39 results about "Glomus mosseae" patented technology

The invention relates to an application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation. A primary arbuscular mycorrhizal fungi fungicide is inoculated and propagated separately, and propagule spore density of an arbuscular mycorrhiza of each fungi after the propagation is 30 pieces per gram of dry soil. A float breeding method is adopted to conduct arbuscular mycorrhizal fungi fungicide combinedinoculation to tobacco, mycorrhization tobacco seedlings are obtained, and the mycorrhization tobacco seedlings are directly applied to field production. When being transplanted, the mycorrhization tobacco seedlings are conducted with a two-step inoculation, and the mixed fungicide is mixed by propagated acaulospora mellea, glomus mosseae and glomus intraradices at a proportion of 0.5-1: 1:1. A colony formed by the propagation of the arbuscular mycorrhizal fungus is close to a natural state, and fungus cooperate and play a better role of growth promoting and quality improving. According to the application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation, by means of the two times of inoculations of the arbuscular mycorrhizal fungus, the proportion of arbuscular mycorrhizal fungus and the tobacco in a symbiotic system built during a seedling stage of the tobacco is guaranteed, and the beneficial effects of the symbiotic system to the development of the tobacco seedlings are ensured. The application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation is simple and practical in method and environment friendly.

The invention discloses a stress-tolerant growth-promoting composite mycorrhizal fungi inoculant for a pine or fir artificial forest, and a preparation and application methods thereof. The composite mycorrhizal fungi inoculant is compounded from an endomycorrhizal fungi inoculant and an exotrophic mycorrhiza fungi inoculant, wherein the endomycorrhizal fungi inoculant is obtained by culturing Glomus mosseae or Glomus intraradices in pots in a greenhouse; and the exotrophic mycorrhiza fungi inoculant is obtained by fermenting Lactarius hatsudake Tanaka and Scleroderma polyrhizum Pers. in a liquid fermentation medium. In the forestation process, the seedling root system is subjected to slurry dipping treatment or embedded in pine or fir forest soil, thereby promoting rootage and growth of pines or cedars, improving the tree vigor, enhancing the stress tolerance, increasing the absorptivity of P, and preventing the pine leaf cast, fir anthracnose, pine seedling blight and cataplexis.

The invention discloses an arbuscular mycorrhizal fungus strain and application thereof to resisting root-knot nematode. The strain is arbuscular mycorrhizal fungus strain Glomus mosseae 96022CGMCC No.3451. The arbuscular mycorrhizal fungus strain Glomus mosseae 96022 can be applied to preventing and curing diseases caused by plant root-knot nematode. Proved by pot experiment results, the inoculation of the arbuscular mycorrhizal fungus strain Glomus mosseae can obviously inhibit damages to cucumber roots of the root-knot nematode so as to have a certain preventing and curing effect, and the preventing and curing efficiency reaches 41 percent.

The invention provides a live microbial composition for fermentation treatment of cultivating wastes. The composition is characterized by consisting of bean fiber monad, bacillus stearothermophilus, lactobacillus, methanobacteria, thiobacillus, bacillus licheniformis, bacillus polymyxa, streptomycete, thermoactinomycete, thermomonospora, nitrogen-fixing bacteria, nitrated monad, nitrobacteria, rhizobium, Aspergillus oryzae, yeasts, moulds, humicola, rhizopus and glomus mosseae. In addition, the invention further provides an organic fertilizer generated by virtue of fermentation of the microbial composition as well as an application and a method thereof. In production of the organic fertilizer, cultivating wastes such as animal wastes are objectively treated.

The invention discloses a method for repairing glomus mosseae-contaminated soil through combination of glomus mosseae and a nightshade-rice intercropping system. The method comprises the following steps: propagating to prepare a glomus mosseae inoculant; accelerating germination of the rice and solanum nigrum seeds to obtain rice and solanum nigrum seedlings; transplanting rice and solanum nigrumseedlings into the glomus mosseae-contaminated soil, wherein the intercropping ratio of solanum nigrum to rice is 1: (1-2); and adding the glomus mosseae inoculant into glomus mosseae contaminated soil to form a hyperaccumulator-dry rice-glomus mosseae symbiotic system. By inoculating glomus mosseae, the growth and soil Cd absorption capacity of solanum nigrum can be improved, meanwhile, the content of heavy metal cadmium in rice is remarkably reduced, the effect of repairing Cd-contaminated soil while safe production is achieved is achieved, and the method has the advantages of being efficient, low in cost, easy to operate, free of secondary pollution and the like and has good theoretical and application and popularization value.

