260 results about "Order Lepidoptera" patented technology

The invention belongs to the technical field of organic matter preparation, and discloses an amide-containing isoxazoline derivative as well as a preparation method and application thereof. According to the invention, chemical modification and molecular design are carried out on the compound with an isoxazoline structure, and a substituent group is introduced to N of an amide group, so that a series of efficient compounds which can be used for killing insects or mites in agriculture or forestry and have excellent activity are obtained, and particularly, the compounds have very good biological activity on lepidoptera, hemiptera and other pests. When being used at an extremely low concentration, the compound disclosed by the invention also shows an excellent control effect on various pests or pest mites, has insecticidal or acaricidal activity in each stage (such as eggs, larvae (nymphs), pupae and adults) of the life cycle of the pests or pest mites, and has an excellent control effect on the pests or pest mites which have drug resistance to traditional insecticides or acaricides. And the composition also shows excellent control activity.

The invention belongs to the field of molecular biology, and particularly relates to a transgenic soybean event LP207-1 and a detection method thereof. The invention provides a nucleic acid sequence for detecting a transgenic soybean event LP207-1, the nucleic acid sequence is selected from one or more of sequences SEQ ID NO: 1-7 or complementary sequences thereof, and the nucleic acid sequence is derived from a plant, a seed or a cell of the transgenic soybean event LP207-1. A representative sample of a seed of the transgenic soybean event LP207-1 is preserved in the China Center for Type Culture Collection on May 23, 2025, and the preservation number is CCTCC NO: P202516. The transgenic soybean event LP207-1 has good resistance to lepidoptera pests and can tolerate agricultural herbicides containing glyphosate and glufosinate-ammonium, economic loss caused by the lepidoptera pests can be avoided, and the yield is not reduced; and the breeding efficiency is enhanced, and the molecular marker can be used for tracking a breeding population and transgenic insertion fragments in offspring of the breeding population. The detection method provided by the invention can quickly, accurately and stably identify the existence of the plant material derived from the transgenic soybean event LP207-1.

The invention discloses a pesticide composition containing a pyrazolecarboxamide pesticide and application of the pesticide composition. The pesticide composition comprises an active component A which is the pyrazolecarboxamide pesticide and a component B which is at least one other pesticide defined in the specification, specifically, the invention relates to a pesticide composition for preventing and treating common lepidoptera, coleoptera, diptera, thysanoptera and homoptera pests of plants.

The invention relates to the technical field of pesticides, and discloses an isoxazoline compound, a preparation method and application thereof, an agricultural composition and a method for preventing and treating invertebrate pests. The invention provides an isoxazoline compound with a structure as shown in a formula (I), or an agriculturally acceptable salt thereof, or a stereoisomer thereof. The isoxazoline compound and the agriculturally acceptable salt thereof provided by the invention show very high prevention and control effects on various pests, especially spider mites represented by tetranychus cinnabarinus, tetranychus urticae, tetranychus shennieri, panonychus citri and the like, and lepidoptera insects represented by plutella xylostella and the like; the acaricidal composition can be used for preventing and treating various pests and harmful mites, has efficient acaricidal and insecticidal activity, and has a good application prospect. # imgabs0 #

The invention relates to the technical field of microorganisms, in particular to serratia marcescens and application of the serratia marcescens in prevention and treatment of lepidoptera pests. The serratia marcescens CB08 provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number of the serratia marcescens CB08 is CCTCC (China Center for Type Culture Collection) NO: M 2025266. The strain has a remarkable prevention and control effect on Lepidoptera pests such as spodoptera frugiperda, armyworm, prodenia litura and cotton bollworm, has no pathogenicity on host crops such as corn, is a bacterium with excellent Lepidoptera pest prevention and control potential, has a very wide application prospect, and is expected to bring a new solution to the field of agricultural pest prevention and control.

The invention discloses a plant anti-lepidoptera pest related gene MIW1 and application thereof, and relates to the technical field of gene engineering, the MIW1 gene is derived from arabidopsis thaliana, the nucleotide sequence of the MIW1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of an encoding protein of the MIW1 gene is as shown in SEQ ID NO.2. The invention proves that the arabidopsis thaliana MIW1 gene can mediate RPX1 to resist plutella xylostella for the first time, and participates in pest resistance by promoting RPX1 protein degradation, so that the plant anti-lepidoptera pest related gene MIW1 can be used for preventing pests. As a new insect-resistant gene of a model plant, the arabidopsis MIW1 gene has high reference value and significance, and provides a new research thought for insect-resistant breeding of economic crops such as oilseed rape, corn, rice, sorghum and the like.

