103 results about "Order Lepidoptera" patented technology

The present invention relates to insect neuropeptide compounds of the family of pyroglutamic peptides and analogs thereof having activity against insects, such as Hemiptera, Diptera, Lepidoptera, Blattaria and / or Coleoptera insects, such as aphids and fruit flies, and their use as insect control agents (e.g. insecticides) and plant protection agents.

The present invention relates to analogues of insect neuropeptides that are active against insects, such as Diptera, Hemiptera, Blattodea and / or Lepidoptera, and to the use of these as insect control agents (e.g., insecticides) and plant protection agents.

The present invention relates to an insecticidal composition comprising of N-(phenylthio) phthalimide (NPP), neem oil and chitosan and method of preparation thereof. The composition of the present invention is useful against insects selected from Helicoverpa armigera, Mythimna separata, Spodoptera frugiperda and other Lepidopteran species of insects. The composition can serve as a formulation that possesses a lower probability of acquiring resistance.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

This invention discloses a natural plant-extracted biological insecticide and its preparation and application methods, belonging to the field of agricultural biotechnology. The insecticide uses cypress leaves, stemona root, clematis root, pinellia root, and ailanthus leaves as raw materials, prepared through boiling, cooling, filtration, sealed maturation, double-barrel water bath temperature gradient concentration, and pasteurization. This insecticide kills pests by sealing their spiracles and rapidly penetrating their bodies, causing suffocation. It primarily works through contact, exhibiting good residual effect, low resistance development, no chemical residues, and is environmentally friendly. The preparation process is mild, maximizing the retention of heat-sensitive active ingredients. The product is highly stable and low-cost, particularly suitable for controlling Diptera, Coleoptera, Orthoptera, Hemiptera, and / or Lepidoptera pests.

The invention belongs to the field of biological control, and relates to application and a method of dsRNA of an NRX gene in control of lepidoptera pests. It is found for the first time that the Neurexin-4 gene participates in the growth and development process of the cotton bollworm and the spodoptera frugiperda, the death rate of larvae of the cotton bollworm and the spodoptera frugiperda is remarkably increased through deletion of Neurexin-4 expression, and finally population development is inhibited. The gene can be used for developing RNA biopesticides for preventing and controlling cotton bollworms and spodoptera frugiperda. The invention provides an interference sequence for the cotton bollworm and grassland spodoptera Neurexin-4 gene, the sequence can inhibit the expression of Neurexin-4, and the sequence can be utilized to interfere the expression of the cotton bollworm and grassland spodoptera Neurexin-4 gene and inhibit the growth of the cotton bollworm and grassland spodoptera Neurexin-4 gene.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The invention discloses a lepidoptera pest catching device, and belongs to the technical field of pest control. The device is mainly composed of a base, a rectangular base frame, a rectangular frame, a trap lamp, a top box, a strip-shaped overturning piece, an overturning mechanism, an insecticide storage box and related auxiliary assemblies. The area of the rectangular frame is large so that the insect catching amount can be increased. After pests enter, the overturning mechanism drives the narrow strip-shaped overturning piece to rapidly and synchronously rotate, the frame is closed, meanwhile, the sliding plate synchronously seals the discharging hopper, the response time of the pests is greatly shortened, and the pests are effectively prevented from escaping. After closing, the insecticide is treated by the atomizer and uniformly fills the frame under the action of the fan and the guide plate, and the high-concentration insecticide can thoroughly kill insects and reduce the dosage. Insect corpses fall into the discharging hopper through the through holes in the bottom of the rectangular frame and are finally discharged through the guide-out plate. The device has the advantages of being high in insect catching efficiency, thorough in insect killing, capable of saving energy, environmentally friendly and the like, and is suitable for lepidoptera pest control work in various scenes such as farmlands, orchards and flower beds.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

The invention discloses application of an RAD9A gene as a target spot in preparation of a preparation for preventing and treating insects. The RAD9A gene of the plutella xylostella is knocked out by utilizing a CRISPR-Cas9 technology, and four kinds of homozygous mutants are obtained through screening. Wherein most of homozygous mutant embryos are lethal, and cavities of surviving homozygous mutant larva testis are increased and fused, and structural deformity such as whole atrophy of the testis occurs. Development of progenies of the surviving homozygous mutants is delayed, and the progenies finally die According to the invention, a UAS-GAL4 system is utilized to construct a fruit fly strain of specific RNAi RAD9A in a testis. Reduction of RAD9A expression can obviously cause apoptosis of drosophila larva testis cells, which indicates that RAD9 has a conservative function in insect testis development. Based on the ORF sequence conservative property of the insect RAD9A, the mutation of the region can be used as a control target of various pests. Based on the CRISPR-Cas9 technology, the RAD9A knockout target spot designed in the invention can play a similar effect on the lepidoptera insects.

