19 results about "Bryophyte" patented technology

The invention discloses a moss-microorganism synergistic nitrogen fixation bionic regreening method and system for a drought-resistant mine rock slope, and belongs to the technical field of ecological environment restoration. The method comprises the following steps: (1) slope pretreatment: drilling anchoring holes; (2) layered construction: (1) a fixation layer: spraying mixed slurry prepared from amylase and plant fibers to form a net-shaped substrate with the thickness of 1-2cm; (2) a nutrient layer: spraying a mixture of a complex microbial inoculant and a slow release fertilizer, wherein the thickness is 4cm; the mixed bacterial agent is prepared from the following raw materials in percentage by weight: 60 percent of phytonimum patenum spores, 25 percent of nostoc commune (Nostoc commune) and 15 percent of pseudomonas fluorescens (Pseudomonas fluorescens), and the mixed bacterial agent is prepared from the following raw materials in percentage by weight: 60 percent of phytonimum patenum spores, 25 percent of nostoc commune (Nostoc commune) and 15 percent of pseudomonas fluorescens (Pseudomonas fluorescens). And (3) a water retention layer: sodium polyacrylate resin is spread, the particle size of the sodium polyacrylate resin is 1-0.5 mm, soil covering and compaction are performed until the thickness of the water retention layer is 1-1.5 cm, and the use amount of the sodium polyacrylate resin is 2-1.5 wt% of the weight of the soil. According to the method, the nitrogen fixation efficiency can be improved by 40-60%, and the soil moisture content is kept gt under the drought condition; the survival rate of the seedlings is increased to 85% or above, the anti-scouring capacity of the side slope is improved by 50%, and the vegetation coverage rate of 70% or above is achieved within 6 months.

The invention provides a metal mine ecological restoration method based on trapezoid layered stacking and moss inoculation, and belongs to the technical field of metal mine reclamation ecological restoration and ecological environment improvement. Slope planting point locations are determined; selecting a moss plant community planting scheme according to a greenhouse; and according to preset time, bryophytes, shrub plants, arbor plants and herbaceous plants in the ecological restoration area are subjected to complementary planting and tending, so that the metal mine waste rock heap forms an ecological plant community, and the preset time is preferably two growing seasons. According to the method, a single greening mode in the greening process of the metal mine is broken through, a mode for carrying out vegetation restoration on the mine by adopting the moss is provided, a new way is opened up in the aspects of metal mine soil fixation and ecological restoration, the means and technology of ecological restoration of the metal mine are enriched, and the method has good popularization and application prospects.

A self-watering system for irrigating plants comprising a planter divided by a partition wall separating the planter into two parts a growing medium container and lower part a liquid reservoir, the partition wall having an aperture for a liquid movement device and plurality of aeration apertures, the growing medium container having plurality of air inlets at the base. The system comprising a liquid movement device for moving liquid via hydrostatic pressure and capillary action. The liquid movement device comprises a hollow body, rock wool or bryophyte inside the hollow body, and a liquid permeable material inside a bag of interwoven materials. The liquid permeable materials cover an opening of the hollow body. A layer of rock wool or dry compressed bryophyte covers the bag.

The invention discloses a method for preparing a botanical insecticide from a liverwort extracting solution and application, and relates to the field of botanical insecticides, the method comprises the following steps: collecting and pretreating a liverwort plant material; the method comprises the following steps: repeatedly extracting a liverwort plant material for multiple times by adopting a lipophilic organic solvent, and collecting an extracting solution; carrying out multi-stage solid-liquid separation and purification on the extracting solution, removing impurities and collecting clarified supernate; performing vacuum concentration and directional purification on the supernate to obtain a high-concentration liverwort extract concentrated solution; mixing and homogenizing the concentrated solution of the liverwort extract and auxiliary materials to prepare a botanical insecticide preparation; according to the method for preparing the botanical insecticide by utilizing the liverwort extracting solution and the application, a moss plant resource, namely liverwort, is applied to the botanical insecticide for the first time, and insecticidal active ingredients of the liverwort are enriched by adopting a specific extraction process, so that synchronous and efficient prevention and control of two types of pests with different mouthparts can be effectively realized, and the prevention and control efficiency of the botanical insecticide is improved.

