9 results about "Nocardia" patented technology

The application discloses a strain of completely degrading deoxynivalenol (DON), the N25 Nocardioides, the preservation number of the strain being CCTCC M 20252560. Average nucleotide identity (ANI) analysis shows that the strain is a new species in the Nocardioides genus, the strain can utilize DON toxin as a sole carbon source, and can completely degrade the DON toxin to form CO2 and H2O, completely eliminating the toxic effect, and can also degrade a plurality of fusarium toxins including A-type trichothecene toxin neosolensaric acid (NEO) and B-type trichothecene toxin 3-acetyl-DON (3-Ac-DON), nivalenol (NIV) and fusarenon-X (FUS-X). Therefore, the strain has a wide application prospect in the field of fusarium toxin detoxification.

The invention discloses a preparation method and application of an organic waste fermentation inoculant, and belongs to the technical field of microorganisms. The organic waste fermentation inoculant is prepared from the following raw materials in parts by weight: 1 to 3 parts of bacillus subtilis, 2 to 4 parts of bacillus amyloliquefaciens, 2 to 3 parts of pseudomonas fluorescens, 4 to 6 parts of streptococcus thermophilus, 5 to 8 parts of lactobacillus delbrueckii subsp. Bulgaricus, 4 to 6 parts of nocardia albicans, 30 to 40 parts of porous activated carbon, 2 to 3 parts of beet extract, 4 to 6 parts of mannitol and 10 to 15 parts of straw waste powder. The organic waste fermentation inoculant can accelerate the degradation speed of organic matters in the composting process and shorten the fermentation time of the organic wastes, and has remarkable advantages in practical application.

The invention discloses a construction method of a fish nocardia disease pathogenic mechanism model, and belongs to the field of biotechnology and disease models. The invention aims to solve the technical problem that a deep causal mechanism is difficult to reveal due to the fact that microscopic dynamic interaction of pathogens and hosts cannot be observed in real time and in situ when an existing animal model and an in-vitro cell model are used for researching the disease. The method comprises the following steps: constructing a fish immune organ in an intelligent micro-fluidic chip integrated with a micro-actuator as a high-bionic-degree physical experiment platform; the living cell imaging system is used for collecting four-dimensional image data of the organoids in the nocardia infection process in real time. According to the method, through normal form transformation from passive observation to active intervention, the microscopic process of the disease can be reproduced in a high-fidelity manner and is regulated and controlled prospectively, so that a causal chain between key pathological events is deeply revealed, and a powerful intelligent research platform is provided for developing a new disease prevention and treatment strategy.

The application discloses a primer probe combination, a kit and application for detecting Nocardia, a mycobacterium tuberculosis complex and mycobacterium, and belongs to the technical field of genetic engineering. The technical problem to be solved is that there is a lack of a nucleic acid detection tool for Nocardia, the mycobacterium tuberculosis complex and mycobacterium in the prior art, which is high in sensitivity, high in specificity and simple in operation. The technical solution is characterized in that the primer probe combination comprises a primer probe for detecting Nocardia, a primer probe for detecting the mycobacterium tuberculosis complex, a primer probe for detecting mycobacterium and a primer probe for detecting an internal standard control. The primer probe combination can simultaneously screen Nocardia, the mycobacterium tuberculosis complex and mycobacterium, quickly and simply confirms pathogenic species, saves time, manpower and reagent cost, and has the advantages of high specificity, a low minimum detection limit and prevention of false negative or false positive results.

The objective is to provide a wastewater purification solution for seafood processing plants that purifies wastewater, prevents sludge buildup, and prevents foul odors from being generated from the wastewater. [Solution] The solution is a mixture containing a culture medium obtained by culturing an aqueous solution of molasses with 10 species of bacteria from the genera Aspergillus, Bacillus, Lactopranthibacillus, Enterococcus, Nocardia, Pseudomonas, Ralstonia, Rhizopus, Rhodococcus, and Rhodosprillum, as well as iron fulvic acid, molasses, seaweed powder, and water.

The application provides a biomarker for identifying and detecting common pathogenic Nocardia, the biomarker is F6W96_34950, the biomarker is a nucleic acid sequence or an amino acid sequence, the nucleic acid sequence of the biomarker F6W96_34950 is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2. Through identification of the biomarkers, the application can accurately identify and detect Nocardia, and the process is simpler, faster, more accurate and lower in cost, so that at least partial differential diagnosis of diseases caused by Nocardia is realized, and the cure rate and prognosis are improved.

The application provides a set of biomarkers for identifying and detecting Nocardia, wherein the biomarkers are one or more of NCTC10797_02287, NCTC10797_01760, NCTC10797_05842, NOCYR_2299 and C5B73_13220, the biomarkers are nucleic acid sequences or amino acid sequences, the nucleic acid sequences of the biomarkers NCTC10797_02287, NCTC10797_01760, NCTC10797_05842, NOCYR_2299 and C5B73_13220 are shown in SEQ ID NO. 1-5, and the amino acid sequences are shown in SEQ ID NO. 6-10. By identifying the molecular markers, the Gelsenkirchen Nocardia can be accurately identified and detected, and the process is simpler, faster, more accurate and lower in cost, so that the differential diagnosis of diseases caused by the Gelsenkirchen Nocardia is at least partially achieved, and the cure rate and prognosis are improved.

The invention discloses a combined flora SynCom4 capable of degrading fatty acid and application of the combined flora SynCom4. The combined flora consists of nocardia globulus DSM 44596, citrobacter fabricius CDC 2991-81, serratia marcescens NBRC 102204 and an eleostearic acid degrading strain 02 according to an equal proportion. When the combined flora is added into tung tree fruit compost, the content of fatty acid can be reduced, the content of total nitrogen, total phosphorus and total potassium can be increased, and the compost nutrient structure and fertilizer efficiency can be optimized; meanwhile, the fermented and decomposed tung tree fruit compost is applied to the tea garden soil, the soil microbial community structure can be improved, the soil micro-ecological stability can be improved, the soil fertility and sustainable utilization can be enhanced, collaborative development of tea garden ecological protection and high-quality planting is achieved, and the tea garden ecological protection and high-quality planting are promoted. An efficient and environment-friendly technical scheme is provided for resource utilization of tung tree fruit waste and ecological planting of a tea garden, and the method has important practical application value and popularization prospects.

The invention discloses a formula for treating fish nocardia disease and a preparation method thereof, and relates to the technical field of aquaculture disease control, the formula contains five traditional Chinese medicine particles of cortex phellodendri, coptis chinensis, scutellaria baicalensis, mangnolia officinalis and liquorice, the total amount is 100g, the formula is matched with 20kg of feed, and the formula has strict requirements on particle size, content of effective components, purity and the like, and is suitable for large-scale production. The preparation method comprises the steps of raw material weighing and pretreatment, traditional Chinese medicine extraction and concentration, feed liquid mixing, medicinal bait low-temperature drying, finished product packaging and storage and the like, operation parameters of each step are specified in detail, and the effectiveness and stability of the formula are ensured; through a traditional Chinese medicine synergistic formula and strict quality control, fish nocardia disease is treated, the formula is complementary and synergistic, immunity is adjusted, chemical residues are avoided, green breeding is adapted, the preparation process is finely controlled, product consistency is ensured, the recovery rate of effective components is increased, the large-scale production requirement is met, disease loss is reduced, and the negative influence on the environment is reduced; and sustainable development of aquaculture is facilitated.