169 results about "Nocardia" patented technology

The invention discloses a multifunctional microorganism soil repairing agent as well as preparation and application thereof and belongs to the fields of biological techniques and environment protection. The multifunctional microorganism soil repairing agent is made of the following raw materials in parts by weight through uniform mixing: 30-60 parts of nano chitosan, 10-20 parts of humic acid, 10-20 parts of nano charcoal and 20-30 parts of a composite microbial agent, wherein the composite microbial agent is prepared from fresh benzene bacillus, B.sonorensis, lysinibacillus sphaericus, bacillus licheniformis, bacillus circulans, bBacillus thuringiensis, achromobacter denitrificans, saccharothrix australiensis, Rhodococcus zopfii, pseudonocardia sulfidoxydans and pseudonocardia dioxanivorans through purification, optimization, fermentation and drying dehydration. The multifunctional microorganism soil repairing agent disclosed by the invention is capable of replacing conventional physical and chemical repairing techniques, is capable of degrading complex pollutants, is wide in repairing range, is capable of preventing soil degradation and inhibiting diseases and insects in environments, and has the advantages of being good in repairing treatment effect, low in cost and economic and environment-friendly.

The invention relates to an application of nocardia rubra-cell wall skeleton in the preparation of the medicines used for treating skin injury and furuncles, including skin ulcer and ulceration.

The present invention relates to thermostable proteases having an amino acid sequence which homologous to the amino acid sequence of proteases derived from Nocardiopsis, and the production thereof by wild-type and recombinant host cells including transgenic plants and non-human transgenic animals. The proteases are effective in animal feed, in particular fish feed, and detergents. The proteases are capable of degrading the soybean Bowman-Birk inhibitor, and other antinutritional factors such as soybean agglutinin and the Kunitz trypsin inhibitor, as well as the isolated soy storage proteins glycinin and beta-conglycinin. Characteristic structural features of relevance for the thermostability of these proteases of peptidase family S2A or S1E are disclosed.

The invention provides bacillus velezensis and application of the bacillus velezensis as an aquatic pathogenic bacteria inhibitor. The bacillus velezensis is separated from the intestinal canal of healthy tilapia, and the preservation number of the bacterial strain is GDMCC No. 60344. The bacillus velezensis has a broad-spectrum antagonistic aquatic common pathogenic bacteria function, and has theadvantages that the growth of the pathogenic bacteria, such as streptococcus agalactiae, streptococcus iniae,nocardia seriolea, aeromonas hydrophila, aeromonas schubertii and edwardsiella tarda, canbe effectively inhibited, the use is safe, no residue is generated, reducing the usage amount and the residual amount of chemical drugs in aquaculture is facilitated, the production cost is reduced, and the practicality is strong.

The invention relates to a novel microbial organic fertilizer with anti-actinomyces active compound bacteria and a preparation method thereof. The novel microbial organic fertilizer comprises a microbial inoculum and an organic fertilizer, wherein the microbial inoculum comprises colonies of Bacillus siliceous, Nocardia actinomyces, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus laterosporus, azotobacter, Beauveria bassiana, Metarrhizium anisopliae, Trichoderma and Saccharomyces, and peptide proteins and rare-earth elements having unique effects on inhibiting wireworms and enhancing plant root system stress tolerancen; and the organic fertilizer comprises animal manure and plant fibers. The novel microbial organic fertilizer is prepared by fermenting the animal manure and plant fibers by using the microbial inoculum. The microbial organic fertilizer can quickly and effectively kill wireworm ova in soil, has strong effects on inhibiting and killing larvae and imagos, can continuously improve the growth environment of the plant root by using the growth of the microbial colonies and the stress tolerance of the peptide proteins, enables the plant to have strong roots, thick stems and green leaves, postpones the aging of the plant, and promotes prematurity and yield increase.

The invention discloses a gene chip of aquatic product cultivation pathogenic bacterium, comprising a solid phase carrier which is modified chemically, a detection probe and a quality control probe are distributed on the solid phase carrier in a dot matrix way; the detection probe comprises specificity 16S rDNA sequences and/or gyrB gene sequences of vibrio, comma bacillus, vibrio harveyi, vibrio alginolyicus, vibrio anguillarum, vibrio parahemolyticus, nocardia, nacardia seriolea, aeromonas, hydrophilic aeromonas, streptococcus and dolphin streptococcus, which are to be detected, the quality control probe includes PCR positive, chip fixed positive control, chip hybridizing negative control, chip hybridizing positive control and chip hybridizing blank control; the gene chip has the advantages of small volume and high flux, can detect known and unknown germs of the vibrio, the nocardia, the aeromonas and the streptococcus, and can detect specific germs with multiple kinds, and the simpleness and rapidness and specificity of the germs can be detected, and automatic detection can be carried out after detection software is additionally arranged.

