4 results about "Xylophilus" patented technology

The application discloses a composition for preventing and treating Bursaphelenchus xylophilus and application thereof, and belongs to the technical field of biological pesticides. The composition is compounded by taking a crude extract of Zanthoxylum bungeanum as a main active ingredient and a crude extract of Astragalus membranaceus as a synergistic ingredient. The composition has high Bursaphelenchus xylophilus killing activity. After Bursaphelenchus xylophilus is treated by 600ug / mL of the composition solution for 24 hours, the corrected mortality of the Bursaphelenchus xylophilus reaches 99.33%. The composition can effectively prevent the occurrence of the pine wilt disease in a forest, and the control effect reaches 79.59%. In addition, the preparation process of the composition is simple, the composition is easy to use, and the composition is suitable for popularization and use.

Provided are Lagenidium juracyae with a Bursaphelenchus xylophilus (B. xylophilus)-killing activity and a use of the Lagenidium juracyae in control of B. xylophilus. The Lagenidium juracyae has a strain number of NL03 and an accession number of CGMCC NO. 40587. In the present application, an oomycete Lagenidium sp. NL03 with a B. xylophilus-killing ability is isolated from dead soil nematodes. The biological and molecular identification and the biocontrol potential investigation are conducted for Lagenidium sp. NL03 to provide a reference for the expansion of a biological control resource pool for B. xylophilus and the eco-friendly control of pine wilt disease (PWD).

The invention relates to a composite bursaphelenchus xylophilus epidemic wood treatment bag and a bursaphelenchus xylophilus epidemic wood on-site disinfestation treatment method.The film body of the composite bursaphelenchus xylophilus epidemic wood treatment bag is of a five-layer composite structure, and the five-layer composite structure sequentially comprises an aluminum film layer, a first PE film layer, a polypropylene woven cloth film layer, a second PE film layer and a third PE film layer from outside to inside; the thickness of the aluminum film layer is 0.02 mm, the thickness of the polypropylene woven cloth film layer is 0.05 mm, the warp and weft density is 120 * 120 pieces per inch, and each piece of warp and weft is a rectangular flat filament; the thickness of each of the first PE film layer, the second PE film layer and the third PE film layer is 0.02 mm, the surface of the first PE film layer is subjected to corona treatment, the surface tension is larger than or equal to 38 mN / m, the third PE film layer is a sealed contact layer, the composite epidemic wood treatment bag and the treatment method can achieve full-scene on-site efficient pest removal of pine wood nematode disease epidemic wood, the prevention and control cost is reduced, and the prevention and control effect is improved.

The present application belongs to the field of biotechnology, and particularly relates to a chimeric dsRNA for preventing and treating pine wood nematode, a complex and application thereof. Let-526 (200 bp), EF1 (200 bp) and flp-1 (330 bp) gene fragments. The present application also provides a complex formed by delivering the chimeric dsRNA by using a chitosan nanocarrier, and a method for preventing and treating pine wood nematode by injecting the complex into a trunk. The chimeric dsRNA can simultaneously silence three key target genes, produce a synergistic lethal effect, make the mortality of pine wood nematode reach 88.8% in a pot experiment, and reduce the needle yellowing rate of a diseased black pine from 100% to 23.5%. Thus, the present application provides a new green prevention and control technology for pine wood nematode which is efficient, specific and environment-friendly.