342 results about "Microfluidic channel" patented technology

The invention relates to the technical field of fluid control, and discloses a fluid control intelligent adjusting method and system for a micro-fluidic chip, and the method comprises the steps: carrying out the data collection of a self-adaptive fluid controller, a micro-fluidic channel pressure sensor array, a temperature sensor array and a flow sensor array based on the micro-fluidic chip; obtaining real-time fluid parameter monitoring data; performing feature extraction to obtain a multi-dimensional fluid feature matrix; establishing a three-dimensional fluid model of the microfluidic channel, and solving to obtain flow field distribution data; inputting the flow field distribution data into a Kriging agent model, establishing a mapping model of channel width, depth, length and fluid control performance, and calculating to obtain an optimal geometric parameter combination; and performing multi-level calculation on the fluid pressure uniformity, the temperature distribution uniformity and the flow stability to obtain a hierarchical fluid parameter control strategy. According to the invention, the problem of multi-physics field coupling is effectively solved, the calculation complexity of the control system is reduced, and the execution efficiency of the control strategy is improved.

The invention provides an intelligent algae analysis method and system, and the method comprises the steps: S1, collecting a microscopic image in a microfluidic channel through a flow analyzer based on a target water body; s2, accurately marking the category, the position and the boundary characteristics of the microalgae in the microscopic image by using a marking tool supporting image segmentation and classification in combination with corresponding microalgae classification data; s3, based on the pre-training weight of the Ground SAM, performing fine tuning optimization on the model by using a transfer learning method so as to adapt to microalgae detection; s4, preprocessing the microscopic image acquired in the step S1; s5, for the preprocessed microscopic image, on the basis of the training model in the step S3, realizing target detection and segmentation of microalgae in the microscopic image by using Ground SAM; and S6, counting the types and volumes of the microalgae, identifying the types of the algae, and calculating the density of the algae. Under the support of the flow analyzer, each microalgae cell is accurately detected and counted, so that more detailed algae density data is obtained.

The invention provides a neuromuscular multi-element coupled organoid micro-fluidic chip, a device and a method. Relates to the technical field of organoid micro-fluidic chips. A first culture chamber, a second culture chamber and a third culture chamber are arranged in a culture layer of the chip; the first culture room is used for culturing nerve cells; the second culture room is used for culturing muscle cells; a microfluidic channel for communicating the first culture chamber with the second culture chamber is arranged between the first culture chamber and the second culture chamber; the third culture room is arranged on the micro-fluidic channel and is used for culturing a neuromuscular joint formed by convergence of axons of nerve cells and muscle cells; a microelectrode array plate is arranged in the circuit layer and is used for performing electrical stimulation and electrophysiological function detection on neuromuscular cells. Through the chip, co-culture of in-vitro nerves and muscle organs can be realized, a complex interaction relationship in a neuromuscular system can be accurately simulated, and the chip can also be used for constructing various neuromuscular related disease models.

The invention discloses a wearable sweat glucose detection system based on multi-shell metal organic framework encapsulated enzyme and a preparation method of the wearable sweat glucose detection system. The detection system comprises a sweat collection and sensing module, an electrochemical detection module and an iontophoresis module, the sweat collecting and sensing module comprises a micro-fluidic channel and a screen-printed electrode; the micro-fluidic channel is used for collecting sweat; the screen-printed electrode comprises a working electrode for detecting glucose, a counter electrode and a reference electrode to form a three-electrode system; wherein the working electrode for detecting the glucose is provided with a functional layer, and the functional layer is modified by multi-shell metal organic framework layered encapsulation enzyme for detecting the glucose and chitosan for fixing the multi-shell metal organic framework layered encapsulation enzyme; the electrochemical detection module is used for obtaining electrochemical sensing detection data; and the iontophoresis module is used for outputting an adjustable constant current source to enable iontophoresis to stimulate skin to sweat. The portable long-time continuous detection of sweat glucose can be realized, and the detection method is rapid and convenient.

