283 results about "Agarose" patented technology

The invention belongs to the technical field of plant fungus molecular biology detection, and discloses an LAMP primer group, a kit and a detection method for detecting oat smut pathogenic bacteria. A group of LAMP (loop-mediated isothermal amplification) specific primers are designed according to a specific sequence on a whole genome of the oat smut pathogenic bacteria Ustilago hordei, results are judged through a real-time fluorescence quantification method, an agarose gel electrophoresis method and an SYBR Green I fluorescent dye developing method, and the oat smut pathogenic bacteria carried by oat seeds are detected. The LAMP detection method for the oat smut pathogenic bacteria, established by the invention, is strong in specificity, high in sensitivity, high in speed and low in cost, provides a new technical means for detection of the oat smut pathogenic bacteria carried by the oat seeds, and has relatively high practical application value.

The invention discloses a device and method for automatically shearing and recycling agarose gel, and relates to the technical field of agarose gel cutting, and the device and method for automatically shearing and recycling agarose gel comprise an image acquisition unit, a three-axis moving mechanism, a gel cutting device, an electrophoresis apparatus and a control unit; the image acquisition unit comprises a light source assembly and an image acquisition camera corresponding to an irradiation area, the image acquisition camera is used for shooting a dyed electrophoresis image, and the light source assembly is used for obliquely irradiating gel to enable a sample strip to develop color; the control unit comprises an analysis processing unit; the analysis processing unit comprises a deep learning detection module and a data processing module; the control unit cooperates with the image acquisition unit, the three-axis moving mechanism and the gel cutting device, so that a complete automatic process from strip identification and positioning after electrophoresis to gel block cutting, grabbing, releasing and recycling is constructed.

The invention discloses light-driven toxin-enriched composite hydrogel as well as a preparation method and application thereof. The composite hydrogel comprises light-driven hydrogel and detection hydrogel, the light-driven hydrogel is agarose hydrogel doped with Au (at) Ag core-shell nano particles; the detection hydrogel is a double strand formed by an okadaic acid aptamer and a complementary sequence cDNA thereof, and a double strand formed by a domoic acid aptamer and a complementary sequence DNAzyme thereof, the hairpin H1 is used for modifying a quenching group BHQ2 at the 3'end, the hairpin H2 is used for modifying a fluorophore Cy3 at the neck part, the hairpin H3 is used for modifying a fluorophore FAM and a quenching group BHQ1 at the two ends respectively, and the metal ion doped agarose hydrogel is used for catalyzing DNAzyme cyclic shearing; and the photo-thermal driving unit is positioned on the detection function unit. According to the present invention, with the composite system of upper layer photo-thermal enrichment and lower layer dual-signal detection, the rapid and high-sensitivity simultaneous detection of the okadaic acid and the domoic acid is achieved through the combination of the photo-thermal transpiration effect and the HCR and DNAzyme cyclic shearing reaction;

The invention is applicable to the technical field of polymer biomedical hydrogel materials, and provides a preparation method and application of multifunctional agarose-based particle composite hydrogel based on liquid-liquid phase separation, and the agarose-based particle composite hydrogel is a product formed by introducing microsphere particles into an agarose hydrogel body matrix; the microsphere particles are formed by a prepolymer and sodium lignin sulfonate through phase separation mediated by a composite behavior based on non-covalent interaction; the prepolymer is obtained by carrying out amine-epoxy reaction on an amino compound and an epoxy compound in water. According to the prepared agarose-based particle composite hydrogel, a microsphere particle structure is formed on the surface of an agarose matrix while positively charged amino groups and sodium lignin sulfonate are introduced, so that the multifunctional agarose-based particle composite hydrogel is prepared.

The invention provides an adsorption material and a preparation method and application thereof, the adsorption material comprises a carrier and an endotoxin affinity binding ligand, the endotoxin affinity binding ligand comprises a recombinant C factor protein, and the carrier and the recombinant C factor protein are connected through a spacer arm; wherein the carrier comprises at least one of resin, chitosan, cellulose, agarose and glucan; the spacer arm comprises at least one of modified polyethylene glycol, polyvinyl pyrrolidone, polymethylacrylic acid-2-hydroxyethyl ester, polyvinyl alcohol, polyacrylic acid, hexamethylenediamine, divinyl sulfone and glutaraldehyde, and at least one end of the modified polyethylene glycol is provided with carboxyl, aldehyde or amino. The adsorption material provided by the invention can efficiently adsorb endotoxin, has the characteristics of low toxicity risk and excellent blood compatibility, and can improve the safety of blood purification.

