940 results about "Agarose" patented technology

The invention discloses a specific molecular marker, primers, a method and application for genetic sex identification of siniperca chuatsi, the specific molecular marker has two sequences in male fish and has one sequence in female fish, the primers comprise an upstream primer F and a downstream primer R. The primers are adopted to carry out PCR amplification on genome DNA of the siniperca chuatsi to be detected, and the specific molecular marker is obtained. And identifying the genetic sex of the siniperca chuatsi according to an agarose gel electrophoresis detection result. The invention further discloses application of a reagent, a primer, a kit or a method for detecting the specific molecular marker in genetic sex identification of siniperca chuatsi, artificial breeding of the siniperca chuatsi or all-female breeding of the siniperca chuatsi. The siniperca chuatsi genetic sex identification method has the advantages that sex-related male and female differential fragments are found through combined comparative analysis of male and female genomes, primers are designed for amplification verification, only a small amount of DNA of a to-be-detected sample genome is needed as a template, the genetic sex of the siniperca chuatsi can be accurately, simply, conveniently, quickly and stably identified only through PCR and agarose gel electrophoresis, and the method is not limited by the development stage of the siniperca chuatsi.

The invention discloses a primer pair and a method for identifying a new Oudemansiella oosporoides strain HCS, and belongs to the technical field of specific molecular markers, the preservation number of the new Oudemansiella oosporoides strain HCS is CGMCC NO.41593, and the sequences of the primer pair Y6-F1 / Y6-R2 are as shown in SEQ ID NO.2 and SEQ ID NO.3. The specific method comprises the following steps: extracting DNA of an Oudemansiella oosporoides strain to be detected; carrying out PCR (Polymerase Chain Reaction) amplification by taking the extracted DNA of the sample to be detected as an amplification template and a molecular specific labeled primer consisting of the sequence shown by the primer as an amplification primer; and carrying out agarose gel electrophoresis detection on the PCR product. The primer pair has the advantages that the primer pair can be used for quickly identifying the strain HCS, the operation is simple and convenient, the specificity is strong, the accuracy is high, and a technical basis is provided for protecting a new variety of the strain HCS.

The invention relates to a polypropylene insulated power cable and a preparation method thereof, and belongs to the technical field of cables. The polypropylene insulation power cable comprises a copper conductive wire core, a metal shielding layer and a sheath layer, the metal shielding layer comprises a conductor shielding layer, a polypropylene insulation layer and an insulation shielding layer which are arranged from inside to outside, and the sheath layer comprises polypropylene, organic modified graphene, lignite wax, an antioxidant and an anti-UV agent; graphene and agarose form a firm covalent bond compound, then glycidyl methacrylate and eugenol are used for coating the outer layer of a graphene / agarose particle core, the dispersity and compatibility of graphene in a system are remarkably improved, and then the tensile strength and elongation at break of a polypropylene sheath layer material are remarkably improved; therefore, the polypropylene insulated power cable has excellent mechanical properties.

The invention discloses a multi-effect sodium hyaluronate composite gel, a preparation method thereof and application of the multi-effect sodium hyaluronate composite gel in cosmetology, and belongs to the technical field of plastic surgery and general surgery implants. The multi-effect sodium hyaluronate composite gel is prepared from sodium hyaluronate gel and agarose mixed gel. The multi-effect sodium hyaluronate composite gel prepared by the invention not only can moisturize skin and supplement collagen, has the effects of whitening, resisting oxidation and resisting inflammation, but also effectively solves the problem that sodium hyaluronate, collagen and PDRN are easy to degrade, so that the beautifying effect is more long-acting, and the effect is more obvious. Therefore, the implant has better application prospect and value in the fields of plastic and aesthetic surgery and general surgical implants.

The present invention discloses a method for separating and purifying recombinant human collagen, wherein the method first filters the recombinant human collagen fermentation broth, then uses cationic composite chromatography to crudely purify the recombinant human collagen to obtain a cationic composite chromatography eluent, and then uses a hydrophobic chromatography column to purify the purified recombinant human collagen, wherein the cationic composite chromatography column is filled with a high-flow rate agarose matrix containing a hydrophobic group of a phenyl group and an electrostatic group of a carboxyl group as a filler, and the hydrophobic chromatography column is filled with a hydrophilic polymethacrylate matrix containing a phenyl group as a filler. The final target protein purity of the present invention is greater than 99%, the recovery rate is greater than 85%, and the endotoxin content is less than 0.5EU / mg. The process of the present invention is simple and easy to operate, and the purification cost requirement is low. The recombinant human collagen obtained by the method has high purity and low endotoxin content, and meets the requirements of cosmetic raw materials, medical devices, and medical-grade high-purity collagen.

