195 results about "Lichen" patented technology

The invention discloses a bagasse filter mud composting method based on synergistic interaction of sargassum fusiforme and a complex microbial inoculant. The method comprises the following steps: mixing a sugar manufacturing byproduct bagasse and filter mud according to a certain dry weight ratio, and adjusting the moisture and carbon nitrogen ratio; synchronously adding sargassum fusiforme powder and a compound microbial agent, wherein the microbial agent is prepared by compounding bacillus licheniformis and phanerochaete chrysosporium; the high-temperature period is maintained for not less than 7 days, the total fermentation period is about 45 days, and the matured compost and the fertilizer thereof are prepared. The sargassum fusiforme is used as a natural conditioner, so that the composition of nutrient substances can be optimized, nitrogen and water are preserved, and heavy metals are passivated; and the lignocellulose is specifically degraded by the complex microbial inoculants. Through the synergistic effect of the bagasse and the filter mud, the high-temperature period of the compost is remarkably prolonged, the nutrient loss is reduced, the decomposition degree and the environmental safety of a compost product are improved, and an efficient and environment-friendly new way is provided for high-value resource utilization of the bagasse and the filter mud.

The invention discloses a decomposition preparation for coniferous forest plant residues and a preparation method and application thereof, and belongs to the technical field of microorganisms. The composting preparation for the coniferous forest plant residues comprises bacillus subtilis, bacillus licheniformis and Maribacter thermophilus, the coniferous forest plant residues, fresh chicken manure and peanut shells or corn stigma are used as main raw materials for composting, the aerobic bacillus mainly plays a key role in decomposing lignocellulose and releasing nutrients, and the aerobic bacillus mainly plays a key role in decomposing the lignocellulose and releasing the nutrients; the three strains are used as functional auxiliary strains, the three strains have a synergistic effect and jointly promote efficient and directional decomposition conversion of the refractory coniferous forest residues, and finally the decomposition preparation which is rich in active functional groups, has a porous structure and is high in adsorption capacity is prepared. The invention provides an innovative biological conversion path for high-value resource utilization of the coniferous forest waste.

The invention relates to the technical field of microorganisms, in particular to bacillus licheniformis, a fermentation agent containing the bacillus licheniformis, a preparation method of the fermentation agent, organic fertilizer, a preparation method of the organic fertilizer and a planting method of crops. The preservation number of the bacillus licheniformis is GDMCC No: 66520. The strain is only added as a zymophyte to compost the agaricus bisporus residues, so that the quick decomposition of the agaricus bisporus residues can be realized. And the decomposed product has a good promotion effect on crops, especially tomatoes.

The invention provides a core-shell microsphere for feed detoxification and a preparation method and application thereof.The core-shell microsphere comprises a sclerotium layer, a magnetic shell layer and a catalytic layer from inside to outside, and the sclerotium layer is composed of bacillus licheniformis and lactobacillus rhamnosus composite flora and a chitosan-sodium tripolyphosphate semipermeable membrane wrapping thalli; the magnetic shell layer is a Fe3O4 nano-particle coating, and the catalytic layer is a porous Mn-Ce-O shell. The microspheres have the double functions of biodegradation and chemical catalysis, the internal flora can degrade various toxins, the external catalytic layer efficiently decomposes stubborn toxins, the magnetic layer realizes rapid magnetic separation and recovery, and the microspheres are suitable for removing mycotoxins from feed raw materials such as corn and soybean meal.

The invention discloses a bacillus constitutive strong promoter and application thereof, and relates to the technical field of biology, and the nucleotide sequence of the promoter is SEQ ID NO: 2. The mRNA transcriptional level of the reporter gene is improved; the method is used for starting a target gene to efficiently express amylase in bacillus subtilis. The method is also used for starting a target gene to efficiently express alkaline protease in bacillus licheniformis. The novel constitutive promoter with enhanced transcriptional activity is developed by taking P43 as a starting template, and under the same host and culture conditions, the absolute level of the constitutive promoter for driving expression of a reporter gene or a target product is higher than that of the current P43 promoter, so that core power is provided for performance improvement of a bacillus cell factory.

