209 results about "Sporophyte" patented technology

The present invention relates to a method for propagation and cultivation of platycerium. It is characterized by that said invention adopts sterile spore germination technique and makes it be combined with test-tube seedling-cultivating technique to implement propagation and cultivation of platycerium. Said method includes the following several steps: collecting storing, health and mature spore, making sterile spore germination, gametophyte growth culture, insemination to form zygoite, sporophyte enrichment culture, rooting culture and transplantation, etc.

The invention discloses a gulfweed transplanting method based on a juvenile sporophyte adhesion function. The method includes the steps of firstly, collecting fertilized gulfweed archegoniophore; secondly, crushing the gulfweed archegoniophore, adding sea water, well mixing, filtering impurities, and collecting the filtered liquid mixture; thirdly, concentrating the liquid mixture with a centrifuge to prepare concentrated mucus; fourthly, well mixing juvenile sporophyte and the concentrated mucus to obtain juvenile sporophyte concentrated mucus; fifthly, diving to reach the seabed, removing organisms and sediments adhering to a seabed ledge matrix, and smearing the juvenile sporophyte concentrated mucus on the surface of the seabed ledge matrix. By the method, the problems that gulfweed juvenile sporophyte adhesion success probability is reduced due to the actions of waves and sea currents, and the juvenile sporophyte cannot be adhered successfully are solved.

The invention discloses a summering cultivation method for seedlings of Hizikia fusiform (Harv)Okamura juvenile sporophytes, comprising the following steps: raising seedlings in a north sea area at a high latitude during the high temperature stage; and transferring the cultivated seedlings to a south cultivation sea area at a low latitude after summering for cultivation. In the invention, seedling raising and cultivation are performed in different levels and sea areas, thus overcoming the disadvantageous sea conditions in cultivation sea areas, and avoiding high energy consumption in an indoor temperature-reduction cultivation mode.

The invention discloses a propagation method of cyrtomium fortune. The propagation method comprises the following processing steps of: 1, preparing a culture medium; 2, sterilizing cyrtomium fortune spores; 3, inoculating and cultivating; and 4, reculturing seedling and transplanting cyrtomium fortune sporophyte seedling. The propagation method has the following advantages that: deficiencies of long culture period, high cost and low efficiency of the conventional cyrtomium fortune propagation technology are made up, and a new way for artificial propagation of the cyrtomium fortune is developed. Few spore materials are utilized, so that a large amount of sporophytes can be quickly propagated, the seedling is formed in 65 days, and the planting percent is about 97%, so that large-scale industrial production of the cyrtomium fortune becomes possible, thus requirements of the market on the cyrtomium fortune are satisfied, and meanwhile, damage on the wild resources and the environment canbe reduced. Furthermore, the invention further provides a good testing system for developing researches about genetic breeding of species and conservation biology, and wide application prospect is provided.

The invention discloses a plug-in artificial algal reef and a method for constructing an algal field by using the same. The algal reef comprises a solid base and an attached block movably connected to the solid base through a connecting rod, wherein the attached block is semispherical and has a rough surface, thereby meeting the requirement for the attachment of algae germ cells; and both the solid base and the attached block can be formed by one-step concrete pouring. When the algal field is constructed, the solid base is put into the sea and is soaked for a period of time; meanwhile, the attached block is dipped into seawater containing algae germ cells, and the attached block subjected to species introduction is movably connected to the solid base through the connecting rod after the algae germ cells are attached to the attached block. The plug-in artificial algal reef is convenient to use and is firm and durable, and algae can be prepositively fixed on the attached block so that germ cells diffused by algae sporophytes continue growing on the solid base; the germ cells is prevented from being swallowed by natural enemies, and the success rate of algae introduction is increased; the rehabilitation of the algal field can be realized, and a foundation is laid for building a new algal field.

The invention belongs to the field of agricultural biotechnology and relates to a method for rapidly reproducing new pteris fern seedlings by using prothallium reproduction approaches. The method comprises the steps of prothallium culture process, multiplication culture process, sporophyte induction process, rooting induction process, and transplant and manage process. The technological process is simple; by reproduction in the form of prothallium, a great amount of prothallium can be obtained; by the processes of multiplication, sporophyte induction, sporophyte rooting and transplant, a great number of seedlings capable of being used for production and culture can be obtained; the obtained seedlings are at the same physiological age and grow uniformly; and the method is suitable for industrial seedling raising and scale culture.

