482 results about "Nematode" patented technology

The invention belongs to the field of microorganisms, and particularly relates to banana endophytic bacillus velezensis JDB15 and application thereof. The novel endophytic bacillus velezensis JDB15 is separated from banana plants, has the characteristics of efficient inhibition of Foc4, growth promotion of bananas and broad-spectrum antagonism of various pathogenic bacteria, can inhibit the morbidity of corn southern leaf blight, tomato fusarium wilt, tobacco gray mold and peach anthracnose, has the effects of inhibiting the morbidity of various pathogenic fungi, and has the advantages of high efficiency, low cost and the like. And a good broad-spectrum effect is achieved. Meanwhile, the strain can kill nematodes, has wide biocontrol bacterium activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other diseases and insect pests.

The application belongs to the field of intestinal microbiology, and discloses a new mucinophilic Akkermansia with improved obesity and glycolipid metabolism, wherein the mucinophilic Akkermansia strain is Akk-101 and AMY001. The preservation number of Akk-101 is CGMCC No. 40786, and the preservation number of AMY001 is CGMCC No. 46141. The Akk-101 and AMY001 strains in the application are different from standard strains in nucleotide consistency and physiological and biochemical indexes, have better acid-base tolerance and colonization capacity than the standard strains, can more effectively inhibit the accumulation of liver cell lipids, reduce the fat deposition in nematodes, and have the effects of weight loss and improvement of glycolipid metabolism, so that related products can be effectively developed based on the strains.

The invention discloses a pest control management system and method for forest tree breeding, and relates to the technical field of forest tree breeding and seedling raising, and the method comprises the following steps: collecting environment data and soil rhizosphere data by collecting multi-mode images of leaves and trunks of a pinus massoniana young forest and videos that pinus massoniana caterpillars gnaw leaves and cluster diffusion; constructing a disease and insect pest recognition model by using a convolutional neural network, performing definite diagnosis on pine wood nematode disease, pine caterpillars and monochamus alternatus hope, and quantifying the disease and insect pest occurrence area and population density of each sub-region; calculating a pine wood nematode disease control demand index, comparing the pine wood nematode disease control demand index with a threshold value, and triggering a corresponding control strategy; the diffusion coefficient of the pine caterpillars is calculated and compared with a risk threshold value, and a prevention and control scheme is formulated; calculating the propagation coefficient of the monochamus alternatus hope, judging the propagation risk and giving a prevention and control strategy; according to the invention, intelligent pest identification, risk quantification and control strategy closed-loop control are realized, treatment and scientific prevention and control are facilitated, and the pest control efficiency of the pinus massoniana young forest and the forest stand health level are improved.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus agave, fermentation liquor, a biological agent and application. The invention discloses a strain of bacillus tequilensis, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus tequilensis is CGMCC NO.34830. The strain of bacillus tequilensis has the advantages that the preservation number is CGMCC NO.34830; the invention also discloses a fermentation broth and a biological agent prepared according to the bacillus agave, and the bacillus agave, the fermentation broth and the biological agent provided by the invention can effectively reduce the harm of root knot nematode disease to plants and promote seed germination or crop growth, and have the advantages of high efficiency, safety, environmental protection and difficult generation of resistance.

The invention discloses a microbial agent and application thereof in agricultural production. The microbial agent is prepared by compounding seed solutions of two or more of seed solutions of Trichoderma harzianum, Trichoderma brevicompactum Tb12, Gliocladium roseum NF-06 and Trichoderma asperellum, and the seed solutions of the two or more of the Trichoderma harzianum, the Trichoderma brevicompactum Tb12, the Gliocladium roseum NF-06, the Trichoderma asperellum, the Trichoderma harzianum, the Trichoderma brevicompactum, the Gliocladium roseum NF-06 and the Trichoderma asperellum. The selected strain combination can effectively prevent and treat fungal diseases and nematode diseases and can promote plant growth. The trichoderma harzianum and the trichoderma brevicompactum Tb12 are used in a combined mode, the control effect on fusarium is obvious, the gliocladium roseum NF-06 and the trichoderma asperellum are used in a combined mode, the nematode killing effect is obvious, and the growth of rice is promoted through the trichoderma harzianum and the trichoderma asperellum.

