62 results about "South american" patented technology

The invention discloses a bacteriophage for preventing and treating a prawn vibrio parahaemolyticus disease and an expanding culture method thereof. Vibrio parahaemolyticus infected by South American white prawn are used for successfully screening out a strong lytic bacteriophage in seawater, titer of the bacteriophage is up to 1010 to 1012pfu/mL, fermentation period is 8 to 12h, treatment effects are obvious, and the bacteriophage for preventing and treating the prawn vibrio parahaemolyticus disease is of great significance for treatment of diseases causes by the vibrio parahaemolyticus and development of bacteriophage microecological preparations. A storage unit code of the bacteriophage is China Center for Type Culture Collection, address is China Center for Type Culture Collection in Wuhan University Campus, Wuchang Luojia Mountain, Wuhan City, preserved date is December 9, 2016, preservation number is CCTCC NO: M 2016740, a preservation test result is survival, name is vibrio parahaemolyticus bacteriophage VP11, and classification is named as Vibrio parahaemolyticus bacteriophage VP11.

Soybean rust occurs in many countries throughout Asia, Australia, Africa, and South America. The causal agents of soybean rust are two closely related fungi, Phakopsora pachyrhizi and P. meibomiae, which are differentiated based upon morphological characteristics of the telia. Determination of the nucleotide sequence of the internal transcribed spacer (ITS) region revealed greater than 99%/95% nucleotide sequence similarity among isolates of either P. pachyrhizi or P. meibomiae, but only 80% sequence similarity between the two species. Utilizing differences within the ITS region, four sets of PCR primers were designed specifically for P. pachyrhizi, and two sets of PCR primers were made specific to P. meibomiae. Classical and real-time fluorescent PCR assays were developed to identify and differentiate between P. pachyrhizi and P. meibomiae.

An ecologic reproduction method for the South-American white prawn features that a lighting method is used to culture parent sprawns. After the illuminance is increased, the water quality is improved and the algae can grow on the inner surface of culturing pool for meeting the requirement of parent prawns.

Disclosed is an environment-friendly type compound feed for South American prawn which comprises the following constituents (by weight ratio): red fish meal 15-25kg, soybean meal 10-20g, peanut bran 10-15kg, vegetable seed waste 3-7kg, meat and bones meal 5-10kg, squid internal organ powder 3-8kg, high grade flour 18-25kg, soybean lecithin 1.0-2.0kg, sea fish oil 2.0-3.0kg, choline chloride 0.3-0.6kg, vitamin C and phosphoric ester 0.05-0.1kg, immunopotentiator 0.1-0.2kg, calcium biphosphate 0.5-1.0kg, composite vitamins 0.2-0.5kg, and composite mineral salts 0.3-0.7kg.

The invention discloses a high-efficiency ecological breeding method for South American white shrimps. The high-efficiency ecological breeding method includes the steps such as breeding greenhouse building, offspring seed selection, stocking preparation, breeding management, water quality management. The technology is advanced and applicable; operability is strong; and breeding risk of South American white shrimps is reduced. Disease incidence is reduced. Dissolved oxygen of varied water layers in greenhouses is improved; condition of bottom layer water is improved; improvement in appetites of the South American white shrimps is benefited; and digestibility and utilization rate of feeds are increased.

The invention provides a method for efficiently breeding South American white shrimps. The method comprises the following steps that firstly, at the desalination stage, shrimp fry conveyed from Haian Province are conveyed to a mud pool to be desalinated for 7-8 days, the temperature is controlled between 17 DEG C and 35 DEG C, the aeration is carried out through aerators for 2.5-4.5 hours, at the moment, bittern is poured into the mud pool, and the salinity of the mud pool is desalinated by using fresh water every day, wherein the length of the white shrimps ranges from 5 centimeters to 6 centimeters; secondly, at the breeding stage, the shrimp fry are placed in a pond with the area ranging from 8 mu to 15 mu to be bred for 3 months, the stocking density of the shrimp fry ranges from 120 thousand fry per mu from 15 thousand dry per mu, the transparency of the pond ranges from 20 centimeters to 30 centimeters, the pH ranges from 7.9 to 8.3, the two or three aerators are used for aerating, and feeding fodder is added in the process of breeding and is mixed with vitamins and probiotics promoting substances. The method has the advantages that the rate of survival of the South America white shrimps and the nutritive value of the white shrimps are improved, the growth of an effective microbial community in a water body is promoted, the improvement of the water quality is promoted, and eutrophication caused by unnecessary fodder is avoided.

The invention relates to a special feed additive for a South American white shrimp. The special feed additive is prepared from the following raw materials by weight: 5 to 10 parts of perilla, 5 to 8 parts of Chinese honey locust, 5 to 7 parts of green tangerine peel, 2 to 4 parts of cassia twig, 2 to 3 parts of aniseed, 3 to 7 parts of white peony root, 0.02 part of vitamin C, 0.001 part of vitamin K and 20 to 25 parts of a carrier. 0.5 to 1.2% of the special feed additive for the South American white shrimp provided by the invention is added into a conventional mixed feed for the shrimp for breeding of the South American white shrimp. With the special feed additive for the South American white shrimp provided by the invention, economic benefits in breeding of the shrimp are obviously improved, occurrence of diseases during breeding of the shrimp is substantially reduced, the survival rate of the shrimp is increased by 19 to 22%, growth of the shrimp is promoted, and quality of the shrimp is enhanced.

