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247 results about "Microfluidic channel" patented technology

Multifunctional composite neural electrode system

The embodiment of the invention discloses a multifunctional composite neural electrode system which comprises a supporting structure, a micro-fluidic structure and a flexible electrode structure, the micro-fluidic structure comprises a micro-fluidic channel; a groove is formed in the supporting structure, and at least part of the microfluidic channel is arranged in the groove; the flexible electrode structure is located on the surface of the supporting structure and covers or wraps part of the micro-fluidic channel. By adopting the technical scheme, drug delivery is performed to a target position of a nervous system through the microfluidic structure, electrical recording and chemical regulation and control can be performed on neurons at the same time, and the regulation and control effect of drugs on the neurons can be fed back in real time through electric signals.
Owner:CHINESE INST FOR BRAIN RES BEIJING

Microfluidic devices and methods for forming cell aggregates, and methods for selectively processing cells within cell aggregates.

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.
Owner:CENT NAT DE LA RECH SCI (C N R S) +4

Separated organ-like chip model and use method thereof

The invention relates to the technical field of biomedical engineering, in particular to a separated type organ-like chip model and a use method thereof.The model comprises a chip, an organ-like unit module and a fluid control system, and the use method comprises the steps that firstly, the chip is assembled and prepared; step 2, cell inoculation and culture; step 3, establishing and operating a fluid circulation system; step 4, organ-like unit function detection and data acquisition; 5, analyzing an experimental result and adjusting the model; the model is connected with a micro-fluidic channel through an independent cavity, and the characteristics, cell types and culture conditions of a cell culture bracket can be regulated and controlled according to the characteristics of simulated organs. Meanwhile, the micro-fluidic channel ensures stable and ordered material exchange among the organoid units, and is beneficial to more accurately screening out effective treatment schemes and drug targets.
Owner:SHANDONG FUYOU LIFE SCI CO LTD +1

Sorting control chip and device for hundred-nanoscale particles and sorting control method

The invention relates to a hundred-nanoscale particle sorting control chip and device and a sorting control method, the chip comprises a micro lens, a micro-fluidic channel, a sorting outlet and a sorting inlet, the sorting outlet is a two-fork opening, and the sorting inlet is a three-fork opening and comprises a sample inflow opening and two focusing inflow openings; the sorting inlets and the sorting outlets are formed in the two ends of the micro-fluidic channel respectively, and the micro-lens is arranged at the intersection of the two sorting outlets. The device comprises a chip, a light source, an injection device and an observation platform, light emitted by the light source penetrates through a micro lens and irradiates into a micro-fluidic channel, light force is applied to particles in fluid to be sorted, the particles of different sizes enter different sorting outlets, and finally sorting is achieved. Compared with the prior art, the particle sorting device can realize quick sorting of particles with the diameter of hundreds of nanometers, has the advantages of high sorting efficiency, high control precision and the like, and has important application value in sorting and control of micro-nano-scale particles such as viruses, bacteria and the like.
Owner:TONGJI UNIV

Plugging device pipe air tightness testing method and plugging device pipe air tightness testing system

The invention discloses a plugging device pipe air tightness testing method and system, and relates to the technical field of air tightness test.The plugging device pipe air tightness testing method comprises the steps that a micro-fluidic channel is designed according to a structural assembly of a plugging pipeline of a plugging device, a sensor array is introduced according to detection requirements, and a micro-fluidic chip is constructed; butting the micro-fluidic chip and the plugging pipeline, and generating a parallel test task according to a pipeline assembly mode-inflation condition of the plugging pipeline; performing an inflation test on a plugging pipeline of the plugging device according to the parallel test task, when an airflow balance stage is reached, activating the sensor array to perform directional detection, determining sensing data in the microfluidic channel, and executing data fitting to determine a ventilation circulation state; and according to the ventilation circulation state, evaluating and determining air tightness data. The technical problem that in the prior art, an airtight leakage assembly cannot be accurately identified under different pipeline assembly modes and inflation conditions is solved, and the technical effects of accurately evaluating airtight data and positioning the leakage assembly under various assembly and inflation states are achieved.
Owner:SHANDONG WEIGAO GROUP MEDICAL POLYMER

