31 results about "Nocardia" patented technology

The invention relates to the technical field of bacteriophages, and provides a bacteriophage for fish nocardia and application of the bacteriophage, the bacteriophage is used for splitting fish nocardia, and the fish nocardia comes from a water body of a fish culture pond; the bacteriophage is preserved in China General Microbiological Culture Collection Center (CGMCC) on June 17, 2025, and the preservation number is CGMCC No.46495; the bacteriophage belongs to a tail bacteriophage, and the tail length is 165 + / -2nm. According to the technical scheme, the problem that at present, no bacteriophage for effectively treating and preventing fish nocardia exists is solved.

The invention belongs to the technical field of environmental engineering, and discloses a microbial agent for degrading malathion as well as a preparation method and application of the microbial agent. The microbial agent comprises the following raw materials: compound bacteria, a nutritional supplement and a biomass carrier material, wherein the compound bacteria comprise functional bacteria and synergistic bacteria, and the mass ratio of the functional bacteria to the synergistic bacteria is (1.2-1.8): 1; the functional bacteria comprise issatchenkia orientalis, nocardia, brevibacillus laterosporus and bacillus cereus; the synergistic bacteria comprise azospirillum brasilense, bacillus thuringiensis and lactobacillus buchneri. Functional bacteria with direct degradation capacity and synergistic bacteria with auxiliary functions are creatively compounded according to a specific proportion, meanwhile, the garlic straw powder is selected as a biomass carrier material, all the raw materials complement each other in function and have a synergistic effect, the degradation rate and thoroughness of malathion are remarkably improved, and the degradation efficiency of malathion is greatly improved. The defects of low degradation efficiency and poor environmental adaptability of a single strain are overcome.

The invention belongs to the technical field of disinfectant use evaluation, and particularly relates to an evaluation method for safe use of an aquatic disinfectant. The harmful bacteria (aeromonas hydrophila, vibrio harveyi, aeromonas veronii and nocardia) and beneficial bacteria are experimented, the harmful bacteria are used for exploring the concentration of the disinfectant, and the beneficial bacteria assist in verifying the reasonability of the concentration of the disinfectant, so that a proper disinfectant concentration range is obtained. The killing effect and the killing concentration of common pathogenic bacteria in aquaculture are explored through a common aquatic product disinfectant, meanwhile, the production concentration in the actual ecological environment is screened, and the appropriate and recommended use concentration of the aquatic product disinfectant is obtained. The method plays a scientific reference role in guiding reasonable use of the disinfectant and avoiding propagation of multiple drug-resistant bacteria.

The invention belongs to the technical field of biological detection, and relates to a nocardia detection kit and a detection method. The kit is based on the principle of PCR detection and comprises a primer pair used for amplifying the nocardia 16S rDNA gene, the sequence of a forward primer of the primer pair is shown as SEQ ID NO: 1, and the sequence of a reverse primer of the primer pair is shown as SEQ ID NO: 2. According to the nocardia detection kit and the detection method, the nocardia can be rapidly, sensitively and specifically detected on the basis of a PCR detection principle, and the nocardia detection kit and the detection method are particularly suitable for various application scenes such as clinical samples, environmental samples and scientific research samples.

The invention discloses a detection reagent, a kit and a detection method for pathogens of micropterus salmoides. The micropterus salmoides pathogen detection reagent comprises primers and probes for specifically amplifying an infectious spleen and kidney necrosis virus, a viral nervous necrosis virus, flavobacterium columnar, a perch rhabdovirus, a micropterus salmoides double-RNA virus, edwardsiella tarda, nocardia and a micropterus salmoides iridovirus; specific upstream and downstream primers and probes of the eight pathogens are sequences as shown in SEQ ID NO.1 to SEQ ID NO.24 in sequence. The micropterus salmoides pathogen detection reagent and method can detect eight pathogens at the same time, have the advantages of high sensitivity, high specificity, good repeatability and the like, provide reliable basis for prevention and control of the pathogens, also improve the prevention and control work efficiency, and have good application prospects. The kit is especially suitable for large-scale rapid screening and mixed infection diagnosis of aquaculture pathogens, and has important value and significance for prevention and treatment of micropterus salmoides pathogens.

