894 results about "Lichen" patented technology

The invention discloses a complex microbial inoculant for coupling straw decomposition and soil carbon sequestration and a preparation method thereof, the complex microbial inoculant can rapidly promote degradation of lignocellulose of straw in soil by compounding bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa and the like, provides a basic material energy source for activity of soil microorganisms, and has the advantages of simple preparation process and low cost. Meanwhile, corresponding microorganisms achieve long-term storage of organic carbon through humus stabilization and mineral substance combined carbon (MAOC) stabilization, the storage efficiency of the organic carbon is remarkably improved through nano Fe3O4, and under the synergistic effect of all strains in the complex microbial inoculant, the carbon loss of straw returning can be effectively reduced, the carbon immobilization capacity of soil is improved, and the soil quality is improved. The organic carbon content in the soil is increased.

The invention provides a southern red soil dry land deacidification soil conditioner and a preparation method thereof, and belongs to the technical field of soil conditioners. The preparation method comprises the following steps: embedding bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens to prepare bacterial microspheres, adding the bacterial microspheres into gel formed by carboxymethyl chitosan and silk fibroin peptide to prepare sustained-release bacterial microsphere gel, uniformly mixing the sustained-release bacterial microsphere gel with porous silicon dioxide loaded with basalt and charcoal, and drying to prepare the acid-reducing soil conditioner for the southern red soil dry land. The acid-reducing soil conditioner for the southern red soil dry land not only improves the pH value of the soil, but also can loosen the soil and improve the air permeability of the soil to a certain extent, and promotes the absorption of crops to moisture and nutrient elements by improving the physical and chemical properties of the soil, so that the normal growth of the crops is ensured, and the acid-reducing soil conditioner has a wide application prospect.

The invention relates to a complex microbial inoculant for preventing and treating citrus huanglongbing as well as a preparation method and application of the complex microbial inoculant. The compound bacterial agent for preventing and treating the citrus huanglongbing is prepared from the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. The compound bacterial agent for preventing and treating the citrus huanglongbing comprises the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. After the complex microbial inoculant is added, the content of callose in infected leaves can be effectively reduced, the self-resistance mechanism of plants is induced, the disease resistance is improved, and the complex microbial inoculant has an obvious inhibiting effect and a growth promoting effect on Candidatus Liberibacter asiaticum.

The invention relates to the technical field of fruit wine, in particular to herbal fresh fruit wine and a brewing process thereof. The preparation method comprises the following steps: juicing juicy peaches, apples and raspberries, and adding water for blending; the preparation method comprises the following steps of: adding cellulase and pectinase for primary enzymolysis, adding papain and bromelain for secondary enzymolysis, performing enzyme deactivation and sterilization, inoculating bacillus licheniformis CI CC 24553, lactobacillus plantarum CI CC 21794 and saccharomyces cerevisiae CI CC 1406 for fermentation, and performing post-treatment to prepare the herbal fresh fruit wine. The herbal fresh fruit wine prepared by the invention has the effects of excellent oxidation resistance, high total sugar content and excellent smell and taste, and has better use experience.

The invention discloses a decomposition preparation containing bacillus licheniformis as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The decomposition preparation provided by the invention comprises bacillus licheniformis and Thermus arcuatus. The bacillus licheniformis is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 27, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from coastal saline-alkali soil, has high temperature resistance and salt resistance, and can secrete protease and lipase at the same time. After the bacillus licheniformis and the Thermus arcuatus are mixed, the synergistic effect is remarkable, the duck-derived red mud is effectively promoted to be converted into humic acid, and high-value resource utilization of the duck-derived red mud is achieved.