The invention aims to provide an aquaculture process for South American eel larvae. The aquaculture process includes cleaning aquaculture ponds, and controlling the water temperatures and the salinity of the eel fry ponds. The aquaculture ponds are soaked or cleaned by the aid of dry quicklime powder, bleaching powder, potassium permanganate, clear water and the like; the water temperatures and the salinity of the aquaculture ponds are controlled in such modes that temperature difference of the ground are regulated, water is blended, salt is added into the water at specified steps, the water is diluted at specified steps and the like. The aquaculture process has the advantages that cleaning optimization schemes implemented before the aquaculture ponds are utilized are optimized by means of exploration, and methods for removing cement alkali are improved; aquaculture pond sterilization modes are optimized; the growth speeds of the eel larvae are increased, eel deformity can be prevented, and the death rates in larva aquaculture periods can be decreased; eel larva releasing modes and salinity regulation modes are modified, and accordingly death of the eel larvae due to temperature or salinity problems in releasing procedures further can be reduced.

The present invention provides isoformes from a peptide family belonging to South American scorpion Tityus serrulatus that acts as hypotensive agents by potentiating Bradykinin and, therefore, can be used as anti-hypertensive drugs. A peptide was firstly isolated from Tityus serrulatus venom and showed a strong and long-lasted hypotensive activity when tested in rats. This peptide was first named TsHpt-I (Tityus serrulatus Hypotensin-I). Also, three more highly similar isoformes were identified and revealed a peptide family with very close primary structure. They were named TsHpt-II, TsHpt-III and TsHpt-IV.

The invention belongs to the technical field of natural pharmaceutical chemistry, and relates to Borojo acid 1 to Borojo acid 13 separated from South American fruit, namely Borojo, and a preparation method and application of the Borojo acid 1 to Borojo acid 13. The preparation method comprises the steps that Borojo mature fruits serve as raw materials, and are peeled and subjected to seed removing, water is added into pulp, even mixing and grinding into thick liquid are conducted, or water is added into Borojo freeze-dried powder, even mixing and filtering are conducted, ethyl acetate is addedinto a filtrate for extraction, an ethyl acetate layer is concentrated to be dry and then subjected to chromatographic separation technologies such as silicone, octadecylsilane chemically bonded silica (ODS), sephadex LH-20 and preparative high performance liquid chromatography (Pre-HPLC), and thirteen Borojo acid compounds are obtained through separation from the Borojo, wherein the Borojo acid1 to the Borojo acid 10 are new compounds, and the other three Borojo acid compounds are known compounds obtained through first separation from the Borojo. Experiments show that the prepared compoundshave activities of resisting oxidation, resisting inflammation and reducing blood glucose.

The invention provides protein feed derived from rhodopseudomonas palustris and a preparation method and application of the protein feed. The protein feed is prepared from the following components inparts by weight: 90-93 parts of a broken wall thallus, 2-3 parts of lysine hydrochloride, 0.1-0.5 part of methionine, 0.1-0.5 part of threonine, 0.3-1 part of arginine, 2-3 parts of calcium hydrophosphate and 1-2 parts of calcium carbonate, wherein the broken wall thallus is the rhodopseudomonas palustris; and the preparation method includes the steps of thallus collection, lysozyme pretreatment,thallus wall breaking and formula combination, the wall breaking rate of a rhodopseudomonas palustris thallus reaches 100% by adopting the preparation method, the amino acid ratio is ideal, and the animal absorption use ratio is high. The invention further discloses application of the protein feed derived from the rhodopseudomonas palustris in duroc*(large white*landrace) ternary miscellaneous piglets, Qingyuan local pullets, carassius auratus gibelio and South American white shrimps. The protein feed is used for replacing corresponding fish meal, animal growth performance is better or not affected, and the protein feed is ideal.

The invention provides an indoor small water body cultivating method for South American white shrimps at temporary cultivation stage for thickening. The method comprises following steps: S1. disinfecting a container and water body, wherein the water carrying capacity of the container is 0.1 to 0.5 ton; S2. selecting healthy shrimp seeds; S3. temporary cultivating for thickening: the cultivating period is 20 to 30 days, water is not changed at the first eight days during cultivation, and EM bacterium is spread in the container every other day, after the first eight days, water is changed every day and EM bacterium is spread after water changing; shading treatment is carried out at the first eight days and cultivation under natural light is carried out after the first eight days; during the cultivation process, feeding for 12 times at schedule time is carried out daily and the intervals of 90 to 120 minutes are between each feeding; 3 to 5 days before the end of the temporary cultivating for thickening, vitamin C solution is spread daily for strengthened treatment for the shrimp seeds. The survival rate of shrimp seeds obtained by the temporary cultivating method for thickening is high and the growth is fast.

The invention relates to a Chinese herbal medicine micro-ecological preparation for resisting transport stress in Penaeus vannamei, which is prepared from the following raw materials in parts by weight: 22-26 parts of gallnut, 14-21 parts of poplar flower, 5-20 parts of gentian, Cen 10-15 parts, Wujiapi 5-10 parts, fermentation strains and liquid culture medium. The preparation is obtained by fermenting the raw materials of Chinese herbal medicine in a liquid culture medium through fermentation strains. The beneficial effects of the present invention are as follows: 1. The raw material components of the compound Chinese herbal medicine have wide sources, low cost, simple preparation, and significant effects; 2. The fermentation strain is a beneficial bacterial strain capable of decomposing and producing antioxidant substances, and can release a large amount of active ingredients of the Chinese herbal medicine , and can improve shrimp disease control and intestinal health to a certain extent; 3. It can significantly reduce the stress red body phenomenon that occurs during the transportation of prawns; 4. It has obvious economic value when applied to Penaeus vannamei. The survival rate of transportation has been significantly improved.