56 results about "Beauveria" patented technology

Microsclerotia of entomopathogenic fungi, including Metarhizium species, Beauveria species, and Lecanicillium species, may be produced. These microsclerotia are effective for the control of a wide variety of insect pests, particularly soil-dwelling insect pests.

A green efficient seed dressing agent is provided by the present invention, and belongs to the technical field of pesticide. The green efficient seed dressing agent consists of pure natural plant extracts such as brassinosteroid, algaeinc and tea saponin, and bio-functional bacteria such as Bacillus subtilis, actinomycetes, Beauveria bassina, trichoderma and Bacillus thuringiensis. The seed dressing agent is green and environmentally friendly, and has good effects on improving the germination rate and emergence rate of seeds, and on insect killing and sterilization.

The invention relates to a preparation method and application of q beauveria-spinosad suspending agent. Beauveryia bassiana and spinosad are the active ingredients of the suspending agent which is used for preventing and treating agriculture and forestry injurious insects, such as leptinotarsa decemlineata and acridid and the like. The invention is characterized in that (1) the suspending agent comprises active ingredients, namely the beauveryia bassiana and the spinosad; and (2) the mass ratio of the beauveryia bassiana and the spinosad is (30:1)-(30:2), and the mass percent of the effective components in the preparation is 3.1-10%. The preparation method has the advantages that the product can be dissolved with oil or water; the effective duration of the beauveria vuillemin and the relative quick action of spinosad have complementary advantages, and injurious insects of various crops are treated; and the suspending agent prepared by the method has the advantages of good synergism and permeability, strong adhesivity and evaporation resistance and the like.

Disclosed is a biological composite fertilizer, which is multiple-effect in water- holding and insect-protected for trees and vegetation. It is introduced in accordance with two problems: mono-nutrient, the slow promotion of the plants growth and low resistance to the outer injury of the natural organic domestic animal manure fertilizer; severe damage to the soil structure if used for a long time despite its strong promotion to plants growth, the bad taste of the fruit, reduction of nutrition and some pollution of the inorganic compound fertilizer; which can meet the demand of ecological construction in accordance with the western exploitation strategy in our country. It is characterized in that taking natural peat as the main component for the water-holding effect; taking domestic animal manure as necessary organic fertilizer; adding azotobacter, phosphobacteria, potassiumbacteria, Mongolianmushroombacteria, beauveria and green muscardine fungus as immuno-biological insect protected;mixing with some certain quantity inorganic salt and a little rare earth and preparing to get the product. The main advantage is the full and sufficient effect of the fertilizer, the good effect of water-holding, and strong effect of the anti-pest, and it is mainly used in ecological construction and soil improvement.

The invention discloses a method for preventing and controlling damage of stem borers and relates to the technical field of biological prevention and control of the damage of the stem borers. The method for preventing and controlling damage of the stem borers comprises the following step: releasing a mixture of trichogramma eggs and beauveria spore powder to a target prevention and control area soas to prevent and control target stem borers, wherein the target stem borers are european corn borers or rice stem borers; the mixture released to the target prevention and control area every time per hectare contains 12000-15000 trichogramma eggs and 0.18-0.2g of beauveria spore powder. The method disclosed by the invention has the beneficial effects that by control for the proportion of the trichogramma eggs and the beauveria spore powder, the damage of the stem borers can be effectively controlled, and the use times and the use amount of pesticide and beauveria can be reduced, so that notonly is the labor intensity of farmers reduced, but also the prevention and control cost is reduced, the effects of increasing the yield and the income of the farmers and improving the quality of agricultural products are realized, and the ecological, social and economic benefits are good.

The invention discloses a matrine and beauveria brongniartii combined insecticide and application thereof. According to the matrine and beauveria brongniartii combined insecticide, a beauveria brongniartii strain SB010 is preserved in the Guangdong culture collection center on nineteenth April, 2018, and the preservation number of the beauveria brongniartii strain SB010 is GDMCC NO:60359. The insecticide is prepared from main active components of matrine and the beauveria brongniartii. According to the matrine and beauveria brongniartii combined insecticide and application thereof, research finds that a combination of the matrine and the beauveria brongniartii has a significant synergistic interaction effect, the usages of the matrine and the beauveria brongniartii can be significantly reduced, the use cost is reduced, the insecticide is environmentally friendly, no drug resistance is generated, the insecticide has very high application potential for biological control of prodenia litura, the environmental negative effect problem brought by long-term use of chemical pesticides is solved, moreover, the insecticide has the advantages of being green, environmentally friendly, pollution-free and long in lasting time, and thus the application prospects are very good.

