The invention discloses a
molecular marker and application of a
rapeseed recessive nuclear sterile
gene segment. The steps are as follows: A. Positioning BnMs3 and Bnrf in the
Brassica oleracea by controlling the location of the
rapeseed recessive nuclear sterile
gene BnMs3 and Bnrf A genetic interval of less than 0.2cM on the N19 and N7 linkage groups of
rapeseed; B. The TapidorBAC
library was screened by marker screening combined with Southern hybridization, and the target clones JBnB043L23 and JBnB089D05 containing BnMS3 and Bnrf were obtained; C. BnMs3 was obtained by the sequencing of the target BAC The
nucleotide sequence of the
candidate gene segment with Bnrf; D, the difference between the
nucleotide sequence of the BnMs3 and the allelic recessive
gene Bnms3 gene segment according to C step and the difference between BnRf and the allelic recessive gene Bnrf gene segment Differences between
nucleotide sequences, development and design of primers, and development of molecular markers for the BnMs3 and BnRf sterile gene segments. The application of molecular markers in the
molecular marker-assisted breeding of the recessive genetic male sterile line and the temporary protection line of
Brassica napus makes the
molecular marker-assisted transfer of the recessive genetic male sterile line and the temporary protection line of rapeseed faster, more accurate and more accurate. efficient.