51 results about "C. tropicalis" patented technology

The invention relates to the technical field of biological medicine, in particular to application of a combination of a Cd1 and Mdr1 double-target inhibitor and an azole drug in preparation of an antifungal drug. Based on the fact that the compound as shown in the formula (I) can be used as a CCd1 and Mdr1 double-target inhibitor, the inhibition effect of the compound on candida albicans, candida auricula, candida glabrata, candida tropicalis, trichophyton rubrum and trichophyton indicus when the compound is combined with azole drugs is further evaluated, and the result shows that the compound as shown in the formula (I) has no obvious antibacterial activity on various fungi, and can be used for preparing a medicine for treating various fungi. The drug resistance of CCd1 and Mdr1 overexpression drug-resistant fungi can be reversed, the activity of Candida albicans, Candida auricula, Candida glabrata, Candida tropicalis, Trichophyton rubrum and Trichophyton indicus can be synergistically inhibited when the drug is combined with azole drugs, the treatment dosage of the azole drugs is effectively reduced, and the drug toxicity is small. Therefore, the compound shown in the formula (I) can improve the antifungal effect of azole drugs and reduce the drug resistance of the azole drugs.

The invention relates to the technical field of culture media, and particularly discloses a candida chromogenic culture medium as well as a preparation method and application thereof. The candida chromogenic culture medium disclosed by the invention is prepared from the following components in concentration: 10 to 11 g / L of peptone, 12 to 17 g / L of agar, 7 to 9 g / L of glucose, 1 to 2 g / L of chromogenic substrate and 0.4 to 0.6 g / L of bacteriostatic agent, the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D-galactosamine and 4-methylumbelliferone-beta-D-N-acetyl galactosamine, and the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D- The bacteriostatic agent is prepared by mixing chloramphenicol, taurocholic acid and nalidixic acid. When being used for detecting and identifying candida albicans, candida tropicalis, candida krusei and candida glabrata, the chromogenic culture medium provided by the invention has strong specificity, short identification time and wide practical application value.

This invention discloses the application of larvicin or its pharmaceutical salt in the preparation of antifungal drugs. The structure of larvicin is shown below. This invention discovers an effective antifungal compound with pharmaceutical potential: larvicin. The compound larvicin of this invention exhibits clear antifungal activity against pathogenic fungi such as *Candida albicans*, *Candida auris*, *Candida tropicalis*, *Candida parapsilosis*, *Candida krusei*, *Cryptococcus neoformans*, *Trichophyton mentagrophytes*, and *Trichophyton rubrum*, with a minimum inhibitory concentration (MIC). 90 The range is 2–8 μg / mL.

The invention relates to the technical field of microorganisms, and discloses a candida tropicalis strain. The candida tropicalis strain is a candida tropicalis SDU2 strain, the classification name is Candida tropicalis, the preservation number is CGMCC No.34306, the preservation date is April 23, 2025, the preservation unit is China General Microbiological Culture Collection Center, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Beichen West Road, Chaoyang District, Beijing. The candida tropicalis SDU2 strain has grease production capacity and high-temperature tolerance, and can convert waste biomass into biodiesel. The microbial inoculum prepared from the candida tropicalis SDU2 strain can be synchronously saccharified and fermented with biomass, so that the problem of high cost caused by poor strain adaptability in the traditional biomass high-valued comprehensive utilization process can be solved. The invention further discloses a candida tropicalis agent as well as a preparation method and application thereof.

The invention relates to the technical field of cosmetics, and particularly discloses an anti-hair-loss composition as well as a preparation method and application thereof. The invention relates to a preparation method of an anti-hair loss composition, which comprises the following steps: cleaning and drying a fermentation substrate, crushing into slurry, sterilizing, inoculating lactobacillus plantarum, candida tropicalis, schizosaccharomyces cerevisiae and monascus purpureus strains at a proper temperature, carrying out sealed fermentation, and after fermentation is completed, carrying out centrifugal separation on the fermentation liquor to obtain the anti-hair loss composition. Taking supernate, sterilizing, and performing vacuum freeze drying to obtain the anti-alopecia composition. According to the application of the anti-hair-loss composition, the anti-hair-loss composition is added into a hair product. The anti-hair loss composition disclosed by the invention is natural in source, and can promote absorption of skin to active substances, so that the anti-hair loss composition has the effects of rapidly inhibiting scalp oil and reducing hair loss.

