45 results about "Sporophyte" patented technology

The invention relates to a direct seeding and seedling raising method for cibotium barometz spores, and belongs to the technical field of plant planting. The invention provides a direct seeding seedling raising method for cibotium barometz spores. The method comprises the following steps: directly seeding the spores to obtain sporophyte bud seedlings; transplanting the sporophyte bud seedlings to obtain sporophyte seedlings; seedling hardening is carried out on the sporophyte seedlings; the sporophyte seedlings are subjected to seedling hardening when the height is 10-15 cm and the number of leaves is 3-4. By means of the direct seeding seedling raising method, the seedling yield is remarkably increased, the seedling yield of the obtained sporophyte seedlings is 85% or above, large-scale industrial popularization and planting can be conducted, and the market supply amount is guaranteed.

The invention discloses a method for cultivating kelp seedlings through diploid female gametophyte parthenogenesis, and belongs to the field of aquatic product engineering genetic breeding. And hybridizing the female and male single cell cloning lines of the induced protonema to obtain the tetraploid sporophyte. And the tetraploid sporophyte is mature and releases zoospores, diploid gametophytes are germinated and formed, and then male and female separation is performed to obtain diploid female gametophyte clone lines in batches. Under the culture condition provided by the invention, parthenogenesis of a diploid female gametophyte clone line is utilized for seedling culture. The diploid female gametophyte begins to ovulate after 6-7 days, and the ovulation time of the diploid female gametophyte is obviously earlier than that of haploid gametophyte in the same period and under the same condition. The 12-day ovulation rate reaches 86.5%, the 24-day sporophyte conversion rate reaches 98.3%, and most sporophyte forms are normal. In the seedling raising period, parthenogenesis seedling raising of the diploid female gametophyte is completed. The diploid female gametophyte can be used as a brand-new kelp germplasm material to be applied to kelp seedling cultivation.

The invention discloses a cibotium barometz sporophyte transformation method and system based on multi-factor coupling regulation, and relates to the technical field of biology. The method comprises the following steps: synchronously acquiring environment data and sporophyte image data; preprocessing the environment data, performing feature extraction on the image data, and splicing the processed data into a comprehensive feature vector; quantifying the weight, correlation degree and interaction level among the environmental factors through an entropy method, grey correlation analysis and a coupling coordination degree model; constructing an improved CNN-LSTM neural network, extracting features through a double-branch structure, fusing a coupling analysis result to carry out cross-modal fusion, and training to obtain a prediction model; when the predicted conversion rate is lower than a threshold value, environment parameters are automatically adjusted until conversion is completed; according to the method, the problems of environmental factor data isolation and regulation lag in the prior art are solved, accurate prediction and intelligent regulation of the sporophyte transformation process are realized, and the transformation success rate and the production efficiency are remarkably improved.

The invention discloses a method for cultivating triploid kelp sporophytes and identifying ploidy of induced protonema, and belongs to the field of kelp breeding. The method is based on a capillary glass needle separation technology for inducing filamentous somatic cells by kelp and a molecular biological technology for specific marking of sex of kelp gametophytes. The sex specific marker of the kelp gametophyte is used for carrying out PCR (Polymerase Chain Reaction) amplification and 1.5% agarose gel electrophoresis detection on the separated induction protonema single-cell cloning line, so that the genetic sex of the kelp induction protonema can be accurately identified; and the ploidy of the induced protonema of a part of varieties (lines) is directly determined from the molecular level. The method for synchronously detecting the genetic sex and ploidy of the induced protonema breaks through the bottlenecks of chromosome counting and flow cytometry operation, can be completed only through one-time PCR amplification, and is simple, convenient, rapid, short in time consumption, capable of completing detection of a plurality of samples at the same time and low in cost.

This invention provides the nucleotide and amino acid sequences of the Dip gene, as well as its (functional) homologs, fragments, and variants, which provide ploid sporophyte formation as part of apomixis. It also provides ploid sporophyte-forming plants, methods for their preparation, methods for using them, and methods for preparing apomixis seeds.

The invention relates to genetic breeding, in particular to a method for cultivating kelp triploid by inducing a monosexual dihaploid (DH) gametophyte. The method specifically comprises the following steps: establishing a kelp DH sporophyte culture system, inducing DH sporophytes to generate unisexual DH gametophytes (2n) through spore-free propagation, and then hybridizing the unisexual DH gametophytes (2n) with haploid (n) gametophytes with opposite sex to obtain kelp triploid (3n) sporophytes. And determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometry, thereby identifying the chromosome ploidy of the hybrid sporophyte as 3n. The method can be used for cultivating new varieties of high-yield, high-quality, stress-resistant and sterile kelp, and has important significance for developing genetic breeding and new variety creation research in kelp and even kelp brown algae.

