270 results about "Nematode" patented technology

The invention discloses a pest control management system and method for forest tree breeding, and relates to the technical field of forest tree breeding and seedling raising, and the method comprises the following steps: collecting environment data and soil rhizosphere data by collecting multi-mode images of leaves and trunks of a pinus massoniana young forest and videos that pinus massoniana caterpillars gnaw leaves and cluster diffusion; constructing a disease and insect pest recognition model by using a convolutional neural network, performing definite diagnosis on pine wood nematode disease, pine caterpillars and monochamus alternatus hope, and quantifying the disease and insect pest occurrence area and population density of each sub-region; calculating a pine wood nematode disease control demand index, comparing the pine wood nematode disease control demand index with a threshold value, and triggering a corresponding control strategy; the diffusion coefficient of the pine caterpillars is calculated and compared with a risk threshold value, and a prevention and control scheme is formulated; calculating the propagation coefficient of the monochamus alternatus hope, judging the propagation risk and giving a prevention and control strategy; according to the invention, intelligent pest identification, risk quantification and control strategy closed-loop control are realized, treatment and scientific prevention and control are facilitated, and the pest control efficiency of the pinus massoniana young forest and the forest stand health level are improved.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus agave, fermentation liquor, a biological agent and application. The invention discloses a strain of bacillus tequilensis, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus tequilensis is CGMCC NO.34830. The strain of bacillus tequilensis has the advantages that the preservation number is CGMCC NO.34830; the invention also discloses a fermentation broth and a biological agent prepared according to the bacillus agave, and the bacillus agave, the fermentation broth and the biological agent provided by the invention can effectively reduce the harm of root knot nematode disease to plants and promote seed germination or crop growth, and have the advantages of high efficiency, safety, environmental protection and difficult generation of resistance.

The invention discloses a microbial agent and application thereof in agricultural production. The microbial agent is prepared by compounding seed solutions of two or more of seed solutions of Trichoderma harzianum, Trichoderma brevicompactum Tb12, Gliocladium roseum NF-06 and Trichoderma asperellum, and the seed solutions of the two or more of the Trichoderma harzianum, the Trichoderma brevicompactum Tb12, the Gliocladium roseum NF-06, the Trichoderma asperellum, the Trichoderma harzianum, the Trichoderma brevicompactum, the Gliocladium roseum NF-06 and the Trichoderma asperellum. The selected strain combination can effectively prevent and treat fungal diseases and nematode diseases and can promote plant growth. The trichoderma harzianum and the trichoderma brevicompactum Tb12 are used in a combined mode, the control effect on fusarium is obvious, the gliocladium roseum NF-06 and the trichoderma asperellum are used in a combined mode, the nematode killing effect is obvious, and the growth of rice is promoted through the trichoderma harzianum and the trichoderma asperellum.

The invention relates to the technical field of pest prevention and control, in particular to a method for treating pine wood nematode infected wood through poria cocos. The method comprises the following steps: cutting down current-year epidemic wood, and treating tree heads, tree trunks and boughs to complete peeling and tendon retaining treatment; uniformly cutting the trunk and the boughs into standard cut-logs; the method comprises the following steps: selecting fresh poria cocos sclerotium skin tissues as strains, and cutting according to an area greater than 50% of a cross section; pit pits are dug in the sunny slope; laying small branches on the bottom layer, placing the cut-logs in the pits, fixing the cut-logs with non-woven fabrics after inoculation, covering the upper layer with thin branches and leaves, and covering with soil; the two-stage biological process of mycelium planting, propagation expanding and pine wood nematode and medium insect killing is sequentially completed through a soil heat and moisture preservation environment; the propagation path of pine wood nematodes is blocked through the natural isolation effect of soil, deadwood obtained after poria cocos is subjected to biological verification and then smashed on site to return to forests or be made into fuel particles, and harmless and resource recycling of epidemic wood is achieved. According to the method, the problems of pine wood nematode propagation and epidemic wood treatment are synchronously solved.

Compounds of the formula (I) wherein Q is as defined in claim 1. Furthermore, the present invention relates to agrochemical compositions which comprise compounds of formula (I), to preparation of these compositions, and to the use of the compounds or compositions in agriculture or horticulture for combating, preventing or controlling animal pests, including arthropods and in particular insects, molluscs, nematodes or representatives of the order Acarina.

