21 results about "Emamectin" patented technology

The invention discloses a megalurothrips megalurothrips methylamino abamectin benzoate resistance molecule detection method which comprises the following steps: (1) collecting megalurothrips megalurothrips female adults in a plurality of regions, and extracting DNA (deoxyribonucleic acid) in a single region in a mixed manner; (2) designing glutamic acid gated chloride ion channel gene specific primers of the megalurothrips megalurothrips, respectively adding specific tags at the front ends of the primers in all regions, and carrying out PCR (Polymerase Chain Reaction) amplification; mixing the PCR products of each region to obtain a library; (3) performing second-generation amplicon sequencing on the library to obtain off-machine data; and (4) analyzing offline data, merging the data in the same region, calculating the mutation frequency of each region by taking the ratio of the mutation (G232S) of the target sequence as an index, and judging the resistance of the megalurothrips usitatus to the emamectin benzoate. The method can be used for rapidly and massively monitoring the emamectin benzoate resistance condition of the field megalurothrips megalurothrips, and an important basis is provided for high-level emamectin benzoate resistance early warning and megalurothrips megalurothrips chemical treatment.

This invention relates to the fields of pesticide formulations and pesticide pest control, specifically to a composite nano-slow-release suspension of emamectin benzoate and chlorantraniliprole, its preparation, and application. The nano-slow-release suspension comprises emamectin benzoate, chlorantraniliprole, a nanocarrier, a wetting agent, a dispersant, a synergist, a thickener, an antifoaming agent, an antifreeze, a preservative, and water. The nanocarrier is an NX-CMCS & MOF composite nanocarrier. Spraying this composite nano-pesticide onto crops improves the adhesion and penetration of the pesticide on leaves, enhances its resistance to rain washout, and effectively kills major crop pests such as bollworms, diamondback moths, cowpea thrips, and rice planthoppers. Compared with traditional pesticide formulations, it has a significant effect of reducing dosage and increasing efficacy, and has broad application prospects in the field of agricultural pest control and promoting green agriculture.

The present invention relates to combinations of a compound of formula (A-1) with a compound selected from the group consisting of dimpropyridaz, emamectin and isocycloseram, bentioflumin, pioxaniliprole, isoflualanam, cybenzoxasulfyl, vinylfluthrin, cyclobunofen, flupymezotiaz, fentiazoluron, 1-(1,2-dimethylpropyl)-5-methyl-N-prop-2-ynyl-N-(3-pyridyl)pyrazole-4-carboxamide, 1 -[4- [5-ethylsulfonyl-1-methyl-4-[3-methyl-6-(trifluoromethyl)imidazo[4,5-b]pyridin-2-yl]imidazol-2- yl]phenyl]cyclopropanecarbonitrile, [(Z)-[[3-ethylsulfonyl-5-[4-(trifluoromethoxy)phenyl]-2-pyridyl]-[[5- (trifluoromethyl)-2-pyridyl]amino]methylene]amino] acetate, 3-[3-ethylsulfonyl-5-[4- (trifluoromethoxy)phenyl]-2-pyridyl]-4-[5-(trifluoromethyl)-2-pyridyl]-1,2,4-oxadiazol-5-one, 1-(4- chlorophenyl)-2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfanyl)benzene, and emamectin benzoate; and to methods of controlling or preventing damage to plants using such combinations; and to formulations, concentrates and seed treatments comprising such combinations.

The invention discloses an emamectin benzoate nano-pesticide with both leaf adhesion and pH response as well as a preparation method and application of the emamectin benzoate nano-pesticide, and the nano-pesticide is prepared from a composition for the nano-pesticide. Comprising the following components in parts by weight: 2-15 parts of emamectin benzoate, 30-80 parts of a copolymerization surfactant, 4-15 parts of a nonionic surfactant, 10-60 parts of sodium alginate and 2-15 parts of a cross-linking agent. The nano-pesticide disclosed by the invention is slowly released in a neutral environment (pH is 7) and quickly released in a pest midgut alkaline environment (pH is 9-10), the release rate within 12 hours reaches 60% or above, the nano-pesticide can accurately act on target pests, the pesticide effect is improved, and the non-target toxicity is reduced.

