2 results about "Xylophilus" patented technology

Provided are Lagenidium juracyae with a Bursaphelenchus xylophilus (B. xylophilus)-killing activity and a use of the Lagenidium juracyae in control of B. xylophilus. The Lagenidium juracyae has a strain number of NL03 and an accession number of CGMCC NO. 40587. In the present application, an oomycete Lagenidium sp. NL03 with a B. xylophilus-killing ability is isolated from dead soil nematodes. The biological and molecular identification and the biocontrol potential investigation are conducted for Lagenidium sp. NL03 to provide a reference for the expansion of a biological control resource pool for B. xylophilus and the eco-friendly control of pine wilt disease (PWD).

The present application belongs to the field of biotechnology, and particularly relates to a chimeric dsRNA for preventing and treating pine wood nematode, a complex and application thereof. Let-526 (200 bp), EF1 (200 bp) and flp-1 (330 bp) gene fragments. The present application also provides a complex formed by delivering the chimeric dsRNA by using a chitosan nanocarrier, and a method for preventing and treating pine wood nematode by injecting the complex into a trunk. The chimeric dsRNA can simultaneously silence three key target genes, produce a synergistic lethal effect, make the mortality of pine wood nematode reach 88.8% in a pot experiment, and reduce the needle yellowing rate of a diseased black pine from 100% to 23.5%. Thus, the present application provides a new green prevention and control technology for pine wood nematode which is efficient, specific and environment-friendly.