604 results about "Nematode" patented technology

The invention belongs to the field of microorganisms, and particularly relates to banana endophytic bacillus velezensis JDB15 and application thereof. The novel endophytic bacillus velezensis JDB15 is separated from banana plants, has the characteristics of efficient inhibition of Foc4, growth promotion of bananas and broad-spectrum antagonism of various pathogenic bacteria, can inhibit the morbidity of corn southern leaf blight, tomato fusarium wilt, tobacco gray mold and peach anthracnose, has the effects of inhibiting the morbidity of various pathogenic fungi, and has the advantages of high efficiency, low cost and the like. And a good broad-spectrum effect is achieved. Meanwhile, the strain can kill nematodes, has wide biocontrol bacterium activity, can be developed deeply subsequently, and provides a sustainable and green solution for prevention and treatment of banana wilt and other diseases and insect pests.

The application belongs to the field of intestinal microbiology, and discloses a new mucinophilic Akkermansia with improved obesity and glycolipid metabolism, wherein the mucinophilic Akkermansia strain is Akk-101 and AMY001. The preservation number of Akk-101 is CGMCC No. 40786, and the preservation number of AMY001 is CGMCC No. 46141. The Akk-101 and AMY001 strains in the application are different from standard strains in nucleotide consistency and physiological and biochemical indexes, have better acid-base tolerance and colonization capacity than the standard strains, can more effectively inhibit the accumulation of liver cell lipids, reduce the fat deposition in nematodes, and have the effects of weight loss and improvement of glycolipid metabolism, so that related products can be effectively developed based on the strains.

The invention discloses a pest control management system and method for forest tree breeding, and relates to the technical field of forest tree breeding and seedling raising, and the method comprises the following steps: collecting environment data and soil rhizosphere data by collecting multi-mode images of leaves and trunks of a pinus massoniana young forest and videos that pinus massoniana caterpillars gnaw leaves and cluster diffusion; constructing a disease and insect pest recognition model by using a convolutional neural network, performing definite diagnosis on pine wood nematode disease, pine caterpillars and monochamus alternatus hope, and quantifying the disease and insect pest occurrence area and population density of each sub-region; calculating a pine wood nematode disease control demand index, comparing the pine wood nematode disease control demand index with a threshold value, and triggering a corresponding control strategy; the diffusion coefficient of the pine caterpillars is calculated and compared with a risk threshold value, and a prevention and control scheme is formulated; calculating the propagation coefficient of the monochamus alternatus hope, judging the propagation risk and giving a prevention and control strategy; according to the invention, intelligent pest identification, risk quantification and control strategy closed-loop control are realized, treatment and scientific prevention and control are facilitated, and the pest control efficiency of the pinus massoniana young forest and the forest stand health level are improved.

The invention discloses a forest pine nematode disease identification and positioning method based on deep learning, and the method comprises the steps: constructing a multi-modal data set through the fusion of LiDAR point cloud, image data and time sequence information, and employing the advantages of a convolutional neural network (CNN), a graph neural network (GNN) and a long short-term memory network (LSTM) in the method, thereby achieving the recognition and positioning of the forest pine nematode disease. According to the method, high-precision capture and analysis of tree geometric structure information and time change conditions are realized, and a scientific basis can be provided for tracking the origin of pathogens by determining an initial infection source, so that a more effective control strategy is formulated; by generating the infection probability graph and predicting the transmission path of the pine nematode disease, the transmission of the pine nematode disease can be predicted in advance, and the source of the pine nematode disease can be found; compared with the prior art, prevention measures can be taken more quickly, the risk of pine nematode disease diffusion is reduced, and the efficiency and accuracy of forest pine nematode disease monitoring are improved.