The present application relates to a kind of containing fatty acyl-coa dehydrogenase engineering bacteria and its application method, belong to microbial fermentation technical field.The engineering bacteria containing fatty acyl-coa dehydrogenase gene in the present application include fatty acyl-coa dehydrogenase gene and fatty acyl-coa thioesterase gene, the fatty acyl-coa dehydrogenase gene is derived from Candida tropicalis or Yarrowia lipolytica, its nucleotide sequence is SEQ ID NO.1 or SEQ ID NO.2;The nucleotide sequence of the fatty acyl-coa thioesterase gene is SEQ ID NO.3.Fatty acyl-coa dehydrogenase from Candida tropicalis and Yarrowia lipolytica, combined with existing fatty acyl-coa thioesterase, jointly convert 10-hydroxy decanoic acid, significantly improve the conversion rate of 10-hydroxy-2-decenoic acid, so that the industrialization production of 10-hydroxy-2-decenoic acid becomes possible.

This application relates to the field of biotechnology, and particularly to primer pairs, primer-probe compositions, PCR premixes, kits, and methods for detecting multiple respiratory pathogens. Nucleic acid sequences are shown in primer pairs as indicated by SEQ ID NO: 1-2, 4-5, 7-8, 10-11, 13-14, 16-17, 19-20, 22-23, 25-26, 28-29, 31-32, 34-35, 37-38, and 40-41. For target respiratory pathogens, this application designs specific amplification primer pairs, paired with suitable probes, enabling the simultaneous detection of eight targets. Furthermore, the detection process avoids interference from heme, trimethoprim, sulfamethoxazole, amphotericin B, itraconazole, fluconazole, azithromycin, adrenaline, and lidocaine hydrochloride in the test sample. It exhibits good anti-interference properties and shows no cross-reactivity with *Rhodococcus equi*, *Candida tropicalis*, and *Candida krusei*.

The invention belongs to the technical field of environmental microorganisms, and provides candida tropicalis HWDY-JD-001 and an application of the candida tropicalis HWDY-JD-001 in degradation of aliphatic organic amine. The preservation number of the strain provided by the invention is CGMCC (China General Microbiological Culture Collection Center) The strain has the capability of efficiently degrading the aliphatic organic amine, is added into a sewage treatment biochemical system containing the aliphatic organic amine after being fermented and cultured, and can efficiently degrade the aliphatic organic amine by improving the degradation efficiency and removal rate of the biochemical system on the aliphatic organic amine. The toxicity and impact of the aliphatic organic amine on denitrifying microorganisms such as nitrifying bacteria and denitrifying bacteria in a biochemical system are reduced, the operation stability of the biochemical system is improved, and the method has important practical application value and environmental significance for sewage denitrification.

The application provides a water-retaining soil conditioner containing high-efficiency degrading bacteria and a preparation method and application thereof, and belongs to the technical field of soil improvement.The water-retaining soil conditioner comprises a carboxymethylated modified humic acid-chitosan compound, modified attapulgite powder, a composite microbial inoculum, konjac glucosan, polyacrylic acid, bentonite, polyglutamic acid, sodium alginate, mushroom residue, seaweed residue and straw compost; the composite microbial inoculum is obtained by combination of Candida tropicalis, Bacillus smithii and Bacillus pasteurii.The application realizes precise adaptation of the carrier and the high-efficiency degrading bacterial group by optimizing the design of the composite water-retaining carrier and introducing specific bacterial combinations, strengthens the synergistic effect of water retention, bacteria fixation, slow release and soil improvement, realizes water retention, degradation and fertilization synergistic effect, and has important practical significance and application value for improving soil quality, guaranteeing crop production and promoting the development of ecological agriculture.