The invention belongs to the technical field of biological bacterial fertilizers, and particularly relates to a high-salt-tolerance microbial liquid complex microbial inoculant suitable for saline-alkali desertification land and a biological bacterial fertilizer thereof. The microbial liquid complex microbial inoculant is prepared from the following functional strains by using electrochemical low-Hertz water through sequential inoculation and fermentation of three stages: salt-tolerant repair bacteria Nocardia halophila, salt-tolerant functional bacteria Bacillus subtilis and Azospirillum brasilense, nitrogen-fixing functional bacteria Azotobacter chroococcum, phosphorus-solubilizing functional bacteria Pseudomonas fluorescens, and nitrogen-solubilizing functional bacteria Pseudomonas fluorescens. The bacillus mucilaginosus is used as a potassium-dissolving functional bacterium, the trichoderma asperellum is used as a biocontrol growth-promoting bacterium, and the trichoderma harzianum is used as a rhizosphere optimizing bacterium. The biological bacterial fertilizer is prepared from the liquid complex microbial inoculant. Through scientific compatibility of different strains provided by the invention, efficient improvement and crop yield increase of the saline-alkali desertification land are realized, and the strain has important economic and social values.

The invention discloses a microbial agent for remarkably reducing organophosphate in compost as well as a preparation method and application of the microbial agent, and belongs to the technical field of microbial agents. The microbial agent comprises mycobacteria, nocardia-like bacteria and methylosporium, all the bacteria are mixed in the form of bacterial liquid, and the microbial agent comprises the following components in percentage by mass: 20 to 30 percent of mycobacteria, 30 to 55 percent of nocardia-like bacteria and 20 to 30 percent of methylosporium. The microbial agent is efficiently prepared on the basis of a specific culture medium through staged temperature regulation and control. The microbial agent can effectively reduce the content of organophosphate in compost and improve the safety and quality of the compost. The invention provides a novel solution for reducing the content of organophosphorus ester in the compost.

The application discloses a strain of completely degrading deoxynivalenol (DON), the N25 Nocardioides, the preservation number of the strain being CCTCC M 20252560. Average nucleotide identity (ANI) analysis shows that the strain is a new species in the Nocardioides genus, the strain can utilize DON toxin as a sole carbon source, and can completely degrade the DON toxin to form CO2 and H2O, completely eliminating the toxic effect, and can also degrade a plurality of fusarium toxins including A-type trichothecene toxin neosolensaric acid (NEO) and B-type trichothecene toxin 3-acetyl-DON (3-Ac-DON), nivalenol (NIV) and fusarenon-X (FUS-X). Therefore, the strain has a wide application prospect in the field of fusarium toxin detoxification.

The present invention discloses a strain of Nocardioides sp. and its application in the degradation of fungi toxins. The present invention screened and obtained a strain of Nocardioides sp. D-L4, which is a new species of the genus Nocardioides. The strain was deposited in the Guangdong Provincial Microbial Culture Collection Center on March 18, 2024, with a deposit number of GDMCC NO: 64435. The strain can efficiently degrade fungi such as DON, T-2 toxin and ZEN, and metabolize these toxins into low-toxic or non-toxic products. The metabolic activity of Nocardioides sp. D-L4 is stable and the reaction conditions are mild. It can be used in the preparation of fungal toxin detoxification, fungal toxin detoxification enzymes and fungal toxin detoxification engineered bacteria in the field of food and feed, and has good application potential.

The invention discloses a base fertilizer containing a microbial agent for brassicaceous plant broccoli, and belongs to the technical field of microbial fertilizers. The base fertilizer is prepared by the following steps: by taking pig manure and straws as organic raw materials, pretreating the organic raw materials, and mixing the pretreated organic raw materials with a mixture containing neurospora crassa, pseudonocardia alnus, bacillus, streptomyces, paenibacillus mucilaginosus, phanerochaete chrysosporium, azospirillum, trichoderma harzianum, pseudomonas fluorescens and thiobacillus thiooxidans; through staged fermentation, humic acid powder and a trace element mixture are added during aging, and finally bentonite is added for granulation and drying. The base fertilizer can improve the soil structure, adjust microbial communities, inhibit pathogenic bacteria, improve the nutrient utilization rate through nitrogen fixation, phosphate solubilization and potassium solubilization, provide comprehensive nutrition in combination with humic acid and trace elements, promote broccoli root development, enhance stress resistance and realize double improvement of yield and quality; the problems of soil degradation and environmental pollution caused by long-term use of chemical fertilizers are solved, and the requirements of green agriculture development are met.

