175 results about "Order Lepidoptera" patented technology

The invention relates to the technical field of microorganisms, in particular to serratia marcescens and application of the serratia marcescens in prevention and treatment of lepidoptera pests. The serratia marcescens CB08 provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number of the serratia marcescens CB08 is CCTCC (China Center for Type Culture Collection) NO: M 2025266. The strain has a remarkable prevention and control effect on Lepidoptera pests such as spodoptera frugiperda, armyworm, prodenia litura and cotton bollworm, has no pathogenicity on host crops such as corn, is a bacterium with excellent Lepidoptera pest prevention and control potential, has a very wide application prospect, and is expected to bring a new solution to the field of agricultural pest prevention and control.

The invention mainly provides Beauveria bassiana ZABb200102 and application of the Beauveria bassiana ZABb200102, the Beauveria bassiana ZABb200102 is preserved in China General Microbiological Culture Collection Center on October 28, 2024, and the preservation number of the Beauveria bassiana ZABb200102 is CGMCC (China General Microbiological Culture Collection Center) No. 41557. The strain is separated from sick lepidoptera cacalidae larva bodies for the first time, and it is found through toxicity determination that the strain has high pathogenicity to tomato leaf miner larvae. In addition, the strain can also be colonized in tomatoes, so that the growth of the tomatoes is promoted, and the resistance of the tomatoes to pests is indirectly improved. Therefore, the strain can be widely applied to development of tomato bacterial fertilizer and green prevention and control of tomato leaf miner which is an important tomato pest.

The invention discloses an RNA (Ribonucleic Acid) interference method for parasitic wasps in lepidoptera larvae, which is characterized in that RNA interference is carried out by using a microinjection method or a nano-material immersion method when the parasitic wasps in the lepidoptera larvae are not separated from hosts or are separated from the hosts to cocoon, so that an RNAi system of the parasitic wasps is successfully constructed, and the defects that the parasitic wasps are small in size in an egg stage, and the parasitic wasps are not damaged in the egg stage can be effectively overcome. And RNAi operation difficulty caused by cocoon wrapping in the pupal stage and short life in the adult stage is avoided. Moreover, the method disclosed by the invention is simple to operate and high in interference efficiency, provides a theoretical basis and a reference effect for further researching gene functions of the parasitic wasps, and has important significance on biological control.

The invention discloses an insect-resistant transgenic cotton event BrsC35 as well as a detection method and application thereof. According to the invention, an epsps gene, a Cry1Ac gene and a Vip3A gene are transferred into a genome of Zhongmiansui 24 through an agrobacterium-mediated method, so that a transgenic cotton event BrsC35 of which the resistance to lepidoptera insects such as cotton bollworms, ostrinia nubilalis and spodoptera frugiperda is remarkably improved is obtained. The transgenic cotton event BrsC35 is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 17, 2025, and the preservation number is CGMCC No.32181. The transgenic cotton event BrsC35 has been preserved in China General Microbiological Culture Collection Center (CGMCC). The invention also provides a method for identifying whether a plant sample is derived from the transgenic cotton event BrsC35 or an offspring of the transgenic cotton event BrsC35, the transgenic cotton event BrsC35 can be specifically detected, and the transgenic cotton event BrsC35 can be better supervised and managed. The method has an important application value.

The present invention relates to insect neuropeptide compounds of the family of pyroglutamic peptides and analogs thereof having activity against insects, such as Hemiptera, Diptera, Lepidoptera, Blattaria and / or Coleoptera insects, such as aphids and fruit flies, and their use as insect control agents (e.g. insecticides) and plant protection agents.

The invention discloses a scotogramma trifolii specific SS-COI primer pair and a rapid molecular detection method, an amplified fragment of the primer pair is a specific region of 227bp in a scotogramma trifolii COI gene, rapid detection and identification of scotogramma trifolii in lepidoptera insects can be realized by using the primer pair, sequencing and sequence alignment are not needed, and the primer pair can be used for rapidly detecting and identifying the scotogramma trifolii in the lepidoptera insects. The kit has the advantages of high sensitivity, strong specificity, simplicity and convenience in operation, good repeatability and the like.