A living paint comprises a living photosynthetic microorganism, binder, growth medium, and water. The microorganism may comprise algae, cyanobacteria, or lichens. The binder may be composed of primary

The present application relates to the field of oil production, discloses a microbial wax removal and prevention agent, and a preparation method and application thereof, the microbial wax removal and prevention agent comprises a nutrient solution and microorganisms; the microorganisms comprise Exiguobacterium aurantiacum, and at least one of Bacillus stearothermophilus, Pseudomonas schneideri, Bacillus licheniformis and Rhodococcus ruber.The microbial wax removal and prevention agent is suitable for both ordinary wax-containing oil well wax removal and prevention and high calcium and magnesium wax-containing oil reservoir, has efficient wax dissolving and prevention effect, and has a wide application range.

This invention relates to a microbial mineralization composite material for inkjet 3D printing and its preparation method. The composite material comprises powder materials and a binder. The powder materials, by weight, include: 70-85 parts magnesium phosphate cement powder, 10-25 parts fine aggregate, 0.5-3 parts a porous protective carrier loaded with urease-producing Bacillus licheniformis spores, 0.05-0.5 parts a nutrient protectant, and 1-5 parts a calcium source. The binder, by weight, includes: 94-98 parts deionized water, urea, 0.02-0.2 parts nutrients, 0.01-0.5 parts a pH adjuster, 0.5-5 parts a viscosity adjuster, and 0.01-0.2 parts a surface tension adjuster. The concentration of urea in the binder is 0.02-0.10 mol / L. This invention solves the problems of existing inkjet 3D printing materials having internal pores, microcracks, and weak interlayer interfaces after molding, limiting the improvement of the mechanical properties of printed parts in the later stages, and the difficulty in adapting the microbial mineralization system to the inkjet 3D printing process.

The invention relates to the technical field of microbial fermentation and bioengineering, in particular to bacillus licheniformis and application thereof in production of acetoin and tetramethylpyrazine. The bacillus licheniformis with high acetoin yield is screened from a large number of strains. The yield of acetoin after the strain is fermented for 36 hours can reach 98 g / L and is far higher than that of other existing natural screened strains, the liquor yield is increased by 11% when the strain is used for liquor brewing, the content of tetramethylpyrazine in liquor is increased by 57%, and the quality of the liquor is obviously improved.

The invention relates to the technical field of agricultural biology, and discloses a traditional Chinese medicine microbial probiotic stock solution, the active component of the stock solution comprises a fermentation product obtained by fermenting a traditional Chinese medicine fermentation substrate with a compound microbial flora, and the compound microbial flora comprises bacillus licheniformis, bacillus subtilis, saccharomyces cerevisiae and lactobacillus plantarum; the carbon and nitrogen source comprises 10-20 parts of brown sugar, 5-15 parts of bran and 1-3 parts of peptone, multiple strains are coordinated and complemented, the survival rate and colonization ability of the strains are improved by matching with the traditional Chinese medicine fermentation substrate, and the problem of unstable effect is solved; the composite carbon and nitrogen source and the functional traditional Chinese medicine group promote growth of multi-element metabolites, and insufficient efficacy is avoided; the fertilizer is suitable for breeding and planting multiple scenes, can replace antibiotics, and improves the fertilizer efficiency and delays the pesticide effect; the staged fermentation stimulates the synergism of strains, increases the active components, guarantees the stable efficiency, and assists the large-scale application of green agriculture.

The present disclosure belongs relates to a 2709 alkali protease mutant modified based on molecular dynamics calculation and use thereof in liquid detergents. The dynamic structure feature of a 2709 alkali protease derived from Bacillus licheniformis are studied by using a molecular dynamics method through computer simulation, which is a unique effective method capable of studying a relationship between the structure of the 2709 alkali protease and molecular dynamics as well as an enzyme function in an atomic level. After molecular dynamics calculation, appropriate sites are selected for protein engineering to obtain the 2709 alkali protease mutant suitable for industrial application of detergents. The protease mutant has significantly improved enzyme activity under the alkaline condition, is better in stability, not only has good decontamination effect but also has good component compatibility with components in the detergent, and can be widely applied to the field of washing industry.