Disclosed in the present invention are a triazine derivative, a preparation method therefor and the use thereof, belonging to the technical field of drug synthesis. Specifically, disclosed in the present invention are a triazine derivative represented by general formula (I), a pharmaceutical composition containing the triazine derivative, and the use thereof in the preparation of an anthelmintic drug for humans or animals. The general structural formula (I) of the triazine derivative is shown as follows. The triazine derivative of the present invention has a significant effect in resisting zoonotic helminths such as nematodes and heartworms, and has a broad application prospect.

The invention provides a pine pollen fermentation product with anti-wrinkle and anti-aging functions as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The pine pollen extract is prepared by fermenting lactobacillus plantarum, the total polysaccharide content of the obtained pine pollen fermentation product is maximally increased by 77.3%, the FRAP total antioxidant capacity is maximally increased by 2.85 times compared with that of the unfermented pine pollen fermentation product, the anti-wrinkle effect of human skin cells can be effectively improved, and the pine pollen extract has a relatively strong proliferation effect on an ultraviolet damage model of human skin fibroblasts; the pine pollen extract has the advantages that the pine pollen extract is fermented, the elastin content is remarkably increased, the life of nematodes is effectively prolonged, lipofuscin deposition is reduced, and compared with unfermented pine pollen extract, the anti-aging capacity of the pine pollen can be effectively improved; the preparation method provided by the invention is low in price and simple in process, and the active ingredients and the efficacy of the traditional Chinese medicine composition are improved in a simplest and effective manner.

The application discloses a nematicidal composition and application thereof, wherein the composition contains active ingredient A and active ingredient B, the weight ratio of the active ingredient A to the active ingredient B is 1:50-50:1, the active ingredient A is a compound shown as a formula I, and the active ingredient B is a nematicidal thiazole. The nematicidal composition is used for preventing and treating plant parasitic nematodes, has good nematicidal activity, and has little toxicity to human and animals.

The invention relates to a multi-channel controllable pine wood nematode collection culture dish and a culture and collection method, and belongs to the technical field of pine wood nematode culture.The multi-channel controllable pine wood nematode collection culture dish comprises a culture dish body, the culture dish body comprises a culture dish cover and a culture dish body, the culture dish cover is provided with three sampling holes, sampling pipes are installed at the sampling holes, and the three sampling holes are communicated with the culture dish body. The three sampling tubes are annularly arranged along the center of the culture dish cover. According to the method, the natural enrichment phenomenon is utilized, the sampling pipe and the sample leaking hopper are matched, water drops containing the nematodes are collected through gravity, compared with a traditional filtering centrifugal collection method, the traditional time-consuming filtering step is omitted, the nematodes can be used after being extracted, the operation process is remarkably simplified, the nematode collection time is greatly shortened, the working efficiency is improved, and the method is suitable for popularization and application. And the three sampling pipes synchronously collect and continuously collect after the sampling pipes are replaced, so that the resource utilization rate is increased.

The invention relates to the technical field of chemical toxicology, in particular to an evaluation method for comprehensively analyzing the immune induction effect of a plasticizer by using caenorhabditis elegans, according to the evaluation method, a mother worm breeding mode is changed to replace a lysis solution to synchronize nematodes, experimental interference is reduced, the result accuracy is improved, monitoring indexes are newly increased, intestinal injuries and in-vivo bacterial indexes are increased, and the evaluation method is suitable for comprehensive analysis of the immune induction effect of the plasticizer. The evaluation is more accurate and reliable by combining oxidative stress indexes and performing two-dimensional comprehensive evaluation from an intestinal immune defense line and oxidative stress, and by drawing a multi-index monitoring shape graph, adopting a comprehensive biomarker response index analysis method and associating immune-related representative indexes, the evaluation accuracy is improved. According to the method, the change of each index and the immunity intensity on the caenorhabditis elegans under different exposure conditions are intuitively presented, the immune defense effect of the caenorhabditis elegans under the stress of the plasticizer can be quickly and effectively evaluated without transcriptome and metabonomics, and efficient evaluation is realized.

