38 results about "Nuclear gene" patented technology

The application discloses a method, device and storage medium for detecting mitochondrial-derived nuclear genome sequences. The method comprises the following steps: aligning whole genome sequencing data to a mitochondrial reference genome rCRS, retaining the reads aligned to the rCRS, aligning the reads to a reference sequence containing 23 pairs of chromosomes and the rCRS, removing non-unique alignment reads; extracting potential junction reads, clustering the junction reads within a distance of 50 bp according to the alignment positions, locating the coordinates and directions of the integrated mitochondrial DNA fragments, and the nuclear genome integration position; searching for inconsistent alignment read pairs within 100 bp upstream and downstream of a read cluster, and counting the number of the inconsistent alignment read pairs as support information for the existence of non-ref NUMTs. The application utilizes the alignment information of the junction reads, detects the non-ref NUMTs through local assembly and clustering, reduces the false positive rate, can obtain accurate breakpoint and fragment information, is simple to operate, and has low detection cost.

Disclosed are: a cauliflower plant which is suitable for a cauliflower processed product divided into small florets and which enables the production of high-yield small florets accompanied by length of a stem part; and a processed product of the cauliflower plant. The cauliflower plant according to the present invention has in the nuclear genome thereof a gene involved in small floret production that enables the production of high-yield small florets accompanied by length of a stem part. The cauliflower plant according to the present invention is easily processed into small florets, making it possible to provide a cauliflower product divided into small florets having a high commercial value.

The invention discloses a ganoderma lucidum binuclear genome assembly method based on haplotype analysis, and belongs to the technical field of bioinformatics. The purpose of the present invention is to overcome the accuracy and accuracy of high hybrid genome assembly. The invention provides a ganoderma lucidum binuclear genome assembly method based on haplotype analysis, which comprises the following steps: obtaining mononuclear ganoderma lucidum cells, respectively carrying out whole genome sequencing by utilizing Illumina NovaSeq, Nanopore, Hi-C and PacBio HiFi, and obtaining accurate genome information according to the sequencing data of the Illumina NovaSeq and the sequencing data of the Nanopore. And an assembly strategy of haplotype separation is realized.

The application provides a fat sea base gene and adulteration identification method based on multi-gene joint DNA barcoding, four gene joint barcode combinations are formed by adopting nuclear gene ITS2 and chloroplast genes matK, rbcL and psbA-trnH, at least two pairs of optimized alternative primers for each target gene and fat sea genus specific preliminary screening primer Ster-1 are matched, genus level rapid preliminary screening is realized to exclude non-fat sea genus samples and shorten the identification process, and the efficient amplification success rate of multi-gene fragments is ensured through the flexible use of alternative primers; fat sea and round fat sea, Sterculia and mixed adulterants can be accurately distinguished, the problems that traditional identification methods are subjective and species with close genetic relationship are difficult to distinguish are effectively solved; the method is not limited by sample morphology and fragmentation degree, can be widely applied to the true and false identification, quality control and traceability of traditional Chinese medicinal materials fat sea and its products, and provides technical support for standardizing market order and ensuring the safety and effectiveness of clinical medication.

The present invention provides a method for assembling the mitochondrial genome of American ginseng, which solves the technical problem that the existing TIPPo method has a high dependence on sequencing depth and requires a high mitochondrial genome coverage depth, otherwise it is difficult to accurately distinguish mitochondrial and nuclear genome fragments. The method can be widely used in the field of plant gene assembly. Specifically, it includes the following steps: inputting initial mitochondrial sequencing data, including second-generation sequencing and third-generation sequencing; assembling and extracting mitochondrial contigs using the second generation, and screening candidate mitochondrial contigs; aligning the third-generation data to the candidate mitochondrial contigs, extracting mitochondrial reads, and screening mitochondrial long reads; assembling the mitochondrial long reads to generate a mitochondrial assembly result; simplifying the mitochondrial assembly result to obtain a graphical mitochondrial genome, correcting the graphical mitochondrial genome, and generating accurate mitochondrial genome data.

