48 results about "Total Viable Count" patented technology

The invention relates to a liquid compound biological fertilizer with long shelf life as well as a preparation method and application thereof. The liquid compound biological bacterial fertilizer comprises the following components in parts by weight: 2-5 parts of microbial thalli, 80-130 parts of compound nutrients, 10-30 parts of thalli protective agents and 0.5-3.0 parts of additives, wherein the microbial thalli is metered in terms of the wet weight of deposition obtained after fermentation liquor is centrifugalized, the microbial thalli include bacillus thuringiensis, bacillus megaterium, bacillus mucilaginosus and bacillus amyloliquefaciens, and the total viable count achieves 5*10<8>-5*10<9>/mL. The liquid compound biological bacterial fertilizer disclosed by the invention can be used as a biological pesticide, can be directly applied to the spraying of plant surfaces or applied to seed soaking, root irrigation, and the like, has the effects of insect resistance, phosphate solubilization, potassium solubilization, organic matter solubilization, pest resistance, and the like and is long in shelf life.

The invention applies to provide a microbial deodorant which is a microbial colony composed of lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and rhodopseudomonas palustris, wherein counted by 108CFU (colony-forming unit)/ml as a unit, the total viable count is not less than 10, the lactobacillus plantarum count is not less than 3, the saccharomyces cerevisiae count is not less than 3, the rhodopseudomonas palustris count is not less than 2 and the bacillus subtilis count is not less than 2. The microbial deodorant applied by the invention has stronger zymolysis ability under both aerobic and anaerobic conditions, and the beneficial bacteria mutually play a role in inhibiting the growth of putrefactive bacteria and interrupting the putrefactive process, secrete organic acid while reproducing quickly, and generate antioxidation substances, thus converting odorous substances such as NH3, H2S, indoles and the like into non-odorous substances.

The invention discloses a microbial preparation for gangue reclaimed soil. The microbial preparation comprises three microbial preparations which are respectively used independently, wherein the I type microbial preparation contains bacillus subtilis and bacillus licheniformis; the II type microbial preparation contains bacillus mucilaginosus and the bacillus licheniformis; the III type microbial preparation contains azotobacter chroococcum, bacillus megaterium, the bacillus mucilaginosus and lysine bacillus; the total viable count of every gram of the microbial preparation is (2-200)*10<8> CFU. The gangue reclaimed soil is covered in layers by using the microbial preparation disclosed by the invention, a first gangue layer, a sticky loess and fly ash mixing covering layer, a second gangue layer, a fly ash covering layer, a fine granular gangue layer and a loess covering layer are sequentially arranged from bottom to top, the I type microbial preparation is applied to the sticky loess and fly ash mixing covering layer, the II type microbial preparation is applied to the fly ash covering layer, the III type microbial preparation is applied to the loess covering layer, and crops are planted on the loess covering layer.

The invention discloses a salt-resisting compound bacterium agent for disposing kitchen waste. The compound bacterium agent is composed of a compound thallus and a carrier auxiliary material, wherein the compound thallus comprises a basic treatment thallus; the basic treatment thallus is composed of bacillus subtilis, bacillus coagulans and saccharomyces cerevisiae; bacillus subtilis in the basic treatment thallus accounts for 30%-65% of a total viable count; bacillus coagulans thallus accounts for 20%-65% of the total viable count; and saccharomyces cerevisiae accounts for 5%-50% of the total viable count. The compound bacterium agent disclosed by the invention is suitable for a kitchen waste disposer with heat-insulation, air-exchanging and agitating functions; and a stable micro-ecology is formed by microorganisms in the bacterium agent and the compound bacterium agent can resist high salt, so that residues obtained by disposing the kitchen waste can be used as the bacterium agent to continually dispose the kitchen waste for the next time, and the compound bacterium agent can be used for a long period when being added at a time.

The invention belongs to the field of microbial technology and specifically relates to a composite microbial agent for treating high-ammonia-nitrogen sewage and a preparation method and application thereof. The composite microbial agent contains nitrobacteria, nitrite bacteria and bacillus subtilis. The viable count of nitrobacteria is 1.0*108-2*108 cfu/mL; the viable count of nitrite bacteria is 1.0*108-1.5*108 cfu/mL; and the viable count of bacillus subtilis is 1.0*108-2.0*108 cfu/mL. The total viable count is 3.0*108-5.5*108 cfu/mL. The composite microbial agent is used for treating high-ammonia-nitrogen sewage, is simple to use, is low-cost and has significant effects. The highest removal rate of ammonia nitrogen in sewage reaches 98%, COD removal rate reaches 85%, BOD removal rate reaches 92%, removal rate of total sulfur reaches 90%, and removal rate of sewage chroma reaches 32%.

