1781 results about "Lichen" patented technology

A building article incorporating a biocidal agent, such as copper oxine, that inhibits the growth of mold, fungi, algae, mildew, bacteria, lichen, and other undesirable biological growth is provided. The biocidal agent can be a biocide, fungicide, germicide, insecticide, mildewcide, or the like. The biocidal agent can be interspersed throughout the matrix of the article; applied as a surface treatment to the article; or applied as a treatment to the fibers reinforcing the article. The building article can include tile backer boards, decks, soffits, trims, decking, fencing, roofing, cladding, sheathing, and other products. The building article can also include a variety of different composite materials such as cement, gypsum, wood, and wood / polymer composites.

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention relates to a water improver with multiple active microbes and a preparation method thereof, which adopts two or a plurality of following microbial strains, comprising a bacillus licheniformis, a bacillus subtilis, a bacillus megaterium, a lactobacillus plantarum, a saccharomyces cerevisiae, a candida lipolytica, bacillus pumilus, bacillus cereus, a rhodopsedomnas palustris, an alcaligenes faecalis and an arthrobacter globiformis. After the femination of the microbial strains, the preparation of the liquid is that the single microzyme zymocyte liquid is mixed and stirred in proportion; or the mixed bacteria solution is concentrated; or the concentrated single microzyme zymocyte liquid is mixed and stirred according to the proportion of the single microzyme zymocyte liquid, and is prepared into a mix bacteria solution. The mix bacteria solution is mixed homogeniously with an added assistant agent. The total amount of the live bacteria within the warrenty period is 106 to 1010 per milliliter. The preparation of the solid inoculant is that the zymocyte liquid is dried after the absoption of the adsorbent on the zymocyte liquid, is prepared into a solid bacterial powder and mixed evenly with the added assistant agent. The total amount of the live bacteria within the warreny period ranges between 106 and 1011 per gram. The invention has multi-function and stable effect and is suitable for treating various water bodies.

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The invention relates to a micro-ecological preparation and the application thereof, in particular to a micro-ecological preparation containing a variety of probiotics and the application thereof. The micro-ecological preparation contains any three or four of the components including CGMCC No. 2383 bacillus licheniformis powder, bacillus subtilis powder, CGMCC No. 2386 enterococcus faecalis powder, lactobacillus acidophilus powder, and CGMCC No. 2388 saccharomyces cerevisiae. The micro-ecological preparation has content of live bacteria, has the adversity resistance such as gastric acid resistance, bile salt resistance, high-temperature resistance, common antibiotic resistance, and the like and the probiotic functions of producing acid and enzyme and resisting pathogenic bacteria. The variety and the proportion of the probiotics and carrier can be determined according to different kinds of animals and different animal growth phase. The micro-ecological preparation can improve the feed utilization efficiency, increase the yield of meat, eggs and milk, promote the growth of the animal, improve the immunity and the disease resistance of the animal, replace the antibiotic and improve the quality of the animal product.

A composite microbial preparation is prepared from hay bacillus (35-45%) lichen bacillus (25-35%) and thermofat bacillus (25-35%) through inclulating, culturing, proportional mixing, and culturing again. A microbial leaven is prepared from said composite microbial preparation through adsorption, press filter, breaking and baking. A microbial fertilizer is prepared from said composite microbial preparation, mud, peet, dung and dropping of chicken and livestock, distiller's grain, powered coal ash, straw or stalk, and city refuse through fermenting.

The invention discloses a functional microbial fertilizer and a production method thereof. The production method comprises the following steps: grinding 60%-70% of mushroom residue and 15%-25% of livestock manure to 2-3mm to obtain the major material by a grinder, regulating water content to be 60%-65%; adding composite fermenting agent consisting of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride in accordance with 50g-100g / T; stacking, fermenting and maturing for 15-30d, and turning 5-10 times during the process; evenly mixing the major material prepared from fermentation with auxiliary materials consisting of 6%-10% humic acid and 6%-10% rice hull; adding arbuscular mycorrhiza agent in accordance with 50g-100g / T; and sieving with a 3mm sieve to obtain the fertilizer. The invention provides an economic and effective technological approach for the resource recycling of agricultural wastes in China, and has important application prospect on promoting the development of green agriculture, controlling and reducing rural environmental pollution, improving rural eco-environment and other aspects.

