20 results about "Pinus tabulaeformis" patented technology

The application relates to the technical field of gene chips, in particular to a SNP molecular marker combination of Pinus massoniana and application thereof. The SNP molecular marker combination is composed of 113,709 SNP molecular markers, wherein the physical position of the SNP molecular markers is determined by sequence alignment based on a reference genome Pinus.tabuliformis V1.0 of Pinus tabulaeformis. The application provides a whole genome chip of Pinus massoniana containing 113,709 SNP sites for the first time, and the chip has the advantages of high efficiency, low cost, good genetic stability and the like, and can be widely applied to different application scenarios such as identification of Pinus massoniana germplasm resources, genetic background analysis of breeding materials, gene positioning, whole genome association analysis, whole genome selection breeding and intelligent design breeding.

The application discloses a protein separated from Pinus tabulaeformis Carr. Pinus tabuliformis ​ and an encoding gene PtAMT2.8 of the protein, which can significantly enhance nitrogen absorption and transportation of plants. 31019b The PtAMT2.8 gene is introduced into a yeast mutant strain with a deficiency in ammonium nitrogen absorption function, so that the growth phenotype of the yeast mutant is recovered, and the PtAMT2.8 gene is introduced into Pinus tabulaeformis and Arabidopsis thaliana, so that the nitrogen absorption and transportation capacity of the transgenic plants can be significantly enhanced. The application has important significance for accelerating genetic improvement of plants and cultivating new varieties with high yield, high quality and multi-resistance.

The invention discloses a protein which is separated from pinus tabuliformis and is capable of remarkably enhancing nitrogen absorption and transport of a plant, and a coding gene PtAMT2.8 of the protein. The PtAMT2.8 gene is introduced into a yeast ammonium nitrogen absorption function deficient mutant strain 31019b, so that the growth phenotype of a yeast mutant can be recovered, and the nitrogen absorption and transport capability of a transgenic plant can also be remarkably enhanced by introducing the PtAMT2.8 gene into pinus tabulaeformis and a model plant arabidopsis thaliana. The invention has important significance in accelerating plant genetic improvement and cultivating high-yield, high-quality and multi-resistant new varieties.

The utility model provides a kind of for Pinus taeda growth environment monitoring device, belong to soil monitoring equipment technical field, the device includes support plate, fixed monitoring mechanism, fixed block, monitoring cabinet, water tank, water inlet bucket and apron, in use, monitoring probe is connected in fixed clamping plate, data transmission line is set in slot simultaneously, then insert pipe is inserted into land, monitoring probe is sent into ground bottom by insert pipe, to carry out real-time monitoring to underground soil environment, insert pipe can fix the whole device simultaneously, it is convenient to fix installation monitoring device on sand, further improve the stability of the whole device fixed by cooperation support plate and support leg, in addition, rainwater can enter water inlet bucket by multiple water inlets on apron, rainwater can be collected and stored by water tank, it is convenient to carry out irrigation work, meanwhile, water inlet bucket, connecting pipe and apron can reduce the transpiration loss of water under high temperature environment.

The invention discloses application of a Pt7G25060 gene and a protein thereof in judging drought stress degree of Chinese pine seedlings, and belongs to the technical field of biology. The expression quantity of the gene is in negative correlation with the drought stress degree, and obvious expression difference is shown in the early stage of drought stress of Chinese pine seedlings. Based on the technical advantages, the invention provides the method for judging the drought stress degree of the Chinese pine seedlings. The cDNA of a sample to be detected and the cDNA of a reference sample are subjected to fluorescent quantitative PCR amplification by using the detection primer provided by the invention, and the expression quantity of the Pt7G25060 gene in the sample to be detected and the expression quantity of the Pt7G25060 gene in the reference sample are compared, so that the drought stress degree of the sample to be detected can be accurately judged. Compared with a traditional distinguishing method depending on observation of seedling needle leaf sallow degree and physiological indexes, the method provided by the invention can sensitively and accurately reflect the drought stress degree of the Chinese pine seedlings, and is higher in precision, stronger in scientificity and good in repeatability.