The invention discloses a sargassum fusiforme fertilized egg suspension culture new germplasm creation and evaluation method. The method comprises the steps of wild sargassum fusiforme geographical parent selection, isolated culture and unit quality evaluation. Screening male and female plants of parent high-value character strains, performing isolated culture, and preventing and treating harmful organisms; removing epiphytic harmful organisms of male and female plants of the high-value character strain; creating an optimal environmental factor for indoor temporary rearing of lateral branches of male and female plants of the high-value character strain; indoor temporary rearing treatment and temporary rearing time of lateral branches of male and female plants of the parent high-value character strain; classifying and collecting new germplasm fertilized eggs, removing impurities, cleaning, and creating a suspension system of the new germplasm fertilized eggs; preventing and controlling typical harmful organisms of a suspension system in a suspension growth and development stage from new germplasm embryos to juvenile sporophytes; carrying out rope clamping and indoor temporary rearing on new germplasm juvenile sporophytes; stocking the new germplasm juvenile sporophytes in a sea area; an evaluation method of new germplasms of parents and filial generations; a scientific and standard operation and evaluation method can be provided for new sargassum fusiforme germplasm creation, new germplasm quality evaluation and new variety cultivation.

The present invention relates to the field of plant tissue culture technology, and in particular to a method for cultivating Alsophila spinulosa. The method comprises the following steps: inoculating a disinfected spore suspension into a culture medium for cultivation; transferring germinated spore prothallus to an induction GGB culture medium for cultivation; cutting the GGB into small pieces and transferring them to a proliferation culture medium for cultivation; inducing sporophytes and rooting using an incomplete tissue culture method to obtain rooted seedlings; transferring the rooted seedlings to an outdoor greenhouse for cultivation to obtain tissue culture seedlings, and transplanting the tissue culture seedlings onto a mixed substrate to obtain Alsophila spinulosa seedlings. The method provided by the present invention has the characteristics of a high spore germination induction rate, a large GGB proliferation multiple, a high sporophyte induction rate, a high rooting rate, a well-developed root system, sturdy rooted seedlings, a high transplant survival rate, robust seedling growth, and low seedling cultivation costs, thereby providing an efficient and stable tissue culture and rapid propagation technology system for Alsophila spinulosa using the GGB pathway.

The application discloses a method for regenerating direct organs of Cyatheasporophyte leaves, and relates to the field of in-vitro rapid propagation technology.The method induces spores from Cyatheasporophyte protoleaves, then induces green globular bodies (GGBs) from the spores, and after the GGBs germinate into new spores, the new spores are used to induce spore leaf clusters through a leaf direct organ pathway, and the regenerated spore leaf clusters are subcultured and rooted to form complete plants.The method provided in the application has a high spore propagation coefficient and a short cycle through the spore leaf direct organ pathway.

This invention relates to a method for the germination of Cibotium barometz spores and a method for the propagation of Cibotium barometz, belonging to the field of plant culture technology. The propagation method of this invention includes the following steps: disinfection: disinfecting Cibotium barometz spores with a 10% sodium hypochlorite solution for 5-10 minutes; spore germination: the germination medium used includes 1 / 4 MS medium, 4.5-7 g·L⁻¹. ‑1 Agar and 0-30 g·L ‑1 Sucrose; Sporophyte differentiation: The sporophyte differentiation media used included 1 / 2 MS medium and 0-1 mg / L... ‑1 6-BA, 0.5-1 mg·L ‑1 2,4-D, 4.5-7 g·L ‑1 Agar and 25-30 g·L ‑1 Sucrose; Sporophyte strengthening: The sporophyte strengthening medium used included 1 / 4 MS medium, 4.5–7 g·L⁻¹. ‑1 Agar, 30 g·L ‑1 Sucrose and 1-2 g·L ‑1 Activated carbon; hardening and transplanting: After the tissue culture seedlings are sealed, the seal is gradually lifted, and they are then transferred to a specific mixed substrate for cultivation to obtain commercial seedlings. This method can improve the spore germination rate by using a specific sterilization procedure, and achieves the effect of producing more and better seedlings by using specific culture media and hardening preparations at each stage.