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention belongs to the technical field of soil improvement, and relates to a soil conditioner for saline-alkali soil and a soil improvement method. The soil conditioner comprises vinegar residues, chitin, glucose, a composite biological preparation, a modified gel and a functional additive, the composite biological agent comprises bacillus subtilis powder, bacillus licheniformis powder, trichoderma spore powder, brown algae powder and light calcium carbonate; the functional auxiliary agent comprises modified bentonite, polyglutamic acid, pregelatinized starch and biochar potassium humate. Through the synergistic effect of multiple components and structural design optimization, systematic remediation of physical properties, chemical properties and biological activity of the saline-alkali soil is achieved. According to the technology, the limitation of single function of a traditional improvement measure is broken through, and the long-standing technical problems of return salt rebound, low fertility, microbial community imbalance and the like in saline-alkali soil treatment are effectively solved by constructing a multi-layer and multi-dimensional soil improvement system.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a comprehensive utilization method of corn processing waste resources, which comprises the following steps: mixing corn steep liquor with calcium hydroxide, phytase and aspergillus oryzae SLY-M-15 leavening, carrying out enzymolysis, carrying out primary fermentation with corn bran, bacillus licheniformis, bacillus subtilis and saccharomyces cerevisiae, and carrying out secondary fermentation with corn bran, bacillus licheniformis, bacillus subtilis and saccharomyces cerevisiae; finally, lactobacillus fermentum and lactobacillus casei are inoculated for secondary fermentation, so that comprehensive utilization of the corn bran and the corn steep liquor is realized, and the contents of phytic acid, sulfite, zearalenone and vomitoxin are reduced; when the obtained third treatment product is applied to animal feed, the metabolism of mycotoxin can be influenced, and the content of zearalenone and vomitoxin can be reduced; further, the average daily gain can be improved, and the material-to-weight ratio is reduced; slaughtering performance can also be improved, for example, carcass weight, semi-eviscerating slaughtering rate and full eviscerating slaughtering rate are improved; the meat quality can be improved, such as the pH value is increased, the steaming loss is reduced, the shearing force is reduced, and the crude fat content is reduced.

The invention relates to the technical field of microorganism application, in particular to bacillus velezensis, antibacterial lipopeptide and application of the bacillus velezensis and the antibacterial lipopeptide. The invention provides bacillus velezensis BGMW2 separated from a mangrove forest water area in Qinzhou city in Guangxi Zhuang Autonomous District, and the preservation number of the bacillus velezensis BGMW2 is GDMCC No: 64790. The strain BGMW2 has broad-spectrum antibacterial activity, and can significantly inhibit pathogenic bacteria such as escherichia coli, salmonella typhimurium, shigella, bacillus licheniformis and staphylococcus aureus. According to the invention, an antibacterial lipopeptide is separated from a metabolite of the strain BGMW2, and the antibacterial lipopeptide is separated, purified and identified as C12surfactin. The antibacterial effect of the C12surfactin is superior to that of a common bacteriostatic agent Nisin, and can be comparable with that of gentamicin sulfate. The C12surfactin also has the characteristic of stable antibacterial property at the temperature of between 40 and 80 DEG C and the pH value of between 2.0 and 12.0, and has hydrolase resistance and low hemolytic activity. The bacterial strain BGMW2 and a metabolite antibacterial lipopeptide thereof provided by the invention have important application prospects in the fields of food, pharmacy, disinfection, protection, health care products, cosmetics and the like.

According to the method, bacillus licheniformis bacterial liquid and calcium ion liquid are sequentially, slowly and evenly sprayed on the surface of a soil body, soil particles on the surface of a slope are cemented, a layer of calcium carbonate protection film is formed on the surface of the soil body, the anti-scouring capacity of the soil body can be effectively improved, flowing of the soil particles is reduced, and plant growth is protected; the adopted bacillus licheniformis can also increase the number of effective microbial communities in soil, improve the root system environment, promote plant growth, inhibit reproduction of harmful microorganisms, improve the granular structure of the soil, improve the soil, regulate the pH value of the soil, improve the water storage, energy storage and heat preservation of the soil, improve the disease resistance of crops and promote the maturity of plants; after vegetation grows, the effect of water and soil conservation can be improved, generated calcium carbonate can also bond soil particles, water and soil loss is reduced, bacillus licheniformis plays a dual role, multiple utilization of resources is achieved, and wide application prospects are achieved.

The invention discloses an organic fertilizer for planting local cereal crops in a plateau region and a preparation method thereof, and belongs to the technical field of agricultural planting. The organic fertilizer for planting local cereal crops in the plateau region is prepared from the following preparation raw materials: decomposed animal waste, vermiculite, humic acid, citric acid, fish meal, bone meal, straw, cake meal and a compound microbial agent, the carrier is prepared from decomposed animal waste, vermiculite, humic acid and citric acid, so that the mechanical strength and durability of the carrier can be improved, and a good physical state can be kept in the storage, transportation and use processes; through compound use of bacillus licheniformis, bacillus subtilis and bacillus laterosporus and adjustment of the dosage ratio of all strains and the viable count of the compound microbial agent, the soil micro-ecological environment can be improved, breeding of beneficial microorganisms in soil is promoted, growth of harmful bacteria is inhibited, and the soil fertility and the water and fertilizer retention capacity are improved; the fertilizer has a good promotion effect on the growth of spring wheat, oat and other plateau cereal crops.

