223 results about "Gene control" patented technology

The invention relates to the modulation of gene expression in a cell, also called gene control, in particular in relation to the treatment of anthrax. The invention provides a method for modulating expression of a gene in a cell comprising providing the cell with a signaling molecule comprising a peptide or functional analogue thereof.

The invention discloses a gene control device and method based on CRISPR-Cas9. The device comprises a nucleotide sequence, wherein the nucleotide sequence is an sgRNA itself or can be transcribed into an sgRNA, the sgRNA structurally comprises a first part bonded with inactivated Cas9 protein and a second part connected to the 3'end of the first part, the 5'end of the first part is provided with an antisense sequence, and the second part is an aptamer sequence part which comprises a ligand binding part and an aptamer stem part; when no ligand exists, the aptamer sequence is pair-bonded with the aptamer stem part; when a ligand exists, the aptamer sequence is dissociated from the aptamer stem part so as to be pair-bonded with a target DNA. By integrating an RNA riboswitch bonded with a specific biological signal into the sgRNA, a bridge of cell signal intensity and gene expression intensity is established, any target gene can be activated or silenced on the basis that biological signal identification is achieved, and artificial connection is built between signal ligands and cytogene.

The invention relates to the modulation of gene expression in a cell, also called gene control, in particular in relation to the treatment of a variety of diseases. The invention provides a method for modulating expression of a gene in a cell comprising providing the cell with a signaling molecule comprising a peptide or functional analogue thereof. Furthermore, the invention provides a method for identifying or obtaining a signaling molecule comprising a peptide or functional derivative or analogue thereof capable of modulating expression of a gene in a cell comprising providing the cell with a peptide or derivative or analogue thereof and determining the activity and/or nuclear translocation of a gene transcription factor.

The invention relates to a genetically engineered bacterium producing tetrahydropyrimidine and a structuring method and application thereof. The genetically engineered bacterium is Escherichia coli with a specific genotype, comprises Halomonas-elogata-derived ectABC gene, has three gene deficiency variants including lysA, thrA and iclR and has corynebacterium glutamicum lysC gene controlled by a lac promoter and ppc gene controlled by a trc promoter. The defect that chemical synthesis methods have harsh reaction conditions and high energy consumption can be overcome by using the genetically engineered bacterium and utilizing glucose fermentation to produce tetrahydropyrimidine. The defect that Halomonas-elogata fermentation or enzyme-catalyzed methods are complex in process and high in production cost can be overcome. After fermentation for 20-28h, yield of tetrahydropyrimidine reaches 12-18g/L, and the genetically engineered bacterium has high industrial application value.

The invention discloses recombinant bacillus subtitles capable of increasing the yield of acetylglucosamine and belongs to the field of genetic engineering. The recombinant bacillus subtitles is characterized in that bacillus subtitles BSGNKAP-Pxy1A-glmS-P43-GNA1 is used as the original strain, a phosphatase yqaB gene controlled by a strong constitutive promoter Pveg is integrated to the genome, and the promoter of 6-phosphate glucosamine synthase is replaced by the strong constitutive promoter Pveg to obtain genetically engineered bacillus subtitles BSGNY-Pveg-glmS-P43-GNA1 accumulating the acetylglucosamine. The recombinant bacillus subtitles has the advantages that the yield of the acetylglucosamine of a 3L fermentation tank reaches 60.5g/L, the yield of the acetylglucosamine is 0.311g/g glucose, production intensity is 0.983g/L/h, the yield of the acetylglucosamine outside the cells of the recombinant bacillus subtitles is increased, and a foundation is laid for glucosamine production using metabolic engineering modified bacillus subtitles.

The invention discloses a magnetic resonance-based water treatment device. The magnetic resonance-based water treatment device realizes water treatment through electromagnetic waves produced by magnetic resonance. The magnetic resonance-based water treatment device is characterized in that a power signal is subjected to transformation rectification by a transformation rectification circuit and then is input into a high-frequency inverter and a low-frequency inverter; radio frequency pulses having different frequency values are invertedly output by the high-frequency inverter and the low-frequency inverter; and the radio frequency pulses having different frequency values are transmitted to corresponding electromagnetic vibration wave transducers in a water treatment tank so that two different electromagnetic wave energy zones are formed. Through the magnetic resonance-based water treatment device, a water molecule is stimulated by resonance-supported radio frequency pulse energy waves and then splits and thus a hydrogen bond active force, an organization structure and an energy load capacity of water are changed. Water treated by the magnetic resonance-based water treatment device has the advantages that the bioavailability and the biological activity are improved; metabolism quality is improved; a free radical inhibition and removal capability is obtained; and a gene control capability is improved. The magnetic resonance-based water treatment device can produce health water, adopts a physical production technology and simple production processes, and has high production efficiency and a low cost.

