685 results about "Agarose" patented technology

The invention discloses a specific molecular marker, primers, a method and application for genetic sex identification of siniperca chuatsi, the specific molecular marker has two sequences in male fish and has one sequence in female fish, the primers comprise an upstream primer F and a downstream primer R. The primers are adopted to carry out PCR amplification on genome DNA of the siniperca chuatsi to be detected, and the specific molecular marker is obtained. And identifying the genetic sex of the siniperca chuatsi according to an agarose gel electrophoresis detection result. The invention further discloses application of a reagent, a primer, a kit or a method for detecting the specific molecular marker in genetic sex identification of siniperca chuatsi, artificial breeding of the siniperca chuatsi or all-female breeding of the siniperca chuatsi. The siniperca chuatsi genetic sex identification method has the advantages that sex-related male and female differential fragments are found through combined comparative analysis of male and female genomes, primers are designed for amplification verification, only a small amount of DNA of a to-be-detected sample genome is needed as a template, the genetic sex of the siniperca chuatsi can be accurately, simply, conveniently, quickly and stably identified only through PCR and agarose gel electrophoresis, and the method is not limited by the development stage of the siniperca chuatsi.

The invention discloses a multi-effect sodium hyaluronate composite gel, a preparation method thereof and application of the multi-effect sodium hyaluronate composite gel in cosmetology, and belongs to the technical field of plastic surgery and general surgery implants. The multi-effect sodium hyaluronate composite gel is prepared from sodium hyaluronate gel and agarose mixed gel. The multi-effect sodium hyaluronate composite gel prepared by the invention not only can moisturize skin and supplement collagen, has the effects of whitening, resisting oxidation and resisting inflammation, but also effectively solves the problem that sodium hyaluronate, collagen and PDRN are easy to degrade, so that the beautifying effect is more long-acting, and the effect is more obvious. Therefore, the implant has better application prospect and value in the fields of plastic and aesthetic surgery and general surgical implants.

The present invention discloses a method for separating and purifying recombinant human collagen, wherein the method first filters the recombinant human collagen fermentation broth, then uses cationic composite chromatography to crudely purify the recombinant human collagen to obtain a cationic composite chromatography eluent, and then uses a hydrophobic chromatography column to purify the purified recombinant human collagen, wherein the cationic composite chromatography column is filled with a high-flow rate agarose matrix containing a hydrophobic group of a phenyl group and an electrostatic group of a carboxyl group as a filler, and the hydrophobic chromatography column is filled with a hydrophilic polymethacrylate matrix containing a phenyl group as a filler. The final target protein purity of the present invention is greater than 99%, the recovery rate is greater than 85%, and the endotoxin content is less than 0.5EU / mg. The process of the present invention is simple and easy to operate, and the purification cost requirement is low. The recombinant human collagen obtained by the method has high purity and low endotoxin content, and meets the requirements of cosmetic raw materials, medical devices, and medical-grade high-purity collagen.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention discloses a fruit and vegetable exosome extraction method based on ligand fishing. The fruit and vegetable exosome extraction method comprises the following steps: suspending amino-functionalized Fe3O4 (at) SiO2 nanoparticles in a glutaraldehyde solution, and adding activated agglutinin to obtain Fe3O4 (at) SiO2-agglutinin nanoparticles; adding a glutaraldehyde solution into agarose gel to obtain a size exclusion chromatographic column filler; the method comprises the following steps: dispersing Fe3O4 (at) SiO2-lectin nanoparticles in a PBS buffer solution to obtain a uniform suspension, adding the uniform suspension into a size exclusion chromatographic column filler to obtain a uniform slurry, filling the size exclusion chromatographic column with the uniform slurry, and flushing with the buffer solution to form a magnetic bead stationary phase; and loading fruit and vegetable supernatant into a size exclusion chromatographic column, passing through the magnetic bead stationary phase, cleaning residual impurities combined on the exosome-magnetic bead compound by using a buffer solution, and dissociating the exosome from the magnetic beads by using a competitive eluent of the exosome to obtain the high-purity fruit and vegetable exosome. The problem that an existing exosome extraction method is difficult to meet the requirements of high efficiency, simplicity, high purity and high activity at the same time is solved.

