18 results about "Candida tropicalis" patented technology

The invention relates to the technical field of cosmetics, and particularly discloses an anti-hair-loss composition as well as a preparation method and application thereof. The invention relates to a preparation method of an anti-hair loss composition, which comprises the following steps: cleaning and drying a fermentation substrate, crushing into slurry, sterilizing, inoculating lactobacillus plantarum, candida tropicalis, schizosaccharomyces cerevisiae and monascus purpureus strains at a proper temperature, carrying out sealed fermentation, and after fermentation is completed, carrying out centrifugal separation on the fermentation liquor to obtain the anti-hair loss composition. Taking supernate, sterilizing, and performing vacuum freeze drying to obtain the anti-alopecia composition. According to the application of the anti-hair-loss composition, the anti-hair-loss composition is added into a hair product. The anti-hair loss composition disclosed by the invention is natural in source, and can promote absorption of skin to active substances, so that the anti-hair loss composition has the effects of rapidly inhibiting scalp oil and reducing hair loss.

The invention provides a preparation method and application of alpinia oxyphylla leaf essential oil, and relates to the field of natural plant extracts. The alpinia oxyphylla leaf essential oil is applied to preparation of a bacteriostatic agent for inhibiting bacteria or / and fungi, and the bacteria comprise one or more of klebsiella pneumoniae, salmonella enterobacter and shigella flexneri. The fungi comprise one or more of candida tropicalis and malassezia furfur; the preparation method of the alpinia oxyphylla leaf essential oil comprises the following steps that alpinia oxyphylla leaves are taken, dried and smashed, the smashed alpinia oxyphylla leaves are placed in water to be soaked, the material-liquid mass ratio is 1: (15-17), and the soaking time is 0.8-1.2 h; after soaking, distillation is carried out, the distillation time is 4.5-5.5 h, and the distillation temperature is 170-200 DEG C; and after distillation is finished, collecting upper oily liquid to obtain the alpinia oxyphylla leaf essential oil. The prepared alpinia oxyphylla leaf essential oil has broad-spectrum antibacterial and bactericidal effects, and the antibacterial and bactericidal effects are good.

The application provides a water-retaining soil conditioner containing high-efficiency degrading bacteria and a preparation method and application thereof, and belongs to the technical field of soil improvement.The water-retaining soil conditioner comprises a carboxymethylated modified humic acid-chitosan compound, modified attapulgite powder, a composite microbial inoculum, konjac glucosan, polyacrylic acid, bentonite, polyglutamic acid, sodium alginate, mushroom residue, seaweed residue and straw compost; the composite microbial inoculum is obtained by combination of Candida tropicalis, Bacillus smithii and Bacillus pasteurii.The application realizes precise adaptation of the carrier and the high-efficiency degrading bacterial group by optimizing the design of the composite water-retaining carrier and introducing specific bacterial combinations, strengthens the synergistic effect of water retention, bacteria fixation, slow release and soil improvement, realizes water retention, degradation and fertilization synergistic effect, and has important practical significance and application value for improving soil quality, guaranteeing crop production and promoting the development of ecological agriculture.

This invention discloses a plant essential oil composition with anti-Candida activity and its preparation method, which consists of a heavy oil component of Sichuan pepper and a light oil component of dried ginger in a volume ratio of 1:8 to 2:1. Research in this invention has found that, after specific volume ratios, particularly when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 1:8, it exhibits a synergistic effect against Candida albicans, Candida tropicalis, and Candida glabrata; when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 2:1, it exhibits a very good synergistic effect against Candida parapsilosis. This invention allows the heavy oil component of Sichuan pepper and the light oil component of dried ginger to be formulated into an anti-Candida drug in a specific volume ratio.

The invention discloses a candida tropicalis engineering bacterium for producing 1, 2, 4-butantriol as well as a construction method and application of the candida tropicalis engineering bacterium, and belongs to the technical field of microorganisms. According to the recombinant candida tropicalis strain constructed by the invention, scaffold protein ligand-enzyme fusion protein is subjected to integrated expression through metabolic engineering transformation, and a scaffold protein structural domain GBD-SH3-PDZ is overexpressed on the basis of the scaffold protein ligand-enzyme fusion protein; a glucose repression gene MIG1 gene is knocked out; the KdcA mutant is subjected to overexpression, the yield of 1, 2, 4-butantriol can be obviously increased, the shake flask yield of 1, 2, 4-butantriol is increased to 2.1 g / L from 0.6 g / L of the original strain BTO-01, the yield is increased by 3.35 times, and the yield of the strain BTO-14 in a 5L tank reaches 11.18 g / L through amplification culture of a fermentation tank.

The present application relates to a kind of Candida tropicalis engineering bacteria and its preparation method and the method for treating sewage, belong to genetic engineering technical field.Candida tropicalis engineering bacteria with Candida tropicalis CBW-2 ΔURA3 (preservation number: CGMCC NO.31647) as chassis strain, knock out ACS gene and transfer into promoter heterologous expression alpha-amylase gene;The promoter is endogenous promoter.Candida tropicalis is a kind of unconventional yeast with industrial significance, with the characteristics of fast cell growth, strong environmental tolerance, has the potential of sewage treatment.The present application significantly reduces the activity of acetyl-coenzyme A synthetase by knocking out ACS gene, increases the acetic acid accumulation of strain.And by high-efficiency expression alpha-amylase, starch component can be efficiently converted into acetic acid, can provide acetic acid as carbon source for heterotrophic nitrification-aerobic denitrification strain, without adding exogenous carbon source.