The invention discloses a Beauveria bassiana strain and application thereof. The Beauveria bassiana strain belongs to the class of Beauveria bassiana, the preservation unit is the China General Microbiological Culture Collection Center (CGMCC), the preservation date is December 28, 2018, the preservation number is CGMCC No.17068, and Beauveria bassiana belongs to fungus, deuteromycota, hyphomycetes, moniliales, moniliaceae, beauveria. According to the application, the Beauveria bassiana strain is applied to the preparation of a biological preparation for preventing and controlling euwallacea fornicatus.

The invention relates to a dong-shaped Chinese potato endogenetic fusarin producing beauveria. The endogenetic fungal Dzf2 incorporating the invention is obtained from dong-shaped Chinese potato by endogenetic fungal isolation technology, and is classified and determined as fusarium by tiny organism. The bacterial strain has been preserved in china tiny organism culture preservation management committee commonness tiny organism local and accession identification serial number is CGMCC No.2340. The endogenetic fungal producing beauveria is first separated from dong-shaped Chinese potato and the antibacterial activity of beauveria is definituded. The object of the invention is to obtain savageness activity ingredient via plants endogenetic fungal resource.

The invention discloses a recombinant beauveria brongniartii proteinase K mutant PK-M1 and a preparation method of the recombinant beauveria brongniartii proteinase K mutant PK-M1. According to the technical scheme, starting from transforming the molecular structure of proteinase K of recombinant beauveria brongniartii, the proteinase K mutant PK-M1 with the activity and stability both improved isobtained, and the amino acid sequence is as shown in SEQ ID NO:1; an expression system is optimized, by utilizing the yeast cell external secretion expression technology, the recombinant proteinase Kmutant can be fermented in large scale and efficiently expressed, and further, the yield of proteinase K is improved; and with the protein purification manner realizing efficient affinity chromatography, the yield of the proteinase K in the purifying process is improved, and thus the large-scale production of the proteinase K is realized.

A process for preparing non-woven disinfecting strips of Beauveria globisporus includes sampling its bacterial strain, slant culture, liquid culture, production with fermentor, and inoculating to non-woven strips. Its advantages are high and durable effect on pests (such as longicorn), low cost and no environmental pollution.

The invention provides a solid culture medium and culture method of beauveria. The solid culture medium comprises vermiculite, wheat bran and corn flour in a mass ratio of 1:(1-1.2):(1-1.2), wherein vermiculite, wheat bran and corn flour are mixed and sterilized with steam for 40 minutes, thus obtaining the solid culture medium. The culture method comprises the steps of culturing beauveria inoculation fluid in a liquid enlarge culture medium to which gentamicin sulphate is added, then inoculating the beauveria inoculation fluid into the solid culture medium, and culturing the beauveria inoculation fluid at 25-27 DEG C for 7.5-8 days, thus harvesting beauveria. The solid culture medium has good moisture retention and breathability, can provide rich N and C source nutrients for beauveria proliferation, and is economical and accessible in raw materials. The culture method is simple to operate, is clear in flow, has low requirements for production environments and production equipment, is relatively low in production cost and relatively short in production period, dispenses with discharging the culture medium in production so as to completely eradicate the problem of pollution of mixed fungi in production, and is very suitable for small enterprises and production units to quickly produce beauveria.

The invention provides a method for producing plate cocoons and white muscardine silkworms simultaneously. The method mainly comprises the following steps: after silkworms are raised to fifth-instar silkworms, according to fungus strain implantation nodes, beauveria spores are implanted into silkworm bodies, when matured silkworms appear, mulberry leaves which are sprayed with a molting hormone are applied to feed the silkworms, when the silkworms spin and begin to have a little urination, the silkworms are laid on a spinning bed, the angle of the spinning bed is adjusted to make the silkworms crawl uniformly and spin, when silkworms close feet and fall, all silkworms are swept from the spinning bed, the swept silkworms are put into a bamboo basket uniformly, the plate cocoons are collected, the bamboo basket is put into a white muscardine silkworm producing room which has a temperature and humidity until muscardine silkworms are white, baking or drying in the sun is performed, and therefore the white muscardine silkworm finished products are obtained. According to the method, production of the plate cocoons and production of the white muscardine silkworms are combined, so that the yield of the plate cocoons can be guaranteed, economic benefits of producers are improved, and meanwhile the rigidification rate of the produced white muscardine silkworms is high, the cleanness degree of stiff silkworm is high, and drug efficacy meets requirements of Chinese pharmacopoeia.