The invention discloses mixed probiotic tablets and a low-temperature coating preparation method thereof and belongs to the field of probiotic tablets. The mixed probiotic tablets are prepared from 2-7 parts of a lactobacillus acidophilus KT-A1 agent, 2-7 parts of a lactobacillus casei Zhang agent, 2-7 parts of a bifidobacterium animalis subsp. lactis V9 agent and 2-7 parts of a lactobacillus plantarum P-8 agent with a low-temperature coating technology. According to the probiotic tablets prepared with the method, bacterium loss of probiotics in a tablet preparation process is avoided, after the tablets are stored for 5 months at the temperature of 37 DEG C, the total viable count of probiotics in the product keeps not lower than 5*10<9> CFU/g; furthermore, storage stability of the probiotics in the shelf life of the product is greatly improved, weight of each tablet is 0.7-0.9 g, the hardness reaches 7 N/cm<2> or higher, the surface is smooth, and the phenomenon of powder falling is avoided. Besides, the mixed probiotic tablets have excellent resistance to gastrointestinal digestive juice, improve capacity of intestinal microbial community structure of a human body and have a regulating function for body immunity.

The invention discloses a compound microbial agent which is prepared from nitrifying bacteria, nitrosomonas, single cell bacteria denitrification salt, salt denitrifying bacterium vibrio cholera, achromobacter denitrificans, bacillus thuringiensis and bacillus subtillis, wherein the total viable count is more than 5.9x10<10>cfu/g; the nitrifying bacteria and the nitrosomonas account for 10 to 20 percent of the total viable count; the single cell bacteria denitrification salt, the salt denitrifying bacterium vibrio cholera and achromobacter denitrificans account for 15 to 20 percent of the total viable count; the bacillus thuringiensis and the bacillus subtillis account for 5 to 10 percent of the total viable count. The compound microbial agent has the benefits that the compound microbial agent is fed at a time, and no bacteria is required to be kept added after the system is stabilized; therefore, the operation cost of sewage treatment is reduced, and the convenience of sewage treatment operation is enhanced.

The invention relates to the field of microbial preparations and particularly discloses a compound microorganism bacterium agent and a preparation method and application thereof. The compound microorganism bacterium agent comprises lactic acid enterococcus faecalis, bacillus subtilis and bacillus megaterium, wherein the total viable count of the agent is not less than 3.5*108 cfu/mL. The compound microorganism bacterium agent is composed of reasonable strains according to composition characteristics of kitchen filtrate, can quickly degrade harmful substances in the kitchen filtrate, has a remarkable effect on degrading COD, BOD, ammonia nitrogen and the like in the kitchen filtrate and can effectively degrade organic matter to greatly relieve odor of the kitchen filtrate; the processed kitchen filtrate can also be used as a raw material of compound microorganism water-soluble fertilizer, so that the utilization rate of energy sources is increased, and environmental pollution caused by direct discharge of the kitchen filtrate is reduced.

The invention discloses a formula of a probiotic soft drink base on two strains of lactic acid bacteria and a processing technology thereof. The invention is characterized in that three-stage static submerged liquid fermentation is adopted, the fermentation time of each stage is 48 hours, the fermentation temperature is 40+/-2 DEG C, and bacterial liquid obtained by the final-stage fermentation can be packaged into a product after precipitation and filtering; a culture medium for fermentation trial seeds is a common MSR culture medium; and a culture medium for expanding fermentation and production liquid fermentation is as follows: 2 percent of soybean milk, 1.2 percent of sucrose, 3 per thousand of Rumfor lactic acid bacteria culture medium synergist (containing sodium acetate, ammonium citrate, magnesium sulfate and the like). The processing technology has simple and convenient operation, and the processed product is colorless clear transparent liquid with slight fermentation odor; the pH of the product is 2.5-4.5, and the quality guarantee period thereof is not less than two months; and the total viable count cfu of the two strains is not lower than 310<7>/ml.