In mesic environments, erosion control measures usually employ the establishment of vegetative cover by vascular plants in order to hold the soil in place. The current art often includes the application of seeds, chemical fertilizers, tackifiers, and mulches to promote the growth of vascular plants. However, arid environments so not support dense vegetative cover, but are instead dominated by photosynthetic microorganisms, primarily cyanobacteria and lichens. The cyanobacteria not only hold the soil in place, but also are the primary source of fixed nitrogen in arid environments. Disclosed herein, is a description of an apparatus and methods for the production and preservation of a photobiofertilizer as a means for repairing disturbed arid soils.

The invention discloses a fermented feed additive, a preparation method and application thereof. The fermented feed additive is prepared by fermenting mixed strains and mixed auxiliary materials consisting of monosodium glutamate protein, bean pulp, bran, cottonseed meal, corn fibers, rice bran and powdered rice hulls, wherein the mixed strains are obtained by mixing activated strains of lactobacillus acidophilus, lactobacillus planetarium, lactobacillus casei, saccharomyces cerevisiae, bacillus subtilis and bacillus licheniformis in a proportion of (1-5): (1-5): (1-5): (1-5): (1-5): (1-5); and the dosage of the fermented feed additive is 5 to 100 percent of the weight of the animal feed when used for the animal feed. According to the fermented feed additive, the bean pulp, the bran, the cottonseed meal and other raw auxiliary materials are sufficiently utilized, so that the food consumption in the animal feeding process is reduced; the fermented feed additive can be used to partiallyor completely replace the animal feed, and antibiotic medicaments can be reduced or prevented from being used; and no medicament is left, so that the feeding cost of farmers is reduced.

The invention relates to a complex microbial inoculum 707 and a preparation method and application thereof, and belongs to the technical field of biological materials of the application of plant biological regulators to crop planting. The preparation method of the complex microbial inoculum 707 comprises the following step of: performing the cultivation of one-level strains and second-level and third-level fermentation on strains comprising 45 to 55 percent of bacillus subtilis, 5 to 10 percent of bacillus licheniformis, 5 to 10 percent of bacillus megaterium, 5 to 10 percent of candida utilis, 5 to 10 percent of rhizobium japonicum, 5 to 10 percent of bacillus mucilaginosus and 5 to 10 percent of lactobacillus plantarum, so that the microbial content of fermentation liquor reaches 6-10*10<9> per milliliter to obtain the complex microbial inoculum 707. Through long-term field verification, the complex microbial inoculum 707 has the advantages of reasonable formula and stable effect, can promote the growth of crops, improve fruit quality, promote early ripening, increase yield, improve the utilization rate of fertilizers and improve the ecological environment of soil, and has certain effect on disease prevention and disease resistance.

The invention discloses a microbial fertilizer producing by using agricultural wastes and preparation thereof. Main materials are obtained by mixing and smashing 20%-40% oil sally bean dregs, 40%-60% crop straw and 15%-25% poultry excrement, moisture content achieves 60-65%, composite leaven is added according to 150g-200g / T to pile to be cone-shaped, yellow mud is used on the outside for sealing, a plastic film is used for covering in a sealing mode, after stacking, fermentation and digesting, and the composite leaven is composed of bacillus subtilis, bacillus licheniformis, trichoderma harzianum and trichoderma viride according to the mass ratio (W / W) of 1.5:1:1:2.5:4. Auxiliary materials made of 5%-10% mushroom dregs and 6%-10% rice husk ash are evenly mixed with fermented and prepared main materials, arbuscular mycorrhizal fungi agent are added according to 600-1000g / T for compounding, and the microbial fertilizer is prepared after passing a 3mm sieve. Good production and efficiency improvement is obtained through application, and the microbial fertilizer has good application value.

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g / l.