The invention provides a lepidoptera pest egg mass lossless collection method and a portable device based on vibration separation, and relates to the technical field of pest egg mass collection, the portable device comprises a bottom plate, a supporting rod, a side plate and a fixing piece, a sliding mechanism is arranged at the rear end of the bottom plate, a rotating mechanism is arranged in the side plate, and the sliding mechanism comprises an entering cavity, an arc-shaped hopper, an arc-shaped partition plate, a storage cavity, a screen and the like. The rotating mechanism comprises a metal sliding block, a rotating shaft, a connecting piece, a support, an annular magnet and the like, and is further provided with a rotating disc, a spring, a brush and the like. By means of the structure, after soil blocks with eggs are placed into the entering cavity and separated through the arc-shaped partition plate, the rotating disc is rotated to enable the rotating shaft to drive the support to rotate, the metal sliding block moves up and down to generate vibration, the soil blocks are stripped through the adjustable brush, and the egg blocks fall into the containing cavity through the screen; the lepidoptera pest egg masses in soil blocks with different viscosities can be conveniently, efficiently and nondestructively separated and collected, and the device is suitable for the field environment.

The present invention relates to analogues of insect neuropeptides that are active against insects, such as Diptera, Hemiptera, Blattodea and / or Lepidoptera, and to the use of these as insect control agents (e.g., insecticides) and plant protection agents.

The present invention relates to an insecticidal composition comprising of N-(phenylthio) phthalimide (NPP), neem oil and chitosan and method of preparation thereof. The composition of the present invention is useful against insects selected from Helicoverpa armigera, Mythimna separata, Spodoptera frugiperda and other Lepidopteran species of insects. The composition can serve as a formulation that possesses a lower probability of acquiring resistance.

The invention belongs to the technical field of organic matter preparation, and provides an isoxazoline compound containing benzamide as well as a preparation method and application thereof.Chemical modification and molecular design are performed on a compound with an isoxazoline structure, a substituent group is introduced to N of an amide group, and the benzamide-containing isoxazoline compound is prepared. A series of high-efficiency compounds which can be used for killing insects or mites in agriculture or forestry and have excellent activity are obtained, and particularly, the compounds have very good biological activity on pests of lepidoptera, hemiptera and the like. When being used at an extremely low concentration, the compound disclosed by the invention also shows an excellent control effect on various pests or pest mites, has insecticidal or acaricidal activity in each stage (such as eggs, larvae (nymphs), pupae and adults) of the life cycle of the pests or pest mites, and has an excellent control effect on the pests or pest mites which have drug resistance to traditional insecticides or acaricides. And the composition also shows excellent control activity.

The application discloses application of a microbial natural product GameXPeptide-A in improving the control effect of insecticides on pests, and belongs to the technical field of biological control. Experiments prove that GameXPeptide-A can effectively improve the insecticidal activity of insecticides on agricultural pests such as thrips, corn borer and prodenia litura when GameXPeptide-A is combined with insecticides such as Metarrhizium anisopliae, Beauveria bassiana, nuclear polyhedrosis virus, 4% abamectin and pyridaben, 10% dimethoate and spinosad, 30% ethoxazole, 45% bifenthrin and etoxazole, 5% spinosad, 5% chlorantraniliprole, 5% abamectin and the like, and it is indicated that the substance can be combined with different insecticides to improve the insecticidal activity of insecticides on pests such as Lepidoptera, Diptera, Coleoptera, Orthoptera, Hemiptera and Homoptera in agricultural production, and can be used for the development and application of a new type of pest immunosuppressant or insecticide synergist.

The present disclosure provides compositions nanocomposite biopesticides exhibiting improved efficacy, photostability, as well as controlled release and systemic delivery characteristics and methods of making and using the same. The present disclosure further provides methods for protecting a plant from a Lepidopteran pest as well as controlling a Lepidopteran pest.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

This invention discloses a natural plant-extracted biological insecticide and its preparation and application methods, belonging to the field of agricultural biotechnology. The insecticide uses cypress leaves, stemona root, clematis root, pinellia root, and ailanthus leaves as raw materials, prepared through boiling, cooling, filtration, sealed maturation, double-barrel water bath temperature gradient concentration, and pasteurization. This insecticide kills pests by sealing their spiracles and rapidly penetrating their bodies, causing suffocation. It primarily works through contact, exhibiting good residual effect, low resistance development, no chemical residues, and is environmentally friendly. The preparation process is mild, maximizing the retention of heat-sensitive active ingredients. The product is highly stable and low-cost, particularly suitable for controlling Diptera, Coleoptera, Orthoptera, Hemiptera, and / or Lepidoptera pests.

