255 results about "Microfluidic channel" patented technology

The invention belongs to the technical field of medical detection, and discloses a blood disease high-throughput detection method based on a micro-fluidic chip and SERS (Surface Enhanced Raman Scattering) detection, which comprises the following steps: introducing a blood sample into the micro-fluidic chip with a micro-fluidic channel; the method comprises the following steps: performing SERS (Surface Enhanced Raman Scattering) detection on a blood sample through a nano-structure surface preset in a micro-fluidic channel; according to the present invention, with the microfluidic chip and the SERS technology, the high-throughput detection of the blood sample is achieved, the SERS spectrum data is collected and analyzed, the disease marker in the blood is identified, and the high-throughput detection of the blood sample is achieved through the combination of the microfluidic chip and the SERS technology. A plurality of micro-channels on the micro-fluidic chip can process a plurality of samples in parallel, the detection efficiency is remarkably improved, and the strength of Raman scattering signals can be remarkably improved by utilizing the surface enhancement effect of the SERS technology, so that high-sensitivity detection of blood disease markers is realized, and the detection cost is reduced. Accurate control and efficient separation of trace samples are achieved through the micro-fluidic chip, complex pretreatment steps are not needed, and the operation process is simplified.

A method for constructing a microfluidic bone organoid-on-chip can continuously observe the impacts of different external factors on a bone tissue structure based on the “ternary regulation theory” of bone angiogenesis-bone resorption-osteogenesis coupling. The method includes: (1) building a visual three-dimensional cell cultivation platform to continuously observe impacts of different external factors on a ternary regulation theory based on angiogenesis of osteoclast precursors, osteoblasts and osteoclasts, such that a user is allowed to directly observe interactions among various kinds of cells; (2) designing microfluidic channels in a chip to allow a reagent involved to present a concentration gradient distribution in the chip; and (3) adding three bioinks including osteoblasts, osteoclasts and vascular endothelial cells respectively, so as to reflect the three cell components individually, in pairs, and as a whole in the chip. An apparatus for constructing a microfluidic bone organoid-on-chip is further provided.

The embodiment of the invention discloses a multifunctional composite neural electrode system which comprises a supporting structure, a micro-fluidic structure and a flexible electrode structure, the micro-fluidic structure comprises a micro-fluidic channel; a groove is formed in the supporting structure, and at least part of the microfluidic channel is arranged in the groove; the flexible electrode structure is located on the surface of the supporting structure and covers or wraps part of the micro-fluidic channel. By adopting the technical scheme, drug delivery is performed to a target position of a nervous system through the microfluidic structure, electrical recording and chemical regulation and control can be performed on neurons at the same time, and the regulation and control effect of drugs on the neurons can be fed back in real time through electric signals.

The present invention relates to a microfluidic device (1) for forming a cell aggregate comprising at least one first cell (C1) and one second cell (C2), and for individually processing selected cells of the cell aggregate: - Microfluidic channel (10); - At least one main inlet (11) for fluids containing a first cell, a second cell, and a third cell, respectively, located in the first portion (101) of the microfluidic channel; - An outlet (12) located in the second portion (102) of the microfluidic channel for controlling the flow rate of fluid within the microfluidic channel; - A first auxiliary inlet (131) for at least one first auxiliary fluid, located in the first portion (101) of the microfluidic channel upstream or downstream of the main inlet (11); - At least one cell trapping section (14) positioned between the first and second parts within the microfluidic channel; - At least one first valve to control the flow rate of the first auxiliary fluid, causing the fluid containing the first cells and the second cells to flow at a predetermined height within the microfluidic channel, thereby guiding the first cells and the second cells to the first and second capture units, respectively. Equipped with, The microfluidic device relates to a microfluidic device in which each cell capture section (14) comprises at least one first capture section (141) and one second capture section (142), each first and second capture section being sized to accommodate a first or second cell, the first and second capture sections being adjacent to each other in a direction perpendicular to the bottom (100) of the microfluidic channel, and forming a cell aggregate containing the captured first and second cells, each cell being at a different height relative to the bottom of the microfluidic channel.