The application discloses a bacillus licheniformis YL-001 with anti-inflammatory and antibacterial effects and an application thereof, and belongs to the technical field of biotechnology. The bacillus licheniformis YL-001 is separated from soil. The preservation number of the bacillus licheniformis is GDMCC No.66556. The metabolic product of the bacillus licheniformis YL-001 provided in the application has the abilities of significantly inhibiting secretion of inflammatory factors and growth of streptococcus pneumoniae and hemolytic streptococcus b, and can improve the total antioxidant capacity of serum. The strain has good biocompatibility and high safety, has important significance for development of anti-inflammatory or antibacterial drugs, health products and food, and is especially suitable for preparation of products with throat-protecting effects.

The invention provides multifunctional bacillus licheniformis as well as a derivative product and application thereof, and belongs to the technical field of microbial agents. A multifunctional bacillus licheniformis strain is separated and screened from a chicken manure fermentation pile in a chicken farm in Jianyang City in Sichuan province, is named as Bacillus licheniformis NCF and is preserved in the China Center for Type Culture Collection, the preservation address is Wuhan University, Wuhan, China, the number of the preservation center is CCTCC No.M 20242510, and the preservation time is November 11, 2024. The NCF bacterium has multiple functions, can be added into feed as an additive, helps animals to better digest and absorb nutritional ingredients in the feed, and increases the weight of the animals; organic matters in the excrement can be decomposed through high-temperature fermentation, the fermentation process is accelerated, and the composting fermentation period is shortened; the odor value and the ammonia gas concentration in different livestock and poultry manure / biogas slurry can be effectively reduced.

The present invention relates to new strains of Bacillus paralicheniformis with improved growth rates and plant growth promoting properties and new strains of Bacillus paralicheniformis with improved growth rates when compared to their parental strains from where they are derived.

The present application belongs to the technical field of microorganisms, and particularly relates to a bacillus licheniformis BL-C15 and application thereof. The present application provides a bacillus licheniformis BL-C15, which is characterized in that the bacillus licheniformis BL-C15 is preserved in the China General Microbiological Culture Collection Center on November 25, 2024, has a preservation number of CGMCC No. 32787, and has a preservation address of No. 3, Xili, Beichen, Beijing, China. The strain has the characteristics of high-alkali tolerance, salt tolerance and acid production, can effectively repair saline-alkali degraded soil, has a significant plant growth-promoting effect, and has important application value in the field of saline-alkali soil repair. The strain can also be used for preparing a microbial product for repairing saline-alkali soil.

This invention relates to the field of soil remediation technology, specifically disclosing a method for remediating heavy metal-contaminated soil using microbial agents. The invention uses hydroxyapatite as a carrier, utilizing its adsorption properties to load licorice extract onto its surface. To further improve the stability of the licorice extract, a carboxymethyl cellulose (CMC) solution is added. After stirring, mixing, and drying, the excellent film-forming properties of CMC help improve the stability of the licorice extract, while also increasing the specific surface area and adsorption sites of the hydroxyapatite. The introduction of licorice extract and CMC not only enhances the chelating ability of hydroxyapatite for heavy metals but also provides an additional carbon source for microorganisms, promoting their growth and reproduction. The metabolic activity of *Trichoderma solani* and *Bacillus licheniformis* is significantly enhanced, resulting in a substantial increase in remediation efficiency.

The invention discloses a method, a system, a storage medium and equipment for determining the occurrence time and frequency of debris flow in an unmanned area based on the size of lichen, and relates to the technical field of geological disaster monitoring and dating, and the method comprises the steps of S1, condition setting, S2, lichen data acquisition, S3, lichen growth rate fitting, and S4, time and frequency determination. The method is characterized in that the exposure time of lichens is calculated according to the average diameter of ground clothes and the actual growth rate of the lichens in combination with the initial diameter of the lichens, the occurrence time of debris flow is determined according to the exposure time of the ground clothes, and the occurrence frequency of the debris flow is obtained according to the occurrence time of the debris flow. The technical problem that the time and frequency of debris flow events in depopulated areas are difficult to determine is effectively solved.

