37 results about "Displacement chromatography" patented technology

The invention provides a low-temperature displacement chromatography hydrogen isotope separation device and a method. The invention makes the mixed gas of hydrogen isotope gas and helium after being cooled sequentially pass through a cooled main separation column and a cooled product gas collecting column which are filled with granulate palladium-loaded aluminum trioxide (Al2O3/Pd) to obtain the product gas rich in heavy isotope components of deuterium and tritium; a hot helium flow passes through and heats the main separation column so as to make the released gas sequentially pass through the cooled secondary separation column and the product gas collecting column which are filled with granulate palladium-loaded aluminum trioxide (Al2O3/Pd) to obtain the product gas rich in heavy isotopecomponents of deuterium and tritium; after the product gas is collected, middle rich gas flowing out from the secondary separation column is directly fed back into a raw material gas tank; and after the middle rich gas feedback process is accomplished, the main separation column and the secondary separation column are heated, and the gas released by heating is collected via a tail gas collecting column. The separation device has simple structure, reasonable separation process, and low cost for the construction and operation of the device. The invention has higher separation factor for hydrogen isotope separation.

The invention discloses an enrichment separation method of hydrogen isotopes through displacement chromatography. The enrichment separation method comprises the following steps: (1) reducing the temperature: making a molecular sieve column into a spiral coil, loading a container with liquid nitrogen, wholly immersing the molecular sieve column into the liquid nitrogen, and enabling the temperature of a molecular sieve to be maintained to be the temperature of the liquid nitrogen; (2) performing adsorption: enabling hydrogen to be introduced into the molecular sieve column, measuring the quantity of the hydrogen introduced into the molecular sieve, and after the gas pressure in the molecular sieve reaches to a definite pressure, stopping introducing the hydrogen; (3) performing warming for separation: upwards lifting the spiral molecular sieve column, so that the molecular sieve column is separated from the liquid surface of the liquid nitrogen, heating the molecular sieve column with air, and controlling the speed of the molecular sieve column being lifted out of the liquid surface of the liquid nitrogen to be 0.5-5 cm/min; and (4) performing detection and collection: oxidizing hydrogen into liquid water through an oxyhydrogen compositor at a rear end for the hydrogen discharged from the molecular sieve column, and then detecting the deuterium abundance in the liquid water through a magnetic mass spectrometer. According to the enrichment separation method of tritium through displacement chromatography disclosed by the invention, changes in the controlled process of reducing the temperature and raising the temperature have continuity, the hydrogen isotope separation efficiency is extremely high, and the separation effects are good.

The invention discloses a displacement chromatography hydrogen isotope separation device which comprises a rack as well as a cooling part, a separation column, an air supply part, an air collection part, a lifting part and a driving part. By adopting the displacement chromatography hydrogen isotope separation device disclosed by the invention, different adsorption functions that hydrogen and hydrogen isotope are replaced and separated by using a separation material can be achieved, the lifting part is driven by the driving part to precisely control the lifting position of the separation column, and then the separation column can be descended or lifted off the cooling part, so that adsorption or desorption of hydrogen isotope by the separation material under different temperature conditions can be achieved, a high retention rate of deuterium-tritium isotope in the separation material can be ensured, and a deuterium-tritium isotope gas obtained through final separation and selection is relatively high in purity; compared with the prior art, the device needs no parts for introducing deuterium or separating deuterium, meanwhile deuterium and tritium do not need to be replaced by introducing hydrogen after the separation material is in saturation adsorption of hydrogen, and in addition, the device is simple in structure, low in energy consumption rate, simple in process and low in cost.

A process for using L-isoleucine as the chiral selective agent to prepare the stationary phase for chiral ligand displacement chromatography is disclosed. Its advantages are simple operation (directly splitting the chiral substance in simple experimental condition), high analysis speed, and wide application range.

A preparation method of traceable enzyme calibration substance includes the followings: firstly, human-derived seroenzyme gene clone and expression; secondly, purification of high-purity gene recombination seroenzyme; thirdly, preparation of stroma ground substance with fine interchangeability; fourthly, interchangeability verification of calibration substance candidate; fifthly, homogeneity determination after subpackage; sixthly, freeze drying: the human-derived seroenzyme gene clone and expression are as follows: enzyme gene expression carrier Pet21b-HMCK specific construction process and protein optimization expression; the purification of high-purity gene recombination seroenzyme finishes high-purity protein of enzyme mainly by three reaction steps such as the first stage separation, anion displacement chromatography and the third molecular sieve gel chromatography; and then hybrid enzyme is determined and eliminated; the preparation method has the characteristics of reliable method, wide use, fine interchangeability and traceable performance, and the like.

