169 results about "Nocardia" patented technology

The invention provides a method for preparing an acid organic fertilizer by orientation fermentation of an organic waste. The method is characterized by comprising the following specific steps of: mixing bacillus subtilis, bacillus licheniformis, bacillus pumilus, saccharomyces cerevisiae, acetobacter aceti, trichoderma, aspergillus oryzae, nocardia and geotrichum candidum to obtain a first composite microbial bacteria agent; mixing the bacillus subtilis, the bacillus licheniformis, the saccharomyces cerevisiae, bacillus coagulans, lactobacillus acidophilus, lactobacillus plantarum, lactobacillus casei, pediococcus pentosaceus, the aspergillus oryzae, mucor racemosus and the nocardia to obtain a second composite microbial bacteria agent; adding the first composite microbial bacteria agentinto the organic waste and performing aerobic fermentation so as to form an intermediate fermentation product; and adding the second composite microbial bacteria agent into the intermediate fermentation product and performing anaerobic fermentation so as to form the organic fertilizer. The method has the advantages of capability of improving saline and alkaline lands, suitability for cultivation of acidophilous crops, effect of performing micro-ecological remediation on continuous cropping soil and low cost.

The invention discloses a multifunctional microorganism soil repairing agent as well as preparation and application thereof and belongs to the fields of biological techniques and environment protection. The multifunctional microorganism soil repairing agent is made of the following raw materials in parts by weight through uniform mixing: 30-60 parts of nano chitosan, 10-20 parts of humic acid, 10-20 parts of nano charcoal and 20-30 parts of a composite microbial agent, wherein the composite microbial agent is prepared from fresh benzene bacillus, B.sonorensis, lysinibacillus sphaericus, bacillus licheniformis, bacillus circulans, bBacillus thuringiensis, achromobacter denitrificans, saccharothrix australiensis, Rhodococcus zopfii, pseudonocardia sulfidoxydans and pseudonocardia dioxanivorans through purification, optimization, fermentation and drying dehydration. The multifunctional microorganism soil repairing agent disclosed by the invention is capable of replacing conventional physical and chemical repairing techniques, is capable of degrading complex pollutants, is wide in repairing range, is capable of preventing soil degradation and inhibiting diseases and insects in environments, and has the advantages of being good in repairing treatment effect, low in cost and economic and environment-friendly.

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

A slow-release solid chemical composition for environmental bioremediation is provided. The composition comprises a source of soluble organic substrates which include sugars, soluble organic polymers and mixtures of them in an amount of 7% to 90%, insoluble organic substrates an amount of 10% to 70%, complex inorganic phosphates in an amount of 0.5% to 7% and soluble organic salts in an amount of 2% to 70%. The insoluble organic substrates include fibrous plant materials, starches, cellulosic materials and mixtures of these substrates. The complex inorganic phosphates include ringed metaphosphates, linear polyphosphates and mixtures. The organic salts include lactates, formates, acetates, citrates, etc. Also the composition further comprises microorganisms which include Bacillus spp., Rhizobium spp., Bradyrhibzobium spp., Fibrobacter spp., Clostridium spp., Pseudomonas. spp., Geobacter spp., Arthrobacter spp., Nocardia, spp., aspergillus spp., Trichoderma spp., Candida spp., Yarrowia spp. and combinations of these microorganisms. The composition can be prepared in various forms, including granules, briquettes, pellets, tablets or capsules.

A method of enhancing plant growth comprises the step of introducing an alkane into a location adjacent to a plant. The alkane can be introduced intermittently, and can be combined with another gas and / or nutrients. The alkane preferably comprises a butane substrate. The butane substrate can stimulate the growth of butane-utilizing bacteria, such as Aeromonas caviae, Stenotrophomonas maltophilia, Micrococcus varians, Aureobacterium esteroaromaticum, Aureobacterium barkeri, Rhodococcus fascians, Nocardia paradoxus, Comamonas acidovorans and Pseudomonas aeruginosa. The alkane can increase the amount of heterotrophic bacteria in the location adjacent to the plant, and thereby accelerate a heterotrophic nitrification process. The butane substrate can also stimulate the growth of butane-utilizing fungi. The method can also enhance the growth protists and / or prokaryotes. A system for enhancing plant growth in accordance with the method is also disclosed.