The present invention relates to the development of a positive selection vector based on insertional reconstruction of a reporter gene or of a regulatory gene controlling the expression of a reporter gene. The cloning vector carries a reporter gene or a regulatory gene with a mutation rendering the reporter or the regulatory gene protein functionally inactive. A primer carrying a nucleic acid sequence that corrects the mutation is used during PCR amplification of a targeted nucleic acid sequence, and the amplified DNA fragment is then ligated to the said vector thus reconstructing the wild-type reporter or regulatory gene.

The invention discloses a continuous recessive character backcrossing technology which is characterized by taking a material which contains no target recessive genes and is ready to be improved as a recurrent parent, and taking a material which contains the target recessive genes as a donor parent; taking the recurrent parent as the parent to be hybridized with the donor parent and harvesting hybrid seeds of the first generation F1 and recurrent parent seeds; and planting the hybrid seeds of the first generation F1 and the recurrent parent seeds till the planting of a group containing backcross hybrid seeds of the second of n generations BCn F2 and till the typing into a target recessive gene isogenic line of the recurrent parent. The invention also discloses a method for creating a purpleleaf marker isogenic line by applying the continuous recessive character backcrossing technology and also a method for transferring new purple leaf marker nucleus sterile line by taking the purple leaf marker isogenic line as an intermediate material. In the methods, a selfing process is combined with a next backcrossing process for the treatment in one season, therefore, the time efficiency is improved by about 50 percent, a small number of dominant-gene-controlled unsuitable characters of existing fine-quality parents can be fast improved, and useful recessive characters can be fast imported to the present high-level heredity background.

The invention discloses a method for improving erythrocin yield through a negative control gene SACE_3446 on an inactivation saccharopolyspora erythraea chromosome. Saccharopolyspora erythraea is used for producing erythrocin. The erythrocin and derived drugs of the erythrocin such as clarithromycin, azithromycin and telithromycin are used widely in clinic. Erythrocin high-producing strain screening is very important in industrial production. The erythromycin biosynthesis negative control gene SACE_3446 is screened from a saccharopolyspora erythraea TetR family. Compared with erythrocin yield of an original strain, deletion mutants of the saccharopolyspora erythraea SACE_3446 is improved remarkably, the erythrocin is returned to low yield after gene complementation of the SACE_3446, and therefore the SACE_3446 gene is a erythromycin biosynthesis negative control gene. The inactivation saccharopolyspora erythraea SACE_3446 gene can improve the erythrocin yield through a genetic engineering way. Due to the fact that erythromycin biosynthesis gene control is a network system, upstream and downstream control factors acted by SACE_3446 control factors can be found by using the SACE_3446 as an object. The erythrocin yield can also be improved by changing upstream or downstream control factor genes of the saccharopolyspora erythraea SACE_3446 control factors.

The present invention provides an isolated nucleic acid including a nucleotide sequence encoding COL8, which controls hypocotyl length and flowering time in a plant. It is found that plants over-expressing COL8 under long day conditions show slight hypocotyl elongation and delayed flowering in comparison with the wild type. In addition, COL8 and FKF1, which is a circadian-clock related gene, are found to be localized at the same site in a plant cell, indicating the existence of an interaction between the two.

The invention relates to a bicistronic mRNA coexpression gene transporter and a preparation method thereof. The gene order of the gene transporter is SEQ ID No. 15; the gene transporter from 5' end to 3' end comprises bovine Nuclear matrix binding region MAR, artificially constructed combined promoter CAG, Profilin gene FSTN, internal ribosome entry site (IRES), green fluorescent protein AcGFP gene and Rabbit globin poly A signal region; the preparation method comprises the following steps: constructing a carrier pCAG-IRES2-AcGFP1; obtaining FSTN gene and inserting the pCAG-IRES2-AcGFP1 carrier; obtaining the sequence of MAR and inserting the pCAG-IRES2-AcGFP1 carrier; constructing a plasmid carriers so as to obtain the bicistronic mRNA coexpression gene transporter. The gene transporter is not only pure and safe gene transporter but also realizes dual-gene coexpression in any combination, achieves the purpose of multi-gene coexpression through repeated utilization of IRES, and provides new thinking and route for improving the shape of dual or multiple gene control such as economic character.