The application discloses a kind of Sargassum fusiforme fertilized egg suspension culture new germplasm creation and evaluation method, including wild Sargassum fusiforme geographical parent selection, isolation breeding and unit mass evaluation;Parent high-value character strain female and male plant screening, isolation breeding and enemy biological control;High-value character strain female and male plant epiphytic enemy biological removal;High-value character strain female and male plant lateral branch indoor temporary nursing optimum environmental factor creation;Parent high-value character strain female and male plant lateral branch indoor temporary nursing processing and temporary nursing time;New germplasm fertilized egg classification collection, impurity removal and cleaning, new germplasm fertilized egg suspension system creation;New germplasm embryo to young sporophyte suspension growth and development stage suspension system typical enemy biological control;New germplasm young sporophyte is clamped and indoor temporary nursing;New germplasm young sporophyte is put in sea area;Parent and offspring new germplasm evaluation method;Can be Sargassum fusiforme new germplasm creation, new germplasm quality evaluation and new variety cultivation provide scientific, standard operation and evaluation method.

The invention provides a spore propagation method of cibotium barometz, and relates to the technical field of plant propagation. The method provided by the invention comprises the following steps: sowing Cibotium barometz spores in a composite culture medium, culturing under the conditions of natural illumination and normal temperature, promoting gametophyte transformation by adopting atomized water spraying instead of traditional oscillation insemination, and culturing seedlings in combination with a non-woven fabric seedling culture container. By means of environmental conditions such as natural light and the like, the bionic technology is adopted for breeding, daily management and protection are simple and economical, low-cost, rough-management and efficient batch seedling raising are achieved, the rate of converting gametophytes into sporophytes is greatly increased, the period from spores to transplantable seedlings of cibotium barometz is shortened to 70-80 days, the transplanting survival rate can be increased to 100%, and the survival rate of cibotium barometz is increased to 50%. And a seedling raising guarantee is provided for batch planting of cibotium barometz.

This invention belongs to the field of plant molecular breeding, and specifically discloses a gene related to rice fertility restoration. OsRf19 This invention addresses the cumbersome process of identifying restorer lines for the existing Fujian abortive cytoplasmic male sterility (CMS-FA / OsRf19) system by developing a method for creating tightly linked artificial haplotypes, the development of InDel molecular markers, and their application in rice fertility. Through bioinformatics analysis, this invention identifies a fertility restoration gene associated with the rice sporophytic sterility system CMS-FA. OsRf19 A specific region closely linked to the PPR gene cluster and with extremely low similarity to the loci was edited using CRISPR / Cas12a gene editing technology, resulting in multiple large deletions. Based on these genotype sequence variations, a set of InDel molecular markers was developed to realize the editing of this artificially created restoration gene. OsRf19 Rapid identification of closely linked artificial haplotypes accelerates the breeding process of molecular marker-assisted selection in the rice CMS-FA system.

Methane production in ruminants can be reduced by improving rumen fermentation efficiency through dietary supplementation with Asparagopsis taxiformis. This disclosure relates to a method for producing A. taxiformis and its use. In a specific form, this disclosure relates to a method for producing tetrasporophytes and gametophytes of A. taxiformis by environmental manipulation.

The invention relates to genetic breeding, in particular to a method for cultivating undaria pinnatifida triploid sporophytes by inducing homozygous diploid gametophytes. The method comprises the following steps: inducing homozygous 2n undaria pinnatifida sporophores to produce homozygous 2n gametophytes through spore-free propagation, and hybridizing the homozygous 2n gametophytes with common haploid (n) gametophytes to obtain undaria pinnatifida triploid (3n) sporophores; and determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometer, thereby identifying the ploidy of the chromosome as 3n. The 3n sporophytes are transplanted to the sea for cultivation, and the 3n sporophytes are identified to be incapable of releasing zoospores in the undaria pinnatifida seedling raising (breeding) season, namely infertile. The method can be used for cultivating a new variety of high-yield and high-quality undaria pinnatifida, and has important significance in developing genetic breeding and new variety creation research in undaria pinnatifida and even kelp brown algae.

