5108 results about "Saccharomyces" patented technology

The curative action of drugs, including herbal remedies, allopathic remedies, and periodontal remedies, is enhanced and accelerated by administering such drugs in combination or association with probiotics, especially those of genus Lactococcus, Lactobacillus, Pediococcus, Streptococcus, Propionibacterium, Brevibacterium, Penicillium, and Saccharomyces.

The present invention is directed to telomerase nucleic acids and amino acids. In particular, the present invention is directed to nucleic acid and amino acid sequences encoding various telomerase protein subunits and motifs, including the 123 kDa and 43 kDa telomerase protein subunits of Euplotes aediculatus, and related sequences from Schizosaccharomyces, Saccharomyces sequences, and human telomerase. The present invention is also directed to polypeptides comprising these telomerase protein subunits, as well as functional polypeptides and ribonucleoproteins that contain these subunits.

The present invention is directed to novel telomerase nucleic acids and amino acids. In particular, the present invention is directed to nucleic acid and amino acid sequences encoding various telomerase protein subunits and motifs, including the 123 kDa and 43 kDa telomerase protein subunits of Euplotes aediculatus, and related sequences from Schizosaccharomyces, Saccharomyces sequences, and human telomerase. The present invention is also directed to polypeptides comprising these telomerase protein subunits, as well as functional polypeptides and ribonucleoproteins that contain these subunits.

The present invention discloses a natural fruit and vegetable ferment beverage, and a preparation method thereof. The preparation method for the ferment beverage comprises: adding freshly-squeezed fruit and vegetable juice to marine fish skin collagen peptide; adopting the resulting mixture as a fermentation substrate; carrying out dilution, slurry mixing and sterilization, and then adopting a direct vat and freeze-dried type lactic acid starter to carry out fermentation; stopping the fermentation after 7-11 days until the pH value is 4.3; adding a supplementary feed and carrying out sterilization; then adding high-activity dried yeast to carry out fermentation; stopping the fermentation after 5-7 days until the pH value is 4.0; after storing the fermentation broth at the low temperature, carrying out homogenization, concentration, centrifugation, blending, sterilization and bulking to finally prepare the novel natural fruit and vegetable ferment beverage. According to the ferment beverage of the present invention, the stepwise fermentation is adopted for the freshly-squeezed fruit and vegetable juice by using the lactic acid bacteria and the yeast; the marine fish skin collagen peptide is added to promote the growth and the metabolism of fermentation bacteria, such that metabolic products of nutritional ingredients in the natural fruits and vegetables are rich so as to provide a beneficial nutrition and health effect.

The invention discloses a compound microbial culture starter and application thereof, which belong to an application of a beneficial microbial preparation at low cost. The active microorganism of the compound microbial culture starter comprises saccharmyces cerevisiae, bacillus subtilis, bacillus natto and lactococcus lactis. The compound microbial culture starter can be used for livestock and poultry raising beds, feeds addictive and other aspects and is characterized by reducing the cost input of users on the culture starter and having stable effect.

There is disclosed a method of producing isobutanol. In an embodiment, the method includes providing a microorganism transformed with an isobutanol producing pathway containing at least one exogenous gene. The microorganism is selected to produce isobutanol from a carbon source at a yield of at least 10 percent theoretical. The method includes cultivating the microorganism in a culture medium containing a feedstock providing the carbon source, until isobutanol is produced. The method includes recovering the isobutanol. In one embodiment, the microorganism is a yeast with a Crabtree-negative phenotype. In another embodiment, the microorganism is a yeast microorganism with a Crabtree-positive phenotype. There is disclosed a microorganism for producing isobutanol. In an embodiment, the microorganism includes an isobutanol producing pathway containing at least one exogenous gene, and is selected to produce a recoverable quantity of isobutanol from a carbon source at a yield of at least 10 percent theoretical.

A biologic deodoring and cleaning agent for treating the refuse to prepare fertilizer and cleaning and activating water area contains high-safety yeast, lactobacillus, nitrogen-fixing bacteria, functional bacillus, and photosynthetic bacteria.

