32 results about "Gene control" patented technology

The present invention encompasses compositions and methods to allow one to deliver and express multiple genes under the control of multiple gene regulatory components in a recipient cell via a synthetic chromosome. The engineering of synthetic chromosomes to contain multiple gene control units permits the construction of complex biological circuits.

The invention provides application of a product of targeted Vasorin protein in preparation of a product for diagnosing or treating kidney diseases with reduced glucose tolerance, and belongs to the technical field of biological medicines. Vasorin is used as a biomarker of the hypoglucose tolerance nephropathy, the level of Vasorin in urine of a patient is absolutely quantified through an ELISA technology, and the Vasorin has great clinical value and practical significance in early discovery and intervention of the hypoglucose tolerance nephropathy. According to the present invention, the gene or the Vasorin protein for controlling the Vasorin expression is adopted as the target spot, and the specific vector is adopted to perform overexpression on the Vasorin protein, such that the lipid droplet deposition and the reactive oxygen species (ROS) overgeneration in the renal tubular epithelial cell can be effectively improved, and the albumin reabsorption ability of the renal tubular can be improved so as to effectively treat the glucose tolerance reduced nephropathy.

The invention discloses a CmMYC2 gene for regulating and controlling the color of sweet melon peel, an encoded protein and application, and belongs to the technical field of bioengineering breeding. In order to solve the technical problems of long period and high cost in sweet melon peel color identification in the prior art, the invention provides a CmMYC2 gene for regulating and controlling the sweet melon peel color, an encoded protein and application of the CmMYC2 gene in regulating and controlling the sweet melon peel color. The invention identifies and verifies a CmMYC2 gene participating in sweet melon pericarp color regulation, through field phenotypic identification, a BSA-seq technology is utilized to finely locate a gene MELO3C003412.2 for controlling the yellow / green pericarp traits of melons, and the gene MELO3C003412.2 is named as CmMYC2. The invention provides a solid theoretical technology for technicians in the field to screen and identify germplasm resources of different peel colors of muskmelons and molecular marker-assisted selection, and provides a new idea for new variety breeding.

Provided herein are KRN2 gene controlling kernel row number in plant, molecular markers closely linked to KRN2 and their application in molecular breeding.

The invention belongs to the technical field of molecular markers, and particularly relates to a QTL gene for regulating and controlling maize fusarium graminearum stem rot resistance, a KASP molecular marker and application of the QTL gene and the KASP molecular marker. A maize high-disease-resistance inbred line H1710 and a high-susceptibility inbred line Huangzao 4 are used for constructing an F2 group, and high-disease-resistance and high-susceptibility samples are selected from the F2 group for constructing a mixed pool for BSA-seq analysis; in combination with bioinformatics analysis, molecular markers and fine positioning, a stable QTL gene for controlling the resistance of the fusarium graminearum stem rot of the corn is positioned from the sixth chromosome bin6.01 of the corn. On the basis, the invention provides a corn genome region capable of improving the corn stem rot resistance and a corresponding DNA (Deoxyribose Nucleic Acid) fragment thereof. In addition, the invention also provides a nuclear KASP molecular marker linked with the QTL locus. After the molecular marker is screened and verified, the SNP molecular marker provided by the invention can be used for efficiently and quickly identifying the genotype of a to-be-detected corn material and screening a stem rot resistant material.

This invention provides a gene related to the glossy leaf trait in Rugao black cabbage and its application, belonging to the field of molecular genetics and breeding technology. Specifically, this invention identifies the gene controlling the glossy leaf trait in 'Rugao black cabbage', confirms that this trait is controlled by a single dominant gene, and successfully locates the candidate gene. BraA02g02617P This study confirmed the association between the gene mutation and the glossy leaf trait. The discovery of the new dominant gene controlling glossy leaves greatly improves the convenience of molecular breeding of cruciferous plants, especially Brassica species, and has promising application prospects.