The invention discloses a tissue culture method for botrychium ternatum spore induced prothallium. The culture method comprises the following steps: collecting botrychium ternatum with sporangium, drying, sieving and collecting spores, sequentially carrying out multiple times of disinfection and cleaning pretreatment on the collected spores by using 75% ethanol, sterile water, 2% sodium hypochlorite and the like in a sterile environment, uniformly coating a culture medium with treated spore suspension, and carrying out observation culture, the time from spore inoculation to prothallium formation is 24-26 days, and the time from spore inoculation to juvenile sporophyte formation is 47-63 days. The culture medium consists of the following components: a 1 / 8 MS culture medium, 5g / L of sucrose and 8g / L of agar. The culture medium is simple to prepare, can effectively induce spore germination and prothallus proliferation of the botrychium ternatum, can be further cultured to obtain sporophytes, can also be used for acclimatization and transplantation, shortens the seedling raising period, further realizes large-scale cultivation of the botrychium ternatum, and has important significance for expanded application of pteridophyte.

The application provides a live sowing and seedling raising method for Dryopteris filix-mas spores and belongs to the technical field of spore propagation of medicinal ferns. The live sowing and seedling raising method for Dryopteris filix-mas spores comprises the following steps: collecting Dryopteris filix-mas spores, sowing on the surface of a culture medium, spraying nutrient solution according to different growth periods, and growing into spore bodies; the culture medium is composed of humus soil, peat soil and limestone at a volume ratio of 3-5:1-3:1-2; during sowing and germination of prothallus, 1 / 8MS+0.01-0.05mg / L GA3+0.3-0.7mg / L 6-BA nutrient solution is sprayed; during the period from prothallus to spore body growth, 1 / 2MS+0.3-0.7mg / L KT+0.3-0.7mg / L 6-BA+1.5-2.5mg / L IBA nutrient solution is sprayed. The application can significantly improve the emergence speed and propagation efficiency of Dryopteris filix-mas by using a specific culture medium and supplementing suitable nutrient solution, and has important significance for realizing artificial planting and resource protection of live sowing and seedling raising of Dryopteris filix-mas.

The invention discloses a module for preparing male gametophyte sterility and a preparation method and application thereof, the module is formed by connecting a pollen specific promoter, an interference element of an OsPTDa gene and an interference element of an OsPTDb gene in series through tRNA-Gly, and the gametophyte male sterility module can be obtained after the elements are assembled. The gametophyte male sterility module disclosed by the invention can down-regulate the expression of male gamete development related genes OsPTDa and OsPTDb at the same time, so that the pollen insemination ability is lost, gametophyte male sterility is generated, and the gametophyte male sterility module plays an important role in preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

The invention provides a kelp sex specific molecular marker and application thereof, wherein the nucleotide sequence of a male marker fragment is SEQ ID NO: 1, and the nucleotide sequence of a female marker fragment is SEQ ID NO: 2. A gene sequence fragment obtained by screening is from a conserved homologous gene in a sex determining region of brown algae. The screened sex identification marker primer sequence is from a region with the highest similarity in a homologous gene coding sequence, the sex of the kelp gametophyte can be rapidly identified only through a pair of degenerate primers, the efficiency is high, the accuracy is high, and the interspecific universality is high. The method is suitable for identification of male and female mixed samples, distinguishing of parthenogenesis source and sexual reproduction source kelp sporophytes and sex identification of gametophytes of other species of kelp, and important support is provided for efficient management and research and utilization of a kelp gametophyte germplasm bank.

The invention discloses a gametophyte male sterility related gene OsPTDb and application thereof. The amino acid sequence of the OsPTDb gene is as shown in SEQ ID NO. 1; the OsPTDb gene encodes an ascorbic acid oxidase which is an essential gene for male gametophyte development, the insemination of the male gametophyte cannot be completed and gametophyte male sterility is generated due to the abnormal function of the gene, and the OsPTDb gene plays an important role in the aspects of preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

The present invention relates to the field of expression of recombinant glycoproteins in plants. In particular, the present invention relates to a method of producing a genetically modified plant capable of producing glycoproteins harbouring paucimannosidic N-glycans, to a method of producing a glycoprotein of interest harbouring paucimannosidic N-glycans and to genetically modified plants, plant cells, protoplasts, protonemas, gametophytes, sporophytes or spores produced with or used in such methods.

The application provides a method for artificially breeding Doerryoptis concolor. The method for artificially breeding Doerryoptis concolor comprises the following steps: (1) sowing spores of Doerryoptis concolor in a breeding substrate, and culturing until obtaining sporophyte seedlings; and (2) transplanting the sporophyte seedlings into a rejuvenation substrate, and performing division rejuvenation culture. The method has high germination rate, high survival rate, high growth quality, and fast growth speed, and can effectively meet the demand of the market for Doerryoptis concolor.