The invention discloses a rice bacterial leaf blight resistance related gene OsABA2 which consists of nucleotide sequences of SEQ ID No.2 as shown in the specification. The invention further discloses a rice lesion mimic gene which is capable of remarkably improving the resistance of rice to bacterial leaf blight, and a lesion mimic mutant can be acquired through gene edition on the OsABA2 gene. Novel functions of the OsABA2 gene are discovered, and the lesion mimic mutant acquired after the OsABA2 gene is knocked off through gene edition can be applied to rice bacterial leaf blight resistance breeding; meanwhile, the lesion mimic trait is controlled by a single recessive nuclear gene, and hybrid identification and selection can be relatively simple to implement in transgenosis or crossbreeding; and in addition, the lesion mimic gene is derived from nonglutinous rice and has a great promotion function on bacterial leaf blight resistant breeding of the nonglutinous rice.

A method for identifying resistance of a rice plant to rice blast is provided. More particularly, a method for identifying resistance of a rice plant to rice blast by testing a genotype of the rice genome using a DNA marker (G271), which is closely linked to a gene controlling the field resistance to rice blast, is disclosed. The disclosed invention allows for evaluation of the field resistance of a rice plant to rice blast using a DNA marker, thereby allowing for a resistant variety to be conveniently and accurately selected. The disclosed invention contributes to reducing the time and labor that have conventionally been required for cross breeding, and is useful in developing novel rice varieties having a high degree of field resistance to rice blast.

The invention discloses a cell line ST/T7 capable of stably expressing T7RNA polymerase, the microorganism preservation number of which is: CGMCC No.24444. In the invention, a T7RNA polymerase gene is inserted into a eukaryotic expression vector pIRES2-EGFP to get the pIRES2-EGFP-T7 to transfect ST cells to obtain the cell line ST/T7 capable of stably expressing T7RNA polymerase. Through RT-PCR detection, expression plasmid containing a red fluorescent protein gene controlled by T7 promotors can be used to transfect the cell line so as to observe the expression of red fluorescent protein in a transcription product amplified form a ST/T7 cell to the T7RNA polymerase. The cell line ST/T7 can provide reverse T7RNA polymerase for use in reverse genetic manipulation of RNA-virus such as hog cholera virus, etc., and can be used as transcription and expression systems in vitro for research on genic structures and functions.

The invention discloses a prodenia litura gene engineering virus No.3 and a building method thereof, in particular relates to a prodenia litura gene engineering virus used for carrying out deficiency and recombination on a wild type virus (SpltMNPV II) by utilizing a gene engineering method. The genome of the virus is deficient in an egt gene, namely an ecdysteroid UDP (uridine diphosphate) glucosyltransferase gene; and at the site of the deficient egt gene, a BmKITa1 gene controlled by a wild type virus, namely an early gene-1(ie-1) starter, and a marker gene, namely an enhanced green fluorescent protein (egfp) gene, which is controlled by the wild type virus, namely a polyhedron gene (ph) starterare inserted. The prodenia litura gene engineering virus No.3 disclosed by the invention has the advantages of faster insecticidal speed, less dosage effect and better field application effect compared with the wild type virus. Compared with the prior art, the prodenia litura gene engineering virus No.3 provided by the invention has less dosage effect and lower LT50 at low dose (105 polyhydral bodies/larva).

The present invention discloses a specific gene controlling growth of Arabidopsis vascular bundle and application thereof. The gene codes the protein of amino acid sequence shown in SEQ ID No.2 in sequence list and the genome gene is AT5g62940; the corresponding cNDA sequence is shown in the SEQ ID No.1 of the sequence list. As shown from research, the gene codes a transcription factor which is positioned in a core and has trans-activation activity, but the transcription factor which can regulate the formation of the Arabidopsis interfascicular cambium and the growth of vascular tissue is the transcription factor which is found as the first transcription factor for regulating the interfascicular cambium in Arabidopsis. In a transgenic gene plant, overexpression of the gene can thicken the stem of the plant, but inhibiting the expression of the gene in the plant can inhibit the occurrence of the interfascicular cambium. Therefore, the gene can be used for regulating the thickness of the stem, researching the woody plants with growth strength of secondary growth, and improving the quality. The gene has wide application prospect in regulating thickness growth and growth of the wood, flowers and plants.