The invention relates to the technical field of pesticide dry suspending agents, in particular to an emamectin benzoate nano dry suspending agent as well as a micro-fluidic pilot plant test preparation method and application thereof. The invention provides a preparation method of an emamectin benzoate (EMB) nano dry suspending agent, and the method comprises the following steps: (1) providing an organic phase which contains emamectin benzoate, a nano carrier, an organic solvent and an optional non-aqueous auxiliary agent; (2) providing a water phase, wherein the water phase contains water and a water-based auxiliary agent; and (3) mixing the organic phase and the water phase in a micro-mixing channel, removing the organic solvent, and drying to prepare the emamectin benzoate nano preparation.

This invention provides a method for preparing and applying a controlled-release agent of emamectin benzoate, belonging to the field of nanopesticide technology. This invention uses PFC-1, a hydrogen-bonded organic framework material with a porous structure, as a drug carrier. After loading emamectin benzoate through its porous structure, the loaded system is further modified by surface encapsulation with sodium lignosulfonate. The resulting nano-formulation with controlled-release function is called emamectin benzoate nano-controlled-release agent. The prepared drug-loaded system exhibits good insecticidal activity against diamondback moth. The preparation method includes a method for preparing emamectin benzoate-loaded agents. The prepared system has a high loading capacity of 19.6%. The drug-loaded system provided by this invention has good insecticidal effect against diamondback moth and exhibits pH-selective release effect in the midgut environment of the diamondback moth.

The present invention relates to a composition comprising: (a) isoxazolam, (b) emamectin methylaminoazine, and (c) a sulfonated lignin compound with a molecular weight up to 10,000 g / mol.

The invention provides a pesticide composition containing antibiotic compounds, the pesticide composition is composed of a first effective component and a second effective component, the first effective component is a compound with a structural formula (I), the second effective component is methylamino abamectin benzoate, abamectin, spinosad, spinetoram or ivermectin, and the weight ratio of the first effective component to the second effective component is (1-50): (1-50). The invention also provides application of the pesticide composition in prevention of agricultural and forestry pests. The invention verifies that the binary composition obtained by compounding the flufenutraniliprole and the antibiotic insecticide in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips and flea beetle. (I).

The invention provides an insecticidal composition containing an active component A and an active component B. The active component A is emamectin benzoate, the active component B is a compound shown as a formula I (formula I), the mass ratio of the active component A to the active component B is (1-30): (1-30), the insecticidal composition further comprises an auxiliary agent, the active component accounts for 1%-30% by weight of the insecticidal composition, and the auxiliary agent accounts for 1%-30% by weight of the insecticidal composition. The insecticidal composition is used for preventing and treating agricultural pests, has an obvious synergistic effect, and can reduce the dosage and delay the occurrence of pest resistance to drugs.

The present disclosures present an insecticidal composition comprising a combination of active ingredients, Emamectin benzoate, Cypermethrin and Cartap hydrochloride, and methods of preparing and using such compositions. The present disclosure aims to provide enhanced control of a variety of insect pests affecting crops, with a synergistic effect that improves efficacy compared to individual components.

The present invention relates to a method of combating and / or controlling a pest selected from the family Noctuidae, comprising applying a pesticidal composition to said pest, a locus of said pest, a plant susceptible to said pest or plant propagation material susceptible to said pest, the pesticidal composition comprises isoxaflutole as an active ingredient A and emamectin as an active ingredient B.

The present application relates to the technical field of step-by-step synthesis reaction, in particular to a step-by-step synthesis reaction method of emamectin benzoate, which extracts the heat trace path of raw materials in the preheating section and forms a propulsion trajectory line, selects a related heat band in the middle section to generate a conversion path diagram, positions each path and connects the conversion fluctuation section to form a rhythm structure, reflects the main and auxiliary material route adjustment entrance to realize collaborative propulsion, and connects the whole section to form a synthesis path paragraph set along the propulsion direction. The present application can form a comparable spatial clue by converting the heat diffusion change of the raw material after touching the wall into a path sequence, continuously track the reaction progress by observing the energy fluctuation and position change of the path in the middle section, present the stage connection rhythm by associating the start and end points of each path section, and facilitate the identification of the transition urgency. By comparing the main and auxiliary material paths, the approach area deviation can be captured to improve the consistency of the entry section. By integrating all path sections along the propulsion direction, the continuous coupling of multi-section reactions can be enhanced and the deviation can be reduced.

