62results about How to "High protein content" patented technology

The invention belongs to the technical field of biology, and particularly relates to kluyveromyces marxianus with high beta-glucan content and application of the kluyveromyces marxianus. Specifically, the invention provides a Kluyveromyces marxianus, the classification name of the Kluyveromyces marxianus is Kluyveromyces marxianus CJA0020, and the preservation number of the Kluyveromyces marxianus is CCTCC (China Center For Type Culture Collection) No.M 20241673. According to the Kluyveromyces marxianus screened by the invention, the strain growth speed is high, a strain fermentation product has higher biomass and beta-glucan content, and the beta-glucan content can reach 1.23 + / -0.06 g / L; the biomass can reach 400 g / Kg (wet weight) and the protein content can reach 45% when the strain is cultured for 32 hours during fed-batch fermentation, and the strain can also be used for producing yeast single-cell protein.

The invention relates to a method for enhancing chlorella protein production through heterotrophic fermentation. The method comprises a cell growth stage and a protein accumulation stage, in the cell growth stage, a culture medium with low-concentration ammonium nitrogen (high C / N ratio culture medium) as a nitrogen source is used for fed-batch culture, so that the chlorella cells reach high density; according to the method, protein accumulation is changed into high-concentration ammonium nitrogen (a low C / N ratio culture medium) which is used as a nitrogen source for fed-batch culture so as to promote accumulation of protein in cells, and an acetate solution is fed at the same time during the period so as to resist cytotoxicity of high-concentration ammonium to chlorella under a low C / N ratio induction culture condition; the efficient accumulation of protein in cells of the cell algae is promoted; meanwhile, the cell biomass is maintained not to be reduced or reduced slightly or even continuously increased, and finally, the technical effect of improving the quality (protein content) of a chlorella protein product and chlorella is achieved. The method is beneficial to reducing the production cost of the chlorella protein and improving the industrial economy.

The invention discloses application of lactobacillus casei or a combined microbial agent thereof to preparation of polypeptide through fermentation. The application comprises the step of preparing the polypeptide through combined fermentation of lactobacillus casei GYX-1 and lactobacillus delbrueckii subsp. Bulgaricus GYX-2. The method specifically comprises the following steps: diluting fermentation liquor obtained by fermenting in a carya illinoensis cake meal fermentation culture medium, and then performing ultrafiltration. The obtained hypoglycemic peptide does not need to be subjected to liquid chromatography separation, the amino acid sequence of the hypoglycemic peptide is directly analyzed by LC-MS / MS, the hypoglycemic peptide is synthesized to further verify the alpha-glucosidase inhibitory activity of the hypoglycemic peptide, the sequence of the hypoglycemic peptide is determined, and the efficiency is improved; the hypoglycemic peptide derived from the carya illinoensis cake meal can be used for preparing a functional preparation, and has hypoglycemic activity by inhibiting alpha-glucosidase.

The invention provides a sow feed added with puffed rice and a preparation method of the sow feed. The sow feed comprises the following components in parts by weight: 30-50 parts of puffed rice, 10-20 parts of soybean meal, 10-20 parts of wheat bran, 2-6 parts of sugar / beet pulp polypeptide, 20-30 parts of mixed forage powder, 2-6 parts of pea fibers, 2-6 parts of pennisetum purpureum dietary fibers, 5-15 parts of manioc waste, 4-10 parts of a haematococcus extract, 2-8 parts of a cercis chinensis extract, 1-4 parts of a composite strain, 0.1-0.6 part of temperature-resistant phytase and 0.5-0.9 part of myristic acid. According to the present invention, the production method is simple, and the prepared sow feed can improve the lactation yield of sows, increase the number of immune globulins in milk, improve the immunity of piglets, improve the intestinal microbiological structure and the intestinal morphology of the piglets, reduce the diarrhea of the piglets, improve the immunity of the piglets, improve the immunity of the piglets, improve the immunity of the piglets, and improve the immunity of the piglets. The growth and development of piglets are promoted.