The invention provides a Candida tropicalis ERG11 gene copy number variation digital PCR (polymerase chain reaction) detection method, the total copy number of ERG11 genes is detected through a group of universal primers, a wild type probe of an A395 site of the ERG11 genes in a detection sample and a mutant type probe of the A395 site of the ERG11 genes, and the total copy number of the ERG11 genes is equal to the sum of the copy number of wild target spots and the copy number of mutant target spots; in the PCR detection method, the copy number of the wild target and the copy number of the mutation target are corrected by using the copy number of two reference genes. Compared with an ERG11 gene mutation and copy number variation detection method based on a fluorescent quantitative PCR method, the accuracy of copy number variation detection is greatly improved, and the problem of pain points of tandem sequence copy number recognition and quantitative detection in the candida tropicalis drug resistance detection process can be effectively solved.

The invention discloses a candida tropicalis engineering bacterium for producing astaxanthin, and belongs to the field of metabolic engineering. Candida tropicalis ANT-06 is used as a starting strain to integrally express a heterologous gene CrtZ and a gene CrtW, the heterologous gene CrtZ and the gene CrtW are subjected to fusion expression with a peroxisome signal peptide ePTS and a lipid droplet signal peptide HD2, and the yield of astaxanthin can be obviously increased through a combined strategy of knocking out a phosphatidic acid phosphatase gene PAH1 and a functional homolog gene FLD1 of human seipin. And after combined fermentation optimization, fermentation culture at 22 DEG C and addition of 10% of dodecane, 2.5% of DMSO and 2.5 mM of betaine, the shake flask fermentation yield of the astaxanthin is increased to 320.1 mg / L, and the amplification fermentation yield of a 5L fermentation tank is increased to 3096.2 mg / L. The preparation method has the characteristics of low cost, high efficiency, simplicity in operation, wide prospect and the like.

The invention discloses application of a Candida tropicalis strain (Candida tropicalis) to degradation of TNT (trinitrotoluene), and further provides a screening method of the Candida tropicalis strain (Candida tropicalis). The invention also provides an application of the TNT efficient degradation strain. According to the present invention, the Candida tropicalis strain can tolerate more than 400 ppm of TNT, can complete the efficient degradation of TNT within 10 h, can effectively solve the problems of low tolerance concentration, slow metabolism rate and the like of the existing TNT metabolism microorganisms, and is mainly used for solid TNT microbial conversion, TNT wastewater treatment and in-situ purification of TNT contaminated soil.

The invention discloses a method for preparing a soil conditioner through rapid directional fermentation and alcohol extraction of panax notoginseng residues and the soil conditioner, and belongs to the technical field of preparation of soil conditioners through fermentation of traditional Chinese medicine residues. Comprising the steps that traditional Chinese medicine residues are inoculated with strains to be fermented to prepare the soil conditioner, the traditional Chinese medicine residues are alcohol-extracted pseudo-ginseng residues, the strains comprise bacillus subtilis B257, pseudomonas monteilii PM-41, myrothecium verrucaria, pyrethrum umbellioides, bacillus licheniformis and candida tropicalis, and fermentation comprises the steps of S1 high-temperature detoxification and S2 medium-temperature humification. By designing a composite strain combination and fully considering the compatibility with alcohol-extracted pseudo-ginseng dregs, the strains are scientifically coordinated. The strains are put in batches for directional continuous fermentation in two stages, the ineffective transition period of a traditional process is eliminated, the fermentation period is shortened to about 7 days, efficient conversion of medicine residues is achieved, energy consumption is reduced, the obtained soil conditioner is high in humic acid content and excellent in fertilizer efficiency, and resource utilization of alcohol extraction medicine residues is achieved.

The present invention relates to microbiological methods and media dedicated for the non-denaturing detection, differentiation and / or identification of Candida spp. Yeasts, in particular Candida auricula, Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis and Candida krusei. The invention more specifically relates to a method for identifying a yeast of the genus Candida, optionally present in a biological sample, comprising the steps of: culturing the yeast to be identified on or in a culture medium comprising:-a nutritional ingredient capable of allowing the development and growth of a yeast of the genus Candida; and-an alpha-glucosidase inducer and, optionally, an alpha-glucosidase repressor; -an acid phosphatase inducer and a phosphatase repressor; a-N-acetyl-beta-glucosaminase inducer, and a method for producing the same; and-detecting any alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase activity expressed by said yeast; and-identifying the species to which said yeast belongs, based on the activity of alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase actually expressed by said yeast.

