65 results about "Lactococcus lactis" patented technology

The invention relates to the technical field of microorganisms, and particularly discloses a nitrogen-fixing and cadmium-reducing microbial agent as well as a preparation method and application thereof. The microbial agent consists of bacillus pseudomycoides, lactococcus lactis, azotobacter chroococcum and biochar, wherein the biochar is obtained by pyrolyzing and carbonizing rice straws. The bacillus pseudomycoides can reduce the bio-availability of soil cadmium; lactococcus lactis can secrete a large number of biological active substances such as exopolysaccharides and enzymes, and the activity of other microorganisms is stimulated and improved; azotobacter chroococcum converts nitrogen in air into ammonium, amino acid and peptide which can be utilized by plants and promotes plant growth, and released metallothionein contains free sulfydryl which is combined with Cd ions, so that a stable sulfide compound is formed. The biological carbon adsorbs and fixes the three bacteria, so that the microorganisms can continuously and effectively play a role and have a mutual synergistic effect, the fixation of Cd in the soil is further enhanced, the availability of nitrogen is improved, and the plant growth is effectively promoted.

The invention relates to a polygonatum sibiricum probiotic compound preparation with an aging delaying effect and application thereof. The compound preparation comprises a polygonatum sibiricum extract and a probiotic composition, the bacterial strains in the probiotic composition comprise a combination of a bifidobacterium animalis subsp. Lactis BLa36 bacterial strain, a bifidobacterium longum BL21 bacterial strain, a bifidobacterium adolescentis BAC30 bacterial strain, a lactobacillus plantarum Lp90 bacterial strain, a lactobacillus casei LC89 bacterial strain, a bifidobacterium breve BBr60 bacterial strain and a pediococcus acidilactici CCFM7902 bacterial strain. After the rhizoma polygonati component and the probiotic component are combined for use, the rhizoma polygonati component and the probiotic component have potential synergistic effects in the aspects of improving the intestinal flora structure, regulating the intestinal environment, delaying senescence, reducing inflammation-related symptoms and the like. Moreover, further, various probiotic strains are compounded and combined for use, so that the capability of a related compound preparation in the aspect of delaying various symptoms of senescence can also be improved in a combined manner.

The invention relates to selenium-enriched pediococcus acidilactici Se34 with anti-oxidation, cholesterol-lowering and anti-tumor effects and application of the selenium-enriched pediococcus acidilactici Se34. The pediococcus acidilactici Se34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number of the pediococcus acidilactici Se34 is CGMCC No.33933, and the preservation date of the pediococcus acidilactici Se34 is March 24, 2025. The pediococcus acidilactici Se34 provided by the invention integrates excellent probiotic characteristics (acid-resistant bile salt, safe and colonizable), efficient selenium enrichment capacity and remarkably enhanced activity of resisting oxidation, reducing cholesterol and inhibiting specific cancer cell proliferation and metastasis after selenium enrichment. The strain and the selenium-rich product thereof have important application value in the fields of developing functional foods (selenium-rich probiotic products), antioxidant / cholesterol-reducing health-care foods, potential auxiliary anticancer biological preparations or prodrugs and the like.

The invention discloses a streptomyces Targetron gene targeting vector as well as a construction method and application of the streptomyces Targetron gene targeting vector. According to the invention, an II-type intron Ll.LtrB from lactococcus lactis is cloned to an escherichia coli-streptomyces shuttle plasmid to construct a streptomyces genetic manipulation tool-vector pSC30 based on targeting of the II-type intron, and gene targeting in streptomyces coelicolor is successfully realized by using the tool. Screening a biosynthetic gene cluster of red pigment in streptomyces roseosporus; and activating a biosynthetic gene cluster of janus doxorubicin in streptomyces spinosus. According to the invention, the conversion rate and the gene targeting efficiency of the Ll.LtrB intron in streptomyces with high GC content are effectively improved, and a new tool is provided for construction of a gene mutation library.