The invention provides a methylobacterium strain JC03 for producing single-cell protein and application of the methylobacterium strain JC03 in the field of aquaculture, and belongs to the technical field of microorganisms. The methylobacterium strain JC03 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32616. The strain is high in growth efficiency, high in thallus crude protein content, rich in amino acid spectrum, high in essential amino acid content and rich in functional components for improving the immunocompetence of aquatic animals; the single-cell protein produced by the strain can completely or partially replace fish meal protein in feeds of marine fishes such as turbot, pseudosciaena crocea, grouper and rainbow trout; vegetable protein in feed of freshwater fish such as grass carp can be completely replaced; animal and plant proteins in feeds for shrimps and crabs, such as penaeus vannamei, can be completely replaced, and animal and plant proteins in stichopus japonicus feeds can be completely replaced; the immunocompetence of fed aquatic animals can be obviously improved. The invention provides a microbial strain for efficiently converting methanol to produce single-cell protein, and the microbial strain has a wide application prospect in the field of aquaculture.

The utility model discloses a corn germ meal slurry adding and drying system which is characterized in that germ meal in a leaching workshop enters a vibrating screen, an oversize outlet is connected with a lower end inlet of a first elevator, and an undersize outlet is connected with a germ meal packaging unit through a small particle discharging conveyor; an outlet in the tail end of the small particle discharging conveyor is also connected with an inlet in the lower end of the first elevator, the upper end of the first elevator is connected with an inlet of the surge bin, an outlet of the surge bin and the corn steep liquor pipe are both connected with the slurry adding mixer, and a discharging port of the slurry adding mixer is connected with the standing bed; a discharging port of the standing bed is connected with an inlet of the belt conveyor through the discharging machine, an outlet of the belt conveyor is connected with the roller dryer, an outlet of the roller dryer is connected with an inlet of the tube bundle dryer, an outlet of the tube bundle dryer is connected with the cooler through the second elevator, and a discharging port of the cooler is connected with an inlet of the smashing machine. And an outlet of the crusher is connected with the pulp adding and packaging unit. The system can improve the added value of products and increase the profit of enterprises.

The invention belongs to the technical field of medical technology and deep processing of vegetable protein polypeptides, and discloses golden chrysanthemum oligopeptide with blood fat reducing and weight losing effects, which has pancreatic lipase inhibition activity and cellular level blood fat reducing activity. The oligopeptide has pancreatic lipase inhibitory activity, and also has an inhibitory effect on fatty acid-induced accumulation of HepG2 cell lipid content. A plurality of polypeptide sequences are identified in the golden chrysanthemum oligopeptide through a mass spectrum method, and the sequence of the 9 peptide with the highest confidence coefficient is AGDTHLGGE and also has good pancreatic lipase inhibitory activity. The production process of the plant protein is green and environment-friendly, and the product is good in safety and has good pancreatic lipase inhibition activity and cellular level lipid-lowering activity. Therefore, the golden chrysanthemum petal protein oligopeptide and 9 peptide disclosed by the invention have good effects of reducing blood fat and controlling body weight, and have application prospects in the aspects of special medical foods, health foods, functional foods, medicines and common foods.

The invention discloses a preparation method of dedifferentiated saussurea involucrata cells and anti-wrinkle tightening application of the dedifferentiated saussurea involucrata cells. The preparation method comprises the following specific steps: S1, transferring root tips of aseptic seedlings to an induction culture medium, and proliferating to form cell clusters; culturing the cell cluster in a culture dish containing a proliferation culture medium to obtain primary saussurea involucrata stem cells; s2, transferring the obtained primary saussurea involucrata stem cells into a liquid MS culture medium for suspension culture, and meanwhile, changing the illumination condition, and culturing to obtain the saussurea involucrata stem cells of which the total flavone content and the chlorogenic acid content are obviously increased. According to the method, large-scale production of saussurea involucrata stem cells is realized through a plant cell culture technology, the problem of scarcity of natural resources is solved, and rare and sustainable raw materials are realized; according to the method, unique culture conditions and inducer combination are adopted, so that the activity and the active ingredient content of the saussurea involucrata stem cells are improved, and process innovation is realized; meanwhile, the saussurea involucrata stem cells prepared by the method provide a safe raw material with anti-wrinkle and firming effects for the skin care product industry.