The invention relates to an expression and purification method for bombyx mori odorant binding protein (BmOBP2). By designing a primer, an expression vector Pet-32a-BmOBP2 is constructed and recombined, a great quantity of recombined interest protein is obtained through inducible expression, and high-purity recombined protein can be obtained by carrying out two simple purification technologies namely nickel ion affinity chromatography and anion displacement chromatography on the recombined protein, so that the method lays a very important foundation for research of the three dimensional structure and biological function of the recombined protein, and the problem that the natural membrane protein has low expression quantity and is difficult to purify is solved.

A plurality of chromatographic separation operations, including a first simulated moving bed operation, are coupled into a process which functions, preferably through the application of continuous displacement chromatography, to recover a fraction rich in small organic molecules, notably betaine and/or invert from sucrose solutions, enabling the subsequent production of a high purity sucrose product.

A displacement chromatography process, including: loading onto a stationary phase comprising an anion-exchange material a mixture comprising one or more component to be separated; displacing at least one of the one or more component from the stationary phase by applying to the stationary phase a mixture comprising an polyaromatic polyanionic displacer compound having a general formula Cen (Ar)w, in which Cen is a central bond or group, Ar is an aromatic nucleus, w = 2 to the maximum number of sites one Cen, and Ar is substituted with a plurality of An<->, in which each An<-> is independently defined as sulfonate, carboxylate, phosphonate, phosphate, sulfate; and Ar is further substituted with a plurality of G, in which G is defined as independently H, C1-C6 alkyl, halogen, nitro, hydroxy, C1-C6 alkoxy. In addition, a group of polyaromatic polyanionic displacer compounds useful in the process is disclosed.

A process for separating organic compounds from a mixture by reverse- phase displacement chromatography, including providing a hydrophobic stationary phase; applying to the hydrophobic stationary phase a mixture comprising organic compounds to be separated; displacing the organic compounds from the hydrophobic stationary phase by applying thereto an aqueous composition comprising a non-surface active hydrophobic neutral zwitterionic displacer molecule and optionally an organic solvent; and collecting a plurality of fractions eluted from the hydrophobic stationary phase containing the separated organic compounds, in which the non-surface active hydrophobic neutral zwitterionic displacer molecule comprises a hydrophobic zwitterion having the general formula, as defined in the disclosure: [CM-R*-CM'].

The present invention relates to low acidic species (AR) compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods for producing such low AR compositions using displacement chromatography. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNFα is detrimental, are also provided.

Aminoglycoside-polyamines are disclosed along with methods of use thereof in displacement chromatography and as DNA-binding ligands. The aminoglycoside-polyamines are derivatives of carbohydrates, such as sugars, amino sugars, deoxysugars, glycosides, nucleosides and their substituted counterparts. The subject polyamines possess a

group in place of at least one hydrogen atom of at least one hydroxyl group of the carbohydrate compound. In these compounds R¹ is an alkyl group or an azaalkyl group, and R² is a primary or secondary amino group.

A process for separating organic compounds from a mixture by reverse-phase displacement chromatography, including providing a hydrophobic stationary phase; applying to the hydrophobic stationary phase a mixture comprising organic compounds to be separated; displacing the organic compounds from the hydrophobic stationary phase by applying thereto an aqueous composition comprising a non-surface active hydrophobic cationic displacer molecule and about 10 wt% or less of an organic solvent; and collecting a plurality of fractions eluted from the hydrophobic stationary phase containing the separated organic compounds; in which the non-surface active hydrophobic cationic displacer molecule comprises a hydrophobic cation and a counterion, CI, having the general formula A or B, as defined in the disclosure.

A sample is provided that can be purified by preparative reversed phase high performance liquid chromatography (Prep-RP-HPLC) in a single run in spite of recent advances in the production of reversed phase derivatized silica stationary supports: (1) The traditional approach is to use a bigger column (greater amount of stationary phase); and (2) Use displacement chromatography which (while labor intensive to develop) uses the stationary phase more effectively. This disclosure describes a unique Prep-RP-HPLC technique that uses a C-18/C-8 derivatized silica coated with a surfactant such as Triton X-100 to result in 7 to 10 fold increase in sample loading (of the crude mixture of organic compounds including synthetic crude peptides) in contrast to the conventional Prep-RP-HPLC technique. This increase in sample loading capacity and output is due to the additional surrogate stationary phase characteristic of the C-18/C8 adsorbed (bound) surfactant.