This invention aims to address the technical bottlenecks of existing milk curd products, such as weak functional activity, unclear process parameters, and unstable flavor and quality. It utilizes *Pediococcus pentosaceus* HM4, *Streptococcus thermophilus* HM6, *Lactococcus lactis* JY4, and *Lactobacillus plantarum* NC1, employing these four strains in an optimal ratio as a compound fermentation agent to achieve synergistic effects. Using milk and goji berries as raw materials, the fermentation process of milk curds and goji berry milk curds was systematically optimized through single-factor experiments, Plackett-Burman experiments, and response surface methodology. The physicochemical indicators and flavor characteristics of the products under different process conditions were analyzed. The obtained milk curds achieved a DPPH free radical scavenging rate of 91.38%–94.15%, providing technical support for the high-value utilization of milk curds and enriching the variety of functional dairy products.

A cultivation medium for Auricularia auricula that can increase riboflavin content, belonging to the technical field of breeding and cultivation of high-riboflavin Auricularia auricula strains, is composed of basswood sawdust, quicklime, gypsum powder, purified water and a fermented product. The fermented product, calculated by weight, is prepared by mixing 10 - 12 parts of Chrysanthemum morifolium Ramat., 5 - 8 parts of Ginkgo biloba leaves, 3 - 6 parts of Glycyrrhiza uralensis Fisch., 20 - 30 parts of pine cones and 15 - 18 parts of walnut shells into a mixed powder 1, adding chaff, corn flour and sucrose powder to form a mixed powder 2, and then fermenting with Hansenula anomala, Lactococcus lactis and Bacillus licheniformis after sterilization. The cultivation medium prepared by the present invention effectively increases the riboflavin content in Auricularia auricula, reaching more than 2 mg / 100 g of dry Auricularia auricula, shortens the cultivation cycle of Auricularia auricula, and at the same time, the dry Auricularia auricula yield reaches more than 120 g / bag. The above cultivation method can be used for the seedling cultivation of high-riboflavin Auricularia auricula strains, and high-quality strains of high-riboflavin Auricularia auricula can be obtained through the cultivation with this cultivation medium.

The invention provides a strain capable of resisting radiation damage as well as a product and application thereof, and relates to the technical field of probiotics capable of resisting radiation damage. The probiotic strains are separated from healthy breast milk and comprise lactobacillus gasseri LG02, lactococcus lactis LL6 and bifidobacterium adolescentis BA18, and the three probiotic strains can improve the survival rate after ionizing radiation; the LG02 or LL6 strain relieves damage of ionizing radiation to tissue structures of the heart, the liver, the spleen, the lung, the kidney, the stomach, the small intestine, the colon and the femur, the physiological function of an organism is maintained, and recovery of the tissue structures is accelerated; the LG02 or LL6 strain accelerates the body repair after radiotherapy, reduces the side reactions of diarrhea, stomachache, constipation and cough, and reduces the incidence rate of hematochezia and radiation pneumonitis; the LG02 or LL6 strain increases or recovers the number of leukocytes, and improves the hematopoietic ability and immunity of the organism; the LG02 or LL6 strain has the effects of inhibiting harmful bacteria, improving the anti-infection and anti-inflammation capability of the body and improving the resistance; the LG02 or LL6 strain can improve the oxidation resistance of the body and enhance the aging resistance of the body.

The invention belongs to the technical field of biology, and particularly relates to a recombinant strain of high-yield riemerella anatipestifer bacteriophage lyase, a mutant strain, a construction method and application. The invention provides a coding gene of riemerella anatipestifer bacteriophage lyase, and further constructs recombinant lactococcus lactis of high-yield riemerella anatipestifer bacteriophage lyase. The mutant strain provided by the invention is obtained by taking the recombinant lactococcus lactis as a starting strain through a mutation breeding technology and a specific high-throughput screening strategy, has higher secretory expression quantity of riemerella anatipestifer bacteriophage lyase, is good in hereditary stability in an antibiotic-free culture medium, and has a good application prospect. The method is suitable for industrial efficient production of active lyase. The riemerella anatipestifer bacteriophage lyase expressed by the mutant strain has an obvious splitting effect on riemerella anatipestifer in vitro, has obvious prevention and treatment effects on riemerella anatipestifer infection in vivo, and can effectively improve the survival rate of infected chicks and remove pathogenic bacteria in vivo.

