31 results about "Sporophyte" patented technology

The invention discloses a cibotium barometz sporophyte transformation method and system based on multi-factor coupling regulation, and relates to the technical field of biology. The method comprises the following steps: synchronously acquiring environment data and sporophyte image data; preprocessing the environment data, performing feature extraction on the image data, and splicing the processed data into a comprehensive feature vector; quantifying the weight, correlation degree and interaction level among the environmental factors through an entropy method, grey correlation analysis and a coupling coordination degree model; constructing an improved CNN-LSTM neural network, extracting features through a double-branch structure, fusing a coupling analysis result to carry out cross-modal fusion, and training to obtain a prediction model; when the predicted conversion rate is lower than a threshold value, environment parameters are automatically adjusted until conversion is completed; according to the method, the problems of environmental factor data isolation and regulation lag in the prior art are solved, accurate prediction and intelligent regulation of the sporophyte transformation process are realized, and the transformation success rate and the production efficiency are remarkably improved.

The invention discloses a method for cultivating triploid kelp sporophytes and identifying ploidy of induced protonema, and belongs to the field of kelp breeding. The method is based on a capillary glass needle separation technology for inducing filamentous somatic cells by kelp and a molecular biological technology for specific marking of sex of kelp gametophytes. The sex specific marker of the kelp gametophyte is used for carrying out PCR (Polymerase Chain Reaction) amplification and 1.5% agarose gel electrophoresis detection on the separated induction protonema single-cell cloning line, so that the genetic sex of the kelp induction protonema can be accurately identified; and the ploidy of the induced protonema of a part of varieties (lines) is directly determined from the molecular level. The method for synchronously detecting the genetic sex and ploidy of the induced protonema breaks through the bottlenecks of chromosome counting and flow cytometry operation, can be completed only through one-time PCR amplification, and is simple, convenient, rapid, short in time consumption, capable of completing detection of a plurality of samples at the same time and low in cost.

This invention provides the nucleotide and amino acid sequences of the Dip gene, as well as its (functional) homologs, fragments, and variants, which provide ploid sporophyte formation as part of apomixis. It also provides ploid sporophyte-forming plants, methods for their preparation, methods for using them, and methods for preparing apomixis seeds.

The invention relates to genetic breeding, in particular to a method for cultivating kelp triploid by inducing a monosexual dihaploid (DH) gametophyte. The method specifically comprises the following steps: establishing a kelp DH sporophyte culture system, inducing DH sporophytes to generate unisexual DH gametophytes (2n) through spore-free propagation, and then hybridizing the unisexual DH gametophytes (2n) with haploid (n) gametophytes with opposite sex to obtain kelp triploid (3n) sporophytes. And determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometry, thereby identifying the chromosome ploidy of the hybrid sporophyte as 3n. The method can be used for cultivating new varieties of high-yield, high-quality, stress-resistant and sterile kelp, and has important significance for developing genetic breeding and new variety creation research in kelp and even kelp brown algae.

The application discloses a method for regenerating direct organs of Cyatheasporophyte leaves, and relates to the field of in-vitro rapid propagation technology.The method induces spores from Cyatheasporophyte protoleaves, then induces green globular bodies (GGBs) from the spores, and after the GGBs germinate into new spores, the new spores are used to induce spore leaf clusters through a leaf direct organ pathway, and the regenerated spore leaf clusters are subcultured and rooted to form complete plants.The method provided in the application has a high spore propagation coefficient and a short cycle through the spore leaf direct organ pathway.

This invention relates to a method for the germination of Cibotium barometz spores and a method for the propagation of Cibotium barometz, belonging to the field of plant culture technology. The propagation method of this invention includes the following steps: disinfection: disinfecting Cibotium barometz spores with a 10% sodium hypochlorite solution for 5-10 minutes; spore germination: the germination medium used includes 1 / 4 MS medium, 4.5-7 g·L⁻¹. ‑1 Agar and 0-30 g·L ‑1 Sucrose; Sporophyte differentiation: The sporophyte differentiation media used included 1 / 2 MS medium and 0-1 mg / L... ‑1 6-BA, 0.5-1 mg·L ‑1 2,4-D, 4.5-7 g·L ‑1 Agar and 25-30 g·L ‑1 Sucrose; Sporophyte strengthening: The sporophyte strengthening medium used included 1 / 4 MS medium, 4.5–7 g·L⁻¹. ‑1 Agar, 30 g·L ‑1 Sucrose and 1-2 g·L ‑1 Activated carbon; hardening and transplanting: After the tissue culture seedlings are sealed, the seal is gradually lifted, and they are then transferred to a specific mixed substrate for cultivation to obtain commercial seedlings. This method can improve the spore germination rate by using a specific sterilization procedure, and achieves the effect of producing more and better seedlings by using specific culture media and hardening preparations at each stage.

