498 results about "Lichen" patented technology

The invention relates to a complex microbial inoculant for preventing and treating citrus huanglongbing as well as a preparation method and application of the complex microbial inoculant. The compound bacterial agent for preventing and treating the citrus huanglongbing is prepared from the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. The compound bacterial agent for preventing and treating the citrus huanglongbing comprises the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. After the complex microbial inoculant is added, the content of callose in infected leaves can be effectively reduced, the self-resistance mechanism of plants is induced, the disease resistance is improved, and the complex microbial inoculant has an obvious inhibiting effect and a growth promoting effect on Candidatus Liberibacter asiaticum.

The invention discloses a decomposition preparation containing bacillus licheniformis as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The decomposition preparation provided by the invention comprises bacillus licheniformis and Thermus arcuatus. The bacillus licheniformis is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 27, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from coastal saline-alkali soil, has high temperature resistance and salt resistance, and can secrete protease and lipase at the same time. After the bacillus licheniformis and the Thermus arcuatus are mixed, the synergistic effect is remarkable, the duck-derived red mud is effectively promoted to be converted into humic acid, and high-value resource utilization of the duck-derived red mud is achieved.

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention belongs to the technical field of soil improvement, and relates to a soil conditioner for saline-alkali soil and a soil improvement method. The soil conditioner comprises vinegar residues, chitin, glucose, a composite biological preparation, a modified gel and a functional additive, the composite biological agent comprises bacillus subtilis powder, bacillus licheniformis powder, trichoderma spore powder, brown algae powder and light calcium carbonate; the functional auxiliary agent comprises modified bentonite, polyglutamic acid, pregelatinized starch and biochar potassium humate. Through the synergistic effect of multiple components and structural design optimization, systematic remediation of physical properties, chemical properties and biological activity of the saline-alkali soil is achieved. According to the technology, the limitation of single function of a traditional improvement measure is broken through, and the long-standing technical problems of return salt rebound, low fertility, microbial community imbalance and the like in saline-alkali soil treatment are effectively solved by constructing a multi-layer and multi-dimensional soil improvement system.

The invention relates to the technical field of soil improvement, and discloses a soil improvement repairing agent and a preparation method thereof. The preparation method comprises the following steps: adding biochar, modified eugenol, modified benzothiazolyl pyridine, bacillus licheniformis and diatomite into a mixer, mixing and stirring, extruding and granulating in a granulator, and drying to obtain the soil improvement repairing agent. Eugenol can destroy a membrane structure and denature protein, so that a bacteriostatic effect is achieved, and a healthy soil microenvironment is provided for crops; the quaternary ammonium salt can destroy the membrane structure of pathogenic microorganisms, plays a role in killing the microorganisms, can improve the antibacterial effect of the remediation agent, and prevents and treats spread diseases of soil; nitrogen atoms and sulfur atoms in benzothiazolyl pyridine molecules can coordinate with free heavy metal ions in the soil, the heavy metal adsorption effect of the remediation agent is improved, the production value of the soil is gradually restored, meanwhile, boric acid capable of being absorbed by plants can be released through hydrolysis of pinacol ester, and the fertility of the soil is improved.