The invention discloses application of fermented lactobacillus mucus capable of prolonging life, resisting aging and resisting oxidation, and belongs to the technical field of functional strains. The preservation numbers of the lactobacillus mucus A21224 and the lactobacillus mucus A21227 are GDMCC No: 62958 and GDMCC No: 62962 respectively, and the lactobacillus mucus A21224, the lactobacillus mucus A21227 and the lactobacillus mucus A21227 are used for The two strains can remarkably prolong the service life of nematodes, have good antioxidant effect and heat stress resistant effect, can prolong the service life of the nematodes by 40% in a strong oxidation environment, and can prolong the service life of the nematodes by 28.57% under the stress of a thermal environment. In addition, the lactobacillus mucus A21224 and the lactobacillus mucus A21227 both show an excellent anti-aging effect in the late stage of the nematodes. Meanwhile, the two strains have certain gastrointestinal fluid-resistant and cholate-resistant effects, can be colonized in intestinal tracts, have no hemolysis phenomenon and are high in safety.

The invention belongs to the technical field of biological bacterial fertilizers, and discloses a Chinese herbal medicine carrier-based multifunctional microbial bacterial fertilizer, which comprises a microbial strain group, a Chinese herbal medicine active component group, an organic matter and nutrition carrier, mineral elements and auxiliary components, and a functional synergist. Through the synergistic interaction of Chinese herbal medicine active ingredients and functional microbial flora, breeding of harmful bacteria in soil is inhibited, synthesis of acetylcholin esterase of nematodes is interfered, a continuous cropping disease occurrence chain is blocked from the source, and meanwhile, organic acid and flavonoid substances slowly released by a Chinese herbal medicine carrier are utilized to activate resistance gene expression of a plant system, so that the disease resistance of the nematodes is improved. The tolerance threshold of crops to high temperature, cold damage and diseases is enhanced; in the composite carrier, humic acid and diatomite are crosslinked through hydrogen bonds to form a stable granular structure, the cation exchange capacity and porosity of soil are effectively improved, the strong hydration capacity of polyglutamic acid is combined to lock nutrients and moisture, and development of meristem of a root system and proliferation of capillary roots are promoted.

The invention relates to the technical field of pest prevention and control, in particular to a method for treating pine wood nematode infected wood through poria cocos. The method comprises the following steps: cutting down current-year epidemic wood, and treating tree heads, tree trunks and boughs to complete peeling and tendon retaining treatment; uniformly cutting the trunk and the boughs into standard cut-logs; the method comprises the following steps: selecting fresh poria cocos sclerotium skin tissues as strains, and cutting according to an area greater than 50% of a cross section; pit pits are dug in the sunny slope; laying small branches on the bottom layer, placing the cut-logs in the pits, fixing the cut-logs with non-woven fabrics after inoculation, covering the upper layer with thin branches and leaves, and covering with soil; the two-stage biological process of mycelium planting, propagation expanding and pine wood nematode and medium insect killing is sequentially completed through a soil heat and moisture preservation environment; the propagation path of pine wood nematodes is blocked through the natural isolation effect of soil, deadwood obtained after poria cocos is subjected to biological verification and then smashed on site to return to forests or be made into fuel particles, and harmless and resource recycling of epidemic wood is achieved. According to the method, the problems of pine wood nematode propagation and epidemic wood treatment are synchronously solved.

Compounds of the formula (I) wherein Q is as defined in claim 1. Furthermore, the present invention relates to agrochemical compositions which comprise compounds of formula (I), to preparation of these compositions, and to the use of the compounds or compositions in agriculture or horticulture for combating, preventing or controlling animal pests, including arthropods and in particular insects, molluscs, nematodes or representatives of the order Acarina.

The invention relates to a composition comprising natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes. The invention further relates to methods employing natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes, preferably said composition, for protecting a plant or plant part, for improving the development and / or yield of an agricultural plant, and for protecting a soil and / or a growth substrate.

The invention belongs to the field of intestinal microbiology, and discloses a novel ackermania muciniphila capable of improving obesity and glycolipid metabolism, and the ackermania muciniphila strains are Akk-101 and AMY001. The preservation number of the Akk-101 is CGMCC (China General Microbiological Culture Collection Center) No. 40786, and the preservation number of the AMY001 is CGMCC No. 46141. The Akk-101 and AMY001 strains are different from standard strains in nucleotide consistency and physiological and biochemical indexes, have acid-base tolerance and colonization ability superior to those of the standard strains, and have the effects of more effectively inhibiting lipid accumulation of liver cells, reducing fat deposition in nematode bodies, losing weight and improving glycolipid metabolism. Therefore, related products can be effectively developed based on the strain.