The invention discloses a method for inducing nematode-trapping fungus predatory organ formation, and belongs to the field of applied microorganisms. The method comprises the following specific steps: culturing activated nematode-trapping fungi until spores are generated; washing off the cultured spores, and diluting to obtain a spore suspension with the concentration of 30000-60000 spores / mL; coating the spore suspension into an agar culture medium paved with glass paper, uniformly coating the spore suspension, culturing for 28-36 hours at the temperature of 25-28 DEG C, adding a mixed solution of a predatory organ inducer and water, and continuously culturing for 36-60 hours at the temperature of 25-28 DEG C, so that the nematode-trapping fungi can generate a large number of predatory organs; the predatory organ inducer is one of oleic acid, linoleic acid or pregnenolone. It is found for the first time that a large number of predatory organs can be generated when pregnenolone, oleic acid or linoleic acid is used for inducing nematode-trapping fungi.

The invention provides a rapid detection kit for bursaphelenchus xylophilus and a method and application thereof.The detection kit comprises a fluorescent RAA dry powder reagent premixed with primer probes, the primer probes for rapidly detecting the bursaphelenchus xylophilus based on RAA are designed and screened, and a primer and probe set for specifically amplifying the ITS gene of the bursaphelenchus xylophilus is obtained; no cross reaction with the bursaphelenchus mucronatus is caused; meanwhile, the kit comprises a lysis solution for rapidly extracting the DNA of the pine wood nematode suspension, and the lysis solution contains quaternary ammonium salt, isopropanol and DMSO, so that the DNA extraction quality and efficiency are remarkably improved. The bursaphelenchus xylophilus detection kit provided by the invention is strong in detection specificity, high in sensitivity, simple to operate and portable, and can rapidly detect bursaphelenchus xylophilus within 30 minutes under field conditions.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus agave, fermentation liquor, a biological agent and application. The invention discloses a strain of bacillus tequilensis, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus tequilensis is CGMCC NO.34830. The strain of bacillus tequilensis has the advantages that the preservation number is CGMCC NO.34830; the invention also discloses a fermentation broth and a biological agent prepared according to the bacillus agave, and the bacillus agave, the fermentation broth and the biological agent provided by the invention can effectively reduce the harm of root knot nematode disease to plants and promote seed germination or crop growth, and have the advantages of high efficiency, safety, environmental protection and difficult generation of resistance.

The invention relates to the field of agricultural protection preparations, in particular to preparation of gold nanoclusters and application of the gold nanoclusters to prevention and treatment of plant parasitic nematodes. The invention provides a preparation method of a gold nanocluster, which comprises the following steps: preparing by using HAuCl4 and L-His to obtain an L-type histidine modified gold nanocluster; the preparation method comprises the steps that a HAuCl4 aqueous solution and an L-His aqueous solution are evenly mixed and then incubated, an obtained reaction solution containing L-His-AuNCs is centrifuged and then subjected to aftertreatment such as ultrafiltration, and the gold nanocluster is obtained. The gold nanocluster can be used for preventing and treating pine wood nematodes.

The invention relates to the technical field of biology, in particular to a Korean pine control-based method for researching the resistance mechanism of larch to pine wood nematodes, which comprises the following steps: S1, extracting pine wood nematodes: separating to-be-tested nematodes from naturally infected larch by using a funnel; s2, pine wood nematode culture: utilizing botrytis cinerea to co-culture pine wood nematode as a test raw material; s3, selecting tree species: selecting twigs, boughs and trunks of healthy larch with tree ages of 1 year, 2 years and 3 years respectively, and selecting Korean pine with the same requirements as a contrast; s4, sampling tree species: sampling larch and Korean pine inoculated for 1 day, 3 days, 5 days, 7 days, 9 days, 14 days and 21 days; by analyzing the chemical components of the Korean pine and the larch in different stages before, during and after the bursaphelenchus xylophilus infection, the purpose of researching the pathogenesis of the Korean pine and the larch by local pine nematodes in Liaoning is achieved, so that the purpose of effectively preventing the bursaphelenchus xylophilus is achieved.