The invention relates to the technical field of biological detection, and particularly discloses a real-time fluorescent PCR primer and probe combination and a kit for detecting nocardia. In the primers and the probe for detecting nocardia, the sequence of an upstream primer is shown as SEQ ID NO: 1, the sequence of a downstream primer is shown as SEQ ID NO: 2, and the sequence of the probe is shown as SEQ ID NO: 3. The real-time fluorescent PCR detection method for nocardia is established through the designed primer and probe combination, the method is high in specificity, nocardia can be rapidly and accurately detected from numerous pathogenic bacteria, the sensitivity is high, the lowest detection limit can reach 1 * 10 < 2 > copies / mu L, the accuracy is good, and the detection time is short. The method is more beneficial to early detection of subclinical cow mastitis caused by nocardia, early detection of diseases is realized, the loss of farmers is reduced, and the method has a wide application prospect.

The invention discloses a composition, a kit and a detection method for detecting pulmonary infection pathogens. The composition comprises at least one of a component A, a component B, a component C and a component D, the component A comprises a primer pair SEQ ID NO: 1-2 and a probe SEQ ID NO: 3; detecting bordetella pertussis by the component A; the component B comprises a primer pair SEQ ID NO: 4-5 and a probe SEQ ID NO: 6; the component B is used for detecting Nocardia; the component C comprises a primer pair SEQ ID NO: 7-8 and a probe SEQ ID NO: 9; detecting cryptococcus neoformans by the component C; the component D comprises a primer pair SEQ ID NO: 10 to 11 and a probe SEQ ID NO: 12; the component D is used for detecting acinetobacter baumannii. The composition for detecting the pulmonary infection pathogens provided by the invention can detect and distinguish at least one of bordetella pertussis, nocardia, cryptococcus neoformans and acinetobacter baumannii, has higher sensitivity, specificity and precision, can accurately and quickly detect bordetella pertussis, nocardia, cryptococcus neoformans and acinetobacter baumannii, and can be used for detecting the pulmonary infection pathogens. The detection efficiency is improved, the detection period is shortened, and detection is convenient.

The invention discloses a coloration LAMP (loop-mediated isothermal amplification) rapid detection method for nocardiasis of snakehead. The method comprises the following steps: (1) extracting a to-be-detected snakehead tissue DNA (deoxyribonucleic acid) sample; (2) adding the DNA sample into an LAMP reaction system, wherein the reaction system comprises a specific primer group, Bst DNA polymerase, dNTPs, a reaction buffer solution and a color indicator hydroxycalcein, wherein the specific primer group is designed aiming at a nocardia argus 16S rRNA gene conserved region; (3) reacting for 20 minutes at the constant temperature of 65 DEG C in 25 microliters of sample adding system; and (4) after the reaction is finished, if nocardia argus exists in the sample, the reaction liquid becomes green, and if nocardia argus does not exist, the reaction liquid becomes orange. The method is simple and convenient to operate and suitable for convenient and rapid detection in production. The whole LAMP detection time is shortened to 30 minutes, and the detection can be completed; compared with a commercially available kit, the speed is increased by 41.59%; the primers are high in specificity, the sensitivity of the primers is 10-100 times higher than that of conventional PCR, low-concentration pathogenic bacteria can be effectively detected, and the primers are suitable for on-site rapid detection and large-scale screening in nocardiasis of channa argus and fry production.

The invention relates to the technical field of biology, in particular to a reagent combination for identifying nocardia and application of the reagent combination. The invention provides an enzyme target, an enzyme target substrate, a coating solution and a kit for identifying eight different nocardia. The kit is used for identifying the eight nocard bacteria, the detection result can be read without depending on the growth of the bacteria only by preparing the bacteria into a bacterial suspension with a certain concentration and adding the bacterial suspension into a substrate for incubation, the sample treatment and operation are simple, the detection time is short, the result can be read manually or by a microplate reader, the cost of the required instrument is low, and the cost is low. The common clinical nocardia identification problem is effectively solved.

