206results about How to "High infection rate" patented technology

Novel burst-free, sustained release biocompatible and biodegrable microcapsules which can be programmed to release their active core for variable durations ranging from 1-100 days in an aqueous physiological environment. The microcapsules are comprised of a core of polypeptide or other biologically active agent encapsulated in a matrix of poly(lactide/glycolide) copolymer having a molar composition of lactide/glycolide from 90/10 to 40/60, which may contain a pharmaceutically-acceptable adjuvant, as a blend of uncapped free carboxyl end group and end-capped forms ranging to ratios from 100/0 to 1/99.

A system and a method of computer controlled irrigation and fertigation composed of one or more sensors positioned in order to quantify the amount of water and/or nutrients that a plant is consuming. By controlling the fertigation, the plant or a part thereof, has improved yield and quality,

The invention relates to a method for growing blueberry, which comprises soil modification, seedling cultivation, seedling planting and fertilization. In the invention, a soil modifier is applied to soil and the organic materials in the soil modifier increase the content of fatty acids such as acetic acid and propionic acid of the soil, so the rate of mycorrhizal infection, which is capable of promoting the growth of the blueberry, improving blueberry yield, contributing to productivity improvement and soil modification and improving quality and sugar content of fruits, of the blueberry is improved; meanwhile, pine soil and grass carbon are used to regulate the pH value of the soil. In the invention, a rooting medium which contains IAA growth hormone and glycine betaine is used to cultivate the seedlings, so the rooting rate of cuttings and transplanting survival rate are improved, the seedling cultivation time is reduced greatly, and the propagation efficiency is improved obviously. In the invention, a microbial fertilizer, a potassium sulfate type compound fertilizer, ammonium sulfate and diammonium phosphate are used for fertilizing the blueberry, wherein the ammonium sulfate can lower the pH value of the soil, namely the ammonium sulfate provides nutrients for the blueberry for growth and improves the growing environment of the blueberry; and thus, the blueberry yield is more improved effectively.

A surgical anchor is provided with one or more anchor holes distributed around the head of the anchor. Each anchor hole is inclined to allow attachment of one or more sutures to the anchor either before, during or after the anchor is seated in a bone. The upper and lower apertures of each anchor hole are chamfered, and the chamfered areas from the anchor hole to the outside edge shall be polished or somehow smoothed to remove sharp edges and rough areas which may cause friction and abrasion to soft tissue or suture material. The lower surface of the anchor head may be angled to further simplify the task of feeding a surgical needle through the anchor hole after the anchor is seated into the bone.

The invention discloses a method for improving growth and drought-resistant ability of wheat by using arbuscular mycorrhizal fungi to bring the reciprocal symbiosis relation between AM fungi and host plants under drought stress into play reasonably and efficiently. According to the method, the AM fungi having a close symbiotic relation with the wheat are selectively used, and the symbiotic relation between the AM fungi and the wheat is built in a condition control mode to promote the wheat growth and improve drought adaptability. The AM fungi comprise one of glomus monosporum and acaulospora laevis, the two kinds of AM fungi are respectively propagated in an expanding mode in an expanding propagation matrix by using red clovers, and then two AM fungi inoculants and the wheat are cultured together in a symbiotic culture medium. The effective path for profitable symbiosis of the AM fungi and the host plants in adversity conditions is provided, the infection and symbiosis relation with the host plants of the AM fungi is improved in a man-made mode, and the yield of dry crops is improved.

The invention relates to a Beaueria bassaria (Balsamo) Vuillemin bacterial strain GZGY-1-3, a culture method of the bacterial strain, spore powder prepared from the bacterial strain and application of the spore powder in preparing a biocontrol agent, wherein the biocontrol agent can be used for preventing and controlling bemisia tabaci, frankliniella occidentalis, green onion aphidand/or green peach aphid. The biocontrol agent can be used for preventing and controlling insects under a high temperature environment, and is good in prevention and control effect with field control efficiency over 75%.

