143 results about "Active matter" patented technology

The invention discloses a near-infrared fluorescent probe for detecting ONOO <-> as well as a preparation method and application of the near-infrared fluorescent probe, and belongs to the technical field of peroxynitroso ion detection. The near-infrared fluorescent probe for detecting ONOO <-> provided by the invention is high in detection speed, and the highest fluorescence intensity can be reached within 60 seconds; the reaction is obvious and easy to observe, when the compound reacts with ONOO <->, strong red fluorescence can be generated, and the change of the color of the solution can be observed by naked eyes; the selectivity is strong, the sensitivity is high, the interference of other biological molecules and active substances can be effectively avoided, the accuracy and the reliability of a detection result are ensured, and the lowest detection limit can reach 107nM; the hydrogel has good biocompatibility, is low in toxicity in a living body, and does not generate adverse effects on normal physiological functions of a biological sample; the provided preparation method is simple and easy to operate, the raw materials are low in cost and easy to obtain, the synthesis efficiency is high, and the preparation method can be used for large-scale industrial production and has wide application prospects.

The invention discloses a preparation method and application of anti-photoaging collagen polypeptide. The preparation method comprises the following steps: unfreezing and cleaning silver carp skin, airing and cutting; naHCO3 is added, and soaking is carried out; adding Na2CO3 and carrying out ultrasonic impurity removal; performing ultrasonic-assisted treatment; adding protease for enzymolysis treatment; establishing an enzymolysis kinetic model under the alkaline protease condition; standing, cooling and centrifuging the enzymatic hydrolysate, collecting supernate, filtering, and performing vacuum freeze drying to obtain faint yellow SCSCP; the light aging resistant peptide SCSCP02 is separated, and the active peptide sequences of the light aging resistant peptide SCSCP02 are GPPGPPGTPGPQ, SGLPGPIGPPGPR and GLPGPIGPPGPR. The active substances which are extracted from the fish skin and have the anti-photoaging effect can reduce the risk of skin light damage caused by UVR by acting on various treatment targets, resource waste can be reduced, environmental pollution caused by biological resources can be relieved, and sustainable development of the industry can be promoted.

The invention relates to a multi-active particle size gradient multilayer dry-method thick electrode and a preparation method thereof, the structure of the dry-method thick electrode comprises a current collector, the current collector is provided with a first surface and a second surface which are oppositely arranged along the thickness direction, the first surface and the second surface are sequentially provided with a plurality of layers of dry-method electrode diaphragms from inside to outside, and the dry-method electrode diaphragms are arranged on the first surface and the second surface. The particle sizes of active substances in each layer of diaphragm of the multi-layer dry-method electrode diaphragm are different; the preparation method has the advantages that the electron / ion conductivity of the pole piece is improved, and the rate capability of the pole piece can also be improved.

The invention provides a composite nanoparticle with active substance sequential release capability, a multifunctional microneedle patch based on curcumin-loaded ZIF-67 nanoparticles, preparation methods of the composite nanoparticle and the multifunctional microneedle patch and application of the multifunctional microneedle patch, and belongs to the technical field of biology. The composite nano-particles with the active substance sequential release capability are CurZIF-67NPs, the average particle size is 403 + / -7 nm, the microstructure is dodecahedral structure particles with rough textures on the surface, and the composite nano-particles have the effects of slowly releasing bioactive substances, improving the inflammatory microenvironment and regulating and controlling osteogenesis under certain conditions. The composite nano-particle comprises curcumin powder and a ZIF-67 nano-material, the ZIF-67 nano-material can be decomposed to quickly release Co < 2 + >, macrophages are regulated and controlled to be polarized to an M2 type, and excessive immune inflammation reaction is improved; the curcumin powder is slowly released, so that the periodontal ligament stem cells can be regulated and controlled to be differentiated into osteoblasts, and the osteogenic activity is enhanced.