This invention provides a DNA barcode suitable for bryophytes, wherein the gene of the DNA barcode is matK, and the primers for matK are matK-D-F and matK-D-R. The sequence of primer matK-D-F is (5'-3')TCGTCATCBGTTAAAGTAGTCC; the sequence of primer matK-D-R is (5'-3')CGTCRACGNATACAAGATGCT. When using the new matK primers for PCR amplification and sequencing of test materials, the success rate of both PCR amplification and sequencing reaches 100%, and different species can be distinguished. The new matK primers matK-D-F and matK-D-R have good versatility and high resolution for bryophytes.

This invention discloses the construction and application of a biocrust based on algae-fungus-bryophyte symbiosis on bare red soil slopes, belonging to the field of ecological restoration technology. By screening dominant species of algae, microorganisms, and mosses suitable for acidic, nutrient-poor, and highly volatile red soil habitats, and optimizing the symbiotic ratio and inoculation / planting techniques, a stable and highly efficient algae-fungus-bryophyte symbiotic biocrust is constructed. This biocrust can significantly improve the physical and chemical properties of bare red soil slopes, enhance soil water retention and interception capacity, inhibit ineffective water evaporation, promote seed germination and seedling establishment, and enhance slope erosion resistance. The construction method of this invention is simple to operate, low in cost, ecologically adaptable, and fast in planting, enabling rapid vegetation cover and ecosystem restoration of bare red soil slopes. It is applicable to ecological management and restoration projects in various bare red soil slope areas, such as abandoned mining sites, highway / railway slopes, bare red soil hillsides, and soil erosion areas.

The present application relates to the field of fly ash heavy metal stabilization, and relates to a method for stabilizing fly ash heavy metal using natural extract, comprising the following steps: mixing biomass tar generated by pyrolysis of seed plants with an organic solvent to obtain a mixed solution; drying seed plants, bryophytes and algae plants to constant weight, then mixing them with each other and with the obtained mixed solution to carry out a mixing reaction, and then filtering a liquid phase; taking the obtained liquid phase, extracting and concentrating, and then immersing fly ash in the concentrated solution and stirring to carry out heavy metal stabilization. Compared with the prior art, the present application has the advantages of good metal stabilization effect, green environmental protection and the like.

The present invention relates to a glyphosate-sensitive bryophyte with reduced expression of the gene encoding UDP-N-acetylglucosamine enolpyruvyl transferase (MurA); and / or reduced MurA activity relative to a corresponding wild-type glyphosate tolerant bryophyte. The invention also related to methods of generating said glyphosate-sensitive bryophyte, and methods of using said glyphosate-sensitive bryophyte in screening candidate compounds for EPSPS inhibitor activity. The invention also relates to the use of the MurA gene in providing resistance against EPSPS inhibitors, and a transgenic plant comprising a MurA transgene.

This utility model relates to the field of drip irrigation technology for artificially cultivated bryophytes, and more particularly to a shallow-buried drip irrigation device for artificially cultivated bryophyte crust formation. It includes an installation platform; a water pump is mounted on the upper part of the platform, with a water pipe connected to the input end of the pump. A connector is connected to the end of the water pipe furthest from the pump, for connecting to an external water source pipeline. The water pump provides a power source, and a main pipeline is connected to the output end of the pump. The main pipeline receives water from the water pipe, and a drip irrigation assembly is connected to the lower end of the main pipeline. The drip irrigation assembly includes a flow direction switching module, a diversion module, a drip irrigation module, and a water retention module. The flow direction switching module controls the water flow direction, and the diversion module distributes the water from the main pipeline to each drip irrigation module. This utility model uses an absorbent rope to transport water from a lower position upwards to an absorbent felt, which absorbs and stores water, creating a continuously moist microenvironment at the roots of the bryophyte and improving water utilization.