The invention discloses a fluorescence ratio type carbon dot hydrogel sensor, a preparation method thereof and application of the fluorescence ratio type carbon dot hydrogel sensor in rapid detection of doxorubicin hydrochloride. The invention discloses a fluorescence ratio type carbon dot hydrogel sensor which is characterized in that 3-aminophenol is used as a carbon source, absolute ethyl alcohol is used as a solvent, carbon dots are prepared through a one-step solvent method, the carbon dots have green fluorescence, agarose is dissolved in a mixed solution of deionized water and a phosphate buffer solution to be fully dissolved, and then the carbon dots are prepared into the fluorescence ratio type carbon dot hydrogel sensor. And adding the carbon dots into the mixed solution, fully dispersing, and cooling to obtain the fluorescence ratio type carbon dot hydrogel sensor. The sensor disclosed by the invention has the characteristics of high selectivity and high sensitivity when being used for detecting doxorubicin hydrochloride, and also has the characteristics of convenience, visibility, rapidness, portability, easiness in operation and the like.

The invention provides a preparation method and application of medical grade agarose with different molecular weights, and belongs to the technical field of biomedical materials. According to the invention, the crude agarose product is treated by DMSO, EDTA-Na2 and DEAE-cellulose, and then the acid concentration, the acid treatment temperature, the acid treatment time and the like in the acid treatment stage are finely regulated, so that the medical-grade agarose with medium-high molecular weight (3000-100000), medium molecular weight (3000-3000) and low molecular weight (1t, 3000) is successfully prepared. The medium-high molecular weight and medium molecular weight medical grade agarose provided by the invention has excellent gel performance, adjustable mechanical strength and injection performance, and is suitable for the fields of skin implants, gel dressings, tissue engineering and the like; the low-molecular-weight medical grade agarose shows good water solubility, fluidity and oxidation resistance, and has significant application potential in the fields of medical skin care products and functional dressings.

The invention belongs to the technical field of fish genetic identification, discloses a multi-site large yellow croaker wild domestication population identification system based on an agarose gel electrophoresis technology, and particularly discloses a group of InDel molecular markers for large yellow croaker wild and domestication population identification, and the InDel molecular markers comprise InDel11-5053756, InDel20-14004876, InDel3-28345321 and InDel21-17327392. The InDel molecular marker detection primer is used for amplification to obtain a PCR product, agarose gel can be used for detecting strip polymorphism, extra restriction enzyme digestion or complicated polyacrylamide gel electrophoresis operation is not needed, and identification of large yellow croaker wild and domesticated populations can be rapidly and accurately achieved.

The invention discloses bioactive macroporous hydrogel as well as a preparation method and application thereof. The bioactive macroporous hydrogel comprises hydrogel particles and a bioactive load loaded on the hydrogel particles, the hydrogel particles are obtained by mechanically extruding a hydrogel matrix, and the hydrogel matrix is formed by compounding sodium alginate, agarose and methacrylated hyaluronic acid through hydrogen bond crosslinking and photo-crosslinking; the bioactive load comprises: a) small extracellular vesicles of which the surfaces are modified with macrophage targeted CRV peptides; and b) a PLGA (poly (lactic-co-glycolic acid)) microsphere loaded with a cartilage inducer Kartogenin. The bioactive macroporous hydrogel disclosed by the invention has an internally communicated macroporous network, is beneficial to cell infiltration, blood vessel ingrowth and nutrient substance diffusion, and overcomes the defects that the traditional hydrogel is small in pore size and limits cell behaviors. According to the bioactive macroporous hydrogel disclosed by the invention, dual bioactive loads are adopted to synergistically promote tendon-bone healing, so that the treatment effect is better.

