658 results about "Saccharomyces" patented technology

The invention belongs to the field of biosynthesis, and in particular relates to LBD (Levandanenediol Pyrophosphate) synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, the sclareol biosynthesis yield is generally low, LSLPPs mutant containing at least one mutation of D730L, D374L, N674L or G379M is obtained by performing mutation and optimization on LSLPPs pyrophosphate synthase, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 26.11 g / L and is greatly improved compared with the prior art.

The invention belongs to the field of biosynthesis, and particularly relates to sclareol synthase and engineered yeast for producing sclareol. In order to solve the problem that in the prior art, sclareol biosynthesis yield is generally low, sclareol synthase is mutated and optimized, an SsScs mutant containing at least one mutation of Y491F, G307L or N269F is obtained, and the mutant is used for sclareol synthesis. Meanwhile, in order to solve the problem of genetic instability caused by free plasmid expression of part of saccharomyces cerevisiae strains, a sclareol synthesis pathway is constructed in a yeast genome, and meanwhile, an acetyl coenzyme A synthesis pathway, an MVA pathway and a sclareol synthesis pathway are enhanced; chassis bacteria for synthesizing sclareol are modified in a manner of knocking out part of transcription inhibition factors, and the yield of a 5L fermentation tank reaches 21.13 g / L and is greatly improved compared with the prior art.

The invention discloses an ecological planting method for remarkably improving the amino acid content of citrus, which comprises the following steps: (1) mixing and fermenting pruned citrus branches, weed straws, livestock and poultry manure and indigenous probiotic ferment powder to prepare a basic microbial fertilizer, and adding a calcium magnesium phosphate fertilizer and monopotassium phosphate to prepare a special microbial fertilizer for citrus, applying as a base fertilizer in winter; (2) after citrus pruning, in key phenological periods such as a flower bud period, a young fruit period and a fruit swelling period, an indigenous probiotic enzyme diluent with a specific dilution ratio is used for systematic foliage spraying on a tree body. The indigenous probiotic enzyme contains bacillus amyloliquefaciens, saccharomycetes, active enzymes and a plurality of nutrient elements. The method can significantly increase the total amino acid content of citrus fruits, greatly increase the yield, improve the taste of the fruits, enhance the tree body resistance, reduce the use of chemical pesticides, improve soil, and realize quality improvement, yield increase and green sustainable development of citrus planting.

The invention discloses an extraction method and application of lavender essential oil based on yeast fermentation pretreatment, and belongs to the technical field of plant active ingredient extraction. The method comprises the following steps: activating saccharomycetes, inoculating the saccharomycetes into a mixture containing a crushed lavender raw material and water, and fermenting at 30-38 DEG C for 20-28 hours to obtain fermentation liquor; then sterilizing and centrifuging the fermentation liquor to obtain supernate; and finally extracting the essential oil through steam distillation. Through yeast fermentation pretreatment, the lavender cell wall structure is effectively destroyed, essential oil release and active ingredient conversion are promoted, and the essential oil yield and antioxidant activity are remarkably improved. Compared with a traditional steam distillation method, the method has the advantages that the essential oil yield can be increased from 0.55% to 0.75% or above, and the DPPH free radical scavenging rate can be increased to 51.75% or above. The method is green in process and simple and convenient to operate, a new way is provided for high-value utilization of lavender resources, and the prepared essential oil can be used for developing cosmetics with anti-oxidation and anti-aging effects.

The invention discloses a compound rumen bypass guanidinoacetic acid additive as well as a preparation method and application thereof, and belongs to the technical field of animal feed additives. The compound rumen bypass guanidinoacetic acid additive provided by the invention is prepared from the following components in parts by mass: 40 to 60 parts of guanidinoacetic acid, 0.1 to 0.5 part of selenium yeast, 5 to 10 parts of nicotinamide, 5 to 12 parts of DL-methionine, 0.01 to 0.05 part of vitamin B12, 1 to 3 parts of compound probiotics, 3 to 5 parts of hydroxypropyl methyl cellulose, 1 to 2 parts of ethyl cellulose, 15 to 25 parts of hydrogenated palm oil and 3 to 5 parts of glyceryl monostearate. The compound probiotics comprise bacillus subtilis and saccharomyces cerevisiae. The compound additive provided by the invention can significantly improve the daily gain of ruminants and reduce the feed-gain ratio, and meanwhile, due to the improvement of the energy utilization efficiency and the enhancement of the antioxidant ability, the immunity of animals is improved, the morbidity is reduced, and the meat quality is improved.