The invention relates to the technical field of biomedical engineering, in particular to a separated type organ-like chip model and a use method thereof.The model comprises a chip, an organ-like unit module and a fluid control system, and the use method comprises the steps that firstly, the chip is assembled and prepared; step 2, cell inoculation and culture; step 3, establishing and operating a fluid circulation system; step 4, organ-like unit function detection and data acquisition; 5, analyzing an experimental result and adjusting the model; the model is connected with a micro-fluidic channel through an independent cavity, and the characteristics, cell types and culture conditions of a cell culture bracket can be regulated and controlled according to the characteristics of simulated organs. Meanwhile, the micro-fluidic channel ensures stable and ordered material exchange among the organoid units, and is beneficial to more accurately screening out effective treatment schemes and drug targets.

The invention relates to a hundred-nanoscale particle sorting control chip and device and a sorting control method, the chip comprises a micro lens, a micro-fluidic channel, a sorting outlet and a sorting inlet, the sorting outlet is a two-fork opening, and the sorting inlet is a three-fork opening and comprises a sample inflow opening and two focusing inflow openings; the sorting inlets and the sorting outlets are formed in the two ends of the micro-fluidic channel respectively, and the micro-lens is arranged at the intersection of the two sorting outlets. The device comprises a chip, a light source, an injection device and an observation platform, light emitted by the light source penetrates through a micro lens and irradiates into a micro-fluidic channel, light force is applied to particles in fluid to be sorted, the particles of different sizes enter different sorting outlets, and finally sorting is achieved. Compared with the prior art, the particle sorting device can realize quick sorting of particles with the diameter of hundreds of nanometers, has the advantages of high sorting efficiency, high control precision and the like, and has important application value in sorting and control of micro-nano-scale particles such as viruses, bacteria and the like.

The invention discloses a plugging device pipe air tightness testing method and system, and relates to the technical field of air tightness test.The plugging device pipe air tightness testing method comprises the steps that a micro-fluidic channel is designed according to a structural assembly of a plugging pipeline of a plugging device, a sensor array is introduced according to detection requirements, and a micro-fluidic chip is constructed; butting the micro-fluidic chip and the plugging pipeline, and generating a parallel test task according to a pipeline assembly mode-inflation condition of the plugging pipeline; performing an inflation test on a plugging pipeline of the plugging device according to the parallel test task, when an airflow balance stage is reached, activating the sensor array to perform directional detection, determining sensing data in the microfluidic channel, and executing data fitting to determine a ventilation circulation state; and according to the ventilation circulation state, evaluating and determining air tightness data. The technical problem that in the prior art, an airtight leakage assembly cannot be accurately identified under different pipeline assembly modes and inflation conditions is solved, and the technical effects of accurately evaluating airtight data and positioning the leakage assembly under various assembly and inflation states are achieved.

The invention discloses a microfluidic sampling device capable of quickly and accurately positioning a liver cancer tumor biomarker, and relates to the technical field of medical instruments, the microfluidic sampling device comprises a chip mounting part, the chip mounting part is formed by assembling a bottom assembly disc, a microfluidic chip, a top plate and an assembly rod, and the microfluidic chip is arranged at the upper part of the bottom assembly disc; through accurate cooperation of the air pressure push rod and an external control system and flow adjustment of a valve control hole in the micro-fluidic chip, it is ensured that liquid inlet conditions for each detection are highly consistent, the detection stability is greatly improved, waste liquid generated in the detection process flows into a waste bin through a sample outlet micro-fluidic channel and a liquid outlet micro-fluidic channel, and the detection efficiency is improved. Due to the fact that the waste bin is independently arranged and is effectively isolated from untreated sample and reagent space, residual pollutants in waste liquid cannot diffuse or leak to other areas, cross contamination between samples is avoided, and specificity and sensitivity of liver cancer tumor biomarker detection are improved.

