157 results about "Host plants" patented technology

The invention discloses a method for inoculating cistanche with atriplex canescens in the technical field of plant cultivation. The method comprises the following steps of host plant selection and cultivation, cistanche seed treatment, inoculation, cultivation management, harvesting and processing. The method has the advantages that the parasitic success rate is increased and can reach 80% or above generally; the atriplex canescens grows well, sufficient nutrients are provided for the cistanche deserticola, and the yield of the cistanche deserticola is remarkably increased and is increased by 30%-50% compared with a traditional method. And 3, the ecological benefit is remarkable: the planting of the atriplex canescens is beneficial to improving the soil structure and the ecological environment, and meanwhile, the growth of the cistanche deserticola also provides protection for the atriplex canescens, so that the mutualism of the atriplex canescens and the cistanche deserticola is realized, the vegetation coverage rate of a desertification region is enhanced, and the soil structure and the ecological environment are improved. And 4, the economic benefits are considerable, large-scale planting can be realized, a new way is provided for increasing income of farmers, and the method has good market prospects and economic benefits.

The invention discloses a botanical attractant for thrips hawaiiensis in a banana field and application of the botanical attractant. According to the application, alpha-pinene, caryophyllene, DL-limonene, beta-pinene and D-limonene which have a remarkable attracting effect on thrips hawaiiensis are separated and identified from banana flower buds which are host plants of the thrips hawaiiensis, and effective concentrations of the compounds with the attracting effect on the thrips hawaiiensis are screened out; the five compounds are mixed according to a certain volume ratio to prepare the thrips hawaiiensis luring composition. Indoor and outdoor experiments prove that the trapping composition provided by the invention has remarkable trapping activity on thrips hawaiiensis in a banana field. The attractant provided by the invention is derived from plants, is simple to prepare, is environment-friendly, is non-toxic to people and livestock, and has a good popularization and application prospect.

The invention discloses a Guangxi strigose hydrangea juvenile tea gall mite comprehensive control method based on ecological regulation and control, and belongs to the technical field of ecological planting of strigose hydrangea juvenile tea. The method comprises the steps that lemongrass is planted on the periphery to form a smell barrier, purple perilla is arranged in the middle, Guangxi strigose hydrangea juvenile tea and sugar oranges are planted at intervals in a core area, and a composite rhombic layout is formed; chinaroot greenbrier is planted in the interplanting zone at intervals to serve as gall mite trapping plants, and matrine or azadirachtin botanical pesticide is sprayed to the chinaroot greenbrier regularly; a composite substrate containing 60-65% of loess, 20-25% of bagasse biochar, 10-12% of vermiculite and 3-5% of sodium alginate-attapulgite clay composite water-retention slow-release particles is adopted to form a water-retention breathable layer with a slow-release function; and spreading wormwood seeds around the host plants to inhibit survival of overwintering adults of the gall mites. The method has good ecological benefits and insect pest prevention and control efficiency, effectively improves the soil microenvironment and is suitable for sweet tea planting in subtropical hilly areas such as Guangxi.

The invention discloses a VdNOB1 gene as well as an anti-pathogenic bacterium target gene segment, an interference vector and disease-resistant application thereof. According to the invention, the VdNOB1 gene is subjected to knockout mutation, so that the infection pathogenicity of verticillium dahliae to host plants can be obviously reduced. The invention further provides a verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment, the nucleotide sequence of the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment is shown as SEQ ID No.4, and the pathogenicity of verticillium dahliae to host plants can be remarkably reduced after plants are transformed by an RNA interference vector constructed by the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment. The invention further provides an RNA (Ribonucleic Acid) interference vector containing the VdNOB1 gene or an anti-pathogenic bacterium target gene fragment thereof. The verticillium dahliae VdNOB1 gene or the anti-pathogenic-bacterium target gene segment and the RNA interference vector thereof have application prospects in the aspects of improving the resistance of crops to diseases caused by verticillium dahliae, cultivating new varieties of verticillium dahliae-resistant plants and the like.