This invention provides a method for constructing a characteristic spectrum of Pinus tabuliformis granules, the characteristic spectrum, and its application. The construction method includes the following steps: (1) preparing a test solution of Pinus tabuliformis granules; preparing a reference solution of Pinus tabuliformis reference material; and preparing a reference solution of pine syrup monomethyl ether reference standard; (2) performing ultra-high performance liquid chromatography (UHPLC) on the test solution, the reference solution of the reference material, and the reference solution of the reference standard, respectively, and obtaining the characteristic spectrum of the Pinus tabuliformis granules based on the detection. The characteristic spectrum constructed by the method provided by this invention enhances the specific identification of Pinus tabuliformis granules and can effectively identify the source of Pinus tabuliformis medicinal material, providing support for ensuring the single source of the medicinal material source of Pinus tabuliformis granules. The characteristic spectrum constructed by this invention can comprehensively reflect the characteristic peak information of the sample, and the method is stable, highly precise, and has good reproducibility.

The invention discloses a specific probe combination for pinus tabulaeformis resistant bursaphelenchus xylophilus character genotyping, a liquid phase capture chip containing the probe combination, application of the probe combination or the chip, and a method for detecting a biological sample by using the probe combination or the chip. The specific probe combination is used for pinus tabulaeformis resistant bursaphelenchus xylophilus character genotyping of the pinus tabulaeformis resistant bursaphelenchus xylophilus resistant to the bursaphelenchus xylophilus resistant to the bursaphelenchus xylophilus resistant to the bursaphelenchus xylophilus resistant to the bursaphelenchus xylophilus.

The invention discloses an injection device for precisely applying pesticide to root diseases of Chinese pines, which comprises a placing frame, auxiliary discs are arranged at the upper end of the placing frame, a positioning pile is fixedly connected to the lower end of the placing frame, one end of the positioning pile is fixedly connected with a limiting plate, the number of the auxiliary discs is two, and the two auxiliary discs are symmetrically distributed; a movable bin is formed in the inner side of the auxiliary disc; an operation channel is provided for a soil breaking drill and a hole taking drill through auxiliary holes formed in an inclined panel, the auxiliary holes are used for breaking soil, conveying soil and drilling holes, meanwhile, the guiding and auxiliary stabilizing effects are achieved in the follow-up injection process, and by means of cooperation of an auxiliary plate and a clamping piece and cooperation of a positioning cylinder and a cross-shaped clamping block and a cross-shaped notch in a pressing injector, the pressing injector can be conveniently and rapidly pressed; the pressing injector is effectively prevented from deviating and misplacing in the using process, the injection accuracy is ensured, liquid medicine can accurately act on the disease portion of the root of the Chinese pine, and the treatment effect is improved.

This invention discloses a traditional Chinese medicine composition for reducing swelling and relieving pain, and its preparation method. The composition comprises the following medicinal ingredients: *Lycopodium clavatum*, *Clematis chinensis*, safflower, ephedra, *Pinus tabuliformis*, *Liquidambar formosana*, *Millettia speciosa*, *Tinospora sinensis*, *Caesalpinia sappan*, *Sparganium stoloniferum* (processed with vinegar), *Curcuma zedoaria*, and *Homalomena occulta*. All ingredients in this composition are natural Chinese herbal medicines, making the raw materials readily available, the preparation method simple, the cost low, and the use convenient. It can be mass-produced. This composition can effectively relieve symptoms of swelling and pain, improve the quality of life, and has high social value.

The application provides a method for grafting Pinus tabulaeformis with Pinus yunnanensis var. biondii, and belongs to the technical field of grafting of needle-leaved trees, and solves the technical problems of slow grafting, high pathogenic infection rate and low survival rate of less than 40% caused by environmental fluctuations. The technical solution points comprise the following steps: cutting a vertical incision of 1.8-2.2 cm deep to the xylem at a position of 2.5-3.5 cm from the surface of the cultivation substrate exposed at the root stem of the stock; immersing the base of the scion into a sodium citrate buffer solution containing polyvinylpyrrolidone, methyl salicylate and calcium chloride after the base is cut into a symmetrical double-wedge shape; coating adsorption glue on both sides of the vertical incision; inserting the scion to align the cambium and fixing the scion with a grafting tape; applying an aqueous ethanol solution containing salicylic acid, trehalose and polysorbate 80 to the grafting interface every 2-3 days; maintaining the day temperature at 23-25 DEG C, the night temperature at 16-18 DEG C and the humidity at 90-95%; girdling the stock when the new shoots grow to 2-3 cm in length, and cutting off the main stem after 7-10 days. The application is used for large-scale propagation of Pinus yunnanensis var. biondii grafted seedlings.

