The application discloses a primer probe combination, a kit and application for detecting
Nocardia, a
mycobacterium tuberculosis complex and
mycobacterium, and belongs to the technical field of
genetic engineering. The technical problem to be solved is that there is a lack of a
nucleic acid detection tool for
Nocardia, the
mycobacterium tuberculosis complex and mycobacterium in the prior art, which is high in sensitivity, high in specificity and simple in operation. The technical solution is characterized in that the primer probe combination comprises a primer probe for detecting
Nocardia, a primer probe for detecting the
mycobacterium tuberculosis complex, a primer probe for detecting mycobacterium and a primer probe for detecting an
internal standard control. The primer probe combination can simultaneously screen Nocardia, the
mycobacterium tuberculosis complex and mycobacterium, quickly and simply confirms pathogenic species, saves time, manpower and
reagent cost, and has the advantages of high specificity, a low minimum
detection limit and prevention of false negative or false positive results.