10results about How to "High protein content" patented technology

The application discloses a bacteriocin synthesis regulation gene plnC overexpression and a construction method and application of a recombinant plant lactis bacillus, and has the characteristics that the nucleotide sequence is shown in SEQ ID No:1 in the sequence table, and the construction method steps are as follows: 1) taking the plant lactis bacillus genomic DNA as a template, designing specific primers and performing PCR amplification; 2) purifying and double enzyme cutting the plnC gene fragment, and connecting the linear carrier pMG36e which is also double enzyme cut to obtain a pMG36e-plnC recombination cloning carrier; (3) through an electric transformation method, the pMG36e-plnC is transferred into plant lactis bacillus B1 competent cells to obtain a plnC overexpression strain B1-plnC, and the advantages are that the synthesis of the plant lactis bacillins is promoted, the plnC gene expression amount of the overexpression strain B1-plnC is increased by more than 4.5 times, and the PlnC protein content is increased by more than 1.5 times.

The present application relates to the technical field of plant breeding, and particularly relates to a molecular marker and application thereof in breeding soybean varieties with high oil content and high protein content. The molecular marker is constructed based on a SNP site; based on the genome version number Wm82.a2.v1, the SNP site is located at the 8th chromosome of soybean at the 9020004th position, and the polymorphism is T / A. The application comprises: (1) predicting or detecting the oil content or protein content of soybean; (2) identifying or breeding soybean varieties with high oil content or protein content; (3) molecular marker assisted breeding of soybean; (4) improvement of soybean varieties related to oil content or protein content; (5) improvement of soybean germplasm resources. The molecular marker provided by the present application has the characteristics of double trait synergistic correlation, strong specificity, efficient detection process and the like, and can be widely applied to the scenes of molecular marker assisted breeding of soybean, rapid evaluation of germplasm resources and the like, and provides key technical support for breeding of soybean varieties with double optimal traits.

The application discloses a kind of AbGLA1 The application discloses application of a gene in improving protein content of Agaricus bisporus, and belongs to the technical field of edible mushroom breeding. AbGLA1 The nucleotide sequence of the gene is shown as SEQ ID NO.1 or SEQ ID NO.2, and by overexpressing the gene, the protein content of fruiting bodies of Agaricus bisporus can be significantly improved. AbGLA1 The key role of the gene in protein content regulation is verified. Not only excellent germplasm resources for high-protein Agaricus bisporus breeding are provided, but also a key target for genetic improvement of protein content traits is determined.

This application provides a skin-renewing composition containing an acid-enzyme peptide, comprising lactobionic acid, hexanoyl dipeptide-3-leucine, and papain; the mass ratio of lactobionic acid, hexanoyl dipeptide-3-leucine, and papain is 0.1~15:0.5~15:0.05~5. This application also provides a product comprising the above composition and its application. This application combines lactobionic acid, papain, and hexanoyl dipeptide-3-leucine for biological skin renewal, i.e., exfoliation. The three components interact synergistically, significantly increasing the content of exfoliated keratin proteins and providing excellent exfoliation effects. Furthermore, the composition provided in this application has low irritation.

This invention discloses a method for biotechnology-based conversion of livestock and poultry manure into animal feed, belonging to the field of agricultural waste resource utilization. The method includes: solid-liquid separation and crushing of livestock and poultry manure, adjusting the moisture content to 65%~75%; applying a pulsed electric field to treat the manure, resulting in a microbial cell breakage rate ≥70% and a cellulose crystallinity reduction ≥15%; adding a complex enzyme preparation composed of laccase, manganese peroxidase, xylanase, and lysozyme for enzymatic hydrolysis; after cooling, inoculating a mixed flora of thermophilic Bacillus stearothermophilus and Kluyveromyces martensii acclimated with sodium acetate and phenol; conducting a two-stage fermentation, with an aerobic stage followed by closed, static fermentation; and finally, low-temperature vacuum drying and pulverization to obtain feed protein powder. This invention, through the synergistic effect of pulsed electric field, complex enzyme, and acclimated microbial flora, achieves efficient, safe, and rapid feed utilization of livestock and poultry manure, with a total processing time ≤72 hours, a crude protein content of 28%~35%, and antibiotic residue ≤0.2 mg / kg.