The invention belongs to the technical field of biomolecules, and particularly relates to an NIPBL gene for judging the sex of chickens and application of the NIPBL gene. The NIPBL gene has a segment with the difference of 606bp on chicken Z and W chromosomes, and is also a deletion segment on the W chromosome, the chicken sex can be quickly and effectively identified by detecting the sequence condition, the chicken with the deletion of the molecular marker sequence segment is a female individual, and the chicken without the deletion of the molecular marker sequence segment is a male individual; the invention further provides a specific primer, the chicken sex can be accurately judged through a PCR amplification technology and agarose gel electrophoresis, a strip is clear, the method is small in technical difficulty, easy and convenient to operate and high in accuracy, the sex of chicks or chickens difficult to distinguish in appearance can be effectively identified, the method has practical significance on identification of varieties small in male and female appearance difference, and the method is worthy of popularization and application. The method disclosed by the invention is strong in technical applicability and good in repeatability, can be used for accurately identifying the gender of the poultry, and has a wide application prospect.

The invention belongs to the technical field of sesbania genetic breeding, and particularly relates to a molecular marker for breeding or auxiliary breeding of stem tumor sesbania and application of the molecular marker in cross breeding and haploid breeding. The molecular marker provided by the invention is a set of InDel molecular markers for detecting the genetic similarity between the sesbania variety and germplasm and the stem tumor sesbania, and is distributed on the first, third, fourth, fifth and sixth chromosomes of the sesbania. The haplotype of the InDel molecular marker has great difference between the stem tumor sesbania and the core germplasm and variety of the sesbania, and is stably inherited. According to the invention, a primer pair for specifically amplifying the molecular marker is developed according to the difference of the molecular marker between stem tumor sesbania and root nodule sesbania, and by utilizing the primer pair provided by the invention, only the sesbania genome DNA needs to be extracted and then PCR amplification and agarose gel electrophoresis are carried out; therefore, whether the heredity source of sesbania is stem tumor sesbania can be rapidly and accurately identified or identified in an auxiliary manner.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination related to corn kernel traits and application thereof, and belongs to the technical field of molecular genetics. The SNP molecular marker related to the corn hundred-grain weight is developed by researching the relation between the Zm00001d014723 gene and the corn grain weight, early prediction of the yield of the selfing line is further achieved through PCR amplification and agarose gel electrophoresis and through direct and rapid identification of seedlings, and a new thought is provided for molecular breeding.

The invention relates to the technical field of crop molecular genetic markers, and discloses an InDel molecular marker of avena nuda and application thereof.The molecular marker is a group of 314bp deletion variation and 2bp insertion variation in the 610439484-610440026 interval of the 5C chromosome position of a reference genome of an avena nuda variety OT3098, the nucleotide sequence of the deletion variation is shown as SEQ ID NO.1, the nucleotide sequence of the insertion variation is shown as SEQ ID NO.2, and the nucleotide sequence of the insertion variation is shown as SEQ ID NO.3. The nucleotide sequence of the insertion variation is 5 '-AA-3'. The molecular marker provided by the invention can realize stable detection in agarose gel electrophoresis by combining a specific primer pair (SEQ ID NO.2-3) with an optimized touchdown PCR (Polymerase Chain Reaction) program, has the advantage of being not influenced by a tissue part and a growth period, and provides an efficient and reliable technical means for oat germplasm identification, seed purity detection and molecular breeding.

The invention discloses a fruit and vegetable exosome extraction method based on ligand fishing. The fruit and vegetable exosome extraction method comprises the following steps: suspending amino-functionalized Fe3O4 (at) SiO2 nanoparticles in a glutaraldehyde solution, and adding activated agglutinin to obtain Fe3O4 (at) SiO2-agglutinin nanoparticles; adding a glutaraldehyde solution into agarose gel to obtain a size exclusion chromatographic column filler; the method comprises the following steps: dispersing Fe3O4 (at) SiO2-lectin nanoparticles in a PBS buffer solution to obtain a uniform suspension, adding the uniform suspension into a size exclusion chromatographic column filler to obtain a uniform slurry, filling the size exclusion chromatographic column with the uniform slurry, and flushing with the buffer solution to form a magnetic bead stationary phase; and loading fruit and vegetable supernatant into a size exclusion chromatographic column, passing through the magnetic bead stationary phase, cleaning residual impurities combined on the exosome-magnetic bead compound by using a buffer solution, and dissociating the exosome from the magnetic beads by using a competitive eluent of the exosome to obtain the high-purity fruit and vegetable exosome. The problem that an existing exosome extraction method is difficult to meet the requirements of high efficiency, simplicity, high purity and high activity at the same time is solved.