An apparatus may include a microfluidic chip including at least one microfluidic channel and a first at least one electrical interconnect. An apparatus may include a circuit board having at least one interface interconnect and a second at least one electrical interconnect electrically coupled with the first at least one electrical interconnect of the microfluidic chip. An apparatus may include a cartridge enclosing the microfluidic chip and the circuit board, the cartridge including at least one opening allowing access to the at least one microfluidic channel on the microfluidic chip and the at least one interface interconnect.

The invention relates to a blood rheological parameter online monitoring and thrombus risk assessment method, which comprises the following steps: introducing a blood sample into a microfluidic channel with a periodic geometric mutation structure, applying unsteady shear disturbance through a piezoelectric oscillation device, inducing blood to enter a non-equilibrium rheological state, and exciting a microcosmic coagulation tendency; the red blood cell aggregation behavior, the cell layer slippage phenomenon and the plasma shearing stability are recognized through the photo-thermal interference technology, and the rheological structure layer differentiation characteristics in blood are obtained; the blood subjected to shear disturbance is guided to a micro-channel high-pressure area provided with a flow-limiting threshold structure, and pressure mutation and microcoacervation nucleation generation behaviors induced by local stress disturbance are detected through a nanoscale piezoresistive sensor so as to identify sub-phase change response characteristics of the blood; and constructing a rheological instability index system on the basis of physical response signals obtained by shear disturbance, slippage and variable response, and when linkage abnormity of any two indexes is detected, judging that a high-risk event of thrombosis occurs and outputting a risk prompt signal.

The embodiment of the invention provides an encapsulated cell droplet sorting method and system, electronic equipment and a storage medium, and belongs to the technical field of intelligent biomedical engineering. The method comprises the following steps: acquiring a sorting area image of a micro-fluidic channel in a micro-fluidic chip; cutting the sorting area image according to a preset cutting frame to obtain a microfluidic droplet image; the microfluidic liquid drop image is input into a cell recognition model based on a convolutional neural network structure to obtain a cell recognition result, and the cell recognition result is used for judging whether cells exist in liquid drops or not; and determining a chip control signal of the micro-fluidic chip according to the cell recognition result so as to collect encapsulated cell droplets in the sorting area. According to the invention, the droplet cell encapsulation efficiency can be improved.

The invention belongs to the technical field of organ chips, provides an in-vitro brain lymphatic system chip construction method and a brain lymphatic system chip, and makes up the blank of brain lymphatic system in-vitro model construction. The method comprises the following steps: firstly, carrying out flushing treatment, disinfection and sterilization treatment, rinsing treatment and cell culture on a micro-fluidic channel in the chip; then, in the treated micro-fluidic channel, blood vessel channel cell inoculation and brain parenchyma channel cell inoculation are carried out; and finally, in the blood vessel channel, perfusion is carried out once at a preset flow speed every preset duration, shear force stimulation simulating pulse type blood flow is provided, basic formation of in-vitro cerebral vessels is achieved after continuous perfusion, perfusion is carried out once at a preset flow speed every preset duration, shear force stimulation simulating pulse type blood flow is provided, and in-vitro cerebral vessels are formed. Intermittent liquid flow simulating arterial fluctuation is achieved.

