210 results about "Host plants" patented technology

The invention relates to the technical field of agricultural micro-ecological application, and discloses a method for improving the growth and drought resistance of oats by using arbuscular mycorrhizal fungi, the arbuscular mycorrhizal fungi having a close symbiotic relationship with the oats are selectively utilized, and the symbiotic relationship between the arbuscular mycorrhizal fungi and the oats is established in a condition control mode. Oat growth is promoted, and drought adaptability is improved. The AM fungi used in the method are selected from rhizosporidium intraradices or glomus mosseae, corn or trifolium repens is taken as a host, the two AM fungi are propagated in a propagation medium respectively, then two arbuscular mycorrhizal fungi inoculants and oat are co-cultured in a symbiotic culture medium respectively, and the two arbuscular mycorrhizal fungi inoculants and oat are co-cultured in a symbiotic culture medium respectively. An effective way for improving beneficial symbiosis of AM fungi and hosts under adverse conditions is provided, the infection symbiosis relation of the AM fungi to host plants is improved manually, and the yield of dry land oats is increased.

The invention provides a picea flower monochamus alternatus attractant and a use method thereof.The attractant comprises plant source pheromone and insect pheromone, the chemical components are mixed according to a certain dosage and proportion and then dropwise added to slow-release carriers such as a slow-release bottle, a slow-release bag and a capillary tube, and a picea flower monochamus alternatus lure is prepared; the insecticidal composition can be used for trapping a large number of black longhorn beetles of picea flowers and has the characteristics of remarkable trapping effect and longer lasting period. The attractant is simple to prepare, good in attraction effect and not prone to generating resistance, the formula is a combination of host plant volatile matter and insect pheromone, the attractant is harmless to people and livestock and free of pollution to the environment, a practical pollution-free monitoring and prevention technical means is provided for large-scale trapping and killing of the black longhorn beetles in forests, forest resources are protected, and the economic benefits are increased. The ecological balance is maintained, the application value is very good, and the sustainable development of forestry is promoted.

The invention discloses a dsRNA nano nucleic acid insecticide for preventing and treating piercing-sucking pests, the dsRNA nano nucleic acid insecticide is composed of dsRNA of a specific targeting piercing-sucking pest Chs gene and a carrier, the piercing-sucking pests are Panonychus citri, Diaphorina citri or Aphis gossypii, the nucleotide sequences of the Chs gene are respectively shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3, and the carrier is MgAl or MgFe layered double-metal hydroxide nanoparticles. The dsRNA nano nucleic acid insecticide is applied to host plants of the piercing-sucking pests, after the plants induce the piercing-sucking pests to eat the dsRNA nano nucleic acid insecticide, RNA synthesis and silent Chs genes are interfered in vivo, death is promoted, and the dsRNA nano nucleic acid insecticide has good application prospects in treatment of the piercing-sucking pests.

The invention discloses an arbuscular mycorrhizal fungus inoculant for improving the yield of medicago truncatula in low-phosphorus soil as well as a preparation method and application of the arbuscular mycorrhizal fungus inoculant. The arbuscular mycorrhizal fungus inoculant comprises a culture medium, spores and ectogenic hyphae of arbuscular mycorrhizal fungi mixed in the culture medium, and a host plant root system infected by the arbuscular mycorrhizal fungi, the culture medium is river sand, and the arbuscular mycorrhizal fungi comprise glomus terrestris, Aureobasidium microconcave, glomus intraradices, glomus mosseae, glomus twistula and glomus macrosporium; the root system of the host plant infected by the arbuscular mycorrhizal fungi is the root system of the corn and alfalfa infected by the arbuscular mycorrhizal fungi. According to the method, different arbuscular mycorrhizal fungi strains such as glomus terrestris, aureoconcave aureoconcave, glomus mosseae, glomus intraradices, glomus twistula and pearl macrosporium are mixed according to a certain proportion, so that the phosphorus obtaining capacity of the host plant in a low-phosphorus soil environment can be effectively enhanced, and the yield of the host plant is further remarkably increased; meanwhile, the arbuscular mycorrhizal fungus inoculant can partially or completely replace phosphate fertilizer according to the soil phosphorus level of a specific pasture planting environment, so that related environmental pollution is reduced, and sustainable agricultural development of environmental ecology is facilitated.

