34 results about "Bioproducts" patented technology

The present disclosure provides compositions, kits, and systems comprising engineered bacteria and agricultural biologics, and methods of using the same to treat plants and improve one or more plant beneficial traits, such as plant yield, nutrient uptake, and resistance to abiotic or biological stress. Using the compositions, kits, and systems disclosed herein can further 5 reduce fertilizer usage. The present disclosure also proposes methods of treating plants using the engineered bacteria with agricultural biologics.

Methods and systems for monitoring and / or controlling production units used at various stages of biological product development and manufacturing are presented herein. In particular, in certain embodiments, the biological production monitoring and control techniques described herein utilize a mid-infrared (mid-IR) analyzer that is capable of obtaining mid-infrared spectral data from an aqueous sample in substantially real time. The techniques described herein can utilize this mid-infrared spectroscopic data to measure sample quality metrics, such as protein content, titer, secondary structure, aggregation, and the like, as well as viral and / or nucleic acid characteristics. Thus, sample quality metrics can be measured substantially in real time and / or continuously to assess, for example, the production quality of protein and gene therapeutic agents, and can be used by a control system to adjust and / or control process parameters to improve product quality, etc.

Described herein is an immortalized mammalian cell configured for production of a bioproduct of interest, which cell has a safe harbor locus selected from an Adeno Associated Virus 1 (AAVS1) locus, a CCR5 locus, and a Rosa26 locus into which a Human Papillomavirus (HPV) E6 gene sequence and an HPV E7 gene sequence have been inserted. Also disclosed herein are methods for immortalizing a mammalian cell for production of a bioproduct of interest as well as methods of producing a bioproduct of interest.

A method for controlling enzymatic hydrolysis of biomass-derived feedstock in a manufacturing process of a chemical bioproduct is disclosed. The enzymatic hydrolysis may take place in a hydrolysis reaction mixture. The hydrolysis reaction mixture may comprise solid matter. The method may comprise performing at least one Brix measurement on the hydrolysis reaction mixture; and controlling a dosing of at least one enzyme into the hydrolysis reaction mixture at least partially based on the results obtained from the at least one Brix measurement; wherein the results indicate a content of one or more soluble carbohydrates in the hydrolysis reaction mixture, and wherein the controlling of the dosing of the at least one enzyme into the hydrolysis reaction mixture is performed in order to affect at least the conversion of cellulose and optionally hemicellulose into monomeric carbohydrates in the hydrolysis reaction mixture.

Genetically transformed fungal strains for preparing a leather-like bioproduct and methods for obtaining said bioproduct, wherein the transformed strain has an increased carbohydrate hydrolytic activity as compared to the parent wild type strain and overexpressing genes such as swi6B, glsnf 1, gcn4, and / or nmnat.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a related biological product of an LhHB4 gene in regulation and control of accumulation of anthocyanin in plants. The nucleotide sequence of the LhHB4 gene is as shown in SEQ ID NO. 1, and the sequence of the encoded protein is as shown in SEQ ID NO. 2. Experiments show that the expression of the LhHB4 is in negative correlation with the biosynthesis of the anthocyanin of the lily, the accumulation of the anthocyanin in the lilies in the lilies can be remarkably inhibited by the instantaneous overexpression of the LhHB4 in the lilies, the accumulation of the anthocyanin in the lilies in the lilies in the leaves is promoted after the LhHB4 gene is silenced, and the regulation of the LhHB4 on the biosynthesis of the anthocyanin depends on an EAR motif. The related biological product of the LhHB4 gene provided by the invention provides important application support for cultivating new varieties with peculiar colors of lilies and improving the ornamental and commercial values of the lilies.

The present invention relates to an enzyme cocktail, a method for the deconstruction of lignocellulosic biomass using said cocktail, and a genetically modified microorganism for producing heterologous enzyme aimed at making biomass fermentable into biorenewable products such as biofuels, organic acids, and hydrocarbon.

This invention relates to a method for detecting defoamer 204, belonging to the field of bioproduct quality testing. This invention employs HPLC-ELSD to detect defoamer 204, providing a solution for determining the content of defoamer 204 in bioproducts by optimizing sample pretreatment conditions, HPLC parameters (mobile phase selection, elution gradient), and ELSD parameters (carrier gas flow rate, drift tube temperature, detector gain). Furthermore, this invention exhibits a wide linear range (R04 at 2.5~50 μg / mL) when used for defoamer 204 detection. 2 With advantages such as >0.99, high accuracy (recovery rate of 91.4~98.5%), high sensitivity (limit of detection of 1.0 μg / mL, limit of quantitation of 2.5 μg / mL), good specificity, and simple operation, it can accurately detect the presence and content of defoamer 204 in biological products, filling a gap in the field and having great significance for the quality monitoring and control of biological products.

