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Compound microbial culture starter and application thereof

A technology of compound microorganisms and starters, applied in the direction of microorganisms, microorganisms, methods based on microorganisms, etc., can solve the problem of affecting the application effect and promotion and application process of fermentation bed cultivation, restricting the promotion and application of fermentation bed cultivation technology, and increasing the application of high input prices Cost and other issues, to achieve stable application effects, improve animal resistance, and highly targeted effects

Inactive Publication Date: 2010-06-30
河北瑞塞可环保科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Micro-ecological fermentation bed farming technology is widely recognized by the government and farmers due to its advantages in energy saving, emission reduction, environmental protection, safety, and welfare. Limits the popularization and application of fermentation bed culture technology
At present, there are many commercial products of special starter for ecological fermentation bed culture technology, and the price is high. The cost of starter per square meter of litter is 30-60 yuan, and the product quality is uneven, and the application effect is often inconsistent. The stable phenomenon seriously affects the application effect and popularization and application process of fermentation bed culture

Method used

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  • Compound microbial culture starter and application thereof
  • Compound microbial culture starter and application thereof
  • Compound microbial culture starter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Cultivation of strains

[0041] 1. Yeast culture method

[0042] Preparation of culture solution: yeast extract 0.5%, peptone 0.5%, glucose 0.5%, in a production tank, heated to 90°C, and kept at 90°C for 2 hours. Then cool to about 33°C. Access to 0.01% Saccharomyces cerevisiae original bacteria (Saccharomyces cerevisiae CICC 31011 Sichuan Food Fermentation Industry Research and Design Institute).

[0043] Cultivation conditions: 33°C to 34°C with stirring for 12 hours.

[0044] The produced yeast liquid is put into 4℃ refrigeration for later use.

[0045] 2. Bacillus subtilis cultivation method

[0046] 2.1 Cultivation of strains

[0047] 2.1.1 Preparation of culture medium

[0048] Plate solid medium: beef extract 0.5%, yeast extract 0.5%, glucose 0.5%, peptone 1%, NaCl 0.5%, agar 2%, diluted with distilled water or non-ionized water, pH 7.2.

[0049] Liquid culture medium: Soy Peptone 2%, Sucrose 2%, Disodium Hydrogen Phosphate 0.2%, Sodium Dihydrogen Phosphate 0.1%, Mangan...

Embodiment 2

[0074] Example 2 Assemble a composite microbial starter

[0075] Step 1. Mix the bacterial liquid prepared in Example 1 in the following volume ratio:

[0076] Bacillus subtilis: Bacillus natto: yeast: lactic acid bacteria=3:3:3:1 ratio is mixed, stabilizer is added according to a volume-to-mass ratio of 1 / 10,000, sealed, and stored under dark conditions.

[0077] The stabilizer is prepared by mixing sodium dihydrogen phosphate: disodium hydrogen phosphate at a mass ratio of 2:1.

[0078] Step 2. Product testing

[0079] The number of live yeast ≥10 8

[0080] The number of viable Bacillus subtilis ≥10 7

[0081] The number of viable bacteria of Bacillus natto≥10 7

[0082] Number of live lactic acid bacteria≥10 7

Embodiment 3

[0083] Example 3. Application of composite microbial starter

[0084] Step 1 Multiplication

[0085] 1. Take a container of more than 10 kg, such as a water tank, a plastic bucket, or a stainless steel bucket, and it must be cleaned without any greasy or chemical residues.

[0086] 2. Pour 1 kg of brown sugar into 10 kg of water, stir until it is fully dissolved and boil, then naturally cool to about 35°C, add 1 kg of the composite microbial starter prepared in Example 2, and then culture it naturally.

[0087] 3. During the cultivation process, keep the ambient temperature at 30-35°C, stir once every 4-8 hours, and it will become probiotics after 48 hours.

[0088] 4. After the cultured bacteria liquid is stirred, a small mineral water bottle can be used to scoop out a bottle of the bacteria liquid for observation. If the bacteria liquid has a better propagation effect, a thin layer of white sediment will appear on the bottom of the bottle after a few hours.

[0089] (2) Matters needing...

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Abstract

The invention discloses a compound microbial culture starter and application thereof, which belong to an application of a beneficial microbial preparation at low cost. The active microorganism of the compound microbial culture starter comprises saccharmyces cerevisiae, bacillus subtilis, bacillus natto and lactococcus lactis. The compound microbial culture starter can be used for livestock and poultry raising beds, feeds addictive and other aspects and is characterized by reducing the cost input of users on the culture starter and having stable effect.

Description

Technical field [0001] The invention relates to the application of a beneficial microbial starter in the field of livestock and poultry breeding, in particular to a composite microbial starter and its application. technical background [0002] Microbial technology is widely used in the field of healthy livestock and poultry breeding. The principle of microecological engineering is used to screen out beneficial microbial strains suitable for different livestock and poultry needs. After compounding and other processes, microecological preparations are made and added to drinking water or feed to play a It can increase production, deodorize and improve meat quality. At present, composite microbial preparations are widely used in pigs, cattle, rabbits, dogs, chickens, ducks, geese, pigeons, fish, turtles, shrimps and almost all livestock and poultry breeding. [0003] The role of compound microorganisms in livestock and poultry breeding can be classified as follows: (1) Increase livest...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00C12N1/20C12N1/18A23K1/16A01K1/015A61L9/01C12R1/125C12R1/07C12R1/01C12R1/865A61L101/52
Inventor 王海宏潘裕华刘华贵耿爱莲张剑初芹肖银花
Owner 河北瑞塞可环保科技有限责任公司
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