This invention relates to the field of biotechnology, specifically to the application of the ZmSKI3 and ZmSKI2 genes in regulating maize kernel development. This invention is the first to identify the ZmSKI3 and ZmSKI2 genes in maize and confirms that both genes control maize kernel development. Specifically, inhibiting the expression of ZmSKI3 and ZmSKI2 genes can delay maize kernel development. Therefore, this invention provides two key regulatory factors affecting maize kernel development, offering important molecular targets for improving maize kernel yield. Furthermore, this invention provides a novel gene resource for regulating maize kernel development; future manipulation of these genes can yield new germplasm with improved kernel development, enabling widespread application in production.

The present application relates to the field of plant growth and development molecular biology, and particularly relates to a gene OsARF14b and application thereof in controlling rice root elongation and tillering, the gene OsARF14b has the nucleotide sequence shown in SEQ ID NO:1, and the application of the gene OsARF14b in regulating rice root elongation and tillering traits.The present application obtains overexpression and knockout transgenic materials by cloning the gene OsARF14b, constructing a transgenic vector, and statistically analyzing the phenotype of the transgenic materials, and finds that the root length and tiller number in different materials change significantly, compared with the wild type (Zhonghua 11, ZH11), the root length of the knockout transgenic material (cas9) is significantly shortened, and the tiller number is significantly increased, the root length of the overexpression material (OE) is lengthened, and the tiller number is significantly reduced, which indicates that the OsARF14b gene plays an important regulatory function in the process of plant root elongation and tillering.

This invention discloses a wheat blue-grain gene and its applications, belonging to the fields of plant molecular biology, biochemistry, genetics, and plant breeding. Using differential expression analysis between blue-grain and white-grain wheat, four genes controlling the blue-grain trait were identified: two MYB family transcription factors and two bHLH family transcription factors. Furthermore, plant recombinant expression vectors for these genes and a method for regulating anthocyanin synthesis in plants are provided. This has significant theoretical and practical implications for studying the aleurone layer pigment synthesis pathway in blue-grain wheat, its use as a screening marker in plant transformation processes, and improving the nutritional value of plants.

The present application discloses a kind of Magnaporthe grisea and Xanthomonas oryzae resistance related protein derived from rice and its coding gene and application.The present application provides a kind of method for improving the disease resistance of plant: the gene of plant coding OsAspRS protein is replaced by the gene of mutant protein.The present application also protects the application of osasprs mutant in the preparation of plant with improved disease resistance.osasprs mutant is a kind of spot-like mutant plant controlled by single recessive nuclear gene, which is screened by EMS on Huanghuazhan plant.Furthermore, the present application successfully locates and clones AspRS gene, and the mutation of the gene site can cause spot-like phenotype on rice leaf, and further improve the broad-spectrum disease resistance of rice.The present application is helpful to improve the disease resistance of crop, provides gene resources and technical support for cultivating new rice varieties with broad-spectrum resistance, and has important significance and application value for crop disease resistance breeding work.

The application discloses a ZmSKI3 gene related to corn disease resistance and application thereof, and belongs to the technical field of biotechnology.The ZmSKI3 gene is identified in corn for the first time, and it is proved that the ZmSKI3 gene controls the resistance of corn to cercospora leaf spot disease, specifically, the expression of the ZmSKI3 gene is inhibited, and the resistance of corn to the cercospora leaf spot disease can be enhanced; the overexpression of the ZmSKI3 gene can weaken the resistance of corn to the cercospora leaf spot disease.The mutation of the ZmSKI3 gene obtained by the application can significantly activate the disease resistance immune response of corn, is a new corn disease resistance breeding gene resource, and provides an important molecular target for the selection of resistant corn.

Various examples relate to a transgenic crop plant where a sequence of a cis-regulatory element in the promoter of certain genes is genomically changed in order to alter the flowering time or flowering rate of the plant. In one example, the cis-regulatory element in the promoter of such certain genes may be modified from TGAATG(A / T) (A / T / C) to TGAGGG(A / T) (A / T / C). The certain genes may be genes that encode SbEhd1, OsFTL10 / FT8, SbFT10, ZmELF3.2, or combinations thereof. And, the crop plant can be for example, sorghum, maize or rice. In another example, the present transgenic crop plant is a progeny plant. In yet another example, flowering time of the transgenic crop plant is regulated by a method that includes changing the sequence of the cis-regulatory element in the promoter of certain genes.