The invention discloses a preparation method and application of an organic waste fermentation inoculant, and belongs to the technical field of microorganisms. The organic waste fermentation inoculant is prepared from the following raw materials in parts by weight: 1 to 3 parts of bacillus subtilis, 2 to 4 parts of bacillus amyloliquefaciens, 2 to 3 parts of pseudomonas fluorescens, 4 to 6 parts of streptococcus thermophilus, 5 to 8 parts of lactobacillus delbrueckii subsp. Bulgaricus, 4 to 6 parts of nocardia albicans, 30 to 40 parts of porous activated carbon, 2 to 3 parts of beet extract, 4 to 6 parts of mannitol and 10 to 15 parts of straw waste powder. The organic waste fermentation inoculant can accelerate the degradation speed of organic matters in the composting process and shorten the fermentation time of the organic wastes, and has remarkable advantages in practical application.

The invention relates to the technical field of microbial control, and provides bacillus siamensis and application thereof, the bacillus siamensis has been preserved in China General Microbiological Culture Collection Center (CGMCC) on June 17, 2025, and the preservation number is CGMCC No.34929. The bacillus siamensis has been applied to the field of microbial control. According to the technical scheme, the problem that no bacillus siamensis is applied to prevention and treatment of fish nocardia disease in the prior art is solved. Research finds that the bacillus siamensis provided by the invention has a very good effect in the aspect of biological prevention and control of fish nocardia disease, and a new strain resource is provided for biological prevention and control of fish nocardia disease, so that the bacillus siamensis has a good practical application value.

The invention discloses a microorganism for preventing and treating clubroot of cruciferae and a preparation method and application of a fungicide of the microorganism, and relates to the technical field of biological prevention and treatment of plant diseases, the microorganism is screened out through a microbial metabolome and verified, the microorganism is matched with a microorganism resource library to form nocardia-like actinomycetes (1A6), and the use concentration of the microorganism is OD600 = 1. According to the microorganism for preventing and treating the clubroot of the cruciferae and the preparation method of the microbial inoculum of the microorganism, the strain is screened out from contrastive analysis of root swelling tissues of green vegetables and soil metabolome, and the microorganism is applied to preventing and treating the clubroot of the cruciferae, so that the clubroot prevention and control effect is effectively achieved; and the method has the advantages of low pollution and low residue.

The invention discloses a construction method of a fish nocardia disease pathogenic mechanism model, and belongs to the field of biotechnology and disease models. The invention aims to solve the technical problem that a deep causal mechanism is difficult to reveal due to the fact that microscopic dynamic interaction of pathogens and hosts cannot be observed in real time and in situ when an existing animal model and an in-vitro cell model are used for researching the disease. The method comprises the following steps: constructing a fish immune organ in an intelligent micro-fluidic chip integrated with a micro-actuator as a high-bionic-degree physical experiment platform; the living cell imaging system is used for collecting four-dimensional image data of the organoids in the nocardia infection process in real time. According to the method, through normal form transformation from passive observation to active intervention, the microscopic process of the disease can be reproduced in a high-fidelity manner and is regulated and controlled prospectively, so that a causal chain between key pathological events is deeply revealed, and a powerful intelligent research platform is provided for developing a new disease prevention and treatment strategy.

Use of red nocardia cell wall skeleton in the treatment of recurrent aphthous ulcer. The recurrent aphthous ulcer is selected from any one or combination of the following: mild recurrent aphthous ulcer, herpetic recurrent aphthous ulcer, severe recurrent aphthous ulcer. After administration, the ulcer can be observed to disappear, the hyperemia can be observed to disappear, and the pain symptoms can be observed to disappear.