The invention discloses an application of nitrone compounds in preparation of related products for improving athletic ability. The nitrone compound disclosed by the invention has a structure shown in a general formula (I), and experimental results show that the nitrone compound disclosed by the invention can be used for increasing the exercise capability of nematodes of different age groups, including the body swing frequency and the pharyngeal pumping rate.

This invention discloses a pesticide composition for controlling guava nematodes and its preparation method, belonging to the field of pesticide technology. By weight, the raw materials of the pesticide composition include the following components: 8-12 parts of neem seed extract, 8-12 parts of pyrethrum extract, 5-8 parts of tongkat ali extract, 5-8 parts of croton fruit peel extract, 5-8 parts of guava leaf extract, 20-25 parts of compound microbial agent, 3-5 parts of sodium lignosulfonate, 5-8 parts of xanthan gum, 4-6 parts of trehalose, and 80-100 parts of water. The above components work synergistically to achieve excellent control of guava nematodes.

To provide a process for forming arylcyclopropylcarboxylic acids useful for forming molecules having insecticidal applications against pests in the phyla Arthropoda, Mollusca, and Nematoda.SOLUTION: There is provided an enantiomerically enriched preparation of the compound (R2, R4) - 3 - (3, 5-bis (trifluoromethyl) phenyl) - 2, 2-dichlorocyclopropane-1-carboxylic acid in a heterogeneous S3a, wherein CF3 and R3 are; R2, and R5 are H; and R7 and R8 are Cl; wherein the enantiomeric excess of the (R, R) - enantiomer of the is greater than 80%, greater than 90% or greater than 95%. 1R 3R S3a. Also provided are pesticidal formulations comprising said enantiomerically enriched preparations.SELECTED DRAWING: None

The application discloses a kind of nematode body bending behavior identification and counting method and related equipment, the method comprises: obtaining nematode video, the original gray image is obtained by frame processing to nematode video, and binary image is obtained according to the original gray image of nematode;According to the original gray image of nematode, the numerical coordinate regression algorithm based on convolutional neural network obtains head coordinate and tail coordinate;According to the binary image of the nematode, the feature point extraction algorithm based on curvature calculates the peak point on the center line of nematode;The maximum distance of the line between the pharynx and tail of nematode is calculated, and the number of nematode body bending is calculated according to the change of maximum distance of each frame.The numerical coordinate regression algorithm based on convolutional neural network is used for the coordinate identification of nematode head and tail, the accuracy is improved, and the number of nematode body bending is calculated more simply and quickly by extracting feature points.

The invention provides bacillus subtilis for killing nematodes and promoting plant growth and application thereof. The bacillus subtilis strain provided by the invention is a bacillus subtilis Shannan.BS84-62 strain, and is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M 2024387. The bacillus subtilis strain provided by the invention is a bacillus subtilis strain which is preserved in the China Center for Type Culture Collection (CCTCC). The bacillus subtilis Shannan.BS84-62 strain provided by the invention has the characteristic of killing nematodes, has the functions of producing indoleacetic acid, fixing nitrogen and dissolving phosphorus and potassium, and can promote plant growth.

The invention discloses application of 3, 5, 6-trihydroxy-stilbene glucoside in preparation of an anti-aging product and an aging inhibitor. Researches find that the 3, 5, 6-trihydroxy-stilbene glucoside has the effects of delaying senescence of nematodes and prolonging the life of the nematodes, can reduce accumulation of lipofuscin of the nematodes, improve exercise ability and enhance stress resistance, so that senescence of the nematodes is delayed, the life of the nematodes is prolonged, and the 3, 5, 6-trihydroxy-stilbene glucoside can be used for preparing the medicine for resisting senescence and / or prolonging the life.

The invention discloses a strain of parateratomycosis aveosphaeroides and application thereof, and belongs to the technical field of microorganism and biological prevention and control. In order to screen new species of fungi and provide alternative strains for application of plant beneficial bacteria in agriculture, a fungus SGSF1293 is separated, and the fungus SGSF1293 is identified to be a new species of the cystis clavatum and is named as the parateratosis aveoensis, and the new species of the cystis clavatum is named as the parateratosis aveoensis. According to the present invention, the activity research results show that the strain SGSF1293 has rice root-knot nematode killing activity, plant pathogen resistance (Brassica oleracea black rot xanthomonas, Pseudomonas syringae, Ralstonia solanacearum and Fusarium oxysporum) resistance, oxidation resistance and phosphorus solubilizing activity; besides, the secondary metabolite of the strain is separated and identified, two active compounds, namely, wintergreen ambrotic acid and brefeldin A, are separated, and the wintergreen ambrotic acid has the activity of killing rice root-knot nematode. The discovery of the strain SGSF1293 and the active metabolite thereof has certain application value in the agricultural fields of plant disease control and the like.