The invention discloses a method for efficiently leaching waste battery positive electrode powder by using hydrogel to assist bacteria, belonging to the field of resource recovery technology. The invention utilizes hydrogel to assist bacteria to leach valuable metal elements from waste LIBs. The bacteria include L. plants and / or L. acidophilus , the hydrogel is prepared by polyvinyl alcohol and agarose. The present invention creatively applies hydrogel in the bioleaching process, effectively increases the leaching percentage of valuable metals in waste LIBs, and avoids the use of inorganic acid at the same time. Compared with the traditional chemical leaching technology, the method of the present invention has obvious advantages in operational safety and effectively avoids the risk of secondary pollution to the environment. By using microorganisms, not only the efficient recovery of metal resources in the battery is achieved, but also the environmental friendliness of the whole process is ensured.

The invention belongs to the technical field of plant fungus molecular biology detection, and discloses an LAMP primer group, a kit and a detection method for detecting oat smut pathogenic bacteria. A group of LAMP (loop-mediated isothermal amplification) specific primers are designed according to a specific sequence on a whole genome of the oat smut pathogenic bacteria Ustilago hordei, results are judged through a real-time fluorescence quantification method, an agarose gel electrophoresis method and an SYBR Green I fluorescent dye developing method, and the oat smut pathogenic bacteria carried by oat seeds are detected. The LAMP detection method for the oat smut pathogenic bacteria, established by the invention, is strong in specificity, high in sensitivity, high in speed and low in cost, provides a new technical means for detection of the oat smut pathogenic bacteria carried by the oat seeds, and has relatively high practical application value.

The invention discloses a device and method for automatically shearing and recycling agarose gel, and relates to the technical field of agarose gel cutting, and the device and method for automatically shearing and recycling agarose gel comprise an image acquisition unit, a three-axis moving mechanism, a gel cutting device, an electrophoresis apparatus and a control unit; the image acquisition unit comprises a light source assembly and an image acquisition camera corresponding to an irradiation area, the image acquisition camera is used for shooting a dyed electrophoresis image, and the light source assembly is used for obliquely irradiating gel to enable a sample strip to develop color; the control unit comprises an analysis processing unit; the analysis processing unit comprises a deep learning detection module and a data processing module; the control unit cooperates with the image acquisition unit, the three-axis moving mechanism and the gel cutting device, so that a complete automatic process from strip identification and positioning after electrophoresis to gel block cutting, grabbing, releasing and recycling is constructed.

The present application discloses a specific DNA fragment for sex identification of Pelteobagrus ussuriensis and a method for sex identification, specifically relating to the technical field of sex identification of Pelteobagrus ussuriensis. The above-mentioned identification method includes the following steps: Dissect 5 female and 5 male Pelteobagrus ussuriensis of a full-sib family to determine the physiological sex; respectively extract DNA from the muscles of female samples and male samples, construct a paired-end genomic DNA library with an insert fragment size of 300 bp, and perform high-throughput sequencing; based on the GWAS and Fst methods, identify that the sex chromosome of Pelteobagrus ussuriensis is chromosome 8, and then screen for Indels within the sex determination region on chromosome 8; screen a 601-bp male-specific DNA sequence and design and synthesize primers; use PCR amplification and agarose gel electrophoresis detection, and the result without specific bands is female, and the one with a 356-bp band is male. This scheme has the advantages of strong specificity, simple primer composition, and more intuitive identification of the sex of Pelteobagrus ussuriensis.