The application discloses two strains of endophytic bacillus and application thereof in promoting plant growth and resisting blue-rot disease, and belongs to the field of plant growth promoting bacteria. The two strains of endophytic bacillus are isolated from tissue samples of healthy Pinus tabulaeformis and identified as bacillus, and the microbial preservation numbers thereof are CGMCC No. 37659 and CGMCC No. 37660 respectively. The two strains of bacillus both show strong inhibitory activity to a pathogenic fungus of blue-rot disease and can produce siderophores; one of the strains has the ability to produce IAA and does not have chitinase activity; the other strain does not produce IAA but produces chitinase; the two strains of bacillus have both commonness and complementarity in function, and the combination of the two strains can effectively promote the early germination of Pinus tabulaeformis seeds, significantly increase the biomass of Pinus tabulaeformis and significantly improve the resistance of the host plant to blue-rot fungus L. procerum , and has a significant prevention and treatment effect on the blue-rot disease of the plant caused by the blue-rot fungus.

The application provides a Pseudomonas chlororaphis strain Rhs-P27 in the application of preventing and treating pine wood nematode disease, relates to the field of agricultural environmental microorganism technology. Specifically relates to a Pseudomonas chlororaphis strain Rhs-P27 isolated from the rhizosphere soil of Pinus tabulaeformis and the application of the strain in the prevention and treatment of pine wood nematode disease. The strain is preserved in China Center for Type Culture Collection of Wuhan University on December 18, 2024, and the preservation number is CCTCCM20242846. The in vitro toxic killing activity of the strain Rhs-P27 to pine wood nematode can reach 100% in 24 hours. The fermentation filtrate and fermentation bacteria suspension of the strain Rhs-P27 have significant inhibitory effect on the reproduction of pine wood nematode. The prevention effect of the fermentation bacteria suspension of the strain Rhs-P27 on the pine wood nematode disease of two-year-old Pinus tabulaeformis pot seedlings can reach 45%, and the density of pine wood nematode in the pine tree can be significantly reduced. The Pseudomonas chlororaphis strain Rhs-P27 of the application has a good application prospect in the field of nuisance-free prevention and treatment of pine wood nematode disease.

The application belongs to the technical field of plant genetic engineering, and particularly relates to application of a marker gene PtAP2R1 in identifying vegetative growth stages and reproductive growth stages of Pinus tabulaeformis. The marker gene PtAP2R1 is expressed in large amounts in the vegetative growth stage of Pinus tabulaeformis, and is expressed in small amounts in the reproductive growth stage of Pinus tabulaeformis, and then is continuously expressed at a low level, and the expression amount of the marker gene PtAP2R1 shows a very significant difference between the vegetative growth stage and the reproductive growth stage of Pinus tabulaeformis, and the growth and development stage of Pinus tabulaeformis can be identified according to the expression amount of the marker gene PtAP2R1. Further, the application can efficiently and accurately identify the growth and development stage (vegetative growth and reproductive growth) of Pinus tabulaeformis plants or even selected branches by comparing the expression amount of the marker gene PtAP2R1 in a to-be-tested sample of Pinus tabulaeformis with the expression amount of the marker gene PtAP2R1 in a sample of Pinus tabulaeformis in a known growth stage, thereby providing guidance for forestry production and forest management.

The invention belongs to the technical field of environmental microorganisms and ecological restoration, and provides a barren-resistant acinetobacter, an agrobacterium tumefaciens, a complex microbial inoculant thereof and application of the complex microbial inoculant in promoting vegetation restoration of pinus tabulaeformis in mining areas, the preservation number of the barren-resistant acinetobacter YSR-01 is CGMCC No.35513, the preservation number of the agrobacterium tumefaciens YSP-02 is CGMCC No.35515, and the preservation dates of the acinetobacter and the agrobacterium tumefaciens are both August 4, 2025. The two strains are compounded to prepare the complex microbial inoculant. The microbial agent can effectively improve the nutrient condition of barren soil in a mining area through the synergistic effect of potassium and phosphorus dissolving, and growth of host plants (such as Chinese pines) is remarkably promoted due to the excellent barren resistance and colonization capacity of the microbial agent. Experiments prove that the growth promoting effect of mixed application of the two strains is obviously better than that of single application, and the method has important value for accelerating ecological reconstruction of mining areas.