The application discloses a bacteriocin synthesis regulation gene plnC overexpression and a construction method and application of a recombinant plant lactis bacillus, and has the characteristics that the nucleotide sequence is shown in SEQ ID No:1 in the sequence table, and the construction method steps are as follows: 1) taking the plant lactis bacillus genomic DNA as a template, designing specific primers and performing PCR amplification; 2) purifying and double enzyme cutting the plnC gene fragment, and connecting the linear carrier pMG36e which is also double enzyme cut to obtain a pMG36e-plnC recombination cloning carrier; (3) through an electric transformation method, the pMG36e-plnC is transferred into plant lactis bacillus B1 competent cells to obtain a plnC overexpression strain B1-plnC, and the advantages are that the synthesis of the plant lactis bacillins is promoted, the plnC gene expression amount of the overexpression strain B1-plnC is increased by more than 4.5 times, and the PlnC protein content is increased by more than 1.5 times.

The present application relates to the technical field of plant breeding, and particularly relates to a molecular marker and application thereof in breeding soybean varieties with high oil content and high protein content. The molecular marker is constructed based on a SNP site; based on the genome version number Wm82.a2.v1, the SNP site is located at the 8th chromosome of soybean at the 9020004th position, and the polymorphism is T / A. The application comprises: (1) predicting or detecting the oil content or protein content of soybean; (2) identifying or breeding soybean varieties with high oil content or protein content; (3) molecular marker assisted breeding of soybean; (4) improvement of soybean varieties related to oil content or protein content; (5) improvement of soybean germplasm resources. The molecular marker provided by the present application has the characteristics of double trait synergistic correlation, strong specificity, efficient detection process and the like, and can be widely applied to the scenes of molecular marker assisted breeding of soybean, rapid evaluation of germplasm resources and the like, and provides key technical support for breeding of soybean varieties with double optimal traits.

The present invention discloses a plant lactobacillus SC256, which has been deposited in Wuhan University, China Type Culture Collection Center, with a deposit date of October 26, 2023, and a deposit number of CCTCC NO: M20232050. The strain SC256 provided by the present invention is highly resistant to artificial gastric juice and intestinal juice, and also has significant tolerance to bile salts; the strain can inhibit α-glucosidase enzyme activity, and can stimulate the secretion of insulin, and has a certain anti-diabetic effect. The strain SC256 has good hydroxyl radical scavenging ability and lipid peroxidation resistance, which can be used for the development of functional foods and living drugs, and has potential application value in diseases such as hypertension and hyperlipidemia. At the same time, the strain can also be used for solid-state fermentation of soybean meal, which can significantly increase the protein content of soybean cake, and can also be used for efficient fermentation of exopolysaccharides, and has significant application value in the biological fermentation production of exopolysaccharides.

The application discloses a kind of AbGLA1 The application discloses application of a gene in improving protein content of Agaricus bisporus, and belongs to the technical field of edible mushroom breeding. AbGLA1 The nucleotide sequence of the gene is shown as SEQ ID NO.1 or SEQ ID NO.2, and by overexpressing the gene, the protein content of fruiting bodies of Agaricus bisporus can be significantly improved. AbGLA1 The key role of the gene in protein content regulation is verified. Not only excellent germplasm resources for high-protein Agaricus bisporus breeding are provided, but also a key target for genetic improvement of protein content traits is determined.