Disclosed are a standing wave acoustofluidic device for sorting exosomes in body fluids and a method for using same. The standing wave acoustofluidic device for sorting exosomes in body fluids comprises an upper microfluidic chip and a lower acoustic wave excitation apparatus. The microfluidic chip comprises a channel chip and a cover plate, and the acoustic wave excitation apparatus comprises a piezoelectric transducer and an acoustic wave coupling liquid layer. The piezoelectric transducer is reversibly connected to the cover plate by means of the acoustic wave coupling liquid layer. The piezoelectric transducer is a piezoelectric ceramic. The channel chip is provided with a body fluid sorting channel, and the cover plate is provided with a micro-channel groove array structure corresponding to the body fluid sorting channel. The micro-channel groove array structure adjusts acoustic waves excited by the piezoelectric transducer, such that the acoustic waves are modulated in a space composed of the channel chip and the cover plate to form a required standing wave acoustic field. The present invention has advantages such as not affecting cell activity, being unaffected by temperature and the like, offering a stable sorting process, a simple manufacturing process, and low regulation difficulty, and eliminating the need to match the width, depth, and the like of microfluidic channels with the half-wavelength of acoustic waves.

The utility model relates to a flexible wearable liquid metal antenna and wearable electronic equipment, and belongs to the technical field of wearable antennas. Comprising a dielectric substrate, a grounding metal sheet, a feed port metal sheet, a metal floor and an inverted F-shaped groove, according to the dual-band wrist type PIFA antenna, a flexible material PDMS is used as a dielectric substrate, liquid metal EGaIn is used as a radiation material, and the EGaIn is wrapped in a microfluidic channel formed by the PDMS, so that the wearable antenna working at two frequency bands of 2.4 GHz and 5.8 GHz is realized. Compared with a traditional solid metal wearable antenna, the wearable antenna designed on the basis of liquid metal can well receive signals, material fatigue or cracking cannot be caused by repeated bending, and the wearable antenna has high self-repairing capacity and has more advantages in the wearable aspect.

The invention belongs to the technical field of medical detection, and discloses a blood disease high-throughput detection method based on a micro-fluidic chip and SERS (Surface Enhanced Raman Scattering) detection, which comprises the following steps: introducing a blood sample into the micro-fluidic chip with a micro-fluidic channel; the method comprises the following steps: performing SERS (Surface Enhanced Raman Scattering) detection on a blood sample through a nano-structure surface preset in a micro-fluidic channel; according to the present invention, with the microfluidic chip and the SERS technology, the high-throughput detection of the blood sample is achieved, the SERS spectrum data is collected and analyzed, the disease marker in the blood is identified, and the high-throughput detection of the blood sample is achieved through the combination of the microfluidic chip and the SERS technology. A plurality of micro-channels on the micro-fluidic chip can process a plurality of samples in parallel, the detection efficiency is remarkably improved, and the strength of Raman scattering signals can be remarkably improved by utilizing the surface enhancement effect of the SERS technology, so that high-sensitivity detection of blood disease markers is realized, and the detection cost is reduced. Accurate control and efficient separation of trace samples are achieved through the micro-fluidic chip, complex pretreatment steps are not needed, and the operation process is simplified.

A method for constructing a microfluidic bone organoid-on-chip can continuously observe the impacts of different external factors on a bone tissue structure based on the “ternary regulation theory” of bone angiogenesis-bone resorption-osteogenesis coupling. The method includes: (1) building a visual three-dimensional cell cultivation platform to continuously observe impacts of different external factors on a ternary regulation theory based on angiogenesis of osteoclast precursors, osteoblasts and osteoclasts, such that a user is allowed to directly observe interactions among various kinds of cells; (2) designing microfluidic channels in a chip to allow a reagent involved to present a concentration gradient distribution in the chip; and (3) adding three bioinks including osteoblasts, osteoclasts and vascular endothelial cells respectively, so as to reflect the three cell components individually, in pairs, and as a whole in the chip. An apparatus for constructing a microfluidic bone organoid-on-chip is further provided.

