52 results about "Nuclear gene" patented technology

The invention discloses a screening method of a gene region set for detecting small residual focuses of ovarian cancer, the gene region set and a detection system of the gene region set, and belongs to the technical field of gene molecular diagnosis. According to the method, a preoperative blood sample, an intraoperative multipoint tumor tissue sample and postoperative daily peritoneal drainage liquid of an ovarian cancer patient on the first to sixth days are taken as detection samples, a novel ovarian cancer nuclear genome hot spot region screening method is developed, a gene region for ovarian cancer tumor DNA detection is constructed, and tumor tissue mutation information is taken as a tracing basis, so that an ovarian cancer tumor DNA detection result is obtained. The characteristics of tumor tissue specific mutation in the free DNA of the peritoneal drainage liquid are analyzed, and the purpose of detecting the free tumor DNA of the peritoneal drainage liquid is achieved. The method can be applied to the postoperative residual focus detection of the ovarian cancer patient, can sensitively and accurately evaluate the postoperative residual focus state of the patient at an early stage, and provides timely prognosis judgment basis for clinic, so as to support the adjustment of clinical decisions.

The invention discloses a specific primer for distinguishing rice and barnyard grass and application of the specific primer, and belongs to the technical field of biological species identification. The sequences of the specific primers are as shown in SEQ ID NO. 1 and SEQ ID NO. 2. The specific primer is designed according to the nuclear gene ITS sequence of the rice, the specific primer can accurately identify the rice through PCR, the identification method is stable, the detection time is short, the specificity is high, the detection limit is low, the identification method is not affected by material characters, rapid and accurate identification of the rice and barnyard grass can be achieved, and the identification efficiency is high. The problem that a method for effectively and rapidly distinguishing rice and barnyard grass does not exist at present is solved.

The application discloses a method, device and storage medium for detecting mitochondrial-derived nuclear genome sequences. The method comprises the following steps: aligning whole genome sequencing data to a mitochondrial reference genome rCRS, retaining the reads aligned to the rCRS, aligning the reads to a reference sequence containing 23 pairs of chromosomes and the rCRS, removing non-unique alignment reads; extracting potential junction reads, clustering the junction reads within a distance of 50 bp according to the alignment positions, locating the coordinates and directions of the integrated mitochondrial DNA fragments, and the nuclear genome integration position; searching for inconsistent alignment read pairs within 100 bp upstream and downstream of a read cluster, and counting the number of the inconsistent alignment read pairs as support information for the existence of non-ref NUMTs. The application utilizes the alignment information of the junction reads, detects the non-ref NUMTs through local assembly and clustering, reduces the false positive rate, can obtain accurate breakpoint and fragment information, is simple to operate, and has low detection cost.

Disclosed are: a cauliflower plant which is suitable for a cauliflower processed product divided into small florets and which enables the production of high-yield small florets accompanied by length of a stem part; and a processed product of the cauliflower plant. The cauliflower plant according to the present invention has in the nuclear genome thereof a gene involved in small floret production that enables the production of high-yield small florets accompanied by length of a stem part. The cauliflower plant according to the present invention is easily processed into small florets, making it possible to provide a cauliflower product divided into small florets having a high commercial value.

The invention discloses an HRM primer and application of the HRM primer to identification of plant-derived components of tara and gleditsia. Aiming at the defect of lack of a product and a method for identifying high-value gleditsia sinensis lam pretended by low-cost tara seed endosperm at present, nuclear gene DNA of tara, gleditsia sinensis lam for producing gleditsia sinensis lam and common congeneric related species thereof are analyzed; an HRM primer capable of distinguishing plant-derived components of tara and gleditsia is obtained based on ITS sequence design. On the basis of the HRM primer, the invention also provides a kit and a method for identifying the tara and the gleditsia plant-derived components, and the tara can be rapidly and specifically detected, so that the identification of the gleditsia sinensis seeds adulterated with the tara seed endosperm is realized. The method is beneficial to inspection of adulterated gleditsia sinensis fruits, and economic benefits of consumers and gleditsia sinensis fruit commercial tenants and enterprises are protected.

The invention discloses a ganoderma lucidum binuclear genome assembly method based on haplotype analysis, and belongs to the technical field of bioinformatics. The purpose of the present invention is to overcome the accuracy and accuracy of high hybrid genome assembly. The invention provides a ganoderma lucidum binuclear genome assembly method based on haplotype analysis, which comprises the following steps: obtaining mononuclear ganoderma lucidum cells, respectively carrying out whole genome sequencing by utilizing Illumina NovaSeq, Nanopore, Hi-C and PacBio HiFi, and obtaining accurate genome information according to the sequencing data of the Illumina NovaSeq and the sequencing data of the Nanopore. And an assembly strategy of haplotype separation is realized.