The invention provides a healthcare animal growth promoter containing soybean oligosaccharides and clostridium butyricum. The healthcare growth promoter is characterized by being prepared by taking the soybean oligosaccharides and the clostridium butyricum as main active ingredients and attapulgite as a carrier and compounding, wherein the soybean oligosaccharides account for 15%-40% of the total weight, the clostridium butyricum accounts for 30%-55% of the total weight, and the attapulgite accounts for 5%-55% of the total weight; the purity of the soybean oligosaccharides is equal to or higher than 95%; the total viable count of the clostridium butyricum is equal to or higher than 1.0*1010 CFU/g; the additive amount of the healthcare growth promoter in a feed is 0.05%-2%. The healthcare growth promoter has the benefits as follows: (1) the growth speed of domestic animals can be remarkably increased; (2) the immunity and the breeding safety of the domestic animals can be improved and the survival rate of the domestic animals can be increased; (3) the raw materials are safe, stable, nontoxic, pollution-free and residue-free, and the novel healthcare animal growth promoter is safe, efficient and green.

The invention discloses special biological type organic-inorganic compound fertilizer for peanuts. The special biological type organic-inorganic compound fertilizer is prepared from original fertilizer, an organic material and a microbial agent, wherein the original fertilizer contains 30%-40% by mass of N+P2O5+K2O, N content is 12%-15% by mass, P2O5 content is 8%-12% by mass, K2O content is 10%-13% by mass, organic matter content is higher than or equal to 15% in parts by mass, moisture (fresh sample) content is lower than or equal to 30% in parts by mass, total viable count is larger than orequal to 0.02 billion/g, urea formaldehyde content is 3%, active humic acid content is 5%-10%, and the total amount of amino acid is 0.4%-3%. With addition of a certain amount of urea formaldehyde, fertilizer particle strength is effectively improved, and fertilizer quality is improved; with addition of a certain quantity of humic acid, amino acid and the microbial agent, nutrients required for peanut lifetime are effectively guaranteed, fertilizer efficiency and crop yield are increased, quality is improved, and the compound fertilizer has special effects that land utilization and land maintenance are combined, long acting and quick acting are synergistic, water retention and fertilizer conservation effects are obvious and the like; compared with only application of nitrogen, phosphorusand potassium fertilizer, absorption and utilization rate of nutrients can be increased by about 30%-50%, peanut yield can be remarkably increased, and peanut quality can be remarkably improved.

The invention discloses a pretreatment method for enhancing anaerobic sludge digestion by virtue of a compound microorganism bacterium agent. The pretreatment method comprises the following steps: concentrating residual sludge, adding a compound microorganism bacterium agent, performing stirring reaction, inoculating the pretreated residual sludge into an anaerobic digestion pond according to a certain ratio, and performing mesophilic anaerobic digestion on anaerobic sludge, wherein the compound microorganism bacterium agent is prepared by mixed fermentation of 5%-20% of each of bacillus subtilis, streptomyces albus, streptomyces griseus, candida utilis, aspergillus sojae, lactobacillus bulgaricus, lactococcus lactis, rhodopseudomonas palustris, rhodopseudomonas capsulate and rhodopseudomonas spheroides, the total viable count is not less than 2*108/ml, and the pH value of the compound microorganism bacterium agent is 3-4. According to the method, the sludge hydrolysis process can be accelerated, the sludge retention time can be shortened, the biological gas production rate can be increased, and the anaerobic digestion efficiency can be improved.

The invention discloses compound probiotic fermented feed for sow breeding and a preparation method of the feed. The compound probiotic fermented feed for sow breeding is prepared from the components in parts by weight as follows: 13-20 parts of hawthorn fruit residues, 10-18 parts of soybean molasses, 25-35 parts of corn germ meal, 5-10 parts of corn flour, 3-11 parts of a compound probiotic solution and 30-38 parts of water. The invention further provides the preparation method of the compound probiotic fermented feed for sow breeding. The compound probiotic fermented feed has the total viable count larger than 2.37*10<8> cfu/g, has a light tawny color, has a slightly acid smell and can effectively increase the feed intake of sows, enhance the reproductive capacity of the sows, improve milk quality, increase the annual live piglet supply number, reduce oxidative stress and effectively improve the pig breeding efficiency.