The invention discloses KASP primers for identifying genotypes of chicken green-shell eggs and application of the KASP primers. Compared with a common flow of identifying genotypes through PCR (Polymerase Chain Reaction) and agarose gel electrophoresis, the method for performing KASP genetic typing by utilizing the primers with the sequences as shown in SEQ ID NO: 1-3 provided by the invention has the advantages that the steps of preparing agarose gel and performing electrophoresis are omitted, and the economic cost is reduced; when large-scale detection is carried out, a large amount of manpower resources are saved, and the detection time is shortened; analysis is directly carried out according to an instrument detection result, personal errors are reduced, and the accuracy of a typing result is improved.

The invention discloses a degradable preservative film and a preparation method thereof. The film takes nano chitosan / agarose in a ratio of 1: 1 as a matrix, contains 0.5-5wt% of PVA (Polyvinyl Alcohol), 1-4wt% of protein powder or starch, 0.2-3wt% of tea polyphenol / carvacrol, 5-20wt% of glycerol and optional 5-15wt% of polylactic acid, and is cross-linked with sodium alginate. And carrying out tape casting at 55-65 DEG C, and carrying out gradient drying at 40-50 DEG C, thereby obtaining the product. The film is compact and flexible, the breaking strength is larger than or equal to 69 MPa, the oxygen permeability is low, the inhibition zone on six germs is enlarged by 3-4 mm, 98% of natural soil is degraded 20 days later, and no toxic smoke is generated during incineration. The biodegradable film is solvent-free in preparation, can be produced by existing equipment, is lower than a traditional biodegradable film in cost, can be in direct contact with food, remarkably prolongs the fresh-keeping period of fruits and vegetables, replaces PE and PVC, and solves white pollution.

The invention relates to porous ceramic for a high-air-permeability semiconductor device as well as a preparation method and application of the porous ceramic, raw materials comprise 35-55 wt% of alumina powder and 10-55 wt% of a polymer microsphere pore-forming agent, and the polymer microsphere pore-forming agent comprises one or more of agarose, polylactic acid, polyacrylamide and organic silicon resin. The porous ceramic for the high-air-permeability semiconductor device, which is obtained by the preparation method provided by the invention, has relatively high porosity and air permeability, and also has relatively high mechanical strength and dielectric strength. According to the present invention, the gas volume flow rate is applied to the internal pore size characterization, and the high porosity and the high gas permeability can be achieved by accurately measuring the gas flow transmittance, accurately characterizing the pore size and the opening degree in the porous ceramic, and adjusting the particle size and the addition amount of the polymer microsphere pore forming agent; through the preparation process, the porosity and mechanical strength of the porous ceramic for the high-air-permeability semiconductor device can be balanced, meanwhile, high air permeability and high compressive strength are obtained, and the porous ceramic is particularly suitable for electrostatic chucks of different manufacture procedures.

The invention discloses a method for cultivating triploid kelp sporophytes and identifying ploidy of induced protonema, and belongs to the field of kelp breeding. The method is based on a capillary glass needle separation technology for inducing filamentous somatic cells by kelp and a molecular biological technology for specific marking of sex of kelp gametophytes. The sex specific marker of the kelp gametophyte is used for carrying out PCR (Polymerase Chain Reaction) amplification and 1.5% agarose gel electrophoresis detection on the separated induction protonema single-cell cloning line, so that the genetic sex of the kelp induction protonema can be accurately identified; and the ploidy of the induced protonema of a part of varieties (lines) is directly determined from the molecular level. The method for synchronously detecting the genetic sex and ploidy of the induced protonema breaks through the bottlenecks of chromosome counting and flow cytometry operation, can be completed only through one-time PCR amplification, and is simple, convenient, rapid, short in time consumption, capable of completing detection of a plurality of samples at the same time and low in cost.

The present application belongs to the field of molecular genetics, and particularly relates to an ethylene response transcription factor HvERF62 for regulating the moisture tolerance of barley and application thereof in molecular marker assisted selection breeding. A site significantly affecting the moisture tolerance of barley is identified through whole genome association analysis, and a candidate gene is predicted, and further, the moisture tolerance function of the candidate gene HvERF62 is verified by using the CRISPR-Cas9 gene editing method. The function of HvERF62 for regulating the moisture tolerance of barley is determined by using the gene editing technology, and an InDel molecular marker for detecting the moisture tolerance of barley is developed. The molecular marker of the present application is detected by using 3% agarose gel, is stable, simple in method, and can be used for screening the moisture tolerance of barley at an early stage, and has a strong application value in the breeding of the moisture tolerance of barley.