The present disclosure relates to genetically modified host cells exhibiting reduced viscosity when cultured and uses thereof for production of bioproducts. The present disclosure provides genetically modified host cell comprising one or more genetic modifications that result in the downregulation of: (a) spaA or a homolog thereof, and (b) ptaB or a homolog thereof.

Microorganisms and bioprocesses are provided that convert gaseous C1 containing substrates, such as syngas, producer gas, and renewable H2 combined with CO2, into nutritional and other useful bioproducts.

The invention relates to the technical field of bioengineering, and particularly discloses a microorganism 3D printing embedding immobilization method based on double-network hydrogel. A microbial 3D printing embedding immobilization method based on double-network hydrogel comprises the following steps: heating and dissolving polyvinyl alcohol and sodium alginate in water, then adding a nano material, stirring and mixing uniformly, cooling, sequentially adding acrylamide, a cross-linking agent and a microbial agent, and mixing uniformly to obtain a first network premixed solution; dissolving calcium chloride in a saturated boric acid solution to obtain a solidification liquid; filling the first network premixed liquid into an extrusion type 3D printer, and immersing a printing head into the solidification liquid for printing to obtain a gel structure; taking out the gel structure from the solidification liquid, immersing the gel structure into a solution containing an initiator, and initiating polymerization to form stable dual-network hydrogel; and taking out the double-network hydrogel, and cleaning to obtain a final microorganism embedding product.

The use of renewable energy to reduce fossil fuel consumption is a key strategy to mitigate pollution and climate change, resulting in the growing demand for new sources. Fast-growing proprietary cyanobacterial strains of Fremyella diplosiphon with an average life cycle of 7-10 days, and a proven capacity to generate lipids for biofuel production were studied. We investigated the growth and photosynthetic pigmentation of a cyanobacterial strain (SF33) in both greenhouse and outdoor bioreactors and produced biocrude via hydrothermal liquefaction. The cultivation of F. diplosiphon did not significantly differ under suboptimal conditions (p<0.05), including in outdoor bioreactors with growth differences of less than 0.04 (p=0.035) among various batches. An analysis of the biocrude's components revealed the presence of fatty acid biodiesel precursors such as palmitic acid and behenic acid, and alkanes such as hexadecane and heptadecane, used as biofuel additives. In addition, the quantification of value-added photosynthetic pigments revealed chlorophyll a and phycocyanin concentrations of 0.0011±5.83×10−5 μg / μL and 7.051±0.067 μg / μg chlorophyll a. Our results suggest the potential of F. diplosiphon as a robust species that can grow at varying temperatures ranging from 13° C. to 32° C., while producing compounds for applications ranging from biofuel to nutritional supplements. This paves the way for production-level scale-up and processing of F. diplosiphon-derived biofuels and marketable bioproducts. Fuel produced using this technology will be eco-friendly and cost-effective and will make full use of the geographical location of regions with access to brackish waters.

The invention relates to the use of a lytic bacteriophage, stabilised in a carrier, of the family Myoviridae, Foxunavirus genus (deposit IDAC 261122-01), which acts as a biocontroller of Xanthomonas campestris pv. Campestris (Xcc), a phytopathogen responsible for the main disease affecting brassicas globally, angular leaf spot or black rot. In addition, the invention relates to the preparation of a bioproduct comprising the phage and an agronomically acceptable carrier, such as alginate and / or chitosan.

The present invention introduces innovative Multifunctional Food Ingredients (MFIs) designed to replace sodium nitrite in processed meats, fish and dairy. These MFIs are formulated from plant-based extracts of polyphenols, known for their antioxidant, and antipathogenic properties, efficacy in food preservation, colour stabilisation, and a broad spectrum of health advantages, further enhanced by dietary fibre. This invention directly addresses public health concerns linked to the consumption of nitrite food additives and their associated risks of colon cancer and cardiovascular diseases. Furthermore, the synergistic combination of polyphenols and dietary fibres provides enhanced benefits for gut health, marking a significant advancement in the formulation of food additives. Additionally, this invention aligns with the global shift towards sustainable, eco-friendly bioproducts, contributing to the reduction of agri-food waste.

Microorganisms and bioprocesses are provided that convert gaseous C1 containing substrates, such as syngas, producer gas, and renewable H2 combined with CO2, into nutritional and other useful bioproducts. Disclosed herein are microorganisms containing that are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. The application describes methods for production of glucose and the glucose polymer starch and other polysaccharides by CO2-fixing chemoautotroph strains. The invention includes a method of production wherein the organisms are cultivated microaerobically or aerobically in a bioreactor using CO2, H2 and O2 in an oxyhydrogen fermentation and methods for recovery and purification of secreted glucose, intracellular starch, and other polysaccharides.

