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31 results about "C. tropicalis" patented technology

In tropical countries, C. tropicalis is one of the most common colonizer and pathogen causing human disease, especially found on human skin, in the gastrointestinal tract and also in female genitourinary tract.

Anti-hair loss composition as well as preparation method and application thereof

The invention relates to the technical field of cosmetics, and particularly discloses an anti-hair-loss composition as well as a preparation method and application thereof. The invention relates to a preparation method of an anti-hair loss composition, which comprises the following steps: cleaning and drying a fermentation substrate, crushing into slurry, sterilizing, inoculating lactobacillus plantarum, candida tropicalis, schizosaccharomyces cerevisiae and monascus purpureus strains at a proper temperature, carrying out sealed fermentation, and after fermentation is completed, carrying out centrifugal separation on the fermentation liquor to obtain the anti-hair loss composition. Taking supernate, sterilizing, and performing vacuum freeze drying to obtain the anti-alopecia composition. According to the application of the anti-hair-loss composition, the anti-hair-loss composition is added into a hair product. The anti-hair loss composition disclosed by the invention is natural in source, and can promote absorption of skin to active substances, so that the anti-hair loss composition has the effects of rapidly inhibiting scalp oil and reducing hair loss.
Owner:GUANGZHOU XIMU BIOTECHNOLOGY CO LTD

Primer pairs, primer probe compositions, PCR master mixes, kits, and methods for detecting multiple respiratory pathogens

This application relates to the field of biotechnology, and particularly to primer pairs, primer-probe compositions, PCR premixes, kits, and methods for detecting multiple respiratory pathogens. Nucleic acid sequences are shown in primer pairs as indicated by SEQ ID NO: 1-2, 4-5, 7-8, 10-11, 13-14, 16-17, 19-20, 22-23, 25-26, 28-29, 31-32, 34-35, 37-38, and 40-41. For target respiratory pathogens, this application designs specific amplification primer pairs, paired with suitable probes, enabling the simultaneous detection of eight targets. Furthermore, the detection process avoids interference from heme, trimethoprim, sulfamethoxazole, amphotericin B, itraconazole, fluconazole, azithromycin, adrenaline, and lidocaine hydrochloride in the test sample. It exhibits good anti-interference properties and shows no cross-reactivity with *Rhodococcus equi*, *Candida tropicalis*, and *Candida krusei*.
Owner:SANSURE BIOTECH INC

Candida tropicalis HWDY-JD-001 and application thereof in degradation of aliphatic organic amine

The invention belongs to the technical field of environmental microorganisms, and provides candida tropicalis HWDY-JD-001 and an application of the candida tropicalis HWDY-JD-001 in degradation of aliphatic organic amine. The preservation number of the strain provided by the invention is CGMCC (China General Microbiological Culture Collection Center) The strain has the capability of efficiently degrading the aliphatic organic amine, is added into a sewage treatment biochemical system containing the aliphatic organic amine after being fermented and cultured, and can efficiently degrade the aliphatic organic amine by improving the degradation efficiency and removal rate of the biochemical system on the aliphatic organic amine. The toxicity and impact of the aliphatic organic amine on denitrifying microorganisms such as nitrifying bacteria and denitrifying bacteria in a biochemical system are reduced, the operation stability of the biochemical system is improved, and the method has important practical application value and environmental significance for sewage denitrification.
Owner:华沃德源环境技术(济南)有限公司

A water-retaining soil conditioner containing high-efficiency degrading bacteria and a preparation method and application thereof

PendingCN122104239ABio-organic fraction processingOrganic fertilisersCandida tropicalisC. tropicalis
The application provides a water-retaining soil conditioner containing high-efficiency degrading bacteria and a preparation method and application thereof, and belongs to the technical field of soil improvement.The water-retaining soil conditioner comprises a carboxymethylated modified humic acid-chitosan compound, modified attapulgite powder, a composite microbial inoculum, konjac glucosan, polyacrylic acid, bentonite, polyglutamic acid, sodium alginate, mushroom residue, seaweed residue and straw compost; the composite microbial inoculum is obtained by combination of Candida tropicalis, Bacillus smithii and Bacillus pasteurii.The application realizes precise adaptation of the carrier and the high-efficiency degrading bacterial group by optimizing the design of the composite water-retaining carrier and introducing specific bacterial combinations, strengthens the synergistic effect of water retention, bacteria fixation, slow release and soil improvement, realizes water retention, degradation and fertilization synergistic effect, and has important practical significance and application value for improving soil quality, guaranteeing crop production and promoting the development of ecological agriculture.
Owner:河南远东生物工程有限公司