The application discloses a kind of complex fermentation inoculant for improving equol production, fermentation liquor and application thereof, belong to microbial fermentation technical field.The complex fermentation inoculant of the application, streptococcus thermophilus BLCC2-0025 and lactococcus lactis MP1001 have high β-glucosidase activity, and low activity glycoside form is converted into high activity daidzein;Salivary lactobacillus LS1101 and lactobacillus gregius 0010 have the activity of further converting daidzein into equol;Four strains of bacteria are used in combination, which can synergistically improve the content of equol.The fermentation liquor prepared by fermenting pueraria extract with the complex fermentation inoculant of the application can target regulate intestine-ovary axis, significantly improve the reproductive function of POF mice, relieve ovarian oxidative stress and accurately regulate intestinal flora balance, providing a safe and effective preparation and application scheme for non-hormone intervention of premature ovarian failure.

The invention provides a three-dimensional microbial flora biological hybrid system capable of efficiently generating electricity by utilizing lignocellulose hydrolysate, and belongs to the technical field of biological batteries. According to the invention, an artificial microbial flora SLB composed of S.oneidensis, Lactococcus lactis and Bacillus subtilis is preliminarily constructed, the bacteria in the artificial microbial flora SLB are respectively subjected to genetic engineering modification, then a carbon cloth anode is replaced by a carbon felt anode, a dispersion liquid GO is added, the electricity generation and conductivity of an anode electroactive biological membrane are further optimized, and the electrochemical performance of the anode electroactive biological membrane is further improved. And finally, a three-dimensional microbial flora biological hybrid system S7L1B1 (at) CFamp capable of directly utilizing the lignocellulose hydrolysate to generate power is constructed. And GO. According to the present invention, the MFC technology is introduced, such that the MFC technology has good lignocellulose hydrolysate degradation and biological power generation capacity, such that the new approach is developed for the high-value utilization of the lignocellulose resource, the solid theoretical basis and the practical guidance are provided, and the application development of the MFC technology in the lignocellulose resource conversion field is promoted.

The application discloses a pure oat-based fermented beverage and a preparation method thereof, and belongs to the technical fields of microorganisms and fermentation. The pure oat beverage is used as a fermentation raw material, and lactococcus lactis is added to perform fermentation, so that the content of beta-glucan in the oat beverage is increased by 40% to 70%, and the taste of the oat beverage is improved. The ratio of sweet amino acids to bitter amino acids is (1-1.2):1. Compared with other similar products obtained by fermentation of pure plant bases, the product is natural and delicious, has a long survival time in storage, does not need complex processes and expensive equipment, has low product manufacturing cost, and has good practical value and application prospect.

The application relates to the field of food biotechnology, and discloses a low-salt bean paste production process based on segmented temperature control fermentation, which is made of fava bean koji, water, edible salt, sodium hexametaphosphate, lactococcus lactis nisin, edible alcohol, granulated coarse granular calcium carbonate and glucono-delta-lactone. The production process comprises the following steps: constructing a composite matrix, and first performing buffer-type medium-temperature enzymolysis; then adding a glucono-delta-lactone aqueous solution in a flow manner, utilizing the stoichiometric coupling reaction of the glucono-delta-lactone aqueous solution and the granulated coarse granular calcium carbonate to generate carbon dioxide in situ to construct a micropore structure and regulate the pH value of the system; and finally, connecting a Zygosaccharomyces rouxii to perform low-temperature aroma-producing after-ripening. Through the synergistic effect of the components, the application effectively solves technical problems in the low-salt fermentation process, such as low protein solubility, easy compaction of the matrix and breeding of miscellaneous bacteria, and improves the amino acid nitrogen content and protein hydrolysis rate of the finished product.