The invention mainly provides Beauveria bassiana ZABb200102 and application of the Beauveria bassiana ZABb200102, the Beauveria bassiana ZABb200102 is preserved in China General Microbiological Culture Collection Center on October 28, 2024, and the preservation number of the Beauveria bassiana ZABb200102 is CGMCC (China General Microbiological Culture Collection Center) No. 41557. The strain is separated from sick lepidoptera cacalidae larva bodies for the first time, and it is found through toxicity determination that the strain has high pathogenicity to tomato leaf miner larvae. In addition, the strain can also be colonized in tomatoes, so that the growth of the tomatoes is promoted, and the resistance of the tomatoes to pests is indirectly improved. Therefore, the strain can be widely applied to development of tomato bacterial fertilizer and green prevention and control of tomato leaf miner which is an important tomato pest.

The invention discloses an RNA (Ribonucleic Acid) interference method for parasitic wasps in lepidoptera larvae, which is characterized in that RNA interference is carried out by using a microinjection method or a nano-material immersion method when the parasitic wasps in the lepidoptera larvae are not separated from hosts or are separated from the hosts to cocoon, so that an RNAi system of the parasitic wasps is successfully constructed, and the defects that the parasitic wasps are small in size in an egg stage, and the parasitic wasps are not damaged in the egg stage can be effectively overcome. And RNAi operation difficulty caused by cocoon wrapping in the pupal stage and short life in the adult stage is avoided. Moreover, the method disclosed by the invention is simple to operate and high in interference efficiency, provides a theoretical basis and a reference effect for further researching gene functions of the parasitic wasps, and has important significance on biological control.

The invention relates to the technical field of spiro compounds, in particular to an 8, 8-difluoro-2-oxaspiro [4, 5] decane-1, 3-diketone compound as well as a preparation method and application thereof. The invention provides the 8, 8-difluoro-2-oxaspiro [4, 5] decane-1, 3-diketone compound, the compound shows excellent biological activity, and through test comparison, when the mass concentration is 0.1 mu g / mL, the insecticidal effect of the compound is increased by nearly 10% compared with that of a common insecticide chlorantraniliprole. It means that the compound shows a more efficient insecticidal effect on lepidoptera pests such as plutella xylostella and the like under a lower dosage, and cost reduction and synergism are realized. Due to the characteristic, the compound or the agronomically acceptable salt thereof shows important potential application value in the field of insecticides, and a new thought is provided for upgrading and updating of insecticides in the future.

The invention discloses an insect-resistant transgenic cotton event BrsC35 as well as a detection method and application thereof. According to the invention, an epsps gene, a Cry1Ac gene and a Vip3A gene are transferred into a genome of Zhongmiansui 24 through an agrobacterium-mediated method, so that a transgenic cotton event BrsC35 of which the resistance to lepidoptera insects such as cotton bollworms, ostrinia nubilalis and spodoptera frugiperda is remarkably improved is obtained. The transgenic cotton event BrsC35 is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 17, 2025, and the preservation number is CGMCC No.32181. The transgenic cotton event BrsC35 has been preserved in China General Microbiological Culture Collection Center (CGMCC). The invention also provides a method for identifying whether a plant sample is derived from the transgenic cotton event BrsC35 or an offspring of the transgenic cotton event BrsC35, the transgenic cotton event BrsC35 can be specifically detected, and the transgenic cotton event BrsC35 can be better supervised and managed. The method has an important application value.

The present invention relates to insect neuropeptide compounds of the family of pyroglutamic peptides and analogs thereof having activity against insects, such as Hemiptera, Diptera, Lepidoptera, Blattaria and / or Coleoptera insects, such as aphids and fruit flies, and their use as insect control agents (e.g. insecticides) and plant protection agents.