The invention relates to the technical field of soil remediation, in particular to a saline-alkali soil remediation fungicide and a preparation method thereof. Comprising the following raw materials in parts by weight: 4 to 8 parts of bacillus subtilis, 8 to 15 parts of purple sulfur bacteria, 10 to 17 parts of rhizobium, 1 to 3 parts of bacillus megatherium, 1 to 3 parts of candida tropicalis, 1 to 3 parts of aspergillus niger, 0.05 to 0.2 part of proline, 0.5 to 2.5 parts of humic acid, 3 to 5 parts of modified charcoal, 0.1 to 0.5 part of glucose, 0.4 to 1.2 parts of monopotassium phosphate and 0.3 to 0.6 part of calcium chloride. The application of the soil remediation fungicide not only improves the growth environment of the crops, but also obviously improves the salt tolerance of the crops by adjusting the stress resistance mechanism of the crops. After the soil remediation fungicide is applied, the root system development of crops is more robust, and the density and length of the root system are obviously increased, so that the crops can absorb water and nutrients from the soil deeper layer, and the surface salt damage can be effectively resisted.

The application discloses a kind of protein feed using potato residue solid fermentation and a preparation method thereof.Mainly with potato residue as main raw material, aspergillus niger, Candida tropicalis, Candida lipolytica and Saccharomyces cerevisiae are used as collaborative fermentation strains to carry out aerobic-anaerobic fermentation, and through single factor test and orthogonal test, the production process of solid fermentation bacterial protein feed using potato residue as raw material is studied.The bacterial protein feed process is produced by adopting composite strain solid fermentation, which can significantly increase the crude protein content and true protein content in fermentation product, improve the nutritional value, improve animal growth performance, and increase the shelf life and stability of protein feed, and also provides a new mode for non-grain type feed resources, especially potato residue waste resource utilization, effectively alleviates the plight of conventional feed resource shortage.

The invention provides a method for pretreating livestock and poultry manure by adopting a composite functional microbial agent and application, and belongs to the technical field of ecological agriculture. According to the invention, pretreatment with a composite functional microbial agent (bacillus subtilis, bacillus licheniformis and candida tropicalis are compounded according to a specific ratio) is combined with a hermetia illucens larva synergistic treatment process, so that the livestock and poultry manure recycling efficiency can be remarkably improved. According to the technology, the excrement reduction effect is greatly enhanced, emission of ammonia gas and greenhouse gas is effectively inhibited, and meanwhile high-nutritive-value insect powder and high-safety insect excrement products are produced. The comprehensive efficiency of the strain is clearly superior to that of single microbial inoculum fermentation, hermetia illucens independent treatment and a commercially available microbial inoculum control group, and the strain-insect synergistic system is proved to have outstanding advantages in low-carbon conversion and high-value utilization of feces.

This invention discloses a plant essential oil composition with anti-Candida activity and its preparation method, which consists of a heavy oil component of Sichuan pepper and a light oil component of dried ginger in a volume ratio of 1:8 to 2:1. Research in this invention has found that, after specific volume ratios, particularly when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 1:8, it exhibits a synergistic effect against Candida albicans, Candida tropicalis, and Candida glabrata; when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 2:1, it exhibits a very good synergistic effect against Candida parapsilosis. This invention allows the heavy oil component of Sichuan pepper and the light oil component of dried ginger to be formulated into an anti-Candida drug in a specific volume ratio.