The invention belongs to the technical field of bryophyte cultivation, and particularly discloses a cultivation method of hypnum melioides, which comprises the following steps of: constructing three layers of substrates (the bottom layer is planted with golden stones of 3-5mm, the middle layer is peat soil-Kanuma soil of 1: 0.8-1.2, and the upper layer is plasma treated felt cloth); the broken stems are pretreated with an activating solution containing naphthylacetic acid and chitosan; after inoculation, culturing in a full-artificial light environment (800-1200lux, the ratio of blue light to red light is 1: (2-3)), and intermittently spraying to maintain the humidity to be 85-90%; a nutrient solution containing nitrogen, phosphorus, potassium and trace elements is sprayed every week; and the COO is enriched to 800-1000 ppm in the seedling stage. According to the method, within 21 days, the coverage degree is larger than 80%, the mildew rate is smaller than 3%, the biomass increment reaches 48.7 mg / cm, the water utilization efficiency is improved by 223%, and the method is suitable for large-scale production of vertical greening walls and ecological restoration projects.

This invention discloses a method for enhancing the drought resistance of bryophytes, relating to the field of environmental engineering technology. The method provided by this invention enables artificially cultivated bryophyte crusting materials, whose drought resistance needs to be enhanced, to passively receive induced VOCs to form a stress imprint, self-adjusting their physiological state to enhance drought resistance. This process consumes low material and energy costs, resulting in better plant performance under subsequent stress. It avoids the drawbacks of active methods, such as slow growth and high mortality rates after inoculation, thus exhibiting a greater survival advantage, higher propagation success rate, and greater stability. This method is effective not only during drought but also during rehydration. Furthermore, the method provided by this invention is simple and easy to implement.

This invention discloses the application of the *Rhizopus dentata* ScPRT1 gene in regulating plant responses to abscisic acid (ABA), the nucleotide sequence of which is shown in SEQ ID NO:1. This invention found that *Arabidopsis thaliana* lines overexpressing the *Rhizopus dentata* ScPRT1 gene, or *Rhizopus dentata* lines transiently expressing the *Rhizopus dentata* ScPRT1 gene, exhibited significantly reduced ABA sensitivity and significantly enhanced ABA response. Therefore, the *Rhizopus dentata* ScPRT1 gene negatively regulates plant responses to ABA signals. This invention provides resources for the discovery of stress-resistance genes, improves the regulatory network of *Rhizopus dentata* in response to ABA signals, and offers a new perspective for understanding the molecular mechanisms of stress adaptation in desert bryophytes.

The utility model relates to the technical field of bryophyte specimen preparation, and discloses a bryophyte shaping ecological specimen preparation device which comprises a box body, the box body is of a cavity structure with an opening in the top end, a supporting frame is fixedly connected to the inner wall of the top end of the box body, and a top plate making contact with the opening is arranged at the top end of the supporting frame. Symmetrically arranged supporting frames are fixedly connected to the bottom end of the box body, a box opening is formed in one side of the box body, a through hole is formed in the middle of the top plate, a flow guide unit extending into the box body is fixedly connected to the through hole, first ventilation pipes are installed on the top plate at equal intervals, and a ventilation mechanism and an air exhaust unit connected with the flow guide unit are installed on the top plate; ventilation pipes II are equidistantly arranged at the bottom end of the box body; constant-temperature heater bodies are symmetrically mounted on the inner wall of the bottom end of the box body; according to the utility model, the prepared bryophyte specimen is not curled or brittle, and can be kept consistent with the collected bryophyte specimen in shape, so that the shape maintaining function of the bryophyte is effectively realized.

The present disclosure relates to a bryophyte cell that is transformed or genetically modified to express aureusidin synthase and chalcone-4'-glycosyltransferase. The aureusidin synthase and chalcone-4'-glycosyltransferase may be expressed as a single biscistronic cassette separated by a P2A peptide or separately through insertion of two unique gene cassettes into said bryophyte cell. The expression may be driven by an exogenous promoter. The transformed bryophyte cell may be exposed to UV radiation to enhance production of exogenous flavonoids.

