56 results about "Gene control" patented technology

The invention belongs to the field of gene engineering, and relates to a rice BRW1 gene and application thereof. The cDNA (complementary deoxyribonucleic acid) sequence of the rice BRW1 gene is shown as SEQ ID NO.1, and the amino acid sequence of the RNA binding protein coded by the rice BRW1 gene is shown as SEQ ID NO.2. The invention discloses the rice RNA binding protein gene BRW1 and the protein coded by the rice RNA binding protein gene BRW1. The rice BRW1 gene controls the grain weight of grains, the thousand grain weight of a knockout mutant of the gene is remarkably increased, and meanwhile the grain length and the grain width are increased. By mutating the BRW1 gene in the invention, the grain weight of rice grains can be increased, which indicates that the genetic improvement of the grain weight and grain shape of rice is facilitated by using the gene.

The present invention encompasses compositions and methods to allow one to deliver and express multiple genes under the control of multiple gene regulatory components in a recipient cell via a synthetic chromosome. The engineering of synthetic chromosomes to contain multiple gene control units permits the construction of complex biological circuits.

The invention provides application of a product of targeted Vasorin protein in preparation of a product for diagnosing or treating kidney diseases with reduced glucose tolerance, and belongs to the technical field of biological medicines. Vasorin is used as a biomarker of the hypoglucose tolerance nephropathy, the level of Vasorin in urine of a patient is absolutely quantified through an ELISA technology, and the Vasorin has great clinical value and practical significance in early discovery and intervention of the hypoglucose tolerance nephropathy. According to the present invention, the gene or the Vasorin protein for controlling the Vasorin expression is adopted as the target spot, and the specific vector is adopted to perform overexpression on the Vasorin protein, such that the lipid droplet deposition and the reactive oxygen species (ROS) overgeneration in the renal tubular epithelial cell can be effectively improved, and the albumin reabsorption ability of the renal tubular can be improved so as to effectively treat the glucose tolerance reduced nephropathy.

The invention discloses a CmMYC2 gene for regulating and controlling the color of sweet melon peel, an encoded protein and application, and belongs to the technical field of bioengineering breeding. In order to solve the technical problems of long period and high cost in sweet melon peel color identification in the prior art, the invention provides a CmMYC2 gene for regulating and controlling the sweet melon peel color, an encoded protein and application of the CmMYC2 gene in regulating and controlling the sweet melon peel color. The invention identifies and verifies a CmMYC2 gene participating in sweet melon pericarp color regulation, through field phenotypic identification, a BSA-seq technology is utilized to finely locate a gene MELO3C003412.2 for controlling the yellow / green pericarp traits of melons, and the gene MELO3C003412.2 is named as CmMYC2. The invention provides a solid theoretical technology for technicians in the field to screen and identify germplasm resources of different peel colors of muskmelons and molecular marker-assisted selection, and provides a new idea for new variety breeding.

The invention relates to the technical field of biology, in particular to a cell strain capable of expressing GlyR alpha1 / beta type GlyR and a preparation method of the cell strain. The cell strain contains heterologous nucleic acid stably integrated on a chromosome of a genome of the cell strain, and the method comprises the following steps: taking a mammalian cell strain as a host cell, and contacting the host cell with the heterologous nucleic acid; the heterologous nucleic acid comprises a) a first heterologous nucleic acid containing a CMV promoter and a GlyR alpha 1 gene controlled and started by the CMV promoter, and b) a second heterologous nucleic acid containing a Teton expression system and a GlyR beta gene induced and controlled by the Teton expression system.

To provide a novel approach for the expression or regulation of FOXP3 in a primary human lymphocyte.SOLUTION: Aspects of the invention described herein concern the incorporation of a FOXP3 cDNA (e.g., full-length of a human codon-optimized FOXP3 cDNA) into a FOXP3 gene or a non-FOXP3 locus so as to provide constitutive or regulated FOXP3 expression in a primary human CD34+ cells or cells derived from edited CD34+ cells. In some embodiments, guide RNA sequences that are directed to FOXP3, AAVS1, or other candidate loci are used for CRISPR / Cas9-mediated gene regulation, and gene delivery cassettes for homology-directed repair (HDR)-based gene-modification are provided.SELECTED DRAWING: Figure 1

Provided herein are KRN2 gene controlling kernel row number in plant, molecular markers closely linked to KRN2 and their application in molecular breeding.