The invention provides a repairing material, a repairing method and application of an ionic rare earth tailing area, and belongs to the technical field of ecological restoration. The invention provides a repairing material for an ionic rare earth tailing area. The repairing material comprises arbuscular mycorrhizal fungi and pioneer plants, wherein the types of the arbuscular mycorrhizal fungi comprise one or more of glomus intraradices, glomus mosseae and Paraglomus occultum. The types of the pioneer plants comprise one or more of paspalum, ramie and awn. The repairing material provided by the invention has the characteristics of high plant restoration success rate, excellent effect, capability of obviously reducing the water and soil loss rate and the like when being applied to restoration of the ionic rare earth tailing area, and has a good vegetation ecological restoration effect on the ionic rare earth waste mining area in the rainy region in the south; the invention is suitable for rapid treatment of rare earth tailing waste land in southern rainy regions.

The invention relates to a microbial preparation containing arbuscular mycorrhizal fungi and application thereof, and belongs to the technical field of plant cultivation. According to the application disclosed by the invention, it is found that all of the glomus amarae, the rhizopus intro-rhizosporium, the glomus mosseae and the cyst macrosporium in the arbuscular mycorrhizal fungi can promote the growth of plants under the condition of double pollution of molybdenum and micro-plastics, and the plant height and the grain yield of the plants are effectively improved. Further experiments find that under the same condition, the effect is better than that of applying a single arbuscular mycorrhizal fungus inoculant when the near-phoma globosum, the rhizosporium intraradices and the glomus mosseae or the mixed inoculant of the near-phoma globosum, the glomus mosseae and the cyst macrosporium is applied. Furthermore, after beneficial bacteria and auxiliary materials are added into the mixed bacterial agent, the mixed bacterial agent has a better promoting effect on plant growth under the condition of double pollution of molybdenum and micro-plastics. The microbial preparation provided by the invention can reduce poison of microplastics and molybdenum to plants, so that the plants can normally grow under dual pollution.

The invention provides a preparation method and application of an efficient microbial agent for slope protection, and relates to the technical field of ecological restoration. The preparation method comprises the following steps of mixing a bacillus amyloliquefaciens agent, a trichoderma harzianum agent and a glomus mosseae agent according to a mass ratio of 2: 5: 49 to form mixed bacterial powder. The effects of degrading a straw plate and improving the survival rate of slope protection plants are optimal.

The invention aims to provide a saline-alkali soil improver containing glomus mosseae and an improvement method, aiming at solving the problems existing in improvement of saline-alkali soil by the existing biotechnology. The saline-alkali soil improver is composed of glomus mosseae, iron tailings and organic soil, the ratio of glomus mosseae liquid to the iron tailings to the organic soil is 2 mL:2-2.5 g: 1.5-1.8 g, and the concentration of the glomus mosseae liquid is 1-9 * 107 CFU / ml. According to the simple saline-alkali soil improvement method, the glomus mosseae infects plant roots to form arbuscular mycorrhizal symbionts, the iron tailings are used as an improver, a synergistic effect is achieved on plants planted in saline-alkali soil, and the plant biomass is improved while the purposes of improving the saline-alkali soil and improving the soil availability of the saline-alkali soil are achieved.

The invention aims to solve the problems existing in an existing alkaline soil improvement technique, and provides a saline-alkali soil improvement agent containing composite microbial agents and a preparation method and application of the saline-alkali soil improvement agent containing composite microbial agents. The saline-alkali soil improvement agent disclosed by the invention consists of glomus mosseae, azospirllum brasilense and solid carriers, wherein the mass ratio of total number of effective viable bacteria of the glomus mosseae and the azospirllum brasilense to the solid carriers is1*10<5>-9*10<7> / gram, the ratio of the number of the glomus mosseae to the number of the azospirllum brasilense is 1 to (0.2-5). The saline-alkali soil improvement agent has good effects on plage saline-alkali soil improvement. The application of the saline-alkali soil improvement agent is a method for microorganism ecological improvement of saline-alkali soil, which is simple to operate, low incost and pollution-free.