The application belongs to the technical field of genetic engineering, and discloses a method for improving biomass and cell protein content of Bacillus amyloliquefaciens by knocking out global transcription factor codY. The engineering strain is obtained by taking Bacillus amyloliquefaciens HZ-12 as a starting bacterium and knocking out the global transcription factor coding gene codY, and the biomass and cell protein content of the Bacillus amyloliquefaciens engineering bacterium HZ-12ΔcodY are increased by 37.44% and 6.88% respectively relative to those of the control strain HZ-12.

This invention discloses a ZmAAP14 protein and gene for increasing the protein content of corn kernels. The gene sequence is shown in SEQ ID NO.1, and the amino acid sequence is shown in SEQ ID NO.2. This invention constructs... ZmAAP14 Gene overexpression vectors, overexpression in maize ZmAAP14 Genes, determined ZmAAP14 Genes can increase the protein content of corn kernels and can be used to breed new high-protein corn varieties.

The invention provides a yeast hydrolysate as well as a preparation method and application thereof, the yeast hydrolysate comprises the following components in percentage by mass: 35-65% of true protein, aromatic amino acid and sulfur-containing amino acid, and the content ratio of the aromatic amino acid to the sulfur-containing amino acid is 2-5; the ratio of the content of the components with the weight-average molecular weight of 200-2000Da to the content of the components with the weight-average molecular weight of more than 2000Da is 4-10, and the ratio of the content of the components with the weight-average molecular weight of 200-2000Da to the content of the components with the weight-average molecular weight of less than 200Da is 4-9; the yeast hydrolysate further comprises disodium nucleotide, the disodium nucleotide comprises disodium inosinate, disodium guanylate, disodium cytidine monophosphate and disodium uridinemonophosphate, and the ratio of the mass sum of the disodium inosinate and the disodium guanylate to the mass sum of the disodium cytidine monophosphate and the disodium uridinemonophosphate to the disodium nucleotide is 1: (0.6-1.2): (1.6-2.2). The feed additive is beneficial for further improving the feed intake of the piglets and reducing the disease incidence of the piglets.

The invention discloses a device facilitating dough lamination, a meal package and a preparation method of the meal package, and belongs to the technical field of dough lamination equipment, the device comprises a conveying device, a lamination mechanism and a cutting mechanism, the lamination mechanism comprises a fifth motor, two conveying belts, a lifting assembly and a pressing plate, the conveying belts can stack dough at the center of the dough, and the pressing plate is arranged on the conveying device; the lifting assembly can drive the pressing plate to press the stacked dough; dough is conveyed to the conveying belt through the conveying device, the edge parts of the dough are stacked through the conveying belt, the lifting assembly drives the pressing plate to move up and down, the dough is pressed through the pressing plate, manual stacking of the dough is not needed, and safety is improved; the dough at the edge part is separated by the separating assembly and falls into the collecting box, so that reutilization is facilitated, and waste of the dough is avoided. Meanwhile, the meal package and the preparation method thereof solve the problem of keeping the red beans beautiful, and are healthy and sugar-controlled.

This invention discloses a CoQ10 nanoemulsion yogurt and its preparation method, belonging to the field of food processing technology. The preparation method of the CoQ10 nanoemulsion yogurt of this invention includes the following steps: mixing whey protein with water, hydrating, and thermally polymerizing to obtain a polymerized whey protein solution; mixing the polymerized whey protein solution with a CoQ10 solution, emulsifying, and evaporating to remove the solvent from the CoQ10 solution to obtain a PWP-CoQ10 nanoemulsion; mixing the PWP-CoQ10 nanoemulsion with milk to obtain a precursor liquid, emulsifying, heating, and then adding a starter culture for fermentation to obtain the CoQ10 nanoemulsion yogurt. The CoQ10 nanoemulsion yogurt prepared by this invention possesses both the antioxidant properties of CoQ10 and the thickener function of whey protein; compared with ordinary yogurt, it has the characteristics of high protein content, good antioxidant effect, and no additional thickener added, expanding the application of CoQ10 in the food field and showing good prospects for promotion and application in the field of functional yogurt.