The invention discloses a DNA bar code system and method for identifying glycyrrhiza medicinal plants and hybrid complexes thereof, and belongs to the technical field of molecular biology and traditional Chinese medicine identification. The system is formed by combining three bar codes of a nuclear gene segment ITS, a chloroplast gene segment ndhA and a chloroplast gene spacer trnH-psbA. Wherein ndhA is a core bar code which is screened and verified for the first time, and the specific variation site of the ndhA can be used for accurately distinguishing three kinds of medicinal liquorice, namely, Glycyrrhiza uralensis Fischhex DC., Glycyrrhiza glabra. And Glycyrrhiza inflata Batalin. The system fully utilizes the characteristics of chloroplast paternal heredity of glycyrrhiza, not only can identify homozygous species, but also can efficiently analyze complex hybridization and introgression types, and traces the source of a male parent. Compared with a traditional multi-bar code combination, the method has the advantages of being high in identification success rate (up to 98.08%), easy and convenient to operate and low in cost, and is suitable for liquorice germplasm resource identification, medicinal material market rapid screening and improved variety breeding.

The invention discloses a quadruple real-time fluorescent PCR primer probe combination for identifying yak milk, yellow milk, genuine dzo milk and false dzo milk, a product and an identification method, and relates to the technical field of dairy product identification. The kit comprises a first primer probe group for detecting yak nuclear genes, a second primer probe group for detecting yak mitochondrial genes, a third primer probe group for detecting cattle nuclear genes and a fourth primer probe group for detecting cattle mitochondrial genes. According to the primer probe combination and the identification method provided by the invention, yak milk, yellow milk, dzo milk and false dzo milk can be accurately identified; meanwhile, the method can accurately identify dzo milk or cow milk adulteration as low as 1% in the yak milk, and can meet the requirements of purchased samples and commercially available samples for dzo milk adulteration identification in the yak milk.

The present application discloses a kind of Magnaporthe grisea and Xanthomonas oryzae resistance related protein derived from rice and its coding gene and application.The present application provides a kind of method for improving the disease resistance of plant: the gene of plant coding OsAspRS protein is replaced by the gene of mutant protein.The present application also protects the application of osasprs mutant in the preparation of plant with improved disease resistance.osasprs mutant is a kind of spot-like mutant plant controlled by single recessive nuclear gene, which is screened by EMS on Huanghuazhan plant.Furthermore, the present application successfully locates and clones AspRS gene, and the mutation of the gene site can cause spot-like phenotype on rice leaf, and further improve the broad-spectrum disease resistance of rice.The present application is helpful to improve the disease resistance of crop, provides gene resources and technical support for cultivating new rice varieties with broad-spectrum resistance, and has important significance and application value for crop disease resistance breeding work.

The invention belongs to the technical field of genes, and particularly relates to a molecular marker composition for identifying seedless characters of grapes and application of the molecular marker composition, a seedless gene VvAGL11 is taken as an entry point, and combined molecular markers are developed for a specific insertion segment INS of a newly developed VvAGL11 gene promoter region, a key mutation segment CDS of a coding region and a seedless molecular marker P3VvAGL11. According to the molecular marker composition, the identification efficiency of seedless characters is remarkably improved, the accurate application range of the molecular marker is refined, a more accurate and efficient identification means is provided for seedless breeding of grapes, and the breeding efficiency of the grapes is improved.

The invention provides a sheep-derived component rapid quantitative detection method based on dual real-time fluorescent PCR. According to the method, a single-copy nuclear gene GAPDH is taken as a target spot, a sheep specific primer and a probe are designed in a hypervariable region of the single-copy nuclear gene GAPDH, and an internal reference primer and a probe capable of identifying various common animal-derived components are designed in a conserved region of the single-copy nuclear gene GAPDH. As the target and the internal reference are located in the same gene and coexist in the same cell according to the proportion of 1: 1, quantitative deviation caused by gene copy number difference or expression fluctuation in a traditional two-step method is remarkably reduced. The detection strategy constructed by the invention has double advantages of specific recognition and broad-spectrum reference, is clear in quantitative basis and simple and convenient to operate, is suitable for rapid recognition and quantitative adulteration analysis of sheep-derived components in meat products, and has wide application prospects and supervision values.

The present disclosure relates to transgenic animals with nuclear genomes comprising knockouts of both alleles of an SLA-DQ gene and / or an SLA-DR gene. The disclosure provides transgenic pigs, transplant products from the transgenic pigs and xenotransplantation methods utilizing the transplant products for treatment of transplant rejection, especially in humans.

The present disclosure relates to transgenic animals with nuclear genomes comprising knockouts of both alleles of an SLA-DQ gene and / or an SLA-DR gene. The disclosure provides transgenic pigs, transplant products from the transgenic pigs and xenotransplantation methods utilizing the transplant products for treatment of transplant rejection, especially in humans.