Microfluidic sampling device capable of quickly and accurately positioning liver cancer tumor biomarkers

The invention discloses a microfluidic sampling device capable of quickly and accurately positioning a liver cancer tumor biomarker, and relates to the technical field of medical instruments, the microfluidic sampling device comprises a chip mounting part, the chip mounting part is formed by assembling a bottom assembly disc, a microfluidic chip, a top plate and an assembly rod, and the microfluidic chip is arranged at the upper part of the bottom assembly disc; through accurate cooperation of the air pressure push rod and an external control system and flow adjustment of a valve control hole in the micro-fluidic chip, it is ensured that liquid inlet conditions for each detection are highly consistent, the detection stability is greatly improved, waste liquid generated in the detection process flows into a waste bin through a sample outlet micro-fluidic channel and a liquid outlet micro-fluidic channel, and the detection efficiency is improved. Due to the fact that the waste bin is independently arranged and is effectively isolated from untreated sample and reagent space, residual pollutants in waste liquid cannot diffuse or leak to other areas, cross contamination between samples is avoided, and specificity and sensitivity of liver cancer tumor biomarker detection are improved.
Owner:THE FIRST AFFILIATED HOSPITAL OF HEBEI NORTH UNIV

Microfluidic modules and interface components

An apparatus may include a microfluidic chip including at least one microfluidic channel and a first at least one electrical interconnect. An apparatus may include a circuit board having at least one interface interconnect and a second at least one electrical interconnect electrically coupled with the first at least one electrical interconnect of the microfluidic chip. An apparatus may include a cartridge enclosing the microfluidic chip and the circuit board, the cartridge including at least one opening allowing access to the at least one microfluidic channel on the microfluidic chip and the at least one interface interconnect.
Owner:VIRGINIA TECH INTELLECTUAL PROPERTIES INC

Hemorheology parameter on-line monitoring and thrombus risk assessment method

The invention relates to a blood rheological parameter online monitoring and thrombus risk assessment method, which comprises the following steps: introducing a blood sample into a microfluidic channel with a periodic geometric mutation structure, applying unsteady shear disturbance through a piezoelectric oscillation device, inducing blood to enter a non-equilibrium rheological state, and exciting a microcosmic coagulation tendency; the red blood cell aggregation behavior, the cell layer slippage phenomenon and the plasma shearing stability are recognized through the photo-thermal interference technology, and the rheological structure layer differentiation characteristics in blood are obtained; the blood subjected to shear disturbance is guided to a micro-channel high-pressure area provided with a flow-limiting threshold structure, and pressure mutation and microcoacervation nucleation generation behaviors induced by local stress disturbance are detected through a nanoscale piezoresistive sensor so as to identify sub-phase change response characteristics of the blood; and constructing a rheological instability index system on the basis of physical response signals obtained by shear disturbance, slippage and variable response, and when linkage abnormity of any two indexes is detected, judging that a high-risk event of thrombosis occurs and outputting a risk prompt signal.
Owner:AFFILIATED HOSPITAL OF JIANGSU UNIV

Encapsulated cell droplet sorting method and system, electronic equipment and storage medium

The embodiment of the invention provides an encapsulated cell droplet sorting method and system, electronic equipment and a storage medium, and belongs to the technical field of intelligent biomedical engineering. The method comprises the following steps: acquiring a sorting area image of a micro-fluidic channel in a micro-fluidic chip; cutting the sorting area image according to a preset cutting frame to obtain a microfluidic droplet image; the microfluidic liquid drop image is input into a cell recognition model based on a convolutional neural network structure to obtain a cell recognition result, and the cell recognition result is used for judging whether cells exist in liquid drops or not; and determining a chip control signal of the micro-fluidic chip according to the cell recognition result so as to collect encapsulated cell droplets in the sorting area. According to the invention, the droplet cell encapsulation efficiency can be improved.
Owner:SHENZHEN RAIN BIOTECHNOLOGY SOLUTIONS CO LTD

In-vitro brain lymphatic system chip construction method and brain lymphatic system chip