The utility model belongs to the technical field of sepharose gel preparation, and particularly relates to a temperature measuring and blending device for sepharose gel preparation, which comprises an oscillating and blending device, the oscillating and blending device comprises a vibrating plate capable of vibrating, a fixing device for fixing a preparation container is clamped on the vibrating plate, and a temperature measuring device is arranged on the vibrating plate. The fixing device is fixedly connected with a temperature measuring plate for placing a preparation container, and a thermochromic film is arranged on the upper surface of the temperature measuring plate. Compared with the prior art, by arranging the fixing device and the temperature measuring plate on the fixing device, the technical problems that in the prior art, an agarose solution needs to be shaken up manually, and the agarose solution is possibly polluted when a traditional temperature measuring device is used are solved.

The application provides a kind of construction and application for sesbania sesban DNA fingerprint, belong to sesbania molecular marker technical field, the application is based on sesbania genome sequencing, first develops and screens out 8 pairs of SSR primers with sequence specificity and rich polymorphism, by analyzing the agarose gel electrophoresis result of PCR amplification product of SSR primer in different varieties, the assignment of different size, type of PCR amplification product is carried out, to form the DNA fingerprint of each sesbania, the application has the characteristics of easy operation, good repeatability, simple data processing, low cost and the like, and lays a foundation for the classification, identification and utilization of sesbania germplasm resources.

The application belongs to the field of fish development, and particularly relates to a molecular marker for identifying Nibea albiflora sperm-induced Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring, a primer set thereof, a kit and application. The primer set for identifying the molecular marker of the Nibea albiflora sperm-induced Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring comprises primers F and R, the nucleotide sequence of the primer F is shown as SEQ ID NO. 1, and the nucleotide sequence of the primer R is shown as SEQ ID NO. 2. Based on genomic data analysis, the application develops the molecular marker F / R which can effectively identify the Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring in the juvenile stage. The method comprises extracting genomic DNA of parents and offspring, PCR amplification and agarose gel electrophoresis detection, and finally distinguishing the gynogenesis individuals and hybrid individuals by whether the Nibea albiflora parent marker is contained in the electrophoresis band.

A primer pair, method, and use for rapidly identifying newborn nude mice. On the basis that a deletion mutation occurs in the base G at position 337 of a coding region of the Foxn1 gene in nude mice, a primer pair introducing an enzyme digestion site is designed according to sequence characteristics. Using the genome of a mouse to be tested as a template, the Foxn1 gene is amplified according to a PCR-RFLP method. The PCR product is then digested with a SmaI restriction endonuclease, and the digested product is genotyped by agarose gel electrophoresis. In addition, a reaction system that can be combined with lateral flow technology is screened, thereby achieving instant detection of the newborn nude mice. The method has the characteristics of simple operation, short time consumption, low cost, instant detection, etc.

This invention discloses the application of green tea acidic polysaccharide GTPS in improving metabolic syndrome, aiming to provide a new use for green tea acidic polysaccharide GTPS. The extraction process involves first grinding green tea into powder, boiling, filtering, deproteinizing using the Seval method, concentrating, adding ethanol, allowing it to stand overnight, centrifuging to collect the precipitate, dissolving it, removing the ethanol, and freeze-drying to obtain GTPSs. The GTPSs are then passed through a DEAE-agarose FF column, eluted with distilled water and 0.2, 0.5, and 1.0 mol / L sodium chloride, and the fractions are collected. The fraction eluted with 0.2 mol / L sodium chloride is dialyzed to desalt, yielding green tea polysaccharide GTPS. Applying this to the improvement of metabolic syndrome provides a solid foundation for revealing the key active components in green tea that promote metabolism and paves the way for the future utilization of tea polysaccharides in the pharmaceutical and food industries, offering a promising solution for the integration of tea resources and the application of natural products.

This invention provides a method and kit for enhancing the yield and quality of gene synthesis products, comprising the following steps: providing a total primer mixture, which includes multiple oligonucleotide single strands encoding a target gene sequence / encoding an operational sequence and sterile water; using the primer mixture as a template, performing the first... PCR The reaction yielded the primary spliced ​​product; a second reaction was then performed using start and end primers. PCR The reaction yielded the crude gene synthesis product; nested primers were then used for a third reaction. PCR The reaction yields a nested structure. PCR Products; nested PCR The product was subjected to agarose gel electrophoresis, and the target band was excised and recovered. This application improves upon traditional gene synthesis methods by adding operation sequences to both ends of the synthesized gene sequence and utilizing highly specific nested primers for nested gene synthesis. PCR It can synthesize more than 2000bp in one go. DNA Double-stranded sequences significantly improve the yield and quality of gene synthesis products.

