98 results about "Candida tropicalis" patented technology

The invention relates to the field of microorganisms, in particular to a plant lactobacillus and application thereof in bacteriostasis. Specifically, the preservation number of the plant lactobacillus LP06 is CGMCC (China General Microbiological Culture Collection Center) No. 28412. The plant lactobacillus LP06 provided by the invention can be used for inhibiting the growth of debaryomyces hansenii, candida tropicalis and penicillium rochei, and also has an inhibiting effect on super bacteria acinetobacter baumannii, pseudomonas aeruginosa, klebsiella, escherichia coli, staphylococcus aureus and listeria monocytogenes. The plant lactobacillus LP06 provided by the invention has broad-spectrum bacteriostatic ability, and bacteriostatic powder prepared from the bacterium can be used as a biological preservative for corrosion prevention of dairy products such as yoghourt, cheese and the like and other foods.

The invention discloses candida tropicalis with the effect of reducing nitrite and ammonia nitrogen and application of the candida tropicalis. The saccharomycetes are respectively named as candida tropicalis JM-2, and the preservation number of the candida tropicalis JM-2 is GDMCC No: 64449. The saccharomyces cerevisiae is high in adaptability, grows well in high-concentration ammonia nitrogen and nitrite environments, is relatively high in nitrogen degradation efficiency, can be used as a functional strain for improving water quality, deodorizing farms, deodorizing garbage yards and the like, can be applied to a plurality of places, effectively degrades harmful substances such as nitrite, ammonia nitrogen and the like in water bodies and environments, reduces nitrogen accumulation, protects the environments, and has a wide application prospect. The method has great application potential in livestock and poultry aquaculture and environmental protection.

The invention provides application of candida tropicalis and a preparation thereof in prevention and treatment of tobacco mildew, and belongs to the technical field of microorganisms. A strain of candida tropicalis is named as candida tropicalis 2-17, the preservation number of the candida tropicalis 2-17 is CCTCC NO: M 20242184, a microbial preparation prepared from the candida tropicalis can inhibit growth of main mould in tobacco leaves, and the candida tropicalis has good application potential for biological prevention and treatment of tobacco leaf mould.

The invention discloses a urinary system infection pathogen and drug resistance gene detection system and a kit. The detection system comprises forward and reverse primers which are respectively used for detecting escherichia coli, staphylococcus aureus, acinetobacter baumannii, klebsiella pneumoniae, enterococcus faecium, candida glabrata, proteus mirabilis, enterococcus faecalis, candida tropicalis, enterobacter cloacae, candida albicans, pseudomonas aeruginosa and streptococcus agalactiae; the forward primer and the reverse primer are respectively used for detecting the blaCTX-M gene of the extended spectrum beta-lactamase, the forward primer and the reverse primer are used for detecting the blaSHV gene of the extended spectrum beta-lactamase, and the forward primer and the reverse primer are used for detecting the blaTEM gene of the extended spectrum beta-lactamase. According to the forward and reverse primers for detecting the blaNDM gene of the extended-spectrum beta-lactamase, a detection sample is urine. According to the invention, common gram-negative bacteria, gram-positive bacteria and fungi can be detected at the same time, and the problem of missed detection of mixed infection in traditional culture is solved.

The invention provides a microbial composition and application thereof in preparation of selenium-rich sleep-aiding goat milk powder, and belongs to the technical field of microbial fermentation. The microbial composition disclosed by the invention comprises bifidobacterium animalis, enterococcus durans and candida tropicalis. A food additive obtained by fermenting Chinese yam, selenium-rich black beans, Chinese wolfberry fruits, licorice roots, wheat and tremella fuciformis through the microbial composition has the sleep aiding effect, milk powder prepared by combining the microbial composition with other raw materials has the remarkable sleep improving effect, and the milk powder can be taken for a long time and is safe, nutritional and healthy.