The invention provides bacillus licheniformis capable of producing amylase, protease and cellulase and application of the bacillus licheniformis. The bacillus licheniformis is bacillus licheniformis FJAT-27214 and is preserved in the China General Microbiological Culture Collection Center on August 6, 2025, the preservation address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35536. The bacillus licheniformis disclosed by the invention can be used for simultaneously producing amylase, protease and cellulase at high yield; the bacillus subtilis has antibacterial activity to escherichia coli and has certain tolerance to artificial gastric juice, artificial intestinal juice and cholate, the survival rate in the artificial gastric juice is 58.11%, the survival rate in the artificial intestinal juice is 82.58%, and the survival rate in the cholate is 28.03%. When the strain fermentation liquor is used for pomace micro-storage, the content of crude protein and total sugar of pomace can be remarkably increased, meanwhile, the content of neutral detergent fiber and ammoniacal nitrogen is remarkably reduced, and an effective strain resource is provided for pomace micro-storage fermented feed.

The present application belongs to the field of biotechnology, and particularly relates to a preparation method of a taiwanofungus castaneus enzyme Trichoderma longibrachiatum and bacillus licheniformis Bacillus licheniformis are mixed to prepare a composite fermentation seed liquid. A culture medium composed of cassava residues and sawdust is used to prepare pretreated taiwanofungus castaneus culture medium. Then, the taiwanofungus castaneus strain is inoculated in the culture medium for fermentation, and a heat stress treatment step is introduced in the fermentation process. The method significantly promotes the synthesis and dissolution of active components of taiwanofungus castaneus triterpenoids and polysaccharides by the synergistic effect of specific strains and the combination of physical stress. The present application solves the technical problem of insufficient dissolution of active substances of taiwanofungus castaneus in the prior art by a unique strain combination and process design, and has high application value and market prospect.

This invention proposes a Bacillus compound inoculant, its preparation method, and its application, relating to the field of microbial technology. The method of this invention includes the following steps: selecting 18%-25% glucose, 18%-25% yeast extract, 0.3%-0.7% manganese sulfate, 1%-3% potassium dihydrogen phosphate, 1%-3% diammonium citrate, 3%-7% sodium acetate, and 0.1%-0.5% arginine by mass fraction, with the remainder being distilled water, and thoroughly mixing them to prepare a fermentation medium; selecting Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, and Bacillus lentigines, and obtaining a seed culture for inoculation at a constant temperature; mixing the various seed cultures in a ratio of 0.1-0.5:0.1-0.5:95-98:0.1-2:0.1-2 to prepare a compound inoculant, and fermenting to obtain the Bacillus compound inoculant. The compound microbial agent prepared by this invention can not only change the soil nutrient structure and the distribution of microbial communities in the soil, but also change the content of active ingredients in the rhizome of Ligusticum chuanxiong, thereby significantly promoting the growth of Ligusticum chuanxiong rhizome.

The invention relates to the technical field of soil improvement, and particularly discloses a compound microbial agent and application thereof. The compound microbial agent is prepared from the following viable bacteria: bacillus subtilis, bacillus licheniformis, bacillus circulans, bacillus mucilaginosus, nitrogen-fixing bacteria (Azobacter vinelandii), phosphate solubilizing bacteria (Acinetobacter sp.), potassium solubilizing bacteria (Sptotrophomonas cidaminiphila) and the like, and the compound microbial agent is prepared from the following active bacteria: bacillus subtilis, bacillus licheniformis, bacillus circulans, bacillus mucilaginosus, nitrogen-fixing bacteria (Azobacter vinelandii, phosphorus solubilizing bacteria (Acinetobacter sp.), potassium solubilizing bacteria (Sptotrophomonas cidaminiphila) and the like. The compound microbial agent provided by the invention can effectively improve soil ecology, promote crop growth and development, and improve crop yield and disease resistance.