The present invention provides a molecular mechanism for gene containment in sexually reproducing transgenic plants. The mechanism is achieved with a molecular construct comprising a blocking construct (BC) that is inserted fully or partially into an intron of a transgene of interest (TGI). The TGI encodes desired gene products, such as heterologous or homologous proteins, peptides or other useful products. The expression of the BC leads to block of at least one molecular or physiological function that is essential for development or reproduction of the transgenic plant. Thereby the BC expression leads to death or incapacity of sexual reproduction of the plant. Moreover, the mechanism comprises an externally applicable recovering tool to recover the functions blocked by the BC. The recovering tool may be a recovering construct (RC).

The invention discloses a quaternary ammonium type cationic starch gene controlled release vector material, and a preparation method and an application thereof; the preparation method particularly comprises the following steps: allowing starch which has a water content of 4.7-13.5% and a weight-average molecular weight of 13000-64000 Da to mix and react with a mixed system of solid caustic soda, water, and a quaternary ammonium type cationic etherifying agent which are stirred and well mixed through an ice water bath in a kneader by a dry method under a condition with a temperature of 25-30 DEG C and a rotating speed of 40-100 r/min for 12-48 hours; after the reaction is completed, washing, drying, and crushing to obtain the quaternary ammonium type cationic starch gene controlled releasevector material. An application of the vector material prepared by the invention as a non-viral vector material in gene therapy realizes the controlled release of gene drugs, improves the drug bioavailability, reduces the toxic and side effect of the drugs and the vector.

The present invention relates to a gene search vector, a random gene mutation control method and application thereof. Specifically, the present invention relates to a vector for gene search system, a random gene control method, a method for screening high transfer human tumor cell lines, a method for screening a gene for improving human tumor cell line transfer, a whole-genome random mutation library obtained by using the random gene control method, pharmaceutical application of the gene obtained by using the method for screening gene for improving human tumor cell line transfer, pharmaceutical application of an inhibitor of the gene, and pharmaceutical application of an inhibitor of an expression product of the gene. The gene obtained by the method of the invention, the inhibitor of the gene, the expression product of the gene and the inhibitor of the expression product of the gene can be applied to development of specific antitumor drug.

The invention relates to the technical field of maize gene engineering, in particular to application of a maize GRMZM2G417164 gene in adjustment of stomata development. The maize GRMZM2G417164, serving as a transcription factor, regulates some genes associated with the stomata development, and adjusts an associated process of maize stomata development. A series of experiments prove that a proteincoded by the GRMZM2G417164 gene controls transfer of a signal from a guard mother cell to a subsidiary mother cell and a final longitudinal division of the guard mother cell to form a guard cell. After the gene is mutant, a plant cannot form a normal guard cell and a normal subsidiary cell. Therefore, deep research and analysis on GRMZM2G417164 gene functions has an important theoretical significance and a practical application value for exploring a maize stomata development mechanism and improving maize resistance.

The invention discloses the function and the usage of a magnaporthe oryzae MoCHS1 gene and a coded protein thereof. The gene controls the conidium form, the conidium yield, the appressorium formation rate and the pathogenicity of magnaporthe oryzae. The gene as well as the cDNA and the coded protein thereof respectively comprise nucleotide or amino acid sequences SEQ ID No: 1, No: 2 and No: 3 in the sequence list. The knockout of the MoCHS1 gene causes the change of the form establishment of the magnaporthe oryzae conidium; the conidium is reduced and has no diaphragm or has only one diaphragm; the yield of the conidium is decreased to 2% of wild type bacterial strains; the formation rate of the appressorium is decreased to 60% of the wide type bacterial strains; and the infestation capability on rice vanes is obviously decreased. The protein coded by MoCHS1 or/and the expression or the modification of the homologous protein in other pathogenic fungi can be taken as important candidate targets and can be used for designing and screening novel antifungal drugs.