The present invention relates to an enzyme catalyst for preparing a dicarboxylic acid and a method for preparing a dicarboxylic acid using the same. The enzyme catalyst is prepared by a method comprising the following steps: (1) carrying out fermentation culture on enzyme-producing microorganisms in a culture medium to obtain a crude enzyme solution, and (2) immobilizing the crude enzyme solution on a molecular sieve to obtain the enzyme catalyst, wherein the enzyme producing microorganisms are selected from candida tropicalis, candida sake, pichia pastoris, saccharomyces cerevisiae, lactobacillus brevis, corynebacterium glutamicum, bacillus subtilis, bacillus cereus, escherichia coli and pseudomonas aeruginosa. The method comprises the following steps: in a PBS buffer solution, mixing triglyceride with the enzyme catalyst, and carrying out catalytic reaction under stirring at 20-50 DEG C; and then carrying out hydrolysis reaction at the temperature of 250-300 DEG C under the pressure of 30-100 bar. According to the method, the enzyme catalyst and the molecular sieve are immobilized, so that other oxidants or catalysts are not required to be added while the dicarboxylic acid with higher yield is obtained.

The invention relates to the technical field of biological medicine, in particular to application of a combination of a Cd1 and Mdr1 double-target inhibitor and an azole drug in preparation of an antifungal drug. Based on the fact that the compound as shown in the formula (I) can be used as a CCd1 and Mdr1 double-target inhibitor, the inhibition effect of the compound on candida albicans, candida auricula, candida glabrata, candida tropicalis, trichophyton rubrum and trichophyton indicus when the compound is combined with azole drugs is further evaluated, and the result shows that the compound as shown in the formula (I) has no obvious antibacterial activity on various fungi, and can be used for preparing a medicine for treating various fungi. The drug resistance of CCd1 and Mdr1 overexpression drug-resistant fungi can be reversed, the activity of Candida albicans, Candida auricula, Candida glabrata, Candida tropicalis, Trichophyton rubrum and Trichophyton indicus can be synergistically inhibited when the drug is combined with azole drugs, the treatment dosage of the azole drugs is effectively reduced, and the drug toxicity is small. Therefore, the compound shown in the formula (I) can improve the antifungal effect of azole drugs and reduce the drug resistance of the azole drugs.

The invention discloses Candida tropicalis, and the preservation number of the Candida tropicalis in the Guangdong Microbial Culture Collection Center is GDMCC No: 64869. The Candida tropicalis has the advantages that the preservation number of the Candida tropicalis is CGMCC No: 64869; the invention further provides application of the candida tropicalis in fermentation production of single-cell protein, a culture medium suitable for the candida tropicalis and a method for fermentation production of the single-cell protein. The candida tropicalis is a high-stress-resistance strain of vinegar residue hydrolysate, is suitable for large-scale production, can effectively promote recycling of vinegar residue waste biomass resources, and relieves the current situation of protein feed shortage in the breeding industry to a certain extent.

The invention discloses candida tropicalis engineering bacteria for high yield of (-)-alpha-bisabolol and a construction method of the candida tropicalis engineering bacteria, and belongs to the technical field of microorganisms. According to the invention, chassis cell candida tropicalis DC04-WG04-2 is constructed, a gene Cat2 of catalase derived from saccharomyces cerevisiae and a gene ERG10 of 3-hydroxy-3-methylglutaryl coenzyme A reductase are expressed on the basis of the chassis cell candida tropicalis DC04-WG04-2, and a PEX15 motif is anchored on the surface of peroxisome for co-expression; the genes ADH1, GPD1 and GPD2 are further knocked out, so that the accumulation of ethanol is reduced, and the generation of glycerol is reduced. According to the method disclosed by the invention, the yield of the (-)-alpha-bisabolol of the recombinant candida tropicalis reaches 461.7 mg / L by optimizing fermentation conditions. The preparation method has the characteristics of low cost, high efficiency, simplicity in operation, wide prospect and the like.

The present invention relates to a strain of Candida and its application, belonging to the technical field of microbial fermentation. The Candida SY152 provided by the present invention is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration Committee, with a deposit number of CGMCC No.31427, a deposit date of July 24, 2024, a deposit address of Beijing, China, and is classified and named as tropical Candida Candida tropicalis The Candida SY152 provided by the present invention can increase the pH value of the culture medium, and when used for fermentation of soybean paste or soy sauce, it can significantly reduce the total acid content in the soybean paste or soy sauce, while increasing the ethanol content, thereby increasing the generated alcohol and ester substances, thereby enhancing the mellow flavor of the finished soybean paste or soy sauce, and significantly improving its taste and flavor.