The disclosure relates to novel methods, compositions, and genetically modified microorganisms for extracting and / or isolating bioproducts from microorganisms having recalcitrant cell walls. In some aspects, the disclosure relates to solvent-free methods of extracting and / or isolating bioproducts. The disclosure further relates to bioproducts having less than 10 ppm of a solvent.

The invention relates to a method of transferring a microbial bioprocess from a multi-use bioreactor (1) to a single-use bioreactor (2), wherein the source bioprocess is a batch or fed-batch microbial bioprocess and the source bioprocess arrangement (3) comprises a source bioreactor (5) with a stirrer and the source bioreactor (5) is the multi-use bioreactor (1) which has at least 1000 L production volume of a microbial culture producing a maximum heat of at least 25,000 W and / or the stirrer of the source bioreactor (5) is driven with a maximum torque of at least 120 Nm, wherein the target bioprocess is a continuous microbial bioprocess and the target bioprocess arrangement (4) comprises a target bioreactor (6) and the target bioreactor (6) is the single-use bioreactor (2) which has a production volume of a microbial culture of at most 50 % of the production volume of the source bioreactor (5) and of at most 800 L, the microbial culture of the target bioreactor (6) produces a maximum heat of at most 20,000 W during the target bioprocess and / or the target bioreactor (6) comprises a stirrer driven with a maximum torque of at most 100 Nm, wherein an average amount of the bioproduct produced per time of performance of the target bioprocess by the target bioreactor (6) is at least 90 %, preferably at least 100 %, of an average amount of the bioproduct produced per time of performance of the source bioprocess by the source bioreactor (5).

The utility model discloses a microalgae and anaerobic fermentation integrated biomass energy production circulating system which comprises an IC anaerobic tower reaction generator, a circulating water tank, a T-shaped pipeline, a circulating water pump, a main pipeline, a microalgae collecting port and a microalgae circulating incubator, and the IC anaerobic tower reaction generator is connected with the circulating water tank; the T-shaped pipeline is connected with the circulating water tank; a microalgae collecting opening is formed in one end of the T-shaped pipeline, and the other end of the T-shaped pipeline is connected with the main pipeline through a circulating water pump; a water inlet of the microalgae circulating incubator is connected with the main pipeline, and a water outlet is connected with the circulating water tank. According to the utility model, the effects of wastewater purification and biomass energy production are realized through organic combination of all the devices, anaerobic fermentation treatment is carried out through the IC anaerobic tower reaction generator, and liquid generated by anaerobic fermentation is purified through microalgae in the microalgae circulation culture device; finally, the purposes of efficiently treating high-organic-concentration wastewater and obtaining energy and biological products with high additional values are achieved.

This invention relates to the field of biotechnology, specifically to the application of bioproducts expressing the EsPTI15 gene to regulate tiller number. The nucleotide sequence of the EsPTI15 gene is shown in SEQ ID NO:1. This invention is the first to discover that, compared to wild-type *Leymus chinensis*, EsPTI1… 5 Overexpression of the EsPTI15 gene significantly increased the number of tillers in *Triticum aestivum*, confirming that overexpression of the EsPTI15 gene positively regulates tillering development. This finding not only enriches the molecular mechanism theory of plant tillering regulation but also demonstrates the potential of the EsPTI15 gene to increase the number of tillers, paving the way for subsequent gene manipulation to introduce EsPTI1. 5 The research and development of technologies that increase the number of tillers in forage plants and thus improve biomass has provided key theoretical support and genetic resources.

Disclosed is a composition comprising (a) a lignocellulosic material and / or a cellulosic material; and (b) a cellulose derivative. A process for preparing the composition is also disclosed. The process can comprise providing a cellulose derivative in a solvent; and mixing a lignocellulosic material and / or a cellulosic material into the solvent. The lignocellulosic material and / or the cellulosic material can comprise 90% of particles ranging from 0.01 to 5 mm in size. The lignocellulosic material and / or the cellulosic material can be derived from a biomass residue.

The present disclosure generally relates to a method of making a biological product (e.g., an enzyme) using fermentation and subsequent purification of the biological product. By utilizing online biomass data for feedback control in the fermenter, efficient and stable cross flow microfiltration operations can be performed with active cells.

Provided herein are compositions and methods for engineering a bacterium comprising a reduced capacity for cellulose formation, a reduced capacity for foaming behavior, or an increased capacity for cell density for enhancing bioprocessing outcomes associated with the fermentative production of industrially relevant bioproducts.