The invention discloses a coated slow-release solid water-soluble fertilizer as well as a preparation method and application thereof. The coated slow-release solid water-soluble fertilizer comprises the following components in parts by weight: 15-20 parts of a nitrogen source, 8-12 parts of a phosphorus source, 15-25 parts of a potassium source, 3-8 parts of humic acid and 10-15 parts of charcoal powder, the composite material comprises the following components in parts by weight: 0.5-1.5 parts of functional microorganisms, 1-2 parts of microbial metabolite, 2-5 parts of seaweed extract and 5-8 parts of chitosan-gelatin composite material. The functional microorganisms are bacillus subtilis, bacillus licheniformis and azospirillum which are adsorbed on the charcoal powder. The coated slow-release solid water-soluble fertilizer provided by the invention has the effects of supplying nutrients and preventing and treating soil-borne diseases, achieves multiple effects by one fertilizer, and can improve the crop nutrient utilization rate, inhibit the soil-borne diseases and promote the diversity of soil microorganisms.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention discloses a microbial compound feed for improving disease resistance of blue crabs and a preparation method of the microbial compound feed, and belongs to the technical field of feed preparation. The microbial compound feed comprises the following raw materials in parts by weight: 30-40 parts of fermented soybean meal, 20-25 parts of bran, 15-20 parts of fish meal, 5-8 parts of a compound microbial agent, 3-5 parts of algal polysaccharides, 2-3 parts of a radix astragali extract, 1-2 parts of chitosan, 2-3 parts of a mineral premix and 1-1.5 parts of compound vitamins, wherein the compound microbial agent comprises bacillus licheniformis, lactobacillus acidophilus and saccharomyces cerevisiae. The microbial compound feed disclosed by the invention comprehensively improves the disease resistance of the blue crabs and improves the survival rate through various ways, such as nutrition balance, immune regulation and intestinal health improvement, so that the breeding cost is reduced, and the income of farmers is increased.

The present invention belongs to the technical field of microbial fermentation, and discloses the application of a compound bacterium agent of Lactobacillus rhamnosus and Bacillus licheniformis for reducing acids and higher alcohols, Lactobacillus rhamnosus, and in Baijiu. Lactobacillus rhamnosus L21 has a preservation number of: CGMCC NO. 32609, a preservation date of: November 13, 2024, and a preservation unit of: China General Microbiological Culture Collection Center. The use of the compound bacterium agent of Lactobacillus rhamnosus L21 and Bacillus licheniformis XDNZ_Bl_27 can effectively reduce organic acids such as lactic acid and propionic acid in the Baijiu fermentation system, and at the same time can also reduce the content of n-propanol in the fermented grains, which is beneficial to the regulation of acidity and higher alcohols in the process of Baijiu production.

The invention discloses a composition containing cactus fermentation liquor and a preparation method and application of the composition in cosmetics, the cosmetic composition containing the cactus fermentation liquor comprises the cactus fermentation liquor, Ectoine and sodium hyaluronate according to a specific proportion, the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on succulent stems of cactus, and the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on the succulent stems of the cactus. Sequentially inoculating bacillus licheniformis and lactobacillus plantarum for sequential fermentation; and finally, purifying and enriching by ultrafiltration and nanofiltration two-stage membrane separation technologies. A product rich in small molecule active peptide and specific organic acid is obtained through biotransformation process combination, so that the final cosmetic composition has remarkable skin barrier repairing and anti-oxidation effects.

The invention discloses a planting method for increasing soil organic matters and carbon sequestration, belongs to the technical field of ecological soil improvement, and provides a planting method for increasing soil organic matters and carbon sequestration, which comprises the following steps: applying a complex microbial inoculant on the surface of a planting land; planting an arbor, and then planting caragana twisting in planting gaps of the arbor; the composite microbial inoculant is prepared from bacillus cereus (bacillus cereus) and bacillus parlicheniformis (bacillus parlicheniformis), and the composite microbial inoculant is prepared from bacillus cereus (bacillus cereus) and bacillus parlicheniformis (bacillus parlicheniformis); the arbor is picea crassifolia, pinus sylvestris and pinus tabulaeformis. According to the method, picea crassifolia, pinus sylvestris and pinus tabulaeformis of different forest ages are planted in a mixed mode, and caragana twistable is matched, so that the effects of maximizing soil organic matter accumulation and enhancing carbon sequestration in the Gansu Xieu region are achieved.