The application provides a fat sea base gene and adulteration identification method based on multi-gene joint DNA barcoding, four gene joint barcode combinations are formed by adopting nuclear gene ITS2 and chloroplast genes matK, rbcL and psbA-trnH, at least two pairs of optimized alternative primers for each target gene and fat sea genus specific preliminary screening primer Ster-1 are matched, genus level rapid preliminary screening is realized to exclude non-fat sea genus samples and shorten the identification process, and the efficient amplification success rate of multi-gene fragments is ensured through the flexible use of alternative primers; fat sea and round fat sea, Sterculia and mixed adulterants can be accurately distinguished, the problems that traditional identification methods are subjective and species with close genetic relationship are difficult to distinguish are effectively solved; the method is not limited by sample morphology and fragmentation degree, can be widely applied to the true and false identification, quality control and traceability of traditional Chinese medicinal materials fat sea and its products, and provides technical support for standardizing market order and ensuring the safety and effectiveness of clinical medication.

The present invention provides a method for assembling the mitochondrial genome of American ginseng, which solves the technical problem that the existing TIPPo method has a high dependence on sequencing depth and requires a high mitochondrial genome coverage depth, otherwise it is difficult to accurately distinguish mitochondrial and nuclear genome fragments. The method can be widely used in the field of plant gene assembly. Specifically, it includes the following steps: inputting initial mitochondrial sequencing data, including second-generation sequencing and third-generation sequencing; assembling and extracting mitochondrial contigs using the second generation, and screening candidate mitochondrial contigs; aligning the third-generation data to the candidate mitochondrial contigs, extracting mitochondrial reads, and screening mitochondrial long reads; assembling the mitochondrial long reads to generate a mitochondrial assembly result; simplifying the mitochondrial assembly result to obtain a graphical mitochondrial genome, correcting the graphical mitochondrial genome, and generating accurate mitochondrial genome data.

The invention discloses a DNA bar code system and method for identifying glycyrrhiza medicinal plants and hybrid complexes thereof, and belongs to the technical field of molecular biology and traditional Chinese medicine identification. The system is formed by combining three bar codes of a nuclear gene segment ITS, a chloroplast gene segment ndhA and a chloroplast gene spacer trnH-psbA. Wherein ndhA is a core bar code which is screened and verified for the first time, and the specific variation site of the ndhA can be used for accurately distinguishing three kinds of medicinal liquorice, namely, Glycyrrhiza uralensis Fischhex DC., Glycyrrhiza glabra. And Glycyrrhiza inflata Batalin. The system fully utilizes the characteristics of chloroplast paternal heredity of glycyrrhiza, not only can identify homozygous species, but also can efficiently analyze complex hybridization and introgression types, and traces the source of a male parent. Compared with a traditional multi-bar code combination, the method has the advantages of being high in identification success rate (up to 98.08%), easy and convenient to operate and low in cost, and is suitable for liquorice germplasm resource identification, medicinal material market rapid screening and improved variety breeding.

Bottleneck Sequencing System (BotSeqS) is a next-generation sequencing method that simultaneously quantifies rare somatic point mutations across the mitochondrial and nuclear genomes. BotSeqS combines molecular barcoding with a simple dilution step immediately prior to library amplification. BotSeqS can be used to show age and tissue-dependent accumulations of rare mutations and demonstrate that somatic mutational burden in normal tissues can vary by several orders of magnitude, depending on biologic and environmental factors. BotSeqS has been used to show major differences between the mutational patterns of the mitochondrial and nuclear genomes in normal tissues. Lastly, BotSeqS has shown that the mutation spectra of normal tissues were different from each other, but similar to those of the cancers that arose in them.

The invention discloses a quadruple real-time fluorescent PCR primer probe combination for identifying yak milk, yellow milk, genuine dzo milk and false dzo milk, a product and an identification method, and relates to the technical field of dairy product identification. The kit comprises a first primer probe group for detecting yak nuclear genes, a second primer probe group for detecting yak mitochondrial genes, a third primer probe group for detecting cattle nuclear genes and a fourth primer probe group for detecting cattle mitochondrial genes. According to the primer probe combination and the identification method provided by the invention, yak milk, yellow milk, dzo milk and false dzo milk can be accurately identified; meanwhile, the method can accurately identify dzo milk or cow milk adulteration as low as 1% in the yak milk, and can meet the requirements of purchased samples and commercially available samples for dzo milk adulteration identification in the yak milk.