The present invention relates to thermostable proteases having an amino acid sequence which homologous to the amino acid sequence of proteases derived from Nocardiopsis, and the production thereof by wild-type and recombinant host cells including transgenic plants and non-human transgenic animals. The proteases are effective in animal feed, in particular fish feed, and detergents. The proteases are capable of degrading the soybean Bowman-Birk inhibitor, and other antinutritional factors such as soybean agglutinin and the Kunitz trypsin inhibitor, as well as the isolated soy storage proteins glycinin and beta-conglycinin. Characteristic structural features of relevance for the thermostability of these proteases of peptidase family S2A or S1E are disclosed.

The invention provides bacillus velezensis and application of the bacillus velezensis as an aquatic pathogenic bacteria inhibitor. The bacillus velezensis is separated from the intestinal canal of healthy tilapia, and the preservation number of the bacterial strain is GDMCC No. 60344. The bacillus velezensis has a broad-spectrum antagonistic aquatic common pathogenic bacteria function, and has theadvantages that the growth of the pathogenic bacteria, such as streptococcus agalactiae, streptococcus iniae,nocardia seriolea, aeromonas hydrophila, aeromonas schubertii and edwardsiella tarda, canbe effectively inhibited, the use is safe, no residue is generated, reducing the usage amount and the residual amount of chemical drugs in aquaculture is facilitated, the production cost is reduced, and the practicality is strong.

Butane-utilizing bacteria are used to degrade hydrocarbon pollutants such as trichloroethene (TCE). In-situ or ex-situ techniques may be used to reduce or eliminate hydrocarbon pollutants from liquid, gas and solid sources. In a preferred embodiment, TCE concentrations in various aqueous environments are reduced by contacting a contaminated water source with butane-utilizing bacteria in the presence of oxygen to degrade the TCE by cometabolism or direct metabolism. Suitable butane-utilizing bacteria include Pseudomonas, Variovorax, Nocardia, Chryseobacterium, Comamonas, Acidovorax, Rhodococcus, Aureobacterium, Micrococcus, Aeromonas, Stenotrophomonas, Sphingobacterium, Shewanella, Phyllobacterium, Clavibacter, Alcaligenes, Gordona, Corynebacterium and Cytophaga. The butane-utilizing bacteria have relatively low TCE toxicity in comparison with conventional methane-utilizing bacteria, and demonstrate an improved ability to degrade TCE.

The invention relates to a novel microbial organic fertilizer with anti-actinomyces active compound bacteria and a preparation method thereof. The novel microbial organic fertilizer comprises a microbial inoculum and an organic fertilizer, wherein the microbial inoculum comprises colonies of Bacillus siliceous, Nocardia actinomyces, Bacillus subtilis, Bacillus thuringiensis, Brevibacillus laterosporus, azotobacter, Beauveria bassiana, Metarrhizium anisopliae, Trichoderma and Saccharomyces, and peptide proteins and rare-earth elements having unique effects on inhibiting wireworms and enhancing plant root system stress tolerancen; and the organic fertilizer comprises animal manure and plant fibers. The novel microbial organic fertilizer is prepared by fermenting the animal manure and plant fibers by using the microbial inoculum. The microbial organic fertilizer can quickly and effectively kill wireworm ova in soil, has strong effects on inhibiting and killing larvae and imagos, can continuously improve the growth environment of the plant root by using the growth of the microbial colonies and the stress tolerance of the peptide proteins, enables the plant to have strong roots, thick stems and green leaves, postpones the aging of the plant, and promotes prematurity and yield increase.

Described herein are formulations and methods for treating diseases caused by infectious pathogens, including mycobacterium avium complex, mycobacterium tuberculosis, Nocardia, Plasmodium falcium, and Plasmodium berghei.