The invention discloses a scotogramma trifolii specific SS-COI primer pair and a rapid molecular detection method, an amplified fragment of the primer pair is a specific region of 227bp in a scotogramma trifolii COI gene, rapid detection and identification of scotogramma trifolii in lepidoptera insects can be realized by using the primer pair, sequencing and sequence alignment are not needed, and the primer pair can be used for rapidly detecting and identifying the scotogramma trifolii in the lepidoptera insects. The kit has the advantages of high sensitivity, strong specificity, simplicity and convenience in operation, good repeatability and the like.

A Vip3Aa mutant protein and the use thereof in controlling pests. The mutant insecticidal protein Vip3Aa contains an amino acid sequence having the following mutations compared with the amino acid sequence as shown in SEQ ID NO: 1: in the amino acid sequence corresponding to that as shown in SEQ ID NO: 1, the amino acid at position 24 is mutated from tyrosine into any other amino acid; the amino acid at position 27 is mutated from alanine into any other amino acid; the amino acid at position 36 is mutated from methionine into any other amino acid; and / or the amino acid at position 28 is mutated from phenylalanine into any other amino acid. The Vip3Aa mutant protein has a significantly improved insecticidal activity compared with a wild-type Vip3Aa, and has a good insecticidal effect on Lepidoptera insects such as Spodoptera frugiperda.

The invention provides a lepidoptera pest egg mass lossless collection method and a portable device based on vibration separation, and relates to the technical field of pest egg mass collection, the portable device comprises a bottom plate, a supporting rod, a side plate and a fixing piece, a sliding mechanism is arranged at the rear end of the bottom plate, a rotating mechanism is arranged in the side plate, and the sliding mechanism comprises an entering cavity, an arc-shaped hopper, an arc-shaped partition plate, a storage cavity, a screen and the like. The rotating mechanism comprises a metal sliding block, a rotating shaft, a connecting piece, a support, an annular magnet and the like, and is further provided with a rotating disc, a spring, a brush and the like. By means of the structure, after soil blocks with eggs are placed into the entering cavity and separated through the arc-shaped partition plate, the rotating disc is rotated to enable the rotating shaft to drive the support to rotate, the metal sliding block moves up and down to generate vibration, the soil blocks are stripped through the adjustable brush, and the egg blocks fall into the containing cavity through the screen; the lepidoptera pest egg masses in soil blocks with different viscosities can be conveniently, efficiently and nondestructively separated and collected, and the device is suitable for the field environment.

The invention relates to the field of insect prevention and control, in particular to a spodoptera frugiperda ABCC4 gene, application thereof and a method for preventing and controlling spodoptera frugiperda. The effect of the ABCC4 gene in the growth and development process of the spodoptera frugiperda is found for the first time, the death rate of larvae of the spodoptera frugiperda can be remarkably increased by inhibiting expression of the ABCC4, population development declines finally, a new thought is provided for controlling development of the population of the spodoptera frugiperda, and the application has a wide application prospect. And a basis is provided for realizing green prevention and control of spodoptera frugiperda and other lepidoptera insects. By applying an RNA interference technology, it is found that the death rate of larvae of spodoptera frugiperda is remarkably increased by inhibiting expression of the ABCC4 gene of the spodoptera In addition, the death rate of the larvae of the spodoptera frugiperda is obviously increased by spraying dsRNA of an interference sequence of the ABCC4 gene of the spodoptera frugiperda encapsulated by a nano material.

The present invention relates to analogues of insect neuropeptides that are active against insects, such as Diptera, Hemiptera, Blattodea and / or Lepidoptera, and to the use of these as insect control agents (e.g., insecticides) and plant protection agents.

The invention discloses application of a plutella xylostella calmodulin inhibitor in prevention and treatment of lepidoptera pests. According to the invention, an inhibitor Cyclosporin A capable of inhibiting the activity of Plutella xylostella calcineurin is used for feeding Plutella xylostella larvae, and it is found that Cyclosporin A can cause the death of Plutella xylostella larvae and reduce the survival rate of the larvae. In addition, the Cyclosporin A treatment can prolong the pupation time of the plutella xylostella and the eclosion time of pupae, greatly reduce the pupation rate and the eclosion rate, and also can obviously reduce the egg laying amount of female plutella xylostella and the hatching rate of eggs. On the basis, the plutella xylostella can be prevented and controlled by utilizing the substance capable of inhibiting the activity of the calmodulin of the plutella xylostella. Meanwhile, the calmodulin is relatively conservative in the lepidoptera insects, so that substances capable of inhibiting the activity of the calmodulin of the plutella xylostella can also be used for preventing and treating other lepidoptera insects. The invention provides a new target for prevention and treatment of lepidoptera pests, and is beneficial to green prevention and treatment of lepidoptera pests.

