38 results about "Xylophilus" patented technology

The present invention discloses a preparation method of a nitrogen doping carbonized bacterial cellulose/graphene/platinum composite nanomaterial. The material is prepared according to the following steps of pre-treating flocculent bacterial cellulose generated in fermentation of acetobacter xylophilus, then refrigering and drying the flocculent bacterial cellulose so as to obtain flocculent bacterial cellulose; homogenizing at high speed so as to obtain a uniform solution, mixing the uniform solution with graphene oxide, and dispersing the mixture uniformly through ultrasonic; and adding a dopamine solution, stirring and mixing by using a machine, adding chloroplatinic acid finally, and carbonizing at high temperature so as to obtain the nitrogen doping carbonized bacterial cellulose/graphene/platinum composite nanomaterial. The composite nanomaterial prepared by the method uses a one-step method to carbonize, and the reaction process is easy. Under the action of the dopamine, the flocculent bacterial cellulose and the graphene oxide are bonded, so as to form a three-dimensional networked cross-linked skeleton structure. In carbonization, nitrogen doping and platinum reduction are realized, and the conductive nanometer composite system that has small and uniformly distributed particles is prepared. The nanomaterial prepared by the method can be applied to application fields such as fuel batteries and super capacitors.

The invention discloses a technology for artificial domestication and breeding of sclerodermus and applied to prevention and treatment of pine sawyer beetles. The technology for artificial domestication and breeding of sclerodermus comprises the following steps of: firstly, feeding the sclerodermus to be substituted for the host of a barley pest; secondly, carrying out the selecting and refrigerating treatment of female sclerodermus; thirdly, packing the initiated pupa barley pest and the female sclerodermus into a finger-shaped tube, and plugging the tube with an ordered pine plug; and finally, neatly stacking and placing the tubes into a weather cultivation box with a cultivation temperature and conditions as well as prompts. In a bursaphelench xylophilus epidemic area, the throwing technology of sclerodermus comprises the following steps of: firstly, setting bait woods to induce the pine sawyer beetles to lay eggs; then examining the parasitic situation of the pine sawyer beetles in a pine tree section in an appropriate weather condition, and releasing the sclerodermus according to the groove number of the sawyer beetles; and finally investigating the parasitic situation of the sclerodermus. By the adoption of sawyer pests to prevent and treat the pine sawyer beetles so as to inhibit the occurrence of a bursaphelench xylophilus disease, the environment can not be polluted, the ecological balance is maintained, the biological diversity is kept, and the technology is an advanced technology for controlling the pine sawyer beetles and the bursaphelench xylophilus disease.

The invention discloses a bacillus pumilus JK-SD001. Subclass thereof is named as Bacilluspumilus and is preserved in China Center for Type Culture Collection CCTCC, preservation number thereof is M 209095, and preservation date is May 4th, 2009. The invention also discloses application of bacillus pumilus for killing bursaphelenchus xylophilus with poison. The invention also provides a micro organism bursaphelenchus xylophilus killing agent, obtained by the way that JK-SD001 is inoculated to a culture medium to be fermented and cultured and filtering is carried out by virtue of a filter with the aperture of 0.22Mum. The micro organism bursaphelenchus xylophilus killing agent of the invention has higher toxicity on bursaphelenchus xylophilus (B. xylophilus), and culture condition is simple and preservation is easy, thus being easy for industrialized production and having good application prospect.

The invention provides a pesticide for preventing and controlling Bursaphelenchus xylophilus and an injection method thereof. The pesticide for preventing and controlling Bursaphelenchus xylophilus comprises an effective medicament X, compound auxiliaries Ai-Aj and solvent S which form an X-Ai-Aj-S system; based on preparing 1L of the pesticide, the consumption of the compositions is that: the consumption of the effective medicament X is 5 to 40g, the consumption of the auxiliary Ai is 100 to 150ml, the consumption of the auxiliary Aj is 150 to 200ml, the consumption of the solvent S is 600 to700ml, and the balance is water; and the concentration of the prepared pesticide is 0.5-4g/ml. Through experiments, the pesticide has good effect of preventing and controlling the Bursaphelenchus xylophilus, and also has good effect of preventing and controlling Monochamus alternatus Hope, Atysa marginata cinnamomi Chen, sweetgum palmerworms, Dendrolimus houi Lajonquiere, Hylobius abietis, pine bark beetles and the like; and drilling machinery provided by the invention and the injection method adopting the drilling machinery are simple and practical, do not consume fuel or electricity, and are particularly suitable to meet the requirement of preventing and controlling plant diseases and insect pests in mountain woods and forests.