The invention relates to a method for treating or preventing (including immunising against) post-weaning multisystemic wasting syndrome (PMWS) and/or porcine dermatitis and nephropathy syndrome (PDNS) in a subject comprising administering an effective amount of a pharmaceutical composition or immune modulator composition comprising a whole cell of a bacterium from one or more of the genera Rhodococcus, Gordonia, Nocardia, Dietzia, Tsukamurella and Nocardioides, to said subject. In addition the method relates to the use of an immune modulator composition or a pharmaceutical composition comprising a whole cell of a bacterium from the genera Rhodococcus, Gordonia, Nocardia, Dietzia, Tsukamurella and Nocardioides, in the manufacture of a medicament for the treatment or prevention of post-weaning multisystemic wasting syndrome (PMWS) and/or porcine dermatitis and nephropathy syndrome (PDNS).

The invention relates to a compound microorganism bacterium agent used for sludge reduction, a preparation method and application of the compound microorganism bacterium agent. The compound microorganism bacterium agent is a liquid bacterium agent prepared by fermenting denitrified pseudomonas, nocardia coralline, candida utilis, rhodobacter sphaeroides and aspergillus sojae. According to the invention, the metabiosis action between microbe consortia is utilized to form a biodegradation system in which the activities of various required enzymes are all high, so dead or decrepit thalli in activated sludge can be effectively damaged and decomposed, and meanwhile, organic matters can be decomposed; and the generation quantity of excess sludge is reduced through reducing the quantity of the sludge in the process without influencing the quality of yielding water. On the premise of not greatly changing the traditional sewage treatment process, the sludge reduction ratio can reach 30%-70% in the sewage treatment process by adopting the compound microorganism bacterium agent, and the comprehensive running cost of the sludge treatment can be reduced, so that the compound microorganism bacterium agent is of great significance on the aspects of economic, environmental and social benefits.

The invention discloses a formula of a water body remediation microbial inoculant and a preparation method of the remediation microbial inoculant, and particularly relates to the field of sewage treatment. The water body remediation microbial inoculant comprises a microbial agent and a carrier, wherein the microbial agent comprises nitrobacteria, denitrifying bacteria, fibrotic nocardia, lactobacillus plantarum, candida utilis, bacillus subtilis and bacillus mucilaginosus, and the carrier comprises wheat bran, zein and zeolite powder. Though adding the complex inoculantwhich is extremely easy in survival and reproduction into the water body remediation microbial inoculant, a beneficial dominant community is formed, and the ecological structure of microorganisms in a water body is regulated and controlled; the microbial inoculant can degrade and transform the harmful substances in polluted water, promote the growth of indigenous flora, and play a role in purifying water quality, more importantly, various microbial flora contained in the microbial inoculant can promote the growth of aquatic plants, prevent and control aquatic animal diseases, and inhibit excessive reproduction of algae, and finally the effect of stabilizing and restoring the ecological environment of the water body is achieved.

The present invention relates to a biological preparation, in particular, it is a red nocardia actinomycetes cell wall skeleton preparation. Firstly, culturing red nocardia actinomycetes CGMCC No.0712, making enrichment, culture, collecting thallus, breaking cell, enzyme purification, removing fat, blending, filling and freeze-drying so as to obtain the invented product containing red nocardia actinomycetes cell wall skeleton and dextran 40 according to a certain mixing proportion. The tests and clinical results show that its therapeutic effect is obvious for curing indications.

This invention relates to the isolation of thermophilic phytase-producing microorganisms, method for producing phytase using such microorganisms, phytase obtained therefrom, and usage of the phytase to hydrolyze phytic acid or phytate. In particular, this invention relates to phytase-producing microorganisms, which belong to Streptomyces sp., Pseudonocardia sp. or Microbispora sp., and which produce phytase available for recovery in an efficient and practical manner.