This invention provides a method for isolating cell nuclei from *Pinus tabuliformis* tissue, relating to the field of plant molecular and cell biology. The method uses *Pinus tabuliformis* callus tissue as explants, releases protoplasts using a specific enzymatic hydrolysis solution, lyses them using a specific lysis buffer, and finally filters and centrifuges to obtain *Pinus tabuliformis* cell nuclei. Using this invention, cell nuclei suitable for single-cell and histone modification library construction and sequencing can be rapidly isolated, providing a foundation for research on single-cell nuclear genetic material transcription analysis, tissue-specific analysis, and cell fate determination in gymnosperms, and offering research ideas for the future use of cell nuclei in coniferous genetic engineering.

This invention provides a method, system, computer equipment, and medium for detecting the health status of Pinus tabuliformis, belonging to the field of forestry informatization. The method includes acquiring image data of the Pinus tabuliformis to be detected; collecting feature maps containing global spatial information of the Pinus tabuliformis from the image data; performing local channel interactions on the corresponding channel descriptors of the feature maps to obtain local channel information; capturing the dependencies between channels of the channel descriptors to obtain global information; weighting different channels in the feature maps based on the local and global information to obtain context-attention-weighted feature maps; fusing enhanced features at multiple scales to obtain fused features; and determining the health status detection result of the Pinus tabuliformis to be detected based on the fused features. This method strengthens local features representing subtle changes, establishes a correlation matrix between local and global information, and generates weights from this matrix, thereby selectively weighting different channels of the feature maps and improving the accuracy of Pinus tabuliformis health status detection.

The utility model discloses a double-matrix structure planting layer planting system for tree pool Chinese pines, which comprises a double-matrix structure planting layer and a lower water vapor supplementing system, and the double-matrix structure planting layer sequentially comprises a soil matrix layer, a non-woven fabric isolating layer, a draining matrix filling layer and a ventilation water collecting pipe from top to bottom, the ventilation water collecting pipes are made of PVC materials and laid at the bottom of the draining matrix filling layer in the horizontal direction. The ecological function of soil can be remarkably improved, and the water-gas exchange condition can be optimized.

This invention discloses a traditional Chinese medicine composition for treating and improving knee osteoarthritis complicated with sarcopenia and its application, belonging to the field of traditional Chinese medicine compositions. The composition comprises 6-15 parts of Angelica pubescens, 10-20 parts of Achyranthes bidentata, 10-20 parts of Eucommia ulmoides, 10-20 parts of Dipsacus asper, 6-15 parts of Psoralea corylifolia, 6-12 parts of Citrus aurantium, 6-12 parts of Platycodon grandiflorus, 3-10 parts of Cimicifuga foetida, 10-20 parts of Atractylodes macrocephala, and 10-30 parts of Pinus tabuliformis. The preparation method includes mixing the raw materials, adding water, and decocting to extract the decoction; it can also be further prepared into granules, capsules, or tablets. Animal experiments and clinical trials have confirmed that the traditional Chinese medicine composition of this invention can relieve joint pain, improve lower limb muscle strength and mobility, increase skeletal muscle mass, and reduce inflammatory factor levels, achieving dual intervention of joints and muscles.

The invention discloses a probe combination for gene typing of drought resistance characters of Pinus tabuliformis, a chip comprising the probe combination, application of the probe combination or the chip, and a method for detecting a biological sample by using the probe combination or the chip.

The invention belongs to the technical field of biological prevention and control of forestry diseases, and discloses a microorganism for inhibiting chaetosporum oleolatum, which is Erwinia separated from leaf rhizosphere of pinus tabulaeformis, is preserved in China General Microbiological Culture Collection Center (CGMCC), and has a preservation number of CGMCC NO.36570. The invention further discloses a preparation method of the microorganism for inhibiting chaetosporum oleolaeformis. The screened microorganism for inhibiting the chamomile oil is high in environmental adaptability, excellent in antibacterial effect on the chamomile oil and high in host adaptability, does not cause harm to non-target organisms in Chinese pines and forest ecosystems, and can also avoid residual pollution of traditional chemical agents. On the basis of the microorganism which is separated, purified and cultured, the microorganism can be further prepared into fungicide such as wettable powder and a suspending agent, can be used for field prevention and treatment of pinus tabulaeformis needle drop disease, and can also be used as a core strain of a composite biocontrol fungicide, so that the application scene is expanded.