The embodiment of the invention discloses a multifunctional composite neural electrode system which comprises a supporting structure, a micro-fluidic structure and a flexible electrode structure, the micro-fluidic structure comprises a micro-fluidic channel; a groove is formed in the supporting structure, and at least part of the microfluidic channel is arranged in the groove; the flexible electrode structure is located on the surface of the supporting structure and covers or wraps part of the micro-fluidic channel. By adopting the technical scheme, drug delivery is performed to a target position of a nervous system through the microfluidic structure, electrical recording and chemical regulation and control can be performed on neurons at the same time, and the regulation and control effect of drugs on the neurons can be fed back in real time through electric signals.

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.

The invention belongs to the technical field of biomedical detection, and particularly discloses a surrounding type multi-neurotransmitter recognition biological mixed synapse system which is composed of a flexible biological synapse device and a microfluidic channel, the microfluidic channel simulates a biological synapse gap and controls transmission and diffusion of different neurotransmitters, and the flexible biological synapse device and the microfluidic channel are used for detecting the biological synapse gap. The releasing and receiving processes of neurotransmitters in a biological synaptic gap are simulated; according to the flexible biological synapse device, direct interaction between biological tissues and equipment is realized, and the change of long-term synapse weight is simulated by utilizing a redox reaction of a neurotransmitter at a grid electrode, so that the dynamic adaptability and the information processing capability of a system are improved; when neurons generate peak signals containing different neurotransmitters, the system can accurately distinguish and identify the signals and efficiently transmit the signals; therefore, the biological mixed synaptic system disclosed by the invention becomes an ideal multi-neurotransmitter monitoring tool and can be widely applied to neuroscience research and clinical diagnosis.

The invention relates to the technical field of biomedical engineering, in particular to a separated type organ-like chip model and a use method thereof.The model comprises a chip, an organ-like unit module and a fluid control system, and the use method comprises the steps that firstly, the chip is assembled and prepared; step 2, cell inoculation and culture; step 3, establishing and operating a fluid circulation system; step 4, organ-like unit function detection and data acquisition; 5, analyzing an experimental result and adjusting the model; the model is connected with a micro-fluidic channel through an independent cavity, and the characteristics, cell types and culture conditions of a cell culture bracket can be regulated and controlled according to the characteristics of simulated organs. Meanwhile, the micro-fluidic channel ensures stable and ordered material exchange among the organoid units, and is beneficial to more accurately screening out effective treatment schemes and drug targets.

The invention relates to a hundred-nanoscale particle sorting control chip and device and a sorting control method, the chip comprises a micro lens, a micro-fluidic channel, a sorting outlet and a sorting inlet, the sorting outlet is a two-fork opening, and the sorting inlet is a three-fork opening and comprises a sample inflow opening and two focusing inflow openings; the sorting inlets and the sorting outlets are formed in the two ends of the micro-fluidic channel respectively, and the micro-lens is arranged at the intersection of the two sorting outlets. The device comprises a chip, a light source, an injection device and an observation platform, light emitted by the light source penetrates through a micro lens and irradiates into a micro-fluidic channel, light force is applied to particles in fluid to be sorted, the particles of different sizes enter different sorting outlets, and finally sorting is achieved. Compared with the prior art, the particle sorting device can realize quick sorting of particles with the diameter of hundreds of nanometers, has the advantages of high sorting efficiency, high control precision and the like, and has important application value in sorting and control of micro-nano-scale particles such as viruses, bacteria and the like.

