358 results about "Lichen" patented technology

The invention relates to the technical field of soil improvement, and discloses a soil improvement repairing agent and a preparation method thereof. The preparation method comprises the following steps: adding biochar, modified eugenol, modified benzothiazolyl pyridine, bacillus licheniformis and diatomite into a mixer, mixing and stirring, extruding and granulating in a granulator, and drying to obtain the soil improvement repairing agent. Eugenol can destroy a membrane structure and denature protein, so that a bacteriostatic effect is achieved, and a healthy soil microenvironment is provided for crops; the quaternary ammonium salt can destroy the membrane structure of pathogenic microorganisms, plays a role in killing the microorganisms, can improve the antibacterial effect of the remediation agent, and prevents and treats spread diseases of soil; nitrogen atoms and sulfur atoms in benzothiazolyl pyridine molecules can coordinate with free heavy metal ions in the soil, the heavy metal adsorption effect of the remediation agent is improved, the production value of the soil is gradually restored, meanwhile, boric acid capable of being absorbed by plants can be released through hydrolysis of pinacol ester, and the fertility of the soil is improved.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention discloses a composition containing cactus fermentation liquor and a preparation method and application of the composition in cosmetics, the cosmetic composition containing the cactus fermentation liquor comprises the cactus fermentation liquor, Ectoine and sodium hyaluronate according to a specific proportion, the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on succulent stems of cactus, and the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on the succulent stems of the cactus. Sequentially inoculating bacillus licheniformis and lactobacillus plantarum for sequential fermentation; and finally, purifying and enriching by ultrafiltration and nanofiltration two-stage membrane separation technologies. A product rich in small molecule active peptide and specific organic acid is obtained through biotransformation process combination, so that the final cosmetic composition has remarkable skin barrier repairing and anti-oxidation effects.

The invention discloses a probiotic animal feed and a preparation method thereof, and belongs to the technical field of animal feeds, the probiotic animal feed comprises the following components by mass: 40-55 parts of caragana microphylla powder, 20-30 parts of fermented soybean meal, 3-5 parts of a composite probiotic preparation, 5-10 parts of a medicinal and edible additive, and 2-3 parts of a mineral premix. According to the composite probiotic preparation, bacillus licheniformis, pediococcus pentosaceus and saccharomyces cerevisiae boulardii synergistically improve the fiber decomposition efficiency, inhibit pathogenic bacteria and reduce ammonia nitrogen emission. The medicinal and edible additive is of a multi-layer micro-capsule structure, the outer layer is carboxymethyl chitosan and copper gluconate modified nano zeolite, active substances in the middle layer are rapidly released through rumen pH response, and the middle layer contains a yucca extract, a liquorice extract and other components and can inhibit urease activity and ammonia-producing bacteria; in the inner layer, a nano-selenium-vitamin E compound, curcumin and the like are slowly released through sodium alginate-pectin gel, so that intestinal metabolism is optimized. The process of pretreating caragana microphylla through steam explosion and performing solid-state fermentation on soybean meal through aspergillus oryzae is utilized, so that grain-free production is realized.

The invention provides a 2-succinyl-5-enol acetonyl-6-hydroxy-3-cyclohexene-1-carboxylic acid (SEPHCHC) synthase mutant, which is characterized in that on the basis of a bacillus subtilis SEPHCHC synthase gene MenD, 12 SEPHCHC synthase mutants are obtained by virtue of a site-specific mutagenesis PCR (Polymerase Chain Reaction) technology, and the SEPHCHC synthase gene MenD is used as a base. Recombinant bacteria of bacillus subtilis or bacillus natto or bacillus licheniformis or bacillus amyloliquefaciens for expressing the mutant are respectively constructed, and the yield of heptaene menadione fermented by the recombinant bacteria is 74%-203% of the yield of heptaene menadione fermented by the recombinant bacteria of bacillus subtilis for expressing wild type SEPHCHC synthase MenD.