The present invention discloses an IS26-based resistance gene control plasmid, engineering bacteria, bacterial agent and application, belonging to the technical field of genetic engineering. The nucleotide sequence of the IS26-based resistance gene control plasmid is shown in SEQ ID NO: 1 or SEQ ID NO: 2. The engineering bacteria and bacterial agent containing the resistance gene control plasmid can be used for the control of resistance genes. By specifically destroying the insertion sequence IS26, the number of resistance genes can be fundamentally reduced and the spread of resistance genes can be blocked, providing a precise solution for the treatment of drug resistance. It has broad-spectrum resistance bacteria control performance and can effectively control gentamicin, tetracycline, chloramphenicol and ampicillin-resistant bacteria in wastewater, with the highest control efficiency reaching 83.76%. The plasmid, engineering bacteria and bacterial agent have low preparation costs, can be used for in-situ treatment of wastewater, have low treatment costs and good effects, and have broad application prospects.

The invention belongs to the technical field of molecular markers, and particularly relates to a QTL gene for regulating and controlling maize fusarium graminearum stem rot resistance, a KASP molecular marker and application of the QTL gene and the KASP molecular marker. A maize high-disease-resistance inbred line H1710 and a high-susceptibility inbred line Huangzao 4 are used for constructing an F2 group, and high-disease-resistance and high-susceptibility samples are selected from the F2 group for constructing a mixed pool for BSA-seq analysis; in combination with bioinformatics analysis, molecular markers and fine positioning, a stable QTL gene for controlling the resistance of the fusarium graminearum stem rot of the corn is positioned from the sixth chromosome bin6.01 of the corn. On the basis, the invention provides a corn genome region capable of improving the corn stem rot resistance and a corresponding DNA (Deoxyribose Nucleic Acid) fragment thereof. In addition, the invention also provides a nuclear KASP molecular marker linked with the QTL locus. After the molecular marker is screened and verified, the SNP molecular marker provided by the invention can be used for efficiently and quickly identifying the genotype of a to-be-detected corn material and screening a stem rot resistant material.

This invention provides a gene related to the glossy leaf trait in Rugao black cabbage and its application, belonging to the field of molecular genetics and breeding technology. Specifically, this invention identifies the gene controlling the glossy leaf trait in 'Rugao black cabbage', confirms that this trait is controlled by a single dominant gene, and successfully locates the candidate gene. BraA02g02617P This study confirmed the association between the gene mutation and the glossy leaf trait. The discovery of the new dominant gene controlling glossy leaves greatly improves the convenience of molecular breeding of cruciferous plants, especially Brassica species, and has promising application prospects.

The invention discloses an application of CsABI5-5 gene in regulating citrus resistance to Huanglongbing and canker, and belongs to the field of genetic engineering technology. The invention obtains transgenic Ziyang orange hairy roots by vacuum infection of rhizobacterium rhizogenes to infect citrus stem segments with Huanglongbing, and finds that CsABI5-5 overexpressed transgenic hairy roots are resistant to the spread and proliferation of Huanglongbing bacteria CLas. In addition, by evaluating the canker resistance of overexpressed CsABI5-5 transgenic late Jin orange, it is found that the susceptible area of ​​transgenic late Jin orange is significantly smaller than that of late Jin orange of non-transgenic control group (WT) after inoculation with citrus canker bacteria Xcc for 10d, and its lesion area and disease index are significantly lower than those of WT group plants, indicating that overexpression of CsABI5-5 can improve the resistance of plants to citrus canker and significantly reduce the infection symptoms of Xcc. Lay a foundation for understanding the function of CsABI5-5 in citrus Huanglongbing and canker.

This invention relates to the field of biotechnology, specifically to the application of the ZmSKI3 and ZmSKI2 genes in regulating maize kernel development. This invention is the first to identify the ZmSKI3 and ZmSKI2 genes in maize and confirms that both genes control maize kernel development. Specifically, inhibiting the expression of ZmSKI3 and ZmSKI2 genes can delay maize kernel development. Therefore, this invention provides two key regulatory factors affecting maize kernel development, offering important molecular targets for improving maize kernel yield. Furthermore, this invention provides a novel gene resource for regulating maize kernel development; future manipulation of these genes can yield new germplasm with improved kernel development, enabling widespread application in production.