The invention provides dsRNA (double-stranded ribonucleic acid) targeting a BcMucin gene and application of the dsRNA in preparation of a preparation for preventing and treating gray mold. The dsRNA is obtained by taking a coding sequence of a target gene BcMucin as a template through manual design and in-vitro synthesis. The dsRNA with the BcMucin as the target can be used for effectively inhibiting the occurrence of the botrytis cinerea. Experiments prove that after the deletion of the target gene BcMucin provided by the invention, botrytis cinerea hyphae grow slowly, the pathogenicity to tomatoes is completely lost, and the target gene BcMucin is a key gene for botrytis cinerea growth and pathogenicity regulation. The dsRNA provided by the invention can effectively interfere the expression of a target gene BcMucin in botrytis cinerea so as to inhibit the growth and development of the botrytis cinerea and the infection of the botrytis cinerea on host plants and reduce the biomass of the botrytis cinerea on scabs. The dsRNA provided by the invention can be used for RNA pesticide development based on RNAi, is applied to effective prevention and treatment of gray mold, and has a good market application prospect.

The invention discloses an endophytic fungus and application thereof, the endophytic fungus strain is 0522-8-2, the classification name is Aspergillus tubingensis, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is January 3, 2024, and the preservation number is CGMCC No.41026; the salt-tolerant plant root endophytic fungi 0522-8-2 can obviously improve the survival rate of forest tree cuttings under salt stress and relieve the adverse effects of salt stress on the growth traits, physiology and photosynthesis of host roots; phosphorus is dissolved and alkali is controlled by secreting organic acid, so that absorption of a host plant to insoluble phosphorus is improved, and meanwhile, the adverse effect of high pH of saline-alkali soil on plant growth is relieved; therefore, the method is suitable for popularization and application.

The invention discloses a method for inoculating cistanche with atriplex canescens in the technical field of plant cultivation. The method comprises the following steps of host plant selection and cultivation, cistanche seed treatment, inoculation, cultivation management, harvesting and processing. The method has the advantages that the parasitic success rate is increased and can reach 80% or above generally; the atriplex canescens grows well, sufficient nutrients are provided for the cistanche deserticola, and the yield of the cistanche deserticola is remarkably increased and is increased by 30%-50% compared with a traditional method. And 3, the ecological benefit is remarkable: the planting of the atriplex canescens is beneficial to improving the soil structure and the ecological environment, and meanwhile, the growth of the cistanche deserticola also provides protection for the atriplex canescens, so that the mutualism of the atriplex canescens and the cistanche deserticola is realized, the vegetation coverage rate of a desertification region is enhanced, and the soil structure and the ecological environment are improved. And 4, the economic benefits are considerable, large-scale planting can be realized, a new way is provided for increasing income of farmers, and the method has good market prospects and economic benefits.

The invention relates to the technical field of cynomorium songaricum identification, in particular to a method for rapidly identifying cynomorium songaricum of different processing modes and host plants based on an electronic tongue technology. The method comprises the following steps: weighing 1.0 g of a sample, adding 10mL of water, dissolving, filtering after 30min, repeating for three times, merging filtrate, obtaining a test solution, and detecting by using an electronic tongue; statistical analysis; the method has the beneficial effects or advantages of rapid and accurate quality identification, non-destructive detection, diversified processing modes and host plant identification, promotion of healthy development of the cynomorium songaricum industry, improvement of intelligent and automatic levels and the like. Due to the advantages, the method has wide application prospects and market potential in the field of cynomorium songaricum quality identification.