The invention belongs to the technical field of agricultural microbial control, and particularly relates to a growth-promoting and disease-preventing compound microbial agent and a preparation method and application thereof, and the compound microbial agent specifically comprises microbial powder, a growth auxiliary material and a drying agent; the microorganisms comprise vermiform yellow, trichoderma harzianum, bacillus velezensis, bacillus licheniformis and brevibacillus laterosporus. According to the compound microbial agent, through the synergistic effect of multiple strains, the control effect of the compound microbial agent on verticillium dahliae is enhanced, and the defects that a single biocontrol bacterium is single in biocontrol mechanism, poor in environment adaptability, incapable of being colonized in soil for a long time and unstable in control effect are overcome; the compound microbial agent can increase the yield of cotton and pepper and improve the quality of cotton fibers, and provides scientific and technological support and decision service for realizing green, environment-friendly, cost-saving and efficiency-increasing modern agriculture.

The invention discloses a method for improving uranium immobilization by bacillus by using an extracellular polymeric substance, and belongs to the field of environmental engineering, the bacillus licheniformis X-113 is separated from a uranium-polluted environment and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 20251207, and the bacillus licheniformis X-113 is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 20251207. By optimizing the EPS culture conditions, the fixing capacity of the EPS-Bacillus sp.X-113 on uranium is remarkably enhanced compared with that of Without EPS-Bacillus sp.X-113, and the fixing capacity of the EPS-Bacillus sp.X-113 on uranium is remarkably enhanced compared with that of Without EPS-Bacillus sp.X-113. And numerous functional groups in EPS, such as carboxyl, hydroxyl, amino and the like, can be subjected to complexation, ion exchange and other reactions with uranium ions to fix the uranium ions, so that the uranium fixation of the bacillus is greatly improved.

The invention discloses a bagasse filter mud composting method based on synergistic interaction of sargassum fusiforme and a complex microbial inoculant. The method comprises the following steps: mixing a sugar manufacturing byproduct bagasse and filter mud according to a certain dry weight ratio, and adjusting the moisture and carbon nitrogen ratio; synchronously adding sargassum fusiforme powder and a compound microbial agent, wherein the microbial agent is prepared by compounding bacillus licheniformis and phanerochaete chrysosporium; the high-temperature period is maintained for not less than 7 days, the total fermentation period is about 45 days, and the matured compost and the fertilizer thereof are prepared. The sargassum fusiforme is used as a natural conditioner, so that the composition of nutrient substances can be optimized, nitrogen and water are preserved, and heavy metals are passivated; and the lignocellulose is specifically degraded by the complex microbial inoculants. Through the synergistic effect of the bagasse and the filter mud, the high-temperature period of the compost is remarkably prolonged, the nutrient loss is reduced, the decomposition degree and the environmental safety of a compost product are improved, and an efficient and environment-friendly new way is provided for high-value resource utilization of the bagasse and the filter mud.

The invention discloses a decomposition preparation for coniferous forest plant residues and a preparation method and application thereof, and belongs to the technical field of microorganisms. The composting preparation for the coniferous forest plant residues comprises bacillus subtilis, bacillus licheniformis and Maribacter thermophilus, the coniferous forest plant residues, fresh chicken manure and peanut shells or corn stigma are used as main raw materials for composting, the aerobic bacillus mainly plays a key role in decomposing lignocellulose and releasing nutrients, and the aerobic bacillus mainly plays a key role in decomposing the lignocellulose and releasing the nutrients; the three strains are used as functional auxiliary strains, the three strains have a synergistic effect and jointly promote efficient and directional decomposition conversion of the refractory coniferous forest residues, and finally the decomposition preparation which is rich in active functional groups, has a porous structure and is high in adsorption capacity is prepared. The invention provides an innovative biological conversion path for high-value resource utilization of the coniferous forest waste.