The invention relates to a decomposition-promoting phosphorus-dissolving deodorizing microbial agent for composting and a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, paenibacillus zerzeri C-A and bacillus chestnut brown SC in a mass ratio of 1.5: (1-3): (1.5-4). The viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 1 * 10 < 9 > CFU / g. The preparation method comprises the following steps: (1) strain activation; (2) strain amplification culture; (3) bacterial powder preparation; and (4) strain compounding. The invention relates to application in clean composting of organic wastes. The invention has the following advantages: the three strains in the microbial inoculum do not generate antagonism, and can obviously accelerate the conversion of insoluble phosphorus, promote decomposition and improve the effect of removing NH3 and H2S.

The invention relates to the technical field of soil improvement, and discloses a soil improvement repairing agent and a preparation method thereof. The preparation method comprises the following steps: adding biochar, modified eugenol, modified benzothiazolyl pyridine, bacillus licheniformis and diatomite into a mixer, mixing and stirring, extruding and granulating in a granulator, and drying to obtain the soil improvement repairing agent. Eugenol can destroy a membrane structure and denature protein, so that a bacteriostatic effect is achieved, and a healthy soil microenvironment is provided for crops; the quaternary ammonium salt can destroy the membrane structure of pathogenic microorganisms, plays a role in killing the microorganisms, can improve the antibacterial effect of the remediation agent, and prevents and treats spread diseases of soil; nitrogen atoms and sulfur atoms in benzothiazolyl pyridine molecules can coordinate with free heavy metal ions in the soil, the heavy metal adsorption effect of the remediation agent is improved, the production value of the soil is gradually restored, meanwhile, boric acid capable of being absorbed by plants can be released through hydrolysis of pinacol ester, and the fertility of the soil is improved.

The invention discloses a coated slow-release solid water-soluble fertilizer as well as a preparation method and application thereof. The coated slow-release solid water-soluble fertilizer comprises the following components in parts by weight: 15-20 parts of a nitrogen source, 8-12 parts of a phosphorus source, 15-25 parts of a potassium source, 3-8 parts of humic acid and 10-15 parts of charcoal powder, the composite material comprises the following components in parts by weight: 0.5-1.5 parts of functional microorganisms, 1-2 parts of microbial metabolite, 2-5 parts of seaweed extract and 5-8 parts of chitosan-gelatin composite material. The functional microorganisms are bacillus subtilis, bacillus licheniformis and azospirillum which are adsorbed on the charcoal powder. The coated slow-release solid water-soluble fertilizer provided by the invention has the effects of supplying nutrients and preventing and treating soil-borne diseases, achieves multiple effects by one fertilizer, and can improve the crop nutrient utilization rate, inhibit the soil-borne diseases and promote the diversity of soil microorganisms.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention provides an environment-friendly microbial strain and a water treatment agent preparation process, and relates to the field of water treatment. The environment-friendly microbial strain is prepared from the following raw materials in parts by weight: 20 parts of nitrifying bacteria, 20 parts of denitrifying bacteria, 35 parts of bacillus and 25 parts of EM bacteria, the nitrifying bacteria comprise nitrosomas and nitrobacteria, and the denitrifying bacteria comprise nitrosomas and nitrobacteria. The denitrifying bacteria comprise denitrifying bacilli and sternobacter, the bacillus comprises bacillus subtilis and bacillus licheniformis, and the EM bacteria comprise rhodopseudomonas, lactococcus lactis and saccharomyces cerevisiae. Through flora screening and culture, the culture efficiency is improved, through a fermentation process, fermentation liquor overflow and insufficient dissolved oxygen caused by excessive foam are effectively prevented, the stability of the fermentation process is ensured, through preparation preparation, a good packaging material and a sealing technology, moisture absorption and pollution of a microbial inoculum are prevented, and the quality and safety of a product are ensured.