The invention relates to the technical field of plant cultivation, in particular to a method for increasing the protein content of mulberry leaves, which comprises the following steps: after preparing a treating fluid, irrigating mulberry seeds with the treating fluid; the treating fluid is obtained by mixing 100 to 400mg of soybean lecithin and 1L of water; according to the method, the mulberry seeds are treated through the treating fluid, the mulberry seedlings with the high mulberry leaf protein content are obtained, after the obtained high-protein mulberry seedlings are transplanted for two years, the obtained mulberry leaves still maintain the high protein content, and the novel method which is easy to achieve and can effectively improve the protein content of the mulberry leaves is achieved.

The invention relates to the technical field of food processing, and particularly discloses a preparation process of high-protein spicy shredded chicken and an automatic frying pan thereof. The invention relates to a preparation process of high-protein spicy shredded chicken. The preparation process comprises the following steps: unfreezing frozen chicken, draining water, and cutting into shreds; preparing a pickling material, and putting the shredded chicken into the pickling material for pickling; frying the pickled shredded chicken in oil; frying the fried shredded chicken in an automatic frying pan in a sectional temperature control manner, wherein the sectional temperature control comprises four stages of low-temperature slow heating, medium-temperature quick frying, high-temperature stir-frying and low-temperature slow stewing; and spreading for cooling, sorting and framing, and carrying out modified atmosphere packaging. The automatic frying pan can be used for preparing high-protein spicy shredded chicken, and has the advantage of keeping the meat fresh and tender; in addition, the preparation technology has the advantages that the protein of the shredded chicken can be protected in the preparation process, nutritional ingredients such as the protein of the shredded chicken are prevented from being damaged and deteriorated, and the taste and flavor of the spicy shredded chicken are improved.

The invention discloses a high-protein pig feed capable of improving pig immunity, which is prepared from the following substances in percentage by mass: 20 to 35 percent of corn, 10 to 20 percent of bran, 2 to 6 percent of fattening fertilizer, 0.5 to 2 percent of glucose, 0.5 to 1 percent of alkali red mud, 0.1 to 0.5 percent of baking soda, 0.1 to 0.3 percent of table salt, 0.1 to 0.3 percent of mold removal agent, 0.02 to 0.1 percent of sodium selenite vitamin E, 0.02 to 0.1 percent of calcium hydrophosphate, 0.3 to 0.8 percent of ash wood and the balance of alfalfa. The invention has the following beneficial effects: 1, the protein content is high, and the cost is low; 2, the intake of trace elements is improved in daily feeding, and the immunity of the pigs is improved; and 3, safety and effectiveness.

The invention provides high-protein nut milk and a preparation method thereof, and the high-protein nut milk comprises the following raw materials in parts by weight: 130-200 parts of nut meat, 0-100 parts of white granulated sugar, 0.5-8 parts of an emulsifier, 0.1-3 parts of a stabilizer, 0.2-3 parts of sodium bicarbonate or sodium carbonate, 0.2-0.6 part of calcium salt and 0.1-2 parts of sodium citrate. The preparation method comprises the following steps: crushing and grinding the nut meat to obtain nut sauce; adding part of sodium bicarbonate or sodium carbonate and tricalcium phosphate into water to obtain a mixed solution; putting the nut sauce into the mixed solution, shearing at constant temperature, and heating to obtain nut pulp; the combination of the emulsifier, the stabilizer and the sodium citrate and part of the white granulated sugar are subjected to dry material premixing, the nut pulp is put into the mixture, the mixture is mixed with the remaining white granulated sugar, then constant-temperature shearing is conducted, pH is adjusted, the volume is constant, residues are removed, homogenizing and sterilizing are conducted, and the high-protein nut milk is obtained.

This application provides a skin-renewing composition containing an acid-enzyme peptide, comprising lactobionic acid, hexanoyl dipeptide-3-leucine, and papain; the mass ratio of lactobionic acid, hexanoyl dipeptide-3-leucine, and papain is 0.1~15:0.5~15:0.05~5. This application also provides a product comprising the above composition and its application. This application combines lactobionic acid, papain, and hexanoyl dipeptide-3-leucine for biological skin renewal, i.e., exfoliation. The three components interact synergistically, significantly increasing the content of exfoliated keratin proteins and providing excellent exfoliation effects. Furthermore, the composition provided in this application has low irritation.