The present application relates to the technical field of biological medicine, in particular to the application of Cdr1 and Mdr1 double-target inhibitor combined with azole drugs in the preparation of antifungal drugs. Based on the compound shown in formula (I) can be used as Cdr1 and Mdr1 double-target inhibitor, the inhibitory effect of the compound on Candida albicans, Candida auris, Candida glabrata, Candida tropicalis, Trichophyton rubrum and Trichophyton indiana combined with azole drugs was further evaluated. The results show that the compound shown in formula (I) itself has no obvious antibacterial activity on various fungi, but can reverse the drug resistance of Cdr1 and Mdr1 overexpression drug-resistant fungi. The combination of the compound with azole drugs can synergistically inhibit the activity of Candida albicans, Candida auris, Candida glabrata, Candida tropicalis, Trichophyton rubrum and Trichophyton indiana, effectively reduce the therapeutic dose of azole drugs, and the drug toxicity is small. Therefore, the compound shown in formula (I) can improve the antifungal effect of azole drugs and reduce the drug resistance of azole drugs.

The invention discloses a candida tropicalis engineering bacterium for producing 1, 2, 4-butantriol as well as a construction method and application of the candida tropicalis engineering bacterium, and belongs to the technical field of microorganisms. According to the recombinant candida tropicalis strain constructed by the invention, scaffold protein ligand-enzyme fusion protein is subjected to integrated expression through metabolic engineering transformation, and a scaffold protein structural domain GBD-SH3-PDZ is overexpressed on the basis of the scaffold protein ligand-enzyme fusion protein; a glucose repression gene MIG1 gene is knocked out; the KdcA mutant is subjected to overexpression, the yield of 1, 2, 4-butantriol can be obviously increased, the shake flask yield of 1, 2, 4-butantriol is increased to 2.1 g / L from 0.6 g / L of the original strain BTO-01, the yield is increased by 3.35 times, and the yield of the strain BTO-14 in a 5L tank reaches 11.18 g / L through amplification culture of a fermentation tank.

The present application relates to a kind of Candida tropicalis engineering bacteria and its preparation method and the method for treating sewage, belong to genetic engineering technical field.Candida tropicalis engineering bacteria with Candida tropicalis CBW-2 ΔURA3 (preservation number: CGMCC NO.31647) as chassis strain, knock out ACS gene and transfer into promoter heterologous expression alpha-amylase gene;The promoter is endogenous promoter.Candida tropicalis is a kind of unconventional yeast with industrial significance, with the characteristics of fast cell growth, strong environmental tolerance, has the potential of sewage treatment.The present application significantly reduces the activity of acetyl-coenzyme A synthetase by knocking out ACS gene, increases the acetic acid accumulation of strain.And by high-efficiency expression alpha-amylase, starch component can be efficiently converted into acetic acid, can provide acetic acid as carbon source for heterotrophic nitrification-aerobic denitrification strain, without adding exogenous carbon source.

The invention relates to the technical field of biological medicine, in particular to an antifungal pharmaceutical composition based on a (1, 3)-beta-D-glucan synthase inhibitor. In-vitro experiment results show that the antibacterial peptide LL-37 and (1, 3)-beta-D-glucan synthase inhibitors (caspofungin, micafungin, anidulafungin and iremifungin) are combined for use to achieve the effect of synergistically inhibiting candida albicans, candida auricula, candida tropicalis, candida glabrata, candida parapsilosis and candida krusei, so that the antibacterial peptide LL-37 can be combined with the (1, 3)-beta-D-glucan synthase inhibitors (caspofungin, micafungin, anidulafungin and iremifungin) to achieve the effect of inhibiting the (1, 3)-beta-D-glucan synthase inhibitors). And 3) preparing the antifungal drug by combining the 1, 2, 4, 5, 6, 7, 8, 8, 9, 10- However, the antibacterial peptide LL-37 is easily degraded by in-vivo protease, so that the expression of the antibacterial peptide LL-37 in an organism is up-regulated by adopting an antibacterial peptide LL-37 expression accelerant, and the antibacterial peptide LL-37 and a (1, 3)-beta-D-glucan synthetase inhibitor are synergistically antibacterial in the organism.