The invention provides a recombinant III-type humanized collagen gel based on a macromolecular crowding effect as well as a preparation method and application of the recombinant III-type humanized collagen gel. The method comprises the following steps: firstly, preparing an rhCol III solution system; then preparing a macromolecular crowding bath containing dextran 70, polyethylene glycol 8000 and ascorbic acid; then adding an rhCol III solution into the macromolecular crowding bath to form an rhCol III microgel; and finally, constructing a 3D printing molecular exclusion mold system which takes agarose as a substrate and contains a functional additive and a temperature-sensitive polymer as a temperature-sensitive release and spatial constraint component, so as to realize directional construction of the rhCol III microgel. According to the method disclosed by the invention, second-level physical gelling (lt; 15s) of rhCol is realized, and a physiological microenvironment supporting cell proliferation is constructed. The recombinant III-type humanized collagen gel prepared by the method can be used for preparing collagen microgel balls, collagen bundles, 3D (three-dimensional) bio-printing products and the like, and is wide in application range.

The present invention discloses a preparation method of Inonotus hispidus polysaccharide IHP-1aa: It is obtained by removing fat with ethanol, extracting with water and precipitating with ethanol, then removing proteins by the Sevage method, separating neutral polysaccharide components through a DEAE-52 cellulose column and a DEAE agarose gel column, and finally purifying with Sephadex G-100. The Inonotus hispidus polysaccharide has uniform purity and clear composition, and is a heteropolysaccharide composed of glucose, galactose, mannose, fucose and methylgalactose, with a molecular weight of 26.9 kDa. In vitro experiments show that it has no adverse effects on normal cells, can significantly stimulate macrophages RAW264.7 to secrete NO, IL-6 and TNF-α, has good immunomodulatory activity, and can be used for the development of immunomodulatory products.

The invention belongs to the technical field of cosmetics, and particularly relates to a water-in-oil-in-water three-phase coated microbead as well as a preparation method and application thereof. The water-in-oil-in-water three-phase coated microbead is of a spherical structure and sequentially comprises an outer water phase, an inner oil phase and an inner water phase from outside to inside, the outer water phase comprises sodium alginate, agarose, cyclodextrin, glycerin and other components, the inner oil phase comprises caprylic / capric triglyceride, grape seed oil, olive squalane, tocopherol and other components, and the inner water phase comprises sodium alginate, agarose, cyclodextrin, glycerin and other components. And the inner water phase comprises ampelopsis grossedentata extract, astragalus membranacus root extract and other components. According to the invention, an easily oxidized and unstable active matter is fully and firmly protected by adopting a droplet microfluidic coating technology, so that the active matter is free from external stimulation, active ingredients with different effects play multiple roles, and the composition has good anti-inflammatory, soothing and oil-controlling effects and can be applied to the field of cosmetics.

The invention discloses light-driven toxin-enriched composite hydrogel as well as a preparation method and application thereof. The composite hydrogel comprises light-driven hydrogel and detection hydrogel, the light-driven hydrogel is agarose hydrogel doped with Au (at) Ag core-shell nano particles; the detection hydrogel is a double strand formed by an okadaic acid aptamer and a complementary sequence cDNA thereof, and a double strand formed by a domoic acid aptamer and a complementary sequence DNAzyme thereof, the hairpin H1 is used for modifying a quenching group BHQ2 at the 3'end, the hairpin H2 is used for modifying a fluorophore Cy3 at the neck part, the hairpin H3 is used for modifying a fluorophore FAM and a quenching group BHQ1 at the two ends respectively, and the metal ion doped agarose hydrogel is used for catalyzing DNAzyme cyclic shearing; and the photo-thermal driving unit is positioned on the detection function unit. According to the present invention, with the composite system of upper layer photo-thermal enrichment and lower layer dual-signal detection, the rapid and high-sensitivity simultaneous detection of the okadaic acid and the domoic acid is achieved through the combination of the photo-thermal transpiration effect and the HCR and DNAzyme cyclic shearing reaction;

The invention is applicable to the technical field of polymer biomedical hydrogel materials, and provides a preparation method and application of multifunctional agarose-based particle composite hydrogel based on liquid-liquid phase separation, and the agarose-based particle composite hydrogel is a product formed by introducing microsphere particles into an agarose hydrogel body matrix; the microsphere particles are formed by a prepolymer and sodium lignin sulfonate through phase separation mediated by a composite behavior based on non-covalent interaction; the prepolymer is obtained by carrying out amine-epoxy reaction on an amino compound and an epoxy compound in water. According to the prepared agarose-based particle composite hydrogel, a microsphere particle structure is formed on the surface of an agarose matrix while positively charged amino groups and sodium lignin sulfonate are introduced, so that the multifunctional agarose-based particle composite hydrogel is prepared.

