681 results about "Host plants" patented technology

The invention discloses a special promoter separated from millet, expressing carrier with nucleic acid sequence of SEQ ID No. 1 host with the expressing carrier and appliance of the promoter, which is characterized by the following: utilizing Tail-PCR (colored body step moving method); getting the special promoter from gene group DNA; possessing nucleic acid sequence of SEQ ID No. 1; ;linking downstream of the promoter to non-homologous or homologous gene; constructing plant expressing carrier; transferring host plant; driving the downstream gene to high effective and special express goal protein in the seed; realizing genetic modification of plant; or using as effective tool for studying plant and biological reactor.

Methods are provided for the genetic control of pathogen infestation in host organisms such as plants, vertebrate animals and fungi. Such methods utilize the host as a delivery system for the delivery of genetic agents, preferably in the form of RNA molecules, to a pathogen, which agents cause directly or indirectly an impairment in the ability of the pathogen to maintain itself, grow or otherwise infest a host plant, vertebrate animal or fungus. Also provided are DNA constructs and novel nematode nucleotide sequences for use in same, that facilitate pathogen resistance when expressed in a genetically-modified host. Such constructs direct the expression of RNA molecules substantially homologous and/or complementary to an RNA molecule encoded by a nucleotide sequence within the genome of a pathogen and/or of the cells of a host to effect down-regulation of the nucleotide sequence. Particular hosts contemplated are plants, such as pineapple plants, and particular pathogens are nematodes.

By this invention, modification of the fatty acid composition of a plant seed may be achieved as a result of the activity of a DNA sequence foreign to the plant species to be modified. In particular, it has been found that a plant oil having a modified fatty acid composition can be obtained upon the expression of genes derived from plants of different species than the host plant, upon the expression of genes derived from bacteria, and from the transcription of anti-sense sequences which are complementary to endogenous genes of the plant host cell. In a preferred embodiment, transcription of the fatty acid modifying foreign DNA sequence is restricted to the developing seed tissues.

Cytochrome b5 (Cb5) is a haem-binding protein located in the endoplasmic reticulum (ER) and the outer mitochondrial membranes of higher eukaryotes. In higher plants, animals, and fungi, the ER resident Cb5 has been shown to play a role in desaturation of acyl CoA fatty acids. Higher plants Cb5 isoforms from plants such as soybean or Arabidopsis are capable of modulating omega-3 desaturation. Co-expression of certain Cb5 isoforms with FAD3 in a host plant results in increased production of seed oil content as well as altered ratio between different fatty acids. It is also disclosed here that overexpression of Yarrowia ACL enzymes in the plastids of a host plant helps boost the synthesis of acetyl CoA, which in turn, may lead to increased synthesis of fatty acids and enhanced oil accumulation in the seeds.

Novel plant promoters isolated from cotton fiber tissues are provided. The plant promoters are located upstream cotton plant genes KC03, KC18, KC22, and Gh3, all of which are expressed in cotton fibers, and contain (a) DNA having the nucleotide sequence of SEQ ID NO:1, 6, 11, or 16. Also provided are plant expression vectors prepared by introducing the plant promoters into appropriate vectors; transformed plant cells prepared by introducing the plant expression vectors into host plant cells; transformed plants regenerated from the transformed plant cells; transformed plant seeds obtained from the transformed plants; and processes for producing transformed plants from the plant seeds.

The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.

The invention discloses a method for propagating Phytoseiulus Persimilis, which comprises the steps: planting and cultivation of host plant, inoculation and feeding of Tetranychus cinnabarinus, inoculation and feeding of Phytoseiulus Persimilis as well as collection, package and refrigeration of Phytoseiulus Persimilis. The method takes mass propagation of Phytoseiulus Persimilis as the purpose, provides technical support for feeding Phytoseiulus Persimilis on scale, and provides special predacity natural enemy for Tetranychus pest mites on greenhouse crops for biological control. The invention is a production method in which living hosts (firstly host plant and host Tetranychidae are cultivated) are adopted for feeding Phytoseiulus Persimilis, and makes a series of breakthroughs on key techniques for artificial mass propagation of Phytoseiulus Persimilis. The method of the invention can lead the production cost of Phytoseiulus Persimilis to be stabilized, the production period to be 25 days, and the production scale to reach 10,000/m<2>.

The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.