The application discloses a primer probe combination, a kit and application for detecting Nocardia, a mycobacterium tuberculosis complex and mycobacterium, and belongs to the technical field of genetic engineering. The technical problem to be solved is that there is a lack of a nucleic acid detection tool for Nocardia, the mycobacterium tuberculosis complex and mycobacterium in the prior art, which is high in sensitivity, high in specificity and simple in operation. The technical solution is characterized in that the primer probe combination comprises a primer probe for detecting Nocardia, a primer probe for detecting the mycobacterium tuberculosis complex, a primer probe for detecting mycobacterium and a primer probe for detecting an internal standard control. The primer probe combination can simultaneously screen Nocardia, the mycobacterium tuberculosis complex and mycobacterium, quickly and simply confirms pathogenic species, saves time, manpower and reagent cost, and has the advantages of high specificity, a low minimum detection limit and prevention of false negative or false positive results.

The objective is to provide a wastewater purification solution for seafood processing plants that purifies wastewater, prevents sludge buildup, and prevents foul odors from being generated from the wastewater. [Solution] The solution is a mixture containing a culture medium obtained by culturing an aqueous solution of molasses with 10 species of bacteria from the genera Aspergillus, Bacillus, Lactopranthibacillus, Enterococcus, Nocardia, Pseudomonas, Ralstonia, Rhizopus, Rhodococcus, and Rhodosprillum, as well as iron fulvic acid, molasses, seaweed powder, and water.

The application provides a biomarker for identifying and detecting common pathogenic Nocardia, the biomarker is F6W96_34950, the biomarker is a nucleic acid sequence or an amino acid sequence, the nucleic acid sequence of the biomarker F6W96_34950 is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2. Through identification of the biomarkers, the application can accurately identify and detect Nocardia, and the process is simpler, faster, more accurate and lower in cost, so that at least partial differential diagnosis of diseases caused by Nocardia is realized, and the cure rate and prognosis are improved.

The application provides a set of biomarkers for identifying and detecting Nocardia, wherein the biomarkers are one or more of NCTC10797_02287, NCTC10797_01760, NCTC10797_05842, NOCYR_2299 and C5B73_13220, the biomarkers are nucleic acid sequences or amino acid sequences, the nucleic acid sequences of the biomarkers NCTC10797_02287, NCTC10797_01760, NCTC10797_05842, NOCYR_2299 and C5B73_13220 are shown in SEQ ID NO. 1-5, and the amino acid sequences are shown in SEQ ID NO. 6-10. By identifying the molecular markers, the Gelsenkirchen Nocardia can be accurately identified and detected, and the process is simpler, faster, more accurate and lower in cost, so that the differential diagnosis of diseases caused by the Gelsenkirchen Nocardia is at least partially achieved, and the cure rate and prognosis are improved.

The invention discloses an application of arginine in promoting micropterus salmoides to resist nocardia seriolae infection, which is characterized in that the arginine is used as a functional additive to feed the micropterus salmoides for 8 weeks, then a challenge experiment is carried out, and the influence of the arginine on the nocardia seriolae infection resistance of the micropterus salmoides is explored; comprising high-throughput omics conjoint analysis, mTORC1 pathway activity, antibody content, cell death, tissue structure, survival rate and the like. Researches find that arginine significantly activates an mTORC1 signal channel of micropterus salmoides, and promotes secretion of an IgM antibody in serum to resist nocardia seriola infection; the arginine also inhibits cell death caused by excessive activation of NLRP3 inflammasomes of the micropterus salmoides, so that the micropterus salmoides keeps complete liver, head-kidney and body-kidney structures after being infected by nocardia seriola, and finally the survival rate of the micropterus salmoides after being infected by nocardia seriola is improved. Therefore, the arginine can be used as a functional feed additive for enhancing the capability of the largemouth bass for resisting nocardia seriolae infection.

The invention discloses a combined flora SynCom4 capable of degrading fatty acid and application of the combined flora SynCom4. The combined flora consists of nocardia globulus DSM 44596, citrobacter fabricius CDC 2991-81, serratia marcescens NBRC 102204 and an eleostearic acid degrading strain 02 according to an equal proportion. When the combined flora is added into tung tree fruit compost, the content of fatty acid can be reduced, the content of total nitrogen, total phosphorus and total potassium can be increased, and the compost nutrient structure and fertilizer efficiency can be optimized; meanwhile, the fermented and decomposed tung tree fruit compost is applied to the tea garden soil, the soil microbial community structure can be improved, the soil micro-ecological stability can be improved, the soil fertility and sustainable utilization can be enhanced, collaborative development of tea garden ecological protection and high-quality planting is achieved, and the tea garden ecological protection and high-quality planting are promoted. An efficient and environment-friendly technical scheme is provided for resource utilization of tung tree fruit waste and ecological planting of a tea garden, and the method has important practical application value and popularization prospects.