The application provides application of a preparation targeting a Vasorin protein in preparation of a diagnosis or treatment product for impaired glucose tolerance kidney disease, and belongs to the technical field of biological medicine. Vasorin is used as a biomarker for impaired glucose tolerance kidney disease, and absolute quantification of the level of Vasorin in urine of patients through ELISA technology has great clinical value and practical significance for early discovery and intervention of impaired glucose tolerance kidney disease. Taking a gene controlling expression of Vasorin or the Vasorin protein as a target, overexpression of the gene or the protein by using a specific carrier can effectively improve lipid droplet deposition in renal tubular epithelial cells, excessive generation of reactive oxygen species (ROS), and the ability of the renal tubular epithelial cells to reabsorb albumin, so that effective treatment of impaired glucose tolerance kidney disease is achieved.

The present invention belongs to the field of plant genetic engineering technology. Specifically, it relates to the application of maize gene ZmEREB147 in controlling the flowering period of maize. The applicant has isolated a gene located on maize chromosome 3 that can control the flowering period of maize by using genetic methods. ZmEREB147 This gene controls important agricultural traits in maize, such as tasseling and pollen shedding. Reducing its expression level could advance the tasseling and pollen shedding periods, providing genetic resources and theoretical support for maize breeding.

The invention belongs to the technical field of plant genetic engineering, and relates to a rice HGR1 gene and application thereof in increasing rice grain weight. The invention discloses a rice HGR1 gene and a protein coded by the rice HGR1 gene, the genome nucleotide sequence of the HGR1 gene is shown as SEQ ID NO.1, the nucleotide sequence of a coding region of the HGR1 gene is shown as SEQ ID NO.2, and the sequence of the coded protein is shown as SEQ ID NO.3. The invention further discloses a preparation method of the rice HGR1 gene. The invention finds that the HGR1 gene controls the rice grain weight, knockout of the HGR1 gene enables the rice grain weight to be significantly increased, the grain length is increased, and grain filling is accelerated, which indicates that the HGR1 gene has potential utilization value in genetic improvement of the rice grain weight.

The present disclosure generally relates to engineered Cluster Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated (Cas) 12a proteins and system, and methods for use in gene editing and gene modulation for application to gene therapy. Related systems and methods of gene modulation are also disclosed.

This invention discloses a gene controlling spike length and grain number per spike in wheat. This gene is located on chromosome 2B using a recombinant inbred line population and high-density SNP markers, within the 657.82–664.40 Mb region of chromosome 2B in the wheat Chinese spring reference genome. A candidate gene, named TaeEF1A, was identified based on the wheat reference genome sequence, RNA-seq, and gene function. Gene editing of TaeEF1A further validated its function in regulating spike length, spikelet number, and grain number per spike. Through gene sequencing and analysis of the gene sequence in a large number of germplasm resources, key variations affecting spike length and grain number per spike were identified in the 3'-UTR of TaeEF1A. Based on this, a detection marker was designed for this gene, which can be used to detect superior allelic variants of the TaeEF1A gene. This marker can be easily, rapidly, and with high throughput applied to marker-assisted breeding to increase spike length, spikelet number, and grain number per spike, thereby increasing yield.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a rice GW7.2 gene and application thereof in regulation and control of rice grain weight and grain length. The invention discloses a rice GW7.2 gene and a protein coded by the rice GW7.2 gene, the genome nucleotide sequence of the GW7.2 gene is shown as SEQ ID NO.1, the nucleotide sequence of a coding region of the GW7.2 gene is shown as SEQ ID NO.2, and the amino acid sequence of the coded protein is shown as SEQ ID NO.3. The invention further discloses a preparation method of the rice GW7.2 gene. The invention provides a technology for increasing the grain weight and the grain length of rice by using a rice gene GW7.2, and finds that the GW7.2 gene controls the grain weight and the grain length of the rice, and knockout of the GW7.2 gene enables the grain weight of the rice to be remarkably increased and the grain length to be remarkably increased, so that the GW7.2 gene has potential utilization value in genetic improvement and molecular breeding of the grain weight and the grain shape of the rice.