Bottleneck Sequencing System (BotSeqS) is a next-generation sequencing method that simultaneously quantifies rare somatic point mutations across the mitochondrial and nuclear genomes. BotSeqS combines molecular barcoding with a simple dilution step immediately prior to library amplification. BotSeqS can be used to show age and tissue-dependent accumulations of rare mutations and demonstrate that somatic mutational burden in normal tissues can vary by several orders of magnitude, depending on biologic and environmental factors. BotSeqS has been used to show major differences between the mutational patterns of the mitochondrial and nuclear genomes in normal tissues. Lastly, BotSeqS has shown that the mutation spectra of normal tissues were different from each other, but similar to those of the cancers that arose in them.

The application discloses a method for identifying Ectropis oblique and Ectropis grisescens, and belongs to the technical field of molecular biology. The application takes Ectropis oblique and Ectropis grisescens as research objects, systematically screens diagnostic different sites in the whole genome based on population genome resequencing data, and develops a nucleic gene molecular marker which can be used for rapid and accurate identification of close species. COI Compared with the mitochondrial markers in the prior art, the method has wider applicability and higher genetic variation sensitivity at the species and population levels. The molecular identification method established in the application provides reliable technical support for accurate identification of close pests and application of the close pests in plant quarantine and monitoring.

The present application belongs to the technical field of biological information detection, and discloses a nuclear genome copy number variation detection method, which can identify whether the sample to be detected is a female sample. If yes, the half value of the sequencing depth value of the X chromosome site and the sequencing depth value of the autosomal site are compared with the respective confidence interval to determine the copy abnormal site, the copy number value of the copy abnormal site is calculated for spatial clustering classification, and the normal copy class and the copy variation class are obtained. The copy abnormal site belonging to the copy variation class is determined as the copy variation site. Then, the copy variation sites with adjacent positions and the same variation type are combined to obtain CNV fragments. Therefore, the present application can automatically and accurately perform CNV detection of all nuclear genome chromosomes, improve the accuracy of sex chromosome CNV detection, and improve the resolution of RD sites while ensuring the stability, accuracy and sensitivity of the CNV fragments.

The invention discloses application of a nuclear gene ITS sequence in identification and / or distinguishing of pokeberry root and counterfeit products thereof. The 1881st site of the nuclear gene ITS sequence with the GenBank login number of OL824854.1 is T or A, and the 1996 site nucleotide is T or C; or the nucleotide at the 1938th site is G or A, and the nucleotide at the 2031st site is T or A; the radix phytolaccae is Phytolacca aericana L. L., and the fake product is Phytolacca latinia (Moq.) Maxim., the radix phytolaccae is Phytolacca aericana L. L., and the fake product is Phytolacca latinia (Moq.) Maxim. According to the invention, a section of ITS bar code sequence capable of simultaneously identifying Phytolacca latbenia (Moq.) Maxim. In a sample to be detected is found for the first time, and the detection method designed based on the section of sequence is simple and reliable, is not influenced by the characters of medicinal materials, and can be used for accurately identifying medicinal material powder; the method has the advantages of high objectivity, no dependence on subjective judgment and the like.

The present invention provides a Coptidis species identification primer pair and a Coptidis species identification method, belonging to the field of molecular identification technology. The Coptidis species identification primer pair and the Coptidis species identification method provided by the present invention can overcome the problem of poor Coptidis species identification effect in the prior art. The identification region targeted by the Coptidis species identification primer pair provided by the present invention is a chloroplast fragment, not a nuclear gene fragment, and there is no multi-copy situation. At the same time, it can avoid the common microbial contamination problem of traditional Chinese medicine materials. The identification region fragment is short, with a length of only 400bp, which is much shorter than other DNA barcode fragments. Since degradation is a common phenomenon in traditional Chinese medicine materials, shorter barcode fragments are beneficial to improving the PCR amplification efficiency of degraded materials. It has strong practical application significance in Coptidis species identification.

The application discloses a Ganoderma lucidum double-nucleus genome assembly method based on haplotype analysis and belongs to the technical field of bioinformatics. The application aims to overcome the precision and accuracy of high-hybrid genome assembly. The application provides a Ganoderma lucidum double-nucleus genome assembly method based on haplotype analysis, obtains single-nucleus Ganoderma lucidum cells, respectively performs whole genome sequencing on the cells by using Illumina NovaSeq, Nanopore, Hi-C and PacBio HiFi, obtains precise genome information from Illumina NovaSeq sequencing data and Nanopore sequencing data, and realizes a haplotype separation assembly strategy.