The invention relates to a preparation method of a blocky solid defoaming agent, which comprises the following steps: premixing a defoaming active matter and an emulsifier, stirring and mixing with paraffin at a certain temperature, and cooling and molding the mixed material to obtain the blocky solid defoaming agent. Wherein the emulsifier can ensure that the defoaming active matter can be uniformly dissolved and dispersed in a foaming system, so that an excellent defoaming effect is achieved. Paraffin is relatively high in hydrophobicity, so that not only can the performance of the product be enhanced, but also the blocky product can be ensured to realize a long-term foam eliminating and inhibiting function in flowing foaming liquid.

The invention provides kelp fucoidin with low molecular weight and high anti-anaphylaxis as well as a preparation method and application thereof, and belongs to the technical field of food processing. The preparation method comprises the following steps: mixing kelp fucoidin with water to obtain a kelp fucoidin solution; and mixing the kelp fucoidin solution with H2O2 and Vc, reacting, adjusting the pH value, dialyzing and freeze-drying to obtain the kelp fucoidin with low molecular weight and high anti-allergic property. The H2O2-Vc degradation system is innovatively applied to preparation of the low-molecular-weight kelp fucoidin and improvement of the anti-allergic activity of the low-molecular-weight kelp fucoidin. In the degradation process, the chemical composition of the fucoidin is partially changed, but monosaccharide types and functional groups are not influenced. Through multiple ways of cell model and animal model experiments, the degraded laminaria fucoidin is more prominent in anti-allergic activity. Technical support is provided for development of seaweed-derived anti-allergic functional active substances, and high-valued utilization of kelp resources in the fields of functional food and medicines is further promoted.

The invention discloses a rhodotorula mucilaginosa strain as well as a fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms. The rhodotorula mucilaginosa BTN-HB-L28 strain is separated and purified from meconopsis in Lijiang of Yunnan, can be used in the field of cosmetics, has the effects of resisting skin oxidation, resisting aging, repairing skin and relieving skin, can be used as an active matter in skin care products, and has practical application value. The fermentation product filtrate of the strain has excellent HAS inhibition ability, and can significantly inhibit the expression of matrix metalloproteinase 3; the fibroblast proliferation can be obviously promoted under various concentrations; the proportion of SA-beta-Gal positive cells can be remarkably reduced under various concentrations, and the anti-aging effect is achieved; the antioxidant composition has remarkable DPPH and ABTS free radical scavenging capacity and has a good antioxidant effect; the secretion amount of cell inflammatory factors NO, IL-6 and TNF-alpha can be remarkably inhibited, and the good relieving effect is achieved.

The invention discloses a method for screening an active matter for protecting skin cell telomeres, and relates to the technical field of biotechnology and cosmetic development, and the method is technically characterized by comprising the following steps: a) providing stem cells derived from an exosome which carries at least one microRNA for regulating and controlling a telomere protection factor CTC1; b) acting a candidate active matter on the stem cells, and separating to obtain a treated exosome; the technical effects are as follows: the combination of miR-150-5p and miR-181c is delivered through the exosome, the dual-miRNA coordinated regulation is realized, the limitation of the traditional single-target detection is broken through, the CD63 / CD81 protein on the membrane surface of the exosome can be specifically combined with the skin cell membrane, and the Franz diffusion experiment verifies that the micro-fluidic chip integrates the collagen scaffold and the dynamic shear force.

The invention discloses a lamellar liquid crystal nano composition with multiple anti-aging effects and application thereof, and the lamellar liquid crystal nano composition comprises the following components in percentage by total weight of the composition: 0.1-20% of glycolipid emulsifier; 0.1%-5% of an amino acid emulsifier; 0.1%-5% of retinol and a derivative thereof; 0.01%-5% of a water-soluble active matter; 0.01% to 1% of ceramide NP; 10%-20% of grease; 30%-50% of a polyol; the total percentage is 100%. A biological surfactant, ceramide and an amino acid surfactant are compounded according to a certain proportion to form a lamellar liquid crystal structure similar to a skin structure.

