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White muscardine fungi and method for preparing 2-coumaran ketone

A technology of coumarone and Beauveria bassiana, which is applied in the field of Beauveria bassiana, can solve the problems of high cost, environmental pollution by by-products, and low yield, and achieve the effect of low cost, no pollution, and simple method

Inactive Publication Date: 2008-11-12
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

2-Coumarone is currently mainly obtained through chemical synthesis, which not only has high cost and low yield, but also pollutes the environment by-products produced during the production process

Method used

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  • White muscardine fungi and method for preparing 2-coumaran ketone
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  • White muscardine fungi and method for preparing 2-coumaran ketone

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Experimental program
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Effect test

Embodiment 1

[0022] The preparation of 2-coumarone comprises the steps:

[0023] (1) Insert the Beauveria bassiana oosporium strain with the preservation number CGMCC NO.2382 into the slant culture medium, and cultivate it at a temperature of 25° C. and a relative humidity of 75% for 7 days to obtain the slant strain;

[0024] (2) Insert the slant strains under aseptic conditions into a 500ml Erlenmeyer flask equipped with 200ml liquid medium, place in a constant temperature shaking incubator, 180r / min at 25°C, and cultivate for 7 days to obtain a seed solution;

[0025] (3) In a 7L liquid fermenter, put 5L of liquid medium into it, put 1 bottle of the above-mentioned cultured seed solution into the fermenter, and cultivate it at 25±0.5°C and 375±5r / min for 7 days to obtain Bacteria fermentation broth;

[0026] The proportion of the solid slant medium: peptone 10g / L, yeast extract 10g / L, glucose 40g / L, agar 20g / L, distilled water to volume; the liquid medium proportion: peptone 10g / L, yea...

Embodiment 2

[0035] Test on Insecticidal Activity of Beauveria tenella CGMCC NO.2382

[0036] PDA medium: potato 200g, agar 20g, glucose 20g, distilled water 1000ml.

[0037] The tested insects were 4-5 instar healthy larvae of Pinus tabulaeformis collected from the Forest Farm of Xishan Mineral Bureau, Lingqiu County, Shanxi Province.

[0038] Preparation of spore suspension: After cultivating the test strains on a slant, use 0.1% Tween 80 sterile water to prepare a concentration of 1.0×10 7 spore suspension per ml.

[0039] Insecticidal Activity Test of Beauveria bassiana Spore Suspension on Chinese Pine Caterpillar

[0040] With a concentration of 1.0 x 10 7The 5th instar larvae of Chinese pine caterpillars were infected by the spore suspension per ml, 30 in each group, and 3 repetitions and controls were established. The 5th instar larvae of Chinese pine caterpillar infected with the spore suspension and the blank control group were observed. Infection group: The body weight incre...

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Abstract

The invention provides 2-coumaranone producing Beauveria, namely Beauveria tenella CGMCC NO.2382, and also provides a method for preparing 2-coumaranone by the Beauveria tenell. The method comprises the steps of preparing Beauveria fermentation liquid, centrifuging, extracting and drying the fermentation liquid to get crude extract dry powder, and carrying out separation by using atmospheric silica gel chromatography so as to get a substance containing the 2-coumaranone. The method gets the 2-coumaranone through microbial fermentation, which is simple in method and low in cost and causes no pollution during production, compared with the chemical synthesis method. The Beauveria tenella provided by the invention not only can be used for extracting the 2-coumaranone, but also can be used for preventing and controlling pine caterpillar damage.

Description

Technical field: [0001] The invention relates to Beauveria bassiana, in particular to a Beauveria bassiana and a method for preparing 2-coumarone. Background technique: [0002] 2-coumaranone (2-coumaranone), also known as n-hydroxyphenylacetic acid lactone, is a raw material for the synthesis of insecticides and physiological reagents. 2-Coumarone is currently mainly obtained through chemical synthesis, which not only has high cost and low yield, but also pollutes the environment by-products produced during the production process. If the method of producing 2-coumarinone by microbial fermentation is likely to solve the above problems. Invention content: [0003] The object of the present invention is to provide a Beauveria bassiana producing 2-coumarinone. [0004] Another object of the present invention is to provide a method for preparing 2-coumarinone by using the strain. [0005] A Beauveria tenella producing 2-coumarinone provided by the present invention is Beauv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/62A01P7/04C12R1/645
Inventor 谢映平樊金华薛皎亮李保珍
Owner SHANXI UNIV
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