The invention relates to candida tropicalis and application thereof, the candida tropicalis is a candida tropicalis JSY83 strain, the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20241913, and the preservation date is September 4, 2024. The strain has excellent ethanol, acetic acid and lactic acid tolerance, especially has more excellent lactic acid tolerance, and ensures the growth and functional effects of the strain in the actual white spirit brewing process; by inhibiting the growth of ester-producing yeast, reducing the accumulation of acetic acid, reducing the content of acetic acid in fermented grains and inhibiting the accumulation of ethyl acetate in the fermented grains, the method has important significance on improving the high-quality product rate and the liquor yield of raw liquor when being used for producing yeast for making hard liquor and Luzhou-flavor liquor.

The present invention discloses a pyruvate decarboxylase mutant with improved catalytic efficiency and its application. The present invention constructs a pyruvate decarboxylase mutant derived from Candida tropicalis for catalytic production of 4-hydroxyphenylacetaldehyde. The catalytic half-life (5.7 h) and TTN (24,600) of the mutant of the present invention are increased by 2.85 times and 2.07 times respectively compared with the control, and the mutant exhibits high decarboxylation activity. When the obtained mutant of the present invention uses 4-hydroxyphenylpyruvic acid as a substrate and reacts for 12 h at 25 °C and pH 6.5, the yield of 4-hydroxyphenylacetaldehyde can reach 5.08 g / L, and the molar conversion rate of the substrate reaches 67.28%. By adopting the method of the present invention, the production capacity of the unit catalyst and the reaction efficiency of the catalyst are improved, and the industrialization process of producing 4-hydroxyphenylacetaldehyde by the enzyme conversion method is accelerated.

This application belongs to the field of biology and discloses a strain of Candida tropicalis, which is classified and named candida tropicalis The deposit number is GDMCC NO: 65030; the deposit date is August 20, 2024, and it is deposited at the Guangdong Provincial Center for Microbiological Culture Collection, with the deposit address being: 5th Floor, Building 59, No. 100, Xianlie Middle Road, Guangzhou. When fermented with this tropical yeast, Astragalus polysaccharide can enhance the immune-enhancing effect of Astragalus polysaccharide, stimulate animals to produce higher antibody titers after immunization, and reduce adverse immune reactions. The present invention also provides an Astragalus polysaccharide fermentation product, a preparation method, and applications.

The invention belongs to the technical field of vegetable oil extraction, and discloses an efficient fermentation preparation method of Badam vegetable oil based on synergistic metabolism and dynamic regulation of composite flora, Badam fermented oil and application. According to the core scheme of the preparation method, the innovative process for efficiently converting the almond kernel residues into the high-activity vegetable oil is realized through the synergistic effect of composite flora (candida tropicalis, aspergillus niger and lactobacillus plantarum) in combination with a three-stage dynamic dissolved oxygen regulation and control, a pulse feeding strategy and a membrane separation-molecular distillation combined technology; the preparation method disclosed by the invention can be used for remarkably improving the grease yield and the retention rate of functional components, and is suitable for high-added-value grease production in food, medicine and cosmetic industries.

The invention belongs to the technical field of biology and the technical field of fermentation, and relates to a compound microbial agent and application thereof in regulating and controlling the fermentation temperature of a saccharification pile. The compound microbial agent is prepared from bacillus coagulans, bacillus subtillis, pichia kudriavzevii, candida tropicalis, saccharomyces cerevisiae, high-temperature Daqu actinomycetes and common high-temperature actinomycetes, and the compound microbial agent is used for saccharification and stacking fermentation, so that the stacking fermentation temperature is increased, and the stacking time is shortened.

The invention provides a method and a kit for detecting blood flow infection pathogens and application of the method and the kit. Specifically, the invention provides a primer probe combination for detecting bloodstream infection pathogens, and the primer probe combination comprises: (a) upstream and downstream primers and probes for detecting candida as shown in SEQ ID NO: 4-6; (b) upstream and downstream primers and probes for detecting candida glabrata as shown in SEQ ID NO: 1-3; (c) upstream and downstream primers and probes for detecting candida tropicalis as shown in SEQ ID NO: 7-9; and (d) an upstream primer, a downstream primer and a probe which are shown as SEQ ID NO: 10-12 and are used for detecting cryptococcus. The primer probe combination provided by the invention can be used for realizing rapid, high-sensitivity and high-specificity detection of blood flow infection pathogens.