The invention belongs to the technical field of biological fermentation and single-cell protein production, and particularly relates to a method for preparing a single-cell protein feed through compound strain liquid-solid combined fermentation. The method comprises the following steps: S1, liquid fermentation: respectively preparing a neurospora crassa fermentation broth, a monascus fermentation broth, a bacillus fermentation broth, a candida utilis fermentation broth and a lactic acid bacteria fermentation broth; s2, solid-state fermentation: fully mixing the prepared fermentation liquor, and then adding the mixture into a solid-state fermentation culture medium for solid-state fermentation to obtain a material; and S3, bagging the material obtained in the step S2, sealing, stacking, and carrying out anaerobic fermentation to obtain the single-cell protein feed. According to the invention, a compound strain is adopted for fermentation, full utilization of a solid-state fermentation substrate can be promoted while a large number of thalli are obtained through liquid-state fermentation, the abundance of mycoprotein can be increased through coordinated fermentation, the content of restrictive amino acids in livestock and poultry can be increased, and the nutritional value of the mycoprotein can be further increased.

This invention discloses a method for biotechnology-based conversion of livestock and poultry manure into animal feed, belonging to the field of agricultural waste resource utilization. The method includes: solid-liquid separation and crushing of livestock and poultry manure, adjusting the moisture content to 65%~75%; applying a pulsed electric field to treat the manure, resulting in a microbial cell breakage rate ≥70% and a cellulose crystallinity reduction ≥15%; adding a complex enzyme preparation composed of laccase, manganese peroxidase, xylanase, and lysozyme for enzymatic hydrolysis; after cooling, inoculating a mixed flora of thermophilic Bacillus stearothermophilus and Kluyveromyces martensii acclimated with sodium acetate and phenol; conducting a two-stage fermentation, with an aerobic stage followed by closed, static fermentation; and finally, low-temperature vacuum drying and pulverization to obtain feed protein powder. This invention, through the synergistic effect of pulsed electric field, complex enzyme, and acclimated microbial flora, achieves efficient, safe, and rapid feed utilization of livestock and poultry manure, with a total processing time ≤72 hours, a crude protein content of 28%~35%, and antibiotic residue ≤0.2 mg / kg.

The invention discloses a method for modifying cellulosic fibers by using a quinoa protein finishing agent, and relates to the technical field of preparation of quinoa protein finishing agents, the preparation method comprises the following steps: extraction of quinoa protein liquid: extracting protein from quinoa by physical and chemical methods to obtain the quinoa protein liquid; performing modification treatment on the quinoa protein liquid: performing cross-linking modification on the quinoa protein liquid by using polyvinyl alcohol, wherein the mass ratio of the polyvinyl alcohol to the quinoa protein liquid is 0.02: 1 to 0.12: 1; spinning by a wet spinning-stretching method: mixing the modified quinoa protein solution with a cellulose fiber solution, and spinning by adopting the wet spinning-stretching method to form nascent filaments; post-treatment: performing acid pickling, desulfurization, water washing, oiling, dehydration and drying on the nascent filaments to obtain the quinoa protein cellulose fibers. According to the method for modifying the cellulosic fibers by utilizing the quinoa protein finishing agent, the problem that quinoa protein is difficult to combine with the cellulosic fibers in the prior art is solved, and an environment-friendly and efficient fiber modification scheme is provided.

The invention discloses an environment-friendly hermetia illucens breeding feed and a preparation method thereof, and mainly relates to the technical field of invertebrate breeding. Compared with the prior art, the environment-friendly hermetia illucens breeding feed can improve the high protein content of hermetia illucens bodies and reduce breeding stink at the same time, conversion and absorption of nutrient substances in the feed are jointly promoted by using rotten vegetable and fruit raw materials, saccharomyces cerevisiae, corn flour, wheat bran, peanut bran and composite strains, generation of beneficial metabolites is increased, and the breeding efficiency of hermetia illucens is improved. The deodorant composed of citrus flavone, moringa oleifera leaf protein and sodium alginate can effectively adsorb and inhibit the emission of malodorous gases such as ammonia gas, and improve the breeding environment.