ActiveCN120608013ABioreactor/fermenter combinationsBiological substance pretreatmentsContinuous perfusionPulsatile blood flow
The invention belongs to the technical field of organ chips, provides an in-vitro brain lymphatic system chip construction method and a brain lymphatic system chip, and makes up the blank of brain lymphatic system in-vitro model construction. The method comprises the following steps: firstly, carrying out flushing treatment, disinfection and sterilization treatment, rinsing treatment and cell culture on a micro-fluidic channel in the chip; then, in the treated micro-fluidic channel, blood vessel channel cell inoculation and brain parenchyma channel cell inoculation are carried out; and finally, in the blood vessel channel, perfusion is carried out once at a preset flow speed every preset duration, shear force stimulation simulating pulse type blood flow is provided, basic formation of in-vitro cerebral vessels is achieved after continuous perfusion, perfusion is carried out once at a preset flow speed every preset duration, shear force stimulation simulating pulse type blood flow is provided, and in-vitro cerebral vessels are formed. Intermittent liquid flow simulating arterial fluctuation is achieved.
Owner:SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI

Microfluidic sorting chip and method based on synergistic effect of multistage surface acoustic waves and sheath flow

The invention discloses a microfluidic sorting chip and method based on the synergistic effect of multistage surface acoustic waves and sheath flow. The chip comprises a piezoelectric substrate; the PDMS micro-fluidic channel comprises a sample inlet, a sheath flow inlet, a sorting main flow channel for sorting microorganisms, and a collecting unit arranged at the tail end of the sorting main flow channel; the sheath flow inlet is converged from two sides of the sorting main flow channel through two flow paths, and forms hydrodynamic focusing with sample liquid conveyed by the sample inlet in the sorting main flow channel; the number of the multi-stage IDT arrays is four, every two of the multi-stage IDT arrays are symmetrically distributed along the two sides of the sorting main flow channel, the working frequency of the multi-stage IDT arrays is 100-150MHz, and the multi-stage IDT arrays are used for generating a superposition sound field with high energy density. According to the invention, four groups of high-frequency IDT arrays are used for generating a superposed sound field, and a PDMS sorting channel and a double-channel sheath flow system are combined, so that the sorting problem of microorganisms with acoustic contrast factors smaller than 0 is solved.
Owner:SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI

Chemical plating method for plating copper on surface of silicon wafer

The invention discloses a chemical plating method for plating copper on the surface of a silicon wafer, which belongs to the technical field of semiconductor coating, and comprises the following steps: modifying a semiconductor material BaTiO3 nanocrystal by a silane coupling agent to prepare quantum dot ink, spin-coating the quantum dot ink on the surface of a P-type silicon wafer cleaned by RCA to form a quantum dot array, and coating the quantum dot array on the surface of the P-type silicon wafer. The preparation method comprises the following steps: activating in a solution containing copper sulfate and trisodium citrate under the assistance of ultrasonic waves, carrying out chemical copper plating in a micro-fluidic reaction cavity by using an alkaline plating solution containing copper sulfate, disodium ethylene diamine tetraacetate and glyoxylic acid under the assistance of ultrasonic waves and lasers, and finally washing with deionized water and ethanol and drying with nitrogen to obtain a silicon wafer with a copper-plated surface. Through the BaTiO3 quantum dots, the micro-fluidic channel, the pulse liquid supply system and the auxiliary synergistic effect of the ultrasonic wave and the laser array, the copper film which is uniform in thickness, high in adhesive force, good in conductivity and excellent in corrosion resistance can be prepared, and the chemical copper plating efficiency and quality are improved.
Owner:LULIANG UNIV

Microfluidic and SPR battery water state identification method, equipment and medium

The invention discloses a microfluidic and SPR battery water state identification method, equipment and a medium, and relates to the technical field of fuel cells. The method comprises the steps that the hollow optical fiber is embedded into a bipolar plate runner of the fuel cell, a cavity of the hollow optical fiber is a micro-fluidic channel, and the micro-fluidic channel is used for inflow and outflow of liquid in the fuel cell; transmitting the optical signal to the hollow optical fiber, and coating the inner wall of the cavity with a metal layer which is used for exciting SPR (Surface Plasmon Resonance); collecting a spectral signal after interaction of the liquid and the optical signal in real time through a microfluidic channel, and generating a two-dimensional spectrogram; carrying out dimensionality reduction on the two-dimensional spectrogram, extracting gray level image features, and generating a one-dimensional feature vector; and inputting the one-dimensional feature vector into a pre-trained classifier, classifying the state of the fuel cell through supervised and unsupervised mixed learning, and outputting a diagnosis result. According to the method, the state of the liquid in the battery can be accurately diagnosed in real time.
Owner:SHANDONG UNIV OF SCI & TECH