A method of treating cancers by administering to a patient an anti-tumor effective amount ot at least one of a thermostable macromolecular antigen complex or a fragment of such a complex which is interspecific for microorganisms of the Mycobacteria, Nocardia and Corynebacteria group and which exhibits after immunoelectrophoresis an immunoelectrophoretic precipitation pattern corresponding to that of the antigen complex 60 of Mycobacteria bovis Calmette Guerin Bacillus strain. Preferably, the patient is also treated with an additional therapeutic agent which is specific against the patient's cancer such as a tumoral antigen or a non-proliferative tumor cell.

The invention discloses a microbiological bacterium and method for making same, wherein the microbiological bacterium comprises degreased saccharomycete, Trichoderma viride, high temperature bacillus, S. melanoferus, Nocardia and solid medium. The microbiological bacterium can be utilized as dejection treatment agent. Its preparation process comprises the three stage pure culture of these bacterial strains, and proportional mixing of the culture.

The invention discloses a microbiological preparation for efficiently treating municipal lake water pollution. The microbiological preparation is prepared from nitrosospira, rhodospirillum, bacillus subtilis natto, kitasatospora phosalacinea and lactobcillus in a volume ratio of 0-10 to 5-15 to 5-15 to 5-15 to 5-15. The microorganism selected and cultured by the invention has the capability of being rapidly multiplied and developed into a dominant microflora under the municipal lake water environment; and each strain can independently and cooperatively decompose eutrophic ingredients in the municipal lake water and can make certain eutrophic ingredients which are difficult to decompose in a normal state easy to decompose. The microbiological preparation has the advantages of purifying the water quality well, improving the substrate, optimizing the biological environment, saving the biological environment from damage, having obvious effect on water raising, water retention, water color stabilization, treatment of turbid water, water concentrate, red water, black water, bottom odourization and algae aging and regulation and stabilization of pH value of the water body and making the lake water clear and odorless rapidly, along with simple using method, low cost, obvious effect, no secondary pollution.

The invention discloses a detection primer set, detection kit and detection method for Vibrio parahaemolyticus, Photobacterium damsela and Nocardia seriolea. The primer set comprises a primer pair V.pa, a primer pair N.se and a primer pair P.da, and the nucleotide sequences of the primer pairs are disclosed as SEQ ID NO.1-6. The detection kit comprises the detection primer set. The detection method uses the detection kit and comprises the following steps: carrying out pretreatment on a sample, extracting genome DNA (deoxyribonucleic acid) as a sample set DNA, and detecting the sample by a PCR (polymerase chain reaction) process. The triplex PCR detection method established by the invention can be used for tracing detection on Vibrio parahaemolyticus, Photobacterium damsela and Nocardia seriolea in various stages, avoids transmission and prevalence of pathogens, and enhances the scientific management efficiency, thereby having very high practical value.

The invention discloses a sludge leavening agent. The sludge leavening agent is prepared from the following raw materials in parts by weight: 15-25 parts of bacillus subtilis, 10-20 parts of bacillus stearothermophilus, 8-15 parts of lactic acid bacteria, 5-10 parts of silicate bacteria, 4-10 parts of nocardia, 9-16 parts of a zotobacter chroococcum, 1-6 parts of saccharomyces cerevisiae, 1-5 parts of flavobacterium oryzae, 2-3 parts of photosynthetic bacteria, 1-3 parts of streptomyces microflavus, 6-13 parts of cellulase, 5-12 parts of amylase and 5-6 parts of xylanase. The sludge leavening agent provided by the invention is environmentally friendly, pollution-free, low in operation cost, small in floor space, and capable of effectively killing pathogenic bacteria, the parasites and the like in the sludge, degrading toxic and harmful substances, accelerating sludge deodorization and dehydration and decomposing nutrition ingredients which are difficult to absorb by crops.

The invention discloses a marine actinomycete strain with meloidogyne insecticidal activity and a preparation method and application of the marine actinomycete strain. The actinomycete strain is Nocardiopsis HA12420 preserved with the preservation number CGMCC No.11063 in China General Microbiological Culture Collection Center on July 9, 2015. The preparation method of an actinomycete fermentation broth includes the steps of seed culture, ferment culture and fermentation broth purification. The actinomycete fermentation broth has distinct insecticidal activity for meloidogyne incognita, meloidogyne javanica and meloidogyne arenaria and is applicable to prevention and control of meloidogyne of vegetables, flowers, melons, crops of orchards and the like, thereby having promising development prospect in agricultural production.