A conventional Dryopteris erythrosora spore propagation method is mainly characterized in that Dryopteris erythrosora spores are prepared into suspension and the suspension is evenly poured onto mediums contained in an earthen pot by using a syringe; sowing mediums are mixtures of sand, peat and pearlite which are mixed by volume ratio of 1:1:1; the earthen pot into which the spores are sown is put into a transfer case, the height of the water-soaked bottom of the transfer case is 1.5cm and the water-soaked bottom of the transfer case is covered by plate glass; the spores are cultured in the sunshade position of a greenhouse at 25 DEG C to 35 DEG C under the condition that direct sunlight is avoided; juvenile sporophytes are obtained after culture and are respectively transplanted into transplanting mediums I which are formed by leaf mould, peat and sand in an evenly mixing way by volume ratio of 1:1:1, transplanting mediums II which are formed by sand, pearlite and peat in an evenly mixing way by volume ratio of 1:1:1, transplanting mediums III which are formed by peat and garden mould in a mixing way by volume ratio of 1:1, and reference mediums which are formed by garden mould; and the juvenile sporophytes are cultured under the same conditions. By using the characteristic that the germination and the fertilization of the spores need water and by applying pot planting, water soaking and moisture preservation to Dryopteris erythrosora spore propagation, the germination rate of the Dryopteris erythrosora spores, the seedling rate and the survival rate of the transplant sporophytes are effectively improved; in the propagation process, any sterilization process is not required, the processes are reduced, the pollution is reduced, the cost is saved, the operation is simple and convenient and the rapid propagation and the mass production of seedlings are facilitated; the survival rate of the transplanted juvenile spore seedlings reaches more than 85 percent.

The invention provides a fermentation method for preparing nuclease P1, which using directly penicillium citrinum sporogon as seed, fermenting by means of fed-batch sugar supplement to improve activity of the nuclease P1, the mehod without first and second order seed culture shorts production cycle greatly and raises efficiency. The invetion also uses obtained enzyme for preparing 5'-mononucleotide with 85-90% rate of enzymatichydrolysis and 15 mg/ml of producing rate of nucleotide. The invention also provides a penicillium citrinum 3.2788-Y01 of nuclease P1, which fungus stroing number is CGMCC NO.1943, the stem can raise one times active unit of the nuclease P1.

The invention discloses a seedling producing technique for rat-tailed algae, applying to seedling breed in-door according to sexual reproduction law, including: selecting and collecting the seed algae, stimulating and diffusing the seed algae, collecting the juvenile sporophyte, culturing and catadromousing seedlings, provided an artificial seedling producing technique for rat-tailed algae, dissolving the current shortcoming problem of the rat-tailed algae seedlings.

The invention discloses a rapid breeding method of Sargassum fusiforme. The method includes the steps of selecting parents, performing isolation cultivation to single plants, promoting maturity, allowing fertilization, screening juvenile sporophytes, cultivating the juvenile sporophytes and the like. During isolation cultivation of the single plants, the weight ratio of the parents to cultivation water is 1:100; maturity promoting liquid is natural seawater added monopotassium phosphate, calcium chloride and sodium ethylene diamine tetracetate, under salinity of 27%. to 28%.; during fertilization, the ratio of females to males is 5:1. The method has the advantages that the technical defect of germplasm degeneration in the prior art is overcome, high-quality seedlings of Sargassum fusiforme can be obtained, plant dry weight of Sargassum fusiforme bred by the method is up to 247.7g, sac diameter is up to 4.1mm, and by the use of the method compared to the traditional methods, yield per mu of the plant dry weight is increased by 26.1%, the sac diameter is increased by 41.1%, and economic benefit is increased by 37.8%.