The invention discloses a botanical attractant for thrips hawaiiensis in a banana field and application of the botanical attractant. According to the application, alpha-pinene, caryophyllene, DL-limonene, beta-pinene and D-limonene which have a remarkable attracting effect on thrips hawaiiensis are separated and identified from banana flower buds which are host plants of the thrips hawaiiensis, and effective concentrations of the compounds with the attracting effect on the thrips hawaiiensis are screened out; the five compounds are mixed according to a certain volume ratio to prepare the thrips hawaiiensis luring composition. Indoor and outdoor experiments prove that the trapping composition provided by the invention has remarkable trapping activity on thrips hawaiiensis in a banana field. The attractant provided by the invention is derived from plants, is simple to prepare, is environment-friendly, is non-toxic to people and livestock, and has a good popularization and application prospect.

The invention discloses a Guangxi strigose hydrangea juvenile tea gall mite comprehensive control method based on ecological regulation and control, and belongs to the technical field of ecological planting of strigose hydrangea juvenile tea. The method comprises the steps that lemongrass is planted on the periphery to form a smell barrier, purple perilla is arranged in the middle, Guangxi strigose hydrangea juvenile tea and sugar oranges are planted at intervals in a core area, and a composite rhombic layout is formed; chinaroot greenbrier is planted in the interplanting zone at intervals to serve as gall mite trapping plants, and matrine or azadirachtin botanical pesticide is sprayed to the chinaroot greenbrier regularly; a composite substrate containing 60-65% of loess, 20-25% of bagasse biochar, 10-12% of vermiculite and 3-5% of sodium alginate-attapulgite clay composite water-retention slow-release particles is adopted to form a water-retention breathable layer with a slow-release function; and spreading wormwood seeds around the host plants to inhibit survival of overwintering adults of the gall mites. The method has good ecological benefits and insect pest prevention and control efficiency, effectively improves the soil microenvironment and is suitable for sweet tea planting in subtropical hilly areas such as Guangxi.

The invention discloses a VdNOB1 gene as well as an anti-pathogenic bacterium target gene segment, an interference vector and disease-resistant application thereof. According to the invention, the VdNOB1 gene is subjected to knockout mutation, so that the infection pathogenicity of verticillium dahliae to host plants can be obviously reduced. The invention further provides a verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment, the nucleotide sequence of the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment is shown as SEQ ID No.4, and the pathogenicity of verticillium dahliae to host plants can be remarkably reduced after plants are transformed by an RNA interference vector constructed by the verticillium dahliae VdNOB1 gene anti-pathogenic bacterium target gene segment. The invention further provides an RNA (Ribonucleic Acid) interference vector containing the VdNOB1 gene or an anti-pathogenic bacterium target gene fragment thereof. The verticillium dahliae VdNOB1 gene or the anti-pathogenic-bacterium target gene segment and the RNA interference vector thereof have application prospects in the aspects of improving the resistance of crops to diseases caused by verticillium dahliae, cultivating new varieties of verticillium dahliae-resistant plants and the like.

The invention relates to the field of microbial genetic transformation, and discloses a preparation method of Ilyonectria robus fungus protoplast and a construction method of a genetic transformation system.The preparation method comprises the following steps that S11, I.robus hyphae are prepared, after I.robus is cultured on a PDA solid culture medium for 14 days, the I.robus hyphae are inoculated to a CMC liquid culture medium to be subjected to shake-flask culture, and spores are filtered and collected; diluting the spore concentration, inoculating into a YEPD liquid culture medium, carrying out shake-flask culture, filtering and collecting hyphae; and S12, preparing enzymatic hydrolysate, namely adding the hyphae prepared in the step S11 into the enzymatic hydrolysate prepared in the step S12, carrying out shake flask enzymolysis, filtering, centrifuging filtrate, discarding supernate, adding a 0.7 M NaCl solution, centrifuging, and discarding the supernate, so as to obtain the I.robus fungus protoplast. The protoplasts with proper quantity and good quality can be efficiently obtained, and the transformation efficiency of a subsequent genetic transformation system is improved; and the constructed genetic transformation system can be used for construction of a mutant library of the I.robusta strain, interaction of a host plant and the I.robusta strain and research on the pathogenesis of the I.robusta strain.