The application discloses a kind of Sargassum fusiforme fertilized egg suspension culture new germplasm creation and evaluation method, including wild Sargassum fusiforme geographical parent selection, isolation breeding and unit mass evaluation;Parent high-value character strain female and male plant screening, isolation breeding and enemy biological control;High-value character strain female and male plant epiphytic enemy biological removal;High-value character strain female and male plant lateral branch indoor temporary nursing optimum environmental factor creation;Parent high-value character strain female and male plant lateral branch indoor temporary nursing processing and temporary nursing time;New germplasm fertilized egg classification collection, impurity removal and cleaning, new germplasm fertilized egg suspension system creation;New germplasm embryo to young sporophyte suspension growth and development stage suspension system typical enemy biological control;New germplasm young sporophyte is clamped and indoor temporary nursing;New germplasm young sporophyte is put in sea area;Parent and offspring new germplasm evaluation method;Can be Sargassum fusiforme new germplasm creation, new germplasm quality evaluation and new variety cultivation provide scientific, standard operation and evaluation method.

This invention belongs to the field of plant molecular breeding, and specifically discloses a gene related to rice fertility restoration. OsRf19 This invention addresses the cumbersome process of identifying restorer lines for the existing Fujian abortive cytoplasmic male sterility (CMS-FA / OsRf19) system by developing a method for creating tightly linked artificial haplotypes, the development of InDel molecular markers, and their application in rice fertility. Through bioinformatics analysis, this invention identifies a fertility restoration gene associated with the rice sporophytic sterility system CMS-FA. OsRf19 A specific region closely linked to the PPR gene cluster and with extremely low similarity to the loci was edited using CRISPR / Cas12a gene editing technology, resulting in multiple large deletions. Based on these genotype sequence variations, a set of InDel molecular markers was developed to realize the editing of this artificially created restoration gene. OsRf19 Rapid identification of closely linked artificial haplotypes accelerates the breeding process of molecular marker-assisted selection in the rice CMS-FA system.

Methane production in ruminants can be reduced by improving rumen fermentation efficiency through dietary supplementation with Asparagopsis taxiformis. This disclosure relates to a method for producing A. taxiformis and its use. In a specific form, this disclosure relates to a method for producing tetrasporophytes and gametophytes of A. taxiformis by environmental manipulation.

The invention discloses a tissue culture method for botrychium ternatum spore induced prothallium. The culture method comprises the following steps: collecting botrychium ternatum with sporangium, drying, sieving and collecting spores, sequentially carrying out multiple times of disinfection and cleaning pretreatment on the collected spores by using 75% ethanol, sterile water, 2% sodium hypochlorite and the like in a sterile environment, uniformly coating a culture medium with treated spore suspension, and carrying out observation culture, the time from spore inoculation to prothallium formation is 24-26 days, and the time from spore inoculation to juvenile sporophyte formation is 47-63 days. The culture medium consists of the following components: a 1 / 8 MS culture medium, 5g / L of sucrose and 8g / L of agar. The culture medium is simple to prepare, can effectively induce spore germination and prothallus proliferation of the botrychium ternatum, can be further cultured to obtain sporophytes, can also be used for acclimatization and transplantation, shortens the seedling raising period, further realizes large-scale cultivation of the botrychium ternatum, and has important significance for expanded application of pteridophyte.

The application provides a live sowing and seedling raising method for Dryopteris filix-mas spores and belongs to the technical field of spore propagation of medicinal ferns. The live sowing and seedling raising method for Dryopteris filix-mas spores comprises the following steps: collecting Dryopteris filix-mas spores, sowing on the surface of a culture medium, spraying nutrient solution according to different growth periods, and growing into spore bodies; the culture medium is composed of humus soil, peat soil and limestone at a volume ratio of 3-5:1-3:1-2; during sowing and germination of prothallus, 1 / 8MS+0.01-0.05mg / L GA3+0.3-0.7mg / L 6-BA nutrient solution is sprayed; during the period from prothallus to spore body growth, 1 / 2MS+0.3-0.7mg / L KT+0.3-0.7mg / L 6-BA+1.5-2.5mg / L IBA nutrient solution is sprayed. The application can significantly improve the emergence speed and propagation efficiency of Dryopteris filix-mas by using a specific culture medium and supplementing suitable nutrient solution, and has important significance for realizing artificial planting and resource protection of live sowing and seedling raising of Dryopteris filix-mas.