The present disclosure provides lipid nanoparticles having a sphere like structure, compositions comprising the lipid nanoparticles and methods for delivery of the agents. The lipid nanoparticles have improved properties for delivery of biologically active agents, such as RNA.

The invention relates to a preparation method of a CpG ODN nano immune complex, which comprises the following steps: (1) uniformly mixing a protamine solution with a certain concentration and a CpG motif-containing oligodeoxynucleotide CpG ODN aqueous solution with a certain concentration according to a certain ratio, and carrying out electrostatic compounding assembly at room temperature to form a suspension; and (2) applying energy to the suspension to disperse / homogenize uniformly to form the suspension of the nano-composite. The nano-composite prepared by the method disclosed by the invention is controllable in particle size and controllable in surface charge. The nano particles formed under the preparation condition of an optimized proportion are uniformly dispersed, good in stability, good in resolubility after freezing, and good in long-term storage stability. The preparation method provided by the invention is simple, does not involve toxic and harmful chemical substances, and is suitable for amplification. Compared with the prior art, the preparation method provided by the invention can obviously improve the effective concentration of the effective active substance CpG ODN in the nano immune complex. The invention also relates to the nano immune complex prepared by the method and application thereof. Cell and animal experiments prove that the nano immune complex prepared by the method has good immunostimulatory activity, can stimulate the maturation of related immune cells and induce the secretion of related cytokines, can be independently used as an immunomodulator, and can also be used as an immunologic adjuvant to be combined with various antigens to be used as a vaccine preparation as a whole. The nano immune complex has good application prospects in the fields of inducing and activating immune cells to mature, releasing cytokines, resisting allergic diseases, preventing and treating microbial infectious diseases, vaccine adjuvants, resisting tumor immunotherapy and resisting Alzheimer's disease.

The invention relates to an acellular matrix material with biological functional activity and a preparation method thereof. The preparation method comprises the following steps: 1) providing an acellular matrix material subjected to crosslinking treatment; 2) reacting the crosslinked acellular matrix material with a biocompatible polymer, wherein the biocompatible polymer has a reactive group and a first subject and / or guest molecule; the reactive group is covalently bonded to a functional group on the acellular matrix material; and 3) reacting the acellular matrix material obtained in the step 2) with a second subject and / or guest molecule modified by a biological functional active substance through subject-guest interaction. Through the mode, the anti-calcification performance and the biological activity of the acellular matrix material can be improved at the same time, and the acellular matrix material is flexibly endowed with various biological function activities.

A system and method for removing a target substance in a surface or subsurface water flow includes an enclosure formed by a geotextile wrapping material, and a filling composition contained in the enclosure. The filling composition includes an amount of active material dispersed in a media material, where the active material is capable of binding, absorbing or otherwise reacting to the target substance to thereby reducing the amount of the target substance from the resulting water flow exiting the system.

A preparation method and an application for an oil-in-water nanoemulsion material are provided, the method is a new method combining sonication-phase inversion composition-phase inversion temperature, S-PIC-T method, i.e., the combined application of the sonication, the phase inversion composition method and the phase inversion temperature develops a new type nano-preparation with small droplet size, high loading hydrophobic small molecule substances, and stable physical and chemical properties. Therefore, in the present disclosure, the sonication in the high-energy method is combined with PIC and PIT in the low-energy method, because the sonication does not produce high heat, the amount of surfactant is reduced, and the size of emulsion droplets is reduced while the energy consumption is low, while PIC and PIT reduce production costs and protect the biological activity of hydrophobic bioactive substances.