The invention discloses a plugging device pipe air tightness testing method and system, and relates to the technical field of air tightness test.The plugging device pipe air tightness testing method comprises the steps that a micro-fluidic channel is designed according to a structural assembly of a plugging pipeline of a plugging device, a sensor array is introduced according to detection requirements, and a micro-fluidic chip is constructed; butting the micro-fluidic chip and the plugging pipeline, and generating a parallel test task according to a pipeline assembly mode-inflation condition of the plugging pipeline; performing an inflation test on a plugging pipeline of the plugging device according to the parallel test task, when an airflow balance stage is reached, activating the sensor array to perform directional detection, determining sensing data in the microfluidic channel, and executing data fitting to determine a ventilation circulation state; and according to the ventilation circulation state, evaluating and determining air tightness data. The technical problem that in the prior art, an airtight leakage assembly cannot be accurately identified under different pipeline assembly modes and inflation conditions is solved, and the technical effects of accurately evaluating airtight data and positioning the leakage assembly under various assembly and inflation states are achieved.

The invention discloses a micro-fluidic photoelectrochemical immunosensor based on a ZnIn2S4-In2S3 heterojunction photosensitive material and application of the micro-fluidic photoelectrochemical immunosensor based on the ZnIn2S4-In2S3 heterojunction photosensitive material, synthesized In2S3 is used as a precursor, and the ZnIn2S4-In2S3 heterojunction photosensitive material is synthesized in one step. A ZnIn2S4-In2S3 heterojunction photosensitive material is sprayed on the tin oxyfluoride electrode subjected to photoetching of the micro-fluidic channel, and an ascorbic acid solution is used as a solution for enhancing a light absorption signal; the preparation method comprises the following steps: preparing a placenta growth factor monoclonal antibody, activating amino and carboxyl of the sensor through chitosan (CTS) and glutaraldehyde (GA), and fixing the placenta growth factor monoclonal antibody on a heterojunction photosensitive material through a covalent bond to obtain the microfluidic photoelectrochemical immunosensor. And the kit has good clinical sample detection capability and has important clinical application value for human precision medical research.

The invention relates to the technical field of endotoxin detection, in particular to an endotoxin quantitative detection method and a detection system. The invention aims to improve the detection sensitivity and convenience of endotoxin and reduce the use amount of a sample. A first purpose of the present invention is to provide an endotoxin quantitative detection method, which comprises: respectively injecting a standard tachypleus amebocyte lysate solution and endotoxin standard solutions with different concentrations into a microfluidic channel embedded with an optical fiber biochemical sensor according to a specific volume ratio, and inputting signal light into the optical fiber biochemical sensor, acquiring spectral data output when the optical fiber biochemical sensor contains different standard mixed solutions, drawing an endotoxin concentration logarithm-time fitting curve chart, then replacing the endotoxin standard solution with a to-be-detected sample, repeating the steps, extracting corresponding time parameters, and comparing the time parameters with the fitting curve chart, so as to obtain the endotoxin content in the to-be-detected sample solution. The second purpose of the invention is to provide an endotoxin quantitative detection system.

The invention discloses an oscillation microbubble chip, a separation system and an acoustic manipulation method, the device utilizes the acoustic eddy current effect induced by oscillation microbubbles to realize micro-nano particle acoustic manipulation, the chip structure and operation are simple, the biocompatibility is high, and by designing the chip structure, due to the breathable and water-repellent characteristics of PDMS and the surface tension of fluid, the micro-nano particle acoustic manipulation can be realized. Alternatively arranged equilateral triangle column cavities with different sizes are formed on the side wall of the PDMS microfluidic channel to anchor bubbles. Bubble oscillation is driven by bulk acoustic waves generated by exciting a piezoelectric transducer through a signal generator, symmetrical vortexes are formed on the surfaces of the bubbles through secondary acoustic radiation force generated by bubble oscillation, then large-size particles are captured, and small-size particles flow to an outlet along with flowing under the action of drag force. Gradient separation is adopted to separate small particles from a mixed solution and enrich large particles, enrichment, separation and other control of microparticles are achieved through the acoustic vortex field effect of a specific structure, great potential is achieved in biological research, the structure is simple, the equipment cost is low, and integration is easy.