The invention discloses a probiotic animal feed and a preparation method thereof, and belongs to the technical field of animal feeds, the probiotic animal feed comprises the following components by mass: 40-55 parts of caragana microphylla powder, 20-30 parts of fermented soybean meal, 3-5 parts of a composite probiotic preparation, 5-10 parts of a medicinal and edible additive, and 2-3 parts of a mineral premix. According to the composite probiotic preparation, bacillus licheniformis, pediococcus pentosaceus and saccharomyces cerevisiae boulardii synergistically improve the fiber decomposition efficiency, inhibit pathogenic bacteria and reduce ammonia nitrogen emission. The medicinal and edible additive is of a multi-layer micro-capsule structure, the outer layer is carboxymethyl chitosan and copper gluconate modified nano zeolite, active substances in the middle layer are rapidly released through rumen pH response, and the middle layer contains a yucca extract, a liquorice extract and other components and can inhibit urease activity and ammonia-producing bacteria; in the inner layer, a nano-selenium-vitamin E compound, curcumin and the like are slowly released through sodium alginate-pectin gel, so that intestinal metabolism is optimized. The process of pretreating caragana microphylla through steam explosion and performing solid-state fermentation on soybean meal through aspergillus oryzae is utilized, so that grain-free production is realized.

The invention provides a 2-succinyl-5-enol acetonyl-6-hydroxy-3-cyclohexene-1-carboxylic acid (SEPHCHC) synthase mutant, which is characterized in that on the basis of a bacillus subtilis SEPHCHC synthase gene MenD, 12 SEPHCHC synthase mutants are obtained by virtue of a site-specific mutagenesis PCR (Polymerase Chain Reaction) technology, and the SEPHCHC synthase gene MenD is used as a base. Recombinant bacteria of bacillus subtilis or bacillus natto or bacillus licheniformis or bacillus amyloliquefaciens for expressing the mutant are respectively constructed, and the yield of heptaene menadione fermented by the recombinant bacteria is 74%-203% of the yield of heptaene menadione fermented by the recombinant bacteria of bacillus subtilis for expressing wild type SEPHCHC synthase MenD.

The invention relates to the technical field of fertilizers, in particular to a fulvic acid type compound microbial organic fertilizer and a preparation method thereof. The method comprises the following steps: preparing compound microorganism bacterium powder from bacillus subtilis, bacillus megatherium, bacillus licheniformis, pseudomonas fluorescens and bacillus psychrosaccharolyticus; preparing a solid-phase substance from ferric chloride hexahydrate, terephthalic acid and dimethylformamide; generating hydrogel on the surface of the chemical fertilizer in situ, and adding a solid-phase substance to obtain a slow-release chemical fertilizer; and preparing an organic fertilizer, and mixing the organic fertilizer with the compound microbial powder, the slow-release chemical fertilizer and the organic fertilizer to obtain the compound microbial organic fertilizer. According to the compound microbial organic fertilizer provided by the invention, the chemical fertilizer, the organic fertilizer and the microbial fertilizer synergistically promote the growth of plants, and the use of the chemical fertilizer is reduced.

The invention belongs to the technical field of agricultural microbial control, and particularly relates to a growth-promoting and disease-preventing compound microbial agent and a preparation method and application thereof, and the compound microbial agent specifically comprises microbial powder, a growth auxiliary material and a drying agent; the microorganisms comprise vermiform yellow, trichoderma harzianum, bacillus velezensis, bacillus licheniformis and brevibacillus laterosporus. According to the compound microbial agent, through the synergistic effect of multiple strains, the control effect of the compound microbial agent on verticillium dahliae is enhanced, and the defects that a single biocontrol bacterium is single in biocontrol mechanism, poor in environment adaptability, incapable of being colonized in soil for a long time and unstable in control effect are overcome; the compound microbial agent can increase the yield of cotton and pepper and improve the quality of cotton fibers, and provides scientific and technological support and decision service for realizing green, environment-friendly, cost-saving and efficiency-increasing modern agriculture.