The invention discloses a method for efficiently preparing vecubromamide reductant by using microbial whole cells, which comprises the following steps of: performing fermentation culture on carbonyl reductase-producing candida tropicalis to obtain fermentation liquor containing growth cells or wet thallus resting cells obtained by centrifuging the fermentation liquor as a catalyst, and vecubromamide diamine as a substrate; the method comprises the following steps of: forming a conversion system by taking vinyl acetate as a raw material, hydroxyethyl-beta-cyclodextrin as a cosolvent and a buffer solution with the pH value of 6-10.0 as a reaction medium, completely reacting in a shaking table at the temperature of 20-40 DEG C and the speed of 100-180r / min, and separating and purifying a reaction solution to obtain the vecuronium bromide reduction product. According to the method, no extra coenzyme needs to be added, and the microorganisms can be easily cultured on a large scale within a short time, so that a large number of whole cells are obtained to serve as the biocatalyst, the cost is low, the conversion cost is effectively reduced, and the conversion efficiency is remarkably improved.

The invention relates to the technical field of microorganisms, and discloses a candida tropicalis strain. The candida tropicalis strain is a candida tropicalis SDU2 strain, the classification name is Candida tropicalis, the preservation number is CGMCC No.34306, the preservation date is April 23, 2025, the preservation unit is China General Microbiological Culture Collection Center, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Beichen West Road, Chaoyang District, Beijing. The candida tropicalis SDU2 strain has grease production capacity and high-temperature tolerance, and can convert waste biomass into biodiesel. The microbial inoculum prepared from the candida tropicalis SDU2 strain can be synchronously saccharified and fermented with biomass, so that the problem of high cost caused by poor strain adaptability in the traditional biomass high-valued comprehensive utilization process can be solved. The invention further discloses a candida tropicalis agent as well as a preparation method and application thereof.

The invention relates to the technical field of cosmetics, and particularly discloses an anti-hair-loss composition as well as a preparation method and application thereof. The invention relates to a preparation method of an anti-hair loss composition, which comprises the following steps: cleaning and drying a fermentation substrate, crushing into slurry, sterilizing, inoculating lactobacillus plantarum, candida tropicalis, schizosaccharomyces cerevisiae and monascus purpureus strains at a proper temperature, carrying out sealed fermentation, and after fermentation is completed, carrying out centrifugal separation on the fermentation liquor to obtain the anti-hair loss composition. Taking supernate, sterilizing, and performing vacuum freeze drying to obtain the anti-alopecia composition. According to the application of the anti-hair-loss composition, the anti-hair-loss composition is added into a hair product. The anti-hair loss composition disclosed by the invention is natural in source, and can promote absorption of skin to active substances, so that the anti-hair loss composition has the effects of rapidly inhibiting scalp oil and reducing hair loss.

The invention provides a preparation method and application of alpinia oxyphylla leaf essential oil, and relates to the field of natural plant extracts. The alpinia oxyphylla leaf essential oil is applied to preparation of a bacteriostatic agent for inhibiting bacteria or / and fungi, and the bacteria comprise one or more of klebsiella pneumoniae, salmonella enterobacter and shigella flexneri. The fungi comprise one or more of candida tropicalis and malassezia furfur; the preparation method of the alpinia oxyphylla leaf essential oil comprises the following steps that alpinia oxyphylla leaves are taken, dried and smashed, the smashed alpinia oxyphylla leaves are placed in water to be soaked, the material-liquid mass ratio is 1: (15-17), and the soaking time is 0.8-1.2 h; after soaking, distillation is carried out, the distillation time is 4.5-5.5 h, and the distillation temperature is 170-200 DEG C; and after distillation is finished, collecting upper oily liquid to obtain the alpinia oxyphylla leaf essential oil. The prepared alpinia oxyphylla leaf essential oil has broad-spectrum antibacterial and bactericidal effects, and the antibacterial and bactericidal effects are good.