The invention discloses a microfluidic sampling device capable of quickly and accurately positioning a liver cancer tumor biomarker, and relates to the technical field of medical instruments, the microfluidic sampling device comprises a chip mounting part, the chip mounting part is formed by assembling a bottom assembly disc, a microfluidic chip, a top plate and an assembly rod, and the microfluidic chip is arranged at the upper part of the bottom assembly disc; through accurate cooperation of the air pressure push rod and an external control system and flow adjustment of a valve control hole in the micro-fluidic chip, it is ensured that liquid inlet conditions for each detection are highly consistent, the detection stability is greatly improved, waste liquid generated in the detection process flows into a waste bin through a sample outlet micro-fluidic channel and a liquid outlet micro-fluidic channel, and the detection efficiency is improved. Due to the fact that the waste bin is independently arranged and is effectively isolated from untreated sample and reagent space, residual pollutants in waste liquid cannot diffuse or leak to other areas, cross contamination between samples is avoided, and specificity and sensitivity of liver cancer tumor biomarker detection are improved.

An apparatus may include a microfluidic chip including at least one microfluidic channel and a first at least one electrical interconnect. An apparatus may include a circuit board having at least one interface interconnect and a second at least one electrical interconnect electrically coupled with the first at least one electrical interconnect of the microfluidic chip. An apparatus may include a cartridge enclosing the microfluidic chip and the circuit board, the cartridge including at least one opening allowing access to the at least one microfluidic channel on the microfluidic chip and the at least one interface interconnect.

The invention relates to a blood rheological parameter online monitoring and thrombus risk assessment method, which comprises the following steps: introducing a blood sample into a microfluidic channel with a periodic geometric mutation structure, applying unsteady shear disturbance through a piezoelectric oscillation device, inducing blood to enter a non-equilibrium rheological state, and exciting a microcosmic coagulation tendency; the red blood cell aggregation behavior, the cell layer slippage phenomenon and the plasma shearing stability are recognized through the photo-thermal interference technology, and the rheological structure layer differentiation characteristics in blood are obtained; the blood subjected to shear disturbance is guided to a micro-channel high-pressure area provided with a flow-limiting threshold structure, and pressure mutation and microcoacervation nucleation generation behaviors induced by local stress disturbance are detected through a nanoscale piezoresistive sensor so as to identify sub-phase change response characteristics of the blood; and constructing a rheological instability index system on the basis of physical response signals obtained by shear disturbance, slippage and variable response, and when linkage abnormity of any two indexes is detected, judging that a high-risk event of thrombosis occurs and outputting a risk prompt signal.

The utility model provides a micro-fluidic chip for real-time high-flux pretreatment of various mycotoxins, and belongs to the field of micro-fluidic chip manufacturing technology and rapid detection. The micro-fluidic chip is a centrifugal micro-fluidic chip, the micro-fluidic chip comprises a lower-layer substrate provided with a micro-fluidic channel and an upper-layer substrate bonded with the lower-layer substrate, and the lower-layer substrate is provided with a sample mixing area, a centrifugal area, a diluent chamber, a siphon micro-valve and a dilution area which are communicated in sequence; the micro-fluidic chip is rapid and portable, does not need complex instruments, can be used for rapid in-situ pretreatment of various mycotoxins, and can be applied to instant pretreatment of various mycotoxins in grains.

The embodiment of the invention provides an encapsulated cell droplet sorting method and system, electronic equipment and a storage medium, and belongs to the technical field of intelligent biomedical engineering. The method comprises the following steps: acquiring a sorting area image of a micro-fluidic channel in a micro-fluidic chip; cutting the sorting area image according to a preset cutting frame to obtain a microfluidic droplet image; the microfluidic liquid drop image is input into a cell recognition model based on a convolutional neural network structure to obtain a cell recognition result, and the cell recognition result is used for judging whether cells exist in liquid drops or not; and determining a chip control signal of the micro-fluidic chip according to the cell recognition result so as to collect encapsulated cell droplets in the sorting area. According to the invention, the droplet cell encapsulation efficiency can be improved.