The invention relates to the field of pesticides, and discloses an isoxazoline compound containing a pyridine bisamide structure, application of the isoxazoline compound and a pesticide, and the isoxazoline compound has a structure as shown in a formula (J). The compound provided by the invention can realize good effects of killing insects, mites, nematodes and spiroworms at low concentration.

Compounds of the formula (I) wherein the substituents are as defined in claim 1. Furthermore, the present invention relates to agrochemical compositions which comprise compounds of formula (I), to preparation of these compositions, and to the use of the compounds or compositions in agriculture or horticulture for combating, preventing or controlling animal pests, including arthropods and in particular insects, nematodes, molluscs or representatives of the order Acarina.

The invention relates to the technical field of biological detection, and discloses a metabonomics-based caenorhabditis elegans compound pollutant exposure evaluation method which comprises the following specific steps: S1, constructing a compound pollutant exposure system; s2, carrying out nematode culture and exposure treatment; s3, extracting in-vivo metabolites of the nematodes; s4, carrying out metabonomics analysis; s5, carrying out bioinformatics analysis; and S6, verifying the combined toxicity effect. By establishing the metabonomics-based caenorhabditis elegans compound pollutant exposure evaluation method, the limitation of a traditional single pollutant evaluation system is broken through, and systematic and high-sensitivity detection of multi-pollutant combined toxicity is realized; in addition, by constructing a standardized composite exposure system and an efficient metabolite extraction process and combining multi-dimensional mass spectrum data and bioinformatics modeling, a synergistic, antagonistic or additive effect mechanism among pollutants is deeply analyzed from the molecular level.

The invention discloses an LAMP primer designed by taking a BXPC1 gene of bursaphelenchus xylophilus as a target gene, CRISPR RNA and application of the LAMP primer. The invention designs an LAMP (loop-mediated isothermal amplification) primer and CRISPR (clustered regularly interspaced short palindromic repeats) RNA (ribonucleic acid) by aiming at a BXPC1 gene of the pine wood nematodes as a target gene and establishes a detection kit and a detection method for detecting the pine wood nematodes based on an LAMP-CRISPR / Cas12a system. Specificity and sensitivity tests show that the CRISPR RNA as shown in SEQ ID No.8 has the best detection effect, pine wood nematode and bursaphelenchus mucronatus can be specifically distinguished, and the sensitivity reaches 0.1 pg / L; the method for detecting the pine wood nematodes based on the LAMP-CRISPR / Cas12a system has the advantages of being good in stability, high in practicability, high in amplification efficiency and the like, and a sensitive, specific, rapid and visual detection means is provided for rapidly screening the pine wood nematodes on site.

The application provides a method for preventing and treating monochamus alternatus, which comprises putting male monochamus alternatus carrying Wolbachia into a target prevention and treatment area of monochamus alternatus not carrying Wolbachia. The method has low investment cost, is green, safe, environment-friendly, has no pesticide residue and high risk, conforms to the green prevention and control principle and needs of diseases and pests in the new era, is less affected by external temperature, humidity and other climate environments, is suitable for wide plots, is a new prevention and treatment method, is early in the prevention and treatment period, prevents and treats from the egg stage, reduces the double hazards of the larval stage dry woodworm and the pupal stage adult stage spreading pine wood nematode disease from the root, has good prevention and treatment effect, and can significantly reduce the egg hatching rate of monochamus alternatus by more than 90%.