The invention relates to a kitchen waste recycling automatic treating all-in-one machine and a treatment process thereof. The all-in-one machine comprises a crushing device, a squeezing device, an oil-water separating device and a biological treating device, wherein the crushing device is used for crushing kitchen waste into residue bodies with the food particle size of 0.1 to 0.3 millimeters; liquid in solid residues squeezed by the squeezing device accounts for 7 to 15 percent of the total weight of solid residues; the solid residues enter the biological treating device for treatment finally, and a microbial agent is put into a fermentation tank in the biological treating device, heated and subjected to batch-type stirring; the microbial agent consists of actinomycetes, lactobacillus, saccharomycetes and photosynthetic bacteria and are fed in a mass ratio of 3:5:7:4; 0.01 to 2 mass parts of the microbial agent are added into 100 mass parts of solid residues; and the fermentation time of the microbial agent is 3 to 15 hours. The integrated equipment is small in size, compost time can be shortened greatly, and the treatment efficiency is improved.

The invention discloses rice wine yeast and a preparation method thereof. The yeast comprises the following materials in part by weight: 60 to 100 parts of sticky rice, 1 to 10 parts of licorice root, 1 to 10 parts of dried orange peel, 1 to 10 parts of cyperus rotundus, 1 to 10 parts of polygonum flaccidum meism, 1 to 10 parts of mulberry leaves, 1 to 10 parts of stringy stonecrop and 0 to 15 parts of water. Compared with the prior art, five Chinese medicaments such the licorice root, the dried orange peel, the cyperus rotundus, the polygonum flaccidum meism and the mulberry leaves are selected and added into the yeast of the invention, which not only prompts the growth and the metabolism of the yeast, but also inhibits the growth of pernicious bacteria; simultaneously the flavor of the yeast is improved by adding sweet-scented osmanthus; and simultaneously rice wine produced by using the yeast has a unique flavor of the sweet-scented osmanthus. The stringy stonecrop serving as a Chinese medicament is added into the yeast, which not only makes the yeast contain active ingredients, for protecting liver, in the stringy stonecrop, but also makes the rice wine produced by using the yeast have the efficacy of protecting the liver.

A gene activation / inactivation and site-specific integration system has been developed for mammalian cells. The invention system is based on the recombination of transfected sequences by FLP, a recombinase derived from Saccharomyces. In several cell lines, FLP has been shown to rapidly and precisely recombine copies of its specific target sequence. For example, a chromosomally integrated, silent β-galactosidase reporter gene was activated for expression by FLP-mediated removal of intervening sequences to generate clones of marked cells. Alternatively, the reverse reaction can be used to target transfected DNA to specific chromosomal sites. These results demonstrate that FLP can be used, for example, to mosaically activate or inactivate transgenes for a variety of therapeutic purposes, as well as for analysis of vertebrate development. The FLP recombination system of the present invention can be incorporated in transgenic, non-human mammals to achieve site-specific integration of transgenes, to construct functional genes or to disrupt existing genes.

The invention relates to a micro-ecological preparation and the application thereof, in particular to a micro-ecological preparation containing a variety of probiotics and the application thereof. The micro-ecological preparation contains any three or four of the components including CGMCC No. 2383 bacillus licheniformis powder, bacillus subtilis powder, CGMCC No. 2386 enterococcus faecalis powder, lactobacillus acidophilus powder, and CGMCC No. 2388 saccharomyces cerevisiae. The micro-ecological preparation has content of live bacteria, has the adversity resistance such as gastric acid resistance, bile salt resistance, high-temperature resistance, common antibiotic resistance, and the like and the probiotic functions of producing acid and enzyme and resisting pathogenic bacteria. The variety and the proportion of the probiotics and carrier can be determined according to different kinds of animals and different animal growth phase. The micro-ecological preparation can improve the feed utilization efficiency, increase the yield of meat, eggs and milk, promote the growth of the animal, improve the immunity and the disease resistance of the animal, replace the antibiotic and improve the quality of the animal product.