The invention relates to the technical field of food processing, in particular to a processing method for cooked mutton, which comprises the following steps: obtaining a fresh mutton raw material; performing pretreatment before fermentation on the fresh mutton raw material; adding a compound bacteria solution into the pretreated fresh mutton raw material, and fermenting to hydrolyze goaty-flavor substances in the mutton to obtain fermented mutton; immersing the fermented mutton into a composite gel solution and carrying out vacuum impregnation so as to selectively remove fermented free mutton smell substances, so as to obtain mutton smell removed mutton; and pickling the mutton with the removed mutton smell with ingredients, and cooking the mutton at 80-85 DEG C for 10-15 minutes to obtain a cooked mutton finished product, wherein the compound bacteria liquid consists of staphylococcus carnosus, pediococcus pentosaceus, lactococcus lactis and a calcium chloride solution. According to the invention, mutton-smell-causing short-chain branched fatty acid in mutton is degraded by a microbial mutton-smell-reducing method, and long-chain branched fatty acid with physiological activity is retained at the same time.

This invention provides a set-type yogurt with a milk skin layer and its production method, relating to the field of dairy product technology. The invention involves mixing raw milk with white sugar and a stabilizer, cooling it, and then adding functional excipients to obtain base material 1. The raw milk is then centrifuged and defatted to prepare light cream, yielding base material 2. Base material 1 and base material 2 are mixed, inoculated with a complex microorganism, and subjected to static fermentation followed by low-temperature ripening. The stabilizer consists of acetylated distarch adipate, κ-carrageenan, and diacetyl tartaric acid mono- and diglycerides. The complex microorganisms consist of *Streptococcus thermophilus* subsp. *salicylate*, *Lactococcus lactis* subsp. *lactophores*, and *Bifidobacterium animalis* subsp. *lactophores*. The stabilizer enhances gel water retention and reduces whey separation, allowing the light cream to float and form a uniform and dense milk skin layer. The static fermentation and low-temperature ripening of the complex microorganisms optimize texture and flavor. The milk skin layer of this invention's set-type yogurt is intact and uniform in thickness, the yogurt curd is delicate, whey separation is minimal, and the taste is rich and mellow.

The invention relates to the technical field of fermented dairy products, and discloses a fermented animal and plant double-protein aerated mousse and a preparation method thereof, and the aerated mousse is prepared from the following raw materials by weight: 300-700 parts of animal milk; 100 to 500 parts of plant milk; 40 to 100 parts of a sweetening agent; 100 to 200 parts of single cream; the compound thickening emulsifier is prepared from gelatin, agar, locust bean gum and lactic acid glycerol fatty acid ester according to the weight ratio of (10 to 25): (1 to 5): (2 to 8): (2 to 10); the fermentation agent is 150 to 250 U / T, and the fermentation agent comprises streptococcus salivarius subsp. Thermophilus, lactobacillus delbrueckii subsp. Bulgaricus, lactobacillus plantarum and lactococcus lactis subsp. Cremoris; the compound biological enzyme comprises lipase, glutamine transaminase and lactase. The aerated fermented milk which is fluffy, smooth and soft in texture is obtained, the obtained aerated fermented milk contains high viable count, the tissue state is stable within the shelf life, no gas overflows, and the aerated fermented milk has the sour and sweet taste of the fermented milk and the soft mousse cake taste.