The invention belongs to the technical field of cosmetic detection, and relates to a method for evaluating the skin barrier repair effect of cosmetics and application of the method, and the method comprises the following steps: carrying out grouping culture on nematodes after the same period: a culture of a sample group contains a to-be-detected sample; the culture of the control group does not contain the sample to be detected, and other culture conditions are consistent with those of the sample group; the cultured nematodes are collected, nematode precipitates are centrifugally taken and dyed, the dyed nematodes are subjected to fluorescence intensity detection, and if the fluorescence intensity of the nematodes in the sample group is remarkably different from that of the nematodes in the control group, the sample to be detected has the skin barrier repairing effect. The method for evaluating the repairing effect of the cosmetic is provided on the basis of caenorhabditis elegans, the repairing degree of the product to the caenorhabditis elegans is judged by measuring the fluorescence intensity of the caenorhabditis elegans, and then the repairing effect of the product is reflected. The experimental period is short, the experimental consumable cost is low, and ethical conflicts do not exist.

The application provides a kind of high biocompatibility biological silver nanoparticles, its preparation method, compound microcapsule and application, the biological silver nanoparticles are based on the fermentation broth or fermentation filtrate of biocontrol bacillus B268, the metabolite of B268 is combined on the surface of particle, the antagonistic activity to plant pathogenic bacteria is enhanced, and show high biocompatibility to biocontrol bacteria, biocontrol fungi, plant, model nematode, can be directly embedded with bacteria liquid, the microcapsule prepared fuses the compatible interaction relationship between bacteria, bacterial metabolite, silver nanoparticles, shows synergistic antibacterial effect, and the bacteriostatic activity is higher than that of microcapsule embedded by bacteria liquid or silver nanoparticles alone, and under low temperature preservation condition, microcapsule can keep the stability of bacteria, silver nanoparticles and its synergistic antibacterial activity, provides preparation raw material and preparation method for developing nano biological pesticide.

This invention discloses a method for establishing a spectral characteristic database of trees affected by wood nematode, involving the interdisciplinary fields of forestry disease monitoring, spectral analysis, and database technology. By capturing subtle spectral changes in pine needles in the red and near-infrared bands, this invention can identify signs of infection before obvious symptoms appear on the tree's surface. Through a multi-step, multi-index comprehensive analysis method, it effectively eliminates interference from other ground features such as forest land and bare soil. The established dedicated spectral model and indices are highly targeted, achieving higher classification and identification accuracy than using a single general index, significantly reducing the false positive rate. The use of UAV hyperspectral remote sensing technology overcomes the shortcomings of manual inspection, such as limited coverage, numerous blind spots, and poor timeliness, enabling rapid and comprehensive monitoring of large-scale pine forest resources. It can not only determine whether pine trees are infected but also quantitatively assess the severity of the disease through chlorophyll content estimation models and spectral characteristic differences.

The invention provides an arthrobotrys oligospora genetic engineering strain with nematode attracting activity, and belongs to the technical field of microbial genetic engineering, the genetic engineering strain takes arthrobotrys oligospora as a starting strain, and the genetic engineering strain is a strain obtained by knocking out an AoETF beta gene in an arthrobotrys oligospora genome. The arthrobotrys oligospora genetic engineering strain has nematode attraction activity, and can attract nematodes in soil to gather together so as to achieve the effect of efficiently capturing and killing the nematodes, thereby providing a basis for biological control of the nematodes. The invention further provides a construction method and application of the arthrobotrys oligospora genetic engineering strain with nematode attracting activity.

Compositions and methods for modulating a microbiome are disclosed. In accordance with the instant invention, methods of modifying and / or modulating a microbiome (e.g., the composition of a microbiome) are provided. The methods of the instant invention comprise contacting the microbiome (e.g., the assemblage of organisms composing the microbiome) with a compound excreted by an organism (e.g., nematode), particularly an organism that naturally lives in or is part of the microbiome.

The application discloses Bifidobacterium Pseudocatenulatum and application thereof, and the Bifidobacterium Pseudocatenulatum YSBP01CCTCC NO: M 20232067. Experiments prove that the Bifidobacterium Pseudocatenulatum can enhance the antioxidant capacity of Caenorhabditis elegans, prolong the life of the nematode, improve the activity and the reproductive capacity of the nematode, reduce fat accumulation, improve the mitochondrial membrane potential, and delay the aging of the body. The Bifidobacterium Pseudocatenulatum can also significantly inhibit the weight increase of a high-fat-diet mouse, reduce the TG level in serum of the mouse, significantly reduce the fasting blood glucose concentration, and effectively prevent and relieve obesity and type 2 diabetes.