The invention discloses a composite microorganism viable bacteria preparation, which contains pediococcus, yeast and bacillus with the total bacteria count of more than 1 billion per gram. The invention also discloses a method for preparing the composite microorganism viable bacteria preparation, which comprises the following steps: performing amplification liquid culture on various strains respectively according to a conventional method in the field; mixing the materials according to a required proportion; and performing solid fermentation, drying and pulverization to obtain the composite microorganism viable bacteria preparation. The invention also discloses application of the composite microorganism viable bacteria preparation in preparing an organic waste water purifying agent and an aquaculture water body purifying agent, a deodorizer, a degreasing agent or an agent for removing pipe blockages for the hygiene of a refuse tip, a public place, an agricultural product market or a kitchen, feed additives in poultry and animal husbandry or pet culture, and preparations for controlling agricultural microorganism diseases. The composite microorganism viable bacteria preparation can effectively decompose a large amount of putrescent organic matters, inhibit the growth of harmful microorganisms and eliminate peculiar smells, and has the characteristics of high ammonia nitrogen removal rate, no generation of residual sludge, effective deodorization, low cost and the like.

The invention relates to a production process for vinegar, in particular to the standardized and industrialized production process for Shanxi mature vinegar, which solves the problems of long production process period, complex process, unstable product quality, low raw material utilization rate and the like, of the prior production process. The production process comprises the following steps of: performing material processing before alcoholic fermentation, crushing, mixing, liquefaction and saccharification; selecting high-temperature resistant and high-alcoholic strength resistant saccharomyces, and adding a composite yeast to perform alcoholic fermentation; adding the composite acetic bacteria into vinegar residue, stirring with a vinegar residue stirrer, fermenting the vinegar residue in an acetyl hydrate fermenting tank, and turning the vinegar residue with a vinegar residue turning machine; tamping the vinegar residue in the acetyl hydrate fermenting tank, covering a layer of table salt on the tamped vinegar residue, and standing the vinegar residue; fuming the vinegar residue by the combination of a vinegar residue fuming furnace and a steam vinegar residue fuming jar; pouring vinegar; ageing the vinegar; clarifying the vinegar with a crude acidic protease, and filtering the vinegar with kieselguhr; blending the vinegar and the traditional Shanxi mature vinegar; sterilizing the vinegar; and packing the vinegar. The process of the invention has the advantages of systematized flow, standardized technological parameters, scale production procedure, normalized product quality and optimized production process.

The invention provides a natural fruit and vegetable enzyme beverage blended thick slurry and a production method thereof. One or more kinds of fruits and vegetables are squeezed into juice, the separated fruit and vegetable residue and peels, seeds and pulps of the fruits and vegetables are added with sugar and are fermented and proliferated by yeasts to form yeast cells, so that yeast polypeptide amino acid is prepared; fruit and vegetable juice is added with saccharose and the yeast polypeptide amino acid and then is subjected to synchronous fermentation of yeasts and lactic acid bacteria; fermentation liquid is added with saccharose, high fructose corn syrup and soaked white fungus, the mixture is ground, homogenized, heated and sterilized, and then is subjected to hot filling and is sealed, wherein the shelf life is more than one year, the terminal product is not added with any food additive and is not added with nutrient supplements artificially, and is a pure natural heath drinking. The yeasts and the lactic acid bacteria are utilized for fermenting fruits and vegetables, a number of enzyme metabolites are produced and accumulated, and compared with nutrition and functional components contained in fruit and vegetable raw materials and microorganisms, the natural fruit and vegetable enzyme beverage blended thick slurry has beneficial efficacies of promoting digestion, enhancing immunity, delaying senescence of a human body and the like.

The invention discloses a novel compound microorganism living bacteria agent. The agent comprises probiotics such as pediococcus, saccharomyces, bacillus and the like, wherein the total bacterial quantity is above 107 colony-forming units (cfu) / gram. The invention further discloses a method for preparing the novel compound microorganism living bacteria agent and applications of the novel compound microorganism living bacteria agent in preparation of organic sewage purification additives, deodorization fly dispelling agents, degreasing agents, pipeline blockage removing agents, feed additives and microorganism disease prevention agents. The novel compound microorganism living bacteria agent has the comprehensive effects of high removal rates to ammonia nitrogen, total nitrogen and total phosphorus, small sludge residues, energy saving, environmental friendliness and the like and the characteristics of convenience in use, safety and quickness in function, no secondary pollution, benefits to ecological protection, low treatment cost, good comprehensive profit and the like.