The invention discloses a microbial agent for purifying sanitary sewage and a preparation method of the microbial agent. Active ingredients of the microbial agent is formed by mixing the following raw material bacteria including thiobacillus denitrificans, bacillus subtillis, bacillus licheniformis, bacillus polymyxa, lactic acid bacteria, nocardia amarae, denitrifying bacteria, rhodopseudomonas palustris, thiophilic red pseudomonas, rhodospirillum, saccharomycetes and propionibacterium. By means of the microbial agent, cellulose, saccharides, fat protein, pathogenic bacteria, viruses and parasitic ovum in sanitary sewage and pollutants, such as inorganic salt which is high in the contents of nitrogen, sulphur and phosphorus, the BOD and COD removal effect is guaranteed, meanwhile good bactericidal ability is achieved, high inhibiting ability is achieved especially for pathogenic bacteria, and the effect of treating sanitary sewage is remarkable.

The invention discloses a high-protein straw feed, which comprises crop straw powder, actinomycetes, nocardia, lactobacillus acidophilusis and bacillus subtilis, the weight ratio of the strains is 0.8-1.2:1.6-2.4:0.8-1.2:1.8-1.2, and the weight ratio of the total strain amount to the crop straw powder is 1:1,000-1,200. The preparation method comprises the following steps of: respectively screening, separating, purifying, rejuvenating and culturing various strains, transplanting the strains into an industrial fermentation tank for fermentation, mixing in a ratio, and adsorbing by using an adsorptive support; and inoculating the obtained composite viable bacteria powder into crop straw powder or a mixture of the crop straw powder, staking and fermenting at the temperature of between 60 and 70 DEG C, uniformly turning once every two days, and obtaining the high-protein straw feed after 30 to 35 days. The high-protein straw feed has the advantages of improving protein content, optimizing protein structure, improving the absorption and nutrient conversion rate of the straw feed, and improving the immunity of livestock.

The invention discloses a gene chip of aquatic product cultivation pathogenic bacterium, comprising a solid phase carrier which is modified chemically, a detection probe and a quality control probe are distributed on the solid phase carrier in a dot matrix way; the detection probe comprises specificity 16S rDNA sequences and / or gyrB gene sequences of vibrio, comma bacillus, vibrio harveyi, vibrio alginolyicus, vibrio anguillarum, vibrio parahemolyticus, nocardia, nacardia seriolea, aeromonas, hydrophilic aeromonas, streptococcus and dolphin streptococcus, which are to be detected, the quality control probe includes PCR positive, chip fixed positive control, chip hybridizing negative control, chip hybridizing positive control and chip hybridizing blank control; the gene chip has the advantages of small volume and high flux, can detect known and unknown germs of the vibrio, the nocardia, the aeromonas and the streptococcus, and can detect specific germs with multiple kinds, and the simpleness and rapidness and specificity of the germs can be detected, and automatic detection can be carried out after detection software is additionally arranged.

The preparation method of organic ecological soilless culture medium belongs to the field of agricultural bio technology. It is made up by using 100 portions of straw powder, 50-60 portions of cumulose fermented livestock and fowl dried excrements and 3-5 portions of compound bacterial preparation formed from azotogen and trichoderma preparation through the processes of uniformly mixing and stirring. The preparation of azotogen adopts cellulose Nocardia HD-86 with functions of decomposing cellulose and fixing nitrogen as strain and adds the corn stover, bran and water, and utilizes the aseptic fermentation process to obtain the azotogen, and the preparation of trichoderma preparation uses pseudo-Kan's trichodermin S28 as strain, and uses cob, bran, ammonium sulfate as culture medium through the processes of pulverizing, mixing, moistening, inoculating S28 and fermentation so as to obtain the trichoderma preparation. Said invention is favorable for industrial production of vegetables.

The invention relates to a method for treating or preventing (including immunising against) post-weaning multisystemic wasting syndrome (PMWS) and / or porcine dermatitis and nephropathy syndrome (PDNS) in a subject comprising administering an effective amount of a pharmaceutical composition or immune modulator composition comprising a whole cell of a bacterium from one or more of the genera Rhodococcus, Gordonia, Nocardia, Dietzia, Tsukamurella and Nocardioides, to said subject. In addition the method relates to the use of an immune modulator composition or a pharmaceutical composition comprising a whole cell of a bacterium from the genera Rhodococcus, Gordonia, Nocardia, Dietzia, Tsukamurella and Nocardioides, in the manufacture of a medicament for the treatment or prevention of post-weaning multisystemic wasting syndrome (PMWS) and / or porcine dermatitis and nephropathy syndrome (PDNS).