By this invention, novel nucleic acid sequences are provided, wherein said nucleic acid sequence is a genomic sequence of a plant desaturase encoding sequence. Also provided in the present invention are the promoter and intron sequences of the desaturase genomic sequences. Furthermore, recombinant DNA constructs employing the polynucleotide sequences are provided. The instant invention also provides methods for the modification of fatty acid compositions in host plant cells.

A transactivation system and method for producing a foreign polypeptide of interest in cells of a host plant is disclosed. The transactivation system has two components. It has genetically transformed cells of the host plant having integrated in their nuclear genome, an inactive or silenced foreign nucleic acid sequence, which is capable of encoding, upon its activation, the foreign polypeptide of interest; and a recombinant RNA viral vector capable of infecting the cells of the host plant and encoding therein a factor for activating or facilitating the expression of inactive or silenced foreign nucleic acid sequence.

The invention relates to an overwintering preserving breeding method for an aphidius gifuensis and belongs to the technical field of biological control. The overwintering preserving breeding method for the aphidius gifuensis solves the problem that in the prior art, overwintering preservation is performed on the aphidius gifuensis difficultly, and the heavy demand for the aphidius gifuensis in a tobacco planting season in a coming year is difficult to meet. The overwintering preserving breeding method comprises a first step of greenhouse aphidius gifuensis selection which comprises cultivation of host plants, collecting of an aphidius gifuensis source, aphidius gifuensis inoculation in a greenhouse, and purification and rejuvenation; a second step of winter greenhouse aphidius gifuensis preservation which comprises cultivation of aphidius gifuensis preserving plants, aphidius gifuensis inoculation in the greenhouse, and purification and rejuvenation; and a third step of expanding propagation in the greenhouse. The aphidius gifuensis is successfully accumulated and preserved during the time from finish of massive reproduction of the aphidius gifuensis in August to April in a coming year and before massive reproduction of the aphidius gifuensis, and the aphidius gifuensises with enough quantity and good quality are provided for massive reproduction of the aphidius gifuensis in a next year.

Disclosed is a large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae. The yellow-green myzuspersicae with large bodies serve as host aphids of aphidiusgifuensis, and parent aphid disease-resistant leafy cruciferous green vegetables, radishes or sweet blue flowers serve as host plants of the yellow-green myzuspersicae, so that the mummified aphids and the adult bees of the aphidiusgifuensis are artificially bred on a large scale. The method solves the problems that the bodies of the host aphids are small, the mummified aphids are difficultly collected, the eclosion rate of the aphidiusgifuensis is low, successful egg laying amount is small and industrialized production is not easily realized when the aphidiusgifuensis is artificially bred. Collecting and breeding technology of the host aphids and species of the aphidiusgifuensis is provided in the artificially breeding process, the technology of host plant cultivation, inoculation breeding of the aphids and breeding of the mummified aphids and the adult bees of the aphidiusgifuensis is provided, the method has the advantages of short period, high efficiency, high breeding speed and low cost, the mummified aphids of the aphidiusgifuensis can be largely bred, the mummified aphids and the adult bees of the aphidiusgifuensis are produced on a large scale, and the myzuspersicae, English grain aphids and cotton aphids can be effectively prevented in the technical field of biological prevention.

A protoporphyrinogen oxidase tolerant to photobleaching herbicide and derivatives thereof, comprising a polypeptide having the amino acid sequence represented by SEQ ID No. 2 or mutated peptides derived therefrom by deletion, addition, substitution, etc. of one or more amino acids in the above amino acid sequence and having an activity substantially equivalent to that of the protoporphyrinogen oxidase. The acquisition of the novel protoporphyrinogen oxidase, which is highly tolerant to photobleaching herbicide and originates in plant, make it possible to construct plants highly tolerant to photobleaching herbicide via the expression of this enzyme in host plants.

The invention discloses a method for biologically restoring organic phosphorus pesticide polluted soil. An arbuscular mycorrhizal fungi and plant symbiotic system is used for accelerating the recovery of the organic phosphorus pesticide polluted soil. The method comprises the following steps of: (1) preparing an arbuscular mycorrhizal fungi agent; (2) inoculating the fungi agent to the soil to be restored, and planting quick growth plants, or transplanting the plants after mycorrhizal seedling culture; and (3) managing the plants to grow 2 to 3 months. The arbuscular mycorrhizal fungi agent comprises Glomus caledonium 90036, Acaulospora mellea ZZ, Glomus mosseae BEG167 and Glomus intraradices BEG 141). The host plant is selected from quick growth plants such as Sudan grass, alfalfa, corn, broomcorn and the like. The method can reduce the pesticide residue in agricultural products, reduce environment transfer to water, atmosphere and the like and reduce harm to human health, and is simple, convenient and environment-friendly.