The invention belongs to the technical field of agricultural fertilizers, and particularly relates to an amino acid water-soluble fertilizer containing chitosan oligosaccharide and salt-tolerant bacillus and a preparation method of the amino acid water-soluble fertilizer. According to the method, litopenaeus vannamei heads are used as raw materials, a refined solution is prepared through mixed acid pretreatment, double-enzyme fermentation and nanofiltration membrane desalination, and the refined solution is mixed with a salt-tolerant domesticated bacillus licheniformis / bacillus natto solution, citric acid-malic acid chelated medium elements and a protective agent. According to the method, by optimizing a pretreatment process and a chelating system, synergistic degradation of shrimp head protein and chitin is achieved, the prepared fertilizer has the compound functions of amino acid, chitosan oligosaccharide and a salt-tolerant microbial agent, the problems that a microbial agent in a traditional fertilizer is poor in salt tolerance and prone to precipitation are solved, and the method is suitable for facility agriculture and saline-alkali soil and has a wide application prospect. The crop stress resistance can be enhanced; the growth effect is improved.

The invention relates to the technical field of fertilizer preparation, and particularly discloses a biological organic fertilizer rich in deep sea enzymolysis fish protein and a preparation method of the biological organic fertilizer. The biological organic fertilizer is prepared from the following components in parts by weight: 35 to 45 parts of organic fertilizer (containing deep sea enzymolysis fish protein, tobacco powder, meat powder and bone meal), 6 to 15 parts of compound microorganism (such as bacillus licheniformis and the like), 5 to 10 parts of medium element fertilizer, 1 to 2 parts of trace element fertilizer, 0.1 to 1 part of xanthan gum and sulfur suspension, 30 to 35 parts of outer layer fertilizer (containing humic acid, urea and the like) and 0.1 to 0.5 part of anti-blocking agent. The preparation method comprises the following steps: separately fermenting microorganisms to prepare bacterial powder; mixing, crushing and granulating the raw materials to form master batches; spraying xanthan gum and sulfur suspension on the surface of the master batch to form a film; spraying the outer-layer fertilizer slurry for several times; and finally, an anti-blocking agent is sprayed, and air drying is conducted. The deep sea enzymolysis fish protein is taken as a core, the balance of quick-acting fertilizer efficiency and slow release is realized, the soil structure can be improved, the crop growth is promoted, the stress resistance and the fertilizer utilization rate are improved, the soil-borne diseases are inhibited, and the method has the advantages of high efficiency, environment friendliness, economy, practicability and the like.

The application discloses a humification preparation containing bacillus licheniformis and a preparation method and application thereof, and belongs to the technical field of microorganisms. The humification preparation comprises the bacillus licheniformis and thermus arciformis. The bacillus licheniformis is preserved in the China General Microbiological Culture Collection Center on June 27, 2025, and the address of the preservation center is No. 3, Xili Beichen, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from a coastal saline-alkali soil, has high-temperature resistance and salt tolerance, and can secrete protease and lipase. After the bacillus licheniformis and the thermus arciformis are mixed, the synergistic effect is obvious, duck-derived red mud can be effectively converted into humic acid, and resourceful high-value utilization of the duck-derived red mud is realized.

The invention belongs to the technical field of feed additives, and particularly relates to a feed additive for improving the production performance, egg quality and body health of laying hens and application of the feed additive. The feed additive is composed of mulberry leaf powder and bacillus licheniformis. The invention also provides a feed which is composed of a basic feed and the feed additive, each 1kg of the feed contains 1 * 10 < 10 > CFU of bacillus licheniformis, and the mass of the mulberry leaf powder is 9%-15% of the mass of the feed. Animal experiments prove that the feed additive disclosed by the invention has no obvious negative influence on the production performance and the body health of laying hens, and can also obviously improve the egg quality. The invention provides a feasible scheme for reduction and substitution of soybean meal and corn in livestock and poultry feed, and has important practical significance for relieving structural shortage of protein feed raw materials.

The invention relates to the technical field of microorganisms, in particular to bacillus licheniformis, a fermentation agent containing the bacillus licheniformis, a preparation method of the fermentation agent, organic fertilizer, a preparation method of the organic fertilizer and a planting method of crops. The preservation number of the bacillus licheniformis is GDMCC No: 66520. The strain is only added as a zymophyte to compost the agaricus bisporus residues, so that the quick decomposition of the agaricus bisporus residues can be realized. And the decomposed product has a good promotion effect on crops, especially tomatoes.