The invention discloses a culture medium and method capable of increasing Beauveria brongniartii sporulation quantity favorably. The culture medium includes a solid culture medium and a liquid culture medium. The liquid culture medium comprises 3-5mg / l of glucose, 1-5mg / l of peptone and 1-5mg / l of yeast powder, wherein dissolving solvent is water. The solid culture medium comprises yellow mealworm dung, rice husk and rice, wherein the mass ratio of the yellow mealworm dung to the rice husk to the rice is 0.5:1:10. The culture method includes: evenly inoculating fungal spores into the liquid culture medium, placing into a 1000ml triangular flask, performing shake culture for 96 hours at 25 DEG C and 180r / min to obtain seed culture fluid, inoculating the seed culture fluid into the solid culture medium, placing the culture medium into a stainless steel tray and culturing for 3-10 days. The culture medium and method has the advantages that the waste yellow mealworm dung and rice husk are turned into wealth, culture cost is lowered, spore yield is increased, and production cycle is shortened.

The invention discloses a pesticide for preventing and treating tree long-horned beetles and a preparation method thereof and belongs to the technical field of biopesticide. The pesticide is prepared from, by weight, 0.5-10 parts of chitosan, 8-12 parts of garlic, 8-12 parts of croton, 6-10 parts of cortex cinnamomi, 5-10 parts of radix stemonae, 5-20 parts of plant essential oil, 3-6 parts of beauveria brongniartii, 4-10 parts of penicillium, 4-10 parts of bacillus thuringiensis, 5-12 parts of saccharomycetes and 50-200 parts of nutrient solution. The pesticide has an obvious prevention and treatment effect on the long-horned beetles, and is high in fatality rate and easy to prepare.

The invention belongs to the fermentation engineering technical field, in particular relates to the technics of producing swainsonine fermented by beauveria. The invention provides the technics of producing the swainsonine fermented by the beauveria to overcome the problems of the hard reproduction and the high cost of the prior art. In order to overcome the problems of the prior art, the technical scheme adopted by the invention is the process of producing the swainsonine fermented by the beauveria. The process successively includes the following steps: first, mycelium is obtainedthe through biological fermentation of the beauveria; second, the extraction of the swainsonine in the mycelium: firstly, the extraction of the total extract of the mycelium: the myceilium extraction concentrated solution is obtained by a ultrasonic method; secondly, the extraction of swainsonine crude products: the swainsonine crude products can be obtained by repeated extraction; thirdly, the extraction of the pure swainsonine: the pure swainsonine is obtained by the sublimation technology.

The invention belongs to the technical field of agriculture, and specifically relates to a beauveria brongniartii fungicide and application thereof. The beauveria brongniartii fungicide is prepared from beauveria brongniartii BRNS50206, a mixed adjuvant, a mineral carrier and a grain carrier, wherein the preservation number of the beauveria brongniartii BRNS50206 is CGMCC No. 15993. According to the beauveria brongniartii fungicide disclosed by the invention, beauveria brongniartii BRNS50206 is used as an active component of a fungicide, is specifically used for infecting insects but is safe to vertebrates, has no toxic and side effects, can be naturally blended with environment and is nondestructive and non-pollution, and the beauveria brongniartii fungicide is an environment-friendly andsafe biological pesticide. The beauveria brongniartii fungicide can be applied to preventing and treating injurious insects such as grubs, root weevil and cutworm of crops, forest, lawns and grass.

The invention discloses an insecticidal composition containing beauveria bassiana and diflubenzuron. The insecticidal composition is prepared from an effective component composed of beauveria bassianaand diflubenzuron, and an appropriate amount of an agricultural insecticide auxiliary agent is added. According to a preparation method, reasonable utilization of low dosage chemical pesticide diflubenzuron and beauveria bassiana spore germination promoter strigolactone is capable of improving the rapid effect and comprehensive prevention and treatment effect of beauveria bassiana on crop pests greatly, reducing using of chemical pesticides, reducing ecological destroy and environment pollution, and increasing crop yield and quality.

The combination of keratin hydrolysate with viable cells or infectious propagules of a fungal biological control agent such as Paecilomyces species, Metarhizium species, Beauveria species, and / or Verticillium species, provides effective control of soil-dwelling or subterranean insects, including termites, and particularly those belonging to the family Rhinotermitidae, such as the Formosan subterranean termite and native North American subterranean termites. In use, a foam-forming composition comprising the keratin hydrolysate and an insecticidally effective amount of the fungus are applied to the insects, the locus of the insects, to material susceptible to infestation by said insects, or to the locus of material susceptible to infestation by said insects.