The utility model belongs to the technical field of lepidoptera insect breeding, and particularly relates to an automatic pupa collecting lepidoptera insect breeding box which comprises a breeding box body, a breeding box cover, a pupa collecting cluster and an excrement collecting groove. The feeding box is provided with the insertable pupa collecting cluster, the habit that lepidoptera insects search for cracks to spin silks, cocoon and pupate is utilized, mature larvae are attracted to be mounted on the cluster and pupae, pupa collecting efficiency is greatly improved, and the situation that pupa bodies are damaged and eclosion cannot be achieved due to manual pupa picking is avoided; the excrement collecting groove is formed in the bottom of the feeding box, so that insect excrement in the box can be easily collected, the excrement cleaning working efficiency in the insect feeding process is greatly improved, and the pathogenic bacterium infection condition caused by contact between accumulated insect excrement and insect bodies is avoided; the breeding box is provided with a temperature and humidity sensor and an air supply system, once it is detected that temperature and humidity deviate from a set range, a cold air or warm air or humidifier can be triggered to be started, and meanwhile the situation in the box can be monitored in real time.

The present invention relates to a method of controlling or preventing infestation of a plant by lepidoptera or coleopteran insects, comprising applying a combination or composition to the plant, its locus or its propagation material in any desired sequence or simultaneously, the combination or composition comprises as component (A) a compound of formula (A-1) and as component (B) a compound of formula (B-1). Compositions suitable for use in the invention are also disclosed. (A-1) is greater than (B-1).

Chiral isomeric sulfite compounds, and preparative separation and application of chiral structures thereof are disclosed, which belong to the technical field of agriculture. The present disclosure has found that the chiral isomeric sulfite compounds having the structure of general formula (A) have excellent inhibitory activities against pest eggs, especially have strong inhibitory activities against eggs of thrips, mites, Lepidoptera moths, Aleyrodidae and Harmonia axyridis, and meanwhile have germicidal activity, and their absolute configuration are obtained through preparative separation. The compounds with such structures can be used for killing pest eggs and germs, and have high research value of agrochemical, having broad application prospects in the field of agricultural pharmacy.

Insecticidal proteins exhibiting toxic activity against Coleopteran and Lepidopteran pest species are disclosed, and include, but are not limited to, TIC3668, TIC3669, TIC3670, TIC4076, TIC4078, TIC4260, TIC4346, TIC4826, TIC4861, TIC4862, TIC4863, TIC11239, TIC11243, TIC11256, TIC4544, TIC4545, TIC6871, TIC7429, TIC7497, TIC7511, TIC7513, TIC7518, TIC7524, TIC7526, TIC7528, TIC7535 and TIC-3668-type proteins. DNA molecules and constructs are provided which contain a polynucleotide sequence encoding one or more of the disclosed TIC3668-type proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran and Coleopteran infestation are provided which contain polynucleotide sequences encoding the insecticidal proteins of the present invention. Methods for detecting the presence of the polynucleotides or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using any of the TIC3668-type insecticidal proteins are also provided.

Provided are a nucleic acid sequence for detecting a Glycine max plant DBN8205 and a detection method therefor. The nucleic acid sequence comprises SEQ ID NO: 1 or a complementary sequence thereof, and / or SEQ ID NO: 2 or a complementary sequence thereof. The Glycine max plant DBN8205 has good resistance to insects of Lepidoptera order and good tolerance to glufosinate herbicides, and therefore the yield is not affected. By utilizing the detection method, it is possible to accurately and quickly identify whether a biological sample contains a DNA molecule of a transgenic Glycine max event DBN8205.

Pesticidal proteins exhibiting inhibitory, suppressive, and toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC4064 and TIC4064 amino acid sequence variants. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC4064 and TIC4064 amino acid sequence variant pesticidal proteins are also provided.

This invention belongs to the field of pesticide compound technology, specifically relating to an insecticidal composition. The active ingredients of the insecticidal composition include active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (Ⅰ), and active ingredient B is an arylisoxazoline insecticide. The mass ratio of active ingredient A to active ingredient B is 1:30 to 40:1. The compound insecticide of this invention has a significant synergistic effect, reduces the amount of insecticide used, lowers production costs and environmental risks, delays the development of insecticide resistance in pests, and can be used for integrated management of resistant pests, specifically for controlling Hemiptera, Lepidoptera, and Coleoptera pests.

The present application belongs to the technical field of microorganisms, and discloses a new Talaromyces amestolkiae strain with broad-spectrum lepidopterous insecticidal activity and application thereof. The new strain is isolated from the pests of Spodoptera litura, and is named as Talaromyces amestolkiae GZTA01, which has been preserved in China Center for Type Culture Collection on January 6, 2026, and the strain preservation number is CCTCC NO: M2026033. The Talaromyces amestolkiae strain of the present application has a minimum lethal rate of 62.14% and a maximum lethal rate of 86.67% on Spodoptera litura, Myzus persicae, Plutella xylostella and Mythimna separata, and exhibits excellent broad-spectrum control potential on lepidopterous and hemipterous pests.

Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

The application relates to a chimeric insecticidal protein and its use, the protein comprising domain I of a CrylAc protein, domain II and domain III of a Cryllgg protein which are sequentially connected, and the chimeric insecticidal protein has strong body weight inhibiting activity and lethal activity on multiple lepidopteran pests. The provided chimeric insecticidal protein not only widens the insect resistance spectrum of the original protein, but also enhances the toxicity to pests, can effectively slow down the occurrence of pest resistance in nature, and has a good application prospect in agricultural production.