The invention provides a core-shell microsphere for feed detoxification and a preparation method and application thereof.The core-shell microsphere comprises a sclerotium layer, a magnetic shell layer and a catalytic layer from inside to outside, and the sclerotium layer is composed of bacillus licheniformis and lactobacillus rhamnosus composite flora and a chitosan-sodium tripolyphosphate semipermeable membrane wrapping thalli; the magnetic shell layer is a Fe3O4 nano-particle coating, and the catalytic layer is a porous Mn-Ce-O shell. The microspheres have the double functions of biodegradation and chemical catalysis, the internal flora can degrade various toxins, the external catalytic layer efficiently decomposes stubborn toxins, the magnetic layer realizes rapid magnetic separation and recovery, and the microspheres are suitable for removing mycotoxins from feed raw materials such as corn and soybean meal.

The invention discloses a modifier for reducing wine grape soil obstacles and a preparation method and application thereof, and belongs to the technical field of soil improvement. According to the invention, the specific complex microbial inoculant composed of bacillus subtilis, bacillus licheniformis and trichoderma harzianum is adopted to ferment the grape pomace, antibacterial substances and organic acids can be generated, and saline-alkaline obstacles of soil can be relieved; the fermentation product contains a large amount of bacillus subtilis, bacillus licheniformis and trichoderma harzianum, and can occupy the ecological niche of the root system through competition, optimize the rhizosphere environment and improve the biological enzyme activity of soil. Through the synergistic effect with the decomposed straw, the humic acid and the furfural residues, the rhizosphere microenvironment can be remarkably improved, decomposition and nutrient conversion of organic substances in soil are accelerated, the soil fertility is improved, root systems can better extend and absorb nutrients, plant growth is promoted, and the yield and quality of fruits are improved.

The invention discloses a combined reagent for detecting fungus (1-3)-beta-D glucan, a kit and a detection method of fungus (1-3)-beta-D glucan, and belongs to the technical field of biomedical detection. The serum sample treatment liquid comprises a carbonate buffer solution with the pH value of 9.5-10.5, bacillus licheniformis protease, CHAPS, a surfactant and a preservative. The protein denaturing agent stop buffer comprises a phosphate buffer, a protease inhibitor, BSA and other components. Under the mild weak alkaline condition, the combined action of protease and a denaturant is utilized, a fungus (1-3)-beta-D glucan antigen-antibody compound in serum is efficiently dissociated, an antibody is irreversibly inactivated, and meanwhile the antigen activity is kept. The method has the advantages of simple operation, no need of heating, centrifugation or strong corrosive reagents, high safety, no precipitation and no gel of the treated sample, and suitableness for subsequent immunological detection. The invention further provides a detection kit containing the solution and a corresponding detection method, and the detection accuracy and efficiency are remarkably improved.

The invention discloses a preparation method and application of an anti-continuous cropping microbial fertilizer, and aims to solve the problems of aggravation of crop diseases and insect pests and reduction of yield and quality caused by continuous cropping planting. The preparation method comprises the following steps: activating and uniformly mixing bacillus subtilis and bacillus licheniformis, and adding an alpha-lactalbumin solution and a calcium chloride solution to prepare a bacillus aggregate; adding bentonite into the polytrehalose solution, and performing ultrasonic treatment on the mixture and the humic acid solution to prepare a modified polytrehalose-bentonite compound; uniformly mixing acremonium terricola, trichoderma harzianum and a bacillus aggregate, and adding a wall material solution to prepare a compound bacterium microcapsule preparation; and finally, mixing the microcapsules with the corn straw biochar, the chitosan oligosaccharide and the like to obtain a finished product. Through calcium ion induction and humic acid modified embedding, the strain stability and viable count are improved, soil organic matter and nitrogen phosphorus and potassium nutrients can be activated, pathogenic bacteria are inhibited, the crop rhizosphere environment is improved, the growth index and quality of red kidney beans and potatoes are effectively improved, and the method is suitable for continuous cropping resistant planting of crops.