The invention discloses a method for extracting a natural antioxidant from a cyperus esculentus shell fermentation product by using subcritical water, and belongs to the technical field of plant antioxidant extraction. The preparation method comprises the following steps: fermenting cyperus esculentus shell powder to obtain fermentation liquor; performing subcritical water extraction on the fermentation liquor to obtain an extracting solution; and filtering, purifying and freeze-drying the extracting solution to obtain the natural antioxidant. The plant cell walls can be selectively and mildly degraded and deconstructed by utilizing rich enzyme systems secreted by specific microorganisms in the growth and metabolism process. The biological pretreatment not only can destroy the physical barrier of the cell wall, but also can release active substances in a combined state in the cell wall, so that the active substances are converted into a free state, and the dissolution efficiency and the yield of subsequent extraction are greatly improved. Subcritical water extraction has the outstanding advantages of high extraction efficiency, high speed, no need or great reduction of the use amount of organic solvents, environmental friendliness and the like.

The invention relates to the field of plant extracts, in particular to a torreya grandis total RNA extract which at least comprises mRNA, tRNA, miRNA and tsRNA. The torreya grandis total RNA extract can be used as a natural active matter for an external preparation for the skin, and the effects of resisting oxidation, resisting aging or resisting wrinkles and tightening are achieved, so that the skin aging is delayed.

The capsule may be exposed to an alternating magnetic field to release the active material, and may include a core containing the active material and a shell surrounding the core, at least one of the core and the shell may include at least one induction heating element particle heated by exposure to the alternating magnetic field.

The invention discloses a polysaccharide aldehyde modification method based on a plasma technology, relates to the technical field of biological material modification, and aims to solve the problems of environmental pollution, harsh reaction conditions and complex product purification in the conventional chemical oxidation method. Comprising the following steps: S1, preparing a polysaccharide solution; dissolving polysaccharide in a solvent, and stirring until the polysaccharide is completely dissolved to obtain a homogeneous polysaccharide solution; and S2, plasma in-situ oxidation treatment. In an inert gas atmosphere environment, plasma is generated by using a coaxial dielectric barrier discharge device, and active substances of the plasma directly act on a polysaccharide solution, so that an aldehyde group is introduced to a polysaccharide molecular chain, and an aldehyde polysaccharide solution is obtained. And S3, freeze-drying and forming. And freeze-drying the obtained aldehyde polysaccharide solution to obtain the aldehyde polysaccharide solid material with the three-dimensional porous network structure. The method is simple and convenient to operate, easy to popularize, simple in equipment, short in process, free of complex purification steps and beneficial to laboratory and large-scale production and application.

To provide a technique for delivering an active substance to the surface of a target cell.SOLUTION: According to one embodiment, a particle-bound lipid particle includes a biodegradable lipid particle, a particle, and active substances, wherein the particle is bound to an outer surface of the lipid particle, and the active substances are bound to a surface of the particle.SELECTED DRAWING: Figure 1

Methods of forming patterned features on a surface of a substrate are disclosed. Exemplary methods include gas-phase formation of a layer comprising an oxalate compound on a surface of the substrate. Portions of the layer comprising the oxalate compound can be exposed to radiation or active species that form exposed and unexposed portions. Material can be selectively deposed onto the exposed or the unexposed portions.

A method and system of adaptive cold atmospheric based treatment for diseased tissues, such as an area with cancerous cells, is disclosed. A gas such as helium is ionized via supplying power between and anode and a cathode to create an initial cold atmospheric plasma jet. The initial plasma jet is directed toward the area for a sufficient time, such as 10 seconds, to sensitize the cells via a reactive species. A reactive treatment, such as H2O2 or TMZ, is directed to the sensitized cells. The effectiveness of the reactive treatment is increased by the process of sensitizing the cells.

Presented are processes for the beneficial conversion of CO2 and other environmentally destructive compounds to their constituent parts by the application of thermal plasma containing activated species whereby the interaction of the plasma with the compounds and reactions of CO2 and H2 generate more environmentally friendly compounds comprising in part oxygen and hydrogen. The thermal plasma may be vibro-shear plasma generated by the superheating of either steam, gas or a combination of both.

