Microbial fertilizer manufacturing method, prepared microbial fertilizer and compound microorganism preparation

A technology of microbial bacterial fertilizer and compound microorganisms, applied in the field of biochemistry, can solve the problems of soil water retention, fertilizer retention capacity and permeability reduction, soil compaction, etc., so as to shorten the cultivation time, reduce the production cost and improve the yield.

Active Publication Date: 2015-12-02
嘉兴市中正置业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The destruction of soil aggregate structure leads to the reduction of soil water ret

Method used

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  • Microbial fertilizer manufacturing method, prepared microbial fertilizer and compound microorganism preparation
  • Microbial fertilizer manufacturing method, prepared microbial fertilizer and compound microorganism preparation
  • Microbial fertilizer manufacturing method, prepared microbial fertilizer and compound microorganism preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Raw materials

[0053] Antibiotic residue: Penicillin G residue from a pharmaceutical factory, after testing, has a water content of about 75.9%, and the content of penicillin G in the residue is 2000ppm;

[0054] Wastes rich in plant fibers: residues after extraction of Houttuynia cordata and licorice;

[0055] Composite microbial strains: Cellulomonas flavogenes, Bacillus natto, Saccharomyces cerevisiae, Lactobacillus plantarum;

[0056] Microbial culture medium: potato residue, distilled water, urea, KH 2 PO 4 、K 2 HPO 4 .

[0057] 2. Fermentation of microbial strains

[0058] The preparation of potato residue medium, potato residue 100g, distilled water 200g, urea 2g, KH 2 PO 4 0.2g, K 2 HPO 4 0.5g; Microbial strain inoculation, under sterile conditions, inoculate Cellulomonas flavinogenes, Bacillus natto, Saccharomyces cerevisiae, and Lactobacillus plantarum into potato dregs medium respectively, at 100rpm, the temperature is 25 ℃, pH is 6.8 under the...

Embodiment 2

[0067] 1. Raw materials

[0068] Antibiotic residue: Cephalosporin C residue from a pharmaceutical factory has been tested and found to have a water content of about 83.9%, and the cephalosporin content in the residue is 1800ppm;

[0069] Wastes rich in plant fibers: corn stover;

[0070] Composite microbial strains: Streptomyces, Bacillus natto, Saccharomyces cerevisiae, Lactobacillus plantarum;

[0071] Microbial culture medium: potato residue, distilled water, urea, KH 2 PO 4 、K 2 HPO 4 .

[0072] 2. Fermentation of microbial strains

[0073] The preparation of potato residue medium, potato residue 100g, distilled water 200g, urea 2g, KH 2 PO 4 0.2g, K 2 HPO 4 0.5g; microbial strain inoculation, under sterile conditions, inoculate Streptomyces, Bacillus natto, Saccharomyces cerevisiae, and Lactobacillus plantarum respectively in potato dregs medium, at 100rpm, the temperature is 25°C, and the pH is Under the condition of 6.8, culture on a shaking table for 2 days...

Embodiment 3

[0081] 1. Raw materials

[0082] Antibiotic residue: Tetracycline residue from a pharmaceutical factory, after testing, has a water content of about 80.4%, and the tetracycline content in the residue is 1600ppm;

[0083] Wastes rich in vegetable fibers: distiller's grains;

[0084] Composite microbial strains: Nocardia cellulose, Bacillus natto, Saccharomyces cerevisiae, Lactobacillus plantarum;

[0085] Microbial culture medium: potato residue, distilled water, urea, KH 2 PO 4 、K 2 HPO 4 .

[0086] 2. Fermentation of microbial strains

[0087] The preparation of potato residue medium, potato residue 100g, distilled water 200g, urea 2g, KH 2 PO 4 0.2g, K 2 HPO 40.5g; Microbial strain inoculation, under sterile conditions, inoculate Nocardia cellulose, Bacillus natto, Saccharomyces cerevisiae, and Lactobacillus plantarum into potato dregs medium respectively, at 100rpm, the temperature is 25 ℃, pH is 6.8 under the conditions, shaker culture 2d; obtain the enrichment ...

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Abstract

The invention provides a microbial fertilizer manufacturing method. Antibiotic fungus residue serves as a base material, and compound microorganisms are adopted to perform solid fermentation on materials, wherein the materials comprise waste rich in plant fiber and the antibiotic fungus residue. The compound microorganisms comprise cellulose decomposition bacteria and one or more of bacillus natto, saccharomyces cereviseae and lactobacillus plantarum. According to the microbial fertilizer manufacturing method, the problem that it is difficult to treat the fermented antibiotic fungus residue in a pharmaceutical factory at present can be solved. Furthermore, beneficial ingredients in the antibiotic fungus residue are can be utilized and converted into a microbial fertilizer, so as to solve the problem that at present, the soil hardening occurs and the fertility declines.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for preparing microbial fertilizer, which uses antibiotic residue as a base material. Further, it also relates to the microbial bacterial fertilizer prepared by the microbial bacterial fertilizer, which can be used for soil remediation; and a compound microbial preparation. Background technique [0002] my country is the world's largest producer of antibiotic raw materials. For the bacterial residue produced by fermentation method, most pharmaceutical factories use incineration, landfill and other methods to dispose of it. However, these methods will cause varying degrees of pollution to the atmosphere, soil, and groundwater. The antibiotic residue in the bacterial residue will cause animals and plants to Antibiotic resistance has an indirect impact on human health. Fermented antibiotic residues are rich in nutrients and various trace elements. The content of cr...

Claims

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Application Information

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IPC IPC(8): C05F11/08C12N1/20C12N1/18C12N1/14C12R1/465C12R1/365C12R1/885C12R1/145C12R1/07C12R1/865C12R1/25
Inventor 段得振吴力克边聪聪何菁
Owner 嘉兴市中正置业有限公司
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