The invention discloses a bacillus constitutive strong promoter and application thereof, and relates to the technical field of biology, and the nucleotide sequence of the promoter is SEQ ID NO: 2. The mRNA transcriptional level of the reporter gene is improved; the method is used for starting a target gene to efficiently express amylase in bacillus subtilis. The method is also used for starting a target gene to efficiently express alkaline protease in bacillus licheniformis. The novel constitutive promoter with enhanced transcriptional activity is developed by taking P43 as a starting template, and under the same host and culture conditions, the absolute level of the constitutive promoter for driving expression of a reporter gene or a target product is higher than that of the current P43 promoter, so that core power is provided for performance improvement of a bacillus cell factory.

A living paint comprises a living photosynthetic microorganism, binder, growth medium, and water. The microorganism may comprise algae, cyanobacteria, or lichens. The binder may be composed of primary

The present application relates to the field of oil production, discloses a microbial wax removal and prevention agent, and a preparation method and application thereof, the microbial wax removal and prevention agent comprises a nutrient solution and microorganisms; the microorganisms comprise Exiguobacterium aurantiacum, and at least one of Bacillus stearothermophilus, Pseudomonas schneideri, Bacillus licheniformis and Rhodococcus ruber.The microbial wax removal and prevention agent is suitable for both ordinary wax-containing oil well wax removal and prevention and high calcium and magnesium wax-containing oil reservoir, has efficient wax dissolving and prevention effect, and has a wide application range.

This invention belongs to the field of wastewater treatment technology, specifically relating to a method for treating wastewater from industrialized aquaculture. This invention combines physical, biological, and chemical treatment methods, enabling efficient wastewater treatment in a short time, reducing the footprint and operating costs of treatment equipment, and exhibiting high economic efficiency and practicality. The combined use of Spirulina with Bacillus licheniformis, Bacillus subtilis, and Micrococcus luteus forms a stable algae-bacteria symbiotic system in the aquatic environment, accelerating the removal of nitrogen and phosphorus from the aquaculture wastewater and effectively reducing the chemical oxygen demand (COD). After treatment using this method, the wastewater reuse rate is no less than 80%, and the treated wastewater quality meets national discharge standards.

This invention belongs to the field of resource utilization technology, specifically relating to a low-temperature composting self-heating start-up compound microbial agent, its preparation method, and its application. The low-temperature composting self-heating start-up compound microbial agent is composed of *Broodia buddingis* (…). Aureobasidium sp.X4-8) and Bacillus licheniformis ( Bacillus licheniformis The compound microbial agent (H-29) was prepared by combining two bacterial groups. The two bacterial groups exhibited dominance and functional complementarity at different temperature stages. When added at -30℃, the psychrophilic fungi initially "ignited" the heat, and as the compost temperature exceeded 40℃, the heat-resistant bacteria rapidly took over and released lignin peroxidase and manganese peroxidase, achieving continuous regulation of the composting system from initial heating and high-temperature maintenance to deep decomposition under low-temperature conditions. Aerobic composting of agricultural waste using the compound microbial agent resulted in a seed germination index (GI) ≥ 70% for the decomposed product, with all indicators meeting the NY / T 525-2021 standard.

This invention relates to a microbial mineralization composite material for inkjet 3D printing and its preparation method. The composite material comprises powder materials and a binder. The powder materials, by weight, include: 70-85 parts magnesium phosphate cement powder, 10-25 parts fine aggregate, 0.5-3 parts a porous protective carrier loaded with urease-producing Bacillus licheniformis spores, 0.05-0.5 parts a nutrient protectant, and 1-5 parts a calcium source. The binder, by weight, includes: 94-98 parts deionized water, urea, 0.02-0.2 parts nutrients, 0.01-0.5 parts a pH adjuster, 0.5-5 parts a viscosity adjuster, and 0.01-0.2 parts a surface tension adjuster. The concentration of urea in the binder is 0.02-0.10 mol / L. This invention solves the problems of existing inkjet 3D printing materials having internal pores, microcracks, and weak interlayer interfaces after molding, limiting the improvement of the mechanical properties of printed parts in the later stages, and the difficulty in adapting the microbial mineralization system to the inkjet 3D printing process.