The invention discloses a culture medium for beauveria brongniartii and a culture method. The culture medium is used for improving the sporulation quantity of the beauveria brongniartii and the virulence, to an insect, of the beauveria brongniartii. The culture medium is prepared from the following components with component contents: peanut shell, rice flour, wheat bran and eupolyphaga slough, which are in a mass ratio of (1 to 5) to (0.5 to 1.5) to (2 to 6) to (1 to 3). The culture method comprises the following steps of 1), preparation of seed liquid of a solid-phase fermentation culture medium for the beauveria brongniartii; 2), solid-phase fermentation culture; 3), inoculation and culture. The culture medium adopted by the invention can be used for reducing the culture cost, can be also used for increasing a spore yield, is used for shortening a production period, and further, can be used for improving the virulence, to the insect, of a spore.

The invention provides 2-coumaranone producing Beauveria, namely Beauveria tenella CGMCC NO.2382, and also provides a method for preparing 2-coumaranone by the Beauveria tenell. The method comprises the steps of preparing Beauveria fermentation liquid, centrifuging, extracting and drying the fermentation liquid to get crude extract dry powder, and carrying out separation by using atmospheric silica gel chromatography so as to get a substance containing the 2-coumaranone. The method gets the 2-coumaranone through microbial fermentation, which is simple in method and low in cost and causes no pollution during production, compared with the chemical synthesis method. The Beauveria tenella provided by the invention not only can be used for extracting the 2-coumaranone, but also can be used for preventing and controlling pine caterpillar damage.

The invention discloses a buffer solution for the normal-temperature preservation of recombinant beauveria brongniartii protease K and applications thereof. The buffer solution comprises the following components: trihydroxymethyl aminomethane Tris, a soluble sodium salt, a soluble calcium salt, glycerin, and the balance of water, wherein the pH value of the buffer solution is 7.5-8.4. Compared with the prior art, the buffer solution can significantly enhance the time of normal-temperature preservation of recombinant beauveria brongniartii protease K and does not degrade the catalytic efficiency of the recombinant beauveria brongniartii protease K; and the recombinant beauveria brongniartii protease K stored by using the buffer solution disclosed by the invention is directly applied without being processed, such as being applied to the extraction of nucleosides, and the like, so that the transportation, storage and application costs of the recombinant beauveria brongniartii protease K are significantly reduced, therefore, the buffer solution has a wide application prospect.

The invention provides isolation, identification and herbicidal activity research for Beauveria beauveria bassiana NO6, and relates to the technical field of microbial application. The Beauveria beauveria bassiana NO6 can generate significant inhibition effects on growth of Avena avena fatua in soil and exerts prevention and removal effects on the Avena avena fatua, thereby guaranteeing the growthquality and yield of planted crops; and compared with a pesticideherbicide for weeding, the Beauveria beauveria bassiana NO6 can avoid pesticideherbicide residues in crops, so the pollution to the soil and the environment is reduced. The invention provides a compound which contains a Beauveria beauveria bassiana NO6 preparation and is used for weeding; and the Beauveria beauveria bassiana NO6 preparation comprises a fermentation filtrate and / or a crude extract. According to a technical scheme of the invention, the Beauveria beauveria bassiana NO6 is applicable to the processes of prevention and removal of the Avena avena fatua.

The invention discloses a non-chemical distiller's yeast pest control method and a three-stage distiller's yeast storage method based on the non-chemical distiller's yeast pest control method. The invention is characterized in that the control method is composed of the following approaches: distiller's yeast warehouse oxygen content is reduced through controlled atmosphere, such that the reproduction rate and adult rate of the distiller's yeast pests are inhibited; the distiller's yeast pests are killed with beauveria and diatomaceous earth; and the distiller's yeast pests are killed with trapping lamps. During a distiller's yeast storage period, with a combination of the above control approaches, adult pest number is gradually reduced through three stages. Distiller's yeast pest control is carried out according to actual situations of different stages of the distiller's yeast. With the distiller's yeast pest control and distiller's yeast storage methods provided by the invention, distiller's yeast pests can be effectively killed, distiller's yeast storage quality can be improved, white spirit loss can be reduced, and production efficiency can be improved.