The invention discloses a method for improving uranium immobilization by bacillus by using an extracellular polymeric substance, and belongs to the field of environmental engineering, the bacillus licheniformis X-113 is separated from a uranium-polluted environment and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 20251207, and the bacillus licheniformis X-113 is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 20251207. By optimizing the EPS culture conditions, the fixing capacity of the EPS-Bacillus sp.X-113 on uranium is remarkably enhanced compared with that of Without EPS-Bacillus sp.X-113, and the fixing capacity of the EPS-Bacillus sp.X-113 on uranium is remarkably enhanced compared with that of Without EPS-Bacillus sp.X-113. And numerous functional groups in EPS, such as carboxyl, hydroxyl, amino and the like, can be subjected to complexation, ion exchange and other reactions with uranium ions to fix the uranium ions, so that the uranium fixation of the bacillus is greatly improved.

The invention provides compost and a preparation method thereof, and relates to the technical field of organic fertilizers, the compost comprises the following components by weight: 900-1100 parts of crop straw, 200-300 parts of animal excreta, 100-200 parts of an organic auxiliary material, and 1.5-7.5 parts of a complex microbial inoculant; the complex microbial inoculants comprise lactic acid bacteria, bacillus licheniformis, thermophilic trichoderma, actinomycetes and white rot fungi. The preparation method of the compost mainly comprises the following steps: stacking the crop straws and the organic auxiliary materials layer by layer, and compacting; uniformly sprinkling animal excrement on each layer, and then inoculating a complex microbial inoculant; punching a hole in the top of the pile to monitor the moisture content in the pile in the composting process; pile turning treatment is carried out in the composting process. According to the invention, the complex microbial inoculants are adopted for compost maturing treatment, so that the maturing speed can be increased, the maturing effect can be improved, and the content of nutrient substances in compost is increased.

The invention discloses a bagasse filter mud composting method based on synergistic interaction of sargassum fusiforme and a complex microbial inoculant. The method comprises the following steps: mixing a sugar manufacturing byproduct bagasse and filter mud according to a certain dry weight ratio, and adjusting the moisture and carbon nitrogen ratio; synchronously adding sargassum fusiforme powder and a compound microbial agent, wherein the microbial agent is prepared by compounding bacillus licheniformis and phanerochaete chrysosporium; the high-temperature period is maintained for not less than 7 days, the total fermentation period is about 45 days, and the matured compost and the fertilizer thereof are prepared. The sargassum fusiforme is used as a natural conditioner, so that the composition of nutrient substances can be optimized, nitrogen and water are preserved, and heavy metals are passivated; and the lignocellulose is specifically degraded by the complex microbial inoculants. Through the synergistic effect of the bagasse and the filter mud, the high-temperature period of the compost is remarkably prolonged, the nutrient loss is reduced, the decomposition degree and the environmental safety of a compost product are improved, and an efficient and environment-friendly new way is provided for high-value resource utilization of the bagasse and the filter mud.

The invention discloses a bacillus strain and application thereof, the bacillus strain comprises bacillus licheniformis X-Z9 and bacillus subtilis X-Z19, the bacillus licheniformis X-Z9 and the bacillus subtilis X-Z19 are both preserved in the China Center for Type Culture Collection, the preservation number of the bacillus licheniformis X-Z9 is CCTCC (China Center for Type Culture Collection): M 2025850, and the preservation number of the bacillus subtilis X-Z19 is CCTCC NO: M 2025851. The invention also develops a compound microbial agent based on the novel strain, and the microbial agent successfully solves the key problems of serious ammonia release, large nitrogen loss, long decomposition period and the like in kitchen waste aerobic composting (especially in combination with a film covering technology). The microbial inoculum is convenient to apply, and provides a powerful biotechnology support for efficient, environment-friendly and resourceful treatment of the kitchen garbage.

The invention discloses a decomposition preparation for coniferous forest plant residues and a preparation method and application thereof, and belongs to the technical field of microorganisms. The composting preparation for the coniferous forest plant residues comprises bacillus subtilis, bacillus licheniformis and Maribacter thermophilus, the coniferous forest plant residues, fresh chicken manure and peanut shells or corn stigma are used as main raw materials for composting, the aerobic bacillus mainly plays a key role in decomposing lignocellulose and releasing nutrients, and the aerobic bacillus mainly plays a key role in decomposing the lignocellulose and releasing the nutrients; the three strains are used as functional auxiliary strains, the three strains have a synergistic effect and jointly promote efficient and directional decomposition conversion of the refractory coniferous forest residues, and finally the decomposition preparation which is rich in active functional groups, has a porous structure and is high in adsorption capacity is prepared. The invention provides an innovative biological conversion path for high-value resource utilization of the coniferous forest waste.

