The application discloses a preparation method of a material for enriching hydrophilic
biological substances, and the core of the method is to perform
peptide segment enrichment through specific binding of chemically modified
magnetic nanoparticles and glycosylated
peptide segments, so that the problems of high cost, complicated operation and limited identification
throughput are solved, and an efficient, stable and easy-to-operate technical process is provided for
glycoproteomics research.
High selectivity: the surface of the magnetic beads is a combination of mesoporous structures and
quaternary ammonium salt groups, which significantly improves the selectivity of the glycosylated
peptide segments and reduces non-
specific adsorption. The number of N-glycans can be identified to an average of 2733, and the number of O-glycans can be identified to an average of 1777. Simple operation: the introduction of the magnetic beads simplifies the enrichment process and facilitates subsequent automated experiments and large-scale applications. Cost reduction: by simplifying the synthesis method and improving the stability of the material, the preparation and application cost of the material is reduced.