The present application relates to a kind of parabacillus (Bacillus paralicheniformis) Bacillus paralicheniformis ) HMF14, the preservation number is CGMCC No.30480.The parabacillus HMF14 of the present application can resist salt and alkali, can simultaneously antagonize fusarium oxysporum, microdochium nivale var. avenae and xanthomonas campestris pv. tridii, also has toxic effect to pratylenchus penetrans and tetranychus cinnabarinus, can effectively prevent and treat wheat take-all disease and wheat black foot disease.

A biological control method for centipede breeding belongs to the field of special economic animal breeding technology. This invention uses a compound probiotic strain of *Trichoderma longicornis*, *Streptomyces flavus*, *Bacillus subtilis*, *Bacillus licheniformis*, *Clostridium butyricum*, and lactic acid bacteria, which is fully incorporated into the breeding substrate. After 24-72 hours of static setting, the bacteria fully colonize and form a stable antibacterial state. The bacterial community can remain dormant during periods of nutrient deficiency, low-temperature overwintering, or year-end static setting, and will revive upon contact with organic matter. The method is supplemented with springtails and pink woodlice, which preferentially consume harmful bacteria, clean up uneaten feed, and primarily feed on and decompose centipede feces, forming a mutually beneficial symbiotic relationship with the biocontrol bacteria. Their feces further nourish the bacterial community, extending the stable effective period of the bacterial community from 2 months to 4-6 months or more. Simultaneously, probiotics and vitamins are added to the feed to strengthen the centipede's constitution. This invention constructs a three-dimensional synergistic prevention and control system that integrates matrix inhibition of pathogenic bacteria, environmental residue removal, and internal administration for enhanced protection. This system enables drug-free ecological disease control, significantly reduces the incidence of diseases such as green muscardine, and improves the survival rate of centipede farming. It is suitable for large-scale centipede farming.

The invention relates to the technical field of microbial fermentation, in particular to a complex microbial inoculant as well as a preparation method and application thereof in promoting compost fermentation. The composite bacterial agent provided by the invention comprises a composite bacterial liquid and a matrix, wherein the composite bacterial liquid comprises bacillus licheniformis and bacillus coagulans; the mass ratio of the bacillus licheniformis to the bacillus coagulans is 1: (0.4-2.5). When the complex microbial inoculant provided by the invention is used for fermenting compost, the fermentation starting time in winter can be remarkably shortened, the core temperature of the compost is increased by 10-50 DEG C, and the fermentation period is shortened from conventional 12-16 days to 5-6 days.

The present document describes a composition comprising at least one lichen metabolite extract and at least one carrier comprising an ether, an ester, a terpene, an essential oil distillate, or a combination thereof, and, wherein the isolated lichen metabolite concentrate is solubilized in the carrier. The present document also describes an anti-microbial composition comprising at least one lichen metabolite extract and at least one carrier. The present document further describes methods of providing a lichen metabolite extract using said carriers.

The invention provides a microbial fertilizer capable of reducing the amount and increasing the efficiency of a chemical fertilizer and a preparation method thereof, and relates to the technical field of agricultural microbial fertilizers. The fertilizer is prepared from, by mass, 5%-10% of meat, 5%-8% of bones, 8%-12% of fruit leavening, 10%-15% of bean product leavening, 15%-20% of cottonseed meal, 3%-5% of various microbial agents, 8%-12% of corn flour, 8%-12% of bran and 15%-20% of deadwood rotten matter. The multiple microbial agents are composed of bacillus subtilis, bacillus licheniformis, phosphate-solubilizing and potassium-solubilizing bacteria and nitrogen-fixing bacteria. The preparation method comprises the steps of raw material pretreatment, mixed inoculation, high-temperature aerobic fermentation, post-curing and forming. Soil nutrients are activated through beneficial microorganisms, the application amount of a chemical fertilizer can be reduced by 20%-30%, the utilization rate of the chemical fertilizer is increased to 60% or above, meanwhile, the emergence rate of seedlings is increased to be larger than or equal to 95%, the pH value of saline-alkali soil is improved to be 0.5-1.0, pesticide residues are reduced to be larger than or equal to 40%, the sugar degree of agricultural products is increased to be 0.5-1.0 Brix, the cost is 15%-20% lower than that of existing bacterial fertilizer, and the fertilizer is suitable for planting of various crops.