The invention belongs to the technical field of synthesis of sodium 8-(2-hydroxybenzamido) caprylate (SNAC), and particularly relates to a method for biologically synthesizing SNAC, which comprises the following steps: inoculating yeast to a fermentation medium, adding 2-hydroxy-N-octyl benzamide and a salt-forming agent, controlling the pH value of the fermentation medium to be alkaline, and carrying out biological fermentation to synthesize the SNAC, wherein the yeast is selected from one of candida tropicalis, candida maltose and yarrowia lipolytica. According to the method, a biological method and a chemical method are organically fused, respective advantages of the biological method and the chemical method are fully played, a brand new SNAC synthesis route mainly depending on microbial fermentation is innovatively developed, green synthesis of the SNAC is realized by utilizing a microbial cell factory, the synthesis route of the SNAC is greatly shortened, and the method has the advantages of cheap and easily available raw materials, lower cost, mild reaction conditions, high yield and the like. And the method has the advantages of low environmental pollution, high atom economy, green production and the like.

The present application belongs to the technical field of waste recycling, and particularly relates to a method for recycling beer lees. The method is as follows: after the beer lees are extruded and puffed, Candida tropicalis SCYA4 bacterial liquid is added for fermentation, the Candida tropicalis is preserved in the China Center for Type Culture Collection on May 23, 2024, and the preservation number is CCTCC NO: M20241049. By using the Candida tropicalis SCYA4 to ferment after the beer lees are extruded and puffed, the present application can significantly improve the digestion efficiency of animals on the nutrients in the beer lees.

The present invention relates to a composition for the treatment of fungal infections of C. albicans and C. non-albicans, such as C. auris, C. glabrata, C. tropicalis, C. krusei and Trichophyton spp., in particular Trichophyton mentagrophytes complex, Tricophyton rubrum.

The invention belongs to the technical field of synthesis of sodium 8-(2-hydroxybenzamido) caprylate (SNAC), and particularly relates to recombinant candida tropicalis and application thereof. The preservation number of the recombinant candida tropicalis is CCTCC (China Center For Type Culture Collection) NO: M 20253005. The recombinant candida tropicalis suitable for SNAC biosynthesis is obtained through screening and genetic modification and is introduced into SNAC synthesis, 2-hydroxy-N-octyl benzamide is converted into 8-(2-hydroxybenzamido) octanoic acid through biological fermentation of the recombinant candida tropicalis in the synthesis route, and the 8-(2-hydroxybenzamido) octanoic acid is converted into 8-(2-hydroxybenzamido) octanoic acid. And salifying the 8-(2-hydroxybenzamido) octanoic acid while the substrate is converted, so as to synthesize the SNAC. The method provided by the invention adopts cheap and easily available raw materials, is mild in reaction condition, easy to control, high in reaction selectivity and specificity, good in biological safety and small in environmental pollution, and has the potential of large-scale industrial application.

The invention discloses a fermented feed for promoting ruminants to efficiently utilize soluble proteins as well as a preparation method and application of the fermented feed, and belongs to the technical field of animal feeds. The fermented feed is prepared by fermenting a substrate consisting of cottonseed meal, corn flour and bran with a compound bacteria solution, and the substrate comprises the following components in percentage by mass: 5-31% of cottonseed meal, 2-31% of corn flour and 38-93% of bran; the mass of the composite bacterial liquid is 8-10% of that of the substrate, and the composite bacterial liquid is prepared by compounding a candida tropicalis bacterial liquid and an enterococcus faecalis bacterial liquid according to a volume ratio of 1: 1, wherein the concentrations of the candida tropicalis bacterial liquid and the enterococcus faecalis bacterial liquid are both 108 CFU / mL. The preparation method comprises the following steps: crushing and sieving the substrate, mixing, adjusting the water content to 40%, spraying the compound bacteria liquid, uniformly mixing, and fermenting at a constant temperature of 30 DEG C. According to the invention, the substrate ratio and the fermentation process are optimized, the utilization efficiency of soluble protein by ruminants is improved, the rumen fermentation environment is improved, meanwhile, the nitrogen emission is reduced, and economic benefits and environmental protection values are achieved in animal production.

The invention relates to the technical field of soil remediation, in particular to a soil remediation agent for petroleum-contaminated soil and a preparation method of the soil remediation agent, and aims to solve the problem that existing petroleum-contaminated soil is difficult to naturally remedy. According to the preparation method, the polluted soil is repaired by utilizing a bioremediation method, the advantages of high efficiency, good effect and small environmental damage are achieved by adding florae into the polluted soil to remove petroleum hydrocarbon pollutants, the candida tropicalis and the streptococcus faecalis are adopted for composite treatment, the candida tropicalis has the advantages of high permeability resistance, acid resistance and high metabolism efficiency of fungi, and the streptococcus faecalis and the candida tropicalis have the advantages of being high in metabolism efficiency. Streptococcus faecalis can be degraded by the petroleum hydrocarbon, pseudomycelium and spores can be generated, an extended network structure is formed in soil and can be used as a channel for bacterial migration, bacterial migration is facilitated, the treatment effect on pollutants is improved, streptococcus faecalis can grow in the environment with the petroleum hydrocarbon, the degradation effect on the petroleum hydrocarbon is good, and the degradation effect on the petroleum hydrocarbon is good. By compounding the two components, a good biodegradation effect can be achieved.