This invention belongs to the field of bio-fermentation feed technology, specifically involving a two-step fermentation method for producing microbial protein bio-feed from corn husks. The invention provides a method for preparing microbial protein bio-feed, specifically: inoculating a first fermentation substrate with Aspergillus niger inoculum for a first fermentation to obtain a starter culture; and inoculating a second fermentation substrate with a compound microbial agent for a second fermentation to obtain the microbial protein bio-feed. The preparation method described in this invention can maximize the degradation of cellulose and hemicellulose in corn husk raw materials while considering cost, and increase its protein content through secondary fermentation, providing the feed industry with a method for preparing high-quality protein feed.

The invention relates to the technical field of food processing, and in particular to a method for preparing a microalgae plant milk beverage, comprising: S1, selecting fresh microalgae in which protein accounts for ≥40% of cell dry weight, oil accounts for ≤15% of cell dry weight, and starch accounts for ≤30% of cell dry weight; S2, preparing algae pulp with drinking water, breaking algae cells with a high-pressure homogenizer to obtain broken algae pulp; S3, adding water to dilute the broken algae pulp, adding 350-5000 units / kg of cellulase, 500-50000 units / kg of protease, 500-3000 units / kg of amylase and 1000-3000 units / kg of saccharifying enzyme to carry out enzymolysis based on the weight of the diluted algae pulp, and after the reaction is completed, adding an additive of less than 5g / 100mL, stirring and mixing evenly; S4, heating to 50-80°C, and homogenizing again; S5, sterilizing and filling to obtain a finished product. The present invention adopts microalgae with high protein content to prepare a plant milk beverage, and the protein / oil or protein / starch ratio of the plant milk beverage is reasonable, thereby making up for the problem of low protein / calorie ratio of the plant milk beverage.

The invention provides a method for extracting fresh moringa oleifera leaf protein by adding lactobacillus plantarum for fermentation, which comprises the following steps: cutting fresh moringa oleifera leaves into sections as raw materials to extract and process leaf protein, and sequentially carrying out the procedures of pretreatment, pulping, leaf pulp separation, fermentation, centrifugal separation, freeze drying and the like to realize complete extraction of the moringa oleifera leaf protein. The processing process is safe, simple and convenient, byproducts can be recycled for the second time, no waste is generated, and the method is environment-friendly and pollution-free; the leaf protein concentrate extraction rate can reach 59.25%, the protein content can reach 54.42%, and the leaf protein feed is a high-quality protein feed.

The invention discloses thick-wall stuffed bean curd with high shape retention, high moisture and high nutrition and a preparation method of the thick-wall stuffed bean curd, and belongs to the technical field of food processing. The nut protein is added into the bean curd skin, and meanwhile, the preparation process is controlled to realize the effects of high moisture content, high nutritional value, high shape retention and multiple stuffing choices of the stuffed bean curd. Specifically, the stuffed bean curd prepared by the preparation method does not need to be shaped in a deep-frying or water boiling manner, and can be shaped only by a pressing process; various types of fillings (meat products, aquatic products, vegetable fillings and the like) can be added, and a shaping agent does not need to be added into the fillings; and meanwhile, the pot can be suitable for various cooking modes such as frying, frying and boiling. In addition, the stuffed bean curd is low in fat, high in protein, high in elasticity and diversified in flavor.