The invention belongs to the technical field of agricultural fertilizers, and particularly relates to an amino acid water-soluble fertilizer containing chitosan oligosaccharide and salt-tolerant bacillus and a preparation method of the amino acid water-soluble fertilizer. According to the method, litopenaeus vannamei heads are used as raw materials, a refined solution is prepared through mixed acid pretreatment, double-enzyme fermentation and nanofiltration membrane desalination, and the refined solution is mixed with a salt-tolerant domesticated bacillus licheniformis / bacillus natto solution, citric acid-malic acid chelated medium elements and a protective agent. According to the method, by optimizing a pretreatment process and a chelating system, synergistic degradation of shrimp head protein and chitin is achieved, the prepared fertilizer has the compound functions of amino acid, chitosan oligosaccharide and a salt-tolerant microbial agent, the problems that a microbial agent in a traditional fertilizer is poor in salt tolerance and prone to precipitation are solved, and the method is suitable for facility agriculture and saline-alkali soil and has a wide application prospect. The crop stress resistance can be enhanced; the growth effect is improved.

The invention relates to the technical field of fertilizer preparation, and particularly discloses a biological organic fertilizer rich in deep sea enzymolysis fish protein and a preparation method of the biological organic fertilizer. The biological organic fertilizer is prepared from the following components in parts by weight: 35 to 45 parts of organic fertilizer (containing deep sea enzymolysis fish protein, tobacco powder, meat powder and bone meal), 6 to 15 parts of compound microorganism (such as bacillus licheniformis and the like), 5 to 10 parts of medium element fertilizer, 1 to 2 parts of trace element fertilizer, 0.1 to 1 part of xanthan gum and sulfur suspension, 30 to 35 parts of outer layer fertilizer (containing humic acid, urea and the like) and 0.1 to 0.5 part of anti-blocking agent. The preparation method comprises the following steps: separately fermenting microorganisms to prepare bacterial powder; mixing, crushing and granulating the raw materials to form master batches; spraying xanthan gum and sulfur suspension on the surface of the master batch to form a film; spraying the outer-layer fertilizer slurry for several times; and finally, an anti-blocking agent is sprayed, and air drying is conducted. The deep sea enzymolysis fish protein is taken as a core, the balance of quick-acting fertilizer efficiency and slow release is realized, the soil structure can be improved, the crop growth is promoted, the stress resistance and the fertilizer utilization rate are improved, the soil-borne diseases are inhibited, and the method has the advantages of high efficiency, environment friendliness, economy, practicability and the like.

The application discloses a humification preparation containing bacillus licheniformis and a preparation method and application thereof, and belongs to the technical field of microorganisms. The humification preparation comprises the bacillus licheniformis and thermus arciformis. The bacillus licheniformis is preserved in the China General Microbiological Culture Collection Center on June 27, 2025, and the address of the preservation center is No. 3, Xili Beichen, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from a coastal saline-alkali soil, has high-temperature resistance and salt tolerance, and can secrete protease and lipase. After the bacillus licheniformis and the thermus arciformis are mixed, the synergistic effect is obvious, duck-derived red mud can be effectively converted into humic acid, and resourceful high-value utilization of the duck-derived red mud is realized.

The invention belongs to the technical field of feed additives, and particularly relates to a feed additive for improving the production performance, egg quality and body health of laying hens and application of the feed additive. The feed additive is composed of mulberry leaf powder and bacillus licheniformis. The invention also provides a feed which is composed of a basic feed and the feed additive, each 1kg of the feed contains 1 * 10 < 10 > CFU of bacillus licheniformis, and the mass of the mulberry leaf powder is 9%-15% of the mass of the feed. Animal experiments prove that the feed additive disclosed by the invention has no obvious negative influence on the production performance and the body health of laying hens, and can also obviously improve the egg quality. The invention provides a feasible scheme for reduction and substitution of soybean meal and corn in livestock and poultry feed, and has important practical significance for relieving structural shortage of protein feed raw materials.

