Composite biological antimicrobial for preventing wilt and greensickness of cotton

A biocontrol fungicide and Fusarium wilt technology, applied in the direction of biocide, bactericide, biocide, etc., can solve the problems of no public name anti-cotton Fusarium wilt control effect, etc., to reduce the dead seedling rate and increase the yield rate The effect of improving and increasing production

Inactive Publication Date: 2011-03-30
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this document only discloses the class of antagonistic endophytic bacteria, and does not disclose the name of each bacterial strain and the control effect of resisting cotton fusarium wilt

Method used

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  • Composite biological antimicrobial for preventing wilt and greensickness of cotton
  • Composite biological antimicrobial for preventing wilt and greensickness of cotton
  • Composite biological antimicrobial for preventing wilt and greensickness of cotton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation of the composite biocontrol bacterium powder of embodiment 1 anti-cotton fusarium wilt

[0019] The composite biocontrol agent for resisting cotton fusarium wilt consists of agent A, agent B, agent C and agent D. Bacterial agent A, bacterial agent B, bacterial agent C and bacterial agent D are prepared according to the following method:

[0020] Preparation of Potato Dextrose Agar Medium (PDA): Weigh 200g of peeled potatoes, cut into pieces and boil for 30min, then filter the juice with gauze, add 20.0g of glucose and 20.0g of agar, add water to 1000mL after melting, and distribute to Ten 150mL Erlenmeyer flasks can be sterilized at 121°C for 30 minutes.

[0021] Candida tropicalis was cultivated in the above PDA medium at 28°C for 3 days with shaking at 200 r / min, and all the fermentation broth was collected and dried under low-temperature negative pressure as bacterial agent A.

[0022] Streptomyces microflavus was cultivated in the above PDA medium ...

Embodiment 2

[0026] The preparation of the composite biocontrol bacterium powder of embodiment 2 anti-cotton fusarium wilt

[0027] The compound biocontrol fungicides used for resisting cotton fusarium wilt include fungicide A, fungicide B, fungicide C and fungicide D. Wherein bacterial agent A, bacterial agent B, bacterial agent C and microbial agent D are prepared according to the following method:

[0028] Preparation of Potato Dextrose Agar Medium (PDA): Weigh 200g of peeled potatoes, cut into pieces and boil for 30min, then filter the juice with gauze, add 20.0g of glucose and 20.0g of agar, add water to 1000mL after melting, and distribute to Ten 150mL Erlenmeyer flasks can be sterilized at 121°C for 30 minutes.

[0029] Candida tropicalis was cultivated in the above PDA medium at 28°C for 3 days with shaking at 200 r / min, and all the fermentation broth was collected and dried under low-temperature negative pressure as bacterial agent A.

[0030] Streptomyces microflavus was cultiv...

Embodiment 3

[0034] The preparation of the composite biocontrol bacteria water agent of embodiment 3 anti-cotton fusarium wilt

[0035] The composite biocontrol agent for resisting cotton fusarium wilt consists of agent A, agent B, agent C and agent D. Bacterial agent A, bacterial agent B, bacterial agent C and bacterial agent D are prepared according to the following method:

[0036] Preparation of Potato Dextrose Agar Medium (PDA): Weigh 200g of peeled potatoes, cut into pieces and boil for 30min, then filter the juice with gauze, add 20.0g of glucose and 20.0g of agar, add water to 1000mL after melting, and distribute to Ten 150mL Erlenmeyer flasks can be sterilized at 121°C for 30 minutes.

[0037] Candida tropicalis was cultivated in the above PDA medium at 28° C. and shaken at 200 r / min for 3 days, and all the fermentation broth was collected as bacterial agent A.

[0038] Streptomyces microflavus was cultured in the above PDA medium at 28° C. and shaken at 200 r / min for 3 days, an...

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Abstract

The invention relates to a composite biological antimicrobial for preventing wilt and greensickness of cotton. The active components of the composite biological antimicrobial comprise Candida tropicalis, streptomyces microflavus, Trichoderma viride and Bacillus subtilis. The composite biological antimicrobial for preventing wilt and greensickness of cotton can reduce the seedling death rate effectively, has obvious preventing and treating effect on wild and greensickness of cotton and provides the cotton output.

Description

technical field [0001] The invention relates to a compound biocontrol agent for resisting cotton fusarium wilt, in particular to a compound biocontrol agent for resisting cotton fusarium wilt by improving soil microbial flora. Background technique [0002] Cotton Fusarium Wilt Disease, caused by Fusarium oxysporium f.sp. Vasinfectum, is one of the most devastating and devastating cotton diseases. When environmental conditions are favorable for the occurrence of Fusarium wilt Can lead to a substantial reduction in cotton production. In the early 1930s, due to the introduction of American cotton into various places without quarantine and seed disinfection, cotton fusarium wilt, which was introduced along with the seeds, has now spread to most cotton areas in the country and has become a serious disease problem in cotton production. The disease was first discovered in the United States in 1892, and spread rapidly with the transportation of cotton seeds. At present, it occurs i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N63/00A01P3/00
Inventor 李双来胡诚乔艳陈云峰童军
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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