The present invention discloses the use of a gibberellin-deficient mutant in promoting spore production in arbuscular mycorrhizal fungi. The present invention utilizes the hairy roots of the gibberellin-deficient mutant as a host plant to inoculate the arbuscular mycorrhizal fungi, significantly increasing the spore production of the arbuscular mycorrhizal fungi. The hairy roots of the gibberellin-deficient mutant can significantly increase the spore production of the arbuscular mycorrhizal fungi under both normal pH conditions and acidic conditions. The gibberellin-deficient mutant can be widely used to promote spore production in arbuscular mycorrhizal fungi. The present invention also provides a preparation and method for promoting spore production in arbuscular mycorrhizal fungi. The preparation and method can propagate the arbuscular mycorrhizal fungi under sterile conditions with high propagation efficiency, significantly increase the spore production of the arbuscular mycorrhizal fungi, are simple to operate, low in cost, and have a short cycle time, thus having broad prospects for promotion and application.

The invention relates to the technical field of biology, and discloses an FPSE09041 protein and an application of a coding gene of the FPSE09041 protein in reducing pathogenicity of fusarium pseudograminearum. The amino acid sequence of the FPSE09041 protein is as shown in SEQ ID NO. 2, and the coding gene of the FPSE09041 protein is as shown in SEQ ID NO. 1. By knocking out the FPSE09041 gene, the expression of the FPSE09041 protein is inhibited, and the pathogenicity of the fusarium pseudograminearum to host plants is reduced. The research finds that the FPSE09041 is an indispensable key factor for pathogenicity of the fusarium pseudograminearum, and the survival and infection capabilities of pathogenic bacteria can be obviously weakened due to function deficiency of the FPSE09041. Therefore, the FPSE09041 protein and the coding gene thereof can be used as ideal molecular targets for developing novel, efficient and high-specificity antifungal agents, and a new technical path is provided for green prevention and control of wheat stem rot.

The invention provides a botanical fungicide of a chaulmoogra seed extract, which takes sub-chaulmoogra seed oleic acid as a marker, and application of the botanical fungicide in preparation of medicines for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The hydnocarpus anthelmintica extract is obtained by extracting hydnocarpus anthelmintica seeds with a plurality of different types of organic solvents such as ethanol, petroleum ether and the like and treating the seeds by a physical method, and the hydnocarpus anthelmintica extract contains sub-hydnocarpus anthelmintica oleic acid as markers in different proportions. The extract has the effects of inhibiting conidium germination, bud tube growth or appressorium formation of fungi and oomycetes, effectively preventing pathogenic bacteria from infecting host plants and preventing and treating important plant diseases such as rice blast, mango anthracnose, potato late blight and rice false smut.

The invention belongs to the technical field of gene engineering, and relates to an apple tree valsa ceratosperma virulence gene and application and dsRNA thereof. The invention discloses an apple tree valsa ceratosperma virulence gene VmNRPS25, the CDS region sequence of the apple tree valsa ceratosperma virulence gene VmNRPS25 is shown as SEQ ID NO: 1, the expression of the apple tree valsa ceratosperma virulence gene VmNRPS25 in V.mali is inhibited, the biomass of V.mali in a host plant is reduced, and the virulence of V.mali is weakened. The invention further constructs dsRNA on the basis of the valsa mali virulence gene VmNRPS25, and the dsRNA induces silencing of the valsa mali virulence gene VmNRPS25 in V.mali through an exogenous spraying method, and can be used for prevention and treatment of V.mali and pathogenic plant diseases thereof in host plants.

The invention discloses a strain NJSH56 for promoting plant growth and application thereof, the strain NJSH56 is identified as bacillus pumilus, the strain NJSH56 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation time is November 14, 2024, and the strain preservation number is CGMCC NO.32614. The strain NJSH56 can promote plant growth. The dendrobium officinale endophytic growth-promoting strain NJSH56 for promoting plant growth is obtained through separation and screening, growth of dendrobium officinale tissue culture seedlings can be effectively promoted, and the fresh weight, the rooting number and the leaf number are increased; greenhouse tests show that the number of new buds of dendrobium officinale plants in a strain NJSH56 treatment group reaches 4.67 and is obviously higher than that of a control group; meanwhile, the NJSH56 is used for treating the non-host plant Chinese cabbage, so that the growth of the overground part of the Chinese cabbage can be remarkably promoted, and the biomass accumulation of the Chinese cabbage is improved.