The invention discloses a high-protein alkalized corn feed preparation process, which comprises: S1, lime mixed liquid preparation: placing 3-4 parts of quicklime in a filter cartridge, then descending into a mixing box, then injecting 200-300 parts of urea mixed liquid, the urea mixed liquid being 30% of urea and 70% of water, and stirring uniformly; the quick lime is stirred and crushed by the stirring blades, so that the quick lime is fully mixed with the urea mixed solution, and the lime and urea mixed solution is prepared. According to the preparation process of the high-protein alkalized corn feed, straw can be crushed and stored in advance through the arrangement of the two mechanisms, a lime water mixed solution is prepared, and the corn straw cut into small sections and the lime mixed solution are soaked and alkalized, so that the protein content of the alkalized feed is effectively improved by utilizing urea in the lime mixed solution; therefore, the feed has the characteristic of high protein, the digestion of livestock is improved, and the protein content required by the livestock is met.

The invention discloses a method for treating waste bacterial liquid in the process of preparing glucurolactone through a fermentation method, and relates to the technical field of glucurolactone production, the waste bacterial liquid concentrated through a ceramic membrane is taken, the waste bacterial liquid is collected, the volume concentration of escherichia coli in the waste bacterial liquid is 20-50%, and the total phosphorus content is 1500-2000 ppm; adding a flocculating agent into the filtrate, stirring, standing, then adding diatomite, and uniformly stirring to obtain a mixture, the flocculating agent comprising citric acid and chitosan; the mixture enters a diaphragm filter press, and a solid filter cake is obtained after filter pressing is finished. The problem of post-treatment of the waste bacterial liquid is solved, the bacterial liquid is high in protein content, easy to decay and stink, incapable of being placed for a long time, not easy to transport and incapable of being subjected to pollution discharge treatment, the bacterial liquid is prepared into a solid state through an effective flocculation method, the environment can be improved, filter cakes can be used for preparing organic fertilizer, and enterprise benefits are increased.

The application belongs to the technical field of genetic engineering, and discloses a method for improving biomass and cell protein content of Bacillus amyloliquefaciens by knocking out global transcription factor codY. The engineering strain is obtained by taking Bacillus amyloliquefaciens HZ-12 as a starting bacterium and knocking out the global transcription factor coding gene codY, and the biomass and cell protein content of the Bacillus amyloliquefaciens engineering bacterium HZ-12ΔcodY are increased by 37.44% and 6.88% respectively relative to those of the control strain HZ-12.

The invention discloses a bacterium-enzyme synergistic debitterizing pea protein beverage and a preparation method thereof, and belongs to the technical field of food processing. According to the method, pea protein is adopted as a raw material, enzymolysis of alkaline protease and flavourzyme and fermentation of composite strains of lactobacillus plantarum, streptococcus thermophilus and lactobacillus helveticus are utilized to achieve a synergistic effect, and compared with fermentation by independently using lactic acid bacteria, debitterizing time is shorter, overall debitterizing efficiency is higher, sensory evaluation is better, and overall acceptability is high; pea-related peculiar smell-related volatile compounds, including hexanal, 2-nonanone, (E, E)-2, 4-nonadienal, 2-amyl furan, (E, E)-2, 4-decadienal and the like, of the prepared product are reduced or completely eliminated. The debittered pea protein beverage and the preparation method thereof provided by the invention are beneficial to widening the utilization way of pea protein and promoting the development of the pea protein beverage industry in China.

The application relates to the technical field of skin bacteriostatic liquid, and discloses a skin bacteriostatic liquid containing plant extracts and a preparation method thereof, which comprises the following raw materials in parts by mass: composite plant extracts containing nanocrystalline particles 15-20 parts, glycerol 3-4 parts, sodium hyaluronate 0.1-0.2 parts and purified water 75-85 parts. The composite plant extracts containing nanocrystalline particles, glycerol, sodium hyaluronate and purified water are mixed to obtain the skin bacteriostatic liquid, which has the advantages of strong bacteriostasis, long-acting bacteriostasis, mildness and no irritation, and can repair the skin barrier, realize long-acting skin bacteriostasis, reduce the repeated occurrence of skin inflammation, and relieve inflammatory skin aging.