35 results about "Glomus intraradices" patented technology

The invention relates to an application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation. A primary arbuscular mycorrhizal fungi fungicide is inoculated and propagated separately, and propagule spore density of an arbuscular mycorrhiza of each fungi after the propagation is 30 pieces per gram of dry soil. A float breeding method is adopted to conduct arbuscular mycorrhizal fungi fungicide combinedinoculation to tobacco, mycorrhization tobacco seedlings are obtained, and the mycorrhization tobacco seedlings are directly applied to field production. When being transplanted, the mycorrhization tobacco seedlings are conducted with a two-step inoculation, and the mixed fungicide is mixed by propagated acaulospora mellea, glomus mosseae and glomus intraradices at a proportion of 0.5-1: 1:1. A colony formed by the propagation of the arbuscular mycorrhizal fungus is close to a natural state, and fungus cooperate and play a better role of growth promoting and quality improving. According to the application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation, by means of the two times of inoculations of the arbuscular mycorrhizal fungus, the proportion of arbuscular mycorrhizal fungus and the tobacco in a symbiotic system built during a seedling stage of the tobacco is guaranteed, and the beneficial effects of the symbiotic system to the development of the tobacco seedlings are ensured. The application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation is simple and practical in method and environment friendly.

The invention discloses a method for biologically restoring organic phosphorus pesticide polluted soil. An arbuscular mycorrhizal fungi and plant symbiotic system is used for accelerating the recovery of the organic phosphorus pesticide polluted soil. The method comprises the following steps of: (1) preparing an arbuscular mycorrhizal fungi agent; (2) inoculating the fungi agent to the soil to be restored, and planting quick growth plants, or transplanting the plants after mycorrhizal seedling culture; and (3) managing the plants to grow 2 to 3 months. The arbuscular mycorrhizal fungi agent comprises Glomus caledonium 90036, Acaulospora mellea ZZ, Glomus mosseae BEG167 and Glomus intraradices BEG 141). The host plant is selected from quick growth plants such as Sudan grass, alfalfa, corn, broomcorn and the like. The method can reduce the pesticide residue in agricultural products, reduce environment transfer to water, atmosphere and the like and reduce harm to human health, and is simple, convenient and environment-friendly.

The invention discloses a complex bacterium preparation for fixing nitrogen, solubilizing phosphorus and potassium and rooting and a preparation method thereof. The preparation is mainly prepared by mixing the following raw materials in parts by weight: 5-20 parts of azotobacter vinelandii, 10-30 parts of bacillus megatherium, 10-30 parts of bacillus mucilaginosus, 5-15 parts of azotobacter chroococcum, 10-15 parts of glomus intraradices and 10-25 parts of glomus mosseae. The preparation method comprises the following steps: respectively carrying out high-density culture on each strain; grafting to a shake flask filled with a culture medium in a volume ratio of 15-30 percent for activated culture; grafting the cultured strains into a fermentation tank for fermentation culture expansion; dehydrating and drying the culture expanded single strains to prepare hypopus microorganism dried powder; and mixing to obtain the complex bacterium preparation for fixing nitrogen, solubilizing phosphorus and potassium and rooting. The complex bacterium preparation is used for industrial crops, fruit trees, food crops and the like, has the advantages of biological property, environmental friendliness, high yield and high quality in comparison with existing products, and cannot pollute soil.

The invention relates to an organic fertilizer increasing trace element contents of crops. The fertilizer includes fruit and vegetable dregs the water content of which is less than 70%, bean dregs, bean meal and a mycorrhizal fungi. The mycorrhizal fungi is one or more selected from a group consisting of glomus mosseae, glomus etunicatum, glomus intraradices, glomus claroideum, glomus spurcum, entrophospora kentinensis, acaulospora morrowiae and scutelospora nigra. The organic fertilizer can be applied for leaf vegetable type, fruit type, cereal type, and tuber type crops, and can obviously increase growth and weights of the crops.