The application discloses a fine regulation method for rice heading stage. The method is based on a double gene mutant ygl219 for controlling yellow-green leaves and early heading. The yellow-green leaf phenotype is caused by mutation of a key enzyme of chlorophyll synthesis, ethylene reductase gene OsDVR (LOC_Os03g22780) (SEQ ID NO: 3 and SEQ ID NO: 4), and the early heading (10-15 days earlier than wild type Quantfeng B) phenotype is controlled by another unknown gene. The application combines the ygl219 mutant background with overexpression of the OsDVR coding sequence driven by the Ubi promoter, restores the yellow-green leaf and plant height phenotypes of the mutant, and specifically retains the early heading characteristics, and finally obtains improved rice materials with advanced heading stage and increased yield. The method provides a new technical approach for fine regulation of rice growth period and creation of early-maturing and high-yield parents, and has important application value for wide adaptability and simple cultivation of rice.

The invention provides a green Chinese onion single-gene recessive genic male sterile line. The genic male sterile line is genic male sterile, and the phenotype of the genic male sterile line is that stamens are degraded and free of anther, and stigmas are large and similar to rods. The sterility of the green Chinese onion single-gene recessive genic male sterile line bred by the method is controlled by a pair of recessive genic genes, the genetic stability is realized, the stable sterility can be shown under different environmental conditions, and the problem that the sterility of some sterile lines in the prior art is easily influenced by the environment is solved.

The invention belongs to the technical field of plant genetic engineering, and discloses application of a rice OsHT gene in improvement of drought resistance of rice. Clustered regularly interspaced short palindromic repeats (CRISPR) mutant phenotype identification is carried out on candidate genes, and the OsHT gene for controlling rice drought response is successfully cloned. The drought stress phenotype identification results in the seedling stage and the adult-plant stage show that when the gene segment is deleted, the drought stress resistance of the rice is obviously enhanced, so that the function of the gene and the potential of the gene in practical application are proved.

The invention discloses a gene for controlling plant branching and application thereof. According to the invention, a new gene OsDIP8 for regulating and controlling the growth and development of rice tillering is screened and cloned in rice for the first time, and after the gene is over-expressed, the number of rice tillering is remarkably increased, which proves that the gene has a positive regulation and control effect on the important agronomic trait, namely the number of rice tillering. According to the invention, the important regulation effect of the molecular chaperonin on the important agronomic trait tillering number of rice is identified for the first time, and new genes and germplasm resources are provided for deep research of a plant branching or tillering regulation mechanism and rice plant type breeding.

The application discloses a ctPistol ribozyme and application thereof in gene silencing, and belongs to the technical field of biology.The disclosed ctPistol ribozyme can realize effective control of cell phenotype by silencing the expression of key genes.In addition, when applied in a mouse cancer model, the ctPistol realizes a powerful and persistent anti-tumor effect by targeting oncogenic driver genes.As a powerful gene silencing tool and a promising RNA-based therapeutic candidate drug, the ctPistol ribozyme shows an effective way of developing diversified ribozyme-mediated gene control technology.

The invention belongs to the field of biological medicine, and particularly discloses a reversible immortalized human prostate cancer fibroblast and a construction method thereof. The method comprises the following steps: introducing an SV40 large T antigen gene controlled by a tetracycline response element into primary prostate cancer fibroblasts through a lentiviral vector, and carrying out puromycin screening and monoclonal culture to obtain the reversible immortalized cell line. The cell line can efficiently express the SV40 large T antigen under the induction of doxycycline, and infinite proliferation is realized; after the inducer is removed, the cell cycle is quitted, the fibroblast enters an aging state again, and specific marker expression of the fibroblast is kept all the time. The cell line can be used for prostate cancer tumor microenvironment research, high-throughput drug screening and matrix-epithelial cell interaction mechanism research, solves the problems of limited primary cell proliferation, large batch-to-batch difference and the like, and has good stability and reversible regulation.