The invention discloses an arbuscular mycorrhizal (AM) fungal inoculant. The AM fungal inoculant is prepared by the following steps: taking white clover seeds and dwarf lilyturf seeds as the host plants, taking glomus mosseae, glomus reticulatum, glomus etunicatum, or glomus intraradices as the inoculant, carrying out culture under specific nutritional conditions, cutting off the parts above the root of plants in the harvest period, grinding the roots and matrix parts, evenly mixing so as to obtain the AM fungal inoculant. According to the preparation method, white clover and dwarf lilyturf are combined according to a specific ratio, the combination is taken as the carrier for fungal propagation, and the propagation carrier is cultured under specific nutritional conditions so as to produce AM fungal inoculant having the advantages of high infection rate and large spore density. The quality of AM fungal inoculant is the best, when glomus mosseae is taken as the inoculant, the infection rate is the highest, and the spore density is the largest. The AM fungal inoculant can be widely applied to scientific research, ecological control, soil improvement, and the like.

The invention relates to a method for the horizontal transfection of bemisia tabaci of the exogenous endosymbiotic bacteria, which pertains to the field of agricultural biotechnology. The preparation method includes: the purification of the exogenous endosymbiotic bacteria Wolbachia, the collection and fixation of a bemisia tabaci receptor, the horizontal transfection of the exogenous endosymbiotic bacteria Wolbachia to the bemisia tabaci receptor, the collection and fixation of an injection individual and the detection of the endosymbiotic bacteria. The invention is simple and quick, which can successfully transfect the exogenous endosymbiotic bacteria to a bemisia tabaci individual, and a bemisia tabaci offspring has higher infection rate.

The invention relates to methods and kits for assessing the risk, for an individual, of developing an adverse event upon treatment with a therapeutic antibody which is capable of Fab-arm exchange, said method comprising the steps of: a) providing a sample from an individual who is a candidate for treatment with said therapeutic antibody, b) assaying said sample for the presence of circulating IgG4 antibodies that binds an antigen known or suspected to be associated with a causative agent of said adverse event, and c) assessing, on the basis of the outcome of the assay of step b), the risk that the individual will develop said adverse event upon treatment with the therapeutic antibody, wherein the risk of development of said adverse event increases with increased level of said circulating IgG4 antibodies.

The invention provides a preparation method of cordyceps militaris nano powder, comprising the following steps: 1) separating wild cordyceps militaris paecilomyces, and purifying to prepare cordyceps militaris strain liquor; 2) selecting health and live insect pupae, sterilizing by ozone for later use; 3) infecting the insect pupae sterilized in step 2) by the strain liquor prepared by step 1); 4) performing bionic cultivation on infected insect pupae obtained in step 4) to obtain cordyceps militaris cells integrating pupalcells and sporocarps into a whole; and 5) carrying out microwave drying and nano grinding on the obtained cordyceps militaris cells cultured in step 4) to obtain the cordyceps militaris nano powder. The preparation method of the invention has low investment and low energy consumption, the prepared cordyceps militaris nano powder is not polluted by sterilization process, and the fineness of the powder can reach nano level, thus the powder can keep original functionalactivity and is easily absorbed by a human body.

A method and apparatus for reattaching the opposed ends of a member, such as a tendon, ligament or bone, during preparing and healing of the member using a surgical repair device that can be securely attached to the member and then safely guided through tortuous anatomy for reattachment and repair. The repair device further includes structural means so as to secure opposed ends of the member against separation during healing. Devices for aiding in the positioning of the surgical repair device are provided.