The invention discloses a method for reducing honeysuckle cadmium accumulation and increasing the yield of honeysuckle by means of glomus intraradices. According to the method, glomus intraradices and honeysuckle are used for symbiotic cultivation. Specifically, after glomus intraradices is subjected to propagation cultivation by means of a host plant, a mixture containing host plant root sections, fungus hyphae, mycorrhiza fungal spores and cultivation soil is harvested, namely a glomus intraradices inoculant; then, the glomus intraradices inoculant is inoculated in a culture substrate for honeysuckle cultivation. The method can promote growth of honeysuckle and increase the yield. More importantly, cadmium can be prevented from transferring from the root to the overground portions, and the concentration of cadmium of the overground portions (stems, leaves and flowers) is reduced. A means is provided for safe honeysuckle production in soil polluted by cadmium, and the method plays an important role in honeysuckle production and application. The method is good in effect, free of secondary pollution and low in cost. A new thinking and a new direction are provided for development and utilization of glomus intraradices.

The invention discloses an embedding method of an arbuscular mycorrhizal fungus and a preparation method of a capsule fungus. The invention uses sodium alginate as a basic carrier to spore, mycorrhizal and hyphae of the fungus Glomus intraroote Embedded in gel particles, the complete infection process can be completed when sown in non-sterilized soil, and the infection rate has a certain increase with the extension of the infection time. Chitosan, gelatin, glycerin, rice, peat, coal cinder, and malt root added different nutrients, so that the infection rate of the bacterial agent was different, and the infection rate of rice was the highest, reaching 42.9% after 25 days.

The invention provides a repairing material, a repairing method and application of an ionic rare earth tailing area, and belongs to the technical field of ecological restoration. The invention provides a repairing material for an ionic rare earth tailing area. The repairing material comprises arbuscular mycorrhizal fungi and pioneer plants, wherein the types of the arbuscular mycorrhizal fungi comprise one or more of glomus intraradices, glomus mosseae and Paraglomus occultum. The types of the pioneer plants comprise one or more of paspalum, ramie and awn. The repairing material provided by the invention has the characteristics of high plant restoration success rate, excellent effect, capability of obviously reducing the water and soil loss rate and the like when being applied to restoration of the ionic rare earth tailing area, and has a good vegetation ecological restoration effect on the ionic rare earth waste mining area in the rainy region in the south; the invention is suitable for rapid treatment of rare earth tailing waste land in southern rainy regions.

The invention discloses a method for promoting sprouting of arbuscular mycorrhizal fungi spore and growth of hypha. The method comprises the steps: screening arbuscular mycorrhizal fungi spores, sterilizing the arbuscular mycorrhizal fungi spores, sucking the sterilized spores by utilizing a sterile gun head under the microscope, transferring the spores onto a culture medium in a culture dish, inoculating 50 spores in each culture dish, culturing in a dark box, and culturing the spores for 20 days under the condition of 26 DEG C. The method has beneficial effects that the glomus intraradices spore sprouting and hypha growth are promoted by adopting 50ml/L root secreted liquid of RiT-DNA transgenic carrot root, glucose and an appropriate nitrogen source. The method has the advantages of simple and convenient operation, use of cheap materials and environmental friendliness.

The invention provides a field fast production method of lawn grass strengthening biological fertilizers and relates to the field fast production method of the lawn grass strengthening biological fertilizers. The field fast production method aims at solving the problems that the preparation method of the existing lawn grass strengthening biological fertilizers is complicated, the time consumption is long, the cost is high, the yield is low, and the wide application and popularization is difficult. The method comprises the following steps that matrixes and flowerpots are sterilized; white clover glass seeds are sprouted; Glomus mosseae and Glomus intraradices are inoculated into the matrixes, and the white clover glass seeds are spread; after white clover leaves are grown, the watering is carried out, the culture is carried out for 20 to 30 days, water is not added until the leaves are withered, soil and roots are recovered, and composite microbial inoculums are obtained; formalin is adopted to sterilize field soil, the composite microbial inoculums and the white clover glass seeds are mixed, and the sowing is carried out; and after the white clover glass grows for 20 to 30 days, the water is not added until the glass is withered, the soil and the roots are recovered, the natural air drying is carried out, the soil and the roots are stored in ventilating drying places, and the lawn grass strengthening biological fertilizers are obtained. The field fast production method has the advantages that the method is simple, the speed is high, the cost is low, and the operation is easy. The field fast production method is used in the field of biological fertilizers.