The application discloses a kind of molecular markers for identifying genetic sex of blue fish and its application, belong to the field of aquatic animal genetics and breeding.The molecular marker includes nucleotide sequence such as the common band of blue fish female and male fish shown in SEQ ID NO:1 and nucleotide sequence such as the unique band of blue fish male shown in SEQ ID NO:2, and the identification method comprises: taking the DNA of the blue fish sample to be identified as template for PCR amplification, after the band of PCR product is determined by agarose electrophoresis, capillary electrophoresis is carried out, if signal appears at 152bp and 168bp simultaneously, then it is male;If only signal appears at 168bp, then it is female.The molecular marker of the application can accurately obtain the genetic sex of different blue fish populations, has the advantages such as simple, fast, efficient, not affected by early undifferentiated gonad of blue fish, etc., is used for blue fish marker assisted breeding, which greatly saves the cost of aquaculture and improves the breeding efficiency.

The application discloses a preparation method of a nanogel preparation for preventing and treating tobacco diseases, and specifically comprises the following steps: (1) preparing bacillus licheniformis bacterial liquid; (2) preparing nanometer selenium precipitate; (3) preparing purified nanometer selenium particles; (4) preparing streptomycin-nanometer selenium solid; (5) preparing nanometer titanium dioxide photo-semiconductor sol; and (6) mixing low-melting agarose powder, the streptomycin-nanometer selenium solid and the nanometer titanium dioxide photo-semiconductor sol, centrifuging, washing, and drying to obtain the nanogel preparation. The nanogel preparation has significant bacteriostatic efficacy, can effectively prevent and treat tobacco diseases (especially tobacco bacterial wilt and tobacco wild fire disease), has a promoting effect on tobacco growth, is simple to make, has strong adhesion, and does not pollute the soil environment.

The invention discloses a dCAPS molecular marker primer linked with a brassica napus saline-alkaline tolerance gene BnaC03.RBGB3 and application of the dCAPS molecular marker primer. The primer comprises a forward primer and a reverse primer, and through PCR amplification and specific restriction enzyme digestion treatment, two genotypes of high resistance and high sensitivity of brassica napus saline-alkaline tolerance can be distinguished on agarose gel electrophoresis; the high-resistance genotype is represented by two electrophoretic bands (195bp and 23bp), and the high-sensitivity genotype is a band which cannot be digested by enzyme. The method disclosed by the invention is simple and convenient to operate and reliable in result, can be used for rapidly screening and identifying the saline-alkaline-tolerant variety of the brassica napus, and provides a powerful tool for breeding the saline-alkaline-tolerant variety of the brassica napus. By implementing the technology, not only is the breeding efficiency improved, but also a new thought and strategy are provided for molecular marker-assisted breeding of saline-alkaline tolerance of crops, and development and application of a saline-alkaline tolerance breeding technology of brassica napus and even other crops are promoted.

In one aspect, the disclosure relates to multilayer composite films comprising a first biopolymer such as agarose, and a second biopolymer such as shellac. In the films, one or both layers may further comprise a biodegradable plasticizer. In a further aspect, the films can be flat or can be thermoformed to have a three-dimensional shape. The films are biodegradable and have very low permeability to water, gases, and other contaminants. Also disclosed are methods of making the films and consumer product packaging materials comprising the films.

This invention discloses a method to avoid the influence of cations on carbon nanotube fluorescent sensors, and a gel-based dopamine sensor based on Al-(AT)15-SWCNTs complex and its application. The method to avoid the influence of cations on carbon nanotube fluorescent sensors first involves preparing a single-stranded DNA sequence (AT). 15 Modified single-walled carbon nanotube solution ((AT) 15 -SWCNTs); then (AT) 15 The SWCNTs solution and agarose gel were mixed at a 1:1 volume ratio and allowed to settle at (AT). 15 -SWCNTs solidify to obtain a gel-based dopamine sensor; the gel-based dopamine sensor is placed in an Al(NO₃)₃ solution for at least one hour, and then washed by immersing it in an HCl solution with pH ≤ 3.7 to remove residual free Al from the gel. 3+ The ions ultimately yield Al−(AT). 15 A gel-based dopamine sensor for ssDNA-SWCNT complexes. Trivalent aluminum ions pre-occupy the interaction sites of phosphate groups to prevent further interactions between other cations and phosphate groups. Agarose gel-based ssDNA-SWCNTs avoid cation-induced aggregation.