The invention relates to a compound microorganism bacterium agent used for sludge reduction, a preparation method and application of the compound microorganism bacterium agent. The compound microorganism bacterium agent is a liquid bacterium agent prepared by fermenting denitrified pseudomonas, nocardia coralline, candida utilis, rhodobacter sphaeroides and aspergillus sojae. According to the invention, the metabiosis action between microbe consortia is utilized to form a biodegradation system in which the activities of various required enzymes are all high, so dead or decrepit thalli in activated sludge can be effectively damaged and decomposed, and meanwhile, organic matters can be decomposed; and the generation quantity of excess sludge is reduced through reducing the quantity of the sludge in the process without influencing the quality of yielding water. On the premise of not greatly changing the traditional sewage treatment process, the sludge reduction ratio can reach 30%-70% in the sewage treatment process by adopting the compound microorganism bacterium agent, and the comprehensive running cost of the sludge treatment can be reduced, so that the compound microorganism bacterium agent is of great significance on the aspects of economic, environmental and social benefits.

Lovastatin, pravastatin, simvastatin, mevastatin, atorvastatin, and derivatives and analogs thereof are known as HMG-CoA reductase inhibitors and are used as antihypercholesterolemic agents. The majority of them are produced by fermentation using microorganisms of different species identified as species belonging to Aspergillus, Monascus, Nocardia, Amycolatopsis, Mucor or Penicillium genus, Streptomyces, Actinomadura, Micromonospora, some are obtained by treating the fermentation products using the method of chemical synthesis or they are the products of total chemical synthesis. The purity of the active ingredient is an important factor for manufacturing the safe and effective pharmaceutical, especially if the pharmaceutical product must be taken on a longer term basis in the treatment or prevention of high plasma cholesterol. The accumulation of the impurities from the pharmaceuticals of lower purity may cause many side effects during the medical treatment. The present invention relates to a new industrial process for the isolation of HMG-CoA reductase inhibitors using so-called displacement chromatography. Use of the invention enables one to obtain HMG-CoA reductase inhibitors of high purity, with high yields, lower production costs and suitable ecological balance.

The invention relates to a composite microbial agent for treating domestic garbage and a method for treating the domestic garbage by using the composite microbial agent. The composite microbial agent for treating the domestic garbage is prepared from the following components: brevendimonas diminuta, pseudomonas stutzeri, pseudomonas maltophilia, pseudomonas fluorescens, pseudomonas putida, candida utilis, candida lipolytica, schizosaccharomyces pombe, schizosaccharomyces octosporus, bacillus megatherium, bacillus subtilis, bacillus cereus, alcaligenes faecalis, clostridium beijerinckii, achromobacter denitrificans, nocardia coralline, and the like, wherein the parts by weight of the brevendimonas diminuta are 1-5, the parts by weight of the pseudomonas stutzeri are 1-5, the parts by weight of the pseudomonas maltophilia are 1-5, the parts by weight of the pseudomonas fluorescens are 1-5, the parts by weight of the pseudomonas putida are 1-5, the parts by weight of the candida utilis are 1-5, and the parts by weight of the candida lipolytica are 1-5.

The invention relates to a micro-organism preparation for preventing and curing streptococcicosis of Tilapia mossambica as well as a preparation method and an application thereof. The micro-organism preparation method for preventing and curing streptococcicosis of Tilapia mossambica comprises steps of compounding mixed vaccine with red Nocardia ATCC11653 and coral Nocardia ACCC40100, fermenting and cultivating the mixed vaccine with fermentation medium which is compounded of nitrogen source, carbon source and inorganic salt, and processing resultants into solid powder after the fermentation and cultivation. The micro-organism preparation for preventing and curing streptococcicosis of Tilapia mossambica, which provided by the invention, can reduce the content of ammonia nitrogen and nitrate nitrogen in the Tilapia mossambica breeding water body, antagonize the streptococcicosis of the Tilapia mossambica, reduce the accumulation of harmful substances of the middle and later breeding periods, enhance the immunity of the Tilapia mossambica,, achieve the aim of preventing and curing streptococcicosis of Tilapia mossambica, reduce the economic loss caused by diseases, and ensure the breeding output and quality of the Tilapia mossambica. The micro-organism preparation provided by the invention has advantages of convenience in use, small amount of usage and stable effect after use.

