Prodenia litura gene engineering virus No.3 and building method thereof

A technology of genetic engineering and Spodoptera litura, applied in the field of microorganisms, to achieve the effect of good field application effect, low dose effect and fast insecticidal speed

Inactive Publication Date: 2012-01-25
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there has been no successful construction of the Spodoptera litura nuclear polyh

Method used

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  • Prodenia litura gene engineering virus No.3 and building method thereof
  • Prodenia litura gene engineering virus No.3 and building method thereof
  • Prodenia litura gene engineering virus No.3 and building method thereof

Examples

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Embodiment 1

[0024] See attached figure 1 , it is a kind of Spodoptera litura genetic engineering virus provided by the present invention, namely the structural representation of Spodoptera litura genetic engineering virus No. 3; Among the figure, egt : ecdysone uridine diphosphate glucosyltransferase gene; P pH : polyhedrin gene promoter; P ie- 1: early gene promoter; SignalP : SpltMNPV F protein signal peptide sequence; BmK IT 1: East Asian pincer scorpion venom gene; egfp : Enhanced green fluorescent protein gene. The marker gene of the recombinant virus is enhanced green fluorescent protein EGFP, which can be monitored and identified by the green fluorescence produced by the green fluorescent protein during the purification and propagation of engineered virus No. 3.

[0025] See attached figure 2 , which is a schematic diagram of the construction process of the Spodoptera litura genetically engineered virus No. 3 provided by the present embodiment. In the figure, egt i...

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PUM

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Abstract

The invention discloses a prodenia litura gene engineering virus No.3 and a building method thereof, in particular relates to a prodenia litura gene engineering virus used for carrying out deficiency and recombination on a wild type virus (SpltMNPV II) by utilizing a gene engineering method. The genome of the virus is deficient in an egt gene, namely an ecdysteroid UDP (uridine diphosphate) glucosyltransferase gene; and at the site of the deficient egt gene, a BmKITa1 gene controlled by a wild type virus, namely an early gene-1(ie-1) starter, and a marker gene, namely an enhanced green fluorescent protein (egfp) gene, which is controlled by the wild type virus, namely a polyhedron gene (ph) starterare inserted. The prodenia litura gene engineering virus No.3 disclosed by the invention has the advantages of faster insecticidal speed, less dosage effect and better field application effect compared with the wild type virus. Compared with the prior art, the prodenia litura gene engineering virus No.3 provided by the invention has less dosage effect and lower LT50 at low dose (105 polyhydral bodies/larva).

Description

technical field [0001] The invention relates to a Spodoptera litura genetic engineering virus capable of preventing and controlling agricultural and forestry pests such as Spodoptera litura, belonging to the technical field of microorganisms. Background technique [0002] Spodoptera litura is one of the main pests of crops, and it is distributed in all provinces and autonomous regions of my country. The insectivorous nature is extremely wide, and the host plants known to be harmful are more than 290 kinds in 99 families. In recent years, with the change of agricultural farming system and the rapid development of urbanization, the planting area of ​​dryland crops such as vegetables, flowers, and green lawns has increased, and the damage of Spodoptera litura has become increasingly rampant. The chemical control currently used in production often fails to achieve the desired effect, and is likely to cause environmental pollution, pesticide residues, and human and animal poison...

Claims

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Application Information

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IPC IPC(8): C12N7/01C12N15/63C12R1/93
Inventor 朱江浦冠勤郭大磊王文兵汤欣欣孙兴鲁秦启联
Owner SUZHOU UNIV
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