Protein feed prepared by solid state fermentation using potato pulp and its preparation method

The application discloses a kind of protein feed using potato residue solid fermentation and a preparation method thereof.Mainly with potato residue as main raw material, aspergillus niger, Candida tropicalis, Candida lipolytica and Saccharomyces cerevisiae are used as collaborative fermentation strains to carry out aerobic-anaerobic fermentation, and through single factor test and orthogonal test, the production process of solid fermentation bacterial protein feed using potato residue as raw material is studied.The bacterial protein feed process is produced by adopting composite strain solid fermentation, which can significantly increase the crude protein content and true protein content in fermentation product, improve the nutritional value, improve animal growth performance, and increase the shelf life and stability of protein feed, and also provides a new mode for non-grain type feed resources, especially potato residue waste resource utilization, effectively alleviates the plight of conventional feed resource shortage.
Owner:YUXI KUAIDADUO ANIMAL HUSBANDRY TECH +2

A plant essential oil composition having anti-candida activity and a preparation method and application thereof

This invention discloses a plant essential oil composition with anti-Candida activity and its preparation method, which consists of a heavy oil component of Sichuan pepper and a light oil component of dried ginger in a volume ratio of 1:8 to 2:1. Research in this invention has found that, after specific volume ratios, particularly when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 1:8, it exhibits a synergistic effect against Candida albicans, Candida tropicalis, and Candida glabrata; when the heavy oil component of Sichuan pepper or the light oil component of dried ginger is in a volume ratio of 2:1, it exhibits a very good synergistic effect against Candida parapsilosis. This invention allows the heavy oil component of Sichuan pepper and the light oil component of dried ginger to be formulated into an anti-Candida drug in a specific volume ratio.
Owner:NINGXIA MEDICAL UNIV

Use of a dual-target inhibitor of cdr1 and mdr1 in combination with an azole in the preparation of an antifungal drug

ActiveCN120815082BOrganic active ingredientsAntimycoticsAntifungal drugCandida tropicalis
The present application relates to the technical field of biological medicine, in particular to the application of Cdr1 and Mdr1 double-target inhibitor combined with azole drugs in the preparation of antifungal drugs. Based on the compound shown in formula (I) can be used as Cdr1 and Mdr1 double-target inhibitor, the inhibitory effect of the compound on Candida albicans, Candida auris, Candida glabrata, Candida tropicalis, Trichophyton rubrum and Trichophyton indiana combined with azole drugs was further evaluated. The results show that the compound shown in formula (I) itself has no obvious antibacterial activity on various fungi, but can reverse the drug resistance of Cdr1 and Mdr1 overexpression drug-resistant fungi. The combination of the compound with azole drugs can synergistically inhibit the activity of Candida albicans, Candida auris, Candida glabrata, Candida tropicalis, Trichophyton rubrum and Trichophyton indiana, effectively reduce the therapeutic dose of azole drugs, and the drug toxicity is small. Therefore, the compound shown in formula (I) can improve the antifungal effect of azole drugs and reduce the drug resistance of azole drugs.
Owner:SHANDONG UNIV

Candida tropicalis engineering bacterium for producing 1, 2, 4-butantriol as well as construction method and application of candida tropicalis engineering bacterium