The invention discloses an extreme thermophilic charcoal complex microbial inoculant and application thereof in aerobic composting of pig manure, and belongs to the technical field of microorganisms. The invention firstly discloses a strain of thermophilic thermophilic bacteria N1209, and secondly discloses an extreme thermophilic biochar complex microbial inoculant, which comprises geobacillus thermoglucosidase BGSC 95A1, bacillus licheniformis ZR-1, the thermophilic thermophilic thermophilic bacteria N1209 and geobacillus stearothermophilus L5. The extremely thermophilic biochar complex microbial inoculant has the characteristic of high temperature resistance, compared with a single microbial inoculant, produced amylase, protease and lipase are higher in activity and show synergistic advantages, decomposition of pig manure can be remarkably promoted, the extremely thermophilic biochar complex microbial inoculant has advantages in the aspects of nitrogen element retention and CH4 and N2O emission reduction in the pig manure composting process, and the extremely thermophilic biochar complex microbial inoculant can be applied to the field of pig manure composting. And a new method is provided for treatment of livestock breeding wastes.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention belongs to the technical field of bioengineering, and particularly relates to an activity-improved tyrosinase mutant obtained by site-directed mutagenesis through an overlapping PCR technology, and preparation and application thereof. The tyrosinase mutant is obtained by mutation containing F337I / F352I on the basis of wild type tyrosinase as shown in SEQ ID NO.1, the enzyme activity of the tyrosinase mutant is obviously improved compared with that of the wild type tyrosinase, the F337I / F352I mutant gene is used for constructing a recombinant vector, and the recombinant vector is successfully expressed in bacillus subtilis WB600, bacillus amyloliquefaciens CGMCC No.11218 and bacillus licheniformis 2709, so that the tyrosinase mutant is obtained. The recombinant strain with improved enzyme production activity is further optimized through a fermentation process to obtain the novel tyrosinase.

The present invention relates to a probiotic, feed supplement or feed additive, feed premix or feed for animals of the family Suidae, said probiotic or animal feed comprising a strain of Weizmannia faecalis and a strain of Bacillus licheniformis.

The invention discloses a novel microbial sludge reduction inoculant and a preparation method and application thereof, and belongs to the technical field of microorganisms, the microbial sludge reduction inoculant comprises a composite inoculant and a biomass additive; the complex microbial inoculant is prepared from the following components in parts by weight: 10 to 12 parts of lactic acid bacteria, 18 to 22 parts of saccharomycetes, 6 to 8 parts of bacillus licheniformis, 12 to 16 parts of bacillus subtilis, 4 to 6 parts of bacillus amyloliquefaciens, 7 to 9 parts of methane sarcina and 3 to 5 parts of horsehair methane bacteria; the biomass additive is fruit peel waste. According to the method disclosed by the invention, a biodegradation system with high activity of various required enzymes can be formed by utilizing the mutual action of microbial flora, dead or aged thalli in the activated sludge can be effectively destroyed and decomposed, meanwhile, organic matters can be decomposed, the sludge is subjected to reduction treatment under the condition that the effluent quality is not influenced, the treatment cost is low, and the treatment effect is good. The method is green and pollution-free and worthy of popularization.

The invention discloses an efficient and safe lignocellulose degradation compound microbial agent and application thereof. The compound microbial agent is prepared from the following active ingredients: streptomyces microflavus, bacillus licheniformis, rhizobium and cellulomonas flavigena. The compound microbial flora is constructed by selecting safe and non-pathogenic microorganisms with lignocellulose degradation capability, and agricultural wastes such as straws, cottonseed hulls and the like can be effectively degraded. The complex microbial inoculant provided by the invention can be added into an edible fungus cultivation material to shorten the cultivation period, and can also be used in the aspects of preparation, composting and the like of a fermented straw feed. According to the method, efficient bioconversion of the crop waste rich in lignocellulose is facilitated, and development of circular agriculture is assisted.

The invention discloses a microbial compound feed for improving disease resistance of blue crabs and a preparation method of the microbial compound feed, and belongs to the technical field of feed preparation. The microbial compound feed comprises the following raw materials in parts by weight: 30-40 parts of fermented soybean meal, 20-25 parts of bran, 15-20 parts of fish meal, 5-8 parts of a compound microbial agent, 3-5 parts of algal polysaccharides, 2-3 parts of a radix astragali extract, 1-2 parts of chitosan, 2-3 parts of a mineral premix and 1-1.5 parts of compound vitamins, wherein the compound microbial agent comprises bacillus licheniformis, lactobacillus acidophilus and saccharomyces cerevisiae. The microbial compound feed disclosed by the invention comprehensively improves the disease resistance of the blue crabs and improves the survival rate through various ways, such as nutrition balance, immune regulation and intestinal health improvement, so that the breeding cost is reduced, and the income of farmers is increased.

The invention discloses a preparation method of a special microbial agent for preventing cotton verticillium wilt. The preparation method comprises the following steps: respectively preparing seed solutions; the preparation method comprises the following steps: preparing a substrate, inoculating the substrate with a microbial seed solution, fermenting, drying and crushing a fermentation culture to obtain microbial powder, and mixing the microbial powder with a growth auxiliary material and a drying agent to obtain the microbial agent. The microbial bacterial powder is prepared from bacillus subtilis HFN04, bacillus amyloliquefaciens, bacillus laterosporus, bacillus megaterium and bacillus licheniformis. The microbial agent containing the bacillus subtilis HFN04 can effectively prevent cotton verticillium wilt, and can promote the growth and development of cotton, improve the quality and increase the yield; the preparation method of the microbial agent is simple in process and easy to operate, and the prepared microbial agent has no pollution to the environment.