The invention provides a Candida tropicalis ERG11 gene copy number variation digital PCR (polymerase chain reaction) detection method, the total copy number of ERG11 genes is detected through a group of universal primers, a wild type probe of an A395 site of the ERG11 genes in a detection sample and a mutant type probe of the A395 site of the ERG11 genes, and the total copy number of the ERG11 genes is equal to the sum of the copy number of wild target spots and the copy number of mutant target spots; in the PCR detection method, the copy number of the wild target and the copy number of the mutation target are corrected by using the copy number of two reference genes. Compared with an ERG11 gene mutation and copy number variation detection method based on a fluorescent quantitative PCR method, the accuracy of copy number variation detection is greatly improved, and the problem of pain points of tandem sequence copy number recognition and quantitative detection in the candida tropicalis drug resistance detection process can be effectively solved.

The invention discloses a candida tropicalis engineering bacterium for producing astaxanthin, and belongs to the field of metabolic engineering. Candida tropicalis ANT-06 is used as a starting strain to integrally express a heterologous gene CrtZ and a gene CrtW, the heterologous gene CrtZ and the gene CrtW are subjected to fusion expression with a peroxisome signal peptide ePTS and a lipid droplet signal peptide HD2, and the yield of astaxanthin can be obviously increased through a combined strategy of knocking out a phosphatidic acid phosphatase gene PAH1 and a functional homolog gene FLD1 of human seipin. And after combined fermentation optimization, fermentation culture at 22 DEG C and addition of 10% of dodecane, 2.5% of DMSO and 2.5 mM of betaine, the shake flask fermentation yield of the astaxanthin is increased to 320.1 mg / L, and the amplification fermentation yield of a 5L fermentation tank is increased to 3096.2 mg / L. The preparation method has the characteristics of low cost, high efficiency, simplicity in operation, wide prospect and the like.

The invention discloses a preparation method of a healthy and environment-friendly household lawn planting fertilizer, and belongs to the technical field of agricultural fertilizers. The invention relates to a preparation method of a healthy and environment-friendly household lawn planting fertilizer. The preparation method comprises the following steps: 1) efficiently converting kitchen garbage and garden waste by adopting a complex microbial inoculant (azotobacter chroococcum, thermophilic cellulose decomposition bacteria and candida tropicalis) in a specific ratio; 2) designing an intelligent response type core-shell coating structure, and realizing accurate controlled release of nutrients through the synergistic effect of a PHBV copolymer core layer, a modified starch interface layer and a temperature-sensitive nano composite shell layer; and 3) a safe combination of a bitter agent and a botanical insecticide is innovatively used. The product has the characteristics of quick start and long-acting supply, can obviously improve the soil environment, is completely biodegradable and pollution-free, and solves the problems of single function and serious pollution of the traditional fertilizer. The preparation process ensures stable product performance by optimizing fermentation parameters and an electrospray coating technology, and is particularly suitable for ecological maintenance requirements of family lawns.

The invention discloses application of a Candida tropicalis strain (Candida tropicalis) to degradation of TNT (trinitrotoluene), and further provides a screening method of the Candida tropicalis strain (Candida tropicalis). The invention also provides an application of the TNT efficient degradation strain. According to the present invention, the Candida tropicalis strain can tolerate more than 400 ppm of TNT, can complete the efficient degradation of TNT within 10 h, can effectively solve the problems of low tolerance concentration, slow metabolism rate and the like of the existing TNT metabolism microorganisms, and is mainly used for solid TNT microbial conversion, TNT wastewater treatment and in-situ purification of TNT contaminated soil.

The invention discloses a method for improving the quality of fermented grains of Baijiu, and belongs to the technical field of Baijiu fermentation regulation. According to the invention, different strains are compounded and screened to obtain the complex microbial inoculant capable of improving the ethyl acetate inhibition and the ethyl hexanoate increase of the candida tropicalis CCTCC NO: M 20222053. On the basis, the performance of the bacillus paralicheniformis CCTCC NO: M 2024869 and the performance of the candida tropicalis CCTCC NO: M 20222053 in different proportions are further detected, and the composite microbial inoculant capable of adjusting the content of ethyl acetate and ethyl hexanoate in the fermented grain fermentation process is prepared.