The invention relates to a preparation method of a compound microbial fertilizer for peony root drenching. The method includes steps: adopting candida tropicalis for fermenting sweet potato residues and fruit wastes to obtain a fermented product I; mixing chicken manure, cow dung, biogas digester residues and wheat straws to realize natural fermentation to obtain a fermented product II; proportionally mixing the fermented product I and the fermented product II, performing inoculation of geotrichum candidum, bacillus megaterium and bacillus mucilaginosus, and fermenting to obtain a fermented product III; adding appropriate amount of a mixture containing mycorrhizal fungi spores of glomus intraradices and glomus mosseae, root internal and external hyphae and infected plant root segments, fish meal, zinc sulfate and bacillus subtilis HS5B5 bacterial liquid, and well mixing to obtain the compound microbial fertilizer for peony root drenching. According to peony growth nutritional and environmental demands, agricultural by-products are used as raw materials, and the prepared compound microbial fertilizer is capable of protecting peony roots and inhibiting pathogenic microorganisms and provides comprehensive nutrition for peony growth to promote growth and prolong flowering phase.

The invention discloses cold-resistant blueberry plants and a cultivation method thereof. The roots of the cold-resistant blueberry plants are infected with fungi, wherein the fungi are selected from one or more of Glomus mosseae, Glomus intraradices and Glomus etunicatum. Because the blueberry plants to be cultivated are inoculated with the Glomus mosseae or the Glomus intraradices or the Glomus etunicatum, the cold resistance of the blueberry plants to be cultivated is improved; even if the blueberry plants are attacked by low temperature in the cultivation process, the content of hazardous substances in plant bodies can be kept low by generating beneficial substances, and then the good cold resistance of the blueberry plants is achieved.

The invention provides a method for preventing and treating a shallow water system eutrophic lake by virtue of aquatic plants. The method comprises the following steps: (1) with an emergent aquatic plant as a host, inoculating glomus intraradices to a pot culture matrix, and carrying out culturing at 25-30 DEG C for 5-10 days, wherein the mass ratio of zeolite to medical stone to acid-modified argil in the pot culture matrix is 1 to 3 to (0.1-0.3); (2) adding seeds of the emergent aquatic plant into water in a volume ratio of 1 to 2, soaking for 12-24 hours, uniformly scattering the seeds to aculture bed, carrying out culturing at 28-35 DEG C for rooting, covering the culturing bed with a layer of water-immersed silk screen cloth, and transplanting after roots grow to the lengths of 3cm-5cm; (3) fetching adlittoral mud of the eutrophic lake as a soil medium, mixing the adlittoral mud with the pot culture matrix in a mass ratio of (2.5-3) to (1-1.2), carrying out constant-temperature fermentation at 25 DEG C for 24-48 hours, and after the fermentation is finished, transplanting buds of the emergent aquatic plant in the step (2) to the upper part of the mixture; and (4) carrying outculturing by virtue of the culturing bed at 28-35 DEG C for 10-15 days, and externally conveying pots to a shallow water site or an artificial floating island so as to restore an eutrophic water bodyof the shallow water lake when the plant heights are over 8cm, and the infection rates of glomus intraradices at plant roots reach over 95% and the ion concentration of Fe is 4.2mg/L-6.4mg/L throughdetermination.

The invention discloses a rice seed seedling raising method based on combination of glomus intraradices and agrobacterium rhizogenes, relates to a rice seed seedling raising method, and solves the problems that the effect of existing biological fertilizer is only limited to the rice non-flooding period, and the application effect is poor. The method comprises the following steps: accelerating germination of rice seeds, soaking the rice seeds in an agrobacterium rhizogenes agent, filtering, uniformly mixing the rice seeds with the glomus intraradices agent and a rice seedling substrate, and carrying out sowing. According to the seedling raising method, hairy roots generated by agrobacterium rhizogenes induced rice and rice protogenetic root systems can be infected, bacterium and root symbionts can be formed, vitality can be continuously kept after the rice is transplanted to a paddy field, nutrient substances are continuously provided for the rice, growth and development of the rice are promoted, the yield is increased, the blast resistance is enhanced, the application amount of pesticides and chemical fertilizers in the paddy field is reduced, and the ecological agriculture is further developed while the rice production cost is reduced.