The invention discloses a method by the adoption of biological control. According to the method, the populations of the monochamus alternatus of pine forests are obviously reduced and controlled, and the number of pines which die from bursaphelenchus xylophilus is also obviously reduced. Drone pupae of bombus hypocrita are adopted as hosts of sclerodermus sp., meanwhile proliferation conditions are regulated and controlled, and therefore the parasitic ability of the sclerodermus sp. obtained through proliferation is remarkably improved.

The invention discloses protein BxCDP1 of bursaphelenchus-xylophilus pathogen-associated molecular patterns and application thereof. The amino acid sequence of the protein BxCDP1 of the pathogen-associated molecular patterns (PAMPS) is shown in SEQID NO.2. From effectors secreted by bursaphelenchus xylophilus and the PAMP, the defensive responses of a host plant, namely, a pine tree, to bursaphelenchus xylophilus invasion are studied, the protein BxCDP1 of the pathogen-associated molecular patterns is obtained from the bursaphelenchus xylophilus, it is proven through experiments that the protein BxCDP1 can trigger cell necrosis of various plants including the host plant, has a certain broad spectrum in cell necrosis triggering, and stimulates the defensive response of the host plant. The BxCDP1 triggered cell necrosis depends on a co-receptor BAK1 of pattern recognition receptors, the BxCDP1 can stimulate the accumulation of nicotiana benthamiana ROS and up regulation of PTI Marker genes, and the immunoreaction of the nicotiana benthamiana is stimulated. It can be seen that the BxCDP1 is a PAMP secreted by the bursaphelenchus xylophilus, and has important theoretical and practicalsignificance for revealing the pathogenic mechanism of the bursaphelenchus xylophilus and improving the resistance of the pine tree to the bursaphelenchus xylophilus in a targeted mode.

The invention discloses a preparation method for a trunk injecting microcapsule pesticide for controlling Bursaphelenchus xylophilus and aims to prepare a pesticide capable of controlling occurrence of the Bursaphelenchus xylophilus. A microcapsule technology is adopted to compound a chitinase inhibitor pesticide with an abamectin biopesticide, and the preparation method comprises the specific flow as follows: (1) the chitinase inhibitor pesticide is ground with diesel oil as an assistant, and ointment is prepared; (2) the biopesticide is dissolved with a solvent and mixed with the ointment, and the mixture is stirred uniformly; a synergist, a surfactant and water are added, the materials are heated and stirred to be emulsified and dispersed, and an emulsion is prepared; (3) a wall material aqueous solution is added to the emulsion and stirred at the constant temperature of 55-65 DEG C, a microcapsule forming reaction is performed, a product is cooled after the reaction, and the trunk injecting microcapsule pesticide is prepared. The pesticide prepared with the method can kill the Bursaphelenchus xylophilus and larvae of monochamus alternatus. The period of validity of the pesticide in a trunk can be 2 years or longer, and the Bursaphelenchus xylophilus killing rate can be 100%.

The invention discloses a high-synchronization bursaphelenchus xylophilus resistant pinus densiflora somatic embryogenesis and plant regeneration method. The high-synchronization bursaphelenchus xylophilus resistant pinus densiflora somatic embryogenesis and plant regeneration method comprises the steps of inducement and proliferation of embryonic callus as well as maturation, germination and transformation of somatic embryos; and the maturation culture of the somatic embryos is carried out by using a liquid-solid proliferation-solid maturation method. According to the high-synchronization bursaphelenchus xylophilus resistant pinus densiflora somatic embryogenesis and plant regeneration method, with immature zygotic embryos of bursaphelenchus xylophilus resistant pinus densiflora as explants, mature somatic embryos and regenerated plants are successfully obtained by carrying out tests, such as somatic embryo maturation, germination and plant regeneration, on the embryonic callus obtained by successfully inducing the immature zygotic embryos of bursaphelenchus xylophilus resistant pinus densiflora; the mature somatic embryos and the regenerated plants are transplanted and survive; and the germination rate and plant transformation rate of the somatic embryos generated on a maturation culture medium are high and are respectively 67.2% and 46.5%; therefore, the high-synchronization bursaphelenchus xylophilus resistant pinus densiflora somatic embryogenesis and plant regeneration method provides an important scientific basis and technical support for large-scale propagation and factory production of the bursaphelenchus xylophilus resistant pinus densiflora.

The invention relates to trehalase gene Bx-tre1 and application thereof and aims to solve the problem of bursaphelenchus xylophilus spread due to the fact that stress-resistant larvae of bursaphelenchus xylophilus are capable of starting a variable percolation state to survive in a high-permeation-pressure environment. The trehalase gene Bx-tre1 is applied to pine tree disease and pest prevention and control. A nucleotide sequence of the trehalase gene Bx-tre1 is shown as Seq ID NO:1 in a sequence table. By gene silencing, the expression quantity of the trehalase gene in male bursaphelenchus xylophilus can be reduced, trehalase activity can be effectively lowered, a trehalose metabolism path is blocked, and accordingly the survival rate of the male bursaphelenchus xylophilus under high-permeation-pressure stress is remarkably decreased, and sexual propagation is further inhibited. The trehalase gene Bx-tre1 is applied to the field of bursaphelenchus xylophilus prevention and control.