The invention relates to an agricultural microbial fertilizer and a preparation method and application thereof. The agricultural microbial fertilizer comprises a medium and thalli. The thalli comprise bacillus subtilis, Bacillus megaterium, Bacillus mucilaginosus, Bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, Bacillus mucilaginosus, Streptomyces jingyangensis, azotobacter vinelandii, bacillus coagulans, Aspergillus oryzae and paecilomyces lilacinus. The agricultural microbial fertilizer provided by the invention can improve soil, can effectively control part of plant diseases and insect pests and can supply nutrients to soil to effectively solve a long-term problem of soil hardening. In addition, the agricultural microbial fertilizer can dissolve phosphorus and potassium so as to affectively release phosphorus, potassium, etc. existing in the form of a chelate in the soil.

The invention relates to a formula and production method for preparing a peach wheat straw dye. The formula takes roses, lichenes, tulipa primulina, white alum, an iron ion compound solution and mineral water as main raw materials. The main process flow of the production method comprises the following steps: selecting materials; cutting the materials; drying; soaking; carrying out hot stewing; mixing; depositing; and dyeing to obtain a finished product. The formula and production method disclosed by the invention are used for preparing the peach wheat straw dye which is purely natural, pollution-free, green and environmental-friendly without toxic and side effects on human bodies and is prepared from pure natural plants.

The invention relates to a strain capable of degrading feather keratin efficiently and a screening method thereof. According to result of the analysis and identification of the sequence of 16SrDNA and the result of the identification of a Biolog system, F4 is determined to be Bacillus licheniformis and is named Bacillus licheniformis F4. The Bacillus licheniformis F4 was collected in China Center for Type Culture Collection with a collection number of CGMCC No.4229 on October 22th, 2010. The screening method comprises: primarily screening, separating, enriching, and secondarily screening to obtain the strain capable of degrading feather keratin efficiently. In the invention, the strain capable of degrading feather keratin efficiently is obtained by two steps of screenings under strict conditions, wherein primary screening conditions are a carbon source and a nitrogen source, and secondary screening conditions are complete degradation of feathers and measurement of enzymatic activity of keratin. The strain is inoculated into a fermentation culture medium containing complete feathers to perform fermentation culture for 60 hours, all barbules of the feathers are removed, the left feather stems and a major part of removed pinnules are degraded, and the feather removing and feather keratin degrading effects of the strain are obvious.

The invention relates to a synbiotics of bacillus licheniformis and oligosaccharide class prebiotics and a composite and a formulation thereof, being characterized in that the synbiotics comprises bacillus licheniformis medical live bacterial powder and the oligosaccharide class prebiotics, wherein the bacillus licheniformis medical live bacterial powder contains live bacteria about 30 billion / gram; the weight ratio of the bacillus licheniformis medical live bacterial powder to the oligosaccharide class prebiotics is 1:1 to 76; the weight ratio of the medical live bacterial powder of the composite, the oligosaccharide class prebiotics and auxiliary materials is as follows: 1.25-5 percent of the bacillus licheniformis medical live bacterial powder, 5-95 percent of the oligosaccharide class prebiotics, 20-50 percent of preferable oligosaccharide class prebiotics, 30-65 percent of diluting agents, 1-20 percent of bonding agents, 1-15 percent of disintegrating agents, 0.1-3 percent of lubricating agents, 1-10 percent of coating agents, 0.01-0.1 percent of flavoring agents, 0.0001-0.001 percent of coloring agents, and 0.1-15 percent of suspending agents. The synbiotics composite is prepared into oral common tablets, oral cavity disintegration tablets, dispersing tablets, enteric-coated tablets, granular formulation, enteric-coated granular formulation, capsules, enteric-coated capsules, dry supensoid agents and external tablets according to a conventional process; and an in vitro test shows that each formulation can promote the growth and the reproduction of the synbiotics of bacillus licheniformis, restrain the growth of harmful bacteria, enhance the immunizing power of parasitifers, reduce diarrhea and enhance the health care.