The invention belongs to the field of biological control, and relates to application and a method of dsRNA of an NRX gene in control of lepidoptera pests. It is found for the first time that the Neurexin-4 gene participates in the growth and development process of the cotton bollworm and the spodoptera frugiperda, the death rate of larvae of the cotton bollworm and the spodoptera frugiperda is remarkably increased through deletion of Neurexin-4 expression, and finally population development is inhibited. The gene can be used for developing RNA biopesticides for preventing and controlling cotton bollworms and spodoptera frugiperda. The invention provides an interference sequence for the cotton bollworm and grassland spodoptera Neurexin-4 gene, the sequence can inhibit the expression of Neurexin-4, and the sequence can be utilized to interfere the expression of the cotton bollworm and grassland spodoptera Neurexin-4 gene and inhibit the growth of the cotton bollworm and grassland spodoptera Neurexin-4 gene.

The present invention relates to a method o f combating and / or controlling a pest selected from the Lepidoptera order, which comprises applying to said pest, to a locus of said pest, to a plant susceptible to attack by said pest, or to a plant propagation material susceptible to attack by said pest, a pesticidal composition comprising isocycloseram as active ingredient A, and cartap as active ingredient B.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The invention discloses a lepidoptera pest catching device, and belongs to the technical field of pest control. The device is mainly composed of a base, a rectangular base frame, a rectangular frame, a trap lamp, a top box, a strip-shaped overturning piece, an overturning mechanism, an insecticide storage box and related auxiliary assemblies. The area of the rectangular frame is large so that the insect catching amount can be increased. After pests enter, the overturning mechanism drives the narrow strip-shaped overturning piece to rapidly and synchronously rotate, the frame is closed, meanwhile, the sliding plate synchronously seals the discharging hopper, the response time of the pests is greatly shortened, and the pests are effectively prevented from escaping. After closing, the insecticide is treated by the atomizer and uniformly fills the frame under the action of the fan and the guide plate, and the high-concentration insecticide can thoroughly kill insects and reduce the dosage. Insect corpses fall into the discharging hopper through the through holes in the bottom of the rectangular frame and are finally discharged through the guide-out plate. The device has the advantages of being high in insect catching efficiency, thorough in insect killing, capable of saving energy, environmentally friendly and the like, and is suitable for lepidoptera pest control work in various scenes such as farmlands, orchards and flower beds.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

The invention discloses application of an RAD9A gene as a target spot in preparation of a preparation for preventing and treating insects. The RAD9A gene of the plutella xylostella is knocked out by utilizing a CRISPR-Cas9 technology, and four kinds of homozygous mutants are obtained through screening. Wherein most of homozygous mutant embryos are lethal, and cavities of surviving homozygous mutant larva testis are increased and fused, and structural deformity such as whole atrophy of the testis occurs. Development of progenies of the surviving homozygous mutants is delayed, and the progenies finally die According to the invention, a UAS-GAL4 system is utilized to construct a fruit fly strain of specific RNAi RAD9A in a testis. Reduction of RAD9A expression can obviously cause apoptosis of drosophila larva testis cells, which indicates that RAD9 has a conservative function in insect testis development. Based on the ORF sequence conservative property of the insect RAD9A, the mutation of the region can be used as a control target of various pests. Based on the CRISPR-Cas9 technology, the RAD9A knockout target spot designed in the invention can play a similar effect on the lepidoptera insects.