Pipette interface systems and methods for viscous fluid injection

The present disclosure generally relates to microfluidics, and to systems and methods for controlling the introduction of fluids. For example, certain aspects are generally directed to microfluidic devices having ports able to direct the end of a pipette tip into an end portion that is sized so as to allow fluid to flow from the pipette tip into an exit fluidly connected to a microfluidic channel. For example, the port may have a tapered portion that directs the pipette tip to the end portion. The end portion may be sized such that it is difficult for fluid to backflush around the pipette tip, and thus, the fluid is able to flow into microfluidic channels within the device, e.g., without resulting in excessive fluid remaining within the end portion. Other aspects are generally directed to methods of making or using such microfluidic devices, kits including such microfluidic devices, and the like.
Owner:XELLAR LTD

Microfluidic component adapted for characterizing a biological object

The invention relates to a microfluidic component used for electrical impedance measurement through a biological object (O), said microfluidic component comprising: A support in which is made a main microfluidic channel (C_1) defining a path for the circulation of a fluid containing the biological object (O), A trapping device positioned in the main microfluidic channel (C_1) on the circulation path of said biological object, At least one first electrode and a second electrode intended to be each set at a distinct electrical potential, in order to perform said impedance measurement, Said trapping device being composed of at least one first central pillar (P_1) and a second central pillar (P_2) positioned inside the microfluidic channel, The first central pillar being configured to form the first electrode and the second central pillar being configured to form the second electrode.Figure to be published with the abbreviation: Figure 2.
Owner:COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES

High-sensitivity differential microfluid sensor based on microstrip line

The invention relates to a high-sensitivity differential microfluid sensor based on a microstrip line, which is used for measuring the concentration of a water-ethanol mixed solution, and comprises the microstrip line configured with a symmetrical shunt / combiner, a pair of split ring resonators with bent grooves, and a microfluid channel, a dielectric layer and a metal ground which are arranged at the tops of the split ring resonators from the top layer to the bottom layer. Wherein the bent groove parts of the two SRRs are areas with the maximum electric field intensity, and a micro-fluidic channel made of polydimethylsiloxane (PDMS) is placed in the areas. A sensor parameter curve is measured by a vector network analyzer (VNA). The suggested size of the sensor is 30 * 20 mm < 2 >, and the working frequency is 4.8 GHz. Experimental results show that the resonant frequency of the water-ethanol mixture with the concentration range of 0-100% has linear shift of 1250 MHz, and the average sensitivity Savf of detection is 0.987%. The sensor has the characteristics of compactness, high sensitivity, high measurement precision, no environmental interference and the like, and has wide application prospects in the fields of biomedical detection, material analysis, environmental monitoring and the like.
Owner:CHINA THREE GORGES UNIV

Microfluidic system for controlling the movement of charged particles and a control method using the same

PendingUS20260183764A1CapacitanceChemical physics
A microfluidic system for controlling movement of charged particles, including a microfluidic channel, at least three electrodes, a plurality of conductor leads and at least two drive power supplies. Each electrode is in electrical contact with the electrolyte, and a pseudo-capacitance, a double-layer capacitance or a combination thereof is formed at an electrode-electrolyte interface. Each drive power supply periodically produces a voltage or current excitation, and is connected to one or more electrodes via the conductor leads. Each electrode continuously undergoes charging-discharging alternating cycles to form a traveling-wave electric field in the microfluidic channel. A maximum charge capacity of each electrode is greater than a total amount of charges transferred thereon during a charging or discharging process. A control method based on the microfluidic system is also provided.
Owner:ZHUHAI JIEYI BIOTECHNOLOGY SCI & TECH CO LTD

Hemolysis analyzer based on integrated optical waveguide sensor and preparation method thereof