The invention discloses a microbial agent and application thereof in agricultural production. The microbial agent is prepared by compounding seed solutions of two or more of seed solutions of Trichoderma harzianum, Trichoderma brevicompactum Tb12, Gliocladium roseum NF-06 and Trichoderma asperellum, and the seed solutions of the two or more of the Trichoderma harzianum, the Trichoderma brevicompactum Tb12, the Gliocladium roseum NF-06, the Trichoderma asperellum, the Trichoderma harzianum, the Trichoderma brevicompactum, the Gliocladium roseum NF-06 and the Trichoderma asperellum. The selected strain combination can effectively prevent and treat fungal diseases and nematode diseases and can promote plant growth. The trichoderma harzianum and the trichoderma brevicompactum Tb12 are used in a combined mode, the control effect on fusarium is obvious, the gliocladium roseum NF-06 and the trichoderma asperellum are used in a combined mode, the nematode killing effect is obvious, and the growth of rice is promoted through the trichoderma harzianum and the trichoderma asperellum.

The invention discloses a culture method for improving the reproductive capacity of indigenous nematodes and application of the culture method, and belongs to the technical field of indigenous nematode culture. According to the present invention, the Rosselomyces aquimaris L-3 strain is the indigenous nematode intestinal tract strain, is the new strain, can enrich the nutrition source of the indigenous nematode, can significantly improve the egg laying amount of the nematode fed with the strain in the early stage of the breeding period, and can improve the egg laying amount by 36.3% compared with the currently common E. coli OP50 bacteria, and can not produce any harm to the indigenous nematode; the provided culture method is convenient and efficient, the breeding speed of indigenous nematodes can be remarkably increased under the laboratory environment condition by adopting the culture method provided by the invention, and compared with the currently common E. coli OP50 bacteria, the extremely remarkable difference can be achieved in the second breeding day, the necessary nematode number can be provided for short-period experiments, and the related research process can be accelerated.

The present disclosure relates to the field of molecules having pesticidal utility on pests in Phyla Arthropoda, Mollusca and Nematoda, methods of producing such molecules, intermediates used in such methods, and methods of using such pesticidal compositions against such pests. These pesticidal compositions can be used, for example, as acaricides, insecticides, acaricides, molluscicides, and nematicides.

The invention discloses application of fermented lactobacillus mucus capable of prolonging life, resisting aging and resisting oxidation, and belongs to the technical field of functional strains. The preservation numbers of the lactobacillus mucus A21224 and the lactobacillus mucus A21227 are GDMCC No: 62958 and GDMCC No: 62962 respectively, and the lactobacillus mucus A21224, the lactobacillus mucus A21227 and the lactobacillus mucus A21227 are used for The two strains can remarkably prolong the service life of nematodes, have good antioxidant effect and heat stress resistant effect, can prolong the service life of the nematodes by 40% in a strong oxidation environment, and can prolong the service life of the nematodes by 28.57% under the stress of a thermal environment. In addition, the lactobacillus mucus A21224 and the lactobacillus mucus A21227 both show an excellent anti-aging effect in the late stage of the nematodes. Meanwhile, the two strains have certain gastrointestinal fluid-resistant and cholate-resistant effects, can be colonized in intestinal tracts, have no hemolysis phenomenon and are high in safety.

The invention belongs to the technical field of biological bacterial fertilizers, and discloses a Chinese herbal medicine carrier-based multifunctional microbial bacterial fertilizer, which comprises a microbial strain group, a Chinese herbal medicine active component group, an organic matter and nutrition carrier, mineral elements and auxiliary components, and a functional synergist. Through the synergistic interaction of Chinese herbal medicine active ingredients and functional microbial flora, breeding of harmful bacteria in soil is inhibited, synthesis of acetylcholin esterase of nematodes is interfered, a continuous cropping disease occurrence chain is blocked from the source, and meanwhile, organic acid and flavonoid substances slowly released by a Chinese herbal medicine carrier are utilized to activate resistance gene expression of a plant system, so that the disease resistance of the nematodes is improved. The tolerance threshold of crops to high temperature, cold damage and diseases is enhanced; in the composite carrier, humic acid and diatomite are crosslinked through hydrogen bonds to form a stable granular structure, the cation exchange capacity and porosity of soil are effectively improved, the strong hydration capacity of polyglutamic acid is combined to lock nutrients and moisture, and development of meristem of a root system and proliferation of capillary roots are promoted.