The invention discloses a microfluidic sorting chip and method based on the synergistic effect of multistage surface acoustic waves and sheath flow. The chip comprises a piezoelectric substrate; the PDMS micro-fluidic channel comprises a sample inlet, a sheath flow inlet, a sorting main flow channel for sorting microorganisms, and a collecting unit arranged at the tail end of the sorting main flow channel; the sheath flow inlet is converged from two sides of the sorting main flow channel through two flow paths, and forms hydrodynamic focusing with sample liquid conveyed by the sample inlet in the sorting main flow channel; the number of the multi-stage IDT arrays is four, every two of the multi-stage IDT arrays are symmetrically distributed along the two sides of the sorting main flow channel, the working frequency of the multi-stage IDT arrays is 100-150MHz, and the multi-stage IDT arrays are used for generating a superposition sound field with high energy density. According to the invention, four groups of high-frequency IDT arrays are used for generating a superposed sound field, and a PDMS sorting channel and a double-channel sheath flow system are combined, so that the sorting problem of microorganisms with acoustic contrast factors smaller than 0 is solved.

The invention discloses a chemical plating method for plating copper on the surface of a silicon wafer, which belongs to the technical field of semiconductor coating, and comprises the following steps: modifying a semiconductor material BaTiO3 nanocrystal by a silane coupling agent to prepare quantum dot ink, spin-coating the quantum dot ink on the surface of a P-type silicon wafer cleaned by RCA to form a quantum dot array, and coating the quantum dot array on the surface of the P-type silicon wafer. The preparation method comprises the following steps: activating in a solution containing copper sulfate and trisodium citrate under the assistance of ultrasonic waves, carrying out chemical copper plating in a micro-fluidic reaction cavity by using an alkaline plating solution containing copper sulfate, disodium ethylene diamine tetraacetate and glyoxylic acid under the assistance of ultrasonic waves and lasers, and finally washing with deionized water and ethanol and drying with nitrogen to obtain a silicon wafer with a copper-plated surface. Through the BaTiO3 quantum dots, the micro-fluidic channel, the pulse liquid supply system and the auxiliary synergistic effect of the ultrasonic wave and the laser array, the copper film which is uniform in thickness, high in adhesive force, good in conductivity and excellent in corrosion resistance can be prepared, and the chemical copper plating efficiency and quality are improved.

The invention discloses a microfluidic and SPR battery water state identification method, equipment and a medium, and relates to the technical field of fuel cells. The method comprises the steps that the hollow optical fiber is embedded into a bipolar plate runner of the fuel cell, a cavity of the hollow optical fiber is a micro-fluidic channel, and the micro-fluidic channel is used for inflow and outflow of liquid in the fuel cell; transmitting the optical signal to the hollow optical fiber, and coating the inner wall of the cavity with a metal layer which is used for exciting SPR (Surface Plasmon Resonance); collecting a spectral signal after interaction of the liquid and the optical signal in real time through a microfluidic channel, and generating a two-dimensional spectrogram; carrying out dimensionality reduction on the two-dimensional spectrogram, extracting gray level image features, and generating a one-dimensional feature vector; and inputting the one-dimensional feature vector into a pre-trained classifier, classifying the state of the fuel cell through supervised and unsupervised mixed learning, and outputting a diagnosis result. According to the method, the state of the liquid in the battery can be accurately diagnosed in real time.

The present disclosure generally relates to microfluidics, and to systems and methods for controlling the introduction of fluids. For example, certain aspects are generally directed to microfluidic devices having ports able to direct the end of a pipette tip into an end portion that is sized so as to allow fluid to flow from the pipette tip into an exit fluidly connected to a microfluidic channel. For example, the port may have a tapered portion that directs the pipette tip to the end portion. The end portion may be sized such that it is difficult for fluid to backflush around the pipette tip, and thus, the fluid is able to flow into microfluidic channels within the device, e.g., without resulting in excessive fluid remaining within the end portion. Other aspects are generally directed to methods of making or using such microfluidic devices, kits including such microfluidic devices, and the like.

The invention relates to a microfluidic component used for electrical impedance measurement through a biological object (O), said microfluidic component comprising: A support in which is made a main microfluidic channel (C_1) defining a path for the circulation of a fluid containing the biological object (O), A trapping device positioned in the main microfluidic channel (C_1) on the circulation path of said biological object, At least one first electrode and a second electrode intended to be each set at a distinct electrical potential, in order to perform said impedance measurement, Said trapping device being composed of at least one first central pillar (P_1) and a second central pillar (P_2) positioned inside the microfluidic channel, The first central pillar being configured to form the first electrode and the second central pillar being configured to form the second electrode.Figure to be published with the abbreviation: Figure 2.