The invention discloses a module for preparing male gametophyte sterility and a preparation method and application thereof, the module is formed by connecting a pollen specific promoter, an interference element of an OsPTDa gene and an interference element of an OsPTDb gene in series through tRNA-Gly, and the gametophyte male sterility module can be obtained after the elements are assembled. The gametophyte male sterility module disclosed by the invention can down-regulate the expression of male gamete development related genes OsPTDa and OsPTDb at the same time, so that the pollen insemination ability is lost, gametophyte male sterility is generated, and the gametophyte male sterility module plays an important role in preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

The invention discloses a gametophyte male sterility related gene OsPTDb and application thereof. The amino acid sequence of the OsPTDb gene is as shown in SEQ ID NO. 1; the OsPTDb gene encodes an ascorbic acid oxidase which is an essential gene for male gametophyte development, the insemination of the male gametophyte cannot be completed and gametophyte male sterility is generated due to the abnormal function of the gene, and the OsPTDb gene plays an important role in the aspects of preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

The present invention relates to the field of expression of recombinant glycoproteins in plants. In particular, the present invention relates to a method of producing a genetically modified plant capable of producing glycoproteins harbouring paucimannosidic N-glycans, to a method of producing a glycoprotein of interest harbouring paucimannosidic N-glycans and to genetically modified plants, plant cells, protoplasts, protonemas, gametophytes, sporophytes or spores produced with or used in such methods.

The application provides a method for artificially breeding Doerryoptis concolor. The method for artificially breeding Doerryoptis concolor comprises the following steps: (1) sowing spores of Doerryoptis concolor in a breeding substrate, and culturing until obtaining sporophyte seedlings; and (2) transplanting the sporophyte seedlings into a rejuvenation substrate, and performing division rejuvenation culture. The method has high germination rate, high survival rate, high growth quality, and fast growth speed, and can effectively meet the demand of the market for Doerryoptis concolor.

Described herein are compounds, formulations, and methods for blocking sporozoite invasion and subsequent liver-stage parasite development of a protozoan parasite, such as Plasmodium falciparum.

The invention discloses a chimeric interference pollen lethal module as well as a preparation method and application thereof. The module consists of a pollen specific promoter, a chimeric interference element of an OsPTDa gene and an OsPTDb gene, and a plant endogenous terminator. After the elements are assembled, pollen can be killed. The pollen lethal module disclosed by the invention can accurately down-regulate the expression of male gametophyte development related genes OsPTDa and OsPTDb in the same time and space, so that pollen germination is abnormal, transgenic pollen cannot generate offspring, gametophyte male sterility is generated, and the pollen lethal module has important value in the aspects of preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

Methane production in ruminant animals may be reduced by improving rumen fermentation efficiency through dietary supplementation of Asparagopsis taxiformis. The present disclosure relates to a method of producing A. taxiformis and uses thereof. In particular forms, the present disclosure relates to methods of producing a tetrasporophyte and a gametophyte of A. taxiformis through environmental manipulations.

The invention relates to germplasm preservation, propagation and seedling cultivation of undaria pinnatifida, in particular to a method for large-scale updating and rapid propagation of undaria pinnatifida gametophyte germplasm. Specifically, obtained mature homozygous double (DH) sporophytes are dried in shade and stimulated to diffuse a large number of zoospores, and the spores germinate to form gametophytes consistent with parent genotypes, so that large-scale updating and rapid propagation of gametophyte germplasm are realized. The method disclosed by the invention effectively solves the technical bottleneck that a large number of gametophyte clones are difficult to obtain in a short time due to slow cell division of gametophytes from single cells, and has important significance on creation of new undaria pinnatifida hybrid varieties and large-scale seed production.

The invention discloses a sporophyte fertile gene OsSTRL3 and application thereof. A protein encoded by the sporophyte fertile gene is an amino acid sequence as shown in SEQ ID NO.1, or a protein which is subjected to substitution, deletion or addition of at least one amino acid and has the same function as the OsSTRL3; the nucleotide sequence of an encoding region of the OsSTRL3 gene is shown as SEQ ID NO.2. The invention also discloses a mutation site of the OsSTRL3. The OsSTRL3 gene disclosed by the invention encodes a protease with an isosessile synthetase / phospholactonase structural domain, and has a function of regulating and controlling normal development of pollen, sporophytic male sterility can be generated by mutation of the gene, and the mutation of the OsSTRL3 gene can be used for regulating and controlling normal development of pollen. The strain can be used as a female parent for producing hybrids in heterosis utilization.

This invention provides a sex-specific molecular marker for kelp and its application, wherein the nucleotide sequence of the male marker fragment is SEQ ID NO:1, and the nucleotide sequence of the female marker fragment is SEQ ID NO:2. The gene sequence fragments obtained by this invention are derived from conserved homologous genes in the sex-determining regions of brown algae. The primer sequences for the selected sex identification markers are derived from the regions with the highest similarity in the coding sequences of the homologous genes. This method enables rapid identification of the sex of kelp gametophytes using only a pair of degenerate primers, offering high efficiency, high accuracy, and high interspecies compatibility. This method is applicable to the identification of mixed male and female samples, the differentiation of kelp sporophytes from parthenogenesis and sexual reproduction, and the sex identification of gametophytes in other species of the Laminariales order, providing important support for the efficient management and research utilization of kelp gametophyte germplasm banks.