The invention relates to the technical field of minced fillet deodorization, and discloses a minced fillet deodorization process for fish ball production, which comprises the following steps: (1) cleaning and dehydrating a minced fillet raw material, and controlling the water content at 80-95% after the raw material is dehydrated to obtain a primary minced fillet raw material; (2) activating a composite fungus material, adding the activated composite fungus material into the primary minced fillet raw material, uniformly stirring, fermenting at 28 DEG C for 50-60 minutes, heating to 30 DEG C, and fermenting for 50-60 minutes; wherein the composite fungus material comprises rhizomucor miehei, kluyveromyces marxianus, lactococcus lactis and saccharomyces cerevisiae; and (4) heating the minced fillet obtained in the step (3) to 65-68 DEG C, keeping the temperature for 2-3 minutes, rinsing with ice water, and dehydrating to obtain the fishy smell removed minced fillet. According to the method, the effect of removing the fishy smell of the minced fillet can be improved.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a compound microbial agent for fermenting sugarcane yellow leaves. The compound microbial agent is prepared from lactobacillus plantarum R4-30, lactobacillus acidophilus G32, lactobacillus fermentum R6-11, lactococcus lactis G3-1, leuconostoc citreum G7-13, candida boidinii G90-9 and pichia kudriavzevii G90-14, and the compound microbial agent is prepared from lactobacillus plantarum R4-30, lactobacillus acidophilus G32, lactobacillus fermentum R6-11, lactococcus lactis G3-1, leuconostoc citreum G7-13 The preparation method of the composite microbial agent comprises the following steps: (1) activation and rejuvenation of strains; and (2) preparation of the composite microbial agent: uniformly mixing single strain culture solutions according to a mass ratio to obtain the composite microbial agent. After the compound microbial agent provided by the invention is used for fermenting the sugarcane yellow leaves for 8 days, the fermented feed is rich in nutrition and fragrant in smell, the content of crude protein in the fermented feed is increased, the number of moulds is reduced, and the content of butyric acid, neutral detergent fibers and hemicellulose is reduced.

The invention discloses pediococcus acidilactici capable of producing cellulase and application of the pediococcus acidilactici, the preservation number of a pediococcus acidilactici strain Y2 is CCTCC M 20251203, and the 16S rDNA sequence of the pediococcus acidilactici strain Y2 is as shown in SEQ ID NO: 1. Experiments show that the Y2 bacterium and cane sugar composite addition group synchronously optimizes the two processes of fermentation and degradation through ingenious cooperation of microorganisms and substrates. A stable acid environment rich in lactic acid is created, decay is inhibited, meanwhile, a fiber barrier is remarkably broken, and the nutritional value is improved. Through the synergistic effect of the Y2 bacteria and the cane sugar, obvious advantages are shown in the aspects of fermentation efficiency, fermentation environment optimization and nutritional ingredient improvement, and good technical application prospects are achieved.

The invention relates to the technical field of flavor agents, in particular to a cream type bacterium-enzyme co-promoted cheese flavor agent with good sensory characteristics and a preparation method of the cream type bacterium-enzyme co-promoted cheese flavor agent. The flavor agent provided by the invention is composed of hydrolase and active lactic acid bacteria, the hydrolase is prepared from Protease AY 'Amano' 400SD protease, the active lactic acid bacteria are prepared from lactococcus lactis BL-19 (preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC 16242), and the fermentation substrate is cream.

The present invention provides a Salmonella - specific antigen fusion protein and its recombinant Lactococcus lactis. The antigen fusion protein is a Salmonella OmpC and Flic antigen fusion protein, and its amino acid sequence is SEQ ID NO: 1. The fusion polypeptide provided by the present invention can be used as an antigen to prepare a vaccine, and the vaccine prepared can provide highly efficient immune protection after immunizing animals. Moreover, the provided fusion polypeptide has no allergenicity and will not cause allergy in immunized animals, ensuring its safety as a vaccine.

The application relates to a production method and application of combined fermentation of bupleurum and lonicera japonica residues by bacillus velezensis and pediococcus acidilactici, and belongs to the technical field of biotechnology. The bupleurum and lonicera japonica residues are treated by adopting a process of enzymolysis first and then fermentation, wherein the composite enzyme comprises cellulase, beta-glucanase, xylanase, beta-mannanase and phytase; and the fermentation bacteria comprise bacillus velezensis and pediococcus acidilactici. The fermented bupleurum and lonicera japonica residues provided by the application have a high release amount of active ingredients (chlorogenic acid, forsythoside and baicalin), and the addition ratio in broiler chickens is 2.0%-4.0%. The application effect includes improving growth performance, improving the levels of anti-inflammatory factor IL-10 and serum immunoglobulin IgM of the body, improving the muscle quality and flavor characteristics of the breast muscle, and improving the contents of inosinic acid, intramuscular protein, flavor amino acid and total amino acid. The product can not only improve the yield and quality of livestock products, but also relieve the pressure on the environment caused by solid waste residues, and has a good market prospect.