The present application relates to a kind of genetically engineered bacteria and its application in preparation 10-hydroxy-2-decenoic acid, belong to biological fermentation technical field.The genetically engineered bacteria of the present application includes fatty acyl-coa oxidase gene, fatty acyl-coa thioesterase gene and CYP153AM228L-CPR BM3 Fusion enzyme gene, the fatty acyl-coa oxidase gene is derived from Candida tropicalis, its nucleotide sequence is SEQ ID NO.3.The key enzyme of biosynthesis 10-hydroxy-2-decenoic acid is supplemented, integrated in the present application, fatty acid hydroxylase, fatty acyl-coa oxidase, fatty acyl-coa thioesterase is jointly transferred into host bacteria, so that each enzyme system in engineering bacteria synergistic effect, to achieve the purpose of decanoic acid as raw material one-step synthesis 10-hydroxy-2-decenoic acid.

The invention discloses a method for preparing a soil conditioner through rapid directional fermentation and alcohol extraction of panax notoginseng residues and the soil conditioner, and belongs to the technical field of preparation of soil conditioners through fermentation of traditional Chinese medicine residues. Comprising the steps that traditional Chinese medicine residues are inoculated with strains to be fermented to prepare the soil conditioner, the traditional Chinese medicine residues are alcohol-extracted pseudo-ginseng residues, the strains comprise bacillus subtilis B257, pseudomonas monteilii PM-41, myrothecium verrucaria, pyrethrum umbellioides, bacillus licheniformis and candida tropicalis, and fermentation comprises the steps of S1 high-temperature detoxification and S2 medium-temperature humification. By designing a composite strain combination and fully considering the compatibility with alcohol-extracted pseudo-ginseng dregs, the strains are scientifically coordinated. The strains are put in batches for directional continuous fermentation in two stages, the ineffective transition period of a traditional process is eliminated, the fermentation period is shortened to about 7 days, efficient conversion of medicine residues is achieved, energy consumption is reduced, the obtained soil conditioner is high in humic acid content and excellent in fertilizer efficiency, and resource utilization of alcohol extraction medicine residues is achieved.

The present invention relates to microbiological methods and media dedicated for the non-denaturing detection, differentiation and / or identification of Candida spp. Yeasts, in particular Candida auricula, Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis and Candida krusei. The invention more specifically relates to a method for identifying a yeast of the genus Candida, optionally present in a biological sample, comprising the steps of: culturing the yeast to be identified on or in a culture medium comprising:-a nutritional ingredient capable of allowing the development and growth of a yeast of the genus Candida; and-an alpha-glucosidase inducer and, optionally, an alpha-glucosidase repressor; -an acid phosphatase inducer and a phosphatase repressor; a-N-acetyl-beta-glucosaminase inducer, and a method for producing the same; and-detecting any alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase activity expressed by said yeast; and-identifying the species to which said yeast belongs, based on the activity of alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase actually expressed by said yeast.

The application belongs to the technical field of 8-(2-hydroxybenzamido) octanoic acid sodium (SNAC) synthesis, and particularly relates to recombinant Candida tropicalis and application thereof. The preservation number of the recombinant Candida tropicalis is CCTCC NO: M 20253005. The application obtains a recombinant Candida tropicalis suitable for SNAC biosynthesis through screening and genetic modification, and introduces the recombinant Candida tropicalis into the synthesis of SNAC. In the synthesis route, the recombinant Candida tropicalis is used for biofermentation conversion of 2-hydroxy-N-octyl benzamide into 8-(2-hydroxybenzamido) octanoic acid, and the 8-(2-hydroxybenzamido) octanoic acid is salted at the same time of substrate conversion, so as to synthesize SNAC. The method provided by the application uses cheap and easily obtained raw materials, has mild and easy-to-control reaction conditions, high reaction selectivity and specificity, good biological safety, small environmental pollution, and has the potential for large-scale industrial application.