Described herein are compounds, formulations, and methods for blocking sporozoite invasion and subsequent liver-stage parasite development of a protozoan parasite, such as Plasmodium falciparum.

The invention discloses a chimeric interference pollen lethal module as well as a preparation method and application thereof. The module consists of a pollen specific promoter, a chimeric interference element of an OsPTDa gene and an OsPTDb gene, and a plant endogenous terminator. After the elements are assembled, pollen can be killed. The pollen lethal module disclosed by the invention can accurately down-regulate the expression of male gametophyte development related genes OsPTDa and OsPTDb in the same time and space, so that pollen germination is abnormal, transgenic pollen cannot generate offspring, gametophyte male sterility is generated, and the pollen lethal module has important value in the aspects of preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

Methane production in ruminant animals may be reduced by improving rumen fermentation efficiency through dietary supplementation of Asparagopsis taxiformis. The present disclosure relates to a method of producing A. taxiformis and uses thereof. In particular forms, the present disclosure relates to methods of producing a tetrasporophyte and a gametophyte of A. taxiformis through environmental manipulations.

The present invention relates to the technical field of plant cultivation, and particularly relates to a method for cultivating Cibotium barometz spores. A method for cultivating Cibotium barometz spores comprises the following steps: S1: After the substrate is formulated and pre-moistened with water, it is subjected to high-pressure sterilization treatment; S2: The sterilized substrate is spread into a seedling tray as the spore sowing substrate; S3: The Cibotium barometz spores are made into a suspension and sprayed onto the seedling substrate in S2, and daily management is carried out until the spores germinate; S4: Once the antheridia and archegonia are formed, it is soaked thoroughly with water to facilitate the combination of sperm and eggs to form a sporophyte; S5: According to the growth situation of the seedlings, potting is carried out until the seedling height is about 6 cm - 10 cm, and when tubers are formed at the roots, the seedling cultivation is completed and transferred to under-forest cultivation; this method has the advantages of fast spore germination, neat emergence, strong seedling condition and high emergence rate.

The invention relates to germplasm preservation, propagation and seedling cultivation of undaria pinnatifida, in particular to a method for large-scale updating and rapid propagation of undaria pinnatifida gametophyte germplasm. Specifically, obtained mature homozygous double (DH) sporophytes are dried in shade and stimulated to diffuse a large number of zoospores, and the spores germinate to form gametophytes consistent with parent genotypes, so that large-scale updating and rapid propagation of gametophyte germplasm are realized. The method disclosed by the invention effectively solves the technical bottleneck that a large number of gametophyte clones are difficult to obtain in a short time due to slow cell division of gametophytes from single cells, and has important significance on creation of new undaria pinnatifida hybrid varieties and large-scale seed production.

The invention discloses a sporophyte fertile gene OsSTRL3 and application thereof. A protein encoded by the sporophyte fertile gene is an amino acid sequence as shown in SEQ ID NO.1, or a protein which is subjected to substitution, deletion or addition of at least one amino acid and has the same function as the OsSTRL3; the nucleotide sequence of an encoding region of the OsSTRL3 gene is shown as SEQ ID NO.2. The invention also discloses a mutation site of the OsSTRL3. The OsSTRL3 gene disclosed by the invention encodes a protease with an isosessile synthetase / phospholactonase structural domain, and has a function of regulating and controlling normal development of pollen, sporophytic male sterility can be generated by mutation of the gene, and the mutation of the OsSTRL3 gene can be used for regulating and controlling normal development of pollen. The strain can be used as a female parent for producing hybrids in heterosis utilization.

The invention discloses a method for rapidly propagating solieria seedlings, and relates to the technical field of solieria cultivation. The invention provides a method suitable for rapid propagation of solieria seedlings in a land-based artificial regulation environment. The whole set of method combines a first-stage propagation process and a second-stage propagation process. When primary propagation is carried out, immature quartophyte or male and female gametophyte fronds are used as propagation mother branches, a plurality of independent offspring seeds are obtained after cleaning, cutting and seedling separation treatment, then the cut independent offspring seeds are inoculated into a culture container of an algae culture chamber for suspension aeration culture, and the offspring seeds are promoted to recover, germinate and grow; during second-stage propagation, the solieria seedlings subjected to first-stage propagation are inoculated into a floating frame with holes and placed in a circulating water seedling propagation pool for rapid propagation. The whole propagation process is completed in a land-based artificial regulation environment, and the method has the advantages of being high in propagation efficiency, short in cultivation period, long in suitable cultivation period, high in seedling survival rate and budding rate and the like.