The invention discloses a multifunctional microorganism soil repairing agent as well as preparation and application thereof and belongs to the fields of biological techniques and environment protection. The multifunctional microorganism soil repairing agent is made of the following raw materials in parts by weight through uniform mixing: 30-60 parts of nano chitosan, 10-20 parts of humic acid, 10-20 parts of nano charcoal and 20-30 parts of a composite microbial agent, wherein the composite microbial agent is prepared from fresh benzene bacillus, B.sonorensis, lysinibacillus sphaericus, bacillus licheniformis, bacillus circulans, bBacillus thuringiensis, achromobacter denitrificans, saccharothrix australiensis, Rhodococcus zopfii, pseudonocardia sulfidoxydans and pseudonocardia dioxanivorans through purification, optimization, fermentation and drying dehydration. The multifunctional microorganism soil repairing agent disclosed by the invention is capable of replacing conventional physical and chemical repairing techniques, is capable of degrading complex pollutants, is wide in repairing range, is capable of preventing soil degradation and inhibiting diseases and insects in environments, and has the advantages of being good in repairing treatment effect, low in cost and economic and environment-friendly.

The invention relates to a method for fermentation production of a poultry bacterial agent by multi-bacteria miscible liquid, wherein aerobic bacteria, namely Bacillus subtilis, Bacillus licheniformis, bacillus natto, beer yeast, Aspergillus niger and Aspergillus oryzae are cultured in a shaking table according to different culture mediums, so as to culture a mother seed solution; simultaneously Lactobacillus acidophilus, bifidobacteria and enterococcus faecalis are subjected to anaerobic culture by utilization of Kille flasks, and a mother bacterial solution of photosynthetic bacteria is cultured by a Kille flask under the condition of illumination; the prior stock solution is inoculated into a seed tank for anaerobic culture and fermentation according to 4 percent of the inoculum concentration, and the fermentation time is between 48 and 60 hours; and the cultured seed liquid is inoculated into a productive tank for anaerobic culture and fermentation according to 10 percent of the inoculum concentration, the fermentation time is between 60 and 72 hours, and the fermentation end point is reached when the pH value is reduced to 4.0. The method has the advantages that the microscopic examination viable count of the bacterial agent is more than 5 billion per milliliter, so that the bacterial agent is safe and nontoxic, thereby not only improving the disease resistance of poultry but also promoting the quick growth of the poultry, reducing the feed-meat ratio and improving the quality of meat, eggs and milk.

A composition is provided that includes Bacillus subtilis 2084 (NRRL B-50013) or a strain having all of the identifying characteristics of the Bacillus subtilis 2084 (NRRL B-50013), B. subtilis 27 (NRRL B-50105) or a strain having all of the identifying characteristics of the B. subtilis 27 (NRRL B-50105), and B. licheniformis 21 (NRRL B-50134) or a strain having all of the identifying characteristics of the B. licheniformis 21 (NRRL B-50134). Animal bedding that includes the Bacillus strains is also provided, as well as a method of making the animal bedding. Also provided are methods of controlling odors from animal waste. A method of making a composition including the Bacillus strains is also provided.

The invention relates to a leaven dedicated for fermenting bed of microorganism engineering constituting in weight ratio: Lactobacillus of 15%-25%, enterococcus of 15-30%, yeast of 5-15%, bacillus subtilis of 10-30%, Bacillus licheniformis of 5-20%, Lactobacillus sporogenes of 5-20%; total live microbe being 5-10 hundred million / g. The leaven of the invention is composed of biotic bacteria with strong activity, and has definite composition and mechanism, and stable product quality, and good health effect for improving immunity of the animal and preventing disease, strong degradation ability for pig manure; the leaven can not degrade material of fermenting bed pad, can prolongs life of fermenting bed; the fermenting bed can be conveniently and simply manufactured, which saves more than 80% of labor.