The invention belongs to the technical field of organ chips, provides an in-vitro brain lymphatic system chip construction method and a brain lymphatic system chip, and makes up the blank of brain lymphatic system in-vitro model construction. The method comprises the following steps: firstly, carrying out flushing treatment, disinfection and sterilization treatment, rinsing treatment and cell culture on a micro-fluidic channel in the chip; then, in the treated micro-fluidic channel, blood vessel channel cell inoculation and brain parenchyma channel cell inoculation are carried out; and finally, in the blood vessel channel, perfusion is carried out once at a preset flow speed every preset duration, shear force stimulation simulating pulse type blood flow is provided, basic formation of in-vitro cerebral vessels is achieved after continuous perfusion, perfusion is carried out once at a preset flow speed every preset duration, shear force stimulation simulating pulse type blood flow is provided, and in-vitro cerebral vessels are formed. Intermittent liquid flow simulating arterial fluctuation is achieved.

The invention discloses a microfluidic sorting chip and method based on the synergistic effect of multistage surface acoustic waves and sheath flow. The chip comprises a piezoelectric substrate; the PDMS micro-fluidic channel comprises a sample inlet, a sheath flow inlet, a sorting main flow channel for sorting microorganisms, and a collecting unit arranged at the tail end of the sorting main flow channel; the sheath flow inlet is converged from two sides of the sorting main flow channel through two flow paths, and forms hydrodynamic focusing with sample liquid conveyed by the sample inlet in the sorting main flow channel; the number of the multi-stage IDT arrays is four, every two of the multi-stage IDT arrays are symmetrically distributed along the two sides of the sorting main flow channel, the working frequency of the multi-stage IDT arrays is 100-150MHz, and the multi-stage IDT arrays are used for generating a superposition sound field with high energy density. According to the invention, four groups of high-frequency IDT arrays are used for generating a superposed sound field, and a PDMS sorting channel and a double-channel sheath flow system are combined, so that the sorting problem of microorganisms with acoustic contrast factors smaller than 0 is solved.

The invention discloses a chemical plating method for plating copper on the surface of a silicon wafer, which belongs to the technical field of semiconductor coating, and comprises the following steps: modifying a semiconductor material BaTiO3 nanocrystal by a silane coupling agent to prepare quantum dot ink, spin-coating the quantum dot ink on the surface of a P-type silicon wafer cleaned by RCA to form a quantum dot array, and coating the quantum dot array on the surface of the P-type silicon wafer. The preparation method comprises the following steps: activating in a solution containing copper sulfate and trisodium citrate under the assistance of ultrasonic waves, carrying out chemical copper plating in a micro-fluidic reaction cavity by using an alkaline plating solution containing copper sulfate, disodium ethylene diamine tetraacetate and glyoxylic acid under the assistance of ultrasonic waves and lasers, and finally washing with deionized water and ethanol and drying with nitrogen to obtain a silicon wafer with a copper-plated surface. Through the BaTiO3 quantum dots, the micro-fluidic channel, the pulse liquid supply system and the auxiliary synergistic effect of the ultrasonic wave and the laser array, the copper film which is uniform in thickness, high in adhesive force, good in conductivity and excellent in corrosion resistance can be prepared, and the chemical copper plating efficiency and quality are improved.

The invention discloses a microfluidic and SPR battery water state identification method, equipment and a medium, and relates to the technical field of fuel cells. The method comprises the steps that the hollow optical fiber is embedded into a bipolar plate runner of the fuel cell, a cavity of the hollow optical fiber is a micro-fluidic channel, and the micro-fluidic channel is used for inflow and outflow of liquid in the fuel cell; transmitting the optical signal to the hollow optical fiber, and coating the inner wall of the cavity with a metal layer which is used for exciting SPR (Surface Plasmon Resonance); collecting a spectral signal after interaction of the liquid and the optical signal in real time through a microfluidic channel, and generating a two-dimensional spectrogram; carrying out dimensionality reduction on the two-dimensional spectrogram, extracting gray level image features, and generating a one-dimensional feature vector; and inputting the one-dimensional feature vector into a pre-trained classifier, classifying the state of the fuel cell through supervised and unsupervised mixed learning, and outputting a diagnosis result. According to the method, the state of the liquid in the battery can be accurately diagnosed in real time.