The invention belongs to the field of soil improvement, and particularly relates to an active coke-based saline-alkali soil improver and a preparation method thereof. The improver is composed of a porous biological fertilizer and a strain inoculant which are independently packaged, wherein the porous biological fertilizer is obtained by mixing, aging and fermenting an active coke mixture, vinegar residues and chicken manure according to the weight ratio of 1: (0.2-5): (0.2-1); the active coke mixture is obtained by fully mixing active coke screen underflow subjected to homogenization pretreatment and acidification impurity removal with a calcium superphosphate saturated solution and a silicon fertilizer according to the solid weight ratio of 1: (0.5-5): (0.2-1) by utilizing alternating-frequency magnetic coupling ultrasonic grading mechanical stirring; and the strain fungicide is composed of mycorrhizal fungi and a water-retaining agent according to the weight ratio of (0.1-1): (1-5), the mycorrhizal fungi are obtained by compounding glomus mosseae, glomus intraradices and glomus versiforme according to the weight ratio of 1: (0.2-2): (0.5-5) and propagating in local soil, and the inoculated product is a rhizosphere mixture composed of spores, hyphae and mycorrhizal fragments.

The invention relates to a microbial preparation and application thereof, in particular to a microbial preparation for greenhouse planting and an application thereof. The method aims at solving the problems that in the greenhouse planting process, a chloroplast base grain structure is damaged, so that the crop yield is reduced, and the quality becomes poor. The microbial preparation disclosed by the invention is composed of glomus mosseae HDSF1 and glomus intritus HDSF5. The microorganism is applied to greenhouse planting. The microbial preparation is applied to greenhouse planting, particularly to greenhouse soil subjected to pesticide damage and salinization stress, damage of the pesticide damage and salinization to the base grain structure of chloroplast is relieved, normal production of crops is guaranteed, and by means of the microbial preparation, the crops are high in yield and good in quality.

The invention discloses application and method for water saving and yield increasing of dry land wheat through cooperation of nano elemental iron and arbuscular mycorrhizal fungi, and in the wheat planting process, nZVI and AMF are jointly used for treatment, so that the water utilization efficiency and yield of the dry land wheat can be remarkably improved, and glomus intraradices is adopted as the arbuscular mycorrhizal fungi; and propagating the glomus intraradices to obtain glomus intraradices inoculum containing 150-200 spores/10g of dry soil, hyphae inside and outside roots and infected root segments. The method comprises the following steps: firstly, carrying out seed soaking treatment by utilizing a solution of nano elemental iron, then adding a glomus intraradices inoculum or an inactivated glomus intraradices inoculum into a culture medium or field soil, and then sowing the wheat seeds subjected to seed soaking treatment. The symbiotic relationship between the AMF and the dry land wheat is enhanced through the nZVI, the growth of the wheat is promoted by utilizing the synergistic effect of the nZVI and the glomus intraradices inoculum, the water utilization efficiency and the drought adaptability of host plants are improved, and the method is beneficial to coping with the drought and changeable climatic environment.

The invention discloses a method for promoting growth of banana seedlings by inoculating arbuscular mycorrhizal fungi. According to the method, it is found that an arbuscular mycorrhizal fungus mixedstrain (glomus intraradices and glomus manihotis are mixed at a spore ratio of 1:1) has a significant improvement effect on growth and development of banana seedlings as an efficient and safe biological fertilizer and has better ecological economic benefits and environment sustainability effect than other biological fertilizers as a renewable resource for sustainable development through inoculating the arbuscular mycorrhiza fungus mixed strain into banana roots. The arbuscular mycorrhiza fungus mixed strain has a promotion effect on growth of bacteria, fungi, actinomycetes, nitrogen-fixing bacteria and ammonifiers in a culture substrate, and plays an obvious optimization role in metabolic activity of soil microbial communities of the banana seedlings three months after being inoculated, and growth of banana tissue culture seedlings is significantly promoted by a large number of bacterial metabolic activities.