The invention belongs to the technical field of synthesis of sodium 8-(2-hydroxybenzamido) caprylate (SNAC), and particularly relates to a method for biologically synthesizing SNAC, which comprises the following steps: inoculating yeast to a fermentation medium, adding 2-hydroxy-N-octyl benzamide and a salt-forming agent, controlling the pH value of the fermentation medium to be alkaline, and carrying out biological fermentation to synthesize the SNAC, wherein the yeast is selected from one of candida tropicalis, candida maltose and yarrowia lipolytica. According to the method, a biological method and a chemical method are organically fused, respective advantages of the biological method and the chemical method are fully played, a brand new SNAC synthesis route mainly depending on microbial fermentation is innovatively developed, green synthesis of the SNAC is realized by utilizing a microbial cell factory, the synthesis route of the SNAC is greatly shortened, and the method has the advantages of cheap and easily available raw materials, lower cost, mild reaction conditions, high yield and the like. And the method has the advantages of low environmental pollution, high atom economy, green production and the like.

The present application belongs to the technical field of waste recycling, and particularly relates to a method for recycling beer lees. The method is as follows: after the beer lees are extruded and puffed, Candida tropicalis SCYA4 bacterial liquid is added for fermentation, the Candida tropicalis is preserved in the China Center for Type Culture Collection on May 23, 2024, and the preservation number is CCTCC NO: M20241049. By using the Candida tropicalis SCYA4 to ferment after the beer lees are extruded and puffed, the present application can significantly improve the digestion efficiency of animals on the nutrients in the beer lees.

The application discloses a kind of multi-element biochar-based fertilizer for preventing and treating wheat soil-borne diseases and a preparation method thereof, and belongs to the technical field of biochar-based fertilizer.The multi-element biochar-based fertilizer includes the following preparation raw materials: urea, ammonium dihydrogen phosphate, potassium sulfate, multi-element biochar-based microbial agent and attapulgite powder.The multi-element biochar-based microbial agent is prepared from biochar and multi-element microbial agent.The biochar is obtained by carbonization of sugar beet molasses yeast waste residue.The strains for preparing the multi-element microbial agent include azo pseudomonas strain, bacillus subtilis and Candida tropicalis strain.The effective viable bacterial count of the multi-element microbial agent is greater than or equal to 500 million / mL.The multi-element biochar-based fertilizer prepared by the application has the effect of preventing and treating wheat soil-borne diseases, and the prevention and treatment effects on wheat basal stem rot and wheat sharp eyespot reach more than 80%, which can reduce the use of chemical pesticides, save money and labor, and reduce environmental pollution.

The invention discloses a yeast engineering strain for synthesizing 7-dehydrocholesterol as well as a construction method and application of the yeast engineering strain, and belongs to the technical field of metabolic engineering. The invention provides a recombinant Candida tropicalis, which integrates a human-derived C 24-dehydrocholesterol reductase gene HsDHCR24, and can be used for preparing the recombinant Candida tropicalis by overexpressing all genes in a front squalene synthesis route and genes in a rear squalene synthesis route. Meanwhile, by blocking a branch metabolic pathway of ergosterol synthesis and weakening the consumption of ergosterol and by virtue of an endoplasmic reticulum engineering strategy, the yield of 7-DHC obtained by shake-flask fermentation of the recombinant strain is increased from 10.1 mg / L to 432.3 mg / L, and by virtue of culture medium optimization and fed-batch fermentation of a 5L fermentation tank, the final yield reaches 3.65 g / L, so that the yield of 7-DHC is greatly improved. While the yield is increased, a thought is provided for synthesizing other sterol products from chassis cells by the candida tropicalis.