PendingCN121518295AFungiAntibody mimetics/scaffoldsGlucose repressionCandida tropicalis
The invention discloses a candida tropicalis engineering bacterium for producing 1, 2, 4-butantriol as well as a construction method and application of the candida tropicalis engineering bacterium, and belongs to the technical field of microorganisms. According to the recombinant candida tropicalis strain constructed by the invention, scaffold protein ligand-enzyme fusion protein is subjected to integrated expression through metabolic engineering transformation, and a scaffold protein structural domain GBD-SH3-PDZ is overexpressed on the basis of the scaffold protein ligand-enzyme fusion protein; a glucose repression gene MIG1 gene is knocked out; the KdcA mutant is subjected to overexpression, the yield of 1, 2, 4-butantriol can be obviously increased, the shake flask yield of 1, 2, 4-butantriol is increased to 2.1 g / L from 0.6 g / L of the original strain BTO-01, the yield is increased by 3.35 times, and the yield of the strain BTO-14 in a 5L tank reaches 11.18 g / L through amplification culture of a fermentation tank.
Owner:JIANGNAN UNIV

Candida tropicalis engineering bacteria, preparation method thereof and method for treating sewage

The present application relates to a kind of Candida tropicalis engineering bacteria and its preparation method and the method for treating sewage, belong to genetic engineering technical field.Candida tropicalis engineering bacteria with Candida tropicalis CBW-2 ΔURA3 (preservation number: CGMCC NO.31647) as chassis strain, knock out ACS gene and transfer into promoter heterologous expression alpha-amylase gene;The promoter is endogenous promoter.Candida tropicalis is a kind of unconventional yeast with industrial significance, with the characteristics of fast cell growth, strong environmental tolerance, has the potential of sewage treatment.The present application significantly reduces the activity of acetyl-coenzyme A synthetase by knocking out ACS gene, increases the acetic acid accumulation of strain.And by high-efficiency expression alpha-amylase, starch component can be efficiently converted into acetic acid, can provide acetic acid as carbon source for heterotrophic nitrification-aerobic denitrification strain, without adding exogenous carbon source.
Owner:SHANDONG UNIV

Digital PCR (Polymerase Chain Reaction) detection method and kit for copy number variation of candida tropicalis ERG11 gene

The invention provides a Candida tropicalis ERG11 gene copy number variation digital PCR (polymerase chain reaction) detection method, the total copy number of ERG11 genes is detected through a group of universal primers, a wild type probe of an A395 site of the ERG11 genes in a detection sample and a mutant type probe of the A395 site of the ERG11 genes, and the total copy number of the ERG11 genes is equal to the sum of the copy number of wild target spots and the copy number of mutant target spots; in the PCR detection method, the copy number of the wild target and the copy number of the mutation target are corrected by using the copy number of two reference genes. Compared with an ERG11 gene mutation and copy number variation detection method based on a fluorescent quantitative PCR method, the accuracy of copy number variation detection is greatly improved, and the problem of pain points of tandem sequence copy number recognition and quantitative detection in the candida tropicalis drug resistance detection process can be effectively solved.
Owner:TARGETINGONE TECH (BEIJING) CORP +1

Candida tropicalis engineering bacterium for producing astaxanthin

The invention discloses a candida tropicalis engineering bacterium for producing astaxanthin, and belongs to the field of metabolic engineering. Candida tropicalis ANT-06 is used as a starting strain to integrally express a heterologous gene CrtZ and a gene CrtW, the heterologous gene CrtZ and the gene CrtW are subjected to fusion expression with a peroxisome signal peptide ePTS and a lipid droplet signal peptide HD2, and the yield of astaxanthin can be obviously increased through a combined strategy of knocking out a phosphatidic acid phosphatase gene PAH1 and a functional homolog gene FLD1 of human seipin. And after combined fermentation optimization, fermentation culture at 22 DEG C and addition of 10% of dodecane, 2.5% of DMSO and 2.5 mM of betaine, the shake flask fermentation yield of the astaxanthin is increased to 320.1 mg / L, and the amplification fermentation yield of a 5L fermentation tank is increased to 3096.2 mg / L. The preparation method has the characteristics of low cost, high efficiency, simplicity in operation, wide prospect and the like.
Owner:JIANGNAN UNIV

Application of candida tropicalis for degrading TNT (trinitrotoluene)