The invention relates to the technical field of molecular biology, in particular to a primer probe combination product and application thereof. The primer probe combination product comprises two detection systems i) and ii), i) a primer and a probe for detecting mycobacterium tuberculosis, mucor and nontuberculous mycobacterium; and ii) a primer and a probe which are used for detecting pneumocystis yarrowii, nocardia and aspergillus and detecting cryptococcus. The primer probe combination product provided by the invention has good sensitivity and specificity, is simple and convenient to use, can simultaneously detect seven pathogens in two reaction systems, and can greatly improve the detection efficiency.

The present application discloses a Nocardia nematophila for biological control of root-knot nematodes and its application, which is the Nocardia nematophila strain PE06 with CCTCC NO: M 20221105. This strain and its metabolites can effectively control the root-knot nematode disease of cigar tobacco leaves, and have the characteristics of low use cost, high control efficacy and no residue. It can enrich the existing microbial species for controlling root-knot nematodes and has no pollution during application.

The present application belongs to the field of biological medicine and microbial culture technology, and particularly relates to a method for efficiently culturing red Nocardia and application thereof. The method for efficiently culturing red Nocardia comprises the steps of inoculating a red Nocardia fermentation strain into a fermentation medium and performing fermentation culture by using an air-lift fermenter. By optimizing the fermentation medium and using the air-lift fermentation culture mode with reduced shear force and improved mass transfer capacity to control air flow, a culture process suitable for high-yield cell mycelium of the strain is established, the synthesis capacity and cell amount of the red Nocardia mycelium cells are greatly improved, the harvested mycelium cells are mainly rod-shaped cells, which is more conducive to the crushing of the mycelium cells, the extraction and preparation steps of the cell wall skeleton are simplified, and thus a basis is provided for the large-scale preparation of N-CWS.

The invention relates to the technical field of Chinese herbal medicines, and provides a fermented Chinese herbal medicine composition for preventing fish nocardia disease and a preparation method, and the fermented Chinese herbal medicine composition comprises the following components in parts by weight: 30-40 parts of radix sophorae flavescentis, 15-20 parts of cortex fraxini, 8-10 parts of cortex meliae and 10-20 parts of bacillus amyloliquefaciens MS-2. According to the technical scheme, the problem that no fermented Chinese herbal medicine capable of effectively treating and preventing fish nocardia disease exists is solved. The fermented Chinese herbal medicine composition for preventing the fish nocardia disease, provided by the invention, has good prevention and treatment effects on the fish nocardia disease, can effectively prevent or treat diseases caused by fish nocardia infection in aquaculture, and greatly reduces the incidence probability of the fish nocardia disease.

The invention discloses a formula for treating fish nocardia disease and a preparation method thereof, and relates to the technical field of aquaculture disease control, the formula contains five traditional Chinese medicine particles of cortex phellodendri, coptis chinensis, scutellaria baicalensis, mangnolia officinalis and liquorice, the total amount is 100g, the formula is matched with 20kg of feed, and the formula has strict requirements on particle size, content of effective components, purity and the like, and is suitable for large-scale production. The preparation method comprises the steps of raw material weighing and pretreatment, traditional Chinese medicine extraction and concentration, feed liquid mixing, medicinal bait low-temperature drying, finished product packaging and storage and the like, operation parameters of each step are specified in detail, and the effectiveness and stability of the formula are ensured; through a traditional Chinese medicine synergistic formula and strict quality control, fish nocardia disease is treated, the formula is complementary and synergistic, immunity is adjusted, chemical residues are avoided, green breeding is adapted, the preparation process is finely controlled, product consistency is ensured, the recovery rate of effective components is increased, the large-scale production requirement is met, disease loss is reduced, and the negative influence on the environment is reduced; and sustainable development of aquaculture is facilitated.