Seed specific highly effective promoter and its application

A promoter and specific technology, applied in the application, the use of vectors to introduce foreign genetic material, biochemical equipment and methods, etc., can solve the problems of toxicity, different expression levels, hindering the normal growth of plants, etc.
CN101063139BInactive Publication Date: 2010-09-22CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2010-09-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a special promoter separated from millet, expressing carrier with nucleic acid sequence of SEQ ID No. 1 host with the expressing carrier and appliance of the promoter, which is characterized by the following: utilizing Tail-PCR (colored body step moving method); getting the special promoter from gene group DNA; possessing nucleic acid sequence of SEQ ID No. 1; ;linking downstream of the promoter to non-homologous or homologous gene; constructing plant expressing carrier; transferring host plant; driving the downstream gene to high effective and special express goal protein in the seed; realizing genetic modification of plant; or using as effective tool for studying plant and biological reactor.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a seed-specific high-efficiency promoter sequence isolated from millet and its application. The above-mentioned seed-specific promoter is cloned from millet genomic DNA, and connected with different homologous and heterologous genes to construct a plant expression vector, transforming a host plant, and enabling the transgenic plant seeds to efficiently and specifically express their downstream genes. Method and application. Background technique

[0002] During the research and development of transgenic plants, it was found that the low expression level of exogenous genes was often an important factor that resulted in the inability to obtain ideal transgenic plants and effectively study the mechanism of gene action. Since the key to affecting the expression level is the promoter upstream of the gene, the selection of a suitable plant promoter is the first consideration in enhancing th...

Claims

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