This utility model discloses an artificial carrier conservation device for Trichogramma wasps in the field, relating to the field release and application technology of Trichogramma wasps. It includes: a base, a cage, a trapping unit, an oviposition medium, and a curtain. The cage is fixedly connected to the base, with mesh openings on its side walls and a top opening. The trapping unit includes a light-transmitting funnel and an insect-attracting lamp. The insect-attracting lamp is located inside the cage, and the light-transmitting funnel is connected to the top opening of the cage and extends into the cage to form a one-way channel for attracting lepidopteran insects into the cage. Multiple hanging positions are provided inside the cage, and the oviposition medium is detachably suspended at these positions so that lepidopteran insects entering the cage can lay their eggs on the medium. The curtain is installed on the side walls of the cage and can be opened or closed to allow light to be blocked from entering the cage. This utility model has a simple structure and is easy to use, creating suitable living and reproductive conditions for Trichogramma wasps and achieving long-term effective control of lepidopteran pests in the field.

This invention discloses the use of an insecticidal protein, specifically a method and its application for controlling lepidopteran pests. The method includes contacting the lepidopteran pests with the insecticidal protein, or introducing a nucleic acid molecule encoding the insecticidal protein into a plant, causing the lepidopteran pests to feed on the plant. The insecticidal protein contains the amino acid sequence shown in SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:20. In this invention, the Cry1Da1 proteins BD1-002, BD1-018, BD1-019, BD1-021, BD1-022, and BD1-022S exhibit good insecticidal activity against the soybean pest, the silver-striped armyworm.

The present invention relates to a method of controlling or preventing infestation of a plant by lepidoptera or coleopteran insects, comprising applying a combination or composition to the plant, its locus or its propagation material in any desired sequence or simultaneously, the combination or composition comprises as component (A) a compound of formula (A-1) and as component (B) a compound of formula (B-1). Compositions suitable for use in the invention are also disclosed. (A-1) (B-1)

The invention relates to an in-situ binding method for evaluating binding of Bt toxin Cry1Ac and midgut epithelial cells of cotton bollworm larvae. The method comprises the following steps: incubating and culturing midgut tissues of larvae of lepidoptera pests and a Bt toxin-containing Sf-900 II cell culture solution, carrying out immunostaining, observing and photographing through a laser confocal microscope, and finally carrying out quantitative processing on a fluorescence image by utilizing ImageJ software so as to calculate an average unit area fluorescence signal value. And further evaluating the difference between the binding amounts of the midgut epithelial cells and the Bt toxins of the larvae of different strains. The method can intuitively and accurately observe and evaluate the binding condition of the Bt toxin and the midgut epithelial cells on the premise of retaining the original structure and physiological environment of the midgut tissue of the larvae, provides direct data support for Bt resistance research, assists in analyzing the pest Bt resistance mechanism, has important significance in formulating resistance early warning and treatment strategies, and has a wide application prospect. The method is suitable for the biotechnical fields of Bt toxin insecticidal mechanism research, lepidoptera pest drug resistance mechanism exploration and the like.

This disclosure provides compositions and methods for controlling pests. The methods involve transforming organisms with nucleic acid sequences encoding insecticidal proteins. Specifically, the nucleic acid sequences can be used to prepare plants and microorganisms with insecticidal activity. Therefore, this disclosure provides transformed bacteria, plants, plant cells, plant tissues, and seeds. The compositions are insecticidal nucleic acids and proteins of bacterial strains. The sequences can be used to construct expression vectors for subsequent transformation into organisms of interest, including plants, and as probes for isolating other homologous (or partially homologous) genes. The insecticidal proteins can be used to control, kill, or inhibit the growth of the following pest populations: Lepidoptera, Coleopteran, Dipteran, fungi, Hemipteran, and nematodes, and can also be used to produce compositions with insecticidal activity.

The present disclosure provides nucleic acids obtained from Bacillus thuringiensis strains, and variants and fragments thereof, which encode polypeptides having pesticidal activity against insect pests including Lepidoptera. Particular embodiments of the present disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising the nucleic acids of the embodiments. These compositions can be applied in methods for controlling pests, particularly plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms. These compositions find use in methods for controlling pests, especially plant pests..

The present invention relates to insect neuropeptide compounds of the AKH family and analogs thereof having activity against insects, such as hemiptera, diptera, lepidoptera, cockroaches and / or coleopteran insects, such as aphids and fruit flies, and their use as insect control agents (e.g. Insecticides) and plant protection agents.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

This invention discloses a pesticide composition containing a pyrazole carbamide insecticide and its application. The active component A is a pyrazole carbamide insecticide, and component B is at least one other insecticide defined in the specification. Specifically, the pesticide composition is used to control common plant pests of the Lepidoptera, Coleoptera, Diptera, Thysanoptera, and Homoptera orders.