609 results about "Lichen" patented technology

The invention discloses a complex microbial inoculant for coupling straw decomposition and soil carbon sequestration and a preparation method thereof, the complex microbial inoculant can rapidly promote degradation of lignocellulose of straw in soil by compounding bacillus subtilis, bacillus licheniformis, pseudomonas aeruginosa and the like, provides a basic material energy source for activity of soil microorganisms, and has the advantages of simple preparation process and low cost. Meanwhile, corresponding microorganisms achieve long-term storage of organic carbon through humus stabilization and mineral substance combined carbon (MAOC) stabilization, the storage efficiency of the organic carbon is remarkably improved through nano Fe3O4, and under the synergistic effect of all strains in the complex microbial inoculant, the carbon loss of straw returning can be effectively reduced, the carbon immobilization capacity of soil is improved, and the soil quality is improved. The organic carbon content in the soil is increased.

The invention provides a southern red soil dry land deacidification soil conditioner and a preparation method thereof, and belongs to the technical field of soil conditioners. The preparation method comprises the following steps: embedding bacillus subtilis, bacillus licheniformis and bacillus amyloliquefaciens to prepare bacterial microspheres, adding the bacterial microspheres into gel formed by carboxymethyl chitosan and silk fibroin peptide to prepare sustained-release bacterial microsphere gel, uniformly mixing the sustained-release bacterial microsphere gel with porous silicon dioxide loaded with basalt and charcoal, and drying to prepare the acid-reducing soil conditioner for the southern red soil dry land. The acid-reducing soil conditioner for the southern red soil dry land not only improves the pH value of the soil, but also can loosen the soil and improve the air permeability of the soil to a certain extent, and promotes the absorption of crops to moisture and nutrient elements by improving the physical and chemical properties of the soil, so that the normal growth of the crops is ensured, and the acid-reducing soil conditioner has a wide application prospect.

The invention relates to a complex microbial inoculant for preventing and treating citrus huanglongbing as well as a preparation method and application of the complex microbial inoculant. The compound bacterial agent for preventing and treating the citrus huanglongbing is prepared from the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. The compound bacterial agent for preventing and treating the citrus huanglongbing comprises the following components in parts by weight: 5 to 10 parts of bacillus subtilis X1, 5 to 10 parts of bacillus pumilus X2, 5 to 10 parts of bacillus licheniformis X3, 5 to 10 parts of bacillus amyloliquefaciens X4 and 5 to 10 parts of pseudomonas fluorescens X5. After the complex microbial inoculant is added, the content of callose in infected leaves can be effectively reduced, the self-resistance mechanism of plants is induced, the disease resistance is improved, and the complex microbial inoculant has an obvious inhibiting effect and a growth promoting effect on Candidatus Liberibacter asiaticum.

The invention discloses a decomposition preparation containing bacillus licheniformis as well as a preparation method and application thereof, and belongs to the technical field of microorganisms. The decomposition preparation provided by the invention comprises bacillus licheniformis and Thermus arcuatus. The bacillus licheniformis is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 27, 2025, the preservation address is No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 35039. The bacillus licheniformis is separated from coastal saline-alkali soil, has high temperature resistance and salt resistance, and can secrete protease and lipase at the same time. After the bacillus licheniformis and the Thermus arcuatus are mixed, the synergistic effect is remarkable, the duck-derived red mud is effectively promoted to be converted into humic acid, and high-value resource utilization of the duck-derived red mud is achieved.

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

The invention relates to the technical field of agricultural wastes and microorganisms, in particular to a complex microbial inoculant for high-temperature composting and rapid decomposition of livestock and poultry manure and straw cellulose and a preparation method of the complex microbial inoculant. The compound microorganism bacterial liquid comprises streptomyces thermocarbonic oxide, bacillus stearothermophilus, bacillus subtilis, bacillus licheniformis, phanerochaete chrysosporium, trichoderma viride and aspergillus niger, and the bacterial liquid carrier is obtained by compounding modified charcoal and modified sepiolite through amidation reaction. According to the complex microbial inoculant provided by the invention, through the synergistic effect of the seven functional complementary microbial strains and the special three-dimensional reticulate carrier, rapid decomposition of high-temperature compost of livestock and poultry manure and straw cellulose is realized.

