The invention discloses a prothallus proliferation culture medium and a rapid propagation method for rare and endangered
plant cibotium barometz. The prothallus proliferation culture medium of
cibotium barometz is used for accelerating the proliferation of gametophytes, and the prothallus maturation time is shortened; meanwhile, the invention provides a rapid propagation method of
cibotium barometz, which comprises the following steps: carrying out artificial sterile culture on
cibotium barometz spores, rapidly germinating in a culture
bottle to form prothalli, culturing on a prothalli proliferation culture medium, inducing and transforming gametophytes in a specific matrix outside the
bottle to form sporophytes by virtue of an incomplete
tissue culture method, and
transplanting. According to the method, the formation of prothallium is accelerated, the
sporophyte conversion rate is increased, the
reproduction rate is increased while the
domestication and
reproduction of
sporophyte seedlings are accelerated, a large number of
cibotium barometz seedlings which grow healthily can be obtained, the
survival rate reaches 90%, the
spore breeding period is obviously shortened, the operation requirement is not strict, and the method is suitable for large-scale popularization and application. The technical problems that the seedlings in the
tissue culture bottle are easy to pollute, fragile and low in
seedling rate are solved.