The invention discloses a scalp care solution with the effects of controlling oil, relieving, inhibiting bacteria and adjusting scalp micro-ecology and a preparation method and application thereof.The preparation method of the scalp care solution comprises the following steps that a fermentation substrate is inoculated with saccharomycetes, primary fermentation culture is conducted for 24-72 h, then secondary fermentation culture is conducted through Lactobacillus gasseri, and the scalp care solution is obtained; wherein the fermentation substrate comprises rice, barley seeds and water; wherein the saccharomycetes comprise Saccharomyces cerevisiae (Saccharomyces cerevisiae) and Saccharomyces fibulligera (Saccharomyces fibulligera), and the saccharomycetes comprise Saccharomyces cerevisiae (Saccharomyces fibulligera) and Saccharomyces fibulligera (Saccharomyces fibulligera). The rice and the barley seeds are fermented in a microbial fermentation mode, the bioavailability of the rice and the barley seeds is improved, the content of active ingredients in the fermentation liquor prepared through the fermentation technology is increased, the fermentation liquor has the ideal effects of controlling oil, resisting inflammation, inhibiting bacteria, adjusting scalp micro-ecology and the like, the preparation technology is simple, and the method is suitable for industrial production. The fermentation conditions are mild, no organic solvent is used, and safety is achieved.

The invention provides Saccharomyces cerevisiae for producing squalene as well as a screening method and application of the Saccharomyces cerevisiae. The method comprises the following steps: culturing a Saccharomyces cerevisiae engineering strain ySC782 for synthesizing squalene to obtain a to-be-mutagenized bacterial solution, carrying out ARTP mutagenesis on the to-be-mutagenized bacterial solution, adding the to-be-mutagenized bacterial solution into a YPD liquid culture medium, culturing to obtain a resuscitation bacterial solution, adding the resuscitation bacterial solution into a sucrose solution with gradient concentration distribution, screening, and repeating the ARTP mutagenesis-sucrose solution screening step to obtain the squalene mutant strain. And finally, the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening, and the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening. The yield of squalene produced by the saccharomyces cerevisiae ySC782-M5 reaches 49.83 g / L, so that a solution is provided for breaking through the yield bottleneck of squalene synthesized by yeast.

The invention relates to the technical field of microorganisms, in particular to a compound probiotic agent for reducing harmful gases generated in intestinal tracts and regulating intestinal flora. The compound probiotics disclosed by the invention are prepared from bifidobacterium animalis subsp. Lactis Bi66, plant lactobacillus P16 and a saccharomyces boulardii Bld-3 strain. The three strains are compounded and combined for use, so that the content of short-chain fatty acid in intestinal flora can be remarkably increased, the yield of beneficial gases such as CH4 and H2 can be increased, the yield of NH3 and H2S which are harmful to a human body can be reduced, beneficial bacteria can be promoted, and the growth of harmful bacteria can be inhibited, so that the intestinal flora can be regulated. Under the condition of keeping the same amount of the used bacteria, compared with a strain intervention mode without any bacteria, the compounding of the three bacteria has the best effect in the aspect of improving the intestinal flora, which indicates that the three bacteria can be mutually matched and promoted, and can have synergistic interaction in the aspect of regulating the intestinal flora.