The present invention provides an eco-friendly, biodegradable, controlled-release pesticidal composition, comprising particular essential oils acting in synergy, adsorbed onto a silicate mineral carrier and embedded within a polysaccharide matrix, which is effective in suppressing nematode populations and enhancing plant health and growth, and methods of use.

The invention discloses lactobacillus johnsonii A21065 capable of resisting oxidation, prolonging life and delaying Parkinson's disease as well as a product and application of the lactobacillus johnsonii A21065, and belongs to the technical field of functional strains. The preservation number of the lactobacillus johnsonii A21065 is GDMCC (China General Microbiological Culture Collection Center) No: 65767. In a caenorhabditis elegans model, the lactobacillus johnsonii A21065 shows that the lactobacillus johnsonii A21065 has better effects of improving the oxidation resistance of nematodes, resisting environmental stress and prolonging the life, also shows a good effect in in-vitro experiments of resisting artificial gastrointestinal fluid, resisting cholate and the like, meanwhile, the lactobacillus johnsonii A21065 also has an effect of improving the cognition of a Parkinson nematode model, and the lactobacillus johnsonii A21065 has a good application prospect. The invention has important significance for preventing and / or treating Parkinson's disease.

Disclosed in the present invention are a triazine derivative, a preparation method therefor and the use thereof, belonging to the technical field of drug synthesis. Specifically, disclosed in the present invention are a triazine derivative represented by general formula (I), a pharmaceutical composition containing the triazine derivative, and the use thereof in the preparation of an anthelmintic drug for humans or animals. The general structural formula (I) of the triazine derivative is shown as follows. The triazine derivative of the present invention has a significant effect in resisting zoonotic helminths such as nematodes and heartworms, and has a broad application prospect.

This disclosure relates to the field of molecules having pesticidal utility against pests in Phyla Arthropoda, Mollusca, and Nematoda, processes to produce such molecules, intermediates used in such processes, and processes of using such pesticidal compositions against such pests. The pesticidal compositions comprise (A) an oxathiazolidine derivative of formula one and (B) a mixing partner and may be used, for example, as acaricides, insecticides, miticides, molluscicides, and nematicides.

The invention discloses a green preparation method of hilsa herring roe-derived peptide, the hilsa herring roe-derived peptide and application of the hilsa herring roe-derived peptide, the hilsa herring roe-derived peptide is prepared by the following steps: cleaning and dehydrating hilsa herring roes with 10% saline water to reduce the water content of the roes, then freeze-drying and crushing to prepare freeze-dried hilsa herring roe powder, and degreasing the protein of the hilsa herring roes by a supercritical CO2 extractor to obtain the hilsa herring roe-derived peptide. Rancidity of the hilsa herring roes caused by lipid oxidation is effectively prevented; a hilsa herring egg solution is subjected to ultrasonic treatment, the protein structure of the hilsa herring eggs is uniformly damaged, and specific protease is adopted for enzymolysis, so that the enzymolysis time can be effectively shortened, the enzymolysis efficiency can be improved, and the hilsa herring egg source peptide with antioxidant activity and ACE inhibitory activity is obtained. The anti-aging test of the hilsa herring roe-derived peptide in a nematode animal model shows that the hilsa herring roe-derived bioactive peptide can obviously prolong the life of nematodes, and the average life of the nematodes can be prolonged by 33.33%. The method disclosed by the invention is of great significance in fully utilizing shad roe resources, developing functional foods and anti-aging products and realizing high-valued utilization of characteristic aquatic resources.

The invention provides a pine pollen fermentation product with anti-wrinkle and anti-aging functions as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The pine pollen extract is prepared by fermenting lactobacillus plantarum, the total polysaccharide content of the obtained pine pollen fermentation product is maximally increased by 77.3%, the FRAP total antioxidant capacity is maximally increased by 2.85 times compared with that of the unfermented pine pollen fermentation product, the anti-wrinkle effect of human skin cells can be effectively improved, and the pine pollen extract has a relatively strong proliferation effect on an ultraviolet damage model of human skin fibroblasts; the pine pollen extract has the advantages that the pine pollen extract is fermented, the elastin content is remarkably increased, the life of nematodes is effectively prolonged, lipofuscin deposition is reduced, and compared with unfermented pine pollen extract, the anti-aging capacity of the pine pollen can be effectively improved; the preparation method provided by the invention is low in price and simple in process, and the active ingredients and the efficacy of the traditional Chinese medicine composition are improved in a simplest and effective manner.