The invention discloses application of pediococcus acidilactici NR combined with a compound enzyme preparation in preparation of a wheat bran fermentation agent and a fermentation method of the pediococcus acidilactici NR combined with the compound enzyme preparation, and belongs to the fields of fermentation engineering and feed processing. The invention provides a method for improving the protein content and the protein digestibility of fermented wheat bran through synergistic fermentation of pediococcus acidilactici and a compound enzyme preparation. The acid soluble protein content and the in-vitro protein digestibility of the wheat bran are remarkably improved through a fermentation technology. In an actual animal feeding test, the fermented wheat bran shows an excellent feed replacement effect, and can partially replace corn and soybean meal, so that the feed digestibility is remarkably improved, and the feed cost is reduced. The invention provides a new way for high-value utilization of wheat bran, and has wide application prospect and important economic significance.

The invention provides a traditional Chinese medicine and probiotic composition for improving constipation and a preparation method thereof. The traditional Chinese medicine and probiotic composition for improving constipation comprises 10-20% of a probiotic composition, 15-25% of a traditional Chinese medicine composition and 55-75% of a carrier, the traditional Chinese medicine composition comprises lily, semen cassiae and mulberry; the probiotic composition is prepared from bifidobacterium breve, lactococcus lactis, lactobacillus acidophilus, lactobacillus plantarum, bifidobacterium bifidum, bifidobacterium longum and lactobacillus rhamnosus. According to the invention, traditional Chinese medicines are combined with various probiotics, and the various probiotics act together to help to recover intestinal microecological balance, promote nutrient absorption and improve lactose intolerance, so that the gastrointestinal function of a human body is comprehensively improved, and the problem of constipation is solved. The used traditional Chinese medicine components all belong to food which can be properly eaten, basically have no toxic or side effect, and can relax bowel and regulate spleen and stomach functions. The traditional Chinese medicines and various probiotics act together, so that the problem of constipation is fundamentally solved.

The present application relates to the technical field of food processing, and particularly relates to a processing method for cooked mutton, comprising the following steps: obtaining fresh mutton raw materials; performing pre-treatment before fermentation on the fresh mutton raw materials; adding a compound bacterial solution to the pre-treated fresh mutton raw materials and performing fermentation, so as to hydrolyze the amine substances in the mutton, and obtain fermented mutton; immersing the fermented mutton in a compound gel solution and performing vacuum impregnation, so as to selectively remove the free amine substances after fermentation, and obtain mutton with amine removed; after ingredient and curing treatment, the mutton with amine removed is cooked at 80 DEG C to 85 DEG C for 10 min to 15 min, and cooked mutton products are obtained; wherein the compound bacterial solution is composed of Staphylococcus carnosus, Pediococcus pentosaceus, Lactococcus lactis and calcium chloride solution. The present application degrades the branched short-chain fatty acids causing amine in mutton by the microbial amine reduction method, while the branched long-chain fatty acids with physiological activity are retained.

The invention relates to the technical field of synthetic biology and immunotherapy, in particular to an immune gene combination fragment responding to a tumor microenvironment, a recombinant plasmid, probiotics and application of the immune gene combination fragment, the recombinant plasmid and the probiotics. The preparation method comprises the following steps: selecting phytobacterium plantarum, casei casei, casei paracasei, streptococcus thermophilus and lactococcus lactis as a probiotic chassis, further designing and assembling an immune gene combination fragment of immune checkpoint inhibitors (PD-L1 and CTLA-4), and uploading the immune gene combination fragment to the bacterial strain to construct customized probiotics, so as to prepare the immune checkpoint inhibitors (PD-L1 and CTLA-4). The in-vitro expression condition is researched; it is found that in a mouse tumor model, the customized probiotics can release PD-L1 and CTLA-4 nano antibodies at the tumor site, the tumor microenvironment is reversed, the immune system is mobilized, and the anti-tumor effect is enhanced. The research is expected to provide a safe and efficient anti-tumor strategy for tumor treatment, and new hope is brought to tumor patients.