This invention discloses a deep learning-based method for predicting the phenological synchronization of specialized butterflies and their host plants, belonging to the interdisciplinary field of biodiversity conservation, ecological prediction, and deep learning technology. The method includes: collecting environmental climate variables, butterfly occurrence data, and phenological time-series data of their host plants; preprocessing the data to create training and validation sets; constructing a dual-label system with the day of year (DOY) of butterfly occurrence peaks (e.g., emergence peak, egg-laying peak) as the primary label and the normalized value of synchronization deviation as the secondary label; building a deep learning model with ecological weights, including an embedding layer, an LSTM layer, and a fusion layer; inputting a vector concatenated from daily or weekly climate time-series features and ecological dynamic features; and outputting the primary and secondary labels through a dual-output layer; designing a hybrid loss function that integrates the main regression loss, ecological synchronization constraint loss, and small-sample regularization loss; training the model using the Adam optimizer and implementing an early stopping mechanism; and validating and evaluating the model using regression accuracy and ecological synchronization accuracy indicators. The method includes modules for data acquisition, preprocessing, label construction, model training, prediction evaluation, and storage. This invention improves the accuracy of phenological synchronous prediction by embedding ecological dynamic feature weights and dual-label constraints, adapts to small sample scenarios, and provides technical support for the monitoring and protection management of rare butterfly species (such as the Golden Birdwing and the Chinese Tiger Swallowtail).

The invention relates to the technical field of citrus insect pest prevention and control, and discloses a comprehensive ecological prevention and control method for diaphorina citri. The method comprises the following steps: acquiring current insect pest state information based on real-time monitoring data of a citrus planting area; extracting diaphorina citri biological characteristics and associated ecological environment characteristics through the ecological characteristic library to form an initial ecological characteristic set; fusing the real-time monitoring data with the initial ecological feature set to generate a dynamic ecological feature set; calculating the priority score of each prevention and control strategy according to the spatial and temporal distribution density, migration trend intensity, natural enemy inhibition coefficient, host plant resistance index and environmental stress factor of each feature in the dynamic set; generating a strategy sorting table in a descending order according to the scores, sequentially selecting to-be-executed strategies, and judging whether the to-be-executed strategies complete the target population density reduction amount or not in a preset period. According to the method, real-time data and ecological characteristics are combined, the strategy is dynamically adjusted, and the prevention and control accuracy and effectiveness are improved.

Transgenic plants with blue flower color, or their inbred or outbred progeny, or their propagules, partial plant bodies, tissues or cells, are provided. A buckwheat-derived C-glucosyltransferase (CGT) gene or its homolog is transferred into a host plant to cause delphinidin-type anthocyanins and flavone mono-C-glycosides to be copresent in the plant cells.

The application discloses a LAMP primer group, a kit and a detection method for detecting Pinus armandii rust fungus, and belongs to the technical field of genetic engineering. The application takes the specific nucleotide sequence of the large subunit ribosomal RNA of the Pinus armandii rust fungus as a target gene to design and screen a primer group which is specific and high in sensitivity, and can be used for LAMP detection of the Pinus armandii rust fungus. The LAMP primer group provided by the application has the advantages of high universality, high specificity, high sensitivity and good accuracy, and the detection limit can reach 4.5x10 ‑4 ng / μL, which is 100 times higher than the sensitivity of the LAMP detection of different species in the same genus in the prior art, the detection method can rapidly detect the Pinus armandii rust fungus from spore piles and host plant materials, and is suitable for the detection of Pinus armandii seedling materials in forest fields and customs quarantine ports.

The invention discloses a diaphorina citri effector protein DcPDI1 and an application thereof. The amino acid sequence of the diaphorina citri effector protein DcPDI1 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the diaphorina citri effector protein DcPDI1 is as shown in SEQ ID NO. 2. After the diaphorina citri DcPDI1 is knocked out, the egg laying amount and the developmental duration of the diaphorina citri are remarkably reduced, and it is indicated that the DcPDI1 plays an important role in growth and development of the diaphorina citri. The diaphorina citri effect protein DcPDI1 can be a new molecular target spot, plays a key role in activating defensive response of host plants and helping diaphorina citri to eat, and provides a new insight for a molecular mechanism of interaction between diaphorina citri and plants.