The invention relates to the technical field of microbial fermentation and bioengineering, in particular to bacillus licheniformis and application thereof in production of acetoin and tetramethylpyrazine. The bacillus licheniformis with high acetoin yield is screened from a large number of strains. The yield of acetoin after the strain is fermented for 36 hours can reach 98 g / L and is far higher than that of other existing natural screened strains, the liquor yield is increased by 11% when the strain is used for liquor brewing, the content of tetramethylpyrazine in liquor is increased by 57%, and the quality of the liquor is obviously improved.

The invention relates to the field of polypeptides, in particular to a bacillus licheniformis source antibacterial peptide and application thereof. The bacillus licheniformis source antibacterial peptide is selected from at least one of the following components: A1) the amino acid sequence of the bacillus licheniformis source antibacterial peptide is MVFFKYKKKIRIRKR (SEQ ID NO.1); a2) the bacillus licheniformis source antibacterial peptide with the same function is obtained by connecting tag protein to the N end and / or the C end of A1), and the bacillus licheniformis source antibacterial peptide has good antibacterial activity and can be widely applied to preparation of crop antibacterial products or screening of new antibacterial plant varieties.

The invention relates to the technical field of agricultural biology, and discloses a traditional Chinese medicine microbial probiotic stock solution, the active component of the stock solution comprises a fermentation product obtained by fermenting a traditional Chinese medicine fermentation substrate with a compound microbial flora, and the compound microbial flora comprises bacillus licheniformis, bacillus subtilis, saccharomyces cerevisiae and lactobacillus plantarum; the carbon and nitrogen source comprises 10-20 parts of brown sugar, 5-15 parts of bran and 1-3 parts of peptone, multiple strains are coordinated and complemented, the survival rate and colonization ability of the strains are improved by matching with the traditional Chinese medicine fermentation substrate, and the problem of unstable effect is solved; the composite carbon and nitrogen source and the functional traditional Chinese medicine group promote growth of multi-element metabolites, and insufficient efficacy is avoided; the fertilizer is suitable for breeding and planting multiple scenes, can replace antibiotics, and improves the fertilizer efficiency and delays the pesticide effect; the staged fermentation stimulates the synergism of strains, increases the active components, guarantees the stable efficiency, and assists the large-scale application of green agriculture.

The present disclosure belongs relates to a 2709 alkali protease mutant modified based on molecular dynamics calculation and use thereof in liquid detergents. The dynamic structure feature of a 2709 alkali protease derived from Bacillus licheniformis are studied by using a molecular dynamics method through computer simulation, which is a unique effective method capable of studying a relationship between the structure of the 2709 alkali protease and molecular dynamics as well as an enzyme function in an atomic level. After molecular dynamics calculation, appropriate sites are selected for protein engineering to obtain the 2709 alkali protease mutant suitable for industrial application of detergents. The protease mutant has significantly improved enzyme activity under the alkaline condition, is better in stability, not only has good decontamination effect but also has good component compatibility with components in the detergent, and can be widely applied to the field of washing industry.

The invention discloses a compound microbial agent and application thereof, and belongs to the technical field of microbial agents. The method specifically comprises the following steps: adding clostridium butyricum fermentation centrifugate into a fermentation tank, adding a nitrogen source, a carbon source and inorganic salt into the fermentation tank, then adding a bacillus subtilis solution and a bacillus licheniformis solution, stirring at a certain temperature and a certain rotating speed, fermenting for a period of time, then adding a lactic acid bacteria solution, and fermenting for a period of time at a certain rotating speed; stirring and fermenting at a certain rotating speed at a certain temperature, detecting the pH value after fermenting for a period of time, and when the pH value is reduced to 4.3-4.5, obtaining the compound microbial agent. The prepared compound microbial agent can improve the saline-alkali soil, effectively improve the pH of the saline-alkali soil, consume soil salinity and improve the crop germination rate.