The utility model relates to a centrifugal separation tube, in particular to a microfluidic separation structure, a multi-channel separation tube and a combined type separation tube, the microfluidic separation structure comprises a body and a cavity which is arranged on the body and can contain liquid, at least one microfluidic channel is arranged on the body and located on the outer side of the cavity, and the multi-channel separation tube is arranged on the body. The inlet end of each micro-fluidic channel is communicated with the cavity, and when the separation structure works centrifugally, liquid in the cavity flows into the micro-fluidic channels from the inlet ends under the siphon action and then flows out from the outlet ends of the micro-fluidic channels. According to the separation structure disclosed by the utility model, the separation of layer liquid is realized by adopting the micro-fluidic channel, manual extraction is not needed, and the separation efficiency is improved on the basis of ensuring the separation and purification effects. Meanwhile, a multi-channel separation tube is provided by arranging a plurality of micro-fluidic channels, multi-channel separation can be carried out on layer liquid at the same time, and the separation efficiency is further improved.

The present disclosure generally relates to microfluidics, and to systems and methods for controlling the introduction of fluids. For example, certain aspects are generally directed to microfluidic devices having ports able to direct the end of a pipette tip into an end portion that is sized so as to allow fluid to flow from the pipette tip into an exit fluidly connected to a microfluidic channel. For example, the port may have a tapered portion that directs the pipette tip to the end portion. The end portion may be sized such that it is difficult for fluid to backflush around the pipette tip, and thus, the fluid is able to flow into microfluidic channels within the device, e.g., without resulting in excessive fluid remaining within the end portion. Other aspects are generally directed to methods of making or using such microfluidic devices, kits including such microfluidic devices, and the like.

The utility model discloses a PCR (Polymerase Chain Reaction) microfluidic consumable, relates to the technical field of detection consumables for pathogens, gene mutation and gene polymorphism, and aims to solve the problems that the existing PCR consumable has limitation on multiple target spot detection operations in the same batch and the overall practicability needs to be improved. Two groups of micro-fluidic channels are arranged on the outer surface of the PCR main body, each group of micro-fluidic channels comprises a storage cavity, a flow channel is arranged at one end of each storage cavity, a plurality of sample introduction holes are formed in two symmetrical sides of each flow channel, a micro-reaction chamber is arranged at one end of each sample introduction hole, one end of each flow channel is communicated with the inside of each storage cavity, and the other end of each flow channel is communicated with the inside of each sample introduction hole. One end of the sample injection hole is communicated with the interior of the flow channel, the other end of the sample injection hole is communicated with the interior of the micro reaction chamber, the cross section of the flow channel is trapezoidal, and the cross section of the sample injection hole is trapezoidal. And the effects that multiple target spot detection operations can be carried out in the same batch, and the practicability is high are achieved.

The invention relates to a microfluidic component used for electrical impedance measurement through a biological object (O), said microfluidic component comprising: A support in which is made a main microfluidic channel (C_1) defining a path for the circulation of a fluid containing the biological object (O), A trapping device positioned in the main microfluidic channel (C_1) on the circulation path of said biological object, At least one first electrode and a second electrode intended to be each set at a distinct electrical potential, in order to perform said impedance measurement, Said trapping device being composed of at least one first central pillar (P_1) and a second central pillar (P_2) positioned inside the microfluidic channel, The first central pillar being configured to form the first electrode and the second central pillar being configured to form the second electrode.Figure to be published with the abbreviation: Figure 2.