The invention relates to preparation and application of a trifluoroethyl thiophenylpyrimidinamine derivative. Specifically, the invention relates to a compound as shown in a formula (I), or an isotope labeled compound thereof, or an optical isomer, a geometric isomer, a tautomer or an isomer mixture thereof, or a pharmaceutically acceptable salt thereof, and application of the compound in preparation of insecticides for pest control. The compound can achieve a better pest control effect at a lower dosage, especially for pests such as tetranychidae, microcosaridae, acaridae, tarsonidae, aleuridae and root-knot nematode, and can be used for controlling pests such as tetranychidae, microcosaridae, gall mite, tarsonidae, aleuridae and the like. # imgabs0 #

The invention discloses an application of an Ace9 factor in enhancing the activity of killing plant parasitic nematodes by multiple bacilli, and belongs to the technical field of microbial pesticides. It is found for the first time that the nematicidal activity of bacillus fermentation liquid supernatant can be improved by 50% or above through Ace9 factor culture, and therefore the Ace9 factor has the potential of being developed into a microbial pesticide synergist, and the control effect of microbial pesticide on parasitic nematodes can be greatly improved.

The invention provides application of a pseudomonas chlororaphis strain Rhs-P27 in prevention and treatment of pine wood nematode diseases, and relates to the technical field of agricultural environmental microorganisms. In particular to a Pseudomonas chlororaphis strain Rhs-P27 separated from rhizosphere soil of Chinese pines and application of the Pseudomonas chlororaphis strain Rhs-P27 in prevention and treatment of pine wood nematode diseases. The strain is preserved in the China Center for Type Culture Collection in Wuhan University on December 18, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) 20242846. The in-vitro poisoning activity of the strain Rhs-P27 on pine wood nematodes can reach 100% within 24 hours; a fermentation filtrate and a zymophyte suspension of the strain Rhs-P27 have a remarkable inhibition effect on the propagation of the pine wood nematodes; the prevention effect of a zymophyte suspension of the strain Rhs-P27 on the pine wood nematode disease of biennial pinus tabulaeformis potted seedlings can reach 45%, and the density of pine wood nematode in pine trees can be remarkably reduced; the pseudomonas chlororaphis strain Rhs-P27 disclosed by the invention has a good application prospect in the field of pollution-free prevention and treatment of pine wood nematode diseases.

The invention discloses a five-fold real-time fluorescence quantitative PCR detection kit for simultaneously detecting five parasites and its special primers and probes, belonging to the field of biological detection technology. The kit provided by the invention can be used to simultaneously detect Cryptosporidium parvum, Strongyloides stercoralis, Trypanosoma cruzi, Theileria fasciolae and Echinococcus granulosus in the same system, and has the characteristics of qualitative and quantitative detection, high sensitivity, strong specificity, good repeatability and good stability.

The invention relates to the technical field of pest prevention and control, in particular to a method for treating pine wood nematode infected wood through poria cocos. The method comprises the following steps: cutting down current-year epidemic wood, and treating tree heads, tree trunks and boughs to complete peeling and tendon retaining treatment; uniformly cutting the trunk and the boughs into standard cut-logs; the method comprises the following steps: selecting fresh poria cocos sclerotium skin tissues as strains, and cutting according to an area greater than 50% of a cross section; pit pits are dug in the sunny slope; laying small branches on the bottom layer, placing the cut-logs in the pits, fixing the cut-logs with non-woven fabrics after inoculation, covering the upper layer with thin branches and leaves, and covering with soil; the two-stage biological process of mycelium planting, propagation expanding and pine wood nematode and medium insect killing is sequentially completed through a soil heat and moisture preservation environment; the propagation path of pine wood nematodes is blocked through the natural isolation effect of soil, deadwood obtained after poria cocos is subjected to biological verification and then smashed on site to return to forests or be made into fuel particles, and harmless and resource recycling of epidemic wood is achieved. According to the method, the problems of pine wood nematode propagation and epidemic wood treatment are synchronously solved.