The invention discloses a formula of a water body remediation microbial inoculant and a preparation method of the remediation microbial inoculant, and particularly relates to the field of sewage treatment. The water body remediation microbial inoculant comprises a microbial agent and a carrier, wherein the microbial agent comprises nitrobacteria, denitrifying bacteria, fibrotic nocardia, lactobacillus plantarum, candida utilis, bacillus subtilis and bacillus mucilaginosus, and the carrier comprises wheat bran, zein and zeolite powder. Though adding the complex inoculantwhich is extremely easy in survival and reproduction into the water body remediation microbial inoculant, a beneficial dominant community is formed, and the ecological structure of microorganisms in a water body is regulated and controlled; the microbial inoculant can degrade and transform the harmful substances in polluted water, promote the growth of indigenous flora, and play a role in purifying water quality, more importantly, various microbial flora contained in the microbial inoculant can promote the growth of aquatic plants, prevent and control aquatic animal diseases, and inhibit excessive reproduction of algae, and finally the effect of stabilizing and restoring the ecological environment of the water body is achieved.

The present invention relates to a biological preparation, in particular, it is a red nocardia actinomycetes cell wall skeleton preparation. Firstly, culturing red nocardia actinomycetes CGMCC No.0712, making enrichment, culture, collecting thallus, breaking cell, enzyme purification, removing fat, blending, filling and freeze-drying so as to obtain the invented product containing red nocardia actinomycetes cell wall skeleton and dextran 40 according to a certain mixing proportion. The tests and clinical results show that its therapeutic effect is obvious for curing indications.

This invention relates to the isolation of thermophilic phytase-producing microorganisms, method for producing phytase using such microorganisms, phytase obtained therefrom, and usage of the phytase to hydrolyze phytic acid or phytate. In particular, this invention relates to phytase-producing microorganisms, which belong to Streptomyces sp., Pseudonocardia sp. or Microbispora sp., and which produce phytase available for recovery in an efficient and practical manner.

Lovastatin, pravastatin, simvastatin, mevastatin, atorvastatin, and derivatives and analogs thereof are known as HMG-CoA reductase inhibitors and are used as antihypercholesterolemic agents. The majority of them are produced by fermentation using microorganisms of different species identified as species belonging to Aspergillus, Monascus, Nocardia, Amycolatopsis, Mucor or Penicillium genus, some are obtained by treating the fermentation products using the methods of chemical synthesis or they are the products of total chemical synthesis. The present invention relates to the new amine salts of HMG-CoA reductase inhibitors, the preparation thereof, the preparation of pure HMG-CoA reductase inhibitors via amine salts thereof, use of the amine salts of HMG-CoA reductase inhibitors in the process for semisynthetic preparation of HMG-CoA reductase inhibitors, use of the amine salts of HMG-CoA reductase inhibitors in the process for biotechnological modification of HMG-CoA reductase inhibitors as well as the conversion of the amine salts of HMG-CoA reductase inhibitors into the pharmaceutically acceptable salts of the HMG-CoA reductase inhibitors and the conversion of the amine salts of HMG-CoA reductase inhibitors into the HMG-CoA reductase inhibitors in the lactone form.

A method of treating cancers by administering to a patient an anti-tumor effective amount ot at least one of a thermostable macromolecular antigen complex or a fragment of such a complex which is interspecific for microorganisms of the Mycobacteria, Nocardia and Corynebacteria group and which exhibits after immunoelectrophoresis an immunoelectrophoretic precipitation pattern corresponding to that of the antigen complex 60 of Mycobacteria bovis Calmette Guerin Bacillus strain. Preferably, the patient is also treated with an additional therapeutic agent which is specific against the patient's cancer such as a tumoral antigen or a non-proliferative tumor cell.

