The invention discloses a variable-temperature PCR (
polymerase chain reaction) temperature adjusting device based on an MEMS (micro-electromechanical
system)
thermopile technology, which comprises a radiator, a first base is fixedly mounted on a radiating contact surface of the radiator, a cold panel is fixedly mounted on the upper surface of the first base, and a first film is fixedly mounted on the lower surface of the cold panel and positioned on the inner side of the first base. The variable-temperature PCR temperature adjusting device based on the MEMS
thermopile technology has the advantages of being low in
heating power, short in
response time, small in heat loss, compatible with the
CMOS technology, easy to integrate with other microelectronic devices and the like, an integrated
fluorescent PCR detection
chip of a micro electro
mechanical system (MEMS) is used for detecting the temperature of the temperature-variable PCR temperature adjusting device, and the temperature-variable PCR temperature adjusting device based on the MEMS
thermopile technology has the advantages of being low in
heating power, short in
response time, small in heat loss, compatible with the
CMOS technology, easy to integrate with other microelectronic devices and the like. A microfluidic reaction cavity, a
temperature control module and an optical detection
system are highly integrated on a
single chip platform, so that the integrated operation of
nucleic acid amplification and
fluorescence signal detection is realized. Microminiaturization and integration of PCR
fluorescence detection are achieved through the MEMS technology, the detection efficiency is remarkably improved, the cost is reduced, and the method is suitable for the field of POCT and precision
medical treatment.