The invention discloses application of a Candida tropicalis strain (Candida tropicalis) to degradation of TNT (trinitrotoluene), and further provides a screening method of the Candida tropicalis strain (Candida tropicalis). The invention also provides an application of the TNT efficient degradation strain. According to the present invention, the Candida tropicalis strain can tolerate more than 400 ppm of TNT, can complete the efficient degradation of TNT within 10 h, can effectively solve the problems of low tolerance concentration, slow metabolism rate and the like of the existing TNT metabolism microorganisms, and is mainly used for solid TNT microbial conversion, TNT wastewater treatment and in-situ purification of TNT contaminated soil.
Owner:XIAN MODERN CHEM RES INST

Method for improving quality of fermented grains of Baijiu

The invention discloses a method for improving the quality of fermented grains of Baijiu, and belongs to the technical field of Baijiu fermentation regulation. According to the invention, different strains are compounded and screened to obtain the complex microbial inoculant capable of improving the ethyl acetate inhibition and the ethyl hexanoate increase of the candida tropicalis CCTCC NO: M 20222053. On the basis, the performance of the bacillus paralicheniformis CCTCC NO: M 2024869 and the performance of the candida tropicalis CCTCC NO: M 20222053 in different proportions are further detected, and the composite microbial inoculant capable of adjusting the content of ethyl acetate and ethyl hexanoate in the fermented grain fermentation process is prepared.
Owner:JIANGNAN UNIV +1

Method for preparing soil amendment by rapidly and directionally fermenting and alcohol-extracting pseudo-ginseng dregs and soil amendment

PendingCN121800590AFungiBacteriaBacillus licheniformisCandida tropicalis
The invention discloses a method for preparing a soil conditioner through rapid directional fermentation and alcohol extraction of panax notoginseng residues and the soil conditioner, and belongs to the technical field of preparation of soil conditioners through fermentation of traditional Chinese medicine residues. Comprising the steps that traditional Chinese medicine residues are inoculated with strains to be fermented to prepare the soil conditioner, the traditional Chinese medicine residues are alcohol-extracted pseudo-ginseng residues, the strains comprise bacillus subtilis B257, pseudomonas monteilii PM-41, myrothecium verrucaria, pyrethrum umbellioides, bacillus licheniformis and candida tropicalis, and fermentation comprises the steps of S1 high-temperature detoxification and S2 medium-temperature humification. By designing a composite strain combination and fully considering the compatibility with alcohol-extracted pseudo-ginseng dregs, the strains are scientifically coordinated. The strains are put in batches for directional continuous fermentation in two stages, the ineffective transition period of a traditional process is eliminated, the fermentation period is shortened to about 7 days, efficient conversion of medicine residues is achieved, energy consumption is reduced, the obtained soil conditioner is high in humic acid content and excellent in fertilizer efficiency, and resource utilization of alcohol extraction medicine residues is achieved.
Owner:成都沃壤千里科技有限公司

Detection, differentiation and identification of yeast of genus Candida, microbiological methods and tools therefor

PendingCN122029289AMicrobiological testing/measurementCandida tropicalisCandida auris
The present invention relates to microbiological methods and media dedicated for the non-denaturing detection, differentiation and / or identification of Candida spp. Yeasts, in particular Candida auricula, Candida albicans, Candida glabrata, Candida tropicalis, Candida parapsilosis and Candida krusei. The invention more specifically relates to a method for identifying a yeast of the genus Candida, optionally present in a biological sample, comprising the steps of: culturing the yeast to be identified on or in a culture medium comprising:-a nutritional ingredient capable of allowing the development and growth of a yeast of the genus Candida; and-an alpha-glucosidase inducer and, optionally, an alpha-glucosidase repressor; -an acid phosphatase inducer and a phosphatase repressor; a-N-acetyl-beta-glucosaminase inducer, and a method for producing the same; and-detecting any alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase activity expressed by said yeast; and-identifying the species to which said yeast belongs, based on the activity of alpha-glucosidase, acid phosphatase and / or N-acetyl-beta-glucosaminase actually expressed by said yeast.
Owner:BIOMERIEUX SA