The invention discloses a microbiological bacterium and method for making same, wherein the microbiological bacterium comprises degreased saccharomycete, Trichoderma viride, high temperature bacillus, S. melanoferus, Nocardia and solid medium. The microbiological bacterium can be utilized as dejection treatment agent. Its preparation process comprises the three stage pure culture of these bacterial strains, and proportional mixing of the culture.

The invention discloses a microbiological preparation for efficiently treating municipal lake water pollution. The microbiological preparation is prepared from nitrosospira, rhodospirillum, bacillus subtilis natto, kitasatospora phosalacinea and lactobcillus in a volume ratio of 0-10 to 5-15 to 5-15 to 5-15 to 5-15. The microorganism selected and cultured by the invention has the capability of being rapidly multiplied and developed into a dominant microflora under the municipal lake water environment; and each strain can independently and cooperatively decompose eutrophic ingredients in the municipal lake water and can make certain eutrophic ingredients which are difficult to decompose in a normal state easy to decompose. The microbiological preparation has the advantages of purifying the water quality well, improving the substrate, optimizing the biological environment, saving the biological environment from damage, having obvious effect on water raising, water retention, water color stabilization, treatment of turbid water, water concentrate, red water, black water, bottom odourization and algae aging and regulation and stabilization of pH value of the water body and making the lake water clear and odorless rapidly, along with simple using method, low cost, obvious effect, no secondary pollution.

The invention discloses a detection primer set, detection kit and detection method for Vibrio parahaemolyticus, Photobacterium damsela and Nocardia seriolea. The primer set comprises a primer pair V.pa, a primer pair N.se and a primer pair P.da, and the nucleotide sequences of the primer pairs are disclosed as SEQ ID NO.1-6. The detection kit comprises the detection primer set. The detection method uses the detection kit and comprises the following steps: carrying out pretreatment on a sample, extracting genome DNA (deoxyribonucleic acid) as a sample set DNA, and detecting the sample by a PCR (polymerase chain reaction) process. The triplex PCR detection method established by the invention can be used for tracing detection on Vibrio parahaemolyticus, Photobacterium damsela and Nocardia seriolea in various stages, avoids transmission and prevalence of pathogens, and enhances the scientific management efficiency, thereby having very high practical value.

The invention discloses a sludge leavening agent. The sludge leavening agent is prepared from the following raw materials in parts by weight: 15-25 parts of bacillus subtilis, 10-20 parts of bacillus stearothermophilus, 8-15 parts of lactic acid bacteria, 5-10 parts of silicate bacteria, 4-10 parts of nocardia, 9-16 parts of a zotobacter chroococcum, 1-6 parts of saccharomyces cerevisiae, 1-5 parts of flavobacterium oryzae, 2-3 parts of photosynthetic bacteria, 1-3 parts of streptomyces microflavus, 6-13 parts of cellulase, 5-12 parts of amylase and 5-6 parts of xylanase. The sludge leavening agent provided by the invention is environmentally friendly, pollution-free, low in operation cost, small in floor space, and capable of effectively killing pathogenic bacteria, the parasites and the like in the sludge, degrading toxic and harmful substances, accelerating sludge deodorization and dehydration and decomposing nutrition ingredients which are difficult to absorb by crops.

The invention discloses a marine actinomycete strain with meloidogyne insecticidal activity and a preparation method and application of the marine actinomycete strain. The actinomycete strain is Nocardiopsis HA12420 preserved with the preservation number CGMCC No.11063 in China General Microbiological Culture Collection Center on July 9, 2015. The preparation method of an actinomycete fermentation broth includes the steps of seed culture, ferment culture and fermentation broth purification. The actinomycete fermentation broth has distinct insecticidal activity for meloidogyne incognita, meloidogyne javanica and meloidogyne arenaria and is applicable to prevention and control of meloidogyne of vegetables, flowers, melons, crops of orchards and the like, thereby having promising development prospect in agricultural production.