The utility model discloses a soluble slow-release acupuncture needle, which relates to the field of medical instruments and comprises a needle handle and a needle body, a clamping mechanism is arranged at the bottom of the needle handle, and the root of the needle body is inserted into the bottom of the needle handle and is fixed by the clamping mechanism; the needle body comprises a fish glue layer, a net-shaped medicine storage layer and an agarose layer, the fish glue layer, the net-shaped medicine storage layer and the agarose layer are sequentially arranged from outside to inside, a cavity is formed in the agarose layer, and the cavity is of a centipede-shaped structure; step-by-step release of the medicine is achieved, and the medicine and physical stimulation of the needle body jointly play a role; the dual curative effects of needle and medicine combination are achieved in the treatment process, and the treatment effect and the patient experience are greatly improved; based on the reusable design of the needle handle, the economical efficiency and practicability of the acupuncture needle are further optimized; the problems that in the traditional acupuncture treatment process, medicine release is not uniform, and the medicine effect maintaining time is insufficient are effectively solved, and the more accurate and continuous treatment effect is provided for patients.

The invention relates to a pharmaceutical composition for repairing oral gingivitis and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises a lactobacillus salivarius fermentation extract, a herba violae extract, golden cicada nymph extract peptide, guava polyphenol and resveratrol; the lactobacillus salivarius fermentation extract is obtained by carrying out aerobic fermentation and anaerobic fermentation on lactobacillus salivarius to obtain a fermentation product, and purifying and extracting the fermentation product through an XAD7HP macroporous adsorption resin column; the philippine violet herb extract is obtained by performing enzymolysis on whole philippine violet herb through pectinase and cellulase and then performing enzymolysis through compound protease to obtain zymolyte, and purifying and extracting the zymolyte through a compound adsorption column. The golden cicada nymph extracted peptide is obtained by purifying and extracting 5kDa zymolyte obtained by performing step-by-step enzymolysis on golden cicada nymphs through papain and trypsin through a Q agarose gel FF adsorption column. All the components are matched for use according to a certain proportion, so that the antibacterial coating is capable of precisely resisting bacteria, has efficient anti-inflammatory and anti-oxidation properties, and is safe and reliable.

The invention relates to a drug-resistant gene targeted enrichment method using dCas9 and application of the drug-resistant gene targeted enrichment method, and belongs to the technical field of nucleic acid enrichment. The drug-resistant gene targeted enrichment method comprises a CRISPR-dCas9 system for targeting a target gene and a target capture magnetic bead matched with the CRISPR-dCas9 system; the CRISPR-dCas9 system comprises a target DNA (Deoxyribose Nucleic Acid), a dCas9 protein and a target nucleic acid targeted sgRNA (Single Guide Ribonucleic Acid), and the targeted capture magnetic bead is a His tag protein purified agarose magnetic bead. According to the method, a CRISPR-dCas9 system and His tag protein are integrated to purify agarose magnetic beads, and dCas9 protein with a His tag is combined with the magnetic beads to construct a novel enrichment scheme; his magnetic beads are combined with dCas9 with a His tag under the condition that protein denaturation is not achieved, and drug-resistant gene targeted enrichment is achieved; the whole scheme is easy to operate, mild in condition, low in cost and suitable for common laboratories and on-site rapid detection to improve sensitivity. According to the method, the target gene is highly specifically recognized through sgRNA, magnetic bead capture is combined, and the method has the advantages of being efficient, specific and easy to operate and has application value in the fields of drug-resistant gene screening and diagnosis, third-generation sequencing and the like.

The application provides a composite implant containing different molecular weight agarose and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The composite implant comprises two or more than two combinations of high molecular weight medical grade agarose, medium-high molecular weight medical grade agarose, medium molecular weight medical grade agarose and low molecular weight medical grade agarose. The application combines medical grade agarose with different molecular weights, so that the composite material has good injectability, moderate gel strength and controllable degradation performance. The prepared composite implant containing different molecular weight agarose has good antioxidant and anti-wrinkle effects, and has a wide application prospect in the field of skin anti-aging.