The invention belongs to the technical field of soil improvement, and relates to a soil conditioner for saline-alkali soil and a soil improvement method. The soil conditioner comprises vinegar residues, chitin, glucose, a composite biological preparation, a modified gel and a functional additive, the composite biological agent comprises bacillus subtilis powder, bacillus licheniformis powder, trichoderma spore powder, brown algae powder and light calcium carbonate; the functional auxiliary agent comprises modified bentonite, polyglutamic acid, pregelatinized starch and biochar potassium humate. Through the synergistic effect of multiple components and structural design optimization, systematic remediation of physical properties, chemical properties and biological activity of the saline-alkali soil is achieved. According to the technology, the limitation of single function of a traditional improvement measure is broken through, and the long-standing technical problems of return salt rebound, low fertility, microbial community imbalance and the like in saline-alkali soil treatment are effectively solved by constructing a multi-layer and multi-dimensional soil improvement system.

According to the method, bacillus licheniformis bacterial liquid and calcium ion liquid are sequentially, slowly and evenly sprayed on the surface of a soil body, soil particles on the surface of a slope are cemented, a layer of calcium carbonate protection film is formed on the surface of the soil body, the anti-scouring capacity of the soil body can be effectively improved, flowing of the soil particles is reduced, and plant growth is protected; the adopted bacillus licheniformis can also increase the number of effective microbial communities in soil, improve the root system environment, promote plant growth, inhibit reproduction of harmful microorganisms, improve the granular structure of the soil, improve the soil, regulate the pH value of the soil, improve the water storage, energy storage and heat preservation of the soil, improve the disease resistance of crops and promote the maturity of plants; after vegetation grows, the effect of water and soil conservation can be improved, generated calcium carbonate can also bond soil particles, water and soil loss is reduced, bacillus licheniformis plays a dual role, multiple utilization of resources is achieved, and wide application prospects are achieved.

The invention discloses an organic fertilizer for planting local cereal crops in a plateau region and a preparation method thereof, and belongs to the technical field of agricultural planting. The organic fertilizer for planting local cereal crops in the plateau region is prepared from the following preparation raw materials: decomposed animal waste, vermiculite, humic acid, citric acid, fish meal, bone meal, straw, cake meal and a compound microbial agent, the carrier is prepared from decomposed animal waste, vermiculite, humic acid and citric acid, so that the mechanical strength and durability of the carrier can be improved, and a good physical state can be kept in the storage, transportation and use processes; through compound use of bacillus licheniformis, bacillus subtilis and bacillus laterosporus and adjustment of the dosage ratio of all strains and the viable count of the compound microbial agent, the soil micro-ecological environment can be improved, breeding of beneficial microorganisms in soil is promoted, growth of harmful bacteria is inhibited, and the soil fertility and the water and fertilizer retention capacity are improved; the fertilizer has a good promotion effect on the growth of spring wheat, oat and other plateau cereal crops.

The invention relates to a decomposition-promoting phosphorus-dissolving deodorizing microbial agent for composting and a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, paenibacillus zerzeri C-A and bacillus chestnut brown SC in a mass ratio of 1.5: (1-3): (1.5-4). The viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 1 * 10 < 9 > CFU / g. The preparation method comprises the following steps: (1) strain activation; (2) strain amplification culture; (3) bacterial powder preparation; and (4) strain compounding. The invention relates to application in clean composting of organic wastes. The invention has the following advantages: the three strains in the microbial inoculum do not generate antagonism, and can obviously accelerate the conversion of insoluble phosphorus, promote decomposition and improve the effect of removing NH3 and H2S.

The invention relates to the technical field of soil improvement, and discloses a soil improvement repairing agent and a preparation method thereof. The preparation method comprises the following steps: adding biochar, modified eugenol, modified benzothiazolyl pyridine, bacillus licheniformis and diatomite into a mixer, mixing and stirring, extruding and granulating in a granulator, and drying to obtain the soil improvement repairing agent. Eugenol can destroy a membrane structure and denature protein, so that a bacteriostatic effect is achieved, and a healthy soil microenvironment is provided for crops; the quaternary ammonium salt can destroy the membrane structure of pathogenic microorganisms, plays a role in killing the microorganisms, can improve the antibacterial effect of the remediation agent, and prevents and treats spread diseases of soil; nitrogen atoms and sulfur atoms in benzothiazolyl pyridine molecules can coordinate with free heavy metal ions in the soil, the heavy metal adsorption effect of the remediation agent is improved, the production value of the soil is gradually restored, meanwhile, boric acid capable of being absorbed by plants can be released through hydrolysis of pinacol ester, and the fertility of the soil is improved.