The invention discloses saccharomyces boulardii, a culture and a preparation method and application of the saccharomyces boulardii. The preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 20252633. The preparation method comprises the following steps: preparing saccharified liquid by taking white spirit vinasse or brewer's grains as a main raw material and adopting a high-temperature alkali treatment and composite enzymolysis process, inoculating strains for liquid-state high-density fermentation, carrying out solid-liquid separation on fermentation liquor, taking a liquid phase, and carrying out spray drying. According to the method, high-value utilization of the waste vinasse is realized, and the production cost is remarkably reduced. The obtained culture is rich in viable bacteria and active small peptides, and has the characteristics of strong tolerance, wide antibacterial spectrum and the like. As a feed additive, the feed additive can effectively reduce the diarrhea rate of piglets, inhibit clostridium perfringens and prawn vibrios of broilers, and improve the oxidation resistance and production performance of animals.

The invention discloses a composition containing saccharomycetes / rice fermentation product filtrate, which has the effects of controlling oil and repairing hair. The composition comprises saccharomycetes / rice fermentation product filtrate, a rice husk extract, a tuber melanosporum extract and a millet seed extract. The invention also provides an application of the composition in preparation of oil-controlling, repairing and washing products, shampoo containing the composition and a preparation method of the shampoo. Experimental results show that the composition can significantly inhibit grease secretion of human sebaceous gland cells, repair hair and improve smoothness and glossiness of hair. The composition disclosed by the invention has the advantages of strong synergistic effect of all components, safety, no irritation, simple preparation process and suitability for industrial production.

The invention discloses a genetically engineered bacterium and an application of the genetically engineered bacterium in production of farnesene. The genetically engineered bacterium takes saccharomyces cerevisiae as a chassis strain; an exogenous beta-farnesene synthase gene is integrated, an ERG9 gene is knocked out, a promoter of a truncated HMG1 gene is replaced with an ANB1 promoter, an exogenous transhydrogenase gene is integrated and driven by a glucose sensitive promoter, beta-farnesene synthase is mutated and fused with a transmembrane structural domain of ABC transporter protein PDR10, and then a chimeric expression unit is constructed. According to the present invention, the chassis bacteria are modified by at least one of the steps of overexpression of phosphatidylcholine synthetase, knockout of sterol ester synthetase and introduction of exogenous alkyl glycerol monooxygenase, and the obtained engineering bacteria are combined with the optimized fermentation process so as to significantly improve the farnesene synthesis efficiency and the production economy; therefore, the genetically engineered bacterium and the fermentation method provided by the invention have good application prospects.

The invention provides yeast HAU2501 and application thereof in preparation of fermented vegetables, and belongs to the technical field of fermentation microorganisms. The invention discloses saccharomycetes HAU2501, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is July 21, 2025, and the preservation number is CGMCC No.35320. The saccharomycetes HAU2501 has the advantages that the saccharomycetes HAU2501 can be used for preparing the saccharomycetes HAU2501; the saccharomycetes HAU2501 can be used as an excellent aroma-producing leavening agent, can effectively improve the flavor quality of the fermented vegetables through synergistic fermentation with lactic acid bacteria, and provides a theoretical basis and a technical reference for flavor regulation and control of traditional fermented vegetables and development of functional leavening agents.

The invention belongs to the technical field of enzyme mutants, and discloses a mutant of lycopene cyclase and application of the mutant, glutamic acid at the 321 site of wild type lycopene cyclase which is derived from arabidopsis and has an amino acid sequence as shown in SEQ ID NO.1 is mutated into lysine, phenylalanine at the 319 site of the wild type lycopene cyclase is mutated into leucine, cysteine at the 323 site of the wild type lycopene cyclase is mutated into alanine, and the mutant of the lycopene cyclase is obtained. 1, and the lycopene cyclase mutant with the amino acid sequence as shown in SEQ ID NO. 2 is obtained. BTS1, CrtB and CrtI are integrated into a saccharomyces cerevisiae BY4741 strain, and a chassis strain ZA1 for stably producing alpha-carotene precursor lycopene is constructed; mutant expression plasmids are constructed, positive clone strains are screened out, and the epsilon-cyclization ability of mutant enzymes is remarkably higher than the beta-cyclization ability; the method comprises the following steps: by taking lycopene as a substrate, constructing an expression vector lipid droplet surrounding protein PET10; gene PAH1, DGA1 and Cat2 related to TAG synthesis and perilipid droplet protein PET10 are constructed on an expression vector and converted into ZA1, and when lycopene is used as a substrate, the efficiency of catalytic production of alpha-carotene is improved.