The invention discloses a yoghurt starter with endogenous flavoring and a preparation method and application thereof. In the yoghurt starter with endogenous flavoring, a mass ratio of Lactobacillus delbrueckii subsp. bulgaricus SITCC No. 10008 to Streptococcus thermophilus SITCC No. 10010 is 1: 1-3 and the total viable count is at least 1*10<9> cfu/mL. In production of solidified yogurt, the curdtime is short and the curd state is good. Compared with the commercially available yogurt, the yoghurt provided by the invention has good aroma, has the highest butanedione content of 23.47+/-0.20 mg/L and can satisfy the aroma requirements on the solidified yogurt without other strains and food flavors.

The invention belongs to the field of biological prevention and treatment, and provides antibiological inoculant for preventing and treating cucumber root knot nematode disease. The composite microbial inoculant is prepared from glomus mosseae inoculums and bacillus subtilis fermentation broth; the composite inoculants is selected from nine simple inoculants and twelve composite inoculants according to the development condition and nematode resistance condition; each composite microbial inoculant includes 5000 inoculum potential glomus mosseae; the total viable counts of bacillus subtilis fermentation broth reach 3*10<8>CFU/ml; glomus mosseae and bacillus subtilis inoculants are compounded and show obvious a synergistic interaction effect on preventing and treating root knot nematode by being compared with individual glomus mosseae inoculants or bacillus subtilis inoculants, and the preventing effect of root knot nematode is also obviously improved.

The invention relates to the field of feed additives and discloses a composite micro-ecological preparation and application thereof. The composite micro-ecological preparation disclosed by the invention comprises probiotics, organic acids, vitamins and oligosaccharides, wherein the total viable count of the probiotics is more than or equal to 1*10<9> cfu/g; the organic acids account for 10-30% of the total weight; the oligosaccharides account for 1-10% of the total weight; and the vitamins account for 5-30% of the total weight. Tests indicate that the composite micro-ecological preparation disclosed by the invention has the effects of improving the growth rate of body mass, improving the anti-stress capability, improving the feed utilization rate and reducing aquaculture death rate. The composite micro-ecological preparation disclosed by the invention can be prepared into veterinary drugs, feed additives or nutritious supplementary to be singly used, also can be mixed in aquatic animal feed to be directly fed, is wide in application range and has excellent economic and social effects.

The invention discloses a method for evaluating rotting capability of microbes in an aquatic product. According to the method, the aquatic product inoculated with specific microbes is stored, the volatile base nitrogen (TVB-N) value and the total viable count (TVC) in the storage process are periodically detected by a chemical method, and obtained numerical values are substituted into a formula for calculating the rotting capability of the microbes in the aquatic product, established by the method, so as to obtain the rotting capability of the microbes in the aquatic product through calculation. By the method, evaluation of the rotting capability in the time span of the whole storage process is performed, and the rotting capabilities of the same spoilage bacterium under different storage conditions can be compared; the method is an effective, comprehensive, convenient, fast and accurate method for evaluating the rotting capability of the microbes, and can be widely applied to the evaluation of the rotting capability of the microbes in the aquatic product.

The invention provides a composite lactobacillus agent for improving grape quality and a preparation method of the composite lactobacillus agent, and belongs to the field of application of microorganisms. The composite lactobacillus agent consists of Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus delbrueckii subsp.bulgaricus in a ratio of (1.5-2):(1.5-2):(1-1.5); the total viable count of the composite lactobacillus agent is 2.4-3.2*10<9>cfu/ml. Compounding of the three types of bacteria has a significant effect of promoting growth and metabolism of grapes, and particularly can synergistically increase the total soluble sugar content and the soluble solid content of the grapes, so that the grape quality is improved and planting benefits are enhanced.