The present invention discloses the use of a gibberellin-deficient mutant in promoting spore production in arbuscular mycorrhizal fungi. The present invention utilizes the hairy roots of the gibberellin-deficient mutant as a host plant to inoculate the arbuscular mycorrhizal fungi, significantly increasing the spore production of the arbuscular mycorrhizal fungi. The hairy roots of the gibberellin-deficient mutant can significantly increase the spore production of the arbuscular mycorrhizal fungi under both normal pH conditions and acidic conditions. The gibberellin-deficient mutant can be widely used to promote spore production in arbuscular mycorrhizal fungi. The present invention also provides a preparation and method for promoting spore production in arbuscular mycorrhizal fungi. The preparation and method can propagate the arbuscular mycorrhizal fungi under sterile conditions with high propagation efficiency, significantly increase the spore production of the arbuscular mycorrhizal fungi, are simple to operate, low in cost, and have a short cycle time, thus having broad prospects for promotion and application.

Disclosed are endophyte inoculant compositions, methods of making the compositions, methods of using the compositions, and physiologically altered plants treated with the compositions. The endophyte inoculant compositions may include one or more of endophyte strains WW5, WW6, WW7, and PTD1, which when applied to a non-native host plant, promote plant mineral nutrient acquisition and uptake, plant vigor, health, growth, and yield.

The invention relates to the technical field of traditional Chinese medicinal material production, in particular to a green prevention and control method for main leaf pests of tetranychus urticae and tetranychus crataegi in the under-forest ecological planting process of polygonatum sibiricum, and the method comprises the steps of S1, egg overwintering prevention and control, S2, non-host plant repelling, S3, biological agent killing, S4, agronomic measure blocking, and S5, late autumn tannin insect prevention and egg inhibition. Aiming at the technical problem of prevention and control of leaf mites of polygonatum sibiricum, from the perspective of sustainable development of traditional Chinese medicine ecological agriculture, an ecological technology is used in comprehensive treatment of leaf pests of polygonatum sibiricum, a green prevention and control implementation path for tetranychus urticae and tetranychus crataegus is provided, pollution of agricultural chemicals can be reduced, and the environment-friendly prevention and control of leaf mites of polygonatum sibiricum is realized. Meanwhile, green forest ecological traditional Chinese medicine products are produced, the rhizome yield per unit area is increased by 9.26%, it is ensured that the first-grade product rate is higher than that of conventional cultivation by 13.58% or above, and economic benefits are improved.

The invention discloses a large-scale tea lesser leafhopper cultivation and preservation method suitable for cicada tea production. The method comprises the following steps: step 1, preferably selecting Jinxuan, jade or Fuding white tea as a host plant variety, and determining the specification of the host plant; step 2, establishing a tea lesser leafhopper adult maintenance area, a nymph maintenance area and an egg incubation area, performing partition maintenance, and preferably selecting optimal maintenance conditions and culture density of different maintenance areas; step 3, preferably selecting a collection mode of tea lesser leafhopper eggs: preferably selecting an adult male-female ratio, and preferably selecting egg laying population density, egg collection duration and the like; 4, a tea lesser leafhopper egg storage chamber is established, and storage conditions are optimized; and step 5, preferably selecting incubation conditions and incubation time of the tea lesser leafhopper eggs after low-temperature storage. According to the large-scale production method, the tea branches to be trimmed in the tea garden are utilized, and the cost is low. The tea branches are used for culture, and the death rate caused by the fact that the leafhoppers need to adapt to host plants again during release is reduced.

