7 results about "Bacillus natto" patented technology

The application provides a bacillus natto and application thereof in preparation of functional feed for Chinese mitten crab breeding, and belongs to the technical field of aquaculture. Bacillus natto HQ-1 has been preserved in China Center for Type Culture Collection on August 11, 2025, and the preservation number is CCTCC No. M 20251811. Research shows that the bacillus natto has a water product pathogenic bacteria inhibition effect, a toxin degradation capacity and a water body environment purification function which are significantly improved compared with the same strain. The functional feed prepared by fermentation of the strain contains bioactive ingredients such as probiotic live bacteria, free amino acids, antibacterial lipopeptides and polyglutamic acid, can improve the nutritional form of the feed, improve the body weight, disease resistance and feeding rate of the Chinese mitten crab, and help to reduce the content of harmful substances in the aquaculture water body and improve the water body environmental quality. The application provides a new technical approach for green, antibiotic-free and sustainable breeding of the Chinese mitten crab.

This invention belongs to the field of turfgrass and forage grass maintenance technology, specifically relating to a microbial selenium protein preparation for alleviating drought stress in perennial ryegrass, its preparation method, application method, and application. The microbial selenium protein preparation comprises the following raw materials in specific weight proportions: peptone, beef extract, sucrose, salt, selenium-enriched mushroom powder, organosilicon, calcium amino acids, potassium humate, betaine, salicylic acid, and Bacillus natto. This microbial selenium protein preparation aims to solve the limitations of traditional drought-resistant methods and the poor effectiveness of single-component preparations. When using this microbial selenium protein preparation, it is first diluted and then applied as a root drench to ryegrass seedlings multiple times. The selenium protein preparation of this invention, through the synergistic effect of multiple components, can significantly improve the drought resistance of ryegrass. Furthermore, the preparation process of this protein preparation is simple, environmentally friendly, and efficient, suitable for ryegrass propagation and maintenance, and has broad application prospects.

The application discloses a chicken feed additive rich in DHA and having liver protection function, and a preparation method and application thereof. The chicken feed additive contains the following components in parts by weight: 40-60 parts of double-source DHA reinforcing component, 20-30 parts of plant synergistic liver protection component, and 5-10 parts of microecological and metabolic regulator. The double-source DHA reinforcing component is compounded from compound DHA microcapsules and squid egg powder. The plant synergistic liver protection component is a compound product formed by synergistic fermentation of sea buckthorn seed meal, spilanthes acmella and pineapple residue by bacillus natto. The microecological and metabolic regulator contains bacillus natto powder, vitamin E powder and tea polyphenol powder. The DHA content of the chicken eggs produced by the method can reach 250 mg / 100 g or more, and the proportion of phospholipid type DHA in the total DHA is greater than or equal to 35%. Meanwhile, the liver function of the laying hens is normal, the liver index and fat deposition rate are maintained in the normal physiological range, and the body health of the laying hens is effectively ensured.

This invention provides a composition with hepatoprotective and intestinal-protective effects, its preparation method, and its application, belonging to the field of aquaculture technology. The composition with hepatoprotective and intestinal-protective effects provided by this invention is made from the following raw materials: Gynostemma pentaphyllum, Hippophae rhamnoides, Euryale ferox, pumpkin seeds, Polygonatum odoratum, Scutellaria baicalensis, Houttuynia cordata, and a compound microbial agent, wherein the compound microbial agent is composed of Candida utilis, Bacillus natto, and Saccharomyces cerevisiae. The composition provided by this invention is entirely non-toxic and contains no prohibited ingredients. Its medicinal properties are balanced in terms of cold and hot properties, avoiding the damage to the spleen and stomach of aquatic animals caused by long-term feeding of traditional cold-natured medicinal materials. It can be added to feed regularly throughout the entire aquaculture cycle, meeting the requirements of green antibiotic-free aquaculture, and there are no drug residues or withdrawal period restrictions. Furthermore, it can achieve bidirectional synergistic regulation of the liver and intestines, with comprehensive target action. In addition, the composition provided by this invention can not only enhance the stress resistance of aquatic animals and reduce the incidence of aquaculture diseases, but also improve digestive and absorptive functions, thereby increasing the efficiency of aquaculture production.

This invention relates to the field of tofu processing technology, and discloses a multi-bean-based odorless, high-activity freeze-dried natto tofu and its preparation method. The method includes the following steps: 1) Raw material processing: selecting one or more types of beans, screening to remove impurities, soaking, dehydrating, and peeling; 2) Slurry preparation: grinding and filtering the peeled beans, then adding auxiliary materials and homogenizing to obtain soy milk; 3) Segmented temperature-controlled fermentation: cooling the soy milk to 37°C, inoculating with Bacillus natto bacterial solution, stirring evenly, and then fermenting in segments under controlled temperature; 4) Shaping and cutting: injecting the fermented soy milk into molds and cutting to form tofu blocks; 5) Quick-freezing: quick-freezing the tofu blocks at -45°C ± 2°C; 6) Gradient vacuum freeze-drying: subjecting the quick-frozen tofu blocks to gradient vacuum freeze-drying; 7) Packaging and inspection: packaging and inspecting the freeze-dried tofu blocks. The natto tofu produced by this invention eliminates the beany and ammonia odors, maintains both activity and taste, and has long shelf life.

The application provides high-SDF wheat bran powder and a preparation method and application thereof. The steps are as follows: S1. high-quality wheat bran is taken, washed, dried, ultra-finely pulverized and sieved to obtain wheat bran powder; S2. water is added to the wheat bran powder, cellulase is added, ultrasonic treatment is carried out, and enzyme hydrolysis is carried out, 80 DEG C short-time enzyme inactivation is carried out, and the fermentation substrate is obtained after cooling; S3. the water content of the substrate is adjusted, and constant-temperature fermentation is carried out in stages at 37 DEG C: stage one is aerobic fermentation by inoculating Bacillus natto; stage two is anaerobic fermentation by adding sterile water to adjust the water content, introducing nitrogen, inoculating Lactobacillus plantarum and adding food-grade ferulic acid esterase; S4. the fermentation product is subjected to vacuum freeze-drying, ultra-fine pulverization and sieving, and the high-SDF wheat bran powder is obtained. The application breaks through the bottleneck of the disconnection between pretreatment and fermentation, low enzyme utilization rate and non-targeted product conversion in the existing process by using the mild enzyme-preserving pretreatment-bacteria-enzyme synergistic stage-by-stage fermentation technology, realizes efficient value-added modification of wheat bran resources, and expands the application scene of the wheat bran in low GI functional food.

The present application relates to a kind of compound that can degrade multiple corn mycotoxins simultaneously, belong to the technical field of food safety.The compound is composed of mixed strains and biological enzymes;With the total mass of mixed strains being 100%, the composition and mass fraction in mixed strains are as follows: Bacillus natto 25%-45%, Bacillus amyloliquefaciens 5%-25%, Saccharomyces cerevisiae 10%-30%, Lactobacillus plantarum 10%-30%;Biological enzyme is solid cellulase and xylanase;The compound uses the biological technology of bacteria and enzyme synergistic use, eliminates the health hazards brought by chemical detoxification, and does not produce toxic by-products in the degradation process;The compound is added in proportion in the corn contaminated by fungi, and has significant degradation effect on aflatoxin B1, zearalenone, vomitoxin, fumonisin B1 and ochratoxin A.