13 results about "Xylophilus" patented technology

The invention discloses a mother strain culture medium for boletus xylophilus as well as a preparation method and application of the mother strain culture medium, and belongs to the technical field of microbial culture. The preservation number of the strain JSJ-Bx1 of the boletus sinensis mother strain is CGMCC (China General Microbiological Culture Collection Center) No.42007, and the strain JSJ-Bx1 is preserved in the China General Microbiological Culture Collection Center; the culture medium is prepared from 10-30 g of a carbon source, 2-6 g of a nitrogen source, 1-3 g of inorganic salt, 0.1-0.3 g of a growth factor suitable for the culture medium, 20 g of agar and 1 L of water, and the pH of the culture medium is 4-6. By the adoption of the culture medium, hyphae of the boletus sinensis mother strain grow fast, grow vigorously and are dense, the edges of bacterial colonies are neat, hypha degeneration phenomena such as pigment and water spitting are avoided, and the mother strain culture growth cycle is greatly shortened.

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The invention belongs to the technical field of gene engineering, and discloses a promoter and application thereof in enhancing target gene expression and promoting mannitol metabolism to synthesize bacterial cellulose. The nucleotide sequence of the promoter P00300 is as shown in SEQ ID NO: 2. The promoter is obtained by site-directed mutagenesis of a wild type promoter P06915 of gluconacetobacter xylinum and screening. The promoter provided by the invention can be used for enhancing the expression of a target gene and producing a target compound. For example, the expression level of fk, mdh1 and mdh2 three-gene combination is remarkably improved, the recombinant strain can efficiently synthesize BC by using mannitol, and the yield reaches 6.1 g / L, is about 2.87 times of that of a wild strain, and is 1.27 times of that of a bla promoter in pBla. The result lays a foundation for further understanding mannitol metabolism of gluconacetobacter xylinum and for high-value conversion and utilization of marine carbon resource mannitol and BC synthesis.

The application discloses a composition for preventing and treating Bursaphelenchus xylophilus and application thereof, and belongs to the technical field of biological pesticides. The composition is compounded by taking a crude extract of Zanthoxylum bungeanum as a main active ingredient and a crude extract of Astragalus membranaceus as a synergistic ingredient. The composition has high Bursaphelenchus xylophilus killing activity. After Bursaphelenchus xylophilus is treated by 600ug / mL of the composition solution for 24 hours, the corrected mortality of the Bursaphelenchus xylophilus reaches 99.33%. The composition can effectively prevent the occurrence of the pine wilt disease in a forest, and the control effect reaches 79.59%. In addition, the preparation process of the composition is simple, the composition is easy to use, and the composition is suitable for popularization and use.

The invention discloses a primer pair, a kit and a detection method for detecting ganoderma lucidum decay monascus xylophilus and application. The invention discloses a primer pair for detecting ganoderma lucidum spoilage monascus xylogenus. The primer pair is composed of a nucleotide as shown in SEQ ID NO.1 and a nucleotide as shown in SEQ ID NO.2. The invention further discloses a method for detecting ganoderma lucidum spoilage monascus xylogenus. The nucleotide is composed of a nucleotide shown in SEQ ID NO.3 or a nucleotide shown in SEQ ID NO.3 and a nucleotide shown in SEQ ID NO.4. The invention also discloses a kit comprising the primer pair, a method for detecting the ganoderma lucidum spoilage monascus xylogenus in a sample, a method for quantitatively detecting the content of the ganoderma lucidum spoilage monascus xylogenus in the sample and application of the primer pair. The primer and the probe with high specificity are designed, so that pathogenic bacteria causing the yellow foot disease can be accurately and quickly detected from a ganoderma lucidum sample, and the cross reaction with the DNA of the ganoderma lucidum is effectively avoided. The method is high in sensitivity, can realize early diagnosis and quantitative analysis, is matched with a kit for use, is simple and convenient to operate, provides a reliable technical means for timely early warning and accurate prevention and control of the ganoderma lucidum yellow foot disease, and is beneficial to remarkably reducing economic loss caused by the disease.

The present invention is a disposable device for collecting and separating Bursaphelenchus xylophilus, which is provided with a collection bottle, a bottle cap, funnel A, filter paper and funnel B; the collection bottle mouth of the collection bottle is internally provided with funnel A, and filter paper and funnel B are sequentially arranged in funnel A; a bottle cap convex ring is arranged inside the bottle cap, and the bottle cap and the collection bottle mouth of the collection bottle are provided with a threaded fit connection. A number of 1-3 circles of filter holes are evenly arranged on the funnel wall at the funnel mouth end of funnel A, the funnel wall and the funnel bottom are integrated, and the funnel mouth of funnel A is set as a vertical convex edge or a flat convex edge, the vertical convex edge is matched with the inner diameter of the collection bottle mouth, and the outer diameter of the flat convex edge is matched with the outer diameter of the collection bottle mouth. The filtering and separation effect of this separation device for collecting Bursaphelenchus xylophilus is good, the operation is convenient, the volume is small, and it is easy to carry. When working in the wild, it can quickly separate Bursaphelenchus xylophilus to ensure the reliability of the results, and it is suitable for medium and small forest pest control stations, etc.