The invention relates to a candida tropicalis engineering bacterium, a preparation method thereof and a sewage treatment method, and belongs to the technical field of genetic engineering. According to the candida tropicalis engineering bacteria, Candida tropicalis CBW-2 delta URA3 (the preservation number is CGMCC NO.31647) is used as a chassis strain, ACS genes are knocked out, and a promoter is transferred to heterologous expression alpha-amylase genes; the promoter is an endogenous promoter. Candida tropicalis is an unconventional yeast with industrial significance, has the characteristics of high cell growth speed and high environmental tolerance, and has sewage treatment potential. The ACS gene is knocked out, so that the activity of acetyl coenzyme A synthetase is obviously reduced, and the acetic acid accumulation amount of the strain is increased. By efficiently expressing alpha-amylase, the starch component can be efficiently converted into acetic acid, acetic acid can be provided for a heterotrophic nitrification-aerobic denitrification strain as a carbon source, and an exogenous carbon source does not need to be added.

The present application relates to a kind of containing fatty acyl-coa dehydrogenase engineering bacteria and its application method, belong to microbial fermentation technical field.The engineering bacteria containing fatty acyl-coa dehydrogenase gene in the present application include fatty acyl-coa dehydrogenase gene and fatty acyl-coa thioesterase gene, the fatty acyl-coa dehydrogenase gene is derived from Candida tropicalis or Yarrowia lipolytica, its nucleotide sequence is SEQ ID NO.1 or SEQ ID NO.2;The nucleotide sequence of the fatty acyl-coa thioesterase gene is SEQ ID NO.3.Fatty acyl-coa dehydrogenase from Candida tropicalis and Yarrowia lipolytica, combined with existing fatty acyl-coa thioesterase, jointly convert 10-hydroxy decanoic acid, significantly improve the conversion rate of 10-hydroxy-2-decenoic acid, so that the industrialization production of 10-hydroxy-2-decenoic acid becomes possible.

The invention belongs to the technical field of environmental microorganisms, and provides candida tropicalis HWDY-JD-001 and an application of the candida tropicalis HWDY-JD-001 in degradation of aliphatic organic amine. The preservation number of the strain provided by the invention is CGMCC (China General Microbiological Culture Collection Center) The strain has the capability of efficiently degrading the aliphatic organic amine, is added into a sewage treatment biochemical system containing the aliphatic organic amine after being fermented and cultured, and can efficiently degrade the aliphatic organic amine by improving the degradation efficiency and removal rate of the biochemical system on the aliphatic organic amine. The toxicity and impact of the aliphatic organic amine on denitrifying microorganisms such as nitrifying bacteria and denitrifying bacteria in a biochemical system are reduced, the operation stability of the biochemical system is improved, and the method has important practical application value and environmental significance for sewage denitrification.

The application provides a water-retaining soil conditioner containing high-efficiency degrading bacteria and a preparation method and application thereof, and belongs to the technical field of soil improvement.The water-retaining soil conditioner comprises a carboxymethylated modified humic acid-chitosan compound, modified attapulgite powder, a composite microbial inoculum, konjac glucosan, polyacrylic acid, bentonite, polyglutamic acid, sodium alginate, mushroom residue, seaweed residue and straw compost; the composite microbial inoculum is obtained by combination of Candida tropicalis, Bacillus smithii and Bacillus pasteurii.The application realizes precise adaptation of the carrier and the high-efficiency degrading bacterial group by optimizing the design of the composite water-retaining carrier and introducing specific bacterial combinations, strengthens the synergistic effect of water retention, bacteria fixation, slow release and soil improvement, realizes water retention, degradation and fertilization synergistic effect, and has important practical significance and application value for improving soil quality, guaranteeing crop production and promoting the development of ecological agriculture.

The invention relates to the technical field of soil remediation, in particular to a saline-alkali soil remediation fungicide and a preparation method thereof. Comprising the following raw materials in parts by weight: 4 to 8 parts of bacillus subtilis, 8 to 15 parts of purple sulfur bacteria, 10 to 17 parts of rhizobium, 1 to 3 parts of bacillus megatherium, 1 to 3 parts of candida tropicalis, 1 to 3 parts of aspergillus niger, 0.05 to 0.2 part of proline, 0.5 to 2.5 parts of humic acid, 3 to 5 parts of modified charcoal, 0.1 to 0.5 part of glucose, 0.4 to 1.2 parts of monopotassium phosphate and 0.3 to 0.6 part of calcium chloride. The application of the soil remediation fungicide not only improves the growth environment of the crops, but also obviously improves the salt tolerance of the crops by adjusting the stress resistance mechanism of the crops. After the soil remediation fungicide is applied, the root system development of crops is more robust, and the density and length of the root system are obviously increased, so that the crops can absorb water and nutrients from the soil deeper layer, and the surface salt damage can be effectively resisted.