The invention relates to the technical field of microorganism application, and particularly discloses bacillus atrophaeus and application thereof, and a microbial agent for saline-alkali soil and application thereof. The preservation number of the bacillus atrophaeus is CGMCC (China General Microbiological Culture Collection Center) Meanwhile, the invention further provides a microbial agent prepared from the bacillus atrophaeus, bacillus subtilis, bacillus licheniformis and bacillus velezensis, the preservation number of the bacillus subtilis is CGMCC No.29774, and the preservation number of the bacillus licheniformis is CGMCC No.29774. The preservation number of the bacillus licheniformis is CGMCC (China General Microbiological Culture Collection Center) The preservation number of the bacillus velezensis is CGMCC (China General Microbiological Culture Collection Center) The microbial agent for saline-alkali soil provided by the invention can significantly improve the emergence rate of corn seeds, the plant height and the root fresh weight of corn plants under the condition of saline-alkali soil, and can effectively improve the yield of crops under severe conditions.

The present invention belongs to the technical field of microbial fermentation, and discloses the application of a compound bacterium agent of Lactobacillus rhamnosus and Bacillus licheniformis for reducing acids and higher alcohols, Lactobacillus rhamnosus, and in Baijiu. Lactobacillus rhamnosus L21 has a preservation number of: CGMCC NO. 32609, a preservation date of: November 13, 2024, and a preservation unit of: China General Microbiological Culture Collection Center. The use of the compound bacterium agent of Lactobacillus rhamnosus L21 and Bacillus licheniformis XDNZ_Bl_27 can effectively reduce organic acids such as lactic acid and propionic acid in the Baijiu fermentation system, and at the same time can also reduce the content of n-propanol in the fermented grains, which is beneficial to the regulation of acidity and higher alcohols in the process of Baijiu production.

The invention discloses a composition containing cactus fermentation liquor and a preparation method and application of the composition in cosmetics, the cosmetic composition containing the cactus fermentation liquor comprises the cactus fermentation liquor, Ectoine and sodium hyaluronate according to a specific proportion, the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on succulent stems of cactus, and the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on the succulent stems of the cactus. Sequentially inoculating bacillus licheniformis and lactobacillus plantarum for sequential fermentation; and finally, purifying and enriching by ultrafiltration and nanofiltration two-stage membrane separation technologies. A product rich in small molecule active peptide and specific organic acid is obtained through biotransformation process combination, so that the final cosmetic composition has remarkable skin barrier repairing and anti-oxidation effects.

The invention discloses a planting method for increasing soil organic matters and carbon sequestration, belongs to the technical field of ecological soil improvement, and provides a planting method for increasing soil organic matters and carbon sequestration, which comprises the following steps: applying a complex microbial inoculant on the surface of a planting land; planting an arbor, and then planting caragana twisting in planting gaps of the arbor; the composite microbial inoculant is prepared from bacillus cereus (bacillus cereus) and bacillus parlicheniformis (bacillus parlicheniformis), and the composite microbial inoculant is prepared from bacillus cereus (bacillus cereus) and bacillus parlicheniformis (bacillus parlicheniformis); the arbor is picea crassifolia, pinus sylvestris and pinus tabulaeformis. According to the method, picea crassifolia, pinus sylvestris and pinus tabulaeformis of different forest ages are planted in a mixed mode, and caragana twistable is matched, so that the effects of maximizing soil organic matter accumulation and enhancing carbon sequestration in the Gansu Xieu region are achieved.

The invention discloses a bacillus licheniformis strain and application thereof. Belongs to the technical field of probiotics. A strain of bacillus licheniformis is separated and identified through a traditional identification method and a molecular biological method, and the bacillus licheniformis is subjected to probiotic performance evaluation and animal in-vivo safety evaluation. Meanwhile, microbiology is combined with pathology and the like, so that mouse organ lesions caused by salmonella can be relieved by drenching the bacterium, and an antagonistic effect on salmonella in a mouse body can be generated. According to the invention, not only can the research on bacillus be expanded, but also a reference basis can be provided for developing and researching probiotic microecological preparations for promoting digestion and growth of animals, antagonizing pathogenic bacteria, regulating intestinal microecological environment and the like in the future.