The invention provides a preparation method of a fruit and carrot fermented beverage, and relates to the technical field of biological fermentation preparation. The preparation method of the fruit carrot fermented beverage comprises the following steps: selecting fresh fruit carrots, washing, steaming, pulping, filtering and sterilizing to obtain filtrate; adjusting the temperature to 30-40 DEG C, inoculating lactobacillus fermentum into the filtrate, performing anaerobic fermentation for 3-6 hours to obtain a fermentation product A, adjusting the temperature to 28-30 DEG C, inoculating saccharomyces cerevisiae into the fermentation product A, and performing anaerobic mixed fermentation for 4-9 hours to obtain a fermentation product B; according to the invention, lactobacillus fermentum is used for pre-fermentation of carrots, and mixed fermentation is carried out in combination with intervention of later-stage saccharomyces cerevisiae, so that olefinic terpene substances in the carrots are effectively reduced, the earthy smell in the carrot fermented beverage is improved, and the color, luster and fragrance can be improved on the basis of stably maintaining the clarity of the existing carrot fermented beverage; the comprehensive competitiveness of the carrot fermented beverage in the market is improved.

The invention discloses a yeast bionic BiVO4 composite photocatalytic material and a synthesis method and application thereof.The synthesis method comprises the steps that firstly, bismuth nitrate pentahydrate, sodium dodecyl benzene sulfonate, nitric acid, ammonium metavanadate and sodium hydroxide serve as raw materials, yeast is added at the same time, and a yeast / BiVO4 composite photocatalyst is prepared through a hydrothermal reaction; and the photocatalyst is used for photocatalytic degradation of 2, 4-dichlorophenol. A hydrothermal reaction method is used, and the synthesis process is simple; the yeast / BiVO4 composite material is of a spherical structure, and compared with a traditional nano-powder or random-form catalyst, the three-dimensional spherical photocatalyst has the main advantages that the three-dimensional spherical photocatalyst is derived from a unique physical structure and can bring chemical property improvement, so that the efficiency of photocatalytic degradation of 2, 4-dichlorophenol is improved.

This invention relates to the Kluyveromyces marxianus strain and its use, wherein the Kluyveromyces marxianus strain is Kluyveromyces marxianus AMCC30648, deposited with the China Center for the Preservation of Typical Cultures (CCTCC) with deposit number CCTCC NO:M 20222108, or Kluyveromyces marxianus AMCC31343, deposited with the China Center for the Preservation of Typical Cultures (CCTCC) with deposit number CCTCC NO:M 20222111. The Kluyveromyces marxianus strain is characterized by producing a variety of flavor substances at high concentrations. It can be used in fields such as brewing, beverages, and bakeries.

The invention relates to the technical field of animal feed, and particularly discloses a bamboo powder dietary fiber probiotic feed additive as well as a preparation method and application thereof. The additive consists of bamboo powder dietary fibers, a probiotic agent and a carrier, wherein probiotics are at least one of trichoderma, bacillus, lactic acid bacteria and saccharomycetes. The preparation method comprises the steps of bamboo powder pretreatment, solid state fermentation, drying, mixing and the like. The product fully utilizes the prebiotics characteristics of the bamboo powder and the regulation effect of probiotics, can effectively improve the intestinal health of livestock and poultry, improve the feed utilization rate and reduce the breeding cost, can partially or completely replace antibiotics, and has the characteristics of greenness, safety and high efficiency.

