Fusant mixture, preparation method of fusant mixture, and method for producing organic fertilizer

A technology of yeast fusion bacteria and mixed bacteria agent, which is applied in the preparation of organic fertilizers, methods based on microorganisms, organic fertilizers, etc., can solve the problems of large differences in the decomposition speed of organic matter, complex preparation processes, and high costs, and save manpower and energy, simple production process and convenient operation

Active Publication Date: 2015-11-25
GANSU CHANGYE ECOLOGY BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Most of the microbial strains used in the preparation of traditional microbial mixtures are isolated and screened from national or local strain banks or directly from nature. The disadvantages are: 1. The activity of microbial strains is poor; 2. The ability to decompose and transform organic matter Weak; 3. The number of living cells in the mixed bacteria prepared by these strains is small, most of them are 108 / g
Its disadvantages are: complex preparation process, long culture cycle, high cost, poor activity, and limited application
[0004] The traditional method of producing organic fertilizer from straw livestock and poultry is to use the indigenous microorganisms in the raw materials to naturally ferment and degrade the organic matter. However, due to the small number of indigenous microorganisms in the early stage of fertilizer making, these microorganisms take a long time to reproduce, and , and their decomposition speeds of organic matter are also very different. Therefore, the traditional method of making fertilizer from straw livestock and poultry has a long period of time, produces a strong odor, and has low fertilizer efficiency.

Method used

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  • Fusant mixture, preparation method of fusant mixture, and method for producing organic fertilizer
  • Fusant mixture, preparation method of fusant mixture, and method for producing organic fertilizer

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Embodiment 1

[0045] Embodiment 1: a kind of yeast fusion bacteria mixed bacteria agent, it is characterized in that this mixed bacteria agent comprises thalline and culture medium: wherein said thalline comprises yeast fusion bacteria (Fusantbetween CandidatropicalisandSaccharmycescevisiae) F105, in China Microorganism Strain Preservation Management Committee Preserved by the General Microorganism Center, the address of the depository unit is: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing. The preservation time is December 30, 2014, and the preservation number is CGMCCNO.10263; Bacillus Licheniformis B-36 was purchased from the China Agricultural Microorganism Culture Collection Center with the number Accc11080; Azotobacter chroococum LGN-09 was preserved in the General Microbiology Center of the China Microbiology Culture Collection Management Committee. The address of the preservation unit is: Chaoyang District, Beijing The In...

Embodiment 2

[0046]Embodiment 2: described yeast fusion bacteria mixed bacteria agent also includes mixed bacteria liquid culture medium and adds ammonium sulfate 1-2g in one liter of fresh bean curd wastewater or vermicelli wastewater or cornstarch wastewater or potato starch wastewater, calcium carbonate 1 -2g, phosphoric acid 0.5-1ml, adjust the pH to 6.2-7.5 with 1% lime water, divide into 500ml Erlenmeyer flasks, the filling volume is 1 / 4 of the capacity of the bottle, then heat through 110-112°C for 30-35 minutes Autoclave, after cooling, get the agar slant test tube strains of yeast fusion bacteria, Bacillus licheniformis, Azotobacter rufosa, Streptomyces and Trichoderma konii respectively, and transfer them to the liquid Erlenmeyer flask culture medium under aseptic conditions respectively. After standing at 28-32°C for 24-72 hours or shaking culture on a shaking table, or aeration and stirring fermentation culture in a fermenter, the fermentation broth is prepared according to its ...

Embodiment 3

[0047] Embodiment 3: the preparation of a kind of yeast fusion bacterium has the following steps: the slant planting and cultivation of alcoholic yeast as the mother species, after 14 hours of shaking culture in liquid medium, carry out pretreatment with EDTA-mercaptoethanol, use 1.5 of cellulase and 0.5 of helicase to enzymatically detach the wall, the time is 2.5 hours, the temperature is 33 ° C, and the protoplasts of alcoholic yeast are obtained; the slant of Candida tropicalis as the mother species is transferred and cultivated: in liquid medium After shaking and culturing for 14 hours, pretreat with EDTA-mercaptoethanol, and use 1.5% cellulase and 0.5% helicase to enzymatically detach the wall for 2.5 hours at a temperature of 33°C to obtain protoplasts of Candida tropicalis; Candida protoplasts were inactivated by 0.1% iodoacetic acid, and then mixed with alcoholic yeast protoplasts at a ratio of 1:1, and the precipitate was suspended in 35% polyethylene glycol (PEG) sol...

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Abstract

The invention relates to a fusant mixture, a preparation method of the fusant mixture, and a method for producing bio-organic fertilizer by fermenting straw and livestock and poultry manure. The fusant mixture is mainly characterized by comprising a thallus and a culture medium, wherein the thallus comprises yeast fusant F105, bacillus licheniformis B-36, native azotobacter chroococcum A-7, streptomyces S-11 and T.reesi T-2; the viable count of the fusant is 25-40 percent of that of the total viable count; the viable count of the bacillus licheniformis is 15-30 percent of the total viable count; the viable count of the native azotobacter chroococcum is 15-30 percent of the total viable count; the viable count of the streptomyces is 15-30 percent of the total viable count; and the viable count of the T.reesi is 10-35 percent of the total viable count.

Description

technical field [0001] The invention relates to a yeast fusion bacteria mixed agent and a preparation method thereof, and a method for producing bio-organic fertilizer by fermenting straw, livestock and poultry feces. Background technique [0002] Most of the microbial strains used in the preparation of traditional microbial mixtures are isolated and screened from national or local strain banks or directly from nature. The disadvantages are: 1. The activity of microbial strains is poor; 2. The ability to decompose and transform organic matter Weak; 3. The number of living cells in the mixed bacteria prepared by these strains is small, most of them are 108 / g [0003] The traditional preparation method of microbial mixture agent is to cultivate various strains (aerobic or anaerobic) in different growth media, and then scrape the lawn from the culture medium of each strain to make The liquid bacterial suspensions of various strains are replanted in their respective liquid medi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/19C12N1/14C12N15/04C12N15/01C12N13/00C05F17/00C05F15/00C12R1/865C12R1/74C12R1/10C12R1/065C12R1/465C12R1/885
CPCY02W30/40
Inventor 孙荣高代积文代凤雷曾学平高小青
Owner GANSU CHANGYE ECOLOGY BIOTECH CO LTD
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