7 results about "Iodoacetic acid" patented technology

The present invention relates to the preparation and application of a micro-cell in-situ microreactor. By subjecting the inner wall of a capillary to silanization treatment and modifying epoxy groups on its surface through free radical polymerization reaction, an open-tubular capillary column is prepared. Then, polyethyleneimine and iodoacetic acid-N-succinimidyl ester are successively modified by covalent bonding to prepare a solid-phase alkylated capillary microreactor. The advantages of the present invention are that proteomic sample processing is carried out in-situ, which is conducive to reducing sample loss caused by multiple transfers during traditional sample processing. Therefore, this solid-phase alkylated open-tubular column is suitable for proteomic pretreatment of trace samples. In addition, this method is tolerant to various surfactants and strong lysis reagents and has good sample compatibility.

This invention relates to a fractured solid-phase alkylation material, its preparation, and its application. Using epoxy-based microspheres as a carrier, polyethyleneimine, an acid-fractureable linker arm, and iodoacetic acid-N-succinamide ester are sequentially modified via covalent bonding to prepare the fractured solid-phase alkylation material. This material selectively reacts with thiol groups on proteins, enabling efficient enrichment and processing of proteins in complex biological samples such as cells, tissues, and body fluids. By cleaving the linker arm under acidic conditions, the protein and peptide fragments can be released without damage, potentially providing important technical support for deep proteomic coverage analysis and protein variant analysis.

The invention discloses a method for synthesizing jasmine lactone through photocatalytic reaction, and belongs to the field of fine chemicals. The synthesis method comprises the following steps: taking (E)-1, 6-octadiene-3-ketone-2 and iodoacetic acid as raw materials, carrying out photocatalytic reaction to obtain (E)-5-oxo-8-decenoic acid, then reacting with sodium borohydride to obtain (E)-5-hydroxy-8-decenoic acid, and carrying out esterification reaction on a crude product under the catalysis of acid without separation to obtain jasmonlactone. The method for obtaining the jasmine lactone has the advantages of easiness in synthesis of raw materials, mild reaction conditions, low cost and few steps, shows the superiority and simplicity of the synthesis method, and provides a simple way for preparation of the compounds.

The application discloses a solid-phase extraction membrane based on MOF material and a preparation method and application thereof, and the solid-phase extraction membrane comprises a carrier and an enrichment medium coating loaded on the surface of the carrier; the material of the enrichment medium coating is a defective amino MOF material; the defective amino MOF material is a metal organic framework material prepared by taking zirconium as a metal center, taking dichloroacetic acid as a modulator and reacting with an amino organic ligand; and the carrier is an organic filter membrane. The adsorbent used in the coating of the solid-phase extraction membrane is a defective MOF material prepared by induction of dichloroacetic acid, and the material has the advantages of large adsorption capacity and good chemical stability; the solid-phase membrane coating prepared by taking the material as raw material has the advantages of good water resistance, high enrichment multiple and good reproducibility, and has excellent adsorption effect on bromoacetic acid and iodoacetic acid.

The invention provides a construction and evaluation method of a porcine retinitis pigmentosa disease model, and belongs to the technical field of animal models of human diseases. The invention provides a method for constructing and evaluating a porcine retinitis pigmentosa disease model, which comprises the following steps: applying iodoacetic acid to the vein of a miniature pig to construct the porcine retinitis pigmentosa disease model; after the iodoacetic acid is applied to the veins of the miniature pig, the miniature pig is subjected to ophthalmic examination; the ophthalmic examination comprises eye morphological structure and function examination. According to the construction method, iodoacetic acid is used as a modeling reagent, directional damage to the miniature pig retina photoreceptor cells can be rapidly achieved within one week, and the modeling rate is high. Moreover, after the iodoacetic acid is applied to the veins of the miniature pig, the miniature pig is subjected to ophthalmic examination including eye morphological structure and function examination, analysis is comprehensive, and the constructed model is stable, reliable, good in uniformity, long in maintenance time and easy to copy.

The invention relates to an extracellular vesicle in-situ microreactor and preparation and application thereof, the inner wall of a capillary is subjected to silanization treatment, and the surface of the capillary is modified with epoxy groups and amphoteric molecules through free radical polymerization reaction, so that a capillary open tubular column is prepared; and then modifying polyethyleneimine and iodoacetic acid N-hydroxysuccinimide ester in sequence through covalent bonding to prepare the amphoteric solid-phase alkylation capillary microreactor. The method has the advantages that the extracellular vesicles are captured in situ, the vesicle proteome sample is treated, and the method is suitable for proteome sample treatment of trace extracellular vesicle samples. In addition, the method is resistant to various surfactants and strong cracking reagents and has good sample compatibility.

The invention relates to a breakable solid-phase alkylation material as well as preparation and application thereof, epoxy microspheres are taken as a carrier, polyethyleneimine, an acid breakable connecting arm and iodoacetic acid-N-succinamide ester are sequentially modified in a covalent bonding manner, and the breakable solid-phase alkylation material is prepared. The material selectively reacts with sulfydryl on protein, efficient enrichment and treatment of protein in complex biological samples such as cells, tissues and body fluid can be achieved, lossless release of protein and peptide fragments can be achieved by breaking connecting arms under the acidic condition, and important technical support is expected to be provided for proteome deep coverage analysis and protein variant analysis.