Compounds of the formula (I) wherein Q is as defined in claim 1. Furthermore, the present invention relates to agrochemical compositions which comprise compounds of formula (I), to preparation of these compositions, and to the use of the compounds or compositions in agriculture or horticulture for combating, preventing or controlling animal pests, including arthropods and in particular insects, molluscs, nematodes or representatives of the order Acarina.

The invention relates to a composition comprising natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes. The invention further relates to methods employing natamycin and at least one insecticide that interferes with the nervous system and / or muscular system of insects and nematodes, preferably said composition, for protecting a plant or plant part, for improving the development and / or yield of an agricultural plant, and for protecting a soil and / or a growth substrate.

The invention belongs to the field of microbial preparations, and discloses a microbial stabilizer which comprises non-bacillus or a zymophyte solution thereof and a chemical stabilizer, and the non-bacillus comprises at least one of serratia nematophila and acinetobacter calcoaceticus. The invention also discloses a preparation method of the microbial stabilizer and an application of the microbial stabilizer in prolonging the shelf life of a microbial agent. The research on the microbial stabilizer containing non-bacillus discovers that the microbial stabilizer formed by proper matching of the non-bacillus and the chemical stabilizer can keep the stability of the concentration of viable bacteria for a long time, prolong the shelf life of microbial products and reduce the requirements of frequent production and storage; therefore, the production cost is directly reduced, the economic benefit is improved, and the method can be used in the technical field of microbial preparations.

The invention belongs to the field of intestinal microbiology, and discloses a novel ackermania muciniphila capable of improving obesity and glycolipid metabolism, and the ackermania muciniphila strains are Akk-101 and AMY001. The preservation number of the Akk-101 is CGMCC (China General Microbiological Culture Collection Center) No. 40786, and the preservation number of the AMY001 is CGMCC No. 46141. The Akk-101 and AMY001 strains are different from standard strains in nucleotide consistency and physiological and biochemical indexes, have acid-base tolerance and colonization ability superior to those of the standard strains, and have the effects of more effectively inhibiting lipid accumulation of liver cells, reducing fat deposition in nematode bodies, losing weight and improving glycolipid metabolism. Therefore, related products can be effectively developed based on the strain.

The present invention provides an eco-friendly, biodegradable, controlled-release pesticidal composition, comprising particular essential oils acting in synergy, adsorbed onto a silicate mineral carrier and embedded within a polysaccharide matrix, which is effective in suppressing nematode populations and enhancing plant health and growth, and methods of use.

The invention discloses lactobacillus johnsonii A21065 capable of resisting oxidation, prolonging life and delaying Parkinson's disease as well as a product and application of the lactobacillus johnsonii A21065, and belongs to the technical field of functional strains. The preservation number of the lactobacillus johnsonii A21065 is GDMCC (China General Microbiological Culture Collection Center) No: 65767. In a caenorhabditis elegans model, the lactobacillus johnsonii A21065 shows that the lactobacillus johnsonii A21065 has better effects of improving the oxidation resistance of nematodes, resisting environmental stress and prolonging the life, also shows a good effect in in-vitro experiments of resisting artificial gastrointestinal fluid, resisting cholate and the like, meanwhile, the lactobacillus johnsonii A21065 also has an effect of improving the cognition of a Parkinson nematode model, and the lactobacillus johnsonii A21065 has a good application prospect. The invention has important significance for preventing and / or treating Parkinson's disease.

