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Compound with pine wood nematode killing activity with poison and use thereof

A technology of pine wood nematodes and compounds, applied in the fields of application, organic chemistry, and biocides, can solve the problems of strong drug resistance, no research reports on pine wood nematodes and monochamus alternatus agents or methods, unsatisfactory effects, etc. question

Inactive Publication Date: 2006-10-25
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is only suitable for protecting precious trees, and the effect is not ideal, and it is highly toxic to humans and animals
[0005] At present, the method of controlling pine wood nematode disease is mainly based on spraying insecticides to control Monochamus alternatus, but due to the long adult period and strong drug resistance of Monochamus alternatus, the control effect is not ideal
So far, no effective and low-toxic special pesticides for killing pine wood nematodes that can be practically applied have been studied, and there are no research reports on agents or methods for both treating pine wood nematodes and Monochamus alternatus

Method used

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  • Compound with pine wood nematode killing activity with poison and use thereof
  • Compound with pine wood nematode killing activity with poison and use thereof
  • Compound with pine wood nematode killing activity with poison and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the separation and preparation of compound 1-6

[0023] a. Seed culture of the fungus Gliocladium roseum Gr87:

[0024] PDA medium: 200g of potatoes, 20g of glucose, 1.0g of yeast extract, 6.0g of glycerol, 17g of agar, 1000mL of water, make a test tube slant, pick the strains into the slant, and culture at 28°C for 5 days;

[0025] b. Solid fermentation culture of the fungus Glioma pink:

[0026] Wheat culture medium (the preparation process is: soak the wheat in tap water for 48 hours, then wash it, drain the water, put it into a 500cm Erlenmeyer flask, wrap the mouth of the bottle with cotton cloth and sterilize twice at 15psi, 40min each time).

[0027] Pick the strain cultured on the slant into the solid fermentation medium, and culture it statically at room temperature 25°C for 30 days;

[0028] c. freeze-drying the mycelia of Glioma pinka that has been cultivated above, and extracting it by cold immersion in an optional organic solvent (such as me...

Embodiment 2

[0033] The liquid immersion method is used to detect the anti-nematode activity of the monomer and the mixture of the compound of the present invention.

[0034] 1. Test drug:

[0035] Dissolve the test sample in a small amount of organic reagent DMSO, and then add a certain concentration of Tween aqueous solution to prepare sample test solutions with the concentrations shown in Table 4. In this solution, the final concentration of DMSO is <3%, and the final concentration of Tween is <5‰. In addition, the same concentration of DMSO was dissolved in an aqueous solution containing 0.5% (V / V) Tween-20 as a control.

[0036] 2. Cultivation and preparation of pine xylophilus

[0037] The cultivation method of pine wood nematode B.xylophilus: insert Botrytis cinerea into a PDA plate, culture at 25°C until the plate is full, insert a medium with B.xylophilus, and culture at 25°C Until the mycelium disappears, the nematodes cover the plate.

[0038] When in use, pick out the mediu...

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Abstract

The invention relates to a class of compounds with the activity of poisoning and killing pine xylophilus and applications thereof, belonging to the technical field of biological pesticides. Such active compounds are obtained from production strains by conventional solid fermentation and extraction and separation methods. Its production strain is Gliocladium roseum (Gliocladium roseum) Gr87, which has been preserved in the China Committee for the Preservation of Microorganisms on October 9, 2002, with a preservation number of CGMCC No.0807; this type of active compound consists of 6 compounds The composition can effectively poison and kill pine xylophilus, and can be used alone or in combination to prepare nematicidal biopesticides.

Description

Technical field: [0001] The invention relates to a class of compounds with the activity of poisoning and killing pine xylophilus and applications thereof, belonging to the technical field of biological pesticides. Background technique: [0002] Pine wood nematode disease is a devastating disease caused by the rapid death of trees caused by pine wood nematode (Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle) parasitizing in the body of Pinus sp. The important quarantine object is listed as the first of forest diseases and insect pests, and is called "smokeless forest fire". Since the disease was discovered in 1905 and reported in 1934, it has been widely distributed in Japan, the United States, Korea, Canada, Mexico, Greece, Portugal and China. Japan's annual cost for the prevention and control of this disease accounts for 93.6% of the cost of forest pest control, accounting for 20% of the total forestry investment, but the annual loss of wood due to this disease still ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D513/22A01N63/02
Inventor 董锦艳张克勤
Owner YUNNAN UNIV
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