The invention discloses a fluorescence ratio type carbon dot hydrogel sensor, a preparation method thereof and application of the fluorescence ratio type carbon dot hydrogel sensor in rapid detection of doxorubicin hydrochloride. The invention discloses a fluorescence ratio type carbon dot hydrogel sensor which is characterized in that 3-aminophenol is used as a carbon source, absolute ethyl alcohol is used as a solvent, carbon dots are prepared through a one-step solvent method, the carbon dots have green fluorescence, agarose is dissolved in a mixed solution of deionized water and a phosphate buffer solution to be fully dissolved, and then the carbon dots are prepared into the fluorescence ratio type carbon dot hydrogel sensor. And adding the carbon dots into the mixed solution, fully dispersing, and cooling to obtain the fluorescence ratio type carbon dot hydrogel sensor. The sensor disclosed by the invention has the characteristics of high selectivity and high sensitivity when being used for detecting doxorubicin hydrochloride, and also has the characteristics of convenience, visibility, rapidness, portability, easiness in operation and the like.

The invention belongs to the technical field of biomolecules, and particularly relates to an SMAD7 gene for judging chicken sex and application. Through the molecular marker and the design of a new PCR primer, the migration distance difference of two bands amplified by a ZW chromosome is 299bp, and compared with the migration distance difference of 150bp of a traditional sex identification gene CHD1 amplification system, the migration distance difference of the bands of the two items is larger, and judgment is easier; the fragments amplified by the designed PCR primer are smaller and are only 517bp and 816p, the PCR amplification time is remarkably shortened, and the sex identification time is shortened; in addition, amplification and agarose gel electrophoresis can be directly carried out by extracting chicken tissues, the sex of the chicken can be rapidly judged according to an electrophoresis result, the DNA extraction time is shortened, the time length can be controlled within 1 hour, operation is easy, the identification result is accurate, basic-level application and popularization are facilitated, and the method has a wide market application prospect.

The invention provides a preparation method and application of medical grade agarose with different molecular weights, and belongs to the technical field of biomedical materials. According to the invention, the crude agarose product is treated by DMSO, EDTA-Na2 and DEAE-cellulose, and then the acid concentration, the acid treatment temperature, the acid treatment time and the like in the acid treatment stage are finely regulated, so that the medical-grade agarose with medium-high molecular weight (3000-100000), medium molecular weight (3000-3000) and low molecular weight (1t, 3000) is successfully prepared. The medium-high molecular weight and medium molecular weight medical grade agarose provided by the invention has excellent gel performance, adjustable mechanical strength and injection performance, and is suitable for the fields of skin implants, gel dressings, tissue engineering and the like; the low-molecular-weight medical grade agarose shows good water solubility, fluidity and oxidation resistance, and has significant application potential in the fields of medical skin care products and functional dressings.

The invention provides an SSR (Simple Sequence Repeat) marker primer group and a detection method for identifying filial generations of hibiscus varieties, and belongs to the technical field of molecular markers, and the SSR marker HBG18 is used for carrying out PCR (Polymerase Chain Reaction) detection on a male parent'hibiscus rosa 'and female parent'chiffon' hybrid combination and a male parent'blue bird 'and female parent'Han-plus' hybrid combination offspring; the SSR marker HBG7 is used for carrying out PCR detection on the hybridized combination of the male parent pink heart double petals and the female parent Chinese chiffon; the SSR marker HBG21 is used for carrying out PCR detection on a hybridized combination of a male parent'common powder 'and a female parent'red heart' and a hybridized combination of a male parent'blue chiffon 'and a female parent'long bract double petal', agarose gel electrophoresis detection shows that the three SSR markers can accurately distinguish hibiscus parents and hybrid filial generations of the hibiscus parents, and the purpose of rapidly identifying the hybrid F1 generation is achieved.