The invention discloses a microbial pesticide for growth stage of wheat. The microbial pesticide comprises the following components in parts by weight: 3-5 parts of beauveria brongniartii, 3-5 parts of green muscardine fungus, 9-13 parts of paecilomyces varioti, 4-6 parts of verticillium, 3-4 parts of 2-methyl-4-chloro-isooctyl ester, 1-6 parts of fluopyram, 5-8 parts of difenoconazole, 26-29 parts of ammonium dihydrogen phosphate, 6-8 parts of rhizobium powder, 8-9 parts of gelsemium elegan, 1-3 parts of Chinese azalea flower, 1-3 parts of common threewingnut roots, 1-6 parts of strychnos ignatia, 12-18 parts of an emulgator, 19-22 parts of a synergist, 5-8 parts of dandelion, 5-9 parts of pomegranate peel, 1-4 parts of luffa stems, 1-4 parts of trifoliate oranges, 3-4 parts of radix stemonae, 9-11 parts of camphor and 60-90 parts of water. The microbial pesticide for growth stage of wheat provided by the invention has the advantage of being a novel and nuisanceless pesticide which is efficient, targeted and environment-friendly, avoids secondary toxicity, residues and pollution, and keeps ecological balance.

The invention discloses a recombinant beauveria brongniartii proteinase K as well as an industrial production and purification method thereof and relates to the technical field of gene modification and protein engineering. Recombinant beauveria brongniartii proteinase K coding genes suitable for the pichia pastoris recombinant expression are obtained with a biological information means, optimization genes are synthesized and successfully expressed in a eukaryotic system, and the recombinant proteinase K with the enzymatic activity obviously higher than that of a traditional tritirachium album limber proteinase K. According to the recombinant beauveria brongniartii proteinase K as well as the industrial production and purification method thereof, the coding genes are synthesized chemically, and GS115 / pPIC9K-ProK high-yield engineering bacteria are established, so that defects of the low yield and the high cost of natural products are overcome, the usage cost of the proteinase K in related industries is significantly reduced, and the recombinant beauveria brongniartii proteinase K as well as the industrial production and purification method thereof have broad application prospects.

The invention belongs to the field of planting, and particularly relates to a method for controlling tea pests and diseases by using Beauveria. The method mainly comprises the following steps: preparation of a preparation, field application, inspection of control results, and the like. A medicament for preventing and controlling tea pests and diseases disclosed by the invention has good control effects on pests and diseases, and has 90% or more control effect on Jacobiasca formosana, tea aphids, tea caterpillars and mites, and also has 90% or more control effect on pests of diseases. The preparation disclosed by the invention has the advantages of wide source of materials, simple composition and no pesticide residue, and is a green and high-efficiency tea pest and disease control preparation.

The invention provides application for controlling asparagus prodenia litura by utilizing beauveria bassiana. The application specifically comprises the step of applying an active beauveria bassiana product into an asparagus field; a preparation method of the active beauveria bassiana comprises the following steps of separating and purifying the beauveria bassian from prodenia litura in which the beauveria bassiana parasitizes; performing expanding culture on the separated and purified beauveria bassiana, and preparing into an active beauveria bassiana product. According to the application of the beauveria bassiana in control of the asparagus prodenia litura, by utilizing the effects of high infection, parasitism, lethal and re-propagation of the beauveria bassiana to paragus prodenia litura, the paragus prodenia litura is prevented and controlled, and aims of reduction of the use of chemical pesticide, control of environmental pollution, realization of environment friendly production are achieved.

The invention belongs to the technical field of agricultural biology, and particularly relates to Beauveria brongniartii BRNS50206 and an application thereof. The Beauveria brongniartii BRNS50206 is taken as a biological insecticide, has high toxicity on target pests, can realize good control effect and facilitates reduction of use of chemical pesticides in plant protection, and meanwhile, a strain is non-toxic and harmless to people and livestock, is environmentally friendly and pollution-free and guarantees crop safety and ecological safety.

The present invention relates to a composition with whitening, anti-aging, repairing and anti-sensitivity functions containing mulberry extract, including mulberry extract, milk, egg white, honey, Atractylodes macrocephala, Angelica dahurica, Bletilla striata, Poria, Radix Paeoniae Alba, Poria cocos, White silkworm, pearl powder, white monkshood, white wax, green woody fragrance, Chuanqiong, almond, ginkgo, asparagus, nard pine and sheep marrow, among which, Atractylodes macrocephala, Angelica dahurica, Bletilla striata, Baishui, white peony root, white poria cocos, white jellyfish 60 grams of silkworm, pearl powder, white monkshood, white wax, green wood fragrance, and Chuanqiong, 30 grams of almonds, ginkgo, asparagus, and nard pine, 750 grams of sheep marrow, 100 grams of milk, 15 grams of egg white, and 20 grams of honey. Mix and crush the above medicines, soak in 250ml of wine and water for 12-18 hours, then simmer for 6-8 hours until the wine evaporates, filter off the residue, and store in a cool place with porcelain.