The invention provides a microbial agent for preventing and treating nematode diseases as well as a preparation method and application thereof, and relates to the technical field of organic fertilizers, the microbial agent is mainly prepared from the following raw materials in percentage by mass: 60-75% of microbial thalli; 25%-40% of an additive; wherein the microbial thalli are prepared from purpureocillium lilacinum, bacillus subtilis, brevibacillus laterosporus, bacillus mucilaginosus and lactobacillus sporogenes. Through the collaborative design of a specific strain combination and a functional additive, the stress resistance and colonization ability of the microbial inoculum in a complex soil environment can be effectively improved, meanwhile, an efficient synergistic mechanism of fungi and bacteria is established, the microbial inoculum can adapt to complex soil types, and the control effect on nematodes can be effectively improved. The invention fundamentally solves the industrial obstacles of poor environmental adaptability, short lasting period, single function and the like of biological agents, and provides a solution for large-scale application for chemical pesticide substitution.

The invention discloses a compound composition of fluopyram and dsRNA (double-stranded ribonucleic acid) and application of the compound composition in prevention and control of nematodes. The method comprises the following steps: soaking pine wood nematodes by adopting a fluopyram medicament with LC20 concentration, collecting samples at different time points respectively, and carrying out transcriptome analysis. The pesticide compound composition for effectively poisoning and killing the pine wood nematodes is obtained by comparing gene expression differences at different time points, screening out remarkably up-regulated detoxification genes BxCYP, BxCES and BxUDP, designing and synthesizing double-stranded RNA (ds BxCYP, ds BxCES and ds BxUDP) according to the detoxification genes, and compounding the double-stranded RNA with fluopyram. The pesticide compound composition has a remarkable synergistic prevention and control effect on pine wood nematodes, is expected to become a novel and efficient pine wood nematode prevention and control agent, and provides a new strategy and theoretical basis for prevention and control of pine wood nematode diseases.

The invention relates to a substituted arylthio thiazolidone derivative as well as preparation and application thereof. Specifically, the invention relates to a compound as shown in a formula (I), or an isotope-labeled compound, or an optical isomer, a geometric isomer, a tautomer or an isomer mixture, or an acceptable salt of the compound, which is used as a substituted arylthio thiazolidone derivative, and application of the compound in preparation of insecticides for pest control, the compound can achieve a better pest control effect at a lower dosage, especially for pests such as tetranychidae, microcosaridae, acaridae, tarsonidae, aleuridae and root-knot nematode, and can be used for controlling pests such as tetranychidae, microcosaridae, gall mite, tarsonidae, aleuridae and the like.

The invention discloses a brevundimonas sp. Fermentation medium and application thereof in prevention and treatment of plant root-knot nematodes. Each liter of the brevundimonas chinensis fermentation medium comprises the following components by mass or volume: 6-10 g / L of yeast powder, 16-18 g / L of peptone, 31-45 ml / L of nematode lysis buffer, 0.8-2.5 g / L of chitin and 5.6-6 g / L of yeast extract, and the pH value of the brevundimonas chinensis fermentation medium is 6.5-6.8. The application of the fermentation broth culture medium refers to the application of the obtained fermentation broth in prevention and treatment of plant root-knot nematodes. According to the present invention, with the fermentation medium, the fatality rate of the obtained fermentation broth on the plant root-knot nematode exceeds 95%, the action time does not exceed 12 h, the nematode can be rapidly killed before the larva enters the plant, and the good application prospect is provided in the plant root-knot nematode prevention and control.

The application discloses a nematicidal composition and application thereof, wherein the composition contains active ingredient A and active ingredient B, the weight ratio of the active ingredient A to the active ingredient B is 1:50-50:1, the active ingredient A is a compound shown as a formula I, and the active ingredient B is a nematicidal thiazole. The nematicidal composition is used for preventing and treating plant parasitic nematodes, has good nematicidal activity, and has little toxicity to human and animals.

The utility model provides a sweet potato nematode culture detection device, which relates to the technical field of sweet potato nematode culture and comprises a culture box, a group of movable holes I are formed in the outer surface wall of the culture box, two mounting holes are formed in one side of the outer wall of the culture box, and a bottom plate is fixedly inserted into the inner surface wall of the culture box. According to the sweet potato nematode detection device, when sweet potato nematodes need to be detected, the motor can be started, the motor drives the connecting arm and the lug block on the lower portion to move through the crankshaft, the filter sieve vibrates up and down to shake off the sweet potato nematodes into the collecting box on the lower portion, and a user can take out the sweet potato nematodes only by pulling out the collecting box; the sweet potato nematodes can be conveniently and rapidly collected, the complexity and time cost of manual operation are reduced, possible damage to the sweet potato nematodes caused by a traditional flotation method and the like is avoided, the integrity and health state of the nematodes are guaranteed, and subsequent experiments or applications are facilitated.

