Method for obtaining male sterile lines of rice through fertility genes S44

A fertility gene and fertility technology, which is applied in plant hybrid breeding, fertility gene S44 and its mutants and its application in hybrid breeding, can solve the problem of limited resource utilization, substandard purity of hybrid seeds, and poor fertility. Vulnerable to external environmental factors

Pending Publication Date: 2019-06-18
SHENZHEN INST OF MOLECULAR CROP DESIGN
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  • Claims
  • Application Information

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Problems solved by technology

The three-line hybrid method requires specific restorer lines and maintainer lines, which not only complicates the seed production process, but also greatly limits the utilization of heterosis for different varieties of resources
[0005] The successful application of two-line hybridization technology in rice has made important contributions to increasing yield, quality, resistance and adaptability, and has important application v...

Method used

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  • Method for obtaining male sterile lines of rice through fertility genes S44
  • Method for obtaining male sterile lines of rice through fertility genes S44
  • Method for obtaining male sterile lines of rice through fertility genes S44

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Embodiment 1, the screening of rice male sterile mutant s44

[0104] The mutant was obtained by mutagenizing the seeds of indica rice Huanghuazhan (M 0 ) method, wherein EMS mutagenesis concentration and mutagenesis time are 0.7% and 12 hours respectively, will come from M 0 The plants of the generation seeds were harvested after fruiting to obtain the mutant library (M 1 ). from M 1 The plants of the generation seeds are used for screening at the seed maturity stage, and the sterile plants are obtained through phenotype observation. Sterile plants are regenerated by cutting rice piles, and the regenerated plants are regenerated with I 2 -KI staining was used to detect pollen development and staining reaction, and one of the mutants was found to be sterile without pollen grains (lodine loss), and it was named s44 mutant.

Embodiment 2

[0105] Embodiment 2, genetic analysis of rice male sterile mutant s44

[0106] The F 1 The generation plants all appeared to be fertile. F again 1 F 2 The generation plants can be clearly divided into two groups of fertile plants and sterile plants, and the segregation ratio of fertile plants and sterile plants is close to 3:1 (see Table 1), indicating that the sterility mutation trait of the s44 mutant is caused by Single recessive nuclear gene control.

[0107] Table 1 Rice male sterile mutant s44 hybrid F 2 Generation Separation Ratio

[0108]

Embodiment 3

[0109] Example 3, Analysis of reproductive organ phenotype of rice male sterile mutant s44

[0110] Compared with the wild-type Huanghuazhan, the s44 mutant grew normally, had a late heading, and the shape of the palea was significantly different from that of the wild type. The palea of ​​the mutant was crescent-shaped, the entire width was narrowed, and the length did not change significantly ( See figure 1 ), and the anthers of the mutant are thin, white and slightly transparent (see figure 2 ). further use I 2 The pollen of the mutant was stained and detected by -KI solution, and it was found that the pollen of the wild type was normally stained, while the mutant showed no pollen grains, that is, it was in a state of failure (see image 3 ). It was further found that the female organs (including ovary, style and stigma) of the mutant were slightly larger than those of the wild type (see Figure 4 ), the stigma exposure rate was more than 85%, while the stigma of the ...

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Abstract

The invention discloses a method for obtaining male sterile lines of rice through fertility genes S44, and belongs to the technical field of biology. The method specifically includes the steps that amale sterile mutant controlled by single recessive nuclear genes is obtained through EMS mutagenesis rice, mutant character related genes are positioned through a Mutmap method, and the rice fertilityregulatory genes S44 are obtained. Mutation of the S44 genes can lead to a male fertility phenotype produced by rice, the fertility of plants can be regulated by regulating the expression of the S44genes, and according to the method for obtaining the male sterile lines of the plants and regulating the plant fertility, the great significance is achieved on researching anther developmental mechanisms of the plants and crossbreeding work of the rice.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a plant hybrid breeding method, including sterile line propagation and hybrid seed preparation, and more specifically relates to a fertility gene S44 and its mutant and its application in hybrid breeding. technical background [0002] Hybrid breeding is an important method to improve the yield and quality of crops. Hybrids usually have obvious yield, resistance and adaptability advantages over conventional varieties. The breeding cycle of hybrids is usually shorter and quicker than conventional breeding. At present, hybrid breeding has become the main breeding method for many crops. [0003] The selection of crop male sterile lines is a key link in hybrid breeding. The male sterile line is a plant individual with defective male gamete development and normal female gamete development, and receives pollen from the male parent as the female parent. Several factors need to b...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H1/02
Inventor 严维陈竹锋唐晓艳
Owner SHENZHEN INST OF MOLECULAR CROP DESIGN
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