Provided herein are methods for conferring cytoplasmic male sterility (CMS) on a plant line. The methods include obtaining a first plant comprising a CMS cytoplasm that is also a haploid inducer (CHIP) and crossing it with a second plant that comprises a desired nuclear genome (DIP). The CHIP also comprises a cenh3 mutation and may contain an anthocyanin marker and a restorer factor. The method further comprises generating progeny from said cross. The progeny produced from the cross of the method is haploid and comprises the CMS cytoplasm of the CHIP as well as the desired nuclear genome of the DIP. The progeny further lacks any anthocyanin marker or restorer factor.

Provided herein are methods and compositions for increasing production of a target protein or functional RNA by a cell.

The invention provides a construction method of an expression vector of eukaryotic genes in prokaryotes, and belongs to the field of molecular biology. The method comprises the following steps: (a) designing a PCR (Polymerase Chain Reaction) primer pair for amplifying each exon in a specific transcription spliceosome cds sequence; (b) respectively amplifying each exon by using the PCR primer pair designed in the step (a) to obtain a PCR fragment of each exon; (c) mixing the n exon fragments obtained in the step (b) as a template, and carrying out overlapping PCR by using the forward primer P1-F of the first exon and the reverse primer Pn-R of the final exon to obtain a complete cds fragment of the specific transcription spliceosome; and (d) purifying and recovering the cds fragment of the specific transcription spliceosome obtained in the step (c), and connecting the cds fragment to a prokaryotic expression vector. Therefore, the problem that the cds fragment of the target transcription spliceosome cannot be obtained due to unknown temporal-spatial expression information of a luxury gene or a specific transcript is solved.

This invention provides information on the potato StTuA and StTuB genes and their applications in improving late blight resistance, abiotic stress resistance, and yield. This invention discovers and confirms that two chloroplast elongation factors, StTuA and StTuB, encoded by potato nuclear genes and synthesized in the cytoplasm, can promote leaf photosynthesis, significantly increase potato tuber yield, and significantly enhance resistance to late blight and abiotic stress. Therefore, this study provides two key target genes for improving potato yield and resistance; increasing the expression levels of StTuA and StTuB genes can significantly improve potato yield and late blight resistance.

The invention discloses a single-gene hereditary cardiovascular disease related gene detection probe group, a kit and an application of the single-gene hereditary cardiovascular disease related gene detection probe group. The probe group is a specific probe which is selected from exon regions of genes such as ABCC9, ABCG8, ACADM, ACADS, ACADVL, ACTA2, ACTC1, ACTN2, ACVRL1, AGK, AGL, AGXT, AIFM1 or ALMS1 and the like, and 20-30 bp intron regions on the upstream and downstream of the exon regions of the genes such as ABCC9, ABCG8, ACADM, ACADDS, ACADVL, ACTA2, ACTC1, ACTN2, ACVRL1, AGK, AGL, AGXT, AIFM1 or ALMS1. The detection probe and the kit provided by the invention can accurately detect exon regions of 268 pathogenic gene variations related to the single-gene hereditary cardiovascular disease, intron regions of 20-30 bp upstream and downstream of exon and copy number variations of pathogenic genes, under the average 1G data volume, the nuclear genome coverage depth reaches 200 * on average, the 30 * coverage degree reaches 99%, and the detection sensitivity is high. The detection sensitivity of low-frequency mutation in a target area is improved, the detection cost is effectively controlled, an experimental system is stable, and the discrete coefficient of library results among samples is less than 10%.

The invention relates generally to a method of treatment for a human genetic disease, such as diseases characterized by unbalanced nucleotide pools, e.g., mitochondrial DNA depletion syndromes, and more specifically, thymidine kinase 2 (TK2) deficiency, using gene therapy. The gene therapy may involve administration of one or more constructs, such as a viral vector, containing a nucleic acid encoding a functional protein. The functional protein may correspond to a nuclear gene. For treatment of TK2 deficiency, the gene therapy may involve administration of one or more constructs, such as a viral vector, containing a nucleic acid encoding a functional TK2 enzyme. The treatment may also involve the administration of pharmacological therapy in conjunction with the gene therapy. The treatment protocols of the disclosure, such as those involving gene therapy alone or in combination with pharmacological therapy, can be used to treat, prevent, and / or cure various other disorders of unbalanced nucleoside pools, especially those found in mitochondrial DNA depletion syndrome.