The invention discloses a clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system-based bacillus licheniformis genome editing vector. A preparation method of the bacillus licheniformis genome editing vector comprises the steps of integrating a Cas9 protein expression cassette and a small guide ribonucleic acid (sgRNA) scaffold expression cassette into a starting vector pHY xyl to obtain pHY-Cas9-sgRNA scaffold; then, integrating any one of multiple sgRNA fragments, which are designed and synthesized by taking amyL as a target site, into the pHY-Cas9-sgRNA scaffold to obtain the bacillus licheniformis genome editing vector. The CRISPR-Cas9 system-based bacillus licheniformis genome editing vector can greatly improve the current situation that at present, the gene editing of bacillus licheniformis is difficult, and obviously increases the gene editing efficiency.

The invention discloses a preparation method and application of a composite microbial feed additive, belonging to the technical field of microbial engineering. The composite microbial feed additive provided in the invention comprises a live Clostridium butyricum preparation and a live Bacillus licheniformis preparation, wherein the live Clostridium butyricum preparation is obtained by the steps of seed culturing in triangular flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch, and the live Bacillus licheniformis preparation is obtained by the steps of strain culturing on an inclined plane, culturing in eggplant-like flasks, culturing in a seeding tank, culturing in a fermentation cylinder, bacterial sludge collecting by centrifugation, mixing with calcium carbonate, drying at low temperature, crushing, sieving and mixing with the accessory starch. The composite microbial feed additive is obtained by evenly mixing the live Clostridium butyricum preparation and the live Bacillus licheniformis preparation according to a mass ratio of 1:1. The composite microbial feed additive can substantially raise the utilization rate of a feed, significantly reduce cultivation cost and increase economic benefits.

The invention relates to an oral traditional Chinese medicine for treating skin diseases. The oral traditional Chinese medicine is prepared from radix lithospermi, rhizoma smilacis glabrae, fructus gardeniae, cortex dictamni, common peony roots, radix scrophulariae, rehmanniae radix, isatis indigotica forts, radix sophorae flavescentis, lalang grass rhizome, flos sophorae, radix sileris, puncturevine caltrop fruits, red-rooted salvia roots, ligusticum wallichii, large-leaved gentian, processed polygonum multiflorum, Chinese angelica, white paeony roots and licorice roots. The oral traditional Chinese medicine is a pure traditional Chinese medicine prescription, reduces the recurrence rate of psoriasis and obviously improves the cure rate. In the invention, by mixing the Chinese angelica, the white paeony roots, the isatis indigotica forts and the licorice roots for use, the organism immunity can be enhanced greatly and the problem of recurrence of the psoriasis due to cold and pharyngitis can be solved. The oral traditional Chinese medicine has high effective rate to the psoriasis more than 98 percent and also has 100 percent cure rate to conventional skin diseases such as acne, cutaneous pruritus, pityriasis rosea, eczema, lichen planus, neurodermatitis, urticaria, seborrheic dermatitis, and the like.

A fast-growing plant planting, shaping, carbon sequestration and comprehensive utilizing method includes: (1) planting and / or culturing fast-growing woody, herbal, algae, lichen or moss plants in a chosen land area or water body area; (2) performing cutting, harvesting or gathering when the fast-growing woody, herbal, algae, lichen or moss plants grow to the height or dimension suitable for cutting, harvesting or gathering after a period of time; (3) drying the cut, harvested or gathered fast-growing woody, herbal, algae, lichen or moss plants; (4) processing and shaping the dried fast-growing woody, herbal, algae, lichen or moss plants; (5) transporting the dried and formed biomass to a storage place for storing; and (6) comprehensively utilizing the dried fast-growing woody, herbal, algae, lichen or moss plants.

The invention provides a method through utilization of fast-growing herbaceous plants, of fast-growing alga, or lichen or moss to achieve carbon sequestration. The method includes a first step of planting and / or cultivating the fast-growing herbaceous plants, the fast-growing alga, the lichen or the moss in a selected land area or in a water area, a second step of carrying out harvesting or collecting when the fast-growing herbaceous plants, the fast-growing alga, the lichen or the moss grows to the appropriate height or the appropriate size after a period of time, a third step of carrying out drying on the harvested or collected plants, preferably, carrying out natural drying such as sun drying or air drying to obtain the dry plants, the dry alga, the dry lichen or the dry moss, a fourth step of transporting the dry plants, the dry alga, the dry lichen or the dry moss to a landfill and carrying out landfill, a fifth step of covering a layer of common soil on the final filled plants after the landfill is completed, and a sixth step of recovering vegetations in the landfill.