The invention discloses a biological control method for bursaphelenchus?xylophilus. The biological control method comprises the following steps: firstly, taking a manually set device for trapping dastarcus helophoroide eggs to collect eggs of dastarcus helophoroides so as to obtain egg cards; placing part of the egg cards in a culture box, and performing cultivation to obtain chrysalises; and after the chrysalises emerge into bursaphelenchus xylophilus imagoes in 20-30 days, collecting the imagoes into a container to obtain imagoes. Pine disease wood piles are arranged in each bursaphelenchus xylophilus affected area, the egg cards or imagoes are put into the pine disease wood piles in March and April every year and the pine disease wood piles are enwrapped by an isolation material for isolation. Compared with the prior art, according to the biological control method disclosed by the invention, epidemic situations are controlled on the spot, and an affected area is changed into a place for naturally breeding dastarcus helophoroides, so that the labor intensity of epidemic prevention is greatly reduced, the epidemic prevention efficiency is improved, the epidemic prevention effect can be ensured for many years after the residence of the dastarcus helophoroides, and the biological control method is suitable for controlling bursaphelenchus xylophilus in mountain woods and forests.

The invention relates to a molecular detection kit for detecting trace of bursaphelenchus xylophilus in pine wood, and a method for detecting the bursaphelenchus xylophilus in pine wood by adopting the molecular detection kit. Specific amplification primers comprise a primary PCR specific primer and a nest PCR ITS specific primer, wherein the sequences of the nest PCR ITS specific primer are as follows: the upstream primer: 5'-ATGCGATTGGTGACTTCGGTT-3', and the downstream primer: 5'-CCCGCAAGGCTTCACGACGA-3'. The molecular detection kit and the method have the beneficial effects that the kit candetect trace of bursaphelenchus xylophilus from pine wood infected with diseases at the early stage, so that the judgment of the false negative result is avoided, and the detection accuracy for the trace of B. xylophilus in the pine wood is obviously improved.

The invention relates to a paecilomyces metabolite and application thereof, which belong to the technical field of microbial medicine screening. The invention is realized by the following steps that: a production strain is paecilomyces sp.1788, and the preservation code is CGMCCNo. 3422; the paecilomyces sp.1788 is fermented and cultured by czapek culture medium conventional liquid, the fermentation fluid is concentrated at low pressure, column chromatography is carried out to water phase repeatedly, and the paecilomyces metabolite of the invention is prepared after elution. The paecilomyces metabolite of the invention has the function of killing bursaphelenchuh xylophilus, the lethal rate (ED50) in 24h is 299.5ppm, and the ED95 is 796.2ppm, and can be applied to bursaphelenchuh xylophilus preparations. Compared with the traditional bursaphelenchuh xylophilus chemical, the invention is characterized by high efficiency, low toxin, water solubility, easy preparation and the like.

The invention discloses an LAMP primer group and an LAMP detection kit for detecting bursaphelenchus xylophilus by taking Bx-tlp-2 as a target gene. A plurality of pairs of LAMP detection primers are designed by taking the Bx-tlp-2 gene of the bursaphelenchus xylophilus as a target gene for LAMP detection of the bursaphelenchus xylophilus, and the LAMP primer group with high specificity and high detection efficiency is finally screened through a large number of screening tests. On the basis, the invention provides an LAMP (loop-mediated isothermal amplification) detection method and an LAMP kit for detecting bursaphelenchus xylophilus. The LAMP detection method and the detection kit provided by the invention have the advantages of simplicity and convenience in operation, low cost, high stability, strong specificity, high detection efficiency and the like, and have application prospects in pine wood nematode detection.

The invention discloses a PCR primer, a PCR detection kit and a detection method for detecting B.xylophilus. A PCR primer pair capable of specifically amplifying B.xylophilus is designed and screened by taking a conserved Bx-cpi gene in a B.xylophilus species as a target gene, and the PCR primer pair consists of two primers with nucleotide sequences shown as SEQ ID No.9 and SEQ ID No.10. According to the invention, the specific PCR primer pair is further utilized to establish a PCR detection method for B.xylophilus. The method can rapidly detect whether a sample to be detected carries the B.xylophilus. or not, and the detection method has the advantages of strong specificity, high accuracy, simple operation, short time consumption, low cost and the like. The method has application value for monitoring occurrence and spreading of the Pine Wilt Disease, overall early warning and control strategies of the Pine Wilt Disease and the like.