The invention discloses an active organic insecticide fertilizer for bacteriostasis and desinsectization and a preparation method thereof. The active organic fertilizer comprises 10 to 20 parts of aconite, 5 to 10 parts of ligularia sibirica, 4 to 8 parts of pedicularis verticillata, 10 to 15 parts of radix euphorbiae lantu, 20 to 30 parts of whin, 2 to 3 parts of achnatherum inebrians, 5 to 10 parts of pulsatilla chinensis, 3 to 8 parts of tanacetum vulgare stem, leaf and seed, 50 to 60 parts of spent mushroom compost , 10 to 20 parts of cottonseed meal, and 5 to 10 parts of rice hull. The preparation method comprises the following steps: crushing raw materials; supplementing water; treating by the solid material microwave technology; adding 80 to 120g / T of active biological compound bacterium, and performing composting fermentation to obtain the bacteriostatic active biological organic insecticide fertilizer, wherein the active biological compound bacterium is prepared by directly mixing bacillus mucilaginosus, bacillus megatherium, bacillus subtilis, glomus mosseae and glomus versiforme berch. The organic insecticide fertilizer is the organic insecticide fertilizer containing bacteriostasis and desinsectization components and active organisms and is worthy of wide application.

The invention provides a cultivating method for solanaceous plants. The method comprises the following steps of implanting rooted seedlings obtained through seeds or tissue culture into solid substrates, inoculating Glomus mosseae to the substrates, inoculating pseudomonadaceae to the substrates after the plantules of seeds germinate and are exposed out of the substrates to form seedlings, inoculating pseudomonadaceae to the substrates while the rooted seedlings are implanted to the substrates and when the rooted seedlings obtained through the tissue culture are cultivated, obtaining the seedlings with the fungi after the pseudomonadaceae is inoculated, and cultivating the seedlings to ripe. According to the method, in the cultivating process of the solanaceous plants, the Glomus mosseae and the pseudomonadaceae are inoculated to the seedlings successively, the cultivated solanaceous plants are made to show excellent drought resistance, frost resistance, disease resistance and other resistance performance in a seedling stage, and meanwhile, the grow rate of the seedlings of the solanaceous plants is improved greatly under the synergistic effects of the two symbiotic fungi.

The invention discloses an amino acid compound foliar fertilizer, which is prepared from the following raw materials in parts by weight: 150 to 220 parts of urea, 1170 to 1275 parts of 50 percent plant source enzymolysis compound amino acid concentrated solution, 150 parts of monopotassium phosphate, 20 parts of copper sulfate pentahydrate, 20 parts of zinc sulfate heptahydrate and 15 parts of compound bacteria consisting of glomus mosseae and bacillus thuringiensis. The foliar fertilizer is convenient in raw material taking, simple and convenient in process operation, safe, environment-friendly and pollution-free in preparation process, contains nutrient elements and nutrient coordination substances required in celery growth, improves the celery yield, promotes celery quality improvement, and provides powerful support for green and sustainable development of the celery industry.

The application discloses a method for improving the rhizosphere micro-ecology of tomatoes by using Pseudomonas moraviensis in combination with arbuscular mycorrhizal fungi and application thereof, wherein the strain HY-S70 belongs to Pseudomonas moraviensis and has a preservation number of CGMCC No.32673. Before planting tomatoes, arbuscular mycorrhizal fungi inoculum (preferably Glomus mosseae) is inoculated into soil at an inoculation amount of 30 spores / g inoculum; and 3-5 days after planting the tomatoes, Pseudomonas moraviensis bacterial liquid with a bacterial concentration of 1x10 9 CFU / ml is inoculated. The method provided by the application can activate phosphorus in soil, promote the growth of tomato plants, improve the rhizosphere microbial community structure of the tomato plants and optimize the rhizosphere micro-ecological environment of the tomato plants, and has a wide application prospect.

The invention relates to a method for improving the growth and drought resistance of Atractylodes atractylodes seedlings, which belongs to the field of processing Atractylodes rhizomes. The method adopts Glomus mosesi as the first strain and Glomus tetus as the second strain, and treats The first strain and the second strain are mixed after a unique propagation work, and the obtained arbuscular mycorrhizal fungus propagation mixture is applied to the Atractylodes macrocephala seedling substrate added with dry chicken manure, and the cerium chloride solution is used to Treat Atractylodes macrocephala seeds, and spray Atractylodes atractylodes seedlings with special spraying water, thus overcoming the problems in the prior art that Atractylodes atractylodes is severely degraded after multiple generations of reproduction, resulting in weakened resistance, reduced quality, and high seedling mortality. Further, the growth ability and drought resistance ability of Atractylodes macrocephala seedlings are significantly improved, and seedlings with strong growth ability and drought resistance ability can be provided for Atractylodes rhizome production, thereby expanding the development of Atractylodes rhizome production and expanding the development and utilization of Atractylodes rhizome resources.