The present invention discloses a pest trapping device for selectively protecting natural enemies. By utilizing the living habits of Lepidoptera pests, it attracts adult cotton bollworms, adult beet armyworms, adult Spodoptera litura, adult cotton leafrollers, adult cutworms and other Lepidoptera pests for capture to reduce the number of pests. At the same time, by arranging insect-blocking teeth pointing to the center of the attracting cavity on the inner edge of the insect inlet groove of the trapping device, it does not affect the entry of pests into the attracting cavity while preventing pests from escaping from the insect inlet groove. Natural enemy insects such as Adonia variegata, Coccinella undecimpunctata, Stethorus punctillum Weise, and Nabis sinoferus Hsiao, which are smaller in size than pests, can freely enter and exit. While trapping pests, it protects natural enemy insects, solves the problem that existing pest trapping devices are prone to capture natural enemy insects together when trapping pests, resulting in a simultaneous reduction in the number of pests and natural enemy insects and an insignificant control of the pest population, and achieves the purpose of protective biological control, having broad popularization and application value in the field of pest trapping technology.

The present invention relates to an insecticidal composition comprising of N-(phenylthio) phthalimide (NPP), neem oil and chitosan and method of preparation thereof. The composition of the present invention is useful against insects selected from Helicoverpa armigera, Mythimna separata, Spodoptera frugiperda and other Lepidopteran species of insects. The composition can serve as a formulation that possesses a lower probability of acquiring resistance.

The invention belongs to the technical field of organic matter preparation, and provides an isoxazoline compound containing benzamide as well as a preparation method and application thereof.Chemical modification and molecular design are performed on a compound with an isoxazoline structure, a substituent group is introduced to N of an amide group, and the benzamide-containing isoxazoline compound is prepared. A series of high-efficiency compounds which can be used for killing insects or mites in agriculture or forestry and have excellent activity are obtained, and particularly, the compounds have very good biological activity on pests of lepidoptera, hemiptera and the like. When being used at an extremely low concentration, the compound disclosed by the invention also shows an excellent control effect on various pests or pest mites, has insecticidal or acaricidal activity in each stage (such as eggs, larvae (nymphs), pupae and adults) of the life cycle of the pests or pest mites, and has an excellent control effect on the pests or pest mites which have drug resistance to traditional insecticides or acaricides. And the composition also shows excellent control activity.

The application discloses application of a microbial natural product GameXPeptide-A in improving the control effect of insecticides on pests, and belongs to the technical field of biological control. Experiments prove that GameXPeptide-A can effectively improve the insecticidal activity of insecticides on agricultural pests such as thrips, corn borer and prodenia litura when GameXPeptide-A is combined with insecticides such as Metarrhizium anisopliae, Beauveria bassiana, nuclear polyhedrosis virus, 4% abamectin and pyridaben, 10% dimethoate and spinosad, 30% ethoxazole, 45% bifenthrin and etoxazole, 5% spinosad, 5% chlorantraniliprole, 5% abamectin and the like, and it is indicated that the substance can be combined with different insecticides to improve the insecticidal activity of insecticides on pests such as Lepidoptera, Diptera, Coleoptera, Orthoptera, Hemiptera and Homoptera in agricultural production, and can be used for the development and application of a new type of pest immunosuppressant or insecticide synergist.

The present disclosure provides compositions nanocomposite biopesticides exhibiting improved efficacy, photostability, as well as controlled release and systemic delivery characteristics and methods of making and using the same. The present disclosure further provides methods for protecting a plant from a Lepidopteran pest as well as controlling a Lepidopteran pest.