The invention provides a denitrification and algae-removing micro-biological bacterium agent and a preparation method thereof. The denitrification and algae-removing micro-biological bacterium agent provided by the invention is prepared from nitrifying bacteria, nitrite bacteria, denitrifying bacteria, algicidal bacteria and photosynthetic bacteria; the content of the total viable count is not lower than 1.0*10<10>cfu/g. The content of the nitrifying bacteria and the nitrite bacteria accounts for 20 to 30 percent of the total viable count; the content of the denitrifying bacteria accounts for20 to 30 percent of the total viable count; the content of the algicidal bacteria accounts for 30 to 50 percent of the total viable count; the content of the photosynthetic bacteria accounts for 5 to10 percent of the total viable count. The denitrification and algae-removing micro-biological bacterium agent is prepared by mixing various microorganisms according to different ratios and has high viable count; the denitrification and algae-removing micro-biological bacterium agent can be used for biological remediation of a lake and reservoir water body and purification of a eutrophic culture water body and is especially suitable for treating water of a high-algae and high-ammonia-nitrogen micro-polluted water source; the removing effect on pollutants is remarkably better than the removing effect of a single strain; the denitrification and algae-removing micro-biological bacterium agent has the advantages of strong environmental adaptability, no secondary risks, high denitrification andalgae-removing efficiency and the like and belongs to an environment-friendly type micro-biological bacterium agent.

The invention discloses a method for preparing a composite biological bacterial deodorant. The composite biological bacterial deodorant is prepared from efficient ammonia removal strain lactic acid bacteria and saccharomycetes through a special fermentation process through concentration, the total viable count of the deodorant is greater than 2*10<9>cfu/mL, and in addition, the deodorant is rich in active enzyme such as cellulase and protease, is capable of effectively removing substances such as ammonia nitrogen and hydrogen sulfide, is harmless to human bodies and animals, and is a safe andenvironmental-friendly biological bacterial deodorant. The composite biological bacterial deodorant prepared by using the method is sprayed to an odorous environment such as a livestock farm, odorousmolecules dissolved into water are absorbed into microorganisms by the microorganisms of the deodorant and are degraded through metabolism of the microorganisms, meanwhile, organic acids can be also generated from the microorganisms, then an acid environment which is not beneficial to living of deteriorated microorganisms can be formed, and odor caused by metabolism of the deteriorated microorganisms can be substantially avoided.

The invention provides a microbial biocontrol preparation. The microbial biocontrol preparation is a liquid and comprises active ingredients of Trichoderma longibrachiatum and its fermentation metabolites, bacillus amyloliquefaciens and its fermentation metabolites, Rhodopseudomonas palustris and its fermentation metabolites, berberine and chitosan. The microbial biocontrol preparation has a berberine concentration of 0.1 to 0.8 wt% and a chitosan concentration of 0.5-1wt%. The total viable count of the Trichoderma longibrachiatum, bacillus amyloliquefaciens and Rhodopseudomonas palustris is greater than or equal to 5*10<7> cfu/ml. The active ingredients belong to different action mechanisms. Under the synergistic effect of the active ingredients, the microbial biocontrol preparation has good prevention effects on tea grey blight. The active ingredients are environmentally friendly and non-toxic and haves pharmacological and fertilizer dual biological regulation functions.

The invention relates to a functional sugar-free mint which comprises 400-450 g of mixed bacterial powder, 2-2.5 kg of inulin, 18-20 kg of xylitol, 70-75 kg of sorbitol, 500-600 g of natural menthol and 1.5-1.8 g of magnesium stearate, wherein the mixed bacterial powder consists of three types of probiotics of lactobacillus acidophilus, lactobacillus salivarius and lactobacillus reuteri, the total viable count is (2.0-2.3)*10<12>, the viable count of the lactobacillus acidophilus is (1-1.5)*10<12>, the viable count of the lactobacillus salivarius (4-5)*10<11>, and the viable count of the lactobacillus reuteri is (3-4)*10<11>. The probiotic sugar-free mint is obtained by filtering the raw materials respectively through a 30-mesh stainless steel sieve, uniformly mixing the filtered raw materials through a trough mixer, and then conducting wet granulation, drying, granulation and tabletting. The sugar-free mint has the functional characteristics of refreshing breath, being sugar-free and healthy, improving intestinal functions, reducing blood glucose, improving blood lipid condition and the like.

The invention provides an efficient cotton straw decomposition agent which is obtained by fermenting a composite inoculant, and the composite inoculant comprises bacillus subtilis, bacillus velezensis, aspergillus niger, Kazachstania unisporus, saccharomyces cerevisiae and streptomyces cellulosae. The total number of effective viable bacteria in the prepared efficient cotton straw decomposition agent reaches 3.50-6.80 billion/g, decomposition of cotton straw is accelerated by applying the efficient cotton straw decomposition agent and utilizing strains, and the effective degradation rate of dry matter of the cotton straw is increased.