A bioreactor includes a first compartment designed to retain seaweed sporophytes, a second compartment in fluid communication with the first compartment that includes one or more settlement surfaces, and a first porous barrier between the first and second compartments that allows the seaweed spores to pass from the first compartment to the second compartment, and systems comprising the bioreactor. Also provided herein are methods of culturing seaweed, for example, using a bioreactor provided herein.

The invention discloses a method for improving the induction rate of prothalli and sporophytes of cibotium barometz, which comprises the following steps: S1, sterilizing a substrate by using a bactericide which comprises thiophanate-methyl and tebuconazole, and after sterilization, reducing the water content of the substrate to 40-50% to obtain the sterilized substrate; s2, contacting the bottom of the substrate with a hormone aqueous solution to obtain a substrate absorbing the hormone aqueous solution; s3, sowing the cibotium barometz spores on the surface of the substrate for culture. The method shortens the spore germination time of the cibotium barometz, improves the prothallus and sporophyte induction rate, and is simple and easy to implement. By adopting the method to cultivate the golden dog seedlings, prothalli are induced in 22 days on average, and sporophytes are induced in 66 days on average. The method can be widely applied to sowing and seedling raising of cibotium barometz, and seedling raising time and labor cost are saved.

The invention discloses a plant pollen specific expression promoter POsPTDb and application thereof. The nucleotide of the promoter POsPTDb is as shown in SEQ ID NO. 1; the pollen specific promoter POsPTDb is derived from a promoter of a rice endogenous gene, and can specifically drive a target gene or a DNA fragment to be expressed in pollen; the promoter is used for driving specific expression of a gene or a DNA (deoxyribonucleic acid) fragment capable of controlling pollen fertility in pollen, gametophyte male sterility can be prepared, and the promoter has important value in the aspects of preventing transgenosis from drifting along with pollen, breeding sporophyte nuclear sterility and the like.

The application discloses a method for delaying spawning of reef membrane gametes, which comprises the following steps: cleaning, air-drying, sealing, storing, soaking and standing of the collected mature reef membrane gametophytes with robust appearance and without spawning gametes at room temperature, in a dense bag, at 4 DEG C and in dark conditions, in sterilized seawater with 90-900 times of the weight of the algal body, and under the conditions of temperature, humidity and light intensity.

The present disclosure discloses a method for breeding a recessive genetic male sterile line of a sporophyte. The method comprises the following steps: linking a sporophyte male fertile gene Y, a down-regulation expression element Xi of an endogenous gametophyte male fertile gene, a herbicide A resistance gene AR, a down-regulation element Bi of a herbicide B resistance gene, and an anthocyanin gene C or a down-regulation element Ci thereof, and transferring the linked genes into a sterile mutant of the sporophyte fertile gene Y, and pollinating a sterile plant with a positive plant to breed a genetic sterile line of a sporophyte. The method is universal and suitable for all plants, can obtain a genetic sterile line of a sporophyte with a sterile plant rate of 100%, reduce a cost for separating a sterile line from a maintainer line, identify purity intuitively, rapidly, accurately, and economically at an early stage.

The present application relates to an anticoccidial composition comprising ginkgo biloba leaves and uses thereof. According to embodiments, the composition comprising ginkgo biloba leaves has an excellent effect of directly killing sporozoites that can induce coccidiosis, inhibiting penetration of sporozoites into cells, and / or inhibiting proliferation of sporozoites in cells, and has an excellent effect on prevention, improvement, and treatment of coccidiosis in vivo.

This invention discloses a method for improving the induction rate of prothallus and sporophyte in *Cibotium barometz*, comprising: S1, sterilizing the substrate with a fungicide, wherein the fungicide includes thiophanate-methyl and tebuconazole, and after sterilization, reducing the substrate moisture content to 40-50%, obtaining a sterilized substrate; S2, contacting the bottom of the substrate with a hormone aqueous solution, obtaining a substrate absorbent with the hormone aqueous solution; S3, sowing *Cibotium barometz* spores on the substrate surface for cultivation. This invention shortens the spore germination time of *Cibotium barometz*, improves the induction rate of prothallus and sporophyte, and is simple and easy to implement. Using this method to cultivate *Cibotium barometz* seedlings, prothallus are induced in an average of 22 days, and sporophyte are induced in 66 days. This method can be applied on a large scale to the sowing and seedling cultivation of *Cibotium barometz*, saving seedling time and labor costs.