Method for extraction and purification of lipopolysaccharide from corn dipped in water

A technology for corn soaking water and lipopolysaccharide, which is applied in the field of extracting and purifying lipopolysaccharide from corn soaking water, can solve the problems of unsatisfactory product purity and low lipopolysaccharide content, and achieves the effect of improving the purity and simplifying the operation steps.

Inactive Publication Date: 2004-11-17
HEBEI AGRICULTURAL UNIV.
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  • Summary
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Problems solved by technology

[0002] In the prior art, the ethanol distribution precipitation method is used to extract lipopolysaccharide, which will cause a large amount of protein to precipitate together with lipopolysaccharide, and the content of lipopolysaccharide in the obtained product is very low (<3%). Purification by exchange chromatography, product purity is still unsatisfactory (about 90%)

Method used

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Examples

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Embodiment 1

[0024] Embodiment 1: The method for extracting and purifying lipopolysaccharide in corn soaking water in the pharmaceutical process is as follows:

[0025] 1. Separation of protein and common polysaccharides by isoelectric point method:

[0026] Use saturated sodium hydroxide solution to adjust the pH value of the soaking water to 7.3, separate the soaking solution through a separator, and centrifuge at 3000 rpm for 25 minutes to separate the precipitated supernatant.

[0027] 2. Separation of protein and common polysaccharides by ultrafiltration:

[0028] An ultrafiltration membrane with a molecular weight cut-off of 100,000 was selected to perform ultrafiltration on the above supernatant, and the filtrate was obtained.

[0029] 3. Separation of protein and common polysaccharides by trichloroacetic acid method:

[0030] Add 0.5% trichloroacetic acid by volume to the filtrate, stir for 30 minutes, centrifuge at 3000 rpm for 25 minutes, and separate the supernatant.

[0031]...

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Abstract

The invention discloses a method for extraction and purification of lipopolysaccharide from corn dipped in water which comprises the steps of, first isolating the proteins in the immersion water through the combination of isoelectric point method, hyper-filtration method and trichloroacetic acid method, carrying out filter membrane concentration, further isolating proteins through ethanol precipitation method, then extracting the lipopolysaccharides in the immersion water with activated charcoal, finally purifying the lipopolysaccharides through activated charcoal chromatography and ion-exchange chromatography.

Description

technical field [0001] The invention relates to a method for extracting and purifying lipopolysaccharide, especially a method for extracting and purifying lipopolysaccharide from corn soaking water Background technique [0002] In the prior art, the ethanol distribution precipitation method is used to extract lipopolysaccharide, which will cause a large amount of protein to precipitate together with lipopolysaccharide, and the content of lipopolysaccharide in the obtained product is very low (<3%). Purification by exchange chromatography, the product purity is still unsatisfactory (about 90%). Contents of the invention [0003] The purpose of the present invention is to overcome the above disadvantages and provide a method for extracting and purifying lipopolysaccharide. This method can greatly reduce the extraction and purification steps of lipopolysaccharide, and at the same time significantly improve the purity of the final p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 钱东平曲保雪杨世凤霍晓静牛晓颖孟祥书李瑞奇杨晓娟齐晓娜
Owner HEBEI AGRICULTURAL UNIV.
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