Method for biosynthesizing 8-(2-hydroxybenzamido) sodium caprylate

ActiveCN121950953AShort synthetic routeSynthetic routes are cheap and easily availableOrganic compound preparationMicroorganism based processesBiotechnologyOctanoic Acids
The invention belongs to the technical field of synthesis of sodium 8-(2-hydroxybenzamido) caprylate (SNAC), and particularly relates to a method for biologically synthesizing SNAC, which comprises the following steps: inoculating yeast to a fermentation medium, adding 2-hydroxy-N-octyl benzamide and a salt-forming agent, controlling the pH value of the fermentation medium to be alkaline, and carrying out biological fermentation to synthesize the SNAC, wherein the yeast is selected from one of candida tropicalis, candida maltose and yarrowia lipolytica. According to the method, a biological method and a chemical method are organically fused, respective advantages of the biological method and the chemical method are fully played, a brand new SNAC synthesis route mainly depending on microbial fermentation is innovatively developed, green synthesis of the SNAC is realized by utilizing a microbial cell factory, the synthesis route of the SNAC is greatly shortened, and the method has the advantages of cheap and easily available raw materials, lower cost, mild reaction conditions, high yield and the like. And the method has the advantages of low environmental pollution, high atom economy, green production and the like.
Owner:HUBEI UNIV

Method for recycling of beer spent grain

ActiveCN118581159BBiotechnologyLees
The present application belongs to the technical field of waste recycling, and particularly relates to a method for recycling beer lees. The method is as follows: after the beer lees are extruded and puffed, Candida tropicalis SCYA4 bacterial liquid is added for fermentation, the Candida tropicalis is preserved in the China Center for Type Culture Collection on May 23, 2024, and the preservation number is CCTCC NO: M20241049. By using the Candida tropicalis SCYA4 to ferment after the beer lees are extruded and puffed, the present application can significantly improve the digestion efficiency of animals on the nutrients in the beer lees.
Owner:SICHUAN AGRI UNIV

A multi-element biochar-based fertilizer for preventing and treating soil-borne diseases of wheat and a preparation method thereof

The application discloses a kind of multi-element biochar-based fertilizer for preventing and treating wheat soil-borne diseases and a preparation method thereof, and belongs to the technical field of biochar-based fertilizer.The multi-element biochar-based fertilizer includes the following preparation raw materials: urea, ammonium dihydrogen phosphate, potassium sulfate, multi-element biochar-based microbial agent and attapulgite powder.The multi-element biochar-based microbial agent is prepared from biochar and multi-element microbial agent.The biochar is obtained by carbonization of sugar beet molasses yeast waste residue.The strains for preparing the multi-element microbial agent include azo pseudomonas strain, bacillus subtilis and Candida tropicalis strain.The effective viable bacterial count of the multi-element microbial agent is greater than or equal to 500 million / mL.The multi-element biochar-based fertilizer prepared by the application has the effect of preventing and treating wheat soil-borne diseases, and the prevention and treatment effects on wheat basal stem rot and wheat sharp eyespot reach more than 80%, which can reduce the use of chemical pesticides, save money and labor, and reduce environmental pollution.
Owner:INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI

Yeast engineering strain for synthesizing 7-dehydrocholesterol as well as construction method and application of yeast engineering strain

The invention discloses a yeast engineering strain for synthesizing 7-dehydrocholesterol as well as a construction method and application of the yeast engineering strain, and belongs to the technical field of metabolic engineering. The invention provides a recombinant Candida tropicalis, which integrates a human-derived C 24-dehydrocholesterol reductase gene HsDHCR24, and can be used for preparing the recombinant Candida tropicalis by overexpressing all genes in a front squalene synthesis route and genes in a rear squalene synthesis route. Meanwhile, by blocking a branch metabolic pathway of ergosterol synthesis and weakening the consumption of ergosterol and by virtue of an endoplasmic reticulum engineering strategy, the yield of 7-DHC obtained by shake-flask fermentation of the recombinant strain is increased from 10.1 mg / L to 432.3 mg / L, and by virtue of culture medium optimization and fed-batch fermentation of a 5L fermentation tank, the final yield reaches 3.65 g / L, so that the yield of 7-DHC is greatly improved. While the yield is increased, a thought is provided for synthesizing other sterol products from chassis cells by the candida tropicalis.
Owner:JIANGNAN UNIV