Disclosed are a plasmid comprising dxCas9 and a CRP derivative for controlling the expression of a target gene, a recombinant strain transformed with the plasmid, a preparation method therefor, a target gene control system, and a method for controlling the expression of a target gene, wherein the plasmid, recombinant strain, and expression control system according to the present invention can improve the production of high-value-added substances by construction of a system for simultaneously and multiply controlling the expression of target genes.

To provide methods and compositions that make it possible to construct complex biological expression control systems.SOLUTION: The present invention encompasses compositions and methods that make it possible to deliver and express multiple genes under the control of multiple gene regulatory components in a recipient cell via a synthetic chromosome. The engineering of synthetic chromosomes to contain multiple gene control units permits the construction of complex biological circuits.SELECTED DRAWING: None

The present invention discloses SSR molecular markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs, their primers and applications, belonging to the technical field of molecular genetics. The SSR molecular markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs disclosed by the present invention have amplification primer sequences as shown in SEQ ID NO.1 and 2. The SSR molecular markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs and their amplification primers can be applied to the population structure analysis of Corydalis bulbocapniodes, the analysis of markers associated with the fresh weight trait of Corydalis bulbocapniodes sub-bulbs, the selection of excellent germplasms of Corydalis bulbocapniodes and the preparation of a kit for screening excellent germplasms of Corydalis bulbocapniodes with high sub-bulb weight, and can be directly used for screening excellent germplasms, gene mapping and cloning, and molecular marker-assisted breeding. Moreover, they have chromosome mapping information, which is beneficial to further fine mapping and cloning of genes controlling the fresh weight trait of sub-bulbs, and is of great significance in the breeding of high-yield Corydalis bulbocapniodes.

The invention belongs to the technical field of biology, and relates to a method for increasing protein expression quantity by enhancing formation of mRNA (messenger ribonucleic acid) ribosome compounds. The method comprises the following steps: constructing a recombinant expression vector containing a first expression element of a target protein gene controlled by a first inducible promoter and a second expression element of a ribosome bS1 protein gene controlled by a second inducible promoter, introducing the recombinant expression vector into a recombinant bacterium, firstly inducing the expression of the target protein, and then inducing the expression of the ribosome bS1 protein, thereby obtaining the ribosome bS1 protein. Finally, the purpose of improving the expression of the target protein is achieved. Therefore, the invention can be used for improving the expression of the target protein and has wide application value.

The invention belongs to the field of plant genetic engineering, and discloses a grape transcription factor VvERF101 and application thereof in heat-resistant genetic improvement of plants, the VvERF101 gene is a transcription factor separated and cloned from Vitis labruca Baily Vitis vinifera L., and the sequence of the VvERF101 gene is as shown in SEQ ID NO.1. The invention further discloses a preparation method of the VvERF101 gene, and the VvERF101 gene is a transcription factor which is separated and cloned from the Vitis labruca Baily Vitis vinifera L.. The gene is used for constructing an over-expression vector, the over-expression vector is respectively introduced into tobacco through agrobacterium tumefaciens-mediated genetic transformation, and biological function verification of an obtained transgenic plant shows that the cloned VvERF101 gene has the function of controlling the heat resistance of the plant. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing environmental friendliness.

The present application relates to the field of plant growth and development molecular biology, and particularly relates to a gene OsARF14b and application thereof in controlling rice root elongation and tillering, the gene OsARF14b has the nucleotide sequence shown in SEQ ID NO:1, and the application of the gene OsARF14b in regulating rice root elongation and tillering traits.The present application obtains overexpression and knockout transgenic materials by cloning the gene OsARF14b, constructing a transgenic vector, and statistically analyzing the phenotype of the transgenic materials, and finds that the root length and tiller number in different materials change significantly, compared with the wild type (Zhonghua 11, ZH11), the root length of the knockout transgenic material (cas9) is significantly shortened, and the tiller number is significantly increased, the root length of the overexpression material (OE) is lengthened, and the tiller number is significantly reduced, which indicates that the OsARF14b gene plays an important regulatory function in the process of plant root elongation and tillering.

