Bacillus belleus GT11 and application thereof

A technology of Bacillus Velez and Bacillus, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of soil structure damage, failure to achieve disease control effects, etc., and achieve good comprehensive properties, efficient control effects, and good bacteriostasis effect of effect

Inactive Publication Date: 2017-12-08
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term application or excessive application of quicklime, in addition to causing damage to the soil structure, in many cases cannot achieve good disease control effects

Method used

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  • Bacillus belleus GT11 and application thereof
  • Bacillus belleus GT11 and application thereof
  • Bacillus belleus GT11 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of Bacillus velezensis GT11 Aqua

[0029] (1) Bacteria culture

[0030] a. test tube culture (below are weight percentages)

[0031] Put NA medium (5g of peptone, 10g of sucrose, 1g of yeast powder, 3g of beef extract, 17-20g of agar) into a test tube, make up to 1000mL with distilled water, sterilize, make a plate, and inoculate Bacillus velei GT11 Strains, cultivated at 28°C for 48h.

[0032] b. Bacteria Shaker Expansion Cultivation

[0033] The plate seeds were inoculated on the NA medium of the eggplant bottle slant (the medium formula is the same as the above-mentioned step a), and cultured at 26° C. for 48 hours to obtain the eggplant bottle seeds.

[0034] (2) fermentation

[0035] The specific fermentation production process is as follows:

[0036] a. Preparation of seed suspension: wash the colony on the slope of the eggplant bottle with sterilized water to obtain the seed suspension;

[0037]b. Inoculation: the seed suspension is passed throu...

Embodiment 2

[0042] Preparation of Wettable Powder of Bacillus velezensis GT11

[0043] (1) prepare liquid biocontrol agent: with embodiment 1, obtain fermented liquid;

[0044] (2) Add filler: Put the fermentation hydraulic pressure into the storage tank, add diatomite (light calcium carbonate) as filler according to the following calculation formula, and stir for 30 minutes;

[0045] Adding amount of filler (kg)=[number of fermentation broth bacteria (100 million / ml) X tank volume (liter) X yield] / number of finished product bacteria-residue in fermentation broth (kg). Then, it is filtered by plate and frame, beaten, dried, crushed, and made into wettable powder for field use.

[0046] (3) Plate and frame filtration: use 2kg / cm 2 Press the material into the plate frame from the storage tank, filter through No. 7 filter cloth, adjust the pressure at any time, so that the bacteria content in the filtrate is not more than 0.2 billion / ml, and the filter cake and filtrate are obtained;

[0...

Embodiment 3

[0052] In Vitro Antibacterial Test of Bacillus velezensis GT11 on Inchi Fruit Root Rot

[0053] The media used are as follows:

[0054] KB medium (peptone 20g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate heptahydrate 1.5g, glycerol 10mL, distilled water 1000mL; pH7.0);

[0055] PDA medium (potato 200g, agar 15g, glucose 15g, water 1000mL);

[0056] NA medium (peptone 5g, beef extract 3g, yeast extract 1g, sucrose 10g, agar 17g, water 1000mL, pH7.0);

[0057] 1. Bacillus velezensis (Bacillus velezensis) GT11 in vitro antibacterial test on Inki fruit root rot and other pathogenic bacteria

[0058] 1.1 Pathogens tested

[0059] Including Fusarium oxysporum, Fusarium solani, Botrytis cinerea, Cylindrocarpon destructans, Phytophthora parasitica var.nicotianae, etc., the above pathogens Provided by the Plant Pathology Laboratory of Plant Protection College, Yunnan Agricultural University.

[0060] 1.2 Test method

[0061] Activation of pathogenic bacteria: move the ...

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Abstract

The invention discloses a bacillus belleus GT11 and application thereof. The bacillus belleus CT11 is collected at China General Microbiological Culture Collection Center on December 13th, 2016; the collection number is CGMCC No.13446; the bacillus belleus CT11 can be prepared into water agent, wettable powder or coating material and can be used for preventing and controlling the root rot diseases of pseudo-ginseng and plukenetia volubilis and Chinese cabbage clubroot. The invention provides an efficient, nontoxic, safe, non-residual and conveniently used biological agent for preventing and controlling the diseases such as root rot and clubroot; the effective active ingredient is bacillus belleus CT11 strain; the strain can generate the secondary metabolite antimicrobial active matter, is capable of degrading the cell wall of pathogenic bacteria and has an excellent growth-promoting effect for the crops, such as, plukenetia volubilis, pseudo-ginseng and Chinese cabbage; besides, the bacillus belleus CT11 has a better antibacterial effect for pseudo-ginseng phytophthora sojae, pseudo-ginseng fusarium solani, plukenetia volubilis gray mold, paddy bacterial mycosphaerella, and the like.

Description

technical field [0001] The invention relates to the technical field of biocontrol agents, in particular to a strain of Bacillus Velez GT11 and its application. Background technique [0002] Sacha inchi (Plukenetia Volubilis., sacha inchi), also known as Sacha inchi, South American oil vine, and American vine fruit, is a woody vine and perennial oil crop belonging to the family Euphorbiaceae. Native to the Amazon River Basin in Peru, South America, this plant has been eaten in the Inca region of South America for thousands of years. Inchi fruit has many health functions such as regulating blood lipids, preventing cardiovascular diseases, and anti-aging. Inchi oil, the content of three kinds of unsaturated fatty acids (α-linolenic acid, linoleic acid and oleic acid) is more than 92%, and the content of α-linolenic acid (43.83%-48.6%) is significantly higher than that of olive oil and tea Seed oil and other edible oils; Sacha Inchi oil also contains lipid active substances (t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 姬广海魏兰芳朱书生付丽娜杨俊汪娅婷王星
Owner YUNNAN AGRICULTURAL UNIVERSITY
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