1961 results about "Nematode" patented technology

Methods are provided for the genetic control of pathogen infestation in host organisms such as plants, vertebrate animals and fungi. Such methods utilize the host as a delivery system for the delivery of genetic agents, preferably in the form of RNA molecules, to a pathogen, which agents cause directly or indirectly an impairment in the ability of the pathogen to maintain itself, grow or otherwise infest a host plant, vertebrate animal or fungus. Also provided are DNA constructs and novel nematode nucleotide sequences for use in same, that facilitate pathogen resistance when expressed in a genetically-modified host. Such constructs direct the expression of RNA molecules substantially homologous and/or complementary to an RNA molecule encoded by a nucleotide sequence within the genome of a pathogen and/or of the cells of a host to effect down-regulation of the nucleotide sequence. Particular hosts contemplated are plants, such as pineapple plants, and particular pathogens are nematodes.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

The invention relates to identifying and evaluating target coding sequences for control of plant parasitic nematodes by inhibiting one or more biological functions, and their use. The invention provides methods and compositions for identification of such sequences and for the control of a plant-parasitic nematode population. By feeding one or more recombinant double stranded RNA molecules provided by the invention to the nematode, a reduction in disease may be obtained through suppression of nematode gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and the plant cells and plants obtained thereby.

The invention provides compositions comprising polynucleotide molecules encoding certain pesticidal polypeptides which exhibit plant parasitic nematode and/or insect control properties, and are particularly directed to controlling plant parasitic pest species of nematodes and insects known to infest crop plant species. Methods for controlling pests are disclosed in which the toxic proteins are provided in the diet of the targeted plant pests. The invention also provides compositions such as nucleic acids, proteins, and plant and bacterial cells, plants, and seeds containing the nucleic acid and protein compositions, as well as methods and kits for identifying, detecting, and isolating the compositions of the present invention. The invention further provides a method of producing crops from recombinant seeds which contain the polynucleotide molecules encoding the pesticidal polypeptides of the present invention.

Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for nematicidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nematicidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 8, 9, 13, 14, 47, 48, or 49, the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 6, 7, 10, 11, 12, 15, 45, or 46, as well as variants and fragments thereof.

The invention relates to a planting method of Chinese trichosanthes, belonging to the technical field of plantation and comprising the steps of: variety breeding, seedling reproduction, transplanting, field management, disease and pest control, harvesting and processing, wherein the variety breeding step includes the sub-steps of the tests in different production places, amplification and verification tests, resistance test, field pest investigation and trichosanthes nematode control; the seedling reproduction step includes the sub-steps of seed reproduction and root reproduction; the transplanting step includes the sub-steps of land selection and preparation, ridging, reproduction and planting, planting method implementation and field management; the land selection and preparation step includes the sub-steps of detection on chemical characteristic of soil, basic fertilizer application and disinfection; the reproduction and planting step includes the sub-steps of seed bud selection, rood cutting and breeding, planting and shed putting up; the planting method implementation step includes the sub-steps of planning density control and staminiferous plant matching; the filed management step includes the sub-steps of intertillagement and weeding, top application, shed putting up, pruning and cold prevention, in particular to intertillagement and weeding, root airing, intercrop, earthing, vine supporting and racking, top application, pruning, flower and fruit protection, chemical control and foliage application; and the disease and pest control step includes the sub-steps of control of diseases and pest and control method implementation.

Functionalized polymers are mixed with pesticides to form semi-stable complexes with desirable field properties: reduced leaching in soil, improved leaf retention (rainfastness), selective unloading to roots and convenient packaging and application. Pesticides that may be so complexed include herbicides, insecticides (including compounds controlling non-insect arthropods and nematodes), bacteriocides, rodenticides, and fungicides. Polymers with which they may be complexed include derivatives of carbohydrates, amides, imines, alkanes, vinyls, styrenes or glycols. The polymers may be functionalized with chemical groups that exhibit ionic (amines, carboxyls), hydrophobic, complexing (e.g. metal chelating) and ligand binding interactions. The variously functionalized polymers may be mixed, grafted, or fused to obtain optimal properties. The polymer/pesticide formulations may be applied as granules, as suspensions or solutions in sprays, as foams, or as coats for seeds and fertilizers. The formulations may be applied to foliage, soil, irrigation water, construction materials (plastics, wood), seeding materials, grains, and buildings.

