The invention discloses a brassinolide neem microcapsule which comprises the following compositions in percentage by weight: 0.1-20 neem, 0.001-20 brassinolide, 10-40 capsule wall materials, 5-30 solvent, 1-20 emulsifier, 5-30 synergist and the balance of water. The invention belongs to the field of biological pesticides. Concretely, in the invention, the brassinolide and the neem are mixed, and a right amount of assistant is added to prepare the brassinolide neem microcapsule, therefore, the brassinolide neem microcapsule is beneficial to natural ecological environment, has wide control range to agricultural and forest pests, is difficult to generate drug resistance of the pests, has obvious action of promoting crop growth and has durable efficacy, thereby achieving the effects of low toxicity, safety to humans, livestock and poultry, high efficacy and long durability without pollution.