The present application discloses a kind of Magnaporthe grisea and Xanthomonas oryzae resistance related protein derived from rice and its coding gene and application.The present application provides a kind of method for improving the disease resistance of plant: the gene of plant coding OsAspRS protein is replaced by the gene of mutant protein.The present application also protects the application of osasprs mutant in the preparation of plant with improved disease resistance.osasprs mutant is a kind of spot-like mutant plant controlled by single recessive nuclear gene, which is screened by EMS on Huanghuazhan plant.Furthermore, the present application successfully locates and clones AspRS gene, and the mutation of the gene site can cause spot-like phenotype on rice leaf, and further improve the broad-spectrum disease resistance of rice.The present application is helpful to improve the disease resistance of crop, provides gene resources and technical support for cultivating new rice varieties with broad-spectrum resistance, and has important significance and application value for crop disease resistance breeding work.

The invention belongs to the technical field of genes, and particularly relates to a molecular marker composition for identifying seedless characters of grapes and application of the molecular marker composition, a seedless gene VvAGL11 is taken as an entry point, and combined molecular markers are developed for a specific insertion segment INS of a newly developed VvAGL11 gene promoter region, a key mutation segment CDS of a coding region and a seedless molecular marker P3VvAGL11. According to the molecular marker composition, the identification efficiency of seedless characters is remarkably improved, the accurate application range of the molecular marker is refined, a more accurate and efficient identification means is provided for seedless breeding of grapes, and the breeding efficiency of the grapes is improved.

The invention provides a sheep-derived component rapid quantitative detection method based on dual real-time fluorescent PCR. According to the method, a single-copy nuclear gene GAPDH is taken as a target spot, a sheep specific primer and a probe are designed in a hypervariable region of the single-copy nuclear gene GAPDH, and an internal reference primer and a probe capable of identifying various common animal-derived components are designed in a conserved region of the single-copy nuclear gene GAPDH. As the target and the internal reference are located in the same gene and coexist in the same cell according to the proportion of 1: 1, quantitative deviation caused by gene copy number difference or expression fluctuation in a traditional two-step method is remarkably reduced. The detection strategy constructed by the invention has double advantages of specific recognition and broad-spectrum reference, is clear in quantitative basis and simple and convenient to operate, is suitable for rapid recognition and quantitative adulteration analysis of sheep-derived components in meat products, and has wide application prospects and supervision values.

The present disclosure relates to transgenic animals with nuclear genomes comprising knockouts of both alleles of an SLA-DQ gene and / or an SLA-DR gene. The disclosure provides transgenic pigs, transplant products from the transgenic pigs and xenotransplantation methods utilizing the transplant products for treatment of transplant rejection, especially in humans.

The current invention provides a synthetic prokaryotic genome comprising 5 or fewer occurrences of one or more sense codons; and / or a synthetic prokaryotic genome derived from a parent genome, wherein the synthetic prokaryotic genome comprises less than 10%, 5%, 2%, 1%, 0.5%, 0.1% of the occurrences of one or more sense codons, relative to the parent genome; and / or a synthetic prokaryotic genome comprising 100 or more, 200 or more, or 1000 or more genes with no occurrences of one or more sense codons.

The invention belongs to the technical field of gene engineering, and particularly relates to a dunaliella salina nuclear-cytoplasm dual expression system as well as an establishment method and application thereof. The system comprises a donor expression cassette and a ribonucleoprotein complex, the donor expression cassette and the ribonucleoprotein complex are jointly or successively introduced into dunaliella salina cells for gene knockout, or fixed-point insertion of exogenous genes is performed by using a homologous recombination or non-homologous end connection method; the donor expression box comprises a cell nucleus donor expression box and a chloroplast donor expression box; the chloroplast donor expression cassette comprises a homologous arm derived from a dunaliella salina chloroplast genome; the nuclear donor expression cassette comprises a homologous arm derived from a dunaliella salina nuclear genome. The system provided by the invention can be used for improving dunaliella salina genome level characters, and a recombinant algal strain obtained by utilizing the system can be used as a medicine for preventing and treating diseases.