Primer probe group and kit for multiplex fluorescent PCR (Polymerase Chain Reaction) and / or multiplex quantitative digital PCR detection of five candida

The invention belongs to the technical field of in-vitro molecular detection of pathogenic microorganisms, and particularly provides a primer probe group and a reagent for multiple fluorescent PCR (Polymerase Chain Reaction) and / or multiple quantitative digital PCR detection of five candida. The primer probe group comprises specific primer pairs and corresponding probes aiming at candida albicans, candida tropicalis, candida glabrata, candida parapsilosis and candida krusei. Based on the unified combination, two compatible detection paths are established: five-target synchronous qualitative detection can be realized by adopting a multiple fluorescent PCR (Polymerase Chain Reaction) method, and five-target absolute quantitative analysis can also be realized by adopting a multiple quantitative digital PCR method; the two methods can be applied independently or jointly. Through verification, the primer probe group has high specificity; the sensitivity is excellent, and DNA extracted from a clinical sample can be directly detected. The method is simple in detection process and short in time consumption, and an efficient and reliable molecular detection solution is provided for early differential diagnosis and precise medication of clinical invasive candidiasis.
Owner:HANGZHOU DILAN BIOTECHNOLOGY CO LTD

Candida tropicalis AX1 and application thereof

PendingCN121362651AFungiContaminated soil reclamationSoil remediationCandida tropicalis
The invention discloses Candida tropicalis AX1 and application thereof, the preservation number of the Candida tropicalis AX1 is CCTCC (China Center for Type Culture Collection) NO: M20251761, and the Candida tropicalis AX1 is preserved in China Center for Type Culture Collection on August 4, 2025. The strain is separated from southern humid and hot soil, shows good environmental adaptability, can grow in a temperature range of 24-36 DEG C, has an optimal temperature of 30 DEG C, has an atrazine degradation rate of 20.78% at a high temperature of 36 DEG C, and is suitable for soil remediation application in southern high-temperature and high-humidity areas.
Owner:HUAQIAO UNIVERSITY

Recombinant candida tropicalis and application thereof

The invention belongs to the technical field of synthesis of sodium 8-(2-hydroxybenzamido) caprylate (SNAC), and particularly relates to recombinant candida tropicalis and application thereof. The preservation number of the recombinant candida tropicalis is CCTCC (China Center For Type Culture Collection) NO: M 20253005. The recombinant candida tropicalis suitable for SNAC biosynthesis is obtained through screening and genetic modification and is introduced into SNAC synthesis, 2-hydroxy-N-octyl benzamide is converted into 8-(2-hydroxybenzamido) octanoic acid through biological fermentation of the recombinant candida tropicalis in the synthesis route, and the 8-(2-hydroxybenzamido) octanoic acid is converted into 8-(2-hydroxybenzamido) octanoic acid. And salifying the 8-(2-hydroxybenzamido) octanoic acid while the substrate is converted, so as to synthesize the SNAC. The method provided by the invention adopts cheap and easily available raw materials, is mild in reaction condition, easy to control, high in reaction selectivity and specificity, good in biological safety and small in environmental pollution, and has the potential of large-scale industrial application.
Owner:HUBEI UNIV

Primer pair, primer probe composition, PCR premix, kit and method for detecting multiple respiratory pathogens

ActiveCN121380460AMicrobiological testing/measurementMicroorganism based processesFluconazoleCandida tropicalis
The invention relates to the technical field of biology, in particular to a primer pair, a primer probe composition, a PCR premix, a kit and a method for detecting multiple respiratory pathogens. The invention discloses a primer pair with nucleic acid sequences as shown in SEQ ID NO: 1-2, 4-5, 7-8, 10-11, 13-14, 16-17, 19-20, 22-23, 25-26, 28-29, 31-32, 34-35, 37-38 and 40-41. Aiming at a target respiratory pathogen, a specific amplification primer pair is designed and is matched with a proper probe, so that eight-target joint detection can be realized, and the influence of heme, trimethoprim, sulfamethoxazole, amphotericin B, itraconazole, fluconazole, azithromycin, epinephrine and lidocaine hydrochloride in a detection sample can be avoided in the detection process; the anti-interference performance is good, and cross reaction with rhodococcus equi, candida tropicalis and candida krusei does not exist.
Owner:SANSURE BIOTECH INC