This invention relates to molecular marker 1769 and its application in the identification of sex-reversed pseudomale individuals in the Northeast Forest Frog (Rana davidii), and pertains to the field of molecular markers. To address the current difficulty in identifying sex-reversed pseudomale individuals in the Northeast Forest Frog, this invention extracts the genome from the muscle tissue of the left hind limb of the Northeast Forest Frog. Using the extracted genomic DNA as a template, PCR amplification is performed using primer pairs containing the sequences shown in SEQ ID NO:1 and SEQ ID NO:2. The PCR amplification products are detected by electrophoresis on a 3% agarose gel. Phenotypic male individuals exhibiting the 1769 bp band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are genetically male Northeast Forest Frogs, i.e., true males. Phenotypic male individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are pseudomale individuals who have undergone sex reversal from genetically female to phenotypically male. Phenotypic female individuals lacking the band corresponding to the nucleotide sequence shown in SEQ ID NO:3 are female individuals.

The invention discloses a high-wettability and strong-adhesion cross-linking enhanced dust fall material. The dust fall material comprises the following raw materials in parts by mass: 2-5 parts of pullulan, 2-5 parts of agarose, 5-15 parts of sodium hydroxide, 5-15 parts of chloroacetic acid, 5-15 parts of ethylene glycol diglycidyl ether, 3-11 parts of malic acid, 5-10 parts of a water-retaining agent, 5-10 parts of diluted hydrochloric acid and 1000-2500 parts of distilled water. The dust falling material has high wettability and strong adhesion, and can effectively settle coal dust in air; meanwhile, a coal dust consolidation layer formed after the dust falling material is sprayed is high in strength, reentrainment of dust can be effectively prevented, and the technical problems that the dust falling material is poor in dust falling effect, single in function and the like are solved.

The preparation method of the agar nanogel interface modification spray is prepared from the following raw materials by weight: agarose 35-70 parts, Tween 80 40-50 parts, dimethyl silicone oil 60-150 parts, and tetrahydrofuran 50-120 parts. The preparation method comprises the following steps: S1: placing agarose into heated deionized water to obtain an agarose aqueous solution, adding Tween 80 and dimethyl silicone oil to obtain a coarse emulsion; S2: ultrasonic dissolving the coarse emulsion to obtain a fine emulsion, cooling and centrifuging, taking the lower liquid, adding tetrahydrofuran to obtain an agar nanogel mixture; S3: rotary evaporation and drying the mixture to obtain agar nanogel; and S4: adding the gel into ethanol or water to obtain the agar nanogel interface modification spray. The agar nanogel spray formed based on the molecular self-assembly strategy has a hexagonal phase columnar structure, the spray can form a modified layer with excellent light-heat conversion efficiency and water transport performance on the water surface, and can significantly enhance the evaporation efficiency.

The invention belongs to the technical field of protein purification, and particularly relates to a chromatography medium, a preparation method of the chromatography medium and application of the chromatography medium to extraction of alpha-lactalbumin. The chromatography medium provided by the invention takes agarose as a matrix, and the surface of the chromatography medium is modified by a ligand through a glucan spacer arm; wherein the molecular weight of the glucan is 20 kDa to 500 kDa, the ligand comprises propylene diamine, and the density of the ligand in the chromatography medium is 200 [mu] mol / mL to 300 [mu] mol / mL. The invention also provides a preparation method of the material, and the material can be obtained by sequentially carrying out epoxy activation, glucan modification, secondary activation and ligand bonding on the matrix. When the chromatography medium provided by the invention is used for extracting alpha-lactalbumin from whey, the chromatography medium has the advantages of high loading capacity, high loading flow rate and efficient regeneration, and meets the requirements of industrial production.

The invention belongs to the field of identification of small gold moth, and particularly relates to a method for rapidly identifying ecotype of small gold moth. The method comprises the following steps: detecting the genotype of a Chr04.g05558 gene segment in a small gold moth genome, and determining the ecotype of the small gold moth according to the genotype; the Chr04. G05558 gene segment is located among Chr04: 51, 430, 521-51, 430 and 805 of the small gold moth, the Chr04. G05558 gene segment as shown in SEQ ID NO: 2 is a high mountain type small gold moth, and the Chr04. G05558 gene segment as shown in SEQ ID NO: 3 is a low mountain type small gold moth. The method disclosed by the invention can be used for identifying the ecotype of the small gold moth larva, is suitable for trace sample analysis, can directly interpret the result through conventional agarose gel electrophoresis, and can further judge the genotyping result through sequencing.