The invention relates to the technical field of biology, and discloses an FPSE09041 protein and an application of a coding gene of the FPSE09041 protein in reducing pathogenicity of fusarium pseudograminearum. The amino acid sequence of the FPSE09041 protein is as shown in SEQ ID NO. 2, and the coding gene of the FPSE09041 protein is as shown in SEQ ID NO. 1. By knocking out the FPSE09041 gene, the expression of the FPSE09041 protein is inhibited, and the pathogenicity of the fusarium pseudograminearum to host plants is reduced. The research finds that the FPSE09041 is an indispensable key factor for pathogenicity of the fusarium pseudograminearum, and the survival and infection capabilities of pathogenic bacteria can be obviously weakened due to function deficiency of the FPSE09041. Therefore, the FPSE09041 protein and the coding gene thereof can be used as ideal molecular targets for developing novel, efficient and high-specificity antifungal agents, and a new technical path is provided for green prevention and control of wheat stem rot.

The invention provides a botanical fungicide of a chaulmoogra seed extract, which takes sub-chaulmoogra seed oleic acid as a marker, and application of the botanical fungicide in preparation of medicines for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The hydnocarpus anthelmintica extract is obtained by extracting hydnocarpus anthelmintica seeds with a plurality of different types of organic solvents such as ethanol, petroleum ether and the like and treating the seeds by a physical method, and the hydnocarpus anthelmintica extract contains sub-hydnocarpus anthelmintica oleic acid as markers in different proportions. The extract has the effects of inhibiting conidium germination, bud tube growth or appressorium formation of fungi and oomycetes, effectively preventing pathogenic bacteria from infecting host plants and preventing and treating important plant diseases such as rice blast, mango anthracnose, potato late blight and rice false smut.

The invention belongs to the technical field of gene engineering, and relates to an apple tree valsa ceratosperma virulence gene and application and dsRNA thereof. The invention discloses an apple tree valsa ceratosperma virulence gene VmNRPS25, the CDS region sequence of the apple tree valsa ceratosperma virulence gene VmNRPS25 is shown as SEQ ID NO: 1, the expression of the apple tree valsa ceratosperma virulence gene VmNRPS25 in V.mali is inhibited, the biomass of V.mali in a host plant is reduced, and the virulence of V.mali is weakened. The invention further constructs dsRNA on the basis of the valsa mali virulence gene VmNRPS25, and the dsRNA induces silencing of the valsa mali virulence gene VmNRPS25 in V.mali through an exogenous spraying method, and can be used for prevention and treatment of V.mali and pathogenic plant diseases thereof in host plants.

The invention relates to the technical field of biotechnology and crop disease prevention and control, in particular to application of PABP protein coded by solanaceae plants to prevention and control of tobacco mosaic virus viruses. The invention finds that the PABP protein (taking NbPABP as an example) encoded by solanaceae plants and the encoding gene thereof play an important role in infection and proliferation of the pepper mild mottle virus PMMoV, are susceptible genes of PMMoV encoded by host plants, can be used for preventing and treating pepper mild mottle virus diseases caused by PMMoV, and can be used for molecular breeding for resisting / resisting PMMoV and the like. Valuable protein is provided for antiviral infection of plants, particularly, the antiviral level of solanaceous plants can be improved, production safety of solanaceous vegetables is guaranteed and promoted, and the protein has wide application prospects in the fields of antiviral breeding of plants and the like.

The invention discloses a strain NJSH56 for promoting plant growth and application thereof, the strain NJSH56 is identified as bacillus pumilus, the strain NJSH56 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation time is November 14, 2024, and the strain preservation number is CGMCC NO.32614. The strain NJSH56 can promote plant growth. The dendrobium officinale endophytic growth-promoting strain NJSH56 for promoting plant growth is obtained through separation and screening, growth of dendrobium officinale tissue culture seedlings can be effectively promoted, and the fresh weight, the rooting number and the leaf number are increased; greenhouse tests show that the number of new buds of dendrobium officinale plants in a strain NJSH56 treatment group reaches 4.67 and is obviously higher than that of a control group; meanwhile, the NJSH56 is used for treating the non-host plant Chinese cabbage, so that the growth of the overground part of the Chinese cabbage can be remarkably promoted, and the biomass accumulation of the Chinese cabbage is improved.