The invention relates to a multi-channel controllable pine wood nematode collection culture dish and a culture and collection method, and belongs to the technical field of pine wood nematode culture.The multi-channel controllable pine wood nematode collection culture dish comprises a culture dish body, the culture dish body comprises a culture dish cover and a culture dish body, the culture dish cover is provided with three sampling holes, sampling pipes are installed at the sampling holes, and the three sampling holes are communicated with the culture dish body. The three sampling tubes are annularly arranged along the center of the culture dish cover. According to the method, the natural enrichment phenomenon is utilized, the sampling pipe and the sample leaking hopper are matched, water drops containing the nematodes are collected through gravity, compared with a traditional filtering centrifugal collection method, the traditional time-consuming filtering step is omitted, the nematodes can be used after being extracted, the operation process is remarkably simplified, the nematode collection time is greatly shortened, the working efficiency is improved, and the method is suitable for popularization and application. And the three sampling pipes synchronously collect and continuously collect after the sampling pipes are replaced, so that the resource utilization rate is increased.

The invention relates to a composition comprising natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes. The invention further relates to methods employing natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes, preferably said composition, for protecting a plant or plant part, for improving the development and / or yield of an agricultural plant, and for protecting a soil and / or a growth substrate.

The invention relates to the technical field of chemical toxicology, in particular to an evaluation method for comprehensively analyzing the immune induction effect of a plasticizer by using caenorhabditis elegans, according to the evaluation method, a mother worm breeding mode is changed to replace a lysis solution to synchronize nematodes, experimental interference is reduced, the result accuracy is improved, monitoring indexes are newly increased, intestinal injuries and in-vivo bacterial indexes are increased, and the evaluation method is suitable for comprehensive analysis of the immune induction effect of the plasticizer. The evaluation is more accurate and reliable by combining oxidative stress indexes and performing two-dimensional comprehensive evaluation from an intestinal immune defense line and oxidative stress, and by drawing a multi-index monitoring shape graph, adopting a comprehensive biomarker response index analysis method and associating immune-related representative indexes, the evaluation accuracy is improved. According to the method, the change of each index and the immunity intensity on the caenorhabditis elegans under different exposure conditions are intuitively presented, the immune defense effect of the caenorhabditis elegans under the stress of the plasticizer can be quickly and effectively evaluated without transcriptome and metabonomics, and efficient evaluation is realized.

The invention discloses an application of nitrone compounds in preparation of related products for improving athletic ability. The nitrone compound disclosed by the invention has a structure shown in a general formula (I), and experimental results show that the nitrone compound disclosed by the invention can be used for increasing the exercise capability of nematodes of different age groups, including the body swing frequency and the pharyngeal pumping rate.

This invention discloses a pesticide composition for controlling guava nematodes and its preparation method, belonging to the field of pesticide technology. By weight, the raw materials of the pesticide composition include the following components: 8-12 parts of neem seed extract, 8-12 parts of pyrethrum extract, 5-8 parts of tongkat ali extract, 5-8 parts of croton fruit peel extract, 5-8 parts of guava leaf extract, 20-25 parts of compound microbial agent, 3-5 parts of sodium lignosulfonate, 5-8 parts of xanthan gum, 4-6 parts of trehalose, and 80-100 parts of water. The above components work synergistically to achieve excellent control of guava nematodes.

To provide a process for forming arylcyclopropylcarboxylic acids useful for forming molecules having insecticidal applications against pests in the phyla Arthropoda, Mollusca, and Nematoda.SOLUTION: There is provided an enantiomerically enriched preparation of the compound (R2, R4) - 3 - (3, 5-bis (trifluoromethyl) phenyl) - 2, 2-dichlorocyclopropane-1-carboxylic acid in a heterogeneous S3a, wherein CF3 and R3 are; R2, and R5 are H; and R7 and R8 are Cl; wherein the enantiomeric excess of the (R, R) - enantiomer of the is greater than 80%, greater than 90% or greater than 95%. 1R 3R S3a. Also provided are pesticidal formulations comprising said enantiomerically enriched preparations.SELECTED DRAWING: None