The invention relates to a method for producing a high-efficiency composite microbial agent by using livestock liquid dung, and belongs to the technical field of environmental microbiology and agricultural science. The method comprises the following steps: diluting livestock liquid dung or livestock feces with water; after hydrolysis and acidification treatment, performing standing and settlement for desilting; mixing a supernatant obtained with a photosynthetic bacterial solution for anaerobic culture; using the mixed solution as a base liquid and stepwise adding lactobacilli, saccharomyces, bacillus thuringienesis and actinomycete into the base liquid in a proportion; and after anaerobic culture and aerobic treatment, finally filling the mixture into a biological fluidized bed for further treatment to obtain the high-efficiency composite microbial agent. The microbial agent is rich in the photosynthetic bacteria, the lactobacilli, the saccharomyces, the actinomycete, the bacillus thuringienesis, azotobacter, nitrobacteria, denitrifying bacteria and other beneficial organisms, and be widely used in agriculture, livestock breeding, fresh water aquaculture, organic sewage treatment, microbial deodorization, and other aspects. The technology is suitable to be used by various livestock farms (raisers) for liquid dung treatment.

The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and / or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

The invention discloses a fermented feed additive, a preparation method and application thereof. The fermented feed additive is prepared by fermenting mixed strains and mixed auxiliary materials consisting of monosodium glutamate protein, bean pulp, bran, cottonseed meal, corn fibers, rice bran and powdered rice hulls, wherein the mixed strains are obtained by mixing activated strains of lactobacillus acidophilus, lactobacillus planetarium, lactobacillus casei, saccharomyces cerevisiae, bacillus subtilis and bacillus licheniformis in a proportion of (1-5): (1-5): (1-5): (1-5): (1-5): (1-5); and the dosage of the fermented feed additive is 5 to 100 percent of the weight of the animal feed when used for the animal feed. According to the fermented feed additive, the bean pulp, the bran, the cottonseed meal and other raw auxiliary materials are sufficiently utilized, so that the food consumption in the animal feeding process is reduced; the fermented feed additive can be used to partiallyor completely replace the animal feed, and antibiotic medicaments can be reduced or prevented from being used; and no medicament is left, so that the feeding cost of farmers is reduced.

The present invention relates to methods for treating and completely curing fungal, yeast, and / or mold infections in human subjects comprising topically administering to a human subject in need thereof an antifungal nanoemulsion composition.

The present invention is directed to novel telomerase nucleic acids and amino acids. In particular, the present invention is directed to nucleic acid and amino acid sequences encoding various telomerase protein subunits and motifs, including the 123 kDa and 43 kDa telomerase protein subunits of Euplotes aediculatus, and related sequences from Schizosaccharomyces, Saccharomyces sequences, and human telomerase. The present invention is also directed to polypeptides comprising these telomerase protein subunits, as well as functional polypeptides and ribonucleoproteins that contain these subunits.

A biological deodouring process for the stink gas features that the stink gas is forced to pass through the biologic filter material prepared from anticorrosion plant fibres, porous materia, or theirmixture through inoculating microns (yeast, bacteria, etc) to it. Its apparatus is composed of blower, gas washing pool and biological filter tower. Its advantages are simple apparatus, high effect, low cost, and no secondary pollution.

The invention relates to a quick-acting multi-microorganism composite water flush fertilizer and a preparation method thereof. The quick-acting multi-microorganism composite water flush fertilizer is characterized by being prepared by mixing bacterial powder which is prepared by fermenting and culturing photosynthetic bacteria, pichiafermentanslodder, lactic acid bacteria, bifidobacterium, bacillus subtilis, bacillus licheniformis, brevibacillus laterosporus, bacillus megaterium, bacillus mucilaginosus, azotobocter chroococcum and actinomycetes. The density of the pichiafermentanslodder ACCC20319T in the quick-acting multi-microorganism composite water flush fertilizer is more than or equal to 2.0*10<8>cfu / g, and the densities of other bacteria are more than or equal to 2.0*10<9>cfu / g. The quick-acting multi-microorganism composite water flush fertilizer can improve the quality and yield of crops and shorten growth period of crops.