A method for inoculating the tubeflower cistanche onto Chinese tamarisk includes such steps as transplanting Chinese tamarisk in the summer or autumn, digging the inoculating groove to expose the fibrous roots of Chinese tanarisk in the next spring, using clean water to flush the exposed fibrous roots, attaching the chosen seeds of tubeflower cistanche to them, filling soil in the groove, and ramming the soil.

The invention relates to the technical field of automobile trading, in particular to a method, a device, a device and a storage medium for sharing automobile data based on a block chain. The methods include: the host plant node obtains a unique identification corresponding to the automobile information and the automobile information, generating an automobile detail block, and uploading the block in the block chain system to form a new block chain; the host plant node broadcasts the uploaded automobile detail block to other nodes; after receiving the broadcasting, the other nodes record the unique identification, the automobile information and the hash value; The dealer node obtains maintenance information, generates maintenance blocks, and uploads and broadcasts them. The invention introduces a block chain system and constructs an information sharing system for automobiles from production to sale by virtue of the decentralization characteristic of the block chain, realizes the purposeof multi-participation of each link and joint maintenance of automobile data on the block chain, and provides reliable automobile data for the estimation of used automobiles.

The invention provides a method for propagating Aphidius gifuensis by pea aphids. The method provided by the invention utilizes two facilities including a seedling culture big shed and a field small shed and takes broad beans and peas as host plants for propagating aphids; the method takes the pea aphids as host aphids for propagating Aphidius gifuensis and then the propagated Aphidius gifuensis are released to a large-scale tobacco field to kill the aphids on tobacco leaves. The method selects the broad bean seeds and the pea seeds and then tender green bean plants are cultivated in a big greenhouse or the small shed built in the fields; the pea aphids are inoculated on the bean plants and then the pea aphids are propagated; then the Aphidius gifuensis is inoculated on the tender bean plants which is propagated with a lot of pea aphids and then the Aphidius gifuensis is propagated. Then a lot of propagated Aphidius gifuensis are released into the large-scale tobacco field to kill theaphids on tobacco leaves. Finally, the aim of biologically preventing and treating aphids is realized and the pesticide pollution caused by preventing the aphids is avoided.

A soil improver and method for making the same. The soil improver, in one example, comprises a nitrogen source, a surfactant, a multi-mineral, and a microbial blend. The microbial blend comprises at least one bacterium, at least one fungus, and at least one mycorrhiza. The mycorrhizae mine water and nutrients for plant roots in exchange for food. Further the mycorrhizae greatly expands the effective root zone of the host plant. The soil improver is made by mixing the dry ingredients. Thereafter the microbial blend is mixed with the dry ingredients.

The invention relates to a method for artificial culture and propagation of plant root-knot nematodes and for species preservation. The propagation and preservation of the root-knot nematodes in a lab depend on host plants all the time; the invention places a relatively small amount of root-knot nematode ovums or 2nd instar larvas in a particular dual culture medium rather than host roots and performs a upside-down culture under conditions of constant temperature and hermetic closure, the root-knot nematodes ingest, grow and propagate inside the culture medium and a large amount of nematomorphas can be obtained in a short term; additionally, a culture plate is timely transferred to be located under cold conditions, and dish change and subculture are implemented once within a half year so that the propagation and preservation processes of the root-knot nematodes are carried out without the host plants. The inventive advantages lie in: saving the culture program of the root-knot nematodes to host the plants without losing the infectivity of the root-knot nematodes on sensitive host plants; the employed materials have low costs and the experiment method is easy in operation; the invention provides a simple, convenient and fast propagation method for the species preservation of the root-knot nematodes, in particular for a large number of nematomorphas as required in the related researches of the root-knot nematodes.