The invention discloses a coated slow-release solid water-soluble fertilizer as well as a preparation method and application thereof. The coated slow-release solid water-soluble fertilizer comprises the following components in parts by weight: 15-20 parts of a nitrogen source, 8-12 parts of a phosphorus source, 15-25 parts of a potassium source, 3-8 parts of humic acid and 10-15 parts of charcoal powder, the composite material comprises the following components in parts by weight: 0.5-1.5 parts of functional microorganisms, 1-2 parts of microbial metabolite, 2-5 parts of seaweed extract and 5-8 parts of chitosan-gelatin composite material. The functional microorganisms are bacillus subtilis, bacillus licheniformis and azospirillum which are adsorbed on the charcoal powder. The coated slow-release solid water-soluble fertilizer provided by the invention has the effects of supplying nutrients and preventing and treating soil-borne diseases, achieves multiple effects by one fertilizer, and can improve the crop nutrient utilization rate, inhibit the soil-borne diseases and promote the diversity of soil microorganisms.

The invention discloses a compound micro-ecological preparation for aquatic products as well as a preparation method and application thereof, and belongs to the technical field of micro-ecological preparations. The microecological preparation disclosed by the invention comprises clostridium butyricum, bacillus licheniformis, enterococcus faecalis and lactobacillus plantarum. The microecological preparation is combined with concentrated cottonseed protein for use, can replace fish meal, is added into feed, can remarkably improve the immunocompetence, oxidation resistance and digestive enzyme activity of animals, and can improve the relative abundance of beneficial bacteria in intestinal tracts, inhibit the growth of pathogenic bacteria and regulate the microecological balance of the intestinal tracts after entering the intestinal tracts of the animals. According to the invention, aerobic bacteria and anaerobic bacteria are cooperatively used, oxygen in the surrounding environment is greatly consumed by bacillus licheniformis and enterococcus faecalis, the proliferation of beneficial bacteria such as clostridium butyricum and lactobacillus plantarum is promoted, and the probiotic effect is fully played.

The invention provides an environment-friendly microbial strain and a water treatment agent preparation process, and relates to the field of water treatment. The environment-friendly microbial strain is prepared from the following raw materials in parts by weight: 20 parts of nitrifying bacteria, 20 parts of denitrifying bacteria, 35 parts of bacillus and 25 parts of EM bacteria, the nitrifying bacteria comprise nitrosomas and nitrobacteria, and the denitrifying bacteria comprise nitrosomas and nitrobacteria. The denitrifying bacteria comprise denitrifying bacilli and sternobacter, the bacillus comprises bacillus subtilis and bacillus licheniformis, and the EM bacteria comprise rhodopseudomonas, lactococcus lactis and saccharomyces cerevisiae. Through flora screening and culture, the culture efficiency is improved, through a fermentation process, fermentation liquor overflow and insufficient dissolved oxygen caused by excessive foam are effectively prevented, the stability of the fermentation process is ensured, through preparation preparation, a good packaging material and a sealing technology, moisture absorption and pollution of a microbial inoculum are prevented, and the quality and safety of a product are ensured.

The invention discloses an extreme thermophilic charcoal complex microbial inoculant and application thereof in aerobic composting of pig manure, and belongs to the technical field of microorganisms. The invention firstly discloses a strain of thermophilic thermophilic bacteria N1209, and secondly discloses an extreme thermophilic biochar complex microbial inoculant, which comprises geobacillus thermoglucosidase BGSC 95A1, bacillus licheniformis ZR-1, the thermophilic thermophilic thermophilic bacteria N1209 and geobacillus stearothermophilus L5. The extremely thermophilic biochar complex microbial inoculant has the characteristic of high temperature resistance, compared with a single microbial inoculant, produced amylase, protease and lipase are higher in activity and show synergistic advantages, decomposition of pig manure can be remarkably promoted, the extremely thermophilic biochar complex microbial inoculant has advantages in the aspects of nitrogen element retention and CH4 and N2O emission reduction in the pig manure composting process, and the extremely thermophilic biochar complex microbial inoculant can be applied to the field of pig manure composting. And a new method is provided for treatment of livestock breeding wastes.