The invention relates to a high-sensitivity differential microfluid sensor based on a microstrip line, which is used for measuring the concentration of a water-ethanol mixed solution, and comprises the microstrip line configured with a symmetrical shunt / combiner, a pair of split ring resonators with bent grooves, and a microfluid channel, a dielectric layer and a metal ground which are arranged at the tops of the split ring resonators from the top layer to the bottom layer. Wherein the bent groove parts of the two SRRs are areas with the maximum electric field intensity, and a micro-fluidic channel made of polydimethylsiloxane (PDMS) is placed in the areas. A sensor parameter curve is measured by a vector network analyzer (VNA). The suggested size of the sensor is 30 * 20 mm < 2 >, and the working frequency is 4.8 GHz. Experimental results show that the resonant frequency of the water-ethanol mixture with the concentration range of 0-100% has linear shift of 1250 MHz, and the average sensitivity Savf of detection is 0.987%. The sensor has the characteristics of compactness, high sensitivity, high measurement precision, no environmental interference and the like, and has wide application prospects in the fields of biomedical detection, material analysis, environmental monitoring and the like.

A microfluidic system for controlling movement of charged particles, including a microfluidic channel, at least three electrodes, a plurality of conductor leads and at least two drive power supplies. Each electrode is in electrical contact with the electrolyte, and a pseudo-capacitance, a double-layer capacitance or a combination thereof is formed at an electrode-electrolyte interface. Each drive power supply periodically produces a voltage or current excitation, and is connected to one or more electrodes via the conductor leads. Each electrode continuously undergoes charging-discharging alternating cycles to form a traveling-wave electric field in the microfluidic channel. A maximum charge capacity of each electrode is greater than a total amount of charges transferred thereon during a charging or discharging process. A control method based on the microfluidic system is also provided.

The application discloses a hemolysis analyzer based on an integrated optical waveguide sensor and a preparation method thereof, and comprises an integrated photon waveguide, a nano filter and a micro-fluidic channel, wherein the integrated photon waveguide is a single waveguide with a middle spiral type and coupling gratings on both sides. The hemolysis analyzer fully utilizes the advantages of high sensitivity and small volume of the integrated optical waveguide sensor, and realizes sample-free preparation by combining the methods of instantaneous absorption and hemoglobin filtration, and can quickly and accurately detect hemolysis and the degree of hemolysis.

The invention relates to the technical field of collagen intelligent monitoring, and particularly discloses an intelligent collagen monitoring method and system integrated with a microsensor. Enzyme response hydrogel with switchable states is anchored to the skin through a microneedle array, a high-frequency acoustic impedance analysis module is used for continuously probing the acoustic impedance change of the hydrogel, and the collagen monitoring accuracy is improved. Identifying a collagen degradation event; once collagen degradation is detected, the system activates the micro-regeneration unit, a cleaning solution and a regeneration solution are conveyed through a microfluidic channel, a broken peptide chain is removed, a hydrogel cross-linked network is reconstructed, and functional regeneration of the sensing unit is realized; the system further comprises a regeneration state confirmation and cycle reset mechanism, and the reliability of each monitoring cycle and the consistency of data references are ensured. According to the method, the limitation of a traditional monitoring method is overcome, multiple times of recycling of a single sensor is achieved, the credibility and consistency of long-term monitoring data are improved, and the method has important significance on skin health management and senescence research.