The invention discloses a method for retaining flavor of plum-leaf crab ice wine. The method comprises the following steps: 1, selecting red-pulp malus spectabilis fruits and eight-edge malus spectabilis fruits, washing, and draining with a vibrating screen until the surface moisture is less than or equal to 5%; 2, denucleating, taking pulp, pulping and sieving to obtain plum-leaf crab normal juice; 3, beta-glucosidase is added; 4, adding modified pectin microspheres, and carrying out three-stage cooling to obtain frozen plum-leaf crab normal juice; 5, slowly unfreezing, inoculating low-temperature-resistant saccharomyces cerevisiae liquid, and carrying out low-temperature segmented fermentation; 6, ageing; and 7, adding potassium tartrate, continuing for 2-3 months, filtering, and clarifying until the turbidity is less than or equal to 2NTU. The modified pectin microspheres with the adsorption-slow release function are added, terpene and ester flavor precursors in the plum-leaf crab are adsorbed in the fermentation period, the volatilization and hydrolysis rate of flavor substances in the fermentation process is reduced, especially the retention rate of plum-leaf crab characteristic fruity substances such as ethyl caprylate and ethyl caprate can be increased, and after being degraded, the modified pectin microspheres can be used for degrading the plum-leaf crab characteristic fruity substances. And the components are released back into the wine body.

The invention relates to a Saccharomyces cerevisiae C16I09 strain which is preserved in the China Center for Type Culture Collection on May 21, 2025, and the preservation number of the Saccharomyces cerevisiae C16I09 strain is CCTCC (China Center for Type Culture Collection) No. M20251150. The strain disclosed by the invention has good oxidation resistance and can be applied to the fields of skin care products, health care products, anti-aging foods and the like. Especially, extracellular fermentation broth of the strain, namely a fermentation product or a fermentation extract of the strain, has excellent oxidation resistance, can scavenge free radicals and relieve oxidative damage, has the effects of protecting skin, resisting inflammation, delaying senescence and the like, and can be applied to the fields of skin care products, health care products, anti-aging foods and the like as a natural anti-aging agent.

This invention discloses a processing technology for antibody-enhancing probiotic feed, which includes raw material pretreatment, substrate preparation, sterilization, cooling, strain activation, aseptic inoculation, constant-temperature anaerobic fermentation, immune-enhancing compounding, filtration and homogenization, and aseptic filling and storage. The fermentation substrate liquid includes purified water, molasses, yeast extract, compound trace elements, and buffer salts. A compound microbial community composed of *Lactobacillus plantarum*, *Bacillus subtilis*, *Bifidobacterium*, and yeast is used for fermentation. The temperature is controlled at 35–39°C during fermentation, and intermittent stirring is employed. After fermentation, astragalus polysaccharide, echinacea extract, and trehalose are added for compounding. This invention reduces the loss of active ingredients by adding immune-enhancing components after fermentation, and improves the survival rate of probiotics and fermentation stability through intermittent stirring. The resulting liquid feed has a high live bacteria content and immune-enhancing effect.

The invention discloses a preparation method and application of a tetraploid saccharomyces cerevisiae strain, and belongs to the technical field of bioengineering. Comprising the following steps: (1) knocking out an HO gene of diploid yeast LFN524, separating haploids through spore production, and identifying mating types of candidate haploid strains to obtain haploid strains with opposite mating types of haploids; (2) respectively culturing haploid strains of different mating types, inducing fusion of the haploid strains, and screening successfully fused diploid strains; and (3) inducing the successfully fused diploid strain to fuse again to obtain the tetraploid saccharomyces cerevisiae strain. The nucleic acid content of the strains 8-3 and 8-4 disclosed by the invention is obviously higher than that of a diploid stock plant, and the nucleic acid content can respectively reach 0.118 g / g-DCW and 0.096 g / g-DCW, which are respectively increased by 63.89% and 33.33%.

The invention discloses debaryomyces hansenii with high yield of umami peptide and application of the debaryomyces hansenii in improving the umami of low-salt fermented sausages, the debaryomyces hansenii is separated from the anglerkang sausages, is preserved in the China General Microbiological Culture Collection Center on September 30, 2025, and has the preservation number of CGMCC (China General Microbiological Culture Collection Center), and the preservation number of the debaryomyces hansenii HYYAKS044 is CGMCC NO. The biological preservation number is CGMCC (China General Microbiological Culture Collection Center) The content of peptides below 3kDa of the sausage inoculated with the debaryomyces hansenii disclosed by the invention is obviously more than that of a non-inoculated group, and six umami peptides, namely DDHFLFDKP, DPTPSDGKL, FPPDVGGN, ISEQFTAMFR, PETPPP and VDNPGHPFI, can be screened from the peptides below 3kDa, and the six umami peptides are respectively DDHFLFDKP, DPTPSDGKL, FPPDVGGN, ISEQFTAMFR, PETPPP and VDNPGHPFI.