The invention discloses a parasitic wasp-friendly multifunctional trichoderma asperellum ZJ12 and application thereof, the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: M2025805, and the strain has broad-spectrum and efficient antagonistic activity; various phytopathogens such as fusarium oxysporum, fusarium solani, fusarium pseudograminearum, verticillium dahliae, tea pestalotiopsis, phytophthora nicotianae, northern leaf spot helminthosporium bombycis and globulosporium melanocarpum are efficiently inhibited, and a good prevention and treatment effect is shown in a potting prevention effect experiment; the biocontrol microbial inoculum shows transboundary prevention and control potential to lepidoptera pests (plutella xylostella and cabbage caterpillars) and has good biological safety to natural enemy insect parasitic wasps (chrysalis pupae and lateral cotesia manilensis), and the problem that an existing biocontrol microbial inoculum is single in function is effectively solved.

This invention discloses a natural plant-extracted biological insecticide and its preparation and application methods, belonging to the field of agricultural biotechnology. The insecticide uses cypress leaves, stemona root, clematis root, pinellia root, and ailanthus leaves as raw materials, prepared through boiling, cooling, filtration, sealed maturation, double-barrel water bath temperature gradient concentration, and pasteurization. This insecticide kills pests by sealing their spiracles and rapidly penetrating their bodies, causing suffocation. It primarily works through contact, exhibiting good residual effect, low resistance development, no chemical residues, and is environmentally friendly. The preparation process is mild, maximizing the retention of heat-sensitive active ingredients. The product is highly stable and low-cost, particularly suitable for controlling Diptera, Coleoptera, Orthoptera, Hemiptera, and / or Lepidoptera pests.

The invention belongs to the field of biological control, and relates to application and a method of dsRNA of an NRX gene in control of lepidoptera pests. It is found for the first time that the Neurexin-4 gene participates in the growth and development process of the cotton bollworm and the spodoptera frugiperda, the death rate of larvae of the cotton bollworm and the spodoptera frugiperda is remarkably increased through deletion of Neurexin-4 expression, and finally population development is inhibited. The gene can be used for developing RNA biopesticides for preventing and controlling cotton bollworms and spodoptera frugiperda. The invention provides an interference sequence for the cotton bollworm and grassland spodoptera Neurexin-4 gene, the sequence can inhibit the expression of Neurexin-4, and the sequence can be utilized to interfere the expression of the cotton bollworm and grassland spodoptera Neurexin-4 gene and inhibit the growth of the cotton bollworm and grassland spodoptera Neurexin-4 gene.

The present invention relates to a method o f combating and / or controlling a pest selected from the Lepidoptera order, which comprises applying to said pest, to a locus of said pest, to a plant susceptible to attack by said pest, or to a plant propagation material susceptible to attack by said pest, a pesticidal composition comprising isocycloseram as active ingredient A, and cartap as active ingredient B.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The invention discloses a lepidoptera pest catching device, and belongs to the technical field of pest control. The device is mainly composed of a base, a rectangular base frame, a rectangular frame, a trap lamp, a top box, a strip-shaped overturning piece, an overturning mechanism, an insecticide storage box and related auxiliary assemblies. The area of the rectangular frame is large so that the insect catching amount can be increased. After pests enter, the overturning mechanism drives the narrow strip-shaped overturning piece to rapidly and synchronously rotate, the frame is closed, meanwhile, the sliding plate synchronously seals the discharging hopper, the response time of the pests is greatly shortened, and the pests are effectively prevented from escaping. After closing, the insecticide is treated by the atomizer and uniformly fills the frame under the action of the fan and the guide plate, and the high-concentration insecticide can thoroughly kill insects and reduce the dosage. Insect corpses fall into the discharging hopper through the through holes in the bottom of the rectangular frame and are finally discharged through the guide-out plate. The device has the advantages of being high in insect catching efficiency, thorough in insect killing, capable of saving energy, environmentally friendly and the like, and is suitable for lepidoptera pest control work in various scenes such as farmlands, orchards and flower beds.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

The present invention relates to natural or natural-like analogues of insect adipokinetic hormone (AKH) neuropeptide [Pyr]-LTFTSSWGG-[NH2] (SEQ ID NO:2) having activity against insects, for example hemipteran, dipteran, lepidopteran and / or coleopteran insects, such as aphids, moths and fruit flies, and their use as insect control agents (e.g. insecticides) and plant protection agents.