The invention discloses a toadstool substrate and the cultivation technology of the toadstool substrate. A formula of the toadstool substrate is characterized in that 60% of cottonseed hull, 10% of wheat bran, 10% of corncob, 10% of peony root, 5% of apple branch, 1% of glucose, 2% of gypsum and 2% of light calcium carbonate are contained in per 100 kilograms of drying materials, wherein the corncobs, the apple branches and the peony roots are broken to form particles with the diameters of 0.2-0.5 centimeters, and the particles are used after being stored for one year. Multiple changes are carried out on the basis of the original toadstool cultivation technology, the raw materials of the peony roots with special effects are added in the formula, breakthrough technological means are additionally used in technical processing, and the technological means include the steps of (1) finding a symbiotic relationship with the peony roots and adding a defined amount of the peony root particles in the formula, (2) conducting submersible soaking on toadstool outlet fields in good time in the cultivation process, and (3) using a principle for transfusion for people, and providing 10 DEG C-22 DEG C clear water for toadstool primordia. The toadstool cultivation technology is greatly broken through, and a cultivation living example can be used for providing certifications.