The invention belongs to the technical field of coastal saline-alkali soil remediation, and particularly relates to a composite microbial agent for coastal saline-alkali soil remediation. The microbial agent is in a granule form, and comprises a carrier, which is a mixture of straw powder and duck manure; the active flora comprises bacillus licheniformis, bacillus megaterium, brevibacillus laterosporus, trichoderma harzianum spore powder, paenibacillus polymyxa, halomonas and pseudomonas fluorescens, the active flora is adsorbed in the carrier, and the halomonas and the pseudomonas fluorescens are subjected to embedding treatment; and the spraying film is sprayed on the surface of the carrier. Through the synergistic effect of multiple strains, the improvement effect on saline-alkali soil and the growth promoting effect on crop growth are improved, and the defect that the function of a single strain is limited is overcome.

The invention belongs to the technical field of bioengineering, and particularly relates to an activity-improved tyrosinase mutant obtained by site-directed mutagenesis through an overlapping PCR technology, and preparation and application thereof. The tyrosinase mutant is obtained by mutation containing F337I / F352I on the basis of wild type tyrosinase as shown in SEQ ID NO.1, the enzyme activity of the tyrosinase mutant is obviously improved compared with that of the wild type tyrosinase, the F337I / F352I mutant gene is used for constructing a recombinant vector, and the recombinant vector is successfully expressed in bacillus subtilis WB600, bacillus amyloliquefaciens CGMCC No.11218 and bacillus licheniformis 2709, so that the tyrosinase mutant is obtained. The recombinant strain with improved enzyme production activity is further optimized through a fermentation process to obtain the novel tyrosinase.

The invention discloses a novel microbial sludge reduction inoculant and a preparation method and application thereof, and belongs to the technical field of microorganisms, the microbial sludge reduction inoculant comprises a composite inoculant and a biomass additive; the complex microbial inoculant is prepared from the following components in parts by weight: 10 to 12 parts of lactic acid bacteria, 18 to 22 parts of saccharomycetes, 6 to 8 parts of bacillus licheniformis, 12 to 16 parts of bacillus subtilis, 4 to 6 parts of bacillus amyloliquefaciens, 7 to 9 parts of methane sarcina and 3 to 5 parts of horsehair methane bacteria; the biomass additive is fruit peel waste. According to the method disclosed by the invention, a biodegradation system with high activity of various required enzymes can be formed by utilizing the mutual action of microbial flora, dead or aged thalli in the activated sludge can be effectively destroyed and decomposed, meanwhile, organic matters can be decomposed, the sludge is subjected to reduction treatment under the condition that the effluent quality is not influenced, the treatment cost is low, and the treatment effect is good. The method is green and pollution-free and worthy of popularization.

The invention discloses a microbial compound feed for improving disease resistance of blue crabs and a preparation method of the microbial compound feed, and belongs to the technical field of feed preparation. The microbial compound feed comprises the following raw materials in parts by weight: 30-40 parts of fermented soybean meal, 20-25 parts of bran, 15-20 parts of fish meal, 5-8 parts of a compound microbial agent, 3-5 parts of algal polysaccharides, 2-3 parts of a radix astragali extract, 1-2 parts of chitosan, 2-3 parts of a mineral premix and 1-1.5 parts of compound vitamins, wherein the compound microbial agent comprises bacillus licheniformis, lactobacillus acidophilus and saccharomyces cerevisiae. The microbial compound feed disclosed by the invention comprehensively improves the disease resistance of the blue crabs and improves the survival rate through various ways, such as nutrition balance, immune regulation and intestinal health improvement, so that the breeding cost is reduced, and the income of farmers is increased.

The present invention belongs to the technical field of microbial fermentation, and discloses the application of a compound bacterium agent of Lactobacillus rhamnosus and Bacillus licheniformis for reducing acids and higher alcohols, Lactobacillus rhamnosus, and in Baijiu. Lactobacillus rhamnosus L21 has a preservation number of: CGMCC NO. 32609, a preservation date of: November 13, 2024, and a preservation unit of: China General Microbiological Culture Collection Center. The use of the compound bacterium agent of Lactobacillus rhamnosus L21 and Bacillus licheniformis XDNZ_Bl_27 can effectively reduce organic acids such as lactic acid and propionic acid in the Baijiu fermentation system, and at the same time can also reduce the content of n-propanol in the fermented grains, which is beneficial to the regulation of acidity and higher alcohols in the process of Baijiu production.