Endophytic bacteria, compositions comprising the same, and methods of use thereof are disclosed which increase the root and shoot growth of grass hosts, suppress growth of soil borne fungal pathogens of host plants, increase resistance of the grass to diseases and reduce competitiveness of distantly related competitor weeds of the crop.

This invention discloses a deep learning-based method for predicting the phenological synchronization of specialized butterflies and their host plants, belonging to the interdisciplinary field of biodiversity conservation, ecological prediction, and deep learning technology. The method includes: collecting environmental climate variables, butterfly occurrence data, and phenological time-series data of their host plants; preprocessing the data to create training and validation sets; constructing a dual-label system with the day of year (DOY) of butterfly occurrence peaks (e.g., emergence peak, egg-laying peak) as the primary label and the normalized value of synchronization deviation as the secondary label; building a deep learning model with ecological weights, including an embedding layer, an LSTM layer, and a fusion layer; inputting a vector concatenated from daily or weekly climate time-series features and ecological dynamic features; and outputting the primary and secondary labels through a dual-output layer; designing a hybrid loss function that integrates the main regression loss, ecological synchronization constraint loss, and small-sample regularization loss; training the model using the Adam optimizer and implementing an early stopping mechanism; and validating and evaluating the model using regression accuracy and ecological synchronization accuracy indicators. The method includes modules for data acquisition, preprocessing, label construction, model training, prediction evaluation, and storage. This invention improves the accuracy of phenological synchronous prediction by embedding ecological dynamic feature weights and dual-label constraints, adapts to small sample scenarios, and provides technical support for the monitoring and protection management of rare butterfly species (such as the Golden Birdwing and the Chinese Tiger Swallowtail).

The invention relates to the technical field of citrus insect pest prevention and control, and discloses a comprehensive ecological prevention and control method for diaphorina citri. The method comprises the following steps: acquiring current insect pest state information based on real-time monitoring data of a citrus planting area; extracting diaphorina citri biological characteristics and associated ecological environment characteristics through the ecological characteristic library to form an initial ecological characteristic set; fusing the real-time monitoring data with the initial ecological feature set to generate a dynamic ecological feature set; calculating the priority score of each prevention and control strategy according to the spatial and temporal distribution density, migration trend intensity, natural enemy inhibition coefficient, host plant resistance index and environmental stress factor of each feature in the dynamic set; generating a strategy sorting table in a descending order according to the scores, sequentially selecting to-be-executed strategies, and judging whether the to-be-executed strategies complete the target population density reduction amount or not in a preset period. According to the method, real-time data and ecological characteristics are combined, the strategy is dynamically adjusted, and the prevention and control accuracy and effectiveness are improved.

Transgenic plants with blue flower color, or their inbred or outbred progeny, or their propagules, partial plant bodies, tissues or cells, are provided. A buckwheat-derived C-glucosyltransferase (CGT) gene or its homolog is transferred into a host plant to cause delphinidin-type anthocyanins and flavone mono-C-glycosides to be copresent in the plant cells.

The application discloses a LAMP primer group, a kit and a detection method for detecting Pinus armandii rust fungus, and belongs to the technical field of genetic engineering. The application takes the specific nucleotide sequence of the large subunit ribosomal RNA of the Pinus armandii rust fungus as a target gene to design and screen a primer group which is specific and high in sensitivity, and can be used for LAMP detection of the Pinus armandii rust fungus. The LAMP primer group provided by the application has the advantages of high universality, high specificity, high sensitivity and good accuracy, and the detection limit can reach 4.5x10 ‑4 ng / μL, which is 100 times higher than the sensitivity of the LAMP detection of different species in the same genus in the prior art, the detection method can rapidly detect the Pinus armandii rust fungus from spore piles and host plant materials, and is suitable for the detection of Pinus armandii seedling materials in forest fields and customs quarantine ports.