This invention discloses a wheat blue-grain gene and its applications, belonging to the fields of plant molecular biology, biochemistry, genetics, and plant breeding. Using differential expression analysis between blue-grain and white-grain wheat, four genes controlling the blue-grain trait were identified: two MYB family transcription factors and two bHLH family transcription factors. Furthermore, plant recombinant expression vectors for these genes and a method for regulating anthocyanin synthesis in plants are provided. This has significant theoretical and practical implications for studying the aleurone layer pigment synthesis pathway in blue-grain wheat, its use as a screening marker in plant transformation processes, and improving the nutritional value of plants.

A method of enhancing tolerance to drought stress in a plant includes inhibiting expression of a gene encoding a protein having the amino acid sequence of SEQ ID NO: 2 or genes encoding BCAT1 protein and BCAT2 protein from soybean having the amino acid sequence of SEQ ID NO: 32 and SEQ ID NO: 34, respectively in a plant cell.

The present application discloses a kind of Magnaporthe grisea and Xanthomonas oryzae resistance related protein derived from rice and its coding gene and application.The present application provides a kind of method for improving the disease resistance of plant: the gene of plant coding OsAspRS protein is replaced by the gene of mutant protein.The present application also protects the application of osasprs mutant in the preparation of plant with improved disease resistance.osasprs mutant is a kind of spot-like mutant plant controlled by single recessive nuclear gene, which is screened by EMS on Huanghuazhan plant.Furthermore, the present application successfully locates and clones AspRS gene, and the mutation of the gene site can cause spot-like phenotype on rice leaf, and further improve the broad-spectrum disease resistance of rice.The present application is helpful to improve the disease resistance of crop, provides gene resources and technical support for cultivating new rice varieties with broad-spectrum resistance, and has important significance and application value for crop disease resistance breeding work.

The application discloses a ZmSKI3 gene related to corn disease resistance and application thereof, and belongs to the technical field of biotechnology.The ZmSKI3 gene is identified in corn for the first time, and it is proved that the ZmSKI3 gene controls the resistance of corn to cercospora leaf spot disease, specifically, the expression of the ZmSKI3 gene is inhibited, and the resistance of corn to the cercospora leaf spot disease can be enhanced; the overexpression of the ZmSKI3 gene can weaken the resistance of corn to the cercospora leaf spot disease.The mutation of the ZmSKI3 gene obtained by the application can significantly activate the disease resistance immune response of corn, is a new corn disease resistance breeding gene resource, and provides an important molecular target for the selection of resistant corn.

Various examples relate to a transgenic crop plant where a sequence of a cis-regulatory element in the promoter of certain genes is genomically changed in order to alter the flowering time or flowering rate of the plant. In one example, the cis-regulatory element in the promoter of such certain genes may be modified from TGAATG(A / T) (A / T / C) to TGAGGG(A / T) (A / T / C). The certain genes may be genes that encode SbEhd1, OsFTL10 / FT8, SbFT10, ZmELF3.2, or combinations thereof. And, the crop plant can be for example, sorghum, maize or rice. In another example, the present transgenic crop plant is a progeny plant. In yet another example, flowering time of the transgenic crop plant is regulated by a method that includes changing the sequence of the cis-regulatory element in the promoter of certain genes.

The invention relates to the technical field of peanut breeding, in particular to a gene for controlling peanut root nodule organ formation and molecular marker application thereof. Comprising a gene NODULATING PEANUT 1a, which is called NNP1a for short; and a gene NON-NODULATINGPEANUT1b, which is called NNP1b for short, as well as a gene NON-NODULATINGPEANUT1b. Wherein the nucleotide sequence of the NNP1a is as shown in SEQ ID NO. 1, and the nucleotide sequence of the NNP1b is as shown in SEQ ID NO. 2. The gene for controlling the formation of the peanut root nodule is positioned, the molecular marker for detecting the NNP1a and NNP1b genes is constructed, the molecular marker is applied to detection of the NNP1a and NNP1b genes, and a feasible method is provided for detecting and controlling the formation of peanut root nodule organs.