Fermented feed for promoting ruminants to efficiently utilize soluble protein as well as preparation method and application of fermented feed

PendingCN121926297ALow utilization efficiencyreduce pollutionFungiBacteriaCandida tropicalisC. tropicalis
The invention discloses a fermented feed for promoting ruminants to efficiently utilize soluble proteins as well as a preparation method and application of the fermented feed, and belongs to the technical field of animal feeds. The fermented feed is prepared by fermenting a substrate consisting of cottonseed meal, corn flour and bran with a compound bacteria solution, and the substrate comprises the following components in percentage by mass: 5-31% of cottonseed meal, 2-31% of corn flour and 38-93% of bran; the mass of the composite bacterial liquid is 8-10% of that of the substrate, and the composite bacterial liquid is prepared by compounding a candida tropicalis bacterial liquid and an enterococcus faecalis bacterial liquid according to a volume ratio of 1: 1, wherein the concentrations of the candida tropicalis bacterial liquid and the enterococcus faecalis bacterial liquid are both 108 CFU / mL. The preparation method comprises the following steps: crushing and sieving the substrate, mixing, adjusting the water content to 40%, spraying the compound bacteria liquid, uniformly mixing, and fermenting at a constant temperature of 30 DEG C. According to the invention, the substrate ratio and the fermentation process are optimized, the utilization efficiency of soluble protein by ruminants is improved, the rumen fermentation environment is improved, meanwhile, the nitrogen emission is reduced, and economic benefits and environmental protection values are achieved in animal production.
Owner:SHIHEZI UNIVERSITY

Zanthoxylum bungeanum maxim heavy oil component with antibacterial activity and application thereof

The application discloses a heavy Zanthoxylum bungeanum essential oil component with antibacterial activity and a preparation method thereof. The Zanthoxylum bungeanum essential oil is deeply researched, and the Zanthoxylum bungeanum essential oil is separated into a light Zanthoxylum bungeanum essential oil component and the heavy Zanthoxylum bungeanum essential oil component through a molecular distillation instrument. The antibacterial and antifungal experimental results show that the heavy Zanthoxylum bungeanum essential oil component obtained through distillation refining has better bactericidal activity on Klebsiella pneumoniae, methicillin-resistant Staphylococcus aureus, Staphylococcus aureus and corynebacterium striatum compared with the Zanthoxylum bungeanum essential oil and the light Zanthoxylum bungeanum essential oil component, and has better bactericidal activity on Candida tropicalis, Candida krusei, Candida glabrata and white Candida, and a good technical effect is achieved.
Owner:NINGXIA MEDICAL UNIV

Candida tropicalis HTZ with high yield of sophorolipids and application thereof

PendingCN122357301ABiotechnologyMicrobial enhanced oil recovery
This invention discloses a high-sophorolipid-producing Candida albicans HTZ strain, with accession number CGMCC No. 30110, which was deposited at the China General Microbiological Culture Collection Center on March 25, 2024. The high-sophorolipid-producing Candida albicans HTZ strain and its application disclosed in this invention have the following beneficial effects: (1) When the fermentation broth of this high-sophorolipid-producing Candida albicans HTZ strain is applied to microbial single-well huff and puff in shallow extra-heavy oil in the Junggar Basin, the average single-well oil increase is greater than 300 tons, and the effective period is more than 300 days; (2) When the fermentation broth of this high-sophorolipid-producing Candida albicans HTZ strain is applied to microbial enhanced oil recovery, the average daily oil increase in the well group is more than 10 tons.
Owner:CHINA PETROLEUM & CHEMICAL CORP +1

Method for synergistically promoting cotton stalk aerobic composting through cotton stalk aerobic composting biological promoter and bacterial enzyme