The invention provides application of a bacillus subtilis strain Rhs-L31 in prevention and treatment of pine wood nematode disease, and relates to an agricultural environment microbial technology. In particular relates to a bacillus friaricola strain Rhs-L31 which is separated from rhizosphere soil of Chinese pines and application of the bacillus friaricola strain Rhs-L31 in prevention and treatment of pine wood nematode diseases. The strain is preserved in the China Center for Type Culture Collection in Wuhan University on December 25, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) 20242847. The in-vitro poisoning activity of the strain Rhs-L31 on pine wood nematodes can reach 100% within 24 hours; a fermentation filtrate and a zymophyte suspension of the strain Rhs-L31 have a remarkable inhibition effect on the propagation of the pine wood nematodes; the prevention and treatment effect of a zymophyte suspension of the strain Rhs-L31 on the pine wood nematode disease of the Chinese pine potted seedlings can reach 50%-72.5%; the bacillus subtilis strain Rhs-L31 disclosed by the invention has a good application prospect in the field of pollution-free prevention and treatment of pine wood nematode diseases.

The invention relates to the technical field of agricultural microorganisms, in particular to bacillus isothermal layer R6 for preventing and treating pine wood nematode disease and application thereof. The bacillus isothermal layer R6 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO. 32275. The strain has the activity of efficiently inhibiting and killing the pine wood nematodes, the fatality rate of fermentation liquor of the strain on the pine wood nematodes can reach 90% or above when OD600 is 0.5-2, and the disease index of pine tree seedlings infected with the pine wood nematode disease can be effectively reduced.

Disclosed in the present invention are a triazine derivative, a preparation method therefor and the use thereof, belonging to the technical field of drug synthesis. Specifically, disclosed in the present invention are a triazine derivative represented by general formula (I), a pharmaceutical composition containing the triazine derivative, and the use thereof in the preparation of an anthelmintic drug for humans or animals. The general structural formula (I) of the triazine derivative is shown as follows. The triazine derivative of the present invention has a significant effect in resisting zoonotic helminths such as nematodes and heartworms, and has a broad application prospect.

The invention discloses a double Taqman detection probe primer group for trichina and sarcocystis and application of the double Taqman detection probe primer group. Relates to the technical field of parasite detection. The invention provides a detection probe primer group, a kit and application. According to the detection method established by the invention, an amplification curve has a good linear relationship; the sensitivity is high; the specificity is high; dNA detection on 12 parasites such as toxoplasma gondii, ascaris suum, cryptosporidium and pulmonary nematode does not have an amplification curve; agarose gel electrophoresis shows that the molecular mass of the obtained PCR product strip accords with expectation. The kit has the characteristics of simplicity in operation, high specificity and high sensitivity, can quickly and efficiently detect trichina and sarcocystis, and has guiding significance in quick laboratory diagnosis of infection of the trichina and sarcocystis.

The present invention discloses a fermentation and cultivation device for a nematode-resistant microbial inoculant, which relates to the technical field of fermentation and cultivation of microbial inoculants. It includes a fermentation housing. A first feeding pipe is fixedly communicated with the top of the fermentation housing, and a second feeding pipe is fixedly communicated with the top of the fermentation housing. An intermittent feeding assembly is arranged on the top of the fermentation housing. The intermittent feeding assembly includes a fixing frame fixedly connected to the top of the fermentation housing. The lower surface of the top of the fixing frame is rotatably connected with a rotating housing. Through the setting of the intermittent assembly, it plays a role in supplementing carbon sources in the nematode-resistant microbial inoculant, and at the same time achieves the purpose of quantitative feeding, avoids the problem of opening the lid for feeding, and realizes the purpose of automatic batch feeding. After the feeding, the staff has enough time to observe the concentration after feeding. At the same time, the intermittent feeding of carbon sources avoids the waste caused by a one-time input, and avoids the inhibition of the growth of the nematode-resistant microbial inoculant due to excessive carbon sources.