The invention relates to the technical field of microorganisms, in particular to bacillus licheniformis JSF-9 and a complex microbial inoculant and application thereof.The strain JSF-9 is obtained by being separated from healthy cattle rectum contents by a research group, and it is detected that the strain has the good beta-1, 4-glucanase producing capacity, and the beta-1, 4-glucanase producing capacity is high. The antibacterial effect on staphylococcus aureus, salmonella typhimurium and shigella flexneri is achieved; the strain has a good bagasse fermentation capability, for example, after the strain, bacillus altitudinis MLF-1 and bacillus safensis MLL-5 are prepared into a mixed microbial inoculum, the bagasse fermentation capability is better, and verification shows that the complex microbial inoculum can effectively inhibit the reproduction of harmful bacteria, so that the preservation period of fermented feed is prolonged, and the yield of the fermented feed is improved. Animal feeding experiments find that the nutritional value and the disease resistance of the complex microbial inoculant fermented bagasse are remarkably improved, 10-15% of the complex microbial inoculant fermented bagasse is added into basic ration, and the cost-reducing and effect-increasing effects are achieved for feeding Jersey cattle.

The invention relates to the technical field of microorganisms, in particular to bacillus licheniformis, a fermentation agent containing the bacillus licheniformis, a preparation method of the fermentation agent, organic fertilizer, a preparation method of the organic fertilizer and a planting method of crops. The preservation number of the bacillus licheniformis is GDMCC No: 66520. The strain is only added as a zymophyte to compost the agaricus bisporus residues, so that the quick decomposition of the agaricus bisporus residues can be realized. And the decomposed product has a good promotion effect on crops, especially tomatoes.

The invention belongs to the technical field of biological agriculture, and relates to bacillus licheniformis and application thereof. The strain disclosed by the invention has a good broad-spectrum antibacterial effect, has a good inhibition effect on various plant fungal pathogenic bacteria and bacterial pathogenic bacteria causing plant diseases, and particularly has a relatively good prevention and treatment effect on pumpkin anthracnose, and the prevention and treatment effect on the pumpkin anthracnose reaches 95% or above by using 10,000,000 cfu / mL of a microbial agent diluent; the prevention effect is obvious. The bacterial strain and the biological agent containing the bacterial strain can effectively overcome the defect that an existing product is single in control effect, and crop plant growth can be further promoted. And the bacillus licheniformis is collected from the natural environment, is safe, environment-friendly and pollution-free, and has a wide application prospect.

The invention relates to a preparation method and application of a gamma-PGA-rich feed additive for broiler chickens, and the technical scheme for solving the problems is as follows: screening bacillus licheniformis DY-0089 for high yield of gamma-PGA from fermented foods such as fermented soya beans, fermented bean curds and natto, inoculating the bacillus licheniformis DY-0089 on a solid fermentation culture medium, fermenting for 24-96 hours at 28-45 DEG C, drying in a drying oven at 55-70 DEG C, and crushing to obtain the gamma-PGA-rich feed additive for broiler chickens. And preparing the feed additive rich in gamma-PGA. Research finds that when 1% of the gamma-PGA-rich feed additive is fed to broilers, the immune function of the body can be enhanced, cecum microbiota can be improved, cecum metabonomics can be adjusted, then the growth performance and feed conversion rate of the broilers are improved, serum biochemical indexes, oxidation resistance, slaughter performance, pectoral muscle nutritional ingredients and the like of the broilers are basically not affected, and the feed additive is suitable for large-scale production. And a theoretical basis is provided for development and application of the gamma-PGA-rich feed additive in animal breeding, especially broiler breeding.