The invention discloses a farm fertilizer produced by biologic technology, in particular a decomposing agent for rapidly decomposing straws at low cost, the mixture ratio of which is aerobic bacillus subtilis Cohn 20%, Bacillus licheniformis 10%, aspergillus flavus 20%, chaetomiaceae 20%, absidia corymbifera 20%, and saccharomycetes 10%. The production procedures are: extracting different beneficial decomposed bacteria from organics from different environments, purifying, rejuvenating, breeding and culturing; inoculating the cultured bacterial strains into disinfected solid culture medium; fermenting for 4-5 days under 25-50 DEG C, airing dry so that the residual moisture in the bacterial strains is 25%; mixing, agitating evenly and packing the aired solid bacterial strains into solid decomposing agent. As the invention is made by using a unique optimization and combination of aerobic bacterial group and a high and new technology and production process, the invention is a pure biologic product, is free from smell and harm for making organic fertilizer by effectively decomposing wastes in the countryside, and the fermenting speed is high, the cost is low, and the source of raw material is wide. The invention has improved the soil structure and reduces environmental pollution.

An aqueous composition is disclosed, comprising at least one strain of Class 1 Bacillus bacterial spores, selected from Bacillus Cirulans, Bacillus Megaterium, Bacillus Lichenforms, Bacillus Pumilus, Bacillus Sphaericus, Bacillus Subtilis sub species 10144 and Bacillus Subtilis sub species 8646, a terpene and one or more surfactants. The terpene is preferably d-limonene. A method for cleaning surfaces is also disclosed, which method comprises deploying an aqueous composition comprising at least one strain of Class 1 Bacillus bacterial spores, selected from Bacillus Cirulans, Bacillus Megaterium, Bacillus Lichenforms, Bacillus Pumilus, Bacillus Sphaericus, Bacillus Subtilis sub species 10144 and Bacillus Subtilis sub species 8646, a terpene and one or more surfactants to a surface.

The invention relates to a composite microbial preparation for treating a black and odorous river and a preparation method thereof. The composite microbial preparation is made from: Thiobacillus denitrificans powder 40-60 parts, composite Bacillus powder 10-40 parts and composite Saccharomyces cerevisiae powder 10-40 parts. Strains for degrading sulfides and organics in black and odorous sediment and suitable for growing in the black and odorous sediment are specifically screened, the combination of Thiobacillus denitrificans efficient in degrading hydrogen sulfide and removing nitrogen, Bacillus amyloliquefaciens capable of generating rich enzymes such as protease, amylase and cellulase, Saccharomyces cerevisiae, Candida utilis and facultative anaerobic Bacillus licheniformis enables the preparation to quickly improve the water quality of the black and odorous river and remove sediment blackness and odor.

The present invention relates to an isolated polynucleotide of the complete chromosome of Bacillus licheniformis. The present invention also relates to isolated genes of the chromosome of Bacillus licheniformis which encode biologically active substances and to nucleic acid constructs, vectors, and host cells comprising the genes as well as methods for producing biologically active substances encoded by the genes and to methods of using the isolated genes of the complete chromosome of Bacillus licheniformis.

The invention discloses bacillus licheniformis and application thereof in the promotion of cellulose degradation. The invention provides the bacillus licheniformis J15CGMCC No.3905. Fermentation liquor obtained by fermenting the bacillus licheniformis J15 is the cellulose degradation enzymic preparation of the invention. The bacillus licheniformis J15 can be used for degrading cellulose and preparing compost. By degrading straws with cellulose decomposition bacteria, the bacillus licheniformis has the advantages of improving the nutritive value and the utilization value of the straw, along with high nutritive value, reproducibility, environmental friendliness, less energy consumption and the like. By inoculating the cellulose decomposition bacteria to the high temperature compost, cellulase content in the composting process can be obviously increased, the degradation of the cellulose and the decomposition of the compost can be promoted and the quality of the compost can be improved.