The invention belongs to the field of plant protection, and in particular relates to an agricultural composition taking oligosaccharide and sulphur as synergistic components and an application of the agricultural composition in prevention and treatment of agricultural pests. The mass part ratio of oligosaccharide to sulphur is 1:(0.1-200) and the mass part ratio of oligosaccharide to sulphur to other pesticide components is 1:(0.1-200):(0.1-100). In the composition, oligosaccharide is pectin oligosaccharide, sodium alginate oligosaccharide or chitosan oligosaccharide. The synergistic composition containing oligosaccharide and sulphur can be processed to particles, suspending agents and the like with other insecticidal and mite-killing components or sterilizing components to realize the synergistic effect of the agricultural composition, and the agricultural composition is used for preventing and treating nematode, red spiders, Phyllocoptruta oleivora Ashmead or powdery mildew of crops.

The present invention concerns double stranded RNA compositions and transgenic plants capable of inhibiting expression of essential genes in parasitic nematodes, and methods associated therewith. Specifically, the invention relates to the use of RNA interference to inhibit expression of a target essential parasitic nematode gene, which is a parasitic nematode pas-5 gene, and relates to the generation of plants that have increased resistance to parasitic nematodes.

The invention provides a pesticide fertilizer and application thereof. The pesticide fertilizer is prepared from the following components in percentage by weight: 0.1-1 percent of abamectin, 1-10 percent of imidacloprid and/or acetamiprid and/or thiamethoxam, 15-40 percent of great amount of element fertilizer, 1-5 percent of trace element fertilizer and 44-72.4 percent of carrier. By compounding the abamectin, the imidacloprid and/or the acetamiprid and/or the thiamethoxam, a great amount of element fertilizer and the trace element fertilizer, the pesticide fertilizer effectively improves the pesticide effects of the abamectin, the imidacloprid and/or the acetamiprid and/or the thiamethoxam on nematodes and soil pests and takes the effect of promoting the growth of crops. Because of the excellent performance and the pesticide effects, the pesticide fertilizer can be applied to the prevention of the nematodes and the soil pests. The invention also provides preparation methods of a grain-type pesticide fertilizer and a powder-type pesticide fertilizer, which have simple process and easily controlled conditions and are suitable for industrial production.

An isolated DNA fragment that promotes root knot or cyst nematode indcuible transcription of a coding sequence downstream of and operably linked to the fragment in at least an Arabidopsis thaliana plant and a chimeric DNA sequence including the DNA fragment. Also, methods for the use of the DNA fragment.

The instant invention relates to compositions and methods for the control of nematodes and soil borne diseases using compositions comprising high terpene containing oils and one or more surfactants. The invention also relates to methods for increasing the wetted soil volume available for water utilization by plant roots using the disclosed compositions.

The present invention discloses a non-social pollution drug fertilizer for preventing wireworm and underground pest and the relative technics process; the drug fertilizer consists of the following components according to the weight ratio: 1-10 percent of bitter bean, 1-10 percent of cattle heart Preserving Simplicity, 1-10 percent of azedarach fruit, 1-3 percent of carapace or chitosan, 0.5-2 percent of salicylic acid or natrium salicylicum, 1-3 percent of Chlopyrifos or phoxim, 1-5 percent of diffusing agent, 1-5 percent of surface active agent, 1-10 percent of adhesive agent, 5-15 percent of disintegrant, 5-20 percent of micro fertilizer, 10-50 percent of corrupted acid; wherein, the weight ratio of all components is 100 percent. The drug fertilizer in the present invention integrates nutrient, improving soil and preventing pest together, which has much better immunity, prevention and curing effect to the wireworm and underground pest. The present invention can realize the purpose for ''detoxification to the soil and epidemic prevention to the crops'', decrease the use quantity for fertilizer and venomous pesticide and enhance the biological stress for the soil and crops. The present invention does not leave in the crop and pollute the environment. The present invention can accelerate the growth for the crops, decrease the pests in the crops, increase the output and improve the quality.