The application discloses a hemolysis analyzer based on an integrated optical waveguide sensor and a preparation method thereof, and comprises an integrated photon waveguide, a nano filter and a micro-fluidic channel, wherein the integrated photon waveguide is a single waveguide with a middle spiral type and coupling gratings on both sides. The hemolysis analyzer fully utilizes the advantages of high sensitivity and small volume of the integrated optical waveguide sensor, and realizes sample-free preparation by combining the methods of instantaneous absorption and hemoglobin filtration, and can quickly and accurately detect hemolysis and the degree of hemolysis.
Owner:JIAXING RES INST ZHEJIANG UNIV

Intelligent collagen monitoring method and system of integrated microsensor

PendingCN121987148AAchieve multiple recycling usesRecovery class solid stateAnalysing solids using sonic/ultrasonic/infrasonic wavesOrgan movement/changes detectionCollagen breakdownCollagenan
The invention relates to the technical field of collagen intelligent monitoring, and particularly discloses an intelligent collagen monitoring method and system integrated with a microsensor. Enzyme response hydrogel with switchable states is anchored to the skin through a microneedle array, a high-frequency acoustic impedance analysis module is used for continuously probing the acoustic impedance change of the hydrogel, and the collagen monitoring accuracy is improved. Identifying a collagen degradation event; once collagen degradation is detected, the system activates the micro-regeneration unit, a cleaning solution and a regeneration solution are conveyed through a microfluidic channel, a broken peptide chain is removed, a hydrogel cross-linked network is reconstructed, and functional regeneration of the sensing unit is realized; the system further comprises a regeneration state confirmation and cycle reset mechanism, and the reliability of each monitoring cycle and the consistency of data references are ensured. According to the method, the limitation of a traditional monitoring method is overcome, multiple times of recycling of a single sensor is achieved, the credibility and consistency of long-term monitoring data are improved, and the method has important significance on skin health management and senescence research.
Owner:PINEN BIOTECHNOLOGY (SHENZHEN) CO LTD

Endotoxin detection system

The utility model relates to the technical field of endotoxin monitoring, in particular to an endotoxin detection system. The utility model aims to solve the problem that the detection efficiency of the creotoxin is low. The endotoxin detection system comprises a light source emitting device, an incident light lead, a microfluidic channel, an optical fiber biochemical sensor, an emergent light lead and a spectral analysis device, the optical fiber biochemical sensor is arranged inside the microfluidic channel, the optical fiber biochemical sensor is provided with a sample groove, the microfluidic channel is provided with at least two sample inlets and at least one sample outlet, and a microfluidic channel area between any sample inlet and any sample outlet covers the sample groove; one end of the incident light lead is connected with the light source generating device, the other end of the incident light lead penetrates into the micro-fluidic channel and is connected to the optical fiber biochemical sensor, one end of the emergent light lead is connected with the optical fiber biochemical sensor, and the other end of the emergent light lead penetrates out of the micro-fluidic channel and is connected to the spectral analysis device.
Owner:JINAN UNIVERSITY

Renal function dry-type biochemical detection chip and use method thereof

The invention belongs to the technical field of kidney disease detection, and particularly relates to a renal function dry-type biochemical detection chip and a use method thereof. The chip is combined with a dry biochemical reaction layer through a micro-fluidic technology, and can be used for synchronously detecting renal function markers such as urine creatinine, urea nitrogen and cystatin C. The chip adopts a multi-layer modular lamination design, and sequentially comprises a bottom plate, an electrode layer, a detection layer, a binding layer and a sample adding layer from bottom to top, and each layer is made of a high-molecular polymer with excellent biocompatibility, so that the detection process is not interfered, and the result is accurate and reliable. Sample flow is accurately controlled through the microfluidic channel, multi-index parallel detection is realized, and the detection efficiency is improved. A micro sensor is arranged in the chip, the reaction process is monitored in real time, and data accuracy is ensured. The system has the characteristics of portability and quick response, is suitable for clinical and family self-test, and provides a convenient and efficient solution for renal function monitoring.
Owner:SHANGHAI KEELING INTELLIGENT TECH CO LTD

Octopus bionic sucker flexible negative voltage electrode patch for nerve electrical regulation and control

