, synchronous detection of six sugar alcohols is completed, high-temperature and solid-phase extraction consumables are not needed, two matrixes of milk powder and liquid milk are adapted, the operation time is short, the detection recovery rate is high, the precision is excellent, the existing pain point is effectively solved, and the batch detection requirement of a conventional laboratory is met.

The invention relates to the technical field of biological detection, in particular to sample treatment liquid and application thereof in ELISA (enzyme-linked immunosorbent assay) detection of milk and milk products. The sample treating fluid comprises the following components in percentage by weight: 2-3% of disodium ethylene diamine tetraacetate dihydrate; 0.075 to 0.125% of trichloroacetic acid; 10 to 30% of methanol; and the balance of ultrapure water. The sample is milk and a milk product. The sample treatment liquid provided by the invention can efficiently and conveniently reduce matrix interference of milk and milk products in ELISA detection, so that the detection accuracy, precision and recovery rate can be remarkably improved.

Topical formulations comprising peroxyacids prepared in situ from trichloroacetic acid, dichloroacetic acid, monochloroacetic acid or acetic acid and hydrogen peroxide are useful for promoting hair growth and for preventing hair loss.

The present invention addresses the problem of providing an albumen hydrolysate that has emulsifiability, emulsion stability and heat coagulability. An albumen hydrolysate obtained by hydrolysis of albumen with a protease, wherein the dry weight of a precipitate formed by adding nine times the amount of 0.4 M trichloroacetic acid (TCA) to the albumen hydrolysate is 60% or more relative to the dry weight of albumen treated in the same manner.

Disclosed is a detergent composition, comprising the following reagent and reagent combination: a first reagent, i.e., a potassium salt or a sodium salt; and a second reagent combination, i.e., trichloroacetic acid, acetonitrile, cyclodextrin, or a cyclodextrin derivative. The detergent composition in the present disclosure can be used to remove a surfactant used in a protein extraction process.

The invention relates to a SiO2-coated Al2O3 loaded rare earth three-way catalyst, a preparation method thereof and application of the catalyst in polycarbonate synthesis, and belongs to the technical field of polymer synthesis. According to the catalyst, SiO2-coated Al2O3 serves as a carrier to load a rare earth three-way catalyst, SiO2-coated Al2O3 is of a double-layer structure, the inner layer of SiO2-coated Al2O3 is SiO2, the outer layer of SiO2-coated Al2O3 is Al2O3, and the rare earth three-way catalyst contains yttrium trichloroacetate, glycerin and diethyl zinc. The SiO2-coated Al2O3 carrier with a double-layer structure is prepared, and the rare earth three-way catalyst is loaded by utilizing the characteristics of large specific surface area, abundant radial pore structures and abundant contactable active surfaces of the SiO2-coated Al2O3 carrier, so that the efficient polycarbonate synthesis catalyst is obtained; the catalyst has the characteristics of high catalytic efficiency (TON), high polycarbonate molecular weight, high carbonate chain link content, easiness in separation after the reaction is finished, and the like.

Pharmaceutical, dental, cosmetic and dermatological compositions comprising an addition salt of trichloroacetic acid, one or more hydroxy acids and optionally glutamic acid or bioisosteres of glutamic acid and phytic acid are useful for peeling of the gum collar, exfoliating the skin, resurfacing, treating skin hyperpigmentation, controlling sebum production, acne, reducing pore size and reducing the appearance of scars and uneven skin tone without causing undesirable side effects, for fibroblast stimulation, transdermal biorevitalization, stimulation of new collagen production, for aesthetic improvement, skin whitening, skin brightening, skin tightening and skin rejuvenation.

The invention discloses a method for preparing short-chain diacid by degrading polyethylene with flue gas. The method comprises the following steps: mixing an ionic liquid and polyethylene, and reacting in a flue gas and air environment to obtain the short-chain diacid; the ionic liquid comprises [trihexyltetradecyl phosphorus] [benzenesulfonic acid], [trihexyltetradecyl phosphorus] [trifluoroacetic acid], [trihexyltetradecyl phosphorus] [acetic acid], [trihexyltetradecyl phosphorus] [trichloroacetic acid] and the like. The method not only can realize polyethylene degradation and flue gas denitration treatment, but also can realize efficient preparation of short-chain diacid, the concentration of NOx in the tail gas after reaction is lower than 50ppm, the process is green and pollution-free, the operation is simple, and the concept of green chemistry is practiced from multiple angles.