The invention belongs to the technical field of resources and environments, and particularly relates to a method for synergistically promoting cotton stalk aerobic composting by a cotton stalk aerobic composting biological promoter and a bacterial enzyme. The cotton stalk aerobic composting biological accelerant provided by the invention comprises an independent compound microbial agent and an independent compound enzyme. Aspergillus niger has strong capability of decomposing phenol structures of gossypol, candida tropicalis has strong capability of decomposing side chain structures of gossypol, and trichoderma viride has strong capability of decomposing fatty acid chains of gossypol. The compound microbial agent is inoculated in the initial period of aerobic composting organisms of cotton stalks, so that the microbial decomposition and degradation capacity of aerobic composting can reach the highest efficiency, the high-temperature period of composting is prolonged, the phytotoxicity of gossypol is reduced, the humification process is promoted, the microbial community structure is optimized, and the composting quality and efficiency are improved. The compound enzyme is inoculated in the decomposition period of cotton stalk aerobic composting organisms, so that the humification process can be promoted, the content of effective components such as humic acid is increased, and the utilization efficiency and value of cotton stalks are improved.
Owner:INSTITUTE OF ENVIRONMENT AND SUSTAINABLE DEVELOPMENT IN AGRICULTURE CAAS

Soil remediation agent for petroleum contaminated soil and preparation method of soil remediation agent

PendingCN121975527AOptimize ventilation structureReduce dry specific gravityFungiBacteriaCandida tropicalisBioremediation
The invention relates to the technical field of soil remediation, in particular to a soil remediation agent for petroleum-contaminated soil and a preparation method of the soil remediation agent, and aims to solve the problem that existing petroleum-contaminated soil is difficult to naturally remedy. According to the preparation method, the polluted soil is repaired by utilizing a bioremediation method, the advantages of high efficiency, good effect and small environmental damage are achieved by adding florae into the polluted soil to remove petroleum hydrocarbon pollutants, the candida tropicalis and the streptococcus faecalis are adopted for composite treatment, the candida tropicalis has the advantages of high permeability resistance, acid resistance and high metabolism efficiency of fungi, and the streptococcus faecalis and the candida tropicalis have the advantages of being high in metabolism efficiency. Streptococcus faecalis can be degraded by the petroleum hydrocarbon, pseudomycelium and spores can be generated, an extended network structure is formed in soil and can be used as a channel for bacterial migration, bacterial migration is facilitated, the treatment effect on pollutants is improved, streptococcus faecalis can grow in the environment with the petroleum hydrocarbon, the degradation effect on the petroleum hydrocarbon is good, and the degradation effect on the petroleum hydrocarbon is good. By compounding the two components, a good biodegradation effect can be achieved.
Owner:JIANGXI RUNYAO BIOLOGICAL TECH CO LTD

Preparation method of wet garbage compost deodorant

PendingCN121318576AFungiBacteriaBiotechnologyCandida tropicalis
The invention relates to the technical field of deodorant preparation, and particularly discloses a preparation method of a wet garbage compost deodorant. The deodorant comprises a composite flora consisting of bacillus subtilis, candida tropicalis, paracoccus denitrificans, bacillus methylotrophicus and agrobacterium oxydans, and is combined with a trehalose-glycerol-nano zinc oxide-L-cysteine hydrochloride freeze-drying protection system and a poly dimethyl diallyl ammonium chloride modified diatomite carrier. The preparation method comprises the following steps: separating a target strain from a wet garbage rotten substance, performing shake culture by a rejuvenation culture medium, inoculating to a seed culture medium, and performing aerated amplification; inoculating the compound seed solution into the solution A and the solution B step by step; mixing the fermentation liquor, adding a freeze-drying protective agent, and performing stepped cooling and freeze-drying to obtain bacterial powder; and finally, adding humic acid-nano titanium dioxide-beta-cyclodextrin, and loading on the modified carrier. The deodorant disclosed by the invention can be used for removing composite malodorous gas generated in a wet garbage composting process, and has the advantage of multi-strain synergistic degradation.
Owner:SHANGHAI YEMA ENVIRONMENTAL PROTECTION EQUIP ENG