The present invention belongs to the technical field of organic synthesis and fine chemical engineering, and particularly relates to an alcohol ether compound and the use thereof in a water-based nano pesticide preparation. The alcohol ether compound provided in the present invention has a structure as shown in formula 1. A long branch chain is present in the molecular structure of the alcohol ether compound provided in the present invention, such that self-assembly is easy to achieved, and the branching of a non-polar group makes the hydrophilicity and lipophilicity of the compound be close to a balance, such that a microemulsion system can be spontaneously formed in water; in addition, the molecular weight thereof is small, and a hydrophilic group is located in the middle of the molecular chain, such that the wetting effect is good; and the hydrophilic group has more oxygen atoms, such that the hydrophilicity thereof is higher. When the compound provided by the present invention is used as a specific surfactant of emamectin benzoate, the stable and transparent emamectin benzoate nano water-based preparation can be obtained in water with the emamectin benzoate at room temperature without using any organic solvent, and the compound has the advantages of low environmental pollution, low toxicity to human and livestock and resource saving.

The invention discloses a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection method based on a cytochrome P450 gene CYP9G2 and a matched kit, which are used for rapidly and accurately detecting the drug resistance of plutella xylostella to emamectin benzoate. According to the method, whether a plutella xylostella individual or population has resistance to emamectin benzoate or not is judged by quantitatively analyzing the mRNA expression level of a CYP9G2 gene. The kit comprises a specific primer pair, and the sequences of the specific primer pair are respectively SEQ ID NO.1 (forward) and SEQ ID NO.2 (reverse). The method is easy and convenient to operate and rapid in detection, sample treatment to result analysis can be completed within 3-4 hours, and the method is remarkably superior to a traditional biological determination method. The application of the method can realize early discovery and dynamic monitoring of the drug resistance of field plutella xylostella, provides a direct basis for scientific use and resistance treatment of emamectin benzoate in vegetable production, is beneficial to reduction of the pesticide use amount, reduction of the control cost and guarantee of the vegetable quality safety, and has important practical popularization value in the aspects of agricultural green production and pesticide application reduction.

The application provides a suspension agent containing emamectin benzoate and indoxacarb. The suspension agent uses a compound thickening agent, cooperates with a specific antifreezing agent, determines a proper raw material adding sequence and preparation steps, and process parameters such as shearing time and grinding temperature, so as to avoid the situations such as clumping or caking and decomposition of active ingredients which are prone to occur in the preparation process of the suspension agent, and is suitable for large-scale industrial production. The suspension agent has good stability and remarkable drug efficacy. Field tests show that the suspension agent has excellent control effect on lepidopteran pests, has long effective period, is safe to crops, and is environment-friendly.

The invention provides an insecticidal composition for preventing and controlling thrip pests and a comprehensive prevention and control method for sunflower grain rusty spots, and relates to the technical field of plant disease and pest prevention and control. The insecticidal composition for preventing and controlling the thrip pests comprises at least two of the following components: a spinosad emulsion in water, an abamectin.spirotetramat suspending agent, a afidopyropen dispersible liquid, an emamectin benzoate-pyriproxyfen suspending agent and a spinetoram suspending agent. The insecticidal composition is independently or jointly used for physically preventing and controlling a monochromatic plate with an attraction effect on thrip pests and biologically preventing and controlling orius sativus, and the formed comprehensive prevention and control method not only can effectively inhibit the rust disease index of sunflower seeds, but also does not affect the yield of sunflowers.

The invention provides a construction method and application of a P450 fusion protein. The construction method comprises the following steps: step (1), fusing abamectin oxidation type P450 Ema1 with a Rhodococcus P450RhF-RPs reductase structural domain by adopting a connecting peptide to obtain Ema1-Rhf; and (2) carrying out point mutation on the Ema1-Rhf, and constructing the P450 fusion protein. According to the invention, redox partners derived from Rhodococcus are connected with a structural domain of P450 Ema1 through a designed connecting peptide; the cytochrome P450 monooxygenase mutant further improves the enzyme activity in the process of catalyzing abamectin to be converted into methylamino abamectin, the maximum conversion rate can be improved by 2.2 times through determination, and the mutant achieves 90% conversion rate within 39.8 hours, which is far better than the expression of an unfused system. And the method has certain significance on industrial production of the emamectin benzoate.

