Micro-ecological preparation for feed and preparation method thereof
A technology of micro-ecological preparations and feeds, applied in animal feeds, animal feeds, applications, etc., can solve the problems that cannot satisfy the production and development of aquaculture, few types of biological feeds, and high prices, so as to reduce the rate of diarrhea, reduce the proportion of illnesses, alleviate The effect of the smell
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Embodiment 1
[0039] The 5 strains of probiotics used in the present invention are all purchased from the China Industrial Microorganism Culture Preservation Management Center, which are: Lactobacillus plantarum CICC6051, Lactococcus lactis CICC6029, Lactobacillus rhamnosus CICC6141, Aspergillus niger CICC40700, Saccharomyces spp. CICC1717.
[0040] 1. Strain liquid fermentation process
[0041] 1.1 Activation of freeze-dried bacteria powder
[0042] Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacterial powders in ampoules of 5 strains into 0.9% In physiological saline, oscillate to make it dissolve, and activate it in a 30°C incubator for 30 minutes, and set aside.
[0043] 1.2 Expanded cultivation of liquid strains
[0044] 1.2.1 Preparation of primary seeds
[0045] Measure 200ml of culture medium for 5 strains into five 500ml Erlenmeyer flasks, sterilize at 121°C for 20 minutes and cool to 30°C, ...
Embodiment 2
[0053] 1. Strain liquid fermentation process
[0054] 1.1 Activation of freeze-dried bacteria powder
[0055]Measure 10ml of 0.9% normal saline into 5 test tubes, sterilize at 121°C for 20 minutes and cool to 30°C, pour all the freeze-dried bacteria powders in ampoules of 5 strains into 0.9% In normal saline, oscillate to make it dissolve, and activate it in a 30°C incubator for 30 minutes, and set aside.
[0056] 1.2 Expanded cultivation of liquid strains
[0057] 1.2.1 Preparation of primary seeds
[0058] Measure 200ml of culture medium for 5 strains into five 500ml Erlenmeyer flasks, sterilize at 121°C for 20 minutes and cool to 30°C, inoculate 10% of the medium volume with the activated strains in step 2.1, shake at 30°C The bed was cultivated for 24 hours, and the shaking speed was 120-130 rpm, and it was used as a primary seed.
[0059] 1.2.2 Preparation of secondary seeds
[0060] Measure 2000ml of culture medium for each of the five strains into five 500ml Erlenm...
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