This invention discloses a method for the electrocatalytic hydrogenation dehalogenation of haloacetic acids or halomethanes in water. A conductive material surface-modified with ruthenium or platinum is used as the catalytic cathode, and water is used as the hydrogen source to electrochemically hydrogenate and dehalogenate haloacetic acids or halomethanes in water into acetic acid or methane. The precious metals in the catalytic cathode of this invention are cheaper; the unit price of ruthenium is much lower than that of palladium and rhodium. Compared with palladium-modified catalytic cathodes, the dechlorination selectivity is higher, with an 11.7% increase in the selectivity for the hydrogenation and dechlorination of trichloroacetic acid in water to acetic acid.

The present application relates to the technical field of determination of nifursol metabolite content, and particularly relates to a method for determining the residual amount of nifursol metabolite in royal jelly. The method comprises the following steps: the royal jelly sample is sequentially subjected to trichloroacetic acid hydrolysis, o-nitrobenzaldehyde derivatization and ethyl acetate purification, and is treated with acetonitrile aqueous solution to constant volume, the pretreated sample is subjected to gradient elution using 5 mmol / L ammonium acetate aqueous solution-acetonitrile as a mobile phase, and is determined by liquid chromatography-tandem mass spectrometry, and the isotopic internal standard method is used for qualitative and quantitative analysis during determination. The royal jelly sample is hydrolyzed using a trichloroacetic acid solution before derivatization treatment, so that the to-be-measured component is well separated from the sample matrix, the matrix influence is reduced, and then the liquid chromatography-tandem mass spectrometry analysis is performed in combination with the selection of the mobile phase and the isotopic internal standard method, thereby improving the qualitative and quantitative accuracy of the residual amount of nifursol metabolite in royal jelly.

The invention discloses a method for detecting related substances of chloral hydrate and a preparation thereof, which adopts a high performance liquid chromatography, and the chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a chromatographic column of a filler, an acid-containing aqueous solution is used as a mobile phase A, acetonitrile is used as a mobile phase B, and gradient elution comprises 90-97% of the mobile phase A in 0-5min; the mobile phase A is 80-90% in 9-12 min; the mobile phase A accounts for 10-30% in 25-35 min; according to the related substance detection method, degradation impurities such as formic acid, acetic acid and trichloroacetic acid and process impurities such as 2, 2, 3, 3-tetrachlorosuccinic acid can be detected at the same time by optimizing the elution gradient, and the detection method is high in sensitivity, accuracy and precision and good in reproducibility.

Provided is the large-scale preparation of a high-purity hydrolyzed colanic acid or a salt thereof. The method comprises: 1) adding a solution containing a colanic acid or a salt thereof to a colanic acid degradation enzyme comprising an amino acid sequence as shown in SEQ ID NO: 1 or having at least 90% sequence identity thereto; and 2) performing an enzyme termination reaction, wherein the termination in step 2) is achieved by means of a termination agent, a boiling water bath and / or an ice bath, wherein the termination agent is selected from one or more of activated carbon, absolute ethanol, methanol, EDTA, trichloroacetic acid and sodium carbonate, and preferably, activated carbon is used to achieve the termination in step 2), so as to obtain the hydrolyzed colanic acid or a salt thereof. The molecular structure of the hydrolyzed sodium colanate prepared by the provided method features acetylation and pyruvic acid modification. Moreover, the preparation conditions are mild, the production cost is low, continuous production can be achieved, and industrial scale-up is easily implemented. The prepared hydrolyzed sodium colanate has the characteristics of high purity and low endotoxin residue, and has potential and broad application value in fields such as cosmetics, medical devices, and oligosaccharide drugs.