A catalyst free organosolv process for a more efficient delignification and bio-product production by fractionating lignocellulosic materials comprises of providing one or more lignin dissolving chemicals with water and placing it in contact with lignocellulosic biomass. The process also includes producing used solvent as a result of placing biomass in contact with lignin dissolving chemicals and water, separating it into organic and aqueous layers, extracting poly lignin from the organic layer to produce recyclable lignin dissolving chemicals, and extracting dissolved solid from the aqueous layer to produce recyclable water. The catalyst free organosolv process can use any combination of fresh, used, or recyclable water and lignin dissolving chemicals, with or without any pH adjustment and placing them in contact with any biomass in process, or any new biomass. The recyclable lignin dissolving chemicals can be further separated to remove any excess or desired organic chemicals from it. The separated chemicals can be recycled as new lignin dissolving solvent or sold, while poly lignin can be used as a natural and renewable colorant for other polymers such as poly lactic acid, or can be mixed with other polymers as an additive and be compounded, co-extruded, injected to make consumable polymeric parts, or can be used as a natural source of renewable aromatics. Application of the catalyst free organosolv process helps to preserve fiber strength which is important for certain applications. At the end, the catalyst free organosolv fractionation process produces good quality fibers, various organic chemicals, and poly lignin that together make the process very economically attractive.

Provided herein are compositions and methods for engineering a bacterium comprising a reduced capacity for cellulose formation, a reduced formation, a reduced capacity for foaming behavior, or an increased capacity for cell density for enhancing bioprocessing outcomes associated with the fermentative production of industrially relevant bioproducts.

A method for controlling a pretreatment part in a manufacturing process of chemical bioproducts comprises measuring at least one colour coordinate value of a product that has undergone a hemihydrolysis reaction and steam explosion as parts of said pretreatment part of said manufacturing process. A decision rule is applied to make a control decision at least partly on the basis of the at least one measured colour coordinate value. One or more control actions are implemented in one or more process steps of said pretreatment part in accordance with said control decision.

The present invention relates to the direct use by addition of drilling gravels residue from the oil exploration and production activities in the formulation of culture medium of unicellular organisms, increasing its growth rates, intensifying the CO2 biofixation, and generating value to this residue from of productivity gains expected by autotroph and mixotroph organisms (cyanobacteria, microalgae and macroalgae), as well as the production of bioproducts that can be generated through the concept currently described in the literature as biorefining. Algae are cultured using drilling gravel suspended in the culture medium together with the ability to grow by absorbing CO2. Mechanisms used by algae in soils and marine environments to tolerate salinity, sodicity and contamination of petroleum hydrocarbons provides wide adaptation to these conditions of abiotic stress and enables the destination without environmental impact, constituting a satisfactory solution for the destination of the gravel for oil exploration and production.

Provided are genetically modified strains of Aureobasidium pullulans that can produce a bioproduct which is heavy oil composed of polyol fatty acid esters (glycolipids, liamocin) with increased productivity and a method of producing a bioproduct using the same, wherein the genetically modified strain of Aureobasidium pullulans comprises an overexpression of a functional VHb protein and an overexpression of a functional PacC protein and is grown under conditions required to support the production of the bioproduct to collect the bioproduct in a high yield.

The present invention relates generally to the generation of bio-products from organic matter feedstocks. More specifically, the present invention relates to improved methods for the hydrothermal / thermochemical conversion of lignocellulosic and / or fossilised organic feedstocks into biofuels (e.g. bio-oils) and / or chemical products (e.g. platform chemicals).

A system and process for producing pulp and other bioproducts from non-wood raw materials are described. The non-wood raw materials are screened, desilicated, impregnated, mechanically refined to obtain a pulp stream, and then subjected to oxygen-alkali treatment. Lignin and silica are separated from the pulp stream to obtain pulp. Desilication is carried out at a first temperature using warm water, an alkaline solution, and / or an alkaline liquor with a low alkali charge to selectively separate silica from the non-wood raw materials. Impregnation is carried out at a second temperature using an alkaline solution with a low alkali charge to selectively separate lignin from the non-wood raw materials and prepare the pulp for mechanical refining. The oxygen-alkali treatment is carried out at a third temperature to separate more lignin from the pulp stream.

The invention relates to the technical field of microbiological culture, and discloses a periphyton efficient culture system and a water purification method.The periphyton efficient culture system comprises an environment regulation and control module, a dynamic nutrition supply module, a water purification module, a water purification module and a control module, the high-adhesion biological membrane support module is used for improving the stability and adhesion of a biological membrane, the low-energy-consumption culture module is used for improving renewable energy sources and a sewage recycling technology, the ecological competition module is used for introducing functional strains, a community structure is regulated and controlled through biological competition, and the self-repairing ecological feedback module is used for sensing and feeding back to realize community abnormity recognition and automatic repairing. The whole-process monitoring and optimization module is fused with an AI algorithm and supports remote sensing and system optimization, and the low-carbon resource recovery and biological product conversion module is used for capturing and fixing CO2 by using periphyton. Based on an environment regulation and control and ecological feedback dual-core mechanism, the system realizes accurate regulation of environment parameters, stable maintenance of biocenosis and minimization of manual intervention, and an efficient and stable artificial ecological system is constructed.