The utility model belongs to the technical field of lepidoptera insect breeding, and particularly relates to an automatic pupa collecting lepidoptera insect breeding box which comprises a breeding box body, a breeding box cover, a pupa collecting cluster and an excrement collecting groove. The feeding box is provided with the insertable pupa collecting cluster, the habit that lepidoptera insects search for cracks to spin silks, cocoon and pupate is utilized, mature larvae are attracted to be mounted on the cluster and pupae, pupa collecting efficiency is greatly improved, and the situation that pupa bodies are damaged and eclosion cannot be achieved due to manual pupa picking is avoided; the excrement collecting groove is formed in the bottom of the feeding box, so that insect excrement in the box can be easily collected, the excrement cleaning working efficiency in the insect feeding process is greatly improved, and the pathogenic bacterium infection condition caused by contact between accumulated insect excrement and insect bodies is avoided; the breeding box is provided with a temperature and humidity sensor and an air supply system, once it is detected that temperature and humidity deviate from a set range, a cold air or warm air or humidifier can be triggered to be started, and meanwhile the situation in the box can be monitored in real time.

The present invention relates to a method of controlling or preventing infestation of a plant by lepidoptera or coleopteran insects, comprising applying a combination or composition to the plant, its locus or its propagation material in any desired sequence or simultaneously, the combination or composition comprises as component (A) a compound of formula (A-1) and as component (B) a compound of formula (B-1). Compositions suitable for use in the invention are also disclosed. (A-1) is greater than (B-1).

The present invention relates to a method for controlling hemipteran or lepidopteran insect pests in crops, said method comprising applying to a plant, a plant propagation material, locus or parts thereof or surrounding soil with the composition comprising a mixture of elemental Sulphur in a concentration range of 1% to 90% by weight of the total composition; Selenium in its elemental form or salts or complexes or derivatives or mixture thereof wherein elemental Selenium content is in the range of 0.001% to 10% by weight of the total composition; and at least one agriculturally acceptable excipients wherein the composition comprises particles in the size range of 0.1 to 50 microns.

Chiral isomeric sulfite compounds, and preparative separation and application of chiral structures thereof are disclosed, which belong to the technical field of agriculture. The present disclosure has found that the chiral isomeric sulfite compounds having the structure of general formula (A) have excellent inhibitory activities against pest eggs, especially have strong inhibitory activities against eggs of thrips, mites, Lepidoptera moths, Aleyrodidae and Harmonia axyridis, and meanwhile have germicidal activity, and their absolute configuration are obtained through preparative separation. The compounds with such structures can be used for killing pest eggs and germs, and have high research value of agrochemical, having broad application prospects in the field of agricultural pharmacy.

Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, TIC11239, TIC11243, TIC11256, TIC4544, TIC4545, TIC6871, TIC7429, TIC7497, TIC7511, TIC7513, TIC7518, TIC7524, TIC7526, TIC7528, TIC7535 and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.

An insecticidal protein, a nucleic acid molecule encoding the protein, and a method and the use thereof for controlling Lepidopteran pests. The insecticidal protein contains an amino acid sequence as set forth in SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 11 or SEQ ID NO: 33. The modified Cry1Da1 protein has a better insecticidal effect, with significantly greater insecticidal activity compared to the patented protein BD1-002.

The present invention relates to natural or natural-like analogues of insect pyrokinin neuropeptide [Pyr]-AIMARPQVPRL-[NH2](SEQ ID NO:2) having activity against insects, for example hemipteran, dipteran, lepidopteran, blattodean and / or coleopteran insects, such as aphids, moths and fruit flies, and their use as insect control agents (e.g. insecticides) and plant protection agents.

Provided are a nucleic acid sequence for detecting a Glycine max plant DBN8205 and a detection method therefor. The nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and / or SEQ ID NO: 2 or a complementary sequence thereof. The Glycine max plant DBN8205 has good resistance to insects of Lepidoptera order and good tolerance to glufosinate herbicides, and therefore the yield is not affected. By utilizing the detection method, it is possible to accurately and quickly identify whether a biological sample contains a DNA molecule of a transgenic Glycine max event DBN8205.

Pesticidal proteins exhibiting inhibitory, suppressive, and toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC4064 and TIC4064 amino acid sequence variants. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC4064 and TIC4064 amino acid sequence variant pesticidal proteins are also provided.

To provide a method for producing a sterile lepidopteran insect by a sterilization method which can simply, stably and efficiently sterilize a lepidopteran insect and causes abnormality only in germ line cells, and a sterilizing composition capable of executing the method.SOLUTION: The expression of nanosO and nanosP genes in a host lepidopteran insect is suppressed.SELECTED DRAWING: None