The invention discloses a breeding method for Alsophila spinulosa, in particular to a breeding method for plants (Alsophila spinulosa(Wall.ex Hook.)Tryon). The breeding method for Alsophila spinulosa which is provided by the invention is that spores of Alsophila spinulosa are vaccinated in an MS culture medium and cultivated after disinfection, and are replanted and managed to finally gain spore seedlings. Using small amount of spore material, the invention can quickly breed large numbers of spore bodies which can become seedlings for about 70 days, and the planting survival rate can reach more than 80%. The invention overcomes the shortcomings of the current Alsophila spinulosa breeding technique of long breeding period, high cost and low efficiency, and starts a new path of breeding Alsophila spinulosa by people quickly and enables industrial manufacture of Alsophila spinulosa on a large scale, thereby satisfying demands of Alsophila spinulosa from the markets and simultaneously reducing damage to wild resources and environment. In addition, the invention provides an excellent experiment system for starting researches in the aspects of the genetic breeding of species and for protecting the biology and the like, and the invention has extensive application prospect.

The invention relates to a spore reproducing method for Dryopteris varia (L.) Ktunze which can be reproduced fast and is characterized in that the disinfected Dryopteris varia (L.) Ktunze spore is cultured in the culture medium of spore germination and grows into laminar prothallium which is then cultured in multiplication medium for 30 days, in sporophyte inductive medium for 40 days and in sporophyte subculture medium for 20 days in sequence; after forming juvenile sporophyte and grows two to three pieces of juvenile sporophyte leaf, the prothallium is cultured in sporophyte rooting medium for 30 days, is transplanted out of bottle after the base part grows brown fine roots with scales and is transplanted to the soil matrix for culturing after seedling is trained. The spore reproducing method of the invention has little investment and simple equipment, only needs 110 days from the spore germination to the forming of the juvenile sporophyte, obtains large quantities of Dryopteris varia (L.) Ktunze seedlings in short time with the survival rate of the juvenile sporophyte of 90 percent, and leads the Dryopteris varia (L.) Ktunze to become an excellent foliage plant and perform the medical value thereof.

The invention discloses a mass pteridophyta reproduction method. The mass pteridophyta reproduction method includes the steps of (1), harvesting pteridophyta spores; (2), sowing, namely, mixing collected spores with a hormone solution, spraying the mixture into a sowing medium, and reproducing by keeping relative air humidity above 85% and temperature ranging from 25 DEG C to 27 DEG C, wherein the hormone solution consists of 1/2 MS (Murashige and Skoog) culture medium, NAA (naphthalene acetic acid) and GA3; (3), performing 'two-time transplant', namely, transplanting gametocytes on the culture medium after the gametophytes mature and before sporophytes arise, and transplanting seedlings into yellow soil when the sporophytes have 2-3 leaves. By the aid of the mass pteridophyta reproduction method, spore germination rate can be increased, and prothallus development can be promoted; production sowing quantity is guided effectively, and sporophyte growth and transplant management are benefitted; gametophyte fertilization is further benefited by 'two-time transplant', and seedling growth uniformity can be improved while seedling survival rate can be increased.

The invention discloses a method for collecting seedlings from scytosiphon filaments. The heterothallic characteristics of scytosiphon gametes are used for acquiring filaments from discoid bodies formed by parthenogenesis (etheogenesis) of the gametes obtained from individual mature algae, the method for collecting seedlings from filaments is used for chopping and spreading the filaments on an attaching base, and filament cells on the attaching base can directly germinate and form monoploid scytosiphon seedlings. The filaments of propagation culture can be preserved for a long time, the seedling collecting time and the seedling collecting quantity can be determined according to production requirements every year, the culture phases of zygote collection, discoid sporophyte growth, sporangium maturity and the like in the prior art are omitted, and the indoor culture time can be saved by more than 5 months. The invention basically overcomes the defects of season limitation of scytosiphon collection, zygote insemination and alga propagation, long indoor culture time, low success rate of culture of seedlings and the like, reduces the cost of culture of seedlings, greatly improves the success rate of culture of seedlings, provides a large number of seedlings for scytosiphon production, and industrializes the scytosiphon planting industry.