The invention relates to a microbubble drinking type tea yoghourt and a preparation method thereof, and the preparation method comprises the following steps: mixing tea juice, lemon juice, sodium citrate, raw milk and sugar, sterilizing, inoculating with a complex microbial inoculant for fermentation, and demulsifying to obtain the microbubble drinking type tea yoghourt, the strains in the complex microbial inoculant comprise a combination of streptococcus salivarius subsp. Thermophilus, lactococcus lactis subsp. Diacetyl, plant lactobacillus and leuconostoc mesenteroides subsp. Mesenteroides. In the invention, a complex microbial inoculant of four specific microbial strains is adopted to ferment the dairy product, and the four strains are further found to have a synergistic effect, so that the tissue state, the taste, the smell and the overall fusion effect of the tea yoghourt product prepared by final fermentation are further improved; the microbubble tea yoghourt beverage with excellent taste and stable tissue state is prepared.

The application discloses a recombinant lactococcus lactis oral vaccine, a preparation method and application thereof, characterized in that the recombinant lactococcus lactis expresses TcCTL-2 and / or TcCTL-5. The oral vaccine can not only effectively induce a mouse to produce a high-efficiency intestinal mucosal immune response, but also can stimulate the body to produce cellular immunity. The recombinant lactococcus lactis expressing TcCTL-2 and TcCTL-5 has the best protection effect on the mouse, and can be used as a preferred oral vaccine strain for preventing canine toxocariasis.

The present invention provides: an agent for reducing the concentration of a protein in blood, the agent containing Pediococcus acidilactici; and a food, a feed, and a medicine each of which is for reducing the concentration of a protein in blood and contains the agent for reducing the concentration of a protein in blood or Pediococcus acidilactici.

The invention belongs to the field of microorganisms, and particularly relates to screening of lactic acid bacteria capable of relieving ozostomia and application of oral cavity probiotic characteristics of the lactic acid bacteria. The invention provides pediococcus acidilactici ZJUIDS-C004, and the preservation number of the pediococcus acidilactici ZJUIDS-C004 is CGMCC (China General Microbiological Culture Collection Center) NO. 34145. The pediococcus acidilactici ZJUIDS-C004 can be used for inhibiting the growth of halitosis pathogenic bacteria with fusobacterium nucleatum (F.n), and the pediococcus acidilactici ZJUIDS-C004 has an inhibiting effect on a biological membrane formed by the halitosis pathogenic bacteria; the strain is used for solving oral health problems and relieving and improving halitosis.

The application belongs to the technical field of biology, and particularly relates to a recombinant strain of high-yield duck Riemerella anatipestifer phage lytic enzyme, a mutant strain, a construction method and application. The application provides a coding gene of duck Riemerella anatipestifer phage lytic enzyme, and further constructs a recombinant Lactococcus lactis of high-yield duck Riemerella anatipestifer phage lytic enzyme. The mutant strain provided by the application is obtained by using the recombinant Lactococcus lactis as a starting strain, through mutagenesis breeding technology and specific high-throughput screening strategies, has higher secretion expression of duck Riemerella anatipestifer phage lytic enzyme, and has good genetic stability in an antibiotic-free culture medium, and is suitable for industrialized and efficient production of active lytic enzyme. The duck Riemerella anatipestifer phage lytic enzyme expressed by the mutant strain has obvious lytic effect on duck Riemerella anatipestifer in vitro, has significant prevention and treatment effect on duck Riemerella anatipestifer infection in vivo, can effectively improve the survival rate of infected chicks, and can remove pathogenic bacteria in the body.