The present disclosure relates to transgenic animals with nuclear genomes comprising knockouts of both alleles of an SLA-DQ gene and / or an SLA-DR gene. The disclosure provides transgenic pigs, transplant products from the transgenic pigs and xenotransplantation methods utilizing the transplant products for treatment of transplant rejection, especially in humans.

Bottleneck Sequencing System (BotSeqS) is a next-generation sequencing method that simultaneously quantifies rare somatic point mutations across the mitochondrial and nuclear genomes. BotSeqS combines molecular barcoding with a simple dilution step immediately prior to library amplification. BotSeqS can be used to show age and tissue-dependent accumulations of rare mutations and demonstrate that somatic mutational burden in normal tissues can vary by several orders of magnitude, depending on biologic and environmental factors. BotSeqS has been used to show major differences between the mutational patterns of the mitochondrial and nuclear genomes in normal tissues. Lastly, BotSeqS has shown that the mutation spectra of normal tissues were different from each other, but similar to those of the cancers that arose in them.

The application discloses a method for identifying Ectropis oblique and Ectropis grisescens, and belongs to the technical field of molecular biology. The application takes Ectropis oblique and Ectropis grisescens as research objects, systematically screens diagnostic different sites in the whole genome based on population genome resequencing data, and develops a nucleic gene molecular marker which can be used for rapid and accurate identification of close species. COI Compared with the mitochondrial markers in the prior art, the method has wider applicability and higher genetic variation sensitivity at the species and population levels. The molecular identification method established in the application provides reliable technical support for accurate identification of close pests and application of the close pests in plant quarantine and monitoring.

The present application belongs to the technical field of biological information detection, and discloses a nuclear genome copy number variation detection method, which can identify whether the sample to be detected is a female sample. If yes, the half value of the sequencing depth value of the X chromosome site and the sequencing depth value of the autosomal site are compared with the respective confidence interval to determine the copy abnormal site, the copy number value of the copy abnormal site is calculated for spatial clustering classification, and the normal copy class and the copy variation class are obtained. The copy abnormal site belonging to the copy variation class is determined as the copy variation site. Then, the copy variation sites with adjacent positions and the same variation type are combined to obtain CNV fragments. Therefore, the present application can automatically and accurately perform CNV detection of all nuclear genome chromosomes, improve the accuracy of sex chromosome CNV detection, and improve the resolution of RD sites while ensuring the stability, accuracy and sensitivity of the CNV fragments.

The present invention discloses a method for screening nuclear genes that are retroregulated by mitochondrial sterility genes responsive to rapeseed cytoplasmic male sterility. The method includes: cloning the mitochondrial sterility gene of rapeseed cytoplasmic male sterility pollen abortion; constructing a plant expression vector of the mitochondrial sterility gene and genetically transforming Arabidopsis thaliana; obtaining differentially expressed genes (DEGs); calculating the co-expression relationship between genes to obtain the expression regulation relationship and regulation direction between genes, thereby constructing a co-expression network diagram of genes; selecting candidate nuclear genes to construct double mutant homozygous Arabidopsis thaliana mutants; and identifying nuclear genes that can restore the pollen fertility of the mutants. The present invention solves the problem that existing methods cannot accurately identify nuclear genes involved in the nuclear-cytoplasmic interaction of mitochondrial sterility genes. By constructing double mutant mutants and observing pollen fertility, the present invention can efficiently find nuclear genes that are retroregulated by mitochondrial genes jointly regulated by mitochondrial sterility gene expression and involved in pollen abortion.

The invention discloses application of a nuclear gene ITS sequence in identification and / or distinguishing of pokeberry root and counterfeit products thereof. The 1881st site of the nuclear gene ITS sequence with the GenBank login number of OL824854.1 is T or A, and the 1996 site nucleotide is T or C; or the nucleotide at the 1938th site is G or A, and the nucleotide at the 2031st site is T or A; the radix phytolaccae is Phytolacca aericana L. L., and the fake product is Phytolacca latinia (Moq.) Maxim., the radix phytolaccae is Phytolacca aericana L. L., and the fake product is Phytolacca latinia (Moq.) Maxim. According to the invention, a section of ITS bar code sequence capable of simultaneously identifying Phytolacca latbenia (Moq.) Maxim. In a sample to be detected is found for the first time, and the detection method designed based on the section of sequence is simple and reliable, is not influenced by the characters of medicinal materials, and can be used for accurately identifying medicinal material powder; the method has the advantages of high objectivity, no dependence on subjective judgment and the like.