The utility model relates to the technical field of endotoxin monitoring, in particular to an endotoxin detection system. The utility model aims to solve the problem that the detection efficiency of the creotoxin is low. The endotoxin detection system comprises a light source emitting device, an incident light lead, a microfluidic channel, an optical fiber biochemical sensor, an emergent light lead and a spectral analysis device, the optical fiber biochemical sensor is arranged inside the microfluidic channel, the optical fiber biochemical sensor is provided with a sample groove, the microfluidic channel is provided with at least two sample inlets and at least one sample outlet, and a microfluidic channel area between any sample inlet and any sample outlet covers the sample groove; one end of the incident light lead is connected with the light source generating device, the other end of the incident light lead penetrates into the micro-fluidic channel and is connected to the optical fiber biochemical sensor, one end of the emergent light lead is connected with the optical fiber biochemical sensor, and the other end of the emergent light lead penetrates out of the micro-fluidic channel and is connected to the spectral analysis device.

The invention belongs to the technical field of kidney disease detection, and particularly relates to a renal function dry-type biochemical detection chip and a use method thereof. The chip is combined with a dry biochemical reaction layer through a micro-fluidic technology, and can be used for synchronously detecting renal function markers such as urine creatinine, urea nitrogen and cystatin C. The chip adopts a multi-layer modular lamination design, and sequentially comprises a bottom plate, an electrode layer, a detection layer, a binding layer and a sample adding layer from bottom to top, and each layer is made of a high-molecular polymer with excellent biocompatibility, so that the detection process is not interfered, and the result is accurate and reliable. Sample flow is accurately controlled through the microfluidic channel, multi-index parallel detection is realized, and the detection efficiency is improved. A micro sensor is arranged in the chip, the reaction process is monitored in real time, and data accuracy is ensured. The system has the characteristics of portability and quick response, is suitable for clinical and family self-test, and provides a convenient and efficient solution for renal function monitoring.

The invention discloses an octopus bionic sucker flexible negative voltage electrode patch for nerve electrical regulation and control. The octopus bionic sucker flexible negative voltage electrode patch comprises a flexible substrate, an octopus bionic sucker array and a microneedle electrode array, a patterned micro-fluidic channel is arranged in the flexible substrate, and a first micro-fluidic hole array is arranged on the surface of the flexible substrate; the octopus bionic suction cup array has flexibility and adhesion and comprises a suction cup array, an electrode hole array arranged on the suction cup array and a second micro-fluidic hole array, and the second micro-fluidic hole array and the first micro-fluidic hole array correspondingly form a micropore channel; the microneedle electrode array is inserted into the electrode hole array; when the electrode patch is used for nerve electric regulation and control, under the action of pre-tightening force, the suction cup adheres to the skin and is preliminarily attached to the skin, the micro-needle electrode array on the suction cup acts on the cortex, gas in the suction cup is extracted through the micro-fluidic channel and the micropore channel to obtain an external negative pressure source, stable adsorption force is provided through negative pressure, sweat on the surface of the human body is discharged, and therefore the skin can be absorbed. And long-term high-quality nerve electric regulation and control are realized.

The application provides a method for synthesizing benzimidazole Michael addition derivatives in a continuous flow reactor, and 2-methylbenzimidazole and alpha, beta-unsaturated olefins are used to catalyze the online synthesis of benzimidazole Michael addition derivatives in a microfluidic channel reactor by using a lipase Lipozyme RM IM. The method not only greatly shortens the reaction time, but also has high conversion rate; meanwhile, the Michael addition reaction of 2-methylbenzimidazole and alpha, beta-unsaturated olefins is catalyzed by using the economical lipase Lipozyme RM IM in methanol, the reaction cost is reduced, the post-processing step is simplified, and the method has the advantages of green environmental protection, economy and efficiency.