The invention discloses a biological pesticidal organic fertilizer and a preparation method thereof. The biological pesticidal organic fertilizer is prepared from the following raw materials in parts by weight: 40-50 parts of pig manure, 20-30 parts of tea seed pulp, 10-15 parts of coffee grounds, 8-12 parts of paulownia sawdust, 5-10 parts of phosphogypsum, 4-8 parts of corn cob, 5-10 parts of pepper seed cake, 10-15 parts of mushroom dreg, 8-12 parts of wheat bran, 15-20 parts of bentonite, 10-15 parts of opoka, 2-3 parts of ferrous sulfate, 1-2 parts of manganese sulfate, 3-5 parts of humic acid, 1-2 parts of chinese tallow tree root and bark, 2-3 parts of sophora alopecuroides, 1-2 parts of stemona, 1-3 parts of Artemisia annua, 1.5-2.5 parts of ailanthus leaf, 2-3 parts of derris and 1-2 parts of Radix Euphorbiae Ebractealatae. The organic fertilizer disclosed by the invention has multiple effects of providing nutrients, improving the soil structure, preventing and treating pests and diseases and the like, can promote the crops to quickly grow, enhances the stress tolerance of the crops, can have favorable preventing and treating effects on nematodes and soil insects, reduces or avoids use of chemical pesticides, enhances the soil fertility, regulates the ecological equilibrium of soil, improves the quality of agricultural products, has no chemical residues, and implements yield increase and income increase of agricultural products.

The invention provides a biocontrol bacterial strain for preventing and treating plant diseases and its bacterial agent, belonging to the field of biological control. The strains used are Gram-negative bacteria, identified as Lysobacter enzymogenes, and the strain code is OH11. Strain OH11 has no flagella but has slippage, can produce various extracellular hydrolytic enzymes including chitinase, β-1,3-glucanase and protease, and can effectively inhibit the growth of fungi and bacteria. The antibacterial zone diameters of strain OH11 against Sclerotinia sclerotiorum and Phytophthora capsici were both greater than 22.0 mm; the antagonism against potato ring rot was stronger, and the diameter of the inhibition zone reached 50 mm. The OH11 strain was inoculated into the seed tank, cultivated to the logarithmic growth phase, and the seed liquid was connected to the production tank for cultivation. The medium used in the production tank was the same as that of the seed tank. The liquid fermentation adopts aerobic submerged fermentation and fed-batch process, the dissolved oxygen is 15%-20%, the fermentation temperature is 30°C, the fermentation time is 72h, and the initial pH value is 7.5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles, or subpackaged into solid dosage forms with peat adsorption packaging bags. The biocontrol strain OH11 can effectively control plant pathogenic fungi, bacteria, nematodes and other diseases, and the overall control effect is 50%-70%. In the greenhouse pot experiment, the control effects of OH11 on pepper blight and tomato bacterial wilt reached 83.6% and 86.4%, respectively. Strain OH11 has the characteristics of broad antibacterial spectrum, high activity, and environmental safety. In today's serious pesticide pollution, zymolysobacterium and its bacterial agent will be a good substitute.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