The present invention provides a Coptidis species identification primer pair and a Coptidis species identification method, belonging to the field of molecular identification technology. The Coptidis species identification primer pair and the Coptidis species identification method provided by the present invention can overcome the problem of poor Coptidis species identification effect in the prior art. The identification region targeted by the Coptidis species identification primer pair provided by the present invention is a chloroplast fragment, not a nuclear gene fragment, and there is no multi-copy situation. At the same time, it can avoid the common microbial contamination problem of traditional Chinese medicine materials. The identification region fragment is short, with a length of only 400bp, which is much shorter than other DNA barcode fragments. Since degradation is a common phenomenon in traditional Chinese medicine materials, shorter barcode fragments are beneficial to improving the PCR amplification efficiency of degraded materials. It has strong practical application significance in Coptidis species identification.

The application discloses a Ganoderma lucidum double-nucleus genome assembly method based on haplotype analysis and belongs to the technical field of bioinformatics. The application aims to overcome the precision and accuracy of high-hybrid genome assembly. The application provides a Ganoderma lucidum double-nucleus genome assembly method based on haplotype analysis, obtains single-nucleus Ganoderma lucidum cells, respectively performs whole genome sequencing on the cells by using Illumina NovaSeq, Nanopore, Hi-C and PacBio HiFi, obtains precise genome information from Illumina NovaSeq sequencing data and Nanopore sequencing data, and realizes a haplotype separation assembly strategy.

Provided herein are methods for conferring cytoplasmic male sterility (CMS) on a plant line. The methods include obtaining a first plant comprising a CMS cytoplasm that is also a haploid inducer (CHIP) and crossing it with a second plant that comprises a desired nuclear genome (DIP). The CHIP also comprises a cenh3 mutation and may contain an anthocyanin marker and a restorer factor. The method further comprises generating progeny from said cross. The progeny produced from the cross of the method is haploid and comprises the CMS cytoplasm of the CHIP as well as the desired nuclear genome of the DIP. The progeny further lacks any anthocyanin marker or restorer factor.

Provided herein are methods and compositions for increasing production of a target protein or functional RNA by a cell.

The invention provides a construction method of an expression vector of eukaryotic genes in prokaryotes, and belongs to the field of molecular biology. The method comprises the following steps: (a) designing a PCR (Polymerase Chain Reaction) primer pair for amplifying each exon in a specific transcription spliceosome cds sequence; (b) respectively amplifying each exon by using the PCR primer pair designed in the step (a) to obtain a PCR fragment of each exon; (c) mixing the n exon fragments obtained in the step (b) as a template, and carrying out overlapping PCR by using the forward primer P1-F of the first exon and the reverse primer Pn-R of the final exon to obtain a complete cds fragment of the specific transcription spliceosome; and (d) purifying and recovering the cds fragment of the specific transcription spliceosome obtained in the step (c), and connecting the cds fragment to a prokaryotic expression vector. Therefore, the problem that the cds fragment of the target transcription spliceosome cannot be obtained due to unknown temporal-spatial expression information of a luxury gene or a specific transcript is solved.

This invention provides information on the potato StTuA and StTuB genes and their applications in improving late blight resistance, abiotic stress resistance, and yield. This invention discovers and confirms that two chloroplast elongation factors, StTuA and StTuB, encoded by potato nuclear genes and synthesized in the cytoplasm, can promote leaf photosynthesis, significantly increase potato tuber yield, and significantly enhance resistance to late blight and abiotic stress. Therefore, this study provides two key target genes for improving potato yield and resistance; increasing the expression levels of StTuA and StTuB genes can significantly improve potato yield and late blight resistance.

The present invention provides a method for preparing allodiploid scallops, which is to screen for all-female individuals among the hybrid offspring of Argopecten irradians (♀) and Argopecten purpuratus (♂), fertilize the eggs of the all-female individuals with the sperm of Argopecten irradians, and cultivate the fertilized eggs to obtain allodiploid scallops; the screening is to screen for all-female individuals with a ratio of the nuclear genome derived from Argopecten irradians to the nuclear genome derived from Argopecten purpuratus in the genome higher than 1.5. The method of the present invention is different from the currently mainstream methods of inhibiting the release of polar bodies of diploid fertilized eggs by chemical reagents, salinity adjustment, cold and heat shock, etc., and the method of obtaining triploid offspring by crossing tetraploid and diploid parent scallops, and has opened up a brand-new allodiploid scallop breeding technology.