The invention belongs to the technical field of fish genetic identification, discloses a multi-site large yellow croaker wild domestication population identification system based on an agarose gel electrophoresis technology, and particularly discloses a group of InDel molecular markers for large yellow croaker wild and domestication population identification, and the InDel molecular markers comprise InDel11-5053756, InDel20-14004876, InDel3-28345321 and InDel21-17327392. The InDel molecular marker detection primer is used for amplification to obtain a PCR product, agarose gel can be used for detecting strip polymorphism, extra restriction enzyme digestion or complicated polyacrylamide gel electrophoresis operation is not needed, and identification of large yellow croaker wild and domesticated populations can be rapidly and accurately achieved.

The invention discloses bioactive macroporous hydrogel as well as a preparation method and application thereof. The bioactive macroporous hydrogel comprises hydrogel particles and a bioactive load loaded on the hydrogel particles, the hydrogel particles are obtained by mechanically extruding a hydrogel matrix, and the hydrogel matrix is formed by compounding sodium alginate, agarose and methacrylated hyaluronic acid through hydrogen bond crosslinking and photo-crosslinking; the bioactive load comprises: a) small extracellular vesicles of which the surfaces are modified with macrophage targeted CRV peptides; and b) a PLGA (poly (lactic-co-glycolic acid)) microsphere loaded with a cartilage inducer Kartogenin. The bioactive macroporous hydrogel disclosed by the invention has an internally communicated macroporous network, is beneficial to cell infiltration, blood vessel ingrowth and nutrient substance diffusion, and overcomes the defects that the traditional hydrogel is small in pore size and limits cell behaviors. According to the bioactive macroporous hydrogel disclosed by the invention, dual bioactive loads are adopted to synergistically promote tendon-bone healing, so that the treatment effect is better.

The invention discloses a method for efficiently leaching waste battery positive electrode powder by using hydrogel to assist bacteria, belonging to the field of resource recovery technology. The invention utilizes hydrogel to assist bacteria to leach valuable metal elements from waste LIBs. The bacteria include L. plants and / or L. acidophilus , the hydrogel is prepared by polyvinyl alcohol and agarose. The present invention creatively applies hydrogel in the bioleaching process, effectively increases the leaching percentage of valuable metals in waste LIBs, and avoids the use of inorganic acid at the same time. Compared with the traditional chemical leaching technology, the method of the present invention has obvious advantages in operational safety and effectively avoids the risk of secondary pollution to the environment. By using microorganisms, not only the efficient recovery of metal resources in the battery is achieved, but also the environmental friendliness of the whole process is ensured.

The invention relates to the technical field of heat-resistant materials, in particular to a preparation method of a basalt fiber SiO2 aerogel composite heat-insulating material. The preparation method of the basalt fiber SiO2 aerogel composite thermal insulation material comprises the following steps: S1, preparing chopped basalt fibers into a basalt fiber skeleton preform with a certain mechanical property; s2, preparing SiO2 sol; and S3, putting the fiber skeleton into the prepared SiO2 sol, aging, modifying, and drying in stages to obtain the basalt fiber SiO2 aerogel composite material. The material prepared by the method takes the basalt fibers as a reinforced matrix, the cost of the composite material can be reduced, mass production, popularization and application are facilitated, the basalt fiber porous material is prepared by adopting a method of combining gel casting with freeze drying, and the basalt short fibers are bonded by using a silica sol-agarose composite adhesive system, so that the composite material is prepared. The mechanical property of the composite material is improved.

The invention belongs to the technical field of plant fungus molecular biology detection, and discloses an LAMP primer group, a kit and a detection method for detecting oat smut pathogenic bacteria. A group of LAMP (loop-mediated isothermal amplification) specific primers are designed according to a specific sequence on a whole genome of the oat smut pathogenic bacteria Ustilago hordei, results are judged through a real-time fluorescence quantification method, an agarose gel electrophoresis method and an SYBR Green I fluorescent dye developing method, and the oat smut pathogenic bacteria carried by oat seeds are detected. The LAMP detection method for the oat smut pathogenic bacteria, established by the invention, is strong in specificity, high in sensitivity, high in speed and low in cost, provides a new technical means for detection of the oat smut pathogenic bacteria carried by the oat seeds, and has relatively high practical application value.

The invention discloses a device and method for automatically shearing and recycling agarose gel, and relates to the technical field of agarose gel cutting, and the device and method for automatically shearing and recycling agarose gel comprise an image acquisition unit, a three-axis moving mechanism, a gel cutting device, an electrophoresis apparatus and a control unit; the image acquisition unit comprises a light source assembly and an image acquisition camera corresponding to an irradiation area, the image acquisition camera is used for shooting a dyed electrophoresis image, and the light source assembly is used for obliquely irradiating gel to enable a sample strip to develop color; the control unit comprises an analysis processing unit; the analysis processing unit comprises a deep learning detection module and a data processing module; the control unit cooperates with the image acquisition unit, the three-axis moving mechanism and the gel cutting device, so that a complete automatic process from strip identification and positioning after electrophoresis to gel block cutting, grabbing, releasing and recycling is constructed.