The invention provides a skin acne-removing and anti-inflammatory preparation and a preparation method thereof, and belongs to the technical field of cosmetics. The preparation is prepared from the following raw materials in percentage by mass: 15 to 30 percent of acne-removing and anti-inflammatory functional components, 0.5 to 20 percent of emollient, 0.5 to 15 percent of emulsifier, 1 to 25 percent of filler, 1 to 15 percent of thickener, 0.05 to 0.3 percent of preservative, 0.01 to 0.5 percent of pH regulator, 0.005 to 0.05 percent of essence and the balance of water, the acne-removing and anti-inflammatory functional components are obtained by sequentially performing enzymolysis and fermentation on traditional Chinese medicine raw materials; the traditional Chinese medicine raw materials comprise radix paeoniae rubra, seaweed, honeysuckle, dandelion and rose; a composite enzymolysis agent is used for enzymolysis, and the composite enzymolysis agent comprises beta-galactosidase, coenzyme A, laccase and maltase; a composite leavening agent is used for fermentation, and the composite leavening agent comprises xylanolyticus, saccharopolyspora spinosa, bacillus licheniformis and candida tropicalis. The preparation prepared by the invention is reasonable in formula composition, not only has excellent acne-removing and anti-inflammatory effects, but also has certain stability.

The application discloses a kind of protein feed using potato residue solid fermentation and a preparation method thereof.Mainly with potato residue as main raw material, aspergillus niger, Candida tropicalis, Candida lipolytica and Saccharomyces cerevisiae are used as collaborative fermentation strains to carry out aerobic-anaerobic fermentation, and through single factor test and orthogonal test, the production process of solid fermentation bacterial protein feed using potato residue as raw material is studied.The bacterial protein feed process is produced by adopting composite strain solid fermentation, which can significantly increase the crude protein content and true protein content in fermentation product, improve the nutritional value, improve animal growth performance, and increase the shelf life and stability of protein feed, and also provides a new mode for non-grain type feed resources, especially potato residue waste resource utilization, effectively alleviates the plight of conventional feed resource shortage.

The invention provides a method for pretreating livestock and poultry manure by adopting a composite functional microbial agent and application, and belongs to the technical field of ecological agriculture. According to the invention, pretreatment with a composite functional microbial agent (bacillus subtilis, bacillus licheniformis and candida tropicalis are compounded according to a specific ratio) is combined with a hermetia illucens larva synergistic treatment process, so that the livestock and poultry manure recycling efficiency can be remarkably improved. According to the technology, the excrement reduction effect is greatly enhanced, emission of ammonia gas and greenhouse gas is effectively inhibited, and meanwhile high-nutritive-value insect powder and high-safety insect excrement products are produced. The comprehensive efficiency of the strain is clearly superior to that of single microbial inoculum fermentation, hermetia illucens independent treatment and a commercially available microbial inoculum control group, and the strain-insect synergistic system is proved to have outstanding advantages in low-carbon conversion and high-value utilization of feces.

This invention discloses a plant essential oil composition with anti-Candida activity and its preparation method, which consists of a heavy oil component of Sichuan pepper and a light oil component of dried ginger in a volume ratio of 1:8 to 2:1. Research in this invention has found that, after specific volume ratios, particularly when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 1:8, it exhibits a synergistic effect against Candida albicans, Candida tropicalis, and Candida glabrata; when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 2:1, it exhibits a very good synergistic effect against Candida parapsilosis. This invention allows the heavy oil component of Sichuan pepper and the light oil component of dried ginger to be formulated into an anti-Candida drug in a specific volume ratio.

The present invention provides a microbial composition and its application in the preparation of food additives, belonging to the technical field of microbial fermentation. The microbial composition described in the present invention includes Bifidobacterium animalis, Enterococcus durans, and Candida tropicalis. The food additive obtained by fermenting yam, selenium-rich black beans, wolfberries, licorice, tangerine peel, and tremella fuciformis with this microbial composition has the effect of helping sleep, can be taken for a long time, and is safe, nutritious, and healthy.