The present invention relates to an efficient method for artificially synthesizing Lactarius deliciosus mycorrhiza, which comprises the following steps: (1) Take the mycelium with a size of 1-3 mm at the connection of the stipe and cap of fresh Lactarius deliciosus fruit bodies, place it in a PDA medium, and conduct tissue culture at 20-25 °C for 20-30 days. Then, take the mycelium block and inoculate it onto the PDA medium, and continue to culture at 20-25 °C for 25-40 days. Subsequently, take the mycelium block again and inoculate it into a PDB medium for culturing for 30-50 days to obtain Lactarius deliciosus liquid spawn; (2) Transplant pine seedlings at a density of 6-15 seedlings per basket into a culture basket filled with mycorrhizal substrate, add the Lactarius deliciosus liquid spawn prepared in step (1) to the substrate 3-5 cm deep near the root of each seedling, and mycorrhiza can be formed after culturing for 10-14 days. The present invention scientifically screens the substrate and method for Lactarius deliciosus. By using the optimized medium formula and culture method, the mycelium of Lactarius deliciosus grows faster and the biomass of the obtained Lactarius deliciosus mycelium is larger; inoculating the plant roots with liquid spawn greatly improves the inoculation efficiency; multiple inoculated host plants are cultured in the same culture device, which greatly shortens the mycorrhiza formation time after inoculation and effectively improves the mycorrhiza synthesis rate; reducing the amount of peat soil in the mycorrhizal substrate reduces the proportion of mycorrhiza formed by miscellaneous bacteria and pine roots.

The invention discloses a solid-state fermentation process of plant endophytic actinomycetes OsiLf-2, a spore storage method and application. The solid-state fermentation process comprises the following steps: preparing a solid-state fermentation culture medium, inoculating a seed solution for solid-state fermentation, and collecting spores. The solid-state fermentation process disclosed by the invention can produce spores on a large scale, shortens the fermentation period, and has the advantages of simple process, convenience in operation, short fermentation period, low bacterial contamination rate, low raw material cost, high sporulation quantity and the like; the obtained spores can be freeze-dried under the protection of a skimmed milk powder solution to form spore powder, so that the freeze-drying survival rate and the long-term storage stability of the spores can be improved. The spore provided by the invention can be used for promoting crop growth, is more environment-friendly to ecology especially when being used for preparing a microbial fertilizer, does not influence the agricultural ecological environment and human body health, can promote the growth and development of host plants, and is high in use value and good in application prospect.

The invention discloses a protein capable of inducing a plant immune activation function and application of the protein in disease control, the gene sequence of the protein capable of inducing the plant immune activation function is shown as SEQ ID NO: 1, and the amino acid sequence of the protein is shown as SEQ ID NO: 2. According to the invention, a novel plant immune activator protein CgSsp16 secreted by colletotrichum gloeosporioides is identified, can be identified by plants, stimulates a plant immune response mechanism, triggers broad-spectrum resistance of host plants to various pathogens and improves disease resistance of various plants; the compound is expected to be developed into a novel biopesticide to be applied to green and sustainable production of agriculture.

The present invention discloses the development and application of a genomic editing tool for phytopathogenic bacteria. The present invention provides a DNA molecule I, which has element A, element B, and element C; element A is an expression cassette for expressing the ligD gene and the Ku gene; element B is an expression cassette for expressing the Cas12a gene; the element C has an insertion site and a DNA segment for encoding the crRNA backbone; the insertion site is for inserting a DNA segment for encoding the specific binding region of the crRNA; the specific binding region of the crRNA specifically binds to the target sequence. The present invention constructs a set of genomic editing tools suitable for phytopathogenic bacteria by fusing the ligD / Ku protein with the CRISPR / FnCas12a system. This method adds a new powerful tool to the genetic analysis toolbox of phytopathogenic bacteria and has great value for studying the molecular interaction mechanism between host plants and pathogenic bacteria.

Disclosed are endophyte inoculant compositions, methods for making such compositions, methods for using such compositions, and physiologically altered plants treated with such compositions. The endophyte inoculant compositions may include one or more of the endophytic species WW5, WW6, WW7, and PTD1, and when applied to a non-native host plant, promote plant mineral nutrient acquisition and uptake, vigor, health, growth, and yield.

The application provides cassava MeFER4 Gene and application thereof in improving sensitivity of plants to stress. By constructing overexpression vector containing MeFER4 Gene and transforming into host plant tissues such as Arabidopsis and cassava through infection method, plants sensitive to drought, abscisic acid, oxidation, salt and osmotic stress and other stress are obtained. Research shows that, MeFER4 It is a negative regulatory factor of plant stress response and plays a negative regulatory role in plant drought resistance and the like, and the transgenic plant is more sensitive to stress. The application is beneficial to elucidating the role of MeFER4 Gene in stress response from the molecular mechanism, and has important significance for directional genetic improvement of plant drought resistance and plant variety selection.