The present invention provides a platform for regulating the expression of a target gene using exposure to an aptamer ligand (e.g., a small molecule) and methods of using the platform. The platform features a polynucleotide gene regulatory cassette that is placed in the target gene and includes a synthetic riboswitch placed in a 5' intron selective exon 3' intron context. The riboswitch comprises an effector region and a sensor region (e.g., an aptamer that binds a small molecule ligand) such that when the ligand is not present, the selective exon is spliced ​​into the target gene mRNA, thereby preventing the expression of the target gene. When the ligand is present, the selective exon is not spliced ​​into the target gene mRNA, thereby providing expression of the target gene.

The application provides application of a preparation targeting a Vasorin protein in preparation of a diagnosis or treatment product for impaired glucose tolerance kidney disease, and belongs to the technical field of biological medicine. Vasorin is used as a biomarker for impaired glucose tolerance kidney disease, and absolute quantification of the level of Vasorin in urine of patients through ELISA technology has great clinical value and practical significance for early discovery and intervention of impaired glucose tolerance kidney disease. Taking a gene controlling expression of Vasorin or the Vasorin protein as a target, overexpression of the gene or the protein by using a specific carrier can effectively improve lipid droplet deposition in renal tubular epithelial cells, excessive generation of reactive oxygen species (ROS), and the ability of the renal tubular epithelial cells to reabsorb albumin, so that effective treatment of impaired glucose tolerance kidney disease is achieved.

The present invention belongs to the field of plant genetic engineering technology. Specifically, it relates to the application of maize gene ZmEREB147 in controlling the flowering period of maize. The applicant has isolated a gene located on maize chromosome 3 that can control the flowering period of maize by using genetic methods. ZmEREB147 This gene controls important agricultural traits in maize, such as tasseling and pollen shedding. Reducing its expression level could advance the tasseling and pollen shedding periods, providing genetic resources and theoretical support for maize breeding.

The present invention relates to a genetically controlled nanoscopy contrast-generating unit comprising a metal interactor, wherein the metal interactor is compatible with nanoscopy fixation protocols, nanoscopy post-fixation protocols, and nanoscopy metal staining protocols and wherein the metal interactor is a molecule to which metal ions can bind to or react with. The present invention also relates to a genetically controlled structural element, wherein said genetically controlled structural element organizes the genetically controlled nanoscopy contrast-generating unit. The genetically controlled structural element can be an encapsulin and the genetically controlled nanoscopy contrast-generating unit can be one or two murine metallothionein-3, or three chimeric metallothioneins. The present invention also relates to a genetically controlled scaffold, wherein said genetically controlled scaffold spatially organizes the genetically controlled structural elements. The present invention also relates to the use of such genetically controlled nanoscopy contrast-generating unit, such genetically controlled structural element, and / or such genetically controlled scaffold for nanoscopy detection methods. The present invention also relates to a nanobiomaterial consisting of the isolated genetically controlled structural elements, and / or genetically controlled scaffolds. The present invention also relates to vectors comprising a nucleic acid encoding a genetically controlled nanoscopy contrast-generating unit, a genetically controlled structural element, and / or a genetically controlled scaffold.

The invention belongs to the technical field of plant genetic engineering, and relates to a rice HGR1 gene and application thereof in increasing rice grain weight. The invention discloses a rice HGR1 gene and a protein coded by the rice HGR1 gene, the genome nucleotide sequence of the HGR1 gene is shown as SEQ ID NO.1, the nucleotide sequence of a coding region of the HGR1 gene is shown as SEQ ID NO.2, and the sequence of the coded protein is shown as SEQ ID NO.3. The invention further discloses a preparation method of the rice HGR1 gene. The invention finds that the HGR1 gene controls the rice grain weight, knockout of the HGR1 gene enables the rice grain weight to be significantly increased, the grain length is increased, and grain filling is accelerated, which indicates that the HGR1 gene has potential utilization value in genetic improvement of the rice grain weight.

The present disclosure generally relates to engineered Cluster Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated (Cas) 12a proteins and system, and methods for use in gene editing and gene modulation for application to gene therapy. Related systems and methods of gene modulation are also disclosed.