The invention relates to a rhodotorula mucilaginosa strain and a yellow wine co-fermentation process thereof, and belongs to the technical field of fermentation engineering and strains. The invention provides the rhodotorula mucilaginosa with the fermentation characteristics of low temperature resistance, acid resistance, high yield of beta-glucosidase and aroma production, sequential inoculation is adopted, the rhodotorula mucilaginosa H04 is firstly inoculated for fermentation for two days, then the saccharomyces cerevisiae is inoculated, and the yellow wine co-fermentation is carried out by an equal-proportion 20 DEG C low-temperature fermentation process. The yellow rice wine meeting national standard requirements of yellow rice wine can be obtained after 20 days of fermentation, and the volatile flavor type, content, taste and aroma sensory quality of the yellow rice wine after fermentation are improved.

The application discloses a method for continuously fermenting urine to produce safe fertilizer, which comprises the following steps: in the first fermentation, adding environment-friendly enzyme into a container, then collecting fresh urine and adding molasses, and carrying out fermentation in a sealed manner; after the fermentation is completed, reserving part of the fermentation liquor as a substrate; and in the subsequent batches, directly adding fresh urine and molasses into the container with the substrate to carry out fermentation, so as to realize continuous circulation. The method can inhibit the hydrolysis process of urea in the urine in the early stage by timely adding environment-friendly enzyme (or the fermentation liquor of the last time) and molasses into the fresh urine to carry out fermentation, thereby greatly reducing the ammonia nitrogen volatilization amount, and the pathogenic bacteria in the urine can be inhibited to a very low level in a short period of time. Except that the environment-friendly enzyme needs to be added in the first fermentation, the environment-friendly enzyme does not need to be added in the subsequent batches of fermentation, and only a small amount of molasses needs to be added. A large amount of beneficial bacteria such as lactic acid bacteria and yeast and metabolic products such as lactic acid and acetic acid are generated in the fermentation process, so that the urine is converted into microbial bacterial fertilizer.

The application provides a pediococcus acidilactici M4 for low-moisture fermented feed and an application thereof, and belongs to the field of microbial application and feed technology. The application uses the pediococcus acidilactici M4 to prepare fermented total mixed ration. The bacteria have the characteristics of high osmosis and fast acid production, and can reduce the pH value of the fermentation system in a short time; have strong broad-spectrum bacteriostatic effect on escherichia coli, chicken white dysentery salmonella and staphylococcus aureus; and perfectly adapt to the fermentation of low-moisture total mixed ration. The strain is used as the fermentation strain of low-moisture total mixed ration, can significantly reduce the content of acid detergent fiber and neutral detergent fiber, improve the crude protein level, and then improve the digestibility of the daily ration, increase the content of probiotics such as lactic acid bacteria and yeast in the daily ration, reduce the content of pathogenic bacteria such as escherichia coli in the daily ration, and also can improve the content of volatile fatty acid in the daily ration, improve the quality of the daily ration, and improve the feeding value of the daily ration.

The invention relates to the technical field of biological breeding, and discloses a composite microecological preparation for adjusting lamb intestinal flora and relieving weaning stress, the composite microecological preparation comprises, by weight, 10-40 parts of enterococcus faecalis powder, 10-40 parts of bacillus subtilis powder and 10-40 parts of saccharomycetes powder, the enterococcus faecalis powder is prepared through a gradient acid-resistant pre-domestication system, and the bacillus subtilis powder is prepared through a gradient acid-resistant pre-domestication system. The bacillus subtilis powder is prepared through an oxygen-controlled induced spore strengthening process, the saccharomycetes powder is prepared through a beta-glucan strengthening reactor, and the three kinds of bacterium powder are mixed under the sterile condition to obtain the bacillus subtilis powder, the saccharomycetes powder and the beta-glucan strengthening reactor. The tolerance, activity and stability of the strain are improved, the controllable and repeatable preparation process of the compound microecological preparation is ensured, and a reliable technical basis is provided for regulating intestinal flora of weaned lambs and relieving weaning stress.