The invention belongs to the field of microbial fermentation, discloses a biocontrol compound bacteria liquid fermentation optimization process for inhibiting ficus microcarpa root rot, and aims to solve the problems of high chemical control cost, environmental pollution, drug resistance, soil micro-ecology damage by traditional pesticides and the like. The process comprises the following steps: firstly, screening bacillus subtilis and pseudomonas fluorescens from a banyan rhizosphere soil sample LB culture medium; through a plate confrontation experiment, sucrose is selected as a carbon source, yeast powder is selected as a nitrogen source, magnesium sulfate is selected as an inorganic salt, and an orthogonal experiment determines that the optimal components of the fermentation culture medium are 3% of sucrose, 1.5% of yeast powder and 1.5% of magnesium sulfate (pH 7.0). Fermentation is carried out according to specific steps, the inhibition rate of the compound bacteria liquid on harmful phellinus xylophilus is increased from 76.6% to 87.9%, the cost is low, the lasting period is 2-3 growth seasons, the influence on the diversity of soil animals is small, and banyan protection and agricultural sustainable development are promoted.

The invention discloses an optimization process of a composite charcoal fungicide for effectively inhibiting harmful phellinus xylophilus, belongs to the field of microbial fermentation optimization, and aims to solve the problem of prevention and treatment of ficus microcarpa root rot. According to the process, bacillus subtilis and pseudomonas fluorescens are subjected to seed liquid culture (LB culture medium, culture at 30 DEG C) and liquid fermentation (culture medium composed of cane sugar 30 g / L and the like, 30 DEG C and 220 rpm), then fermentation conditions (31 DEG C, pH 7.0, 24 h, 180 rpm and 10% inoculum size) are optimized, then the optimized fermentation liquid and biochar (bamboo charcoal: straw = 2: 1) are mixed according to the mass ratio of 1: 1, standing is performed for 48 h, drying is performed at 40 DEG C for 12 h, and the microbial inoculum is prepared. The bacteriostasis rate of the fungicide to harmful phellinus xylophilus reaches 85.8%, the ficus microcarpa root rot can be efficiently prevented and treated, and the application potential is large.

Provided are Lagenidium juracyae with a Bursaphelenchus xylophilus (B. xylophilus)-killing activity and a use of the Lagenidium juracyae in control of B. xylophilus. The Lagenidium juracyae has a strain number of NL03 and an accession number of CGMCC NO. 40587. In the present application, an oomycete Lagenidium sp. NL03 with a B. xylophilus-killing ability is isolated from dead soil nematodes. The biological and molecular identification and the biocontrol potential investigation are conducted for Lagenidium sp. NL03 to provide a reference for the expansion of a biological control resource pool for B. xylophilus and the eco-friendly control of pine wilt disease (PWD).

The invention relates to a composite bursaphelenchus xylophilus epidemic wood treatment bag and a bursaphelenchus xylophilus epidemic wood on-site disinfestation treatment method.The film body of the composite bursaphelenchus xylophilus epidemic wood treatment bag is of a five-layer composite structure, and the five-layer composite structure sequentially comprises an aluminum film layer, a first PE film layer, a polypropylene woven cloth film layer, a second PE film layer and a third PE film layer from outside to inside; the thickness of the aluminum film layer is 0.02 mm, the thickness of the polypropylene woven cloth film layer is 0.05 mm, the warp and weft density is 120 * 120 pieces per inch, and each piece of warp and weft is a rectangular flat filament; the thickness of each of the first PE film layer, the second PE film layer and the third PE film layer is 0.02 mm, the surface of the first PE film layer is subjected to corona treatment, the surface tension is larger than or equal to 38 mN / m, the third PE film layer is a sealed contact layer, the composite epidemic wood treatment bag and the treatment method can achieve full-scene on-site efficient pest removal of pine wood nematode disease epidemic wood, the prevention and control cost is reduced, and the prevention and control effect is improved.

The utility model discloses an auxiliary device for separating bursaphelenchus xylophilus at a port, which is characterized in that a supporting filter screen is arranged in a separating cylinder, and a filter screen is laid on the supporting filter screen; the pressing pipe is inserted into the separation cylinder, and a permeable filter screen is arranged at the lower end of the pressing pipe; the inlet of the valve body is connected with the lower end of the separation cylinder; the solution bottle can be arranged below the valve body, and the open end of the solution bottle can be aligned with the outlet of the valve body. According to the utility model, the supporting filter screen is horizontally fixed in the separation cylinder, so that the supporting filter screen can support the filter screen; the filter screen can intercept and filter the wood blocks, so that only pine nematodes can pass through the filter screen, and large-particle-size impurities in the wood blocks are prevented from entering the solution bottle; the downward pressing pipe drives the permeable filter screen to move downwards so as to press the wood blocks into water, and water in the separation barrel cannot overflow in the downward moving process of the downward pressing pipe; in addition, the downward pressing pipe can be arranged in place firstly, then water is added into the separation barrel, and in this way, it is guaranteed that the wood blocks are completely immersed in water, and water can be conveniently added.

The present application belongs to the field of biotechnology, and particularly relates to a chimeric dsRNA for preventing and treating pine wood nematode, a complex and application thereof. Let-526 (200 bp), EF1 (200 bp) and flp-1 (330 bp) gene fragments. The present application also provides a complex formed by delivering the chimeric dsRNA by using a chitosan nanocarrier, and a method for preventing and treating pine wood nematode by injecting the complex into a trunk. The chimeric dsRNA can simultaneously silence three key target genes, produce a synergistic lethal effect, make the mortality of pine wood nematode reach 88.8% in a pot experiment, and reduce the needle yellowing rate of a diseased black pine from 100% to 23.5%. Thus, the present application provides a new green prevention and control technology for pine wood nematode which is efficient, specific and environment-friendly.