Micro-ecological preparation for feed and preparation method thereof

A technology of micro-ecological preparations and feeds, applied in animal feeds, animal feeds, applications, etc., can solve the problems that cannot satisfy the production and development of aquaculture, few types of biological feeds, and high prices, so as to reduce the rate of diarrhea, reduce the proportion of illnesses, alleviate The effect of the smell

Inactive Publication Date: 2010-08-25
韩建春
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to statistics, the country produces 2 million tons of various biological feeds annually. Compared with foreign countries, my country's biological feeds have fewer varieties, lowe

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The 5 strains of probiotics used in the present invention are all purchased from the China Industrial Microorganism Culture Preservation Management Center, which are: Lactobacillus plantarum CICC6051, Lactococcus lactis CICC6029, Lactobacillus rhamnosus CICC6141, Aspergillus niger CICC40700, Saccharomyces spp. CICC1717.

[0040] 1. Strain liquid fermentation process

[0041] 1.1 Activation of freeze-dried bacteria powder

[0042] Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacterial powders in ampoules of 5 strains into 0.9% In physiological saline, oscillate to make it dissolve, and activate it in a 30°C incubator for 30 minutes, and set aside.

[0043] 1.2 Expanded cultivation of liquid strains

[0044] 1.2.1 Preparation of primary seeds

[0045] Measure 200ml of culture medium for 5 strains into five 500ml Erlenmeyer flasks, sterilize at 121°C for 20 minutes and cool to 30°C, ...

Embodiment 2

[0053] 1. Strain liquid fermentation process

[0054] 1.1 Activation of freeze-dried bacteria powder

[0055]Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacteria powders in ampoules of 5 strains into 0.9% In normal saline, oscillate to make it dissolve, and activate it in a 30°C incubator for 30 minutes, and set aside.

[0056] 1.2 Expanded cultivation of liquid strains

[0057] 1.2.1 Preparation of primary seeds

[0058] Measure 200ml of culture medium for 5 strains into five 500ml Erlenmeyer flasks, sterilize at 121°C for 20 minutes and cool to 30°C, inoculate 10% of the medium volume with the activated strains in step 2.1, shake at 30°C The bed was cultivated for 24 hours, and the shaking speed was 120-130 rpm, and it was used as a primary seed.

[0059] 1.2.2 Preparation of secondary seeds

[0060] Measure 2000ml of culture medium for each of the five strains into five 500ml Erlenm...

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Abstract

The invention provides a micro-ecological preparation for a feed and a preparation method thereof. The method comprises the following steps of: firstly, performing liquid state fermentation on primary and secondary seeds by using 5 strains, namely lactobacillus plantarum, lactococcus lactis, lactobacillus rhamnosus, aspergillus niger and saccharomycopsis fibuligera; secondly, uniformly mixing high temperature soybean meal, wheat bran, corn meal and rice hulls in a weight ratio of (20-25):(50-60):(18-20):(3-5); adding 18 percent solution of urea in an amount which is 15 to 20 weight percent of the mixture into the mixture; inoculating liquid secondary seeds in an amount which is 5 percent of a humid weight of the solid mixture; uniformly mixing the mixture; flattening the solid mixture to a thickness of 12 to 20cm; culturing the solid mixture at the temperature of 30 DEG C for 48 hours; performing ventilation drying on the cultured solid mixture at the temperature of between 40 and 45 DEG C and stopping drying till the moisture content of the solid mixture is less than 5 percent; and crushing and packaging the solid mixture to obtain the finished products.

Description

(1) Technical field [0001] The invention relates to a biological preparation for feed, and also relates to a preparation method of the biological preparation. (2) Background technology [0002] The so-called feed microecological preparation is a safe, pollution-free and residue-free feed microbial preparation containing active probiotics, which is made by mixing known beneficial microorganisms and nutrient carriers through special processes such as fermentation and drying under the guidance of microecological theory. In 2000, the British "mad cow disease" incident and the Belgian "dioxin" incident occurred successively in the world, which caused panic among people all over the world and caused strong shocks in the European continent. Consumers' confidence in animal products was in a serious crisis. People began to strongly call for green feed, and put forward slogans such as "creating healthy food with healthy feed" and "feed is direct food for human beings". The safety of f...

Claims

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Application Information

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IPC IPC(8): A23K1/00
Inventor 韩建春陈成王宇程涛张英华
Owner 韩建春
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