This invention discloses a biomimetic-microwave-assisted alkaline extraction combined with ultrasonic extraction method for extracting acidic pine polysaccharides, belonging to the field of polysaccharide technology. The method includes the following steps: Step 1, enzymatic hydrolysis; Step 2, microwave-assisted alkaline extraction; Step 3, ultrasonic-assisted extraction; Step 4, combining the extracts to obtain a concentrated solution, precipitating with alcohol overnight, centrifuging, discarding the supernatant, redissolving the precipitate in deionized water, adding trichloroacetic acid (TCA), stirring, incubating overnight at 0-4°C, centrifuging, dialyzing the supernatant, and then freeze-drying to obtain acidic pine polysaccharides. This biomimetic-microwave-assisted alkaline extraction combined with ultrasonic extraction method for extracting acidic pine polysaccharides significantly improves the polysaccharide yield, is environmentally friendly, enhances the immunomodulatory activity of the polysaccharides, and solves various problems in traditional extraction methods.

The invention belongs to the field of surfactant removal, particularly relates to a washing composition and application thereof, and more particularly relates to a washing composition for removing a surfactant and application thereof. The invention provides a washing composition. The washing composition comprises the following reagents and reagent combinations: a first reagent: potassium salt or sodium salt; and a second reagent combination: trichloroacetic acid, acetonitrile, cyclodextrin or a cyclodextrin derivative. By using the washing composition disclosed by the invention, the surfactant used in the protein extraction process can be furthest removed through the synergistic interaction between the reagents and the reagent combination, and meanwhile, the loss of low-abundance protein and difficulty in redissolving the protein in the preparation process of other methods are avoided.

This invention belongs to the field of pathological testing and tissue specimen preservation technology, and in particular, it is a compound solvent-based tissue fixative, which is made by mixing the following raw materials in weight percentages: the main solvent is a 60%-80% ethanol solution; the co-solvent and penetrant is 5%-10% polyethylene glycol tetrahydric acid and 3%-8% diethylene glycol; the dehydrating and hardening regulator is 5%-10% acetone; the pH adjuster and protein precipitant is a 2%-5% glacial acetic acid and 1%-3% trichloroacetic acid solution; the antibacterial and antistatic agent is a 0.5%-2% quaternary ammonium salt solution, wherein the quaternary ammonium salt solution is a 1% octyldecyl dimethyl ammonium chloride solution; and the stabilizer is 1%-3% methoxy-3-methylbutanol. This invention significantly reduces the health risks to operators by eliminating toxic reagents such as formaldehyde and glutaraldehyde and using food-grade ethanol as the main solvent, thus meeting green and environmentally friendly requirements. This characteristic enables the fixative to provide safer working conditions in environments such as pathology laboratories where long-term contact with fixatives is required, thus protecting the health of operators.

The invention provides a method for measuring the concentration of a < 125 > I labeled drug in whole blood, which comprises the following steps: adding a trichloroacetic acid solution with the concentration of 250 g / L into a collected whole blood sample, uniformly mixing, centrifuging, removing supernate, and collecting a first precipitate; adding a trichloroacetic acid solution with the concentration of 100-250 g / L into the first precipitate, uniformly mixing, centrifuging, removing supernate, and collecting a second precipitate; adding trichloroacetic acid solutions with the concentration of 100-250 g / L into the second precipitate, uniformly mixing, centrifuging, removing supernate, collecting a third precipitate, and detecting the radioactivity count of the third precipitate by adopting a gamma-counter. The method provided by the invention can be used for removing free < 125 > I generated by deiodination and free < 125 > I adsorbed on the surface of a precipitate, so that errors caused by inaccurate detection concentration due to deiodination are avoided, the operation is simple and convenient, the sensitivity is high, and the test pretreatment and detection efficiency and accuracy are greatly improved.

The present application belongs to the field of biotechnology, and relates to a method for large-scale preparation of high-purity hydrolyzed colanic acid or a salt thereof. The method comprises: 1) adding a solution containing colanic acid or a salt thereof into a colanic acid-degrading enzyme comprising an amino acid sequence as shown in SEQ ID NO: 1 or having at least 90% sequence identity thereto; and 2) enzyme termination reaction: termination in step 2) is achieved by a termination agent, a boiling water bath and / or an ice bath, wherein the termination agent is selected from one or more of activated carbon, anhydrous ethanol, methanol, EDTA, trichloroacetic acid and sodium carbonate, preferably termination in step 2) is achieved by using activated carbon; thereby obtaining hydrolyzed colanic acid or a salt thereof. The hydrolyzed sodium colanic acid prepared by the method provided in the present application has a molecular structure with acetylation and pyruvic acid modification, and the preparation conditions are mild, the production cost is low, continuous production can be realized, and industrial amplification is easy.