The present application relates to the technical field of identifying Cynomorium songaricum, in particular to a method for rapidly identifying Cynomorium songaricum with different processing methods and host plants based on electronic nose technology, comprising the following steps: sample processing; 1.0 g of sample is loaded into a 10 mL silica gel covered syringe bottle, and after 25 min of water bath balancing at 60 DEG C, the sample is detected by an electronic nose; and statistical analysis. The present application uses electronic nose technology to detect and analyze Cynomorium songaricum with different processing methods and from different host plants, to understand the influence of different host plants and processing methods on the flavor of Cynomorium songaricum, and to provide a strong theoretical basis for the rapid quality identification and characteristic flavor evaluation of Cynomorium songaricum. In data analysis, the Gaussian Naive Bayes (GNB) classification model statistical method is used to qualitatively identify the characteristic odor of Cynomorium songaricum through the retention value of the electronic nose, and a discrimination model is constructed to lay a solid theoretical foundation for future qualitative prediction.

The application discloses application of alpha-pinene and citral in combination in luring Spodoptera frugiperda, and belongs to the technical field of biological control. The application determines habitat selection and oviposition selection preference of Spodoptera frugiperda on different host plants, collects volatiles of nine kinds of host plants preferred by Spodoptera frugiperda by using a dynamic headspace method, and successfully screens out alpha-pinene and citral combination with good luring effect on female and male adult Spodoptera frugiperda by means of GC-MS technology, indoor behavior selection experiment and semi-field verification. Alpha-pinene and citral both belong to natural plant volatiles, and have the characteristics of easy acquisition, low cost, environmental friendliness and the like, and can serve as background materials or combined components of efficient luring agents of Spodoptera frugiperda. The application can effectively promote the construction and improvement of green control technology of Spodoptera frugiperda, and provides a new and effective tool for Spodoptera frugiperda control work.

A lysogenic phage community, its extraction method and application. The tangential flow technology is used to proportionally isolate bacteria and phage communities from in-situ soil, and single-bacteria communities and single-phage communities are obtained through enrichment and concentration. At the same time, mitomycin C is added to the single-bacteria community for lysogenic induction, and then the tangential flow system is used again for filtration and enrichment to obtain a high-throughput lysogenic phage community. Finally, we obtain single-bacteria communities, bacteria-phage mixed communities, and bacteria-lysogenic phage mixed communities. The phage transplantation technology can promote the participation of indigenous microorganisms in the process of promoting plant growth and development. The transplanted lysogenic phages can participate in the synthesis and metabolism of host plant hormones by optimizing the microbial network structure and injecting auxiliary metabolic genes. This method provides a long-term and environmentally friendly microbial synergistic growth promotion technology for the development of green agriculture in China.

The invention relates to the technical field of biological purification, and particularly discloses a separation and purification method of citral type cinnamomum bodinieri leaf and stem endophytic fungi, which is used for solving the problem that subsequent purification is difficult due to existing disinfection. Comprising the following steps: step 1, preparing absolute ethyl alcohol with a volume ratio of 75% by using distilled water, and preparing sodium hypochlorite with a volume ratio of 5.0-10.0% for later use; 2, stem and leaf samples of the sample plants are collected and immediately brought back to a laboratory and washed with running water till no impurity exists on the surfaces of stem and leaves, and villi on the surfaces of the leaves are washed away; 3, the stems and the leaves are sequentially soaked with sterile water for 2 min, soaked with 75% ethyl alcohol for 1 min, washed with sterile water for 3 times, treated with sodium hypochlorite for 5-15 min and washed with sterile water for 3 times; the citral type cinnamomum bodinieri is used as a test material, the optimal surface disinfection method for citral type cinnamomum bodinieri leaf and stem tissues is explored, the endophytic fungi are separated, purified and identified, the endophytic fungi with chemical components similar to those of host plants are screened, and reference is provided for development, utilization and deep research of the citral type cinnamomum bodinieri endophytic fungi.

The invention belongs to the technical field of plant resistance breeding, and particularly discloses a parasitic plant resistance evaluation method. The method comprises the following steps: establishing an in-vitro co-culture system of host plants and parasitic plants; respectively counting the germination rate, nodule number, stem emergence rate and death rate of parasitic plants in the host plant resistance variety and the host plant susceptible variety at a plurality of time points; preliminarily judging the type of a resistance mechanism according to the germination rate difference; preparing interaction tissue slices of the host plant and the parasitic plant according to the type of the resistance mechanism; and evaluating the parasitic process in the interaction tissue slice to obtain stage-specific defense response characteristics, and outputting a resistance evaluation report. The method can systematically distinguish whether a parasitic plant resistance mechanism belongs to early signal obstacle or parasitic stage defense, realizes rapid and accurate positioning of a key stage of resistance action in a laboratory, and provides an accurate theoretical basis and an efficient screening tool for resistance breeding.