The invention discloses a composition containing cactus fermentation liquor and a preparation method and application of the composition in cosmetics, the cosmetic composition containing the cactus fermentation liquor comprises the cactus fermentation liquor, Ectoine and sodium hyaluronate according to a specific proportion, the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on succulent stems of cactus, and the cactus fermentation liquor is prepared by performing enzymolysis pretreatment on the succulent stems of the cactus. Sequentially inoculating bacillus licheniformis and lactobacillus plantarum for sequential fermentation; and finally, purifying and enriching by ultrafiltration and nanofiltration two-stage membrane separation technologies. A product rich in small molecule active peptide and specific organic acid is obtained through biotransformation process combination, so that the final cosmetic composition has remarkable skin barrier repairing and anti-oxidation effects.

The invention discloses a planting method for increasing soil organic matters and carbon sequestration, belongs to the technical field of ecological soil improvement, and provides a planting method for increasing soil organic matters and carbon sequestration, which comprises the following steps: applying a complex microbial inoculant on the surface of a planting land; planting an arbor, and then planting caragana twisting in planting gaps of the arbor; the composite microbial inoculant is prepared from bacillus cereus (bacillus cereus) and bacillus parlicheniformis (bacillus parlicheniformis), and the composite microbial inoculant is prepared from bacillus cereus (bacillus cereus) and bacillus parlicheniformis (bacillus parlicheniformis); the arbor is picea crassifolia, pinus sylvestris and pinus tabulaeformis. According to the method, picea crassifolia, pinus sylvestris and pinus tabulaeformis of different forest ages are planted in a mixed mode, and caragana twistable is matched, so that the effects of maximizing soil organic matter accumulation and enhancing carbon sequestration in the Gansu Xieu region are achieved.

The invention discloses a probiotic animal feed and a preparation method thereof, and belongs to the technical field of animal feeds, the probiotic animal feed comprises the following components by mass: 40-55 parts of caragana microphylla powder, 20-30 parts of fermented soybean meal, 3-5 parts of a composite probiotic preparation, 5-10 parts of a medicinal and edible additive, and 2-3 parts of a mineral premix. According to the composite probiotic preparation, bacillus licheniformis, pediococcus pentosaceus and saccharomyces cerevisiae boulardii synergistically improve the fiber decomposition efficiency, inhibit pathogenic bacteria and reduce ammonia nitrogen emission. The medicinal and edible additive is of a multi-layer micro-capsule structure, the outer layer is carboxymethyl chitosan and copper gluconate modified nano zeolite, active substances in the middle layer are rapidly released through rumen pH response, and the middle layer contains a yucca extract, a liquorice extract and other components and can inhibit urease activity and ammonia-producing bacteria; in the inner layer, a nano-selenium-vitamin E compound, curcumin and the like are slowly released through sodium alginate-pectin gel, so that intestinal metabolism is optimized. The process of pretreating caragana microphylla through steam explosion and performing solid-state fermentation on soybean meal through aspergillus oryzae is utilized, so that grain-free production is realized.

The invention relates to the technical field of pulping, in particular to a bamboo reed raw material biological fermentation cellulose pulping method and application thereof. Bacillus licheniformis, bacillus subtilis, bacillus pumilus and bacillus cereus are compounded into a complex microbial inoculant, the fermented bamboo reed raw material is used for pulping, all strains are mutualistic and synergistic, meanwhile, a rich complex enzyme system is provided, the prepared paper is good in physical performance and high in quality, pulping is conducted through biological treatment, and the paper is environmentally friendly and environmentally friendly. The use of a large amount of chemical agents in the traditional pulping is avoided, and the discharge of wastewater in the traditional pulping process is reduced.

The invention provides a 2-succinyl-5-enol acetonyl-6-hydroxy-3-cyclohexene-1-carboxylic acid (SEPHCHC) synthase mutant, which is characterized in that on the basis of a bacillus subtilis SEPHCHC synthase gene MenD, 12 SEPHCHC synthase mutants are obtained by virtue of a site-specific mutagenesis PCR (Polymerase Chain Reaction) technology, and the SEPHCHC synthase gene MenD is used as a base. Recombinant bacteria of bacillus subtilis or bacillus natto or bacillus licheniformis or bacillus amyloliquefaciens for expressing the mutant are respectively constructed, and the yield of heptaene menadione fermented by the recombinant bacteria is 74%-203% of the yield of heptaene menadione fermented by the recombinant bacteria of bacillus subtilis for expressing wild type SEPHCHC synthase MenD.