The invention discloses a cultivation method of Cordyceps militaris (pupa). The method includes the steps of 1, subjecting properly-aged live silkworms to crymo-anesthesia; 2, pouring pore suspension of Cordyceps militaris into the heads of the silkworms; 3, cultivating the treated and survived silkworms at 4-6 DEG C under the humidity of 65-75% until death and stiffing; 4, embedding the dead, stiff silkworms in sandy soil to cultivate for 6-8 days; 5, cultivating at 17-19 DEG C under the humidity higher than 75%, decreasing the humidity to 60-70% when obvious mycelia grow from the heads and primordium of fruiting bodies differentiates, continuing to cultivate until the length of the fruiting bodies on the heads of the silkworms grow to more than 2cm long, and taking out Cordyceps militaris (pupa) before drying. The method has the advantages that inoculation infection rate, stiffing rate and yield are high; nutrients of the obtained product are approximate to those of natural Cordyceps; the shape of the obtained product is approximate to that of wild Cordyceps; the fruiting bodies are full and golden yellow with uniform size, so that standard packaging is facilitated and added value of products is higher.

The invention provides a herpes simplex virus which is separated from yellow race. The virus has high infection rate on the specificity of the host cells of yellow race, has high reproduction speed; so that can be directly used for infecting and dissolve the tumour cells of yellow race, and can also be used as a carrier to carry gene medicine into the cells of yellow race to conduct gene treatment.

The invention belongs to the field of microbiology and provides a mycorrhizal fungi strain efficiently growing with a rhododendron seedling. The problems of the prior art that the seedling growth is slow, the death rate is high, the leaves are yellow, and the like, during the artificial seedling culturing process for the rhododendron are solved. The preservation number of the mycorrhizal fungi strain provided by the invention is CGMCCNo:6010; the mycorrhizal fungi strain belongs to ascomycetes, chactomium globosum, discellaceae, oidiodendron; the mycorrhizal fungi strain provided by the invention can efficiently grow together with the rhododendron seedling; the rhododendron seedling is inoculated in a seedling stage; the infection rate of mycorrhiza is high; the growth of the seedling is obviously boosted; the survival rate of the seedling is increased; and when the pH of soil is 5.5-7.2, the strain inoculated on the seedling can effectively promote the growth of the seedling.

The invention belongs to the field of botany, and provides a method for the quick mycorhiza formation of azalea aseptic seedlings. By the method, the problems of high death rate, low growth speed, yellowing of laminae and the like of azalea seedlings in the prior art are solved. The method comprises the following steps of: preparing a cultivation medium and a nutrient solution; and transplanting rooted seedlings which are sown or subjected to tissue culture into the cultivation medium which is subjected to aseptic treatment, inoculating at least one bacterial block, and placing the seedlings in a culture chamber for culturing, wherein the cultivation medium consists of grass carbon and yellow sand in a weight ratio of (2-3):1; in the nutrient solution, the concentration of glucose is 0.1 to 0.3 percent; and the bacterial block is selected from any one of CGMCC NO: 6010 or 6031, and the inoculating position of the bacterial block is 0.5 to 1.0 centimeter away from a root system. By themethod, mycorrhiza of the azalea seedlings can be formed quickly and is high in infection rate, and the growth of the seedlings is quick, so the method can be used for scientific study and also can be used for the production of annual seedlings of azalea.

The invention discloses a method for earthing-cultivating silkworm insect grass by simulating an ecological environment. The method comprises the following steps: (1) selecting a strain host; (2) enlarging preparation solution of cultivated strain liquid; (3) preprocessing and postprocessing inoculation; (4) preparing an embedding substrate; and (5) management of earthing-cultivating: inoculatingthe strains optimized in the step (2) in a fluid nutrient medium added with 5-8 wt% of silkworm chrysalis meal, after enlarging the cultivation, inoculating in healthy silkworm larvae in the step (1)for infecting and rigidifying, and embedding infected and rigidified silkworm bodies to be developed in the step (3) in a transparent plastic box filled with the plant ash embedding substrate; after sporophore is grown to be matured, taking out the silkworm bodies from the plant ash embedding substrate, and cleaning up plant ash adhered to the periphery of the silkworm bodies, to obtain the matured silkworm insect grass. The method has the characteristics of low sundry fungus contamination rate, high infection rigidifying rate, high production rate, good stability of the obtained silkworm insect grass, higher yield, low cost, and full and regular appearance of the silkworm insect grass, is suitable for the large-scale production, and has the extensive development prospect.