The invention relates to a water regulating microbial preparation for breeding largemouth bass seedlings in a land-based round pond and application, and belongs to the technical field of aquaculture. The preparation aims to solve the problem of combined pollution caused by synchronous exceeding of ammonia nitrogen, nitrite and hydrogen sulfide in a water body due to accumulation of residual feed and excrement in a land-based round pool high-density breeding environment. According to the scheme, the microbial agent is prepared by physically mixing bacillus standard bacterial powder, photosynthetic bacteria standard bacterial powder, compound nitrifying bacteria standard bacterial powder and thiobacillus standard bacterial powder according to specific parts by weight, wherein the bacillus standard bacterial powder is prepared by mixing bacillus subtilis and bacillus licheniformis, and the thiobacillus standard bacterial powder is prepared by mixing bacillus subtilis and bacillus licheniformis. The composite nitrifying bacteria standard bacterial powder is formed by mixing nitrite bacteria and nitrifying bacteria, and the thiobacillus thiooxidans standard bacterial powder is formed by mixing thiobacillus thiooxidans and thiobacillus thioparus. The preparation is used for synchronously degrading ammonia nitrogen, nitrite and hydrogen sulfide in aquaculture water, so that normal development of gonads of largemouth bass parents is guaranteed, and the hatching rate of fertilized eggs and the survival rate of early-stage seedlings are increased.

The present application relates to the field of microorganism, and in particular to a method for producing vitamin B5 by using high-activity aspartate decarboxylase.The present application screens L-aspartate alpha-decarboxylase from Bacillus licheniformis, which has significantly higher activity than other sources of PanD in catalyzing the production of beta-alanine. The present application uses PanD from B. licheniformis to construct an engineered bacterium for fermentative production of vitamin B5, thereby eliminating the metabolic bottleneck of beta-alanine in biosynthesis of vitamin B5. Compared with the chemical method for producing vitamin B5 with high pollution, the present application has the advantages of renewable raw materials, easy treatment and resource utilization of waste residue, waste water and waste gas, etc., thereby being applicable to industrial production of vitamin B5 in practice and having important application value.

The application discloses a kind of microbial compound microbial inoculant and preparation method thereof, the microbial compound microbial inoculant includes microbial inoculum and its fermentation product and carrier;The microbial inoculum includes bacillus subtilis and bacillus licheniformis;The carrier is nitrogen-rich grafted biochar;The nitrogen-rich grafted biochar is prepared after grafting acrylic acid and modified monomer after being modified by nitrogen-rich biochar.The microbial compound microbial inoculant provided in the application can significantly improve the adsorption capacity and flocculation capacity of the microbial inoculant for particles in wastewater by compounding microbial inoculum with excellent flocculation capacity with charge-modified and adsorbed carrier to make microbial inoculant, thereby effectively reducing the content of COD components, ammonia nitrogen and total phosphorus in wastewater, and can be widely applied in the field of wastewater treatment.

The invention discloses preparation and application of bacillus licheniformis for decomposing pigeon feathers, and relates to the field of environmental resource application in the breeding industry, and the preparation method comprises the following steps: step 1, strain activation: firstly resuscitating a freeze-dried strain, and then culturing and activating by adopting a gradient activation mode; 2, preparing a seed solution, namely culturing the strain activated in the step 1 in a seed culture medium to obtain the seed solution; according to the method, the components of the pre-activation culture medium comprise 1% of corn flour, 0.5% of soybean meal and 0.1% of dipotassium phosphate, the production cost is low, and the preparation feasibility and economic benefits of the bacillus licheniformis are improved; the feather decomposition efficiency is improved by 2-3 times through substrate pretreatment, the residual feather amount is reduced, meanwhile, the fermentation period is shortened to 40-50 hours, and the feather decomposition efficiency of the pigeons is improved; in the fermentation tank, the activity of the keratinase can reach 600U / mL, so that the decomposition efficiency can be improved, subsequent reuse is facilitated, and the utilization rate is increased.