The invention relates to a high-throughput nano-litre micro-droplet forming and fixing method based on a microfluidic chip and a special chip and an application thereof. The method comprises the following steps: inlets of a continuous phase and a dispersed phase on the chip are respectively connected with two injection pumps through a teflon tube, the continuous phase and the dispersed phase are respectively infused into the chip under the driving action of the injection pumps, and continuous micro-droplets wrapped with single nematodes are formed in a T-shaped micro-droplet generation area; the generated micro-droplets enter in a droplet capture array and are captured in a circular droplet catcher in sequence; and after the micro-droplets are fully captured, the behaviors of the single nematodes in the droplet microenvironment are detected. The invention has the advantages that the microfluidic chip droplet technology is combined with the behavioral analysis to the single nematodes, a great amount of micro-droplets wrapped with the single nematodes are generated and fixed within the short time by using the characteristics of flexible combination and large-scale integration of various functional units on the chip, and the behavioral analysis to the high-throughput single nematodes on the microfluidic chip is realized for the first time.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

A method of cultivating crops and cover-crops, interactively and sustainably, using a novel hot water apparatus. The apparatus enables the farmer to prepare a soil bed for planting with a no-till method that: controls nematodes and soil pathogens, promotes nutrient cycling of cover crops and organic materials, kills weeds and weed seeds, and cultivates healthy soil micro flora. A low-pressure (5-60 psi) hot water (120-209 F) delivery system, characterized by a flexible mat which evenly distributes water to the underside of the mat where an attached layer of material with a high water holding capacity saturates and conducts scalding water onto the weeds. A hot water recirculation and heat exchange system reduces water demands while maintaining a high thermo charge in the mat. After soil cools, plants or seeds are planted directly through the mulch of dead weeds or cover crop. Inoculation of soil of microbes promote soil health and crop production.

The invention relates to procedures of soil disinfection to prevent and wipe out facility vegetable soil-borne diseases, operation steps are as follows: smoothing and watering the soil during the season with high temperature and good illumination to make sure relative humidity of the soil is 65%-80%, liming calcium cyan amide equably 6-100kg per 1000 m2 two days after watering, ploughing under to 30-40cm to make sure that the calcium cyan amide and the soil are mixed equably, confining the surface of the soil with transparent film and the booth or glasshouse totally for 12-20 days. The invention is mainly used for preventing and curing facility vegetable soil-borne diseases of cucumber, tomato, hot pepper, eggplant and so on, caused by pathogeny epiphyte, bacteria and wireworm. The invention prevents and cures soil-borne diseases greatly and duratively, offering vegetables nitrogen, decrease the accumulation of nitrate, accelerating plant growth. Said procedures of soil disinfection are novel to operate with easy steps, low cost, high efficiency and safety, significant economic benefit.

A strain of Trichoderma harzianum is obtained that is useful as a nematode inhibitor, fungicide and plant growth promoter. The strain has ATCC accession number PTA-3701. The strain is isolated by treating Trichoderma harzianum isolated from experimental fields of Central Institute of Medicinal and Aromatic Plants (CIMAP) Field Station with a mutagen such as ethyl methyl sulphonate, and isolating a whitish and fast growing strain of Trichoderma harzianum.

The invention discloses a composite fertilizer comprising chitin, humic acid, and middle and trace elements. The fertilizer is prepared from the raw materials of 1 part of chitin, 0.5 parts of chitosanoligosaccharide, 250 parts of urea, 450 parts of monoammonium phosphate, 100 parts of potassium chloride, 50 parts of sodium humate, 100 parts of bentonite, 10 parts of calcium-magnesium-phosphate fertilizer, 6 parts of zinc sulfate, 6 parts of borax, 4 parts of ammonium molybdate, 4 parts of manganese sulfate, 10 parts of ferrous sulfate, and 10 parts of magnesium sulfate. The fertilizer provided by the invention has the advantages that: the fertilizer has rich nutrient element; chitin, humic acid, and middle and trace elements are blended, such that the utilization rate of middle and trace elements is improved; and functions of cropping resistance, nematode control, crop disease reduction, soil improvement, crop quality improvement, high crop yield improvement are provided.