The present invention relates to the field of biological bacterial fertilizer and bacterial agent, in particular to a preparation method of solid bacterial agent for rice crops, comprising the following steps: 1) grinding the fertilizer into fine powder; 2) dissolving sodium alginate and polyvinyl alcohol Fully dissolve in water, add CaCO to the resulting mixture 3 ; Then drop into the cross-linking agent to solidify to form gel balls; 3) Wash the gel balls with an acidic solution to obtain a porous gel material; 4) Graft N-isopropylacrylamide on the surface of the porous gel material ; 5) Mixing and culturing the fungus liquid of Glomus moses and the obtained porous gel material to obtain a solid gel bacterial preparation. The present invention uses sodium alginate / polyvinyl alcohol porous gel material as a carrier, and the pore wall in the porous gel material is covered with fertilizer fine powder, and a large amount of Glomus moses mold is adsorbed on the surface of the pore wall, and the material is Biodegradable materials, will not remain in the soil, green and environmentally friendly.

The invention discloses a method for relieving castor salt stress by using melatonin and arbuscular mycorrhizal fungi and application, and belongs to the technical field of plant protection. The arbuscular root fungus is a mixed strain of glomus mosseae enzyme and rhizopus intraradices according to a spore ratio of 1: 1. Experiments prove that damage of stress to ricinus communis can be effectively relieved by using melatonin and arbuscular mycorrhizal fungi, the content of proline, soluble sugar and soluble protein is increased by remarkably improving the activity of antioxidant enzyme, and the net photosynthetic rate, stomatal conductance, intercellular CO2 concentration, transpiration rate and actual photochemical efficiency are remarkably increased, so that the stress resistance of the ricinus communis is improved. The melatonin and the arbuscular mycorrhizal fungi are utilized to effectively relieve the damage of salt stress to the ricinus communis, improve the salt tolerance of plants and solve the key growth problem of the ricinus communis caused by soil salinization, so that the application of the melatonin and the arbuscular mycorrhizal fungi can effectively relieve the damage of the salt stress to the ricinus communis, improve the salt tolerance of the plants and improve the soil salinization resistance of the ricinus communis. In addition, no potential safety hazard and risk of mutation of germplasm genes exist in the treatment process, the safety of prevention and control measures is greatly improved, and the method is a green, safe and environment-friendly method for relieving the harm of salt stress to the castor.

The invention discloses an arbuscular mycorrhizal fungi inoculant for improving growth vigor and yield of pepper in a pepper seedling stage and application of the arbuscular mycorrhizal fungi inoculant, relates to the technical field of agricultural biology, and solves the problem of an AMF inoculant which is optimally combined according to pepper variety characteristics and can stably, efficiently and comprehensively improve the quality and final yield of pepper seedlings. According to the technical scheme, the method is characterized in that three kinds of fungicide, namely, rhizosporium intraradices, phomotheca mosseae and glomus parvicularis, are used for conducting independent inoculation on chilies, the spore concentration of the inoculant of the rhizosporium intraradices is 200 per gram, the spore concentration of the inoculant of the phomotheca mosseae is 370 per gram, and the spore concentration of the inoculant of the glomus parvicularis is 290 per gram; the method has the effects that the infection success rate and stability are improved: the fungicide provides various infection strategies and functional hypha networks, the probability of successfully establishing symbiotic relationships with root systems of different pepper varieties is increased, and the stability of the growth promoting effect is ensured.

The invention belongs to the field of fungi for plants, and particularly relates to a method for improving the content and yield of total flavonoid of clematis plants by using arbuscular mycorrhizal fungi. By inoculating the clematis plants with glomus mosseae under different contents of nitrogen (N) and phosphorus (P), the growth, the physiological and biochemical characteristics and accumulationof total flavonoid of the clematis plants are influenced, and by utilizing a qRT-PCR technology, the expression characteristics of responses of key enzyme genes to different contents of N and P in the synthesis pathway of flavonoid biomass are analyzed, so that influence mechanisms of the fungi on the growth of the clematis plants and accumulation of active ingredients are found, and a basis is provided for the application of the arbuscular mycorrhizal fungi in the cultivation, resource development and utilization and other