The invention provides application of silicon dioxide nanoparticles in promoting moss leaf incision stem cell development and a method, and relates to the technical field of plant regeneration. Depending on the totipotency of the plant stem cells, the silicon dioxide nanoparticles are used for promoting the development of the stem cells of the in-vitro moss leaves subjected to mechanical damage, promoting the rapid growth of leaf incisions, generating more new growth points, remarkably increasing the number of the epiphytic points of the newly born protonema of the leaves, shortening the cultivation time and improving the cultivation efficiency; and an effective nanometer strategy is provided for rapid propagation of moss.

The invention relates to a moss-based biochar material and a preparation method thereof.According to the preparation method, waste moss is used as a raw material, the problem that a carbon material is prepared from traditional waste biomass is solved, and a thought is provided for high-value utilization of bryophytes; the preparation method comprises the steps of moss pretreatment, ammoniation treatment, carbonization activation and formed carbon cleaning, an ammoniation agent is ammonia water, and the ammonia water has excellent pore-forming capacity and is beneficial to improving the adsorption capacity of heavy metal ions; raw materials are easy to obtain, operation steps are simple, production cost is low, popularization is convenient, and large-scale benefit production can be achieved; the invention further relates to application of the moss-based biochar material in preparation of a heavy metal adsorbent, the moss-based biochar material is used for adsorbing heavy metal Ni (II) in wastewater, and experimental verification shows that compared with moss which is not subjected to ammoniation treatment, the adsorption capacity of the biochar material prepared from the moss which is subjected to ammoniation treatment on the heavy metal Ni (II) is 1.15-2.38 times, and the adsorption capacity of the biochar material prepared from the moss which is subjected to ammoniation treatment on the heavy metal Ni (II) is 1.15-2.38 times. A brand-new thought is provided for preparing the heavy metal adsorbent from the waste biomass.

A planter 1 divided by a partition wall 2 separating the planter into two parts a growing medium container 3 and lower part a liquid reservoir 10,the partition wall having an aperture 5 for a liquid movement device and plurality of aeration apertures 7,the growing medium container having plurality of air inlets 8 at the base. A liquid movement device for moving liquid via hydrostatic pressure and capillary action. The liquid movement device comprises a hollow body 12, rock wool or bryophyte 15 inside the hollow body 12, and a liquid permeable material 18 inside a bag 16 of interwoven materials. The liquid permeable materials cover an opening 14 of the hollow body. A layer of rock wool or dry compressed bryophyte 19 covers the bag 16.

The utility model discloses a kind of plant moss ball's assembly device, comprising: base, bottom support mechanism and plant stem support mechanism.Bottom support mechanism is fixedly assembled above base, and bottom support mechanism includes upper limit ring, support tray and several groups of middle limit ring, and upper limit ring, support tray and several groups of middle limit ring cooperate to form spherical support inner groove, and upper limit ring, support tray and several groups of middle limit ring cooperate to form multiple transverse assembly gaps.Plant stem support mechanism is fixedly assembled above upper limit ring, and plant stem support mechanism includes support ring, wear pad and a pair of fixed side pole.The plant moss ball's assembly device disclosed in the utility model can support and limit the plant root system wrapped by soil and the moss to be coated, which facilitates the subsequent coating of the plant root system wrapped by soil with moss, simplifies the subsequent fixing process, and improves the production efficiency of the plant moss ball.

The invention discloses an algae-moss-grass symbiotic crust soil ecological restoration method, and belongs to the technical field of ecological restoration. Comprising the following steps that algae, bryophytes and gramineous herbaceous plants are jointly inoculated into the soil, the illumination condition with the illumination intensity being 3000 Lux to 8000 Lux is set on the soil to be remediated, and water is applied according to the frequency of 1-3 days. The regulation and control scheme can be flexibly adjusted according to different regional climate conditions, the strong-light high-frequency water application combination is suitable for a wet region, the weak-light condition is suitable for a moisture-limited region, the whole process does not need to depend on chemical reagents, and the method is environmentally friendly and can be widely applied to ecological engineering such as soil remediation and slope treatment in arid and semi-arid regions; wide application prospects are realized.