The invention discloses an octopus bionic sucker flexible negative voltage electrode patch for nerve electrical regulation and control. The octopus bionic sucker flexible negative voltage electrode patch comprises a flexible substrate, an octopus bionic sucker array and a microneedle electrode array, a patterned micro-fluidic channel is arranged in the flexible substrate, and a first micro-fluidic hole array is arranged on the surface of the flexible substrate; the octopus bionic suction cup array has flexibility and adhesion and comprises a suction cup array, an electrode hole array arranged on the suction cup array and a second micro-fluidic hole array, and the second micro-fluidic hole array and the first micro-fluidic hole array correspondingly form a micropore channel; the microneedle electrode array is inserted into the electrode hole array; when the electrode patch is used for nerve electric regulation and control, under the action of pre-tightening force, the suction cup adheres to the skin and is preliminarily attached to the skin, the micro-needle electrode array on the suction cup acts on the cortex, gas in the suction cup is extracted through the micro-fluidic channel and the micropore channel to obtain an external negative pressure source, stable adsorption force is provided through negative pressure, sweat on the surface of the human body is discharged, and therefore the skin can be absorbed. And long-term high-quality nerve electric regulation and control are realized.
Owner:ZHEJIANG UNIV

Process for the synthesis of benzimidazole michael addition derivatives in a continuous flow reactor

The application provides a method for synthesizing benzimidazole Michael addition derivatives in a continuous flow reactor, and 2-methylbenzimidazole and alpha, beta-unsaturated olefins are used to catalyze the online synthesis of benzimidazole Michael addition derivatives in a microfluidic channel reactor by using a lipase Lipozyme RM IM. The method not only greatly shortens the reaction time, but also has high conversion rate; meanwhile, the Michael addition reaction of 2-methylbenzimidazole and alpha, beta-unsaturated olefins is catalyzed by using the economical lipase Lipozyme RM IM in methanol, the reaction cost is reduced, the post-processing step is simplified, and the method has the advantages of green environmental protection, economy and efficiency.
Owner:ZHEJIANG UNIV OF TECH

A hundred nanometer level particle sorting manipulation chip, device and sorting manipulation method

The present application relates to a kind of hundred nanometer level particle sorting manipulation chip, device and sorting manipulation method, the chip includes microlens, microfluidic channel, sorting outlet and sorting inlet, the sorting outlet is bifurcation, the sorting inlet is trifurcation, including sample flow inlet and 2 focus flow inlets;Sorting inlet and sorting outlet are respectively arranged at the two ends of microfluidic channel, and microlens is arranged at the intersection of 2 sorting outlets.The device includes chip, light source, injection device and observation platform, and the light emitted by light source is irradiated into microfluidic channel through microlens, and light force is applied to the particle in the fluid to be sorted, and different size particles enter different sorting outlets, finally realize sorting.Compared with prior art, the present application can realize the rapid sorting of hundred nanometer level particle, with the advantages of high sorting efficiency, high control precision, etc., and has important application value for the sorting and manipulation of micro-nano scale particles such as virus and bacteria.
Owner:TONGJI UNIV

Microfluidic channel for extruding cells, microfluidic chip and application thereof

This invention relates to the field of biomedical technology, specifically to a microfluidic channel, a microfluidic chip, and their applications for squeezing cells. The microfluidic channel is composed of several continuously arranged cell squeezing units. Each cell squeezing unit consists of a main channel and a squeezing region. The main channel is composed of connecting sections and curved sections. The squeezing region includes a first squeezing section, a squeezing slit, and a second squeezing section. The width of the first squeezing section gradually decreases from the main channel, reaching its minimum at the squeezing slit, and then gradually increases towards the second squeezing section until it matches the width of the main channel. The squeezing slit is rectangular, with a width of 4-10 μm and a length of 20-60 μm. The microfluidic channel provided by this invention applies relatively uniform and dispersed mechanical stress to the cell membrane, reducing cell breakage and mortality rates, and allowing cells to restore their integrity through the cell membrane's self-repair mechanism after squeezing.
Owner:SHANGHAI UNIV

