8 results about "Alpha Gene" patented technology

The invention discloses a group of chicken PGC-1alpha gene promoters as well as a construction method and application thereof, and belongs to the field of gene engineering. The chicken PGC-1 alpha gene promoter is any one of A1-A5 sequences of which the nucleotide sequences are shown as SEQ ID NO: 1 to SEQ ID NO: 5. The promoter is applied to the promotion of expression of firefly luciferase in chicken preadipocytes, and is applied to the regulation and control of the chicken PGC-1 alpha gene promoter by a specific transcription factor PPAR gamma. The five PGC-1alpha gene promoters have promoter activity in chicken preadipocytes, and can directly regulate and control transcription expression of the chicken PGC-1alpha gene. The chicken PGC-1alpha promoter disclosed by the invention can predict the transcriptional activity of the PGC-1alpha at the in-vitro cellular level, so that the chicken PGC-1alpha promoter has potential application values for revealing a transcriptional regulation mechanism of the chicken PGC-1alpha, breeding low-abdominal-fat broilers, revealing molecular mechanisms after various human diseases occur and developing drugs for targeting PGC-1alpha expression.

The invention relates to an application of a FaPDHE1alpha gene or an encoding protein thereof in prevention and control of strawberry gray mold, and belongs to the field of plant genetic engineering and the field of prevention and control of postharvest diseases of fruits and vegetables. The invention discloses a key positive regulation and control effect of FaPDHE1alpha in regulation and control of strawberry gray mold resistance. By constructing a 35S:: FaPDHE1alpha overexpression vector and a FaPDHE1alpha RNAi interference vector, according to transient gene overexpression and RNA interference silencing tests, it is verified that high expression of the FaPDHE1alpha gene can obviously enhance the resistance of strawberry fruits to botrytis cinerea, and silencing of the gene causes reduction of the disease resistance of the fruits. Mechanism studies show that after the FaPDHE1alpha gene is activated, mitochondrial energy metabolism and controlled ROS signal generation are promoted, antioxidant enzyme activity is enhanced, and defense related gene expression is induced, so that the disease resistance of fruits is improved. By means of functional genomics, the key function of the FaPDHE1alpha gene in prevention and control of gray mold after strawberry harvest is illuminated for the first time, and the FaPDHE1alpha gene can be used for screening disease-resistant breeding materials or developing biological prevention and control preparations for postharvest diseases and has important application value.

The invention belongs to the technical field of microbiological detection. The piriformospora indica PCR (Polymerase Chain Reaction) detection specific primer is characterized in that the nucleotide sequence of the specific primer is as follows: an upstream primer PI-F: 5 '-CGCTGGAGGAATTTGAGAAG-3'; the downstream primer PI-R is 5 '-CCGTCTTCCCGATAATGATG-3', and the downstream primer PI-R is 5 '- The invention is used for solving the technical problems of high false positive and low amplification efficiency caused by low specificity of the existing EF1 alpha gene detection marker. The invention also discloses a fluorescent quantitative PCR detection method for piriformospora indica, which is realized by using the primer.

The present application relates to a kind of pig alpha interferon mutant and its preparation method and application, the mutant amino acid sequence is as shown in SEQ ID NO.2.Preparation, with original PIFN-alpha gene as template, according to CHO-K1 cell codon bias optimization gene sequence, again with murine IGk signal peptide replaces traditional CHO signal peptide, while the glutamine of N-terminal 155th is replaced by the alanine with anti-degradation characteristics, by introducing N-terminal regular anti-degradation site to extend protein half-life, finally, the nucleotide sequence encoding the mutant is obtained by directional optimization.It is second, the present application is by constructing the recombinant expression vector pCHO1.0-PIFN-Bip containing double promoter synergistic expression system, and it is transferred into CHO cell and is expressed, successfully realize the efficient expression of pig alpha interferon in CHO cell, and it also has significant effect to be applied to the treatment of pig viral infectious disease.

The invention relates to the field of genomes, in particular to an LTalpha humanized genome, a vector and application. According to the application, the human LT alpha gene is used for replacing the LT alpha gene of the mouse, so that the humanized LT alpha mouse can better simulate human immune response.

The invention discloses a method for improving wheat haploid inductivity, which is used for obtaining a wheat plant with haploid induction by reducing or silencing expression of wheat TaPLA and TaCENH3alpha genes in wheat. The TaPLA gene and the TaCENH3 alpha gene in the genome level can be silenced through a gene transformation process, and the TaPLA gene and the TaCENH3 alpha gene silencing method is a powerful tool for silencing the TaPLA gene and the TaCENH3 alpha gene by utilizing a gene engineering technology. The haploid inductivity of wheat can be improved by silencing the two genes at the same time.

The present disclosure relates to RNAi agents, for example, double stranded RNAi agents, able to inhibit HIF-2 alpha (EPAS1) gene expression. Also disclosed are pharmaceutical compositions that include HIF-2 alpha RNAi agents and methods of use thereof. The HIF-2 alpha RNAi agents disclosed herein may be linked or conjugated to targeting ligands (such as compounds that have affinity for integrins, including alpha-v-beta-3 and alpha-v-beta-5 integrins) and pharmacokinetic (PK) enhancers, to facilitate the delivery to cells and tissues, including to clear cell renal cell carcinoma (ccRCC) cells and tumors. Delivery of compositions comprising the HIF-2 alpha RNAi agents in vivo provides for inhibition of HIF-2 alpha gene expression. The HIF-2 alpha RNAi agents can be used in methods of treatment of various diseases and disorders, including ccRCC.

The invention provides a promoter and a screening method and application thereof. The promoter is obtained through a screening method based on a lacZ gene. Specifically, the method comprises the following steps: respectively carrying out library establishment and preliminary screening on plasmids which contain beta-galactosidase genes (lacZ or lacZ alpha genes) and have inactivated promoters, and then carrying out secondary screening on the basis of a chromogenic reaction and enzyme activity determination between a chromogenic substrate and beta-galactosidase, so as to obtain the promoter with required strength. According to the technical scheme, the screening process is simplified, the screening efficiency is improved, and a new thought and a new tool are provided for research and application of the promoter.