The present application belongs to the technical field of artificial propagation of natural enemy insects and biological control of agricultural pests, and discloses a method for mixed feeding of two kinds of aphid parasitic wasps, which mainly has the following technical features: the steps include breeding of host plants, inoculation and propagation of aphids, obtaining and inoculation of two kinds of aphid parasitic wasp eggs, collection of parasitic wasps, removal of impurities, and separation of the two kinds of parasitic wasps. The method for mixed feeding of two kinds of aphid parasitic wasps provided by the present application inoculates two kinds of parasitic natural enemies, i.e. Aphidius gifuensis and Encarsia spp., at different time periods, improves the parasitic efficiency of single wasps, avoids interspecific competition between adult wasps, collects the two kinds of parasitic wasps at the same time, and saves manpower and material resources.

A method for controlling thrips using Stratiolaelaps scimitus during float-tray-based tobacco seedling cultivation, including the following steps: 1) arranging a seedbed in a wind-sheltered sunny area with upwind positioning, and removing host plants of the thrips around the seedbed; 2) sowing 2 tobacco seeds per cell in float trays for the float-tray-based tobacco seedling cultivation, arranging 32 float trays per nursery greenhouse, placing the float trays in a nutrient solution tank, adding a formulated fertilizer solution for the float-tray-based tobacco seedling cultivation into the nutrient solution tank, arranging a plurality of nursery greenhouses in close proximity to form a nursery greenhouse cluster, erecting a plastic film vertically around the nursery greenhouse cluster, and arranging an insect-proof net around the nursery greenhouse cluster; and 3) after a rate of seedling emergence reaches over 90%, thinning seedlings.

The invention belongs to the technical field of ecological prevention and control of agricultural pests, and relates to a peach fruit moth sex pheromone communication interference composition derived from host plants. The interferent is selected from at least one of trans-2-heptanal, trans-2-hexenyl acetate, leaf acetate and octanoic acid. The interferent can be used for predicting, forecasting, preventing and controlling peach fruit moths. The communication interferent provided by the invention can influence chemical communication between female and male imagoes of the peach fruit moth. The method can be combined with sex pheromones to be used for predicting, forecasting, preventing and controlling the peach fruit moths, and a new combination is provided for ecological prevention and control of the peach fruit moths.

The invention discloses a solid-state fermentation process of plant endophytic actinomycetes CoT10, a spore storage method and application. The solid-state fermentation process comprises the following steps: preparing a solid-state fermentation culture medium, inoculating a seed solution for solid-state fermentation, and collecting spores. The process disclosed by the invention can produce spores on a large scale, can shorten the fermentation period, and has the advantages of simple process, convenience in operation, short fermentation period, low bacterial contamination rate, low raw material cost, high sporulation quantity and the like; meanwhile, the obtained spores can be freeze-dried to form spore powder under the protection action of trehalose, cane sugar and a sodium glutamate solution, so that the survival rate and the stability of the spores in long-term storage can be improved. The spore provided by the invention can be used for relieving heavy metal stress of plants and promoting plant growth, is more environment-friendly to ecology when being used for preparing a microbial fertilizer, does not influence an agricultural ecological environment and human body health, can promote growth and development of host plants, and is high in use value and good in application prospect.

The artificial efficient breeding method comprises the following steps that S1, host plants are planted in a spawning room, and the host plants are gramineae plants or asparagus plants which are easy to plant and have developed root systems; s2, collecting mature golden cicada nymphs which come out of the ground and are not subjected to eclosion, putting the collected mature golden cicada nymphs into an artificial climate box for artificial eclosion, and after eclosion and bee emergence, selecting parent adults; s3, putting the adults into a mating cage of an oviposition room according to a male-female ratio for mating, wherein an adult luring device consisting of a male cicada chirp generating device and a sex pheromone trap is arranged in the oviposition room. By arranging the adult luring device composed of the male cicada chirp generating device and the sex pheromone trap, the mating rate of the male cicada and the female cicada is synergistically increased, and technical support is provided for subsequently increasing the breeding rate of a cicada provenance; by means of the breeding method, efficient artificial breeding of the golden cicada seed source is achieved, and a seed source resource is provided for large-scale breeding of the golden cicada.