The present application discloses a specific DNA fragment for sex identification of Pelteobagrus ussuriensis and a method for sex identification, specifically relating to the technical field of sex identification of Pelteobagrus ussuriensis. The above-mentioned identification method includes the following steps: Dissect 5 female and 5 male Pelteobagrus ussuriensis of a full-sib family to determine the physiological sex; respectively extract DNA from the muscles of female samples and male samples, construct a paired-end genomic DNA library with an insert fragment size of 300 bp, and perform high-throughput sequencing; based on the GWAS and Fst methods, identify that the sex chromosome of Pelteobagrus ussuriensis is chromosome 8, and then screen for Indels within the sex determination region on chromosome 8; screen a 601-bp male-specific DNA sequence and design and synthesize primers; use PCR amplification and agarose gel electrophoresis detection, and the result without specific bands is female, and the one with a 356-bp band is male. This scheme has the advantages of strong specificity, simple primer composition, and more intuitive identification of the sex of Pelteobagrus ussuriensis.

The invention belongs to the technical field of genetic engineering, and discloses application of FGF8 protein in preparation of an antibacterial agent. Escherichia coli BL21 (DE3) is adopted to express grass carp FGF8a and mouse FGF8b, the grass carp FGF8a and mouse FGF8b recombinant protein is obtained after purification of a Ni < 2 + >-TED agarose gel column, and it is found that the two proteins have the remarkable sterilization effect on gram-negative bacteria and gram-positive bacteria, can be used for preparing broad-spectrum antibacterial agents or antibacterial drugs and have high application value.

The invention provides a recombinant III-type humanized collagen gel based on a macromolecular crowding effect as well as a preparation method and application of the recombinant III-type humanized collagen gel. The method comprises the following steps: firstly, preparing an rhCol III solution system; then preparing a macromolecular crowding bath containing dextran 70, polyethylene glycol 8000 and ascorbic acid; then adding an rhCol III solution into the macromolecular crowding bath to form an rhCol III microgel; and finally, constructing a 3D printing molecular exclusion mold system which takes agarose as a substrate and contains a functional additive and a temperature-sensitive polymer as a temperature-sensitive release and spatial constraint component, so as to realize directional construction of the rhCol III microgel. According to the method disclosed by the invention, second-level physical gelling (lt; 15s) of rhCol is realized, and a physiological microenvironment supporting cell proliferation is constructed. The recombinant III-type humanized collagen gel prepared by the method can be used for preparing collagen microgel balls, collagen bundles, 3D (three-dimensional) bio-printing products and the like, and is wide in application range.

The present invention relates to a method for manufacturing a hydrogel. The method comprises the steps of providing an agarose and providing an aqueous solution of a salt comprising Zn2+, wherein the salt is selected from zinc methanesulfonate, zinc acetate, zinc chloride, or a combination thereof. The method further comprises combining the agarose and the aqueous solution to form a mixture and forming a hydrogel from said mixture. The present invention also relates to a hydrogel, an electrolyte comprising the hydrogel, and an energy storage device comprising the electrolyte.

The present invention discloses a preparation method of Inonotus hispidus polysaccharide IHP-1aa: It is obtained by removing fat with ethanol, extracting with water and precipitating with ethanol, then removing proteins by the Sevage method, separating neutral polysaccharide components through a DEAE-52 cellulose column and a DEAE agarose gel column, and finally purifying with Sephadex G-100. The Inonotus hispidus polysaccharide has uniform purity and clear composition, and is a heteropolysaccharide composed of glucose, galactose, mannose, fucose and methylgalactose, with a molecular weight of 26.9 kDa. In vitro experiments show that it has no adverse effects on normal cells, can significantly stimulate macrophages RAW264.7 to secrete NO, IL-6 and TNF-α, has good immunomodulatory activity, and can be used for the development of immunomodulatory products.

