3 results about "Iodoacetic acid" patented technology

This invention relates to a fractured solid-phase alkylation material, its preparation, and its application. Using epoxy-based microspheres as a carrier, polyethyleneimine, an acid-fractureable linker arm, and iodoacetic acid-N-succinamide ester are sequentially modified via covalent bonding to prepare the fractured solid-phase alkylation material. This material selectively reacts with thiol groups on proteins, enabling efficient enrichment and processing of proteins in complex biological samples such as cells, tissues, and body fluids. By cleaving the linker arm under acidic conditions, the protein and peptide fragments can be released without damage, potentially providing important technical support for deep proteomic coverage analysis and protein variant analysis.

The invention discloses a method for synthesizing jasmine lactone through photocatalytic reaction, and belongs to the field of fine chemicals. The synthesis method comprises the following steps: taking (E)-1, 6-octadiene-3-ketone-2 and iodoacetic acid as raw materials, carrying out photocatalytic reaction to obtain (E)-5-oxo-8-decenoic acid, then reacting with sodium borohydride to obtain (E)-5-hydroxy-8-decenoic acid, and carrying out esterification reaction on a crude product under the catalysis of acid without separation to obtain jasmonlactone. The method for obtaining the jasmine lactone has the advantages of easiness in synthesis of raw materials, mild reaction conditions, low cost and few steps, shows the superiority and simplicity of the synthesis method, and provides a simple way for preparation of the compounds.

The invention relates to an extracellular vesicle in-situ microreactor and preparation and application thereof, the inner wall of a capillary is subjected to silanization treatment, and the surface of the capillary is modified with epoxy groups and amphoteric molecules through free radical polymerization reaction, so that a capillary open tubular column is prepared; and then modifying polyethyleneimine and iodoacetic acid N-hydroxysuccinimide ester in sequence through covalent bonding to prepare the amphoteric solid-phase alkylation capillary microreactor. The method has the advantages that the extracellular vesicles are captured in situ, the vesicle proteome sample is treated, and the method is suitable for proteome sample treatment of trace extracellular vesicle samples. In addition, the method is resistant to various surfactants and strong cracking reagents and has good sample compatibility.