To provide function-deficient transcription factor-like genes and inbreeding Fagopyrum plants using the same.SOLUTION: The function of transcription factor-like genes formed by a group of linked genes governing the length of the male core and the female core of a Fagopyrum plant identified from the result of decoding the genome sequence of the Fagopyrum plant is deleted by mutation induction to produce S-ELF3-PS1 genes which are function-deficient transcription factor-like genes, and an individual having the S-ELF3-PS1 inbreeding genes is cross-cultivated to grow a inbreeding Fagopyrum plant having long male core and female core.SELECTED DRAWING: Figure 1

This application relates to the field of molecular markers, specifically to molecular markers related to the easy separability of mature fruit and pedicel in chili peppers, their applications, and detection methods. This invention locates the gene controlling the easy separability of mature fruit and pedicel to chromosome 10, obtaining candidate regions for this gene. Molecular markers closely linked to this trait are then developed within these candidate regions. The invented molecular markers are closely linked to the gene controlling the easy separability of mature fruit and pedicel in chili peppers, exhibiting high identification efficiency. The development and utilization of these markers allows for the identification of whether the fruit and pedicel are easily separable even at the seedling stage.

The invention discloses application of soybean GmCDC7 protein and a coding gene thereof in regulation and control of soybean quality traits. The GmCDC7 gene encodes a plant germination-like protein, and the amino acid sequence of the GmCDC7 gene is as shown in SEQ ID No.2. According to the invention, gene editing is carried out on the GmCDC7 gene of a receptor plant Jack by using a CRISPR-Cas9 technology, and a homozygous mutant of the GmCDC7 gene is obtained. Compared with a wild type, the soybean seed protein content of a GmCDC7 gene mutant plant is remarkably increased, and the oil content is remarkably reduced. The result shows that the GmCDC7 gene negatively regulates and controls the protein content of the soybean seeds and influences the oil content of the seeds at the same time. The invention discloses the biological function of the soybean GmCDC7 gene for controlling the quality traits of soybean seeds for the first time.

This application discloses a novel algorithm for constructing a genetic map of a MAGIC population, comprising: collecting species data; segmenting chromosomes based on the collected data to form chromosome fragments; calculating the parental genetic similarity index of chromosome fragments to determine parental origin; obtaining a definite origin matrix and a fuzzy origin matrix; using definite fragments from a single parental origin in the definite origin matrix as anchor points; using the anchor points as starting points, examining adjacent fragments of fuzzy fragments in the fuzzy origin matrix to determine parental origin; merging fragments with the same parental origin and adjacent positions into a larger fragment to form a parental origin matrix; calculating recombination rate and genetic distance; constructing a genetic map; and using the novel algorithm to analyze the whole-genome resequencing data of a MAGIC population of a specified crop, thereby effectively detecting minor genes controlling complex quantitative traits, clarifying the parental origin of each variation site, and finding the required high-quality parents, thereby improving the breeding process.

The application discloses a QTL for controlling the plant height of wheat, a candidate gene TaDHL-7B and application. The QTL is QPh-7B-1242, is located on the 7B chromosome, and the genetic distance is 1234.5-1252.5 cM; and the candidate gene is TaDHL-7B, and the nucleotide sequence is shown as SEQ ID No. 1. The application carries out gene editing on the candidate gene TaDHL-7B, and it is verified through experiments that the TaDHL-7B gene controls the plant height of wheat, is a constitutive expression and relatively conservative gene, which is beneficial to the genetic improvement and molecular breeding of the plant height of wheat, and can accelerate the screening of dwarf wheat varieties or be used for regulating the plant height of wheat.

This invention relates to the field of molecular markers for peppers, and discloses an Indel molecular marker for identifying genes controlling light-induced purple color in pepper pericarps and its application. The applicant used pooled transcriptome sequencing (BSR-seq) combined with linkage mapping to identify the relevant sites controlling light-induced purple color in pepper pericarps, and designed the marker A_836 and detection primers based on the inserted Indel. By detecting the deletion or insertion of the provided specific fragment, the fruit color of peppers can be accurately and rapidly detected at the seedling stage, eliminating the need to wait until the fruit setting stage. This reduces labor, significantly improves breeding efficiency, and lowers breeding costs.