The invention belongs to the technical field of biomedical materials, and particularly relates to a short-acting injectable agarose gel implant. The method comprises the following steps: dissolving agarose in a buffer solution at 90-120 DEG C to obtain a clear and transparent solution; cooling to 40-55 DEG C, adding alkali and a chemical cross-linking agent, and stirring to react for 2-5 minutes; standing and reacting for 4 to 24hrs at 45 to 75 DEG C, and dialyzing and homogenizing to obtain the agarose gel implant. The agarose gel implant has good injection performance and can be accurately injected into a needed skin area, the dosage is accurately controlled, and the comfort level and treatment convenience of a patient are greatly improved. Moreover, the agarose gel implant can be quickly degraded, has the advantages of small side effect, good reversibility, flexible treatment scheme and the like, and can be conveniently used as a short-acting agarose gel implant.

The invention is suitable for the technical field of ion exchange membranes, and provides an antioxidant ion exchange membrane and a preparation method thereof.The antioxidant ion exchange membrane comprises a modified microporous membrane and ion exchange resin, the modified microporous membrane comprises a polytetrafluoroethylene fiber membrane and a coating, and the surface of the polytetrafluoroethylene fiber membrane is coated with the coating. The coating is prepared from the following raw materials: modified agarose, modified polyethylene glycol, modified nano cerium dioxide, nano titanium dioxide and water. According to the antioxidant ion exchange membrane provided by the invention, the modified microporous membrane is manufactured, and the modified microporous membrane comprises a polytetrafluoroethylene fiber membrane and the coating coated on the surface of the polytetrafluoroethylene fiber membrane; according to the coating, the modified agarose, the modified polyethylene glycol and the modified nano cerium dioxide are added and have a synergistic effect, so that on one hand, the oxidation resistance of the ion exchange membrane can be improved, and on the other hand, the mechanical property of the ion exchange membrane can be improved.

The invention belongs to the technical field of cosmetics, and particularly relates to a water-in-oil-in-water three-phase coated microbead as well as a preparation method and application thereof. The water-in-oil-in-water three-phase coated microbead is of a spherical structure and sequentially comprises an outer water phase, an inner oil phase and an inner water phase from outside to inside, the outer water phase comprises sodium alginate, agarose, cyclodextrin, glycerin and other components, the inner oil phase comprises caprylic / capric triglyceride, grape seed oil, olive squalane, tocopherol and other components, and the inner water phase comprises sodium alginate, agarose, cyclodextrin, glycerin and other components. And the inner water phase comprises ampelopsis grossedentata extract, astragalus membranacus root extract and other components. According to the invention, an easily oxidized and unstable active matter is fully and firmly protected by adopting a droplet microfluidic coating technology, so that the active matter is free from external stimulation, active ingredients with different effects play multiple roles, and the composition has good anti-inflammatory, soothing and oil-controlling effects and can be applied to the field of cosmetics.

The invention discloses application of DNA (deoxyribonucleic acid) binding protein Mse7 in removal of host nucleic acid in recombinant protein, a chromatography medium based on the DNA binding protein Mse7 comprises biotin ligase, streptavidin agarose gel and the DNA binding protein Mse7, and the DNA binding protein Mse7 is connected with an Avi-tag label. The method comprises the following steps: co-expressing biotin ligase and DNA (Deoxyribose Nucleic Acid) binding protein in vivo to obtain biotinylated Mse7 protein; purifying and concentrating; and finally, incubating and combining the Mse7 with streptavidin agarose gel to form a new chromatographic filler. The DNA binding protein Mse7 serves as a novel chromatography medium to be applied to removal of host nucleic acid in recombinant protein, host DNA can be effectively removed, the purity of a product is improved, and compared with a traditional method, the technology has higher purification efficiency and recovery rate.

The invention discloses an Indel site simultaneously related to the pod number and the single plant yield of soybean, a molecular marker and application of the Indel site and the molecular marker, and belongs to the field of molecular marker-assisted breeding. The Indel site is located at 19bp, 855bp and 388bp of a soybean No.6 chromosome, and the polymorphism is an amplified fragment difference of 89bp. The detection method comprises the following steps: extracting a soybean genome DNA to be identified and carrying out PCR amplification by taking the soybean genome DNA as a template; detecting the size of a PCR amplification product fragment by 1% agarose gel electrophoresis; when the fragment of the amplification product is a small fragment, the soybean to be identified is judged to be a soybean line with multiple pods and high yield, and when the fragment of the amplification product is a large fragment, the soybean to be identified is judged to be a soybean line with small pods and low yield. According to the method, the detection and breeding cost is reduced, the cultivation effect of the soybean high-per-unit-yield variety is improved, the progress of soybean yield improvement is accelerated, and the soybean breeding time is shortened.