The invention discloses a method for brewing a fruit wine. The raw materials of the fruit wine comprise the following components by weight percentage: 71wt%-86wt% of fruit crude juice, 9wt%-21wt% of white sand sugar, 4wt%-6wt% of yeast strain amplification culture solution and 1wt%-2wt% of lactobacillus strain amplification culture solution. The technological process of the method comprises the steps as follows: (1) juicing fruits, filtering and adding sulfur dioxide (SO2); (2) clarifying the fruit juice; (3) carrying out the primary fermentation; (4) carrying out the secondary fermentation; (5) clarifying the crude wine; (6) blending; (7) carrying out the cold treatment; and (8) sterilizing and canning. The method has the advantages that the strain amplification culture solution is subjected to the primary fermentation and the secondary fermentation, therefore, the fermentation speed is high, the fermentation time is short, and the fruit wine has good original taste and good stability; and the bentonite-loaded chitosan is adopted to carry out the clarifying treatment and a diatomite filter is used for filtering, thereby the fruit wine has pure original taste; and the 6-second flash sterilization at the high temperature of 105 DEG C is carried out to prolong the shelf life of the fruit wine.

The invention discloses probiotic whitening recovery mask essence which comprises five natural probiotic active ingredients, namely saccharomyces lysate, leuconostoc / radish root fermented product filtrate, lactobacillus fermentation lysate, bifida ferment product lysate and acidophil / grape fermentation product respectively. The essence is high in skin-friendly property and fast in permeation, and the skin is safely and effectively whitened for a long time. The invention also discloses a method for preparing the probiotic whitening recovery mask essence.

A mixture of probiotics effective to reduce the contamination of enteric bacteria in humans and other monogastric animals. The mixture of probiotics includes one or more acid-producing bacteria strains and one or more yeast strains, and may advantageously be supplemented with a source of nutrients, such as prebiotics including fructo-oligosaccharides. In a preferred embodiment, said one or more bacteria strains contain Enterococcus faecium strain NCIMB #10415, and said one or more yeast strains contain NCYC #47 and CNCM I-1079.

The invention discloses a fermented pepper sauce and preparation method thereof, comprising cleaning and cutting the greenly red pepper; blanching; pulping; adding ginger, garlic, tomato, carrot, carbon source, salt as additive materials; sterilizing; and then inoculating the mold or yeast or compound bacteria of mold and yeast to perform alcohol fermentation; and then sterilizing; inoculating the acetic acid bacteria to perform acetic acid fermentation; finally adding sweeting agent and thickener to be canned. After blanching, the pulping is not necessary and the calcium salt is added to produce the products during process of preparing. The fermented pepper sauce has gentle pungent taste, delicious taste, fermentation flavor. The preparation method thereof can quickly produce the fermented pepper sauce with good and stable quality, fine flavor, safety without antiseptic, suitable for industrialized mass production with excellent market application foreground.

The invention relates to integrated enzyme powder having effects of reducing fat and losing weight. The integrated enzyme powder is characterized by being prepared from powders which are, by weight parts, 62.8-71.8 parts of integrated enzyme raw powder, 8-15 parts of konjac flour, 10 parts of pumpkin powder, 1 part of papaya powder, 3 parts of pineapple powder, 1 part of mango powder, 1 part of sweet orange powder, 1 part of snow pear powder, 3-5 parts of dietary fiber powder and 0.2 part of compound lactobacillus powder. 43 fruits, 20 vegetables, 4 cereal nuts and 11 medicinal and edible herbaceous plants and natto are selected in total to serve as fermentation raw materials; through yeast and lactobacillus fementation, generated enzymes are total nutrients needed by humans, and the enzyme powder contains activity probiotics which are resistant to acid and heat; and through testing, the probiotics in artificial gastric juice still have ultrahigh vigor; at normal temperature (37 DEG C), the probiotics still maintain activity, can regulate intestinal flora of the human body, promote digestion and avoid constipation. The mixed integrated enzyme powder not only can achieve the goal of losing weight, but also can meet the basic nutrition requirements of the human body, and it is a healthy and effective method of reducing fat and losing weight to use the enzyme powder.

Novel genes encoding P. pastoris ARG1, ARG2, ARG3, HIS1, HIS2, HIS5 and HIS6 are disclosed. A method for inactivating alternately at least two biosynthetic pathways in a methylotrophic yeast is provided. A method for producing and selecting yeast strains characterized as being capable of genetic integration of heterologous sequences into the host genome using the genes involved in the biosynthetic pathways is also disclosed.

The present invention relates to methods for treating and completely curing fungal, yeast and / or mold infections in human subjects comprising topically administering to a human subject in need thereof an antifungal nanoemulsion composition.