The application provides a metal-modified ZSM-5 molecular sieve catalyst and a preparation method and application thereof, and comprises the following steps: mixing ZSM-5 molecular sieve, tetraethyl orthosilicate, trichloroacetic acid and water to react, and filtering to obtain an intermediate; mixing the intermediate, a melamine solution and a metal salt solution, and drying and calcining to obtain the metal-modified ZSM-5 molecular sieve catalyst. The prepared catalyst has good catalytic activity, selectivity, stability and the like, and the preparation process is simple and easy to operate.

The invention belongs to the technical field of biology, and relates to extracellular polysaccharide of fermented lactobacillus mucus B44 and application of the extracellular polysaccharide. The invention provides an exopolysaccharide capable of adsorbing heavy metal lead, which is derived from fermentation mucus lactobacillus B44, and the preservation number of the fermentation mucus lactobacillus B44 is CGMCC (China General Microbiological Culture Collection Center) No.17321. The invention further provides a preparation method of the extracellular polysaccharide for adsorbing the heavy metal lead, and the preparation method comprises the following steps: inoculating the fermented lactobacillus mucus B44 into skimmed milk for fermentation to obtain fermented milk; obtaining a fermented supernatant from the fermented milk; carrying out ethanol precipitation on the fermentation supernatant, and collecting the precipitate; and dissolving the precipitate, mixing with trichloroacetic acid, collecting the supernatant, and carrying out dialysis, freeze-drying and other operation treatments to obtain the exopolysaccharide. The extracellular polysaccharide provided by the invention has relatively strong capability of adsorbing heavy metal lead, can relieve intestinal cytotoxicity and oxidative stress caused by lead induction, provides a new solution for treating lead-containing wastewater in an environment and preventing or treating heavy metal lead poisoning, and has a wide application prospect.

The application provides a silver etching solution, a preparation method and application thereof, and the silver etching solution comprises the following components in a weight ratio: 50-80 parts of carboxylic acid; 2-8 parts of sulfonic acid; 5-20 parts of inorganic acid; 0.1-2 parts of chelating agent; 1-5 parts of pH regulator; and 30-50 parts of ultrapure water. The carboxylic acid is one or more of acetic acid, citric acid, tartaric acid, oxalic acid, glycolic acid, benzenehexacarboxylic acid, thiazyl tetraoxide, trichloroacetic acid and lysine. The application further discloses a preparation method of the silver etching solution and application thereof in the field of etching ITO-Ag-ITO composite layers. The silver etching solution has a stable etching rate for ITO-Ag-ITO, does not damage the insulating layer and photoresist, and can be used in the OLED pixel electrode manufacturing process.

The application discloses a method for electrocatalytic hydrogenation dehalogenation of haloacetic acid in water by using a ruthenium / nickel hydroxide catalyst, wherein a conductive material with a surface modified ruthenium / nickel hydroxide is used as a catalytic cathode, the cathode potential is-0.3~‑1.0 V vs. silver / silver chloride reference electrode, and water is used as a hydrogen source, so that the haloacetic acid in water is electrochemically hydrogenated and dehalogenated into acetic acid. Under the condition of the same Ru loading, the catalytic activity of Ru / Ni(OH)2 is higher, the conversion rate of trichloroacetic acid is 9% higher, the acetic acid yield is 14% higher, and the acetic acid selectivity is 5% higher.

The application belongs to the technical field of organic synthesis, and particularly relates to a preparation method of a lamivudine intermediate. The preparation method of the lamivudine intermediate provided by the application comprises the following steps: mixing compound 1, compound 2, triethylamine and an organic solvent to perform a condensation reaction, so as to obtain a mixed solution containing CME beta isomer and CME alpha isomer; dissolving the mixed solution containing CME beta isomer and CME alpha isomer in methanol to perform a salt formation reaction after concentration, and then performing cooling crystallization and filtration, so as to obtain a trichloroacetic acid salt of CME beta isomer; and using an alkaline solution to free the trichloroacetic acid salt of CME beta isomer in methanol, so as to obtain the lamivudine intermediate CME beta isomer. The trichloroacetic acid salt of CME beta isomer is separated by using different solubilities of the trichloroacetic acid salts of alpha and beta isomers, and the CME beta isomer with high purity and high yield is obtained by using the alkaline solution to free.