The invention relates to the technical field of pesticide compounding, in particular to a compound pesticide of emamectin benzoate and lufenuron, which comprises the active ingredients of emamectin benzoate and lufenuron. The synergistic effect is evaluated through the co-toxicity coefficient (CTC), when the CTC is larger than or equal to 120, it is judged that the synergistic effect is remarkable, the mass ratio of the preferable effective components is 2: 1, 1: 1, 1: 2, 1: 3 or 1: 4, the optimal effect is shown according to the ratio of 1: 2, the co-toxicity coefficient reaches 160.56, liquid medicine with the concentration gradient of 2.4 mg / L, 1.2 mg / L, 0.6 mg / L, 0.3 mg / L and 0.15 mg / L is adopted in a test, and spraying is conducted through a Potter spray tower at the liquid spraying amount of 1 mL and the pressure of 1.47 * 10 < 5 > Pa. The LC50 value of the compound insecticide for citrus phyllocoptruta oleivora is 0.23-0.45 mg / L, and the LC90 value is 2.42-3.40 mg / L, which indicates that the efficient control effect can be realized at a lower concentration, the pesticide dosage is reduced, and the environmental pollution risk is reduced.

The application discloses a method for nanometerizing emamectin benzoate. The method comprises the following steps: mixing an organic acid or an organic acid salt buffer solution with a solution of emamectin benzoate to obtain nanometerized emamectin benzoate; wherein the molar ratio of emamectin benzoate to the organic acid is 1:(10-30); and the molar ratio of emamectin benzoate to the acid radical ion in the organic acid salt buffer solution is 1:(10-30). By adding 10-30 times of the molar ratio of the organic acid or the organic acid salt buffer solution to the solution of emamectin benzoate, the emamectin benzoate can be nanometerized to form a micellar solution, the solubility and effective content of the emamectin benzoate in water are greatly improved, and no organic solvent needs to be added in the later period. The nanometerized emamectin benzoate prepared by the method has small particle size, which can reach 6-8 nm, and uniform particle size.

The invention discloses a compound composition of emamectin benzoate and dsRNA (double-stranded ribonucleic acid) and application of the compound composition in prevention and control of nematodes. According to the invention, dsRNA is respectively designed and synthesized for five Par genes of bursaphelenchus xylophilus, and nucleotide sequences of positive-sense strands of the dsRNA are respectively shown as SEQ ID No.1-SEQ ID No.5; the synthesized dsRNA and emamectin benzoate of different components are compounded to obtain the pesticide compound composition, the pesticide compound composition shows a remarkable synergistic effect in the aspect of preventing and treating pine wood nematode diseases, and the nematode killing effect of combined use of the dsRNA and the emamectin benzoate is superior to that of single use of any agent; the pesticide compound composition has the potential to become a novel trunk injection for preventing the pine wood nematode disease.

The invention discloses a composition containing emamectin benzoate and spinetoram, a preparation and application of the composition and the preparation. The microemulsion comprises an active component, a conventional auxiliary agent and a key functional systemic accelerator. An organic silicon surfactant (such as Silwet L-77) and methylated vegetable oil (such as Pro-MSO) are compounded according to a specific proportion to serve as a functional uptake accelerant, the combination can generate a synergistic effect, and uptake conductivity of active ingredients in plants is remarkably improved. Indoor biological assay results show that the LC50 value of the preparation is reduced by more than 50% compared with that of a traditional formula; field pharmacodynamic tests prove that under the condition of the same dosage of the effective components, the control effect on the eggplant thrips reaches up to 95% or above, and the persistence is excellent. According to the invention, through innovative optimization of an auxiliary system, synchronous improvement of the pesticide utilization rate and the control effect is realized, and an effective scheme is provided for developing a novel pesticide preparation which is efficient, low in dosage and capable of delaying resistance.