The invention discloses a diaphorina citri effector protein DcPDI1 and an application thereof. The amino acid sequence of the diaphorina citri effector protein DcPDI1 is as shown in SEQ ID NO. 1, and the nucleotide sequence of the diaphorina citri effector protein DcPDI1 is as shown in SEQ ID NO. 2. After the diaphorina citri DcPDI1 is knocked out, the egg laying amount and the developmental duration of the diaphorina citri are remarkably reduced, and it is indicated that the DcPDI1 plays an important role in growth and development of the diaphorina citri. The diaphorina citri effect protein DcPDI1 can be a new molecular target spot, plays a key role in activating defensive response of host plants and helping diaphorina citri to eat, and provides a new insight for a molecular mechanism of interaction between diaphorina citri and plants.

The present invention relates to an efficient method for artificially synthesizing Lactarius deliciosus mycorrhiza, which comprises the following steps: (1) Take the mycelium with a size of 1-3 mm at the connection of the stipe and cap of fresh Lactarius deliciosus fruit bodies, place it in a PDA medium, and conduct tissue culture at 20-25 °C for 20-30 days. Then, take the mycelium block and inoculate it onto the PDA medium, and continue to culture at 20-25 °C for 25-40 days. Subsequently, take the mycelium block again and inoculate it into a PDB medium for culturing for 30-50 days to obtain Lactarius deliciosus liquid spawn; (2) Transplant pine seedlings at a density of 6-15 seedlings per basket into a culture basket filled with mycorrhizal substrate, add the Lactarius deliciosus liquid spawn prepared in step (1) to the substrate 3-5 cm deep near the root of each seedling, and mycorrhiza can be formed after culturing for 10-14 days. The present invention scientifically screens the substrate and method for Lactarius deliciosus. By using the optimized medium formula and culture method, the mycelium of Lactarius deliciosus grows faster and the biomass of the obtained Lactarius deliciosus mycelium is larger; inoculating the plant roots with liquid spawn greatly improves the inoculation efficiency; multiple inoculated host plants are cultured in the same culture device, which greatly shortens the mycorrhiza formation time after inoculation and effectively improves the mycorrhiza synthesis rate; reducing the amount of peat soil in the mycorrhizal substrate reduces the proportion of mycorrhiza formed by miscellaneous bacteria and pine roots.

The invention discloses a solid-state fermentation process of plant endophytic actinomycetes OsiLf-2, a spore storage method and application. The solid-state fermentation process comprises the following steps: preparing a solid-state fermentation culture medium, inoculating a seed solution for solid-state fermentation, and collecting spores. The solid-state fermentation process disclosed by the invention can produce spores on a large scale, shortens the fermentation period, and has the advantages of simple process, convenience in operation, short fermentation period, low bacterial contamination rate, low raw material cost, high sporulation quantity and the like; the obtained spores can be freeze-dried under the protection of a skimmed milk powder solution to form spore powder, so that the freeze-drying survival rate and the long-term storage stability of the spores can be improved. The spore provided by the invention can be used for promoting crop growth, is more environment-friendly to ecology especially when being used for preparing a microbial fertilizer, does not influence the agricultural ecological environment and human body health, can promote the growth and development of host plants, and is high in use value and good in application prospect.

The invention discloses a protein capable of inducing a plant immune activation function and application of the protein in disease control, the gene sequence of the protein capable of inducing the plant immune activation function is shown as SEQ ID NO: 1, and the amino acid sequence of the protein is shown as SEQ ID NO: 2. According to the invention, a novel plant immune activator protein CgSsp16 secreted by colletotrichum gloeosporioides is identified, can be identified by plants, stimulates a plant immune response mechanism, triggers broad-spectrum resistance of host plants to various pathogens and improves disease resistance of various plants; the compound is expected to be developed into a novel biopesticide to be applied to green and sustainable production of agriculture.