The invention provides a yeast rich in polyphosphate and application of the yeast in synthesis of adenosine triphosphate, and the content of polyphosphate is 15-35 wt% based on the dry matter mass of the yeast rich in polyphosphate; according to the method disclosed by the invention, the yeast is subjected to phosphorus starvation culture and then is subjected to phosphorus supplementary culture, so that the yeast is enriched with polyphosphates, and the yeast rich in polyphosphates is obtained; then, the yeast rich in polyphosphate is used as a biocatalyst to synthesize ATP, that is, self-related enzyme systems, mainly including adenosine kinase, endo-polyphosphate kinase and excision-polyphosphate kinase, in the yeast rich in polyphosphate are used for replacing exogenous polyphosphate kinase and adenosine kinase, and in addition, ATP is synthesized by using the yeast rich in polyphosphate as a biocatalyst. According to the method for synthesizing ATP by using the yeast rich in polyphosphate as the biocatalyst, exogenous adenosine kinase and exogenous polyphosphate kinase do not need to be additionally added, the cost is low, the efficiency of converting adenosine into ATP is high, the batch-to-batch stability of the yeast is realized, and the enzyme activity of the self-related enzyme system is high.

The invention provides hansenula polymorpha YC-C-16 capable of producing lactic acid and application of the hansenula polymorpha YC-C-16, and belongs to the technical field of microorganisms and wine brewing. The hansenula polymorpha YC-C-16 is characterized in that the hansenula polymorpha YC-C-16 is named as hanseniaspora uvarum in taxonomy, is preserved in the China Center for Type Culture Collection, has the preservation number of CTCC M 20252302, is preserved in Wuhan University on October 22, 2025, and is named as hanseniaspora uvarum in Wuhan University. The grape juice hansenula polymorpha YC-C-16 disclosed by the invention can be used for fermenting grape wine independently or together with saccharomyces cerevisiae, the content of lactic acid in the grape wine can be increased, and flavor substances such as ethyl butyrate, alpha-terpilenol, ethyl salicylate, ethyl myristate and trans-2-heptenal are added, so that the flavor level of the grape wine is increased. The hansenula polymorpha YC-C-16 provided by the invention can be widely applied to the field of wine fermentation.

The invention relates to a recombinant genetically engineered bacterium for synthesizing terpenoids. The recombinant genetically engineered bacterium is saccharomyces cerevisiae in which a gal80 gene, an lpp1 gene and a dpp1 gene are knocked out and a heterologous MVA pathway gene and an AuoblA gene are integrated. According to the invention, a ophiobollin synthesis route is introduced into the saccharomyces cerevisiae in a heterologous manner for the first time, so that de novo synthesis of a ophiobollin skeleton compound, namely the ophiobollin F and an oxidation product of the ophiobollin F is realized; the synthesis of the ophiobolin F of 5.1 g / L is realized, which is also the highest sesterterpene heterologous synthesis yield reported at present; according to the invention, the ophiobolin 5-OH-21-CHO-opF with a new structure, which has excellent anti-cancer activity, is obtained through identification; the engineering strain of high-yield ophiobolin U is constructed, and the shake flask yield reaches 128.9 mg / L.

The invention discloses ganoderic acid mass production and application based on cytochrome P450 enzyme CYP512W6, the cytochrome P450 enzyme CYP512W6 in ganoderma lucidum is converted into a corresponding saccharomyces cerevisiae strain for heterologous expression, and three kinds of ganoderic acid, namely TLTOA, 3 beta-TLTOA and DLDOA, are obtained after fermentation. The invention provides a new implementation mode for heterologous biosynthesis of ganoderic acid in saccharomyces cerevisiae.