The invention relates to the technical field of fertilizers, in particular to a fulvic acid type compound microbial organic fertilizer and a preparation method thereof. The method comprises the following steps: preparing compound microorganism bacterium powder from bacillus subtilis, bacillus megatherium, bacillus licheniformis, pseudomonas fluorescens and bacillus psychrosaccharolyticus; preparing a solid-phase substance from ferric chloride hexahydrate, terephthalic acid and dimethylformamide; generating hydrogel on the surface of the chemical fertilizer in situ, and adding a solid-phase substance to obtain a slow-release chemical fertilizer; and preparing an organic fertilizer, and mixing the organic fertilizer with the compound microbial powder, the slow-release chemical fertilizer and the organic fertilizer to obtain the compound microbial organic fertilizer. According to the compound microbial organic fertilizer provided by the invention, the chemical fertilizer, the organic fertilizer and the microbial fertilizer synergistically promote the growth of plants, and the use of the chemical fertilizer is reduced.

The invention belongs to the technical field of agricultural microbial control, and particularly relates to a growth-promoting and disease-preventing compound microbial agent and a preparation method and application thereof, and the compound microbial agent specifically comprises microbial powder, a growth auxiliary material and a drying agent; the microorganisms comprise vermiform yellow, trichoderma harzianum, bacillus velezensis, bacillus licheniformis and brevibacillus laterosporus. According to the compound microbial agent, through the synergistic effect of multiple strains, the control effect of the compound microbial agent on verticillium dahliae is enhanced, and the defects that a single biocontrol bacterium is single in biocontrol mechanism, poor in environment adaptability, incapable of being colonized in soil for a long time and unstable in control effect are overcome; the compound microbial agent can increase the yield of cotton and pepper and improve the quality of cotton fibers, and provides scientific and technological support and decision service for realizing green, environment-friendly, cost-saving and efficiency-increasing modern agriculture.

The invention discloses a method for improving uranium immobilization by bacillus by using an extracellular polymeric substance, and belongs to the field of environmental engineering, the bacillus licheniformis X-113 is separated from a uranium-polluted environment and is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 20251207, and the bacillus licheniformis X-113 is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M 20251207. By optimizing the EPS culture conditions, the fixing capacity of the EPS-Bacillus sp.X-113 on uranium is remarkably enhanced compared with that of Without EPS-Bacillus sp.X-113, and the fixing capacity of the EPS-Bacillus sp.X-113 on uranium is remarkably enhanced compared with that of Without EPS-Bacillus sp.X-113. And numerous functional groups in EPS, such as carboxyl, hydroxyl, amino and the like, can be subjected to complexation, ion exchange and other reactions with uranium ions to fix the uranium ions, so that the uranium fixation of the bacillus is greatly improved.

The invention provides compost and a preparation method thereof, and relates to the technical field of organic fertilizers, the compost comprises the following components by weight: 900-1100 parts of crop straw, 200-300 parts of animal excreta, 100-200 parts of an organic auxiliary material, and 1.5-7.5 parts of a complex microbial inoculant; the complex microbial inoculants comprise lactic acid bacteria, bacillus licheniformis, thermophilic trichoderma, actinomycetes and white rot fungi. The preparation method of the compost mainly comprises the following steps: stacking the crop straws and the organic auxiliary materials layer by layer, and compacting; uniformly sprinkling animal excrement on each layer, and then inoculating a complex microbial inoculant; punching a hole in the top of the pile to monitor the moisture content in the pile in the composting process; pile turning treatment is carried out in the composting process. According to the invention, the complex microbial inoculants are adopted for compost maturing treatment, so that the maturing speed can be increased, the maturing effect can be improved, and the content of nutrient substances in compost is increased.

The invention relates to the technical field of bio-fertilizers, and particularly discloses a bio-organic fertilizer for preventing and treating root rot of codonopsis pilosula as well as a preparation method and application of the bio-organic fertilizer. The bio-organic fertilizer for preventing and treating root rot of codonopsis pilosula provided by the invention comprises a kitchen fermentation base material and a complex microbial inoculant in a weight ratio of 100: (1-10), the complex microbial inoculant comprises one or more of bacillus atrophaeus, bacillus coagulans, bacillus licheniformis, bacillus velezensis and brevibacillus laterosporus; the invention further provides a preparation method of the bio-organic fertilizer and application of the bio-organic fertilizer in prevention and treatment of root rot of codonopsis pilosula. The bio-organic fertilizer provided by the invention can degrade glyphosate in soil on one hand, and also can reduce the occurrence of root rot of codonopsis pilosula on the other hand, so that the growth of codonopsis pilosula and the accumulation of active value components of codonopsis pilosula can be promoted.