The present invention belongs to the field of biomedicine technology, and discloses an engineered bionic exosome with anti-aging efficacy, a preparation method and application thereof, comprising the following steps: (S1) preparing a bionic cell membrane modified targeting ligand: adding collagen, mitochondrial targeting polypeptide, condensing agent, and 4-dimethylaminopyridine to an organic solvent, activating under a protective atmosphere, and then adding lysine to the above system for reaction to obtain a bionic cell membrane modified targeting ligand; (S2) embedding the bionic cell membrane modified targeting ligand into an engineered exosome bionic membrane, and encapsulating active substances to form an engineered bionic exosome. The exosome of the present invention has better skin permeability, mitochondrial targeting effect, and cell endocytosis effect, has a better protective effect on mitochondria, and can achieve efficient delivery of active substances.

The invention discloses a palladium-loaded magnetic silicon dioxide nanofluid, a preparation method, application and an oil-displacing agent, the nanofluid is obtained by adopting a green biological base solution, and surface active substances generated by microbial metabolism have a stable dispersion effect; the magnetic core can be recycled by virtue of the action of a magnetic field, the shell layer material provides stable dispersing capacity, and the loaded palladium can inhibit the blockage damage of microorganism breeding to the stratum; the bioactive components and the nanoparticles can synergistically improve the crude oil recovery rate, the oil recovery mechanism is disclosed through microscopic oil drop stripping and imbibition experiments, and the core displacement experiment effect is remarkable.

The present invention relates to the technical field of microbiological devices, and in particular to an integrated device and method for microalgae cultivation, harvesting and oil preparation. Wastewater to be treated and an appropriate amount of microalgae cells are sequentially added from an injection port into a microalgae cultivation main chamber, and then the temperature, light, and stirring speed parameters are set according to predefined optimal culture conditions to start microalgae cultivation and wastewater treatment. The present invention realizes efficient and clean treatment of various types of wastewater, especially seaweed processing wastewater, and rapid harvesting of microalgae cells. The obtained biomass can be further used for the extraction of high-value active substances such as bio-oil and the development of microalgae biological products, thereby generating considerable economic benefits. In addition, by integrating the treatment of wastewater, the harvesting of highly economical microalgae, and the preparation of active substances, rapid separation of microalgae cells from culture fluid and efficient preparation of oil are realized, thereby effectively simplifying the harvesting process of large-scale microalgae cultivation and significantly reducing the harvesting cost.

A method for filling a gap in a substrate is disclosed. The method includes providing a substrate with a gap in a reaction chamber, forming first active species from a first reactant to create an inhibition layer near the top of the gap, and performing one or more deposition cycles to deposit material into the gap. Each deposition cycle involves introducing a second reactant to form a chemisorbed layer and generating a second active species from a third reactant using pulsed plasma power, which reacts with the chemisorbed layer to form a deposited layer. The inhibition layer partially inhibits deposition at the top of the gap. The deposited material comprises silicon nitride, which is subsequently converted to silicon oxide by providing a fourth reactant containing hydrogen and oxygen. This conversion results in volume expansion and improved gap filling.

To provide compositions and methods for mediating EPS. [Solution] This disclosure relates to a method for inhibiting the stability of a biofilm, comprising contacting the biofilm with an effective amount of an active substance that interferes with the binding of polyamines to DNA in the biofilm. Also provided herein is a method for treating a biofilm in a subject, comprising administering an effective amount of an active substance that interferes with the binding of polyamines to DNA in the biofilm to a subject infected with a biofilm. Further described herein is a method for treating a biofilm in a patient with systemic lupus erythematosus (SLE) and / or cystic fibrosis (CF), comprising administering an effective amount of an active substance that interferes with the conversion of B-DNA to Z-DNA in the biofilm or its local environment.