Three-dimensional printed articles and methods of manufacture

Three-dimensionally (3D) printed, wearable, electronic sensing articles and methods of manufacture are disclosed. In one example embodiment, the present disclosure provides A wearable article that includes a three-dimensionally printed base, wherein the base of the article comprises a three-dimensionally printed mesh which is formed of an elastomeric polymer composition. The wearable article also includes a microfluidic channel coupled to the mesh, the microfluidic channel having an inlet and an outlet disposed at opposite ends of the channel, wherein the microfluidic channel is curved in a form of a serpentine shape; and wherein the serpentine shape is provided by a plurality of 180-degree semi-circular segments.
Owner:THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIV OF ARIZONA

Electrochemical sensing elisa reagent card

The application provides an electrochemical sensing ELISA reagent card, comprising a microfluidic channel body and an electrochemical sensor body which are bonded together, wherein: a plurality of "annular" liquid flow channels are arranged on the microfluidic channel body, each "annular" liquid flow channel is formed by a working electrode covering flow channel and a counter electrode and a reference electrode covering flow channel in series, and the "annular" liquid flow channels are communicated through a common flow channel; the electrochemical sensor body comprises a flat plate substrate and a plurality of screen-printed electrodes which are printed in parallel on the flat plate substrate, and each screen-printed electrode comprises a working electrode, a counter electrode and a reference electrode. The electrochemical sensing ELISA reagent card combines an electrochemical biosensor and a microfluidic chip, and solves the technical problems of large sample / reagent consumption, single detection index and liquid volume influence on absorbance measurement of a traditional ELISA reagent kit.
Owner:GENERAL HOSPITAL OF PLA

An organ-on-a-chip

The application provides an organ chip, which comprises, from top to bottom, a liquid collecting layer (1), a pressing layer (2), a glue injection layer (3), a tissue layer (4), a film layer (5) and an oxygen control layer (6). The application provides a novel organ chip, which comprises a film layer and an oxygen control layer, is used for making oxygen pass through the film layer and be eliminated by an oxygen absorbent in the oxygen control layer, liquid cannot pass through the film layer, so that the liquid circulates or is supplemented in the microfluidic channel, and the oxygen concentration in the culture solution is made to be closer to the real physiological environment in the human body based on the cavity and the through structure formed by laminating and sealing of the layers. In addition, a plurality of molds are arranged, so that a plurality of cell models can be simultaneously cultured, and a high-throughput culture effect is realized.
Owner:SHANGHAI BIOCHIP

Alignment and fusion of cells with an electrical field

A method of alignment and fusion of cells with an electrical field includes firing a first fluid dispenser of an electrofusion device until a first impedance sensor in a first microfluidic channel of the electrofusion device detects a presence of a first cell. The method includes firing a second fluid dispenser of the electrofusion device until a second impedance sensor in a second microfluidic channel of the electrofusion device detects a presence of a second cell. The method includes moving the first cell and the second cell into a merging chamber of the electrofusion device by firing a third fluid dispenser of the electrofusion device, in response to alignment of the first cell in the first microfluidic channel with the second cell in the second microfluidic channel. The method further includes fusing the first cell and the second cell in the merging chamber by creating an electrical field in the merging chamber.
Owner:HEWLETT PACKARD DEVELOPMENT COMPANY LP

Microfluidic synthesis chip cartridge

PCT designated stageWO2026177586A1Microfluidic channelBiology
The present invention relates to a microfluidic synthesis chip cartridge on which a synthesis chip including a microfluidic chamber formed in a predetermined shape is mounted, so that two or more fluids that do not mix well with each other are uniformly mixed to generate a synthesized sample. The purpose of the present invention is to provides a microfluidic synthesis chip cartridge wherein: the microfluidic synthesis chip cartridge includes a plate-shaped member on which two or more synthesis chips having microfluidic channels of a predetermined shape for mixing or reacting two or more liquid samples are mounted in parallel, thereby providing a structure and arrangement capable of dramatically increasing production per unit time despite the synthesis chips having microfluidic channels; and the raw-material supply timings of the two or more synthesis chips are aligned solely by changing the shapes of the microfluidic channels rather than by a separate electronically precise mechanism, thereby enabling synchronization among a plurality of synthesis chips with an inexpensive and simple structure.
Owner:MEPSGEN CO LTD