The invention discloses a scale breeding method for encarsia formosa. The scale breeding method is finished in four greenhouses, namely a host plant cultivating room, a worm species room, an encarsia formosa species room and a breeding room, by steps. The scale breeding method comprises a host plant cultivating and managing step, a whitefly species managing step, a whitefly inoculating step, a whitefly nymph breeding step, an encarsia formosa species managing step, an encarsia formosa inoculating step, an encarsia formosa pupa breeding step, a pupa collecting step, a worm card or encarsia formosa box manufacturing and storing step and a whitefly controlling step. The scale breeding method has the advantages that process steps are continuously circulated and the encarsia formosa can be annually produced and bred. The scale breeding method is suitable for industrial scale production and reproduction of the encarsia formosa and is short in reproduction period, high in efficiency, low in energy consumption and low in cost. The obtained adult encarsia formosa, encarsia formosa pupa card or encarsia formosa box products are good in quality. The problem of insufficient supply for control over bemisia tabaci and trialeurodes vaporariorum in agriculture and forestry is solved.

The invention discloses a method for breeding larva of lepidoptera insects indoor. The breeding box using in the method has no bottom, and the lower part of the inner wall of the breeding box is fixedly connected with at least two support blocks. A leaking frame is arranged on the support blocks, at least one fixing device of a cutting transplanting pipe is arranged on the leaking frame, and at least one cutting transplanting pipe is arranged in the fixing device of the cutting transplanting pipe. The lateral wall of the breeding box or the box cover is provided with at least one ventilating window. The leaking frame comprises a cross rod and a grid. The branches of the host plant are inserted in the cutting transplanting pipe, the leaves of the plant branches cannot touch the inner wall of the breeding box, and clean water or plant culture medium is put in the cutting transplanting pipe. The invention solves the technical problems that the larva difficultly eats food caused by disorderly climbing, and the larva groups are easy to crossly infect by pathogen caused by that excrement of the larva pollutes the leaves. The invention can adjust the temperature and the humidity in the breeding box, has long fresh keeping period, can conveniently change the branches and clean the box body, can easily disinfect, and has a simple and smart structure, low cost and little labour input, so it is suitable for breeding the larva of large insects indoor.

The present invention relates to Glomus mosseae 93-6 as one kind of efficient drought-resisting and high phosphate-tolerant nutrients bush mycorrhizal fungus and its production process. The Glomus mosseae preparation is prepared with kaoliang as host plant, mixture of zeolite and sand as culture medium. The dried preparation contains 40-65 spores in each ml. Glomus mosseae may be grown in various kinds of plants to improve nutrients absorption, raise drought resistance and promote growth of plant. Test shows that the present invention has excellent seedling strengthening and yield increasing effect on straw berry, rice, water melon and other crops.

The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell, wherein the T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase and optionally a DNA of interest linked to the viral replicon. The DNA of interest may also contain a non-identical target site for the recombinase. An expression cassette for the site-specific recombinase is present on the T-DNA or the plant genome, or is transiently introduced into the plant cell. Expression of the site-specific recombinase in the plant cell results in excision of the viral replicon and the associated DNA of interest. The viral replicon and DNA of interest are then replicated to high copy number in the host plant cell. The compositions of the invention comprise nucleic acids, such as T-DNAs containing a viral DNA flanked by directly repeated target sites for a site-specific recombinase. The nucleic acids of the invention may additionally contain expression cassettes encoding the cognate site-specific recombinase for the target sites flanking the viral genome. The compositions of the invention further comprise Agrobacterium containing the nucleic acids of the invention. The compositions and methods of the invention have use in increasing the efficiency of agroinfection, providing high copy numbers of a DNA of interest for transient expression or for integration into a plant chromosome, and in simplifying the construction and stable maintenance of vectors for agroinfection and transformation.

The invention discloses a method for artificial feeding of tobacco whiteflies, which belongs to the technical field of insect feeding. The method provides a feed formula for the artificial feeding of the tobacco whiteflies and a method for feeding by applying the feed, and the method comprises the steps of the acquisition and disinfection of tobacco whitefly eggs, the preparation of an artificial all-nutrition feed liquid, the preparation of a feeding container, the feeding and so on. The method has the advantages that the method realizes the aim of the indoor artificial feeding of the tobacco whiteflies all the year around, can obtain various instar insects with relatively consistent biological type, instar stage, state and so on, overcome the defect that easy influence by different host plants, biological types, growth states, instar stages and so on, caused by acquiring insect sources from natural birthplaces leads to the defect that the difference exists in experimental results, and ensure the standardization of test insects for experiments. The method can be used for experimental research on biological characteristics and a high-efficiency prevention and control technology of the tobacco whiteflies.