The application discloses a quadruple real-time fluorescent PCR primer probe combination, product and identification method for identifying yak milk, yellow cattle milk, real yak milk and fake yak milk, and relates to the technical field of dairy product identification. The application comprises a first primer probe combination for detecting yak nuclear genes, a second primer probe combination for detecting yak mitochondrial genes, a third primer probe combination for detecting yellow cattle nuclear genes, and a fourth primer probe combination for detecting yellow cattle mitochondrial genes. The primer probe combination and the identification method can accurately identify yak milk, yellow cattle milk, real yak milk and fake yak milk. Meanwhile, the method can also accurately identify 1% of yak milk or cattle milk adulteration in yak milk, and can meet the needs of sample acquisition and market sample identification of yak milk adulteration.

The invention provides a green Chinese onion single-gene recessive genic male sterile line. The genic male sterile line is genic male sterile, and the phenotype of the genic male sterile line is that stamens are degraded and free of anther, and stigmas are large and similar to rods. The sterility of the green Chinese onion single-gene recessive genic male sterile line bred by the method is controlled by a pair of recessive genic genes, the genetic stability is realized, the stable sterility can be shown under different environmental conditions, and the problem that the sterility of some sterile lines in the prior art is easily influenced by the environment is solved.

To provide synthetic genomes in which one or more sense codons are removed, and to provide improved methods for producing synthetic genomes.SOLUTION: The present invention provides synthetic prokaryotic genomes that contain five or four or fewer occurrences of one or more sense codons; and / or synthetic prokaryotic genomes derived from parental genomes, the synthetic prokaryotic genome containing less than 10%, 5%, 2%, 1%, 0.5%, or less than 0.1% of the occurrences of one or more sense codons compared to the parental genome; and / or synthetic prokaryotic genomes that contain 100 or 101 or more, 200 or 201 or more, or 1,000 or 1,001 or more genes that lack one or more occurrences of sense codons.SELECTED DRAWING: None

The invention provides two binuclear gene locus homozygous ganoderma lucidum strains Ganoderma lucidum H1-1 and Ganoderma lucidum H1-2, which are both preserved in China Center for Type Culture Collection (CCTCC), the preservation address is No. 299, Wuchang District, Wuhan City, Hubei Province, the postal code is 430072, and the preservation numbers are respectively CCTCC: M2025856 and CCTCC: M 2025857. The invention also provides a preparation method of the binuclear gene locus homozygous ganoderma lucidum strain. The H1-1 and H1-2 dual-core gene locus homozygous ganoderma lucidum strain created by the invention can provide a new technical means for quickly and accurately verifying functions of ganoderma lucidum genes, and also can provide a new technical path for creating other types of edible mushroom gene locus homozygous strains.

The present invention relates to a breeding system for the production of polyploidy seeds, plants or plant parts in which cycles of meiosis, gamete coordination and selection are used for inter-population improvement of progenitor lines and sexual polyploidy occurs during hybrid production by inducing cloned gamete formation in parents to be hybridized. Mutual recurrent selection can be used to inform the selection of candidate lines that are advanced to a gene editing or genetic modification system, or hybridized and selected to induce clone gamete formation by preventing meiotic recombination and chromosomal subtrahend. Hybridization of parent plants carrying cloned gametes is planned and performed based on the predicted heterosis performance at the polyploidy level. The final product is a homogeneous population of hybrid polyploidy seeds or derivatives thereof, which carries the intact nuclear genome of both parents. In some cases, the methods are used to generate plants that produce seedless fruits.

The application discloses a Parkinson's disease cell model with mitochondrial damage, a construction method and application thereof. The construction method utilizes a target virus containing a CRISPR / Cas9 system to infect dopaminergic neuron cells, can precisely knock out a gene fragment coding mitochondrial transcription factor A in a nucleus gene of the dopaminergic neuron cells, makes the dopaminergic neuron cells reduce expression of TFAM protein, causes mitochondrial dysfunction, and further forms the Parkinson's disease cell model with mitochondrial damage. The mitochondrial damage feature of the Parkinson's disease cell model provided by the application is hereditary, therefore, after CRISPR / Cas9 gene editing is performed on primary cells, the edited primary cells are cultured to obtain a sufficient number of cell models for subsequent experiments and research. Compared with a method of exposing to rotenone, the method does not need to re-model each time the Parkinson's disease cell model is used, and the subcultured cells can be directly taken.