A process for breaking the wall of ganoderma sporophyte and extracting includex mixing the ganoderma sporephytes with solvent, ultrasonic pretreating and high-pressure treating under 250-550 MPa for 5-20 min.

A process for culturing the seedlings of Sargassum fusiforme by inducing reproduction and controlling fertilization includes such steps as providing seawater tank, choosing the seed sargassum fusiforme with male and female receptacles, controlling circulating time, illumination intensity and temp for synchronous mature and discharge of sperm and egg, fertilization, collecting fertilized eggs, inoculating the young sporophytes to attachment, and culturing in seawater at 20-25 deg.C for 1-2 months.

The invention discloses a potted ornamental Stenoloma Chusanum Ching spore propagation and maintenance method, which relates to a spore propagation method for ferns. The method is to propagate sporophytes by using spores of Stenoloma Chusanum Ching and maintain the sporophytes. The method comprises: adding 100 to 200 milligrams of spores into 100 to 200 milliliters of cold boiled water, exhausting air for 1 hour, removing floating matters and water fluid, adding 500 to 1,000 milliliters of cold boiled water to prepare spore suspension, spraying each 500 milliliters of suspension onto 1 to 2 square meters of substrate, covering with a thin film after the substrate is completely wetted by the cold boiled water, and culturing under conditions of a temperature of 20 to 28 DEG C, a light intensity of 5,000 to 20,000Lx and a light period of 10 to 12 h/d till light intensity comes out. For maintaining the sporophytes, bamboo leaf humus soil is used as a substrate, the temperature is kept above 10 DEG C, the light intensity is kept between 10,000 to 50,000Lx, the water content in the substrate is 85 to 90 percent, the ventilation is high, and the bamboo leaf humus soil or tea leaf residueis sprayed every 15 to 30 days. The propagated Stenoloma Chusanum Ching sporophytes can be used as an ornamental and medicinal plant resource.

The invention discloses a pteridophyte seedling raising device. The pteridophyte seedling raising device comprises a seedling raising basin, a sowing clapboard, and a culture medium pushout constructional element. The seedling raising basin comprises a basin body (2) and a cover body (3) which is matched with the basin body. The basin body is composed of a bottom wall and side walls. Through-holes are formed in the bottom wall. The cover body is composed of a top wall and side walls. Convection holes are formed in the side wall of the cover body. The sowing clapboard (4) is arranged in the basin body and is composed of clapboard side walls and side walls of griddings arranged inside the clapboard side walls. The culture medium pushout constructional element (5) comprises a base seat and a convex circular cylinder which is arranged on the base seat and matched with the shape of gridding cavities. The pteridophyte seedling raising device can improve efficiency of seedling and transplanting, reduce cross infection probability between sporophytes, reduce damage of gametophytes and sporophytes in transplanting process, and has the advantages that cultivating room is made full use of, stability of development environment of spores and seeding transplanting process standardization are guaranteed, management cost is reduced and the pteridophyte seedling raising device is suitable for pteridophyte seedling standardization and large-scale production.

The present invention relates to a pteridophyte propagation method in limestone district, belonging to the field of flowers, plants and Chinese medicinal herbs cultivation technology. Said method includes the following steps: collecting spores in spore ripening season of pteridophyte, placing the collected spores into a clean container, placing said container in a ventilating shade place and making it be naturally dried; in 2-3 month of spring sowing the spores into the moss cluster grown on the nightside limestone, after the sporophyte is grown for 20-30 days, transplanting the sporophyte together with moss cluster into green-house or flowers nursery garden to make cultivation.

The invention discloses a method for establishing an in-vitro regeneration system of Osmunda vachellii, which belongs to the field of agricultural biotechnology. According to the method, sporangiorus is used as explant; after surface disinfection, the explant is inoculated into a variety of mediums containing an improved Beneck basal medium; prothallus is obtained through in-vitro germination of spores in sporangium; a part of the prothallus can further undergo enrichment culture, while the other part of the prothallus can be used for induced production of juvenile sporophyte (a sapling); and the sapling is transplanted into a matrix after rooting culture, and the seedling of Osmunda vachellii is formed eventually. The method provided by the invention can realize rapid and continuous obtainment of a great number of high-quality seedlings.