9 results about "Xylophilus" patented technology

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The application discloses a composition for preventing and treating Bursaphelenchus xylophilus and application thereof, and belongs to the technical field of biological pesticides. The composition is compounded by taking a crude extract of Zanthoxylum bungeanum as a main active ingredient and a crude extract of Astragalus membranaceus as a synergistic ingredient. The composition has high Bursaphelenchus xylophilus killing activity. After Bursaphelenchus xylophilus is treated by 600ug / mL of the composition solution for 24 hours, the corrected mortality of the Bursaphelenchus xylophilus reaches 99.33%. The composition can effectively prevent the occurrence of the pine wilt disease in a forest, and the control effect reaches 79.59%. In addition, the preparation process of the composition is simple, the composition is easy to use, and the composition is suitable for popularization and use.

The invention discloses a primer pair, a kit and a detection method for detecting ganoderma lucidum decay monascus xylophilus and application. The invention discloses a primer pair for detecting ganoderma lucidum spoilage monascus xylogenus. The primer pair is composed of a nucleotide as shown in SEQ ID NO.1 and a nucleotide as shown in SEQ ID NO.2. The invention further discloses a method for detecting ganoderma lucidum spoilage monascus xylogenus. The nucleotide is composed of a nucleotide shown in SEQ ID NO.3 or a nucleotide shown in SEQ ID NO.3 and a nucleotide shown in SEQ ID NO.4. The invention also discloses a kit comprising the primer pair, a method for detecting the ganoderma lucidum spoilage monascus xylogenus in a sample, a method for quantitatively detecting the content of the ganoderma lucidum spoilage monascus xylogenus in the sample and application of the primer pair. The primer and the probe with high specificity are designed, so that pathogenic bacteria causing the yellow foot disease can be accurately and quickly detected from a ganoderma lucidum sample, and the cross reaction with the DNA of the ganoderma lucidum is effectively avoided. The method is high in sensitivity, can realize early diagnosis and quantitative analysis, is matched with a kit for use, is simple and convenient to operate, provides a reliable technical means for timely early warning and accurate prevention and control of the ganoderma lucidum yellow foot disease, and is beneficial to remarkably reducing economic loss caused by the disease.

The invention belongs to the field of microbial fermentation, discloses a biocontrol compound bacteria liquid fermentation optimization process for inhibiting ficus microcarpa root rot, and aims to solve the problems of high chemical control cost, environmental pollution, drug resistance, soil micro-ecology damage by traditional pesticides and the like. The process comprises the following steps: firstly, screening bacillus subtilis and pseudomonas fluorescens from a banyan rhizosphere soil sample LB culture medium; through a plate confrontation experiment, sucrose is selected as a carbon source, yeast powder is selected as a nitrogen source, magnesium sulfate is selected as an inorganic salt, and an orthogonal experiment determines that the optimal components of the fermentation culture medium are 3% of sucrose, 1.5% of yeast powder and 1.5% of magnesium sulfate (pH 7.0). Fermentation is carried out according to specific steps, the inhibition rate of the compound bacteria liquid on harmful phellinus xylophilus is increased from 76.6% to 87.9%, the cost is low, the lasting period is 2-3 growth seasons, the influence on the diversity of soil animals is small, and banyan protection and agricultural sustainable development are promoted.

The invention discloses an optimization process of a composite charcoal fungicide for effectively inhibiting harmful phellinus xylophilus, belongs to the field of microbial fermentation optimization, and aims to solve the problem of prevention and treatment of ficus microcarpa root rot. According to the process, bacillus subtilis and pseudomonas fluorescens are subjected to seed liquid culture (LB culture medium, culture at 30 DEG C) and liquid fermentation (culture medium composed of cane sugar 30 g / L and the like, 30 DEG C and 220 rpm), then fermentation conditions (31 DEG C, pH 7.0, 24 h, 180 rpm and 10% inoculum size) are optimized, then the optimized fermentation liquid and biochar (bamboo charcoal: straw = 2: 1) are mixed according to the mass ratio of 1: 1, standing is performed for 48 h, drying is performed at 40 DEG C for 12 h, and the microbial inoculum is prepared. The bacteriostasis rate of the fungicide to harmful phellinus xylophilus reaches 85.8%, the ficus microcarpa root rot can be efficiently prevented and treated, and the application potential is large.

Provided are Lagenidium juracyae with a Bursaphelenchus xylophilus (B. xylophilus)-killing activity and a use of the Lagenidium juracyae in control of B. xylophilus. The Lagenidium juracyae has a strain number of NL03 and an accession number of CGMCC NO. 40587. In the present application, an oomycete Lagenidium sp. NL03 with a B. xylophilus-killing ability is isolated from dead soil nematodes. The biological and molecular identification and the biocontrol potential investigation are conducted for Lagenidium sp. NL03 to provide a reference for the expansion of a biological control resource pool for B. xylophilus and the eco-friendly control of pine wilt disease (PWD).

The invention relates to a composite bursaphelenchus xylophilus epidemic wood treatment bag and a bursaphelenchus xylophilus epidemic wood on-site disinfestation treatment method.The film body of the composite bursaphelenchus xylophilus epidemic wood treatment bag is of a five-layer composite structure, and the five-layer composite structure sequentially comprises an aluminum film layer, a first PE film layer, a polypropylene woven cloth film layer, a second PE film layer and a third PE film layer from outside to inside; the thickness of the aluminum film layer is 0.02 mm, the thickness of the polypropylene woven cloth film layer is 0.05 mm, the warp and weft density is 120 * 120 pieces per inch, and each piece of warp and weft is a rectangular flat filament; the thickness of each of the first PE film layer, the second PE film layer and the third PE film layer is 0.02 mm, the surface of the first PE film layer is subjected to corona treatment, the surface tension is larger than or equal to 38 mN / m, the third PE film layer is a sealed contact layer, the composite epidemic wood treatment bag and the treatment method can achieve full-scene on-site efficient pest removal of pine wood nematode disease epidemic wood, the prevention and control cost is reduced, and the prevention and control effect is improved.

The present application belongs to the field of biotechnology, and particularly relates to a chimeric dsRNA for preventing and treating pine wood nematode, a complex and application thereof. Let-526 (200 bp), EF1 (200 bp) and flp-1 (330 bp) gene fragments. The present application also provides a complex formed by delivering the chimeric dsRNA by using a chitosan nanocarrier, and a method for preventing and treating pine wood nematode by injecting the complex into a trunk. The chimeric dsRNA can simultaneously silence three key target genes, produce a synergistic lethal effect, make the mortality of pine wood nematode reach 88.8% in a pot experiment, and reduce the needle yellowing rate of a diseased black pine from 100% to 23.5%. Thus, the present application provides a new green prevention and control technology for pine wood nematode which is efficient, specific and environment-friendly.