The utility model discloses a sperm optimization device, belongs to the technical field of assisted reproduction, and solves the problems that the sperm quality is reduced and the sperm optimization efficiency and the assisted reproduction success rate are influenced due to the fact that the micro-fluidic channels are too few and the high-quality sperm enrichment time is too long in the prior art. The device comprises a lower plate body and an upper plate body, the upper plate body is fixed on the upper surface of the lower plate body, and the upper surface of the lower plate body is provided with an inlet pool, an annular groove, a screening channel and an outlet pool; the two symmetrical annular grooves are formed in the two sides of the lower plate body, the inlet pool is communicated with the annular grooves, the outlet pool is arranged in the circular center of the annular grooves, and the outlet pool is communicated with the annular grooves through the screening channel. According to the sperm screening device, high-quality sperms are screened by utilizing the sperm activity and the boundary navigation function, the speed of a buffer solution does not need to be controlled, the screening success rate is ensured, the operation is simple, and the learning cost is low.

The present application relates to a kind of hundred nanometer level particle sorting manipulation chip, device and sorting manipulation method, the chip includes microlens, microfluidic channel, sorting outlet and sorting inlet, the sorting outlet is bifurcation, the sorting inlet is trifurcation, including sample flow inlet and 2 focus flow inlets;Sorting inlet and sorting outlet are respectively arranged at the two ends of microfluidic channel, and microlens is arranged at the intersection of 2 sorting outlets.The device includes chip, light source, injection device and observation platform, and the light emitted by light source is irradiated into microfluidic channel through microlens, and light force is applied to the particle in the fluid to be sorted, and different size particles enter different sorting outlets, finally realize sorting.Compared with prior art, the present application can realize the rapid sorting of hundred nanometer level particle, with the advantages of high sorting efficiency, high control precision, etc., and has important application value for the sorting and manipulation of micro-nano scale particles such as virus and bacteria.

This invention relates to the field of biomedical technology, specifically to a microfluidic channel, a microfluidic chip, and their applications for squeezing cells. The microfluidic channel is composed of several continuously arranged cell squeezing units. Each cell squeezing unit consists of a main channel and a squeezing region. The main channel is composed of connecting sections and curved sections. The squeezing region includes a first squeezing section, a squeezing slit, and a second squeezing section. The width of the first squeezing section gradually decreases from the main channel, reaching its minimum at the squeezing slit, and then gradually increases towards the second squeezing section until it matches the width of the main channel. The squeezing slit is rectangular, with a width of 4-10 μm and a length of 20-60 μm. The microfluidic channel provided by this invention applies relatively uniform and dispersed mechanical stress to the cell membrane, reducing cell breakage and mortality rates, and allowing cells to restore their integrity through the cell membrane's self-repair mechanism after squeezing.

Three-dimensionally (3D) printed, wearable, electronic sensing articles and methods of manufacture are disclosed. In one example embodiment, the present disclosure provides A wearable article that includes a three-dimensionally printed base, wherein the base of the article comprises a three-dimensionally printed mesh which is formed of an elastomeric polymer composition. The wearable article also includes a microfluidic channel coupled to the mesh, the microfluidic channel having an inlet and an outlet disposed at opposite ends of the channel, wherein the microfluidic channel is curved in a form of a serpentine shape; and wherein the serpentine shape is provided by a plurality of 180-degree semi-circular segments.

The application provides an electrochemical sensing ELISA reagent card, comprising a microfluidic channel body and an electrochemical sensor body which are bonded together, wherein: a plurality of "annular" liquid flow channels are arranged on the microfluidic channel body, each "annular" liquid flow channel is formed by a working electrode covering flow channel and a counter electrode and a reference electrode covering flow channel in series, and the "annular" liquid flow channels are communicated through a common flow channel; the electrochemical sensor body comprises a flat plate substrate and a plurality of screen-printed electrodes which are printed in parallel on the flat plate substrate, and each screen-printed electrode comprises a working electrode, a counter electrode and a reference electrode. The electrochemical sensing ELISA reagent card combines an electrochemical biosensor and a microfluidic chip, and solves the technical problems of large sample / reagent consumption, single detection index and liquid volume influence on absorbance measurement of a traditional ELISA reagent kit.