The invention provides a high-flux porous microsphere for chromatography and a preparation method thereof, and the preparation method of the high-flux porous microsphere for chromatography comprises the following steps: respectively dissolving agarose and aminated glucan in water, and uniformly mixing an agarose solution and an aminated glucan aqueous solution to obtain an aqueous phase; adding the water phase into the oil phase containing the emulsifier, and stirring and emulsifying at 70-90 DEG C for 30-90 minutes to obtain a mixed solution; cooling the obtained mixed solution to 20 DEG C or below at a speed of 1-5 DEG C / min, and curing into a ball to obtain a microsphere; and adding NHS-PEG-COOH into the obtained microspheres, carrying out a primary cross-linking reaction, adding epoxy chloropropane into the obtained microspheres, and carrying out a secondary cross-linking reaction to obtain the high-flux porous microspheres for chromatography. According to the preparation method of the high-flux porous microspheres for chromatography, the separation efficiency of the obtained porous microspheres can be improved, and relatively high dynamic adsorption capacity can still be realized at a high flow rate.

The invention provides an adsorption material and a preparation method and application thereof, the adsorption material comprises a carrier and an endotoxin affinity binding ligand, the endotoxin affinity binding ligand comprises a recombinant C factor protein, and the carrier and the recombinant C factor protein are connected through a spacer arm; wherein the carrier comprises at least one of resin, chitosan, cellulose, agarose and glucan; the spacer arm comprises at least one of modified polyethylene glycol, polyvinyl pyrrolidone, polymethylacrylic acid-2-hydroxyethyl ester, polyvinyl alcohol, polyacrylic acid, hexamethylenediamine, divinyl sulfone and glutaraldehyde, and at least one end of the modified polyethylene glycol is provided with carboxyl, aldehyde or amino. The adsorption material provided by the invention can efficiently adsorb endotoxin, has the characteristics of low toxicity risk and excellent blood compatibility, and can improve the safety of blood purification.

The invention discloses KASP primers for identifying genotypes of chicken green-shell eggs and application of the KASP primers. Compared with a common flow of identifying genotypes through PCR (Polymerase Chain Reaction) and agarose gel electrophoresis, the method for performing KASP genetic typing by utilizing the primers with the sequences as shown in SEQ ID NO: 1-3 provided by the invention has the advantages that the steps of preparing agarose gel and performing electrophoresis are omitted, and the economic cost is reduced; when large-scale detection is carried out, a large amount of manpower resources are saved, and the detection time is shortened; analysis is directly carried out according to an instrument detection result, personal errors are reduced, and the accuracy of a typing result is improved.

The invention discloses a degradable preservative film and a preparation method thereof. The film takes nano chitosan / agarose in a ratio of 1: 1 as a matrix, contains 0.5-5wt% of PVA (Polyvinyl Alcohol), 1-4wt% of protein powder or starch, 0.2-3wt% of tea polyphenol / carvacrol, 5-20wt% of glycerol and optional 5-15wt% of polylactic acid, and is cross-linked with sodium alginate. And carrying out tape casting at 55-65 DEG C, and carrying out gradient drying at 40-50 DEG C, thereby obtaining the product. The film is compact and flexible, the breaking strength is larger than or equal to 69 MPa, the oxygen permeability is low, the inhibition zone on six germs is enlarged by 3-4 mm, 98% of natural soil is degraded 20 days later, and no toxic smoke is generated during incineration. The biodegradable film is solvent-free in preparation, can be produced by existing equipment, is lower than a traditional biodegradable film in cost, can be in direct contact with food, remarkably prolongs the fresh-keeping period of fruits and vegetables, replaces PE and PVC, and solves white pollution.

The invention relates to a specific primer pair for sex identification of Chinese softshell turtles and an identification method thereof.The primer pair comprises two pairs of primers which are used for amplifying specific sequences in genome DNA of the Chinese softshell turtles respectively so as to achieve accurate sex identification, and according to the method, the genome DNA of a to-be-detected sample can be amplified through a PCR amplification reaction; when the amplification product is 1000 bp, the sex is judged, when the amplification product is 1000 bp and 2000 bp, the sex is judged, according to the identification method provided by the invention, standard PCR amplification conditions and a reaction system are adopted, high efficiency and accuracy of sex identification are ensured, the invention further provides a kit, and the kit can be used for detecting the sex. The kit comprises a reagent for extracting DNA (deoxyribonucleic acid), a primer combination and a reaction system required by PCR (polymerase chain reaction) amplification as well as an electrophoretic buffer solution and a labeling reagent required by agarose gel electrophoresis, is beneficial to improving the sex identification efficiency and accuracy of the Chinese softshell turtles, and is widely applicable to sex screening and other related researches in the aquaculture industry.

The invention discloses a double Taqman detection probe primer group for trichina and sarcocystis and application of the double Taqman detection probe primer group. Relates to the technical field of parasite detection. The invention provides a detection probe primer group, a kit and application. According to the detection method established by the invention, an amplification curve has a good linear relationship; the sensitivity is high; the specificity is high; dNA detection on 12 parasites such as toxoplasma gondii, ascaris suum, cryptosporidium and pulmonary nematode does not have an amplification curve; agarose gel electrophoresis shows that the molecular mass of the obtained PCR product strip accords with expectation. The kit has the characteristics of simplicity in operation, high specificity and high sensitivity, can quickly and efficiently detect trichina and sarcocystis, and has guiding significance in quick laboratory diagnosis of infection of the trichina and sarcocystis.

The invention discloses a toning lotion composition, and belongs to the technical field of cosmetics. The toning lotion composition is prepared from the following preparation raw materials in percentage by mass; 0.09%-0.23% of a thickening agent, 2%-9% of an oily component, 0.1%-2% of a stabilizing agent and the balance of water; the thickening agent is prepared from agarose, gellan gum and xanthan gum; the stabilizing agent is selected from at least one of sodium chloride, calcium chloride or disodium EDTA (Ethylene Diamine Tetraacetic Acid); the viscosity of the toning lotion composition at the temperature of 25 DEG C is 50 to 500 Pa.s. The toning lotion has fresh and cool use feeling and moist degree of oil, is high in transparency and flexible in use mode, can be used as essence for smearing and can also be atomized through a spray bottle.

The invention discloses ultrasonic responsive antibacterial hydrogel as well as a preparation method and application thereof. The ultrasonic responsive antibacterial hydrogel is prepared from the following raw materials: polyvinyl alcohol, agarose, barium titanate nanoparticles and water, the mass ratio of the polyvinyl alcohol to the agarose to the barium titanate nanoparticles to the water is 1: (0.25-4): (0.1875-0.75): (4-5). The hydrogel material disclosed by the invention not only can provide a moist environment required by a wound, but also can generate active oxygen under the ultrasonic action, so that wound healing is promoted, infection is prevented, and the rehabilitation process is accelerated.

The embodiment of the invention discloses an angiotensin II-1 type receptor polypeptide or a fusion compound and application of the angiotensin II-1 type receptor polypeptide or the fusion compound. The invention provides an angiotensin type 1 receptor polypeptide or fusion, which is coated in a solid phase material (porous plate, magnetic beads, agarose particles, latex particles and the like) and is used for detecting an antibody aiming at an angiotensin II-1 receptor in the body of a patient suffering from diseases such as eclampsia and the like. The compound can also be used for blocking the combination of autoantibodies in the bodies of pregnant women and angiotensin II-1 type receptors, and treating various cardiovascular diseases such as dilated cardiomyopathy, just-gauge disease, heart failure and the like.

The invention discloses an internal reference composition, a kit and a method for detecting the absolute content of microorganisms based on high-throughput sequencing, the internal reference composition comprises quantitative internal references P1-P12 and an indication internal reference P0, and the sequences of the quantitative internal references P1-P12 are shown as SEQ ID NO: 1-12; the sequence for indicating the internal reference P0 is as shown in SEQ ID NO: 13. The method comprises the following steps: adding an internal reference composition consisting of internal references with different concentration gradients into a detection sample for amplification together, carrying out gel leakage detection on amplicons by adopting agarose gel, carrying out gel electrophoresis analysis on a gel chart by using software, carrying out concentration comparison on PCR products, pre-estimating the internal reference proportion according to the concentration of a gel leakage strip, and determining the content of the internal reference in the PCR products. According to the method, samples with the internal reference pre-estimation proportion being 8%-50% are conveniently selected, the method is wide in application range, low in cost and high in accuracy, different types of samples can be covered, and the applicable sample concentration range is 1 ng / mu L-50 ng / mu L.

The invention relates to porous ceramic for a high-air-permeability semiconductor device as well as a preparation method and application of the porous ceramic, raw materials comprise 35-55 wt% of alumina powder and 10-55 wt% of a polymer microsphere pore-forming agent, and the polymer microsphere pore-forming agent comprises one or more of agarose, polylactic acid, polyacrylamide and organic silicon resin. The porous ceramic for the high-air-permeability semiconductor device, which is obtained by the preparation method provided by the invention, has relatively high porosity and air permeability, and also has relatively high mechanical strength and dielectric strength. According to the present invention, the gas volume flow rate is applied to the internal pore size characterization, and the high porosity and the high gas permeability can be achieved by accurately measuring the gas flow transmittance, accurately characterizing the pore size and the opening degree in the porous ceramic, and adjusting the particle size and the addition amount of the polymer microsphere pore forming agent; through the preparation process, the porosity and mechanical strength of the porous ceramic for the high-air-permeability semiconductor device can be balanced, meanwhile, high air permeability and high compressive strength are obtained, and the porous ceramic is particularly suitable for electrostatic chucks of different manufacture procedures.

The invention discloses an InDel molecular marker related to seedless traits of filial generations of grapes and application of the InDel molecular marker. The nucleotide sequence deletion part of the InDel molecular marker for the seedless type of the filial generation of seedless grape and nucleated grape is shown as SEQ ID NO.3, and is positioned at the upstream 263-693 bp of a VviAGL11 gene transcription start site (TSS) which is closely linked with the seedless character of the grape, and the nucleotide sequence deletion part of the InDel molecular marker is shown as SEQ ID NO.6. The nucleotide sequence deletion part of the InDel molecular marker is shown as SEQ ID NO.7. The nucleotide sequence deletion part is shown as SEQ ID NO.8. According to the invention, PCR (Polymerase Chain Reaction) amplification and Sanger sequencing are carried out on VviAGL11 gene promoters of a seedless grape variety 'Zhongqing 18' and a seedless grape variety 'Musang', so that polymorphic sites with significant difference are obtained, and the difference between the polymorphic sites is 431 bp. The InDel molecular marker is suitable for grape seedless molecular marker-assisted selective breeding, and the invention also discloses a method for carrying out genotype identification through an agarose electrophoresis technology in a seedling stage of hybrid generations of seedless grapes and seedless grapes, so that early selection of seedless characters of the grapes is realized; the seedless grape breeding efficiency is further improved, the breeding cost is reduced, and the breeding process is promoted.