21 results about "Gene control" patented technology

The present invention encompasses compositions and methods to allow one to deliver and express multiple genes under the control of multiple gene regulatory components in a recipient cell via a synthetic chromosome. The engineering of synthetic chromosomes to contain multiple gene control units permits the construction of complex biological circuits.

The invention provides application of a product of targeted Vasorin protein in preparation of a product for diagnosing or treating kidney diseases with reduced glucose tolerance, and belongs to the technical field of biological medicines. Vasorin is used as a biomarker of the hypoglucose tolerance nephropathy, the level of Vasorin in urine of a patient is absolutely quantified through an ELISA technology, and the Vasorin has great clinical value and practical significance in early discovery and intervention of the hypoglucose tolerance nephropathy. According to the present invention, the gene or the Vasorin protein for controlling the Vasorin expression is adopted as the target spot, and the specific vector is adopted to perform overexpression on the Vasorin protein, such that the lipid droplet deposition and the reactive oxygen species (ROS) overgeneration in the renal tubular epithelial cell can be effectively improved, and the albumin reabsorption ability of the renal tubular can be improved so as to effectively treat the glucose tolerance reduced nephropathy.

Provided herein are KRN2 gene controlling kernel row number in plant, molecular markers closely linked to KRN2 and their application in molecular breeding.

This invention provides a gene related to the glossy leaf trait in Rugao black cabbage and its application, belonging to the field of molecular genetics and breeding technology. Specifically, this invention identifies the gene controlling the glossy leaf trait in 'Rugao black cabbage', confirms that this trait is controlled by a single dominant gene, and successfully locates the candidate gene. BraA02g02617P This study confirmed the association between the gene mutation and the glossy leaf trait. The discovery of the new dominant gene controlling glossy leaves greatly improves the convenience of molecular breeding of cruciferous plants, especially Brassica species, and has promising application prospects.

This invention relates to the field of biotechnology, specifically to the application of the ZmSKI3 and ZmSKI2 genes in regulating maize kernel development. This invention is the first to identify the ZmSKI3 and ZmSKI2 genes in maize and confirms that both genes control maize kernel development. Specifically, inhibiting the expression of ZmSKI3 and ZmSKI2 genes can delay maize kernel development. Therefore, this invention provides two key regulatory factors affecting maize kernel development, offering important molecular targets for improving maize kernel yield. Furthermore, this invention provides a novel gene resource for regulating maize kernel development; future manipulation of these genes can yield new germplasm with improved kernel development, enabling widespread application in production.

The present application discloses a kind of Magnaporthe grisea and Xanthomonas oryzae resistance related protein derived from rice and its coding gene and application.The present application provides a kind of method for improving the disease resistance of plant: the gene of plant coding OsAspRS protein is replaced by the gene of mutant protein.The present application also protects the application of osasprs mutant in the preparation of plant with improved disease resistance.osasprs mutant is a kind of spot-like mutant plant controlled by single recessive nuclear gene, which is screened by EMS on Huanghuazhan plant.Furthermore, the present application successfully locates and clones AspRS gene, and the mutation of the gene site can cause spot-like phenotype on rice leaf, and further improve the broad-spectrum disease resistance of rice.The present application is helpful to improve the disease resistance of crop, provides gene resources and technical support for cultivating new rice varieties with broad-spectrum resistance, and has important significance and application value for crop disease resistance breeding work.

The application discloses a ZmSKI3 gene related to corn disease resistance and application thereof, and belongs to the technical field of biotechnology.The ZmSKI3 gene is identified in corn for the first time, and it is proved that the ZmSKI3 gene controls the resistance of corn to cercospora leaf spot disease, specifically, the expression of the ZmSKI3 gene is inhibited, and the resistance of corn to the cercospora leaf spot disease can be enhanced; the overexpression of the ZmSKI3 gene can weaken the resistance of corn to the cercospora leaf spot disease.The mutation of the ZmSKI3 gene obtained by the application can significantly activate the disease resistance immune response of corn, is a new corn disease resistance breeding gene resource, and provides an important molecular target for the selection of resistant corn.

Various examples relate to a transgenic crop plant where a sequence of a cis-regulatory element in the promoter of certain genes is genomically changed in order to alter the flowering time or flowering rate of the plant. In one example, the cis-regulatory element in the promoter of such certain genes may be modified from TGAATG(A / T) (A / T / C) to TGAGGG(A / T) (A / T / C). The certain genes may be genes that encode SbEhd1, OsFTL10 / FT8, SbFT10, ZmELF3.2, or combinations thereof. And, the crop plant can be for example, sorghum, maize or rice. In another example, the present transgenic crop plant is a progeny plant. In yet another example, flowering time of the transgenic crop plant is regulated by a method that includes changing the sequence of the cis-regulatory element in the promoter of certain genes.

The application provides application of a preparation targeting a Vasorin protein in preparation of a diagnosis or treatment product for impaired glucose tolerance kidney disease, and belongs to the technical field of biological medicine. Vasorin is used as a biomarker for impaired glucose tolerance kidney disease, and absolute quantification of the level of Vasorin in urine of patients through ELISA technology has great clinical value and practical significance for early discovery and intervention of impaired glucose tolerance kidney disease. Taking a gene controlling expression of Vasorin or the Vasorin protein as a target, overexpression of the gene or the protein by using a specific carrier can effectively improve lipid droplet deposition in renal tubular epithelial cells, excessive generation of reactive oxygen species (ROS), and the ability of the renal tubular epithelial cells to reabsorb albumin, so that effective treatment of impaired glucose tolerance kidney disease is achieved.

The invention belongs to the technical field of plant genetic engineering, and relates to a rice HGR1 gene and application thereof in increasing rice grain weight. The invention discloses a rice HGR1 gene and a protein coded by the rice HGR1 gene, the genome nucleotide sequence of the HGR1 gene is shown as SEQ ID NO.1, the nucleotide sequence of a coding region of the HGR1 gene is shown as SEQ ID NO.2, and the sequence of the coded protein is shown as SEQ ID NO.3. The invention further discloses a preparation method of the rice HGR1 gene. The invention finds that the HGR1 gene controls the rice grain weight, knockout of the HGR1 gene enables the rice grain weight to be significantly increased, the grain length is increased, and grain filling is accelerated, which indicates that the HGR1 gene has potential utilization value in genetic improvement of the rice grain weight.

The present disclosure generally relates to engineered Cluster Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated (Cas) 12a proteins and system, and methods for use in gene editing and gene modulation for application to gene therapy. Related systems and methods of gene modulation are also disclosed.

This invention discloses a gene controlling spike length and grain number per spike in wheat. This gene is located on chromosome 2B using a recombinant inbred line population and high-density SNP markers, within the 657.82–664.40 Mb region of chromosome 2B in the wheat Chinese spring reference genome. A candidate gene, named TaeEF1A, was identified based on the wheat reference genome sequence, RNA-seq, and gene function. Gene editing of TaeEF1A further validated its function in regulating spike length, spikelet number, and grain number per spike. Through gene sequencing and analysis of the gene sequence in a large number of germplasm resources, key variations affecting spike length and grain number per spike were identified in the 3'-UTR of TaeEF1A. Based on this, a detection marker was designed for this gene, which can be used to detect superior allelic variants of the TaeEF1A gene. This marker can be easily, rapidly, and with high throughput applied to marker-assisted breeding to increase spike length, spikelet number, and grain number per spike, thereby increasing yield.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a rice GW7.2 gene and application thereof in regulation and control of rice grain weight and grain length. The invention discloses a rice GW7.2 gene and a protein coded by the rice GW7.2 gene, the genome nucleotide sequence of the GW7.2 gene is shown as SEQ ID NO.1, the nucleotide sequence of a coding region of the GW7.2 gene is shown as SEQ ID NO.2, and the amino acid sequence of the coded protein is shown as SEQ ID NO.3. The invention further discloses a preparation method of the rice GW7.2 gene. The invention provides a technology for increasing the grain weight and the grain length of rice by using a rice gene GW7.2, and finds that the GW7.2 gene controls the grain weight and the grain length of the rice, and knockout of the GW7.2 gene enables the grain weight of the rice to be remarkably increased and the grain length to be remarkably increased, so that the GW7.2 gene has potential utilization value in genetic improvement and molecular breeding of the grain weight and the grain shape of the rice.

The invention discloses a gene for controlling plant branching and application thereof. According to the invention, a new gene OsDIP8 for regulating and controlling the growth and development of rice tillering is screened and cloned in rice for the first time, and after the gene is over-expressed, the number of rice tillering is remarkably increased, which proves that the gene has a positive regulation and control effect on the important agronomic trait, namely the number of rice tillering. According to the invention, the important regulation effect of the molecular chaperonin on the important agronomic trait tillering number of rice is identified for the first time, and new genes and germplasm resources are provided for deep research of a plant branching or tillering regulation mechanism and rice plant type breeding.

The application discloses a ctPistol ribozyme and application thereof in gene silencing, and belongs to the technical field of biology.The disclosed ctPistol ribozyme can realize effective control of cell phenotype by silencing the expression of key genes.In addition, when applied in a mouse cancer model, the ctPistol realizes a powerful and persistent anti-tumor effect by targeting oncogenic driver genes.As a powerful gene silencing tool and a promising RNA-based therapeutic candidate drug, the ctPistol ribozyme shows an effective way of developing diversified ribozyme-mediated gene control technology.

To provide function-deficient transcription factor-like genes and inbreeding Fagopyrum plants using the same.SOLUTION: The function of transcription factor-like genes formed by a group of linked genes governing the length of the male core and the female core of a Fagopyrum plant identified from the result of decoding the genome sequence of the Fagopyrum plant is deleted by mutation induction to produce S-ELF3-PS1 genes which are function-deficient transcription factor-like genes, and an individual having the S-ELF3-PS1 inbreeding genes is cross-cultivated to grow a inbreeding Fagopyrum plant having long male core and female core.SELECTED DRAWING: Figure 1

This application relates to the field of molecular markers, specifically to molecular markers related to the easy separability of mature fruit and pedicel in chili peppers, their applications, and detection methods. This invention locates the gene controlling the easy separability of mature fruit and pedicel to chromosome 10, obtaining candidate regions for this gene. Molecular markers closely linked to this trait are then developed within these candidate regions. The invented molecular markers are closely linked to the gene controlling the easy separability of mature fruit and pedicel in chili peppers, exhibiting high identification efficiency. The development and utilization of these markers allows for the identification of whether the fruit and pedicel are easily separable even at the seedling stage.

The invention discloses application of soybean GmCDC7 protein and a coding gene thereof in regulation and control of soybean quality traits. The GmCDC7 gene encodes a plant germination-like protein, and the amino acid sequence of the GmCDC7 gene is as shown in SEQ ID No.2. According to the invention, gene editing is carried out on the GmCDC7 gene of a receptor plant Jack by using a CRISPR-Cas9 technology, and a homozygous mutant of the GmCDC7 gene is obtained. Compared with a wild type, the soybean seed protein content of a GmCDC7 gene mutant plant is remarkably increased, and the oil content is remarkably reduced. The result shows that the GmCDC7 gene negatively regulates and controls the protein content of the soybean seeds and influences the oil content of the seeds at the same time. The invention discloses the biological function of the soybean GmCDC7 gene for controlling the quality traits of soybean seeds for the first time.

This application discloses a novel algorithm for constructing a genetic map of a MAGIC population, comprising: collecting species data; segmenting chromosomes based on the collected data to form chromosome fragments; calculating the parental genetic similarity index of chromosome fragments to determine parental origin; obtaining a definite origin matrix and a fuzzy origin matrix; using definite fragments from a single parental origin in the definite origin matrix as anchor points; using the anchor points as starting points, examining adjacent fragments of fuzzy fragments in the fuzzy origin matrix to determine parental origin; merging fragments with the same parental origin and adjacent positions into a larger fragment to form a parental origin matrix; calculating recombination rate and genetic distance; constructing a genetic map; and using the novel algorithm to analyze the whole-genome resequencing data of a MAGIC population of a specified crop, thereby effectively detecting minor genes controlling complex quantitative traits, clarifying the parental origin of each variation site, and finding the required high-quality parents, thereby improving the breeding process.

This invention relates to the field of molecular markers for peppers, and discloses an Indel molecular marker for identifying genes controlling light-induced purple color in pepper pericarps and its application. The applicant used pooled transcriptome sequencing (BSR-seq) combined with linkage mapping to identify the relevant sites controlling light-induced purple color in pepper pericarps, and designed the marker A_836 and detection primers based on the inserted Indel. By detecting the deletion or insertion of the provided specific fragment, the fruit color of peppers can be accurately and rapidly detected at the seedling stage, eliminating the need to wait until the fruit setting stage. This reduces labor, significantly improves breeding efficiency, and lowers breeding costs.

The invention relates to DNA of a nitrogen-efficient gene OsAPTN1 for regulating tillering and yield of rice and breeding utilization of protein of the nitrogen-efficient gene OsAPTN1. A knockout and overexpression material of the OsAPTN1 gene is obtained through a gene editing technology and the gene. Overexpression of the OsAPTN1 improves tillering and yield of the rice; the tillering and yield of the rice are reduced by knocking out the OsAPTN1; the SNP and indel of the OsAPTN1 coding region cause the change of the OsAPTN1 protein structure, and the OsAPTN1 protein may have different transport capabilities. The OsAPTN1 excellent haplotype promotes the growth of effective tillering of rice and improves the nitrogen utilization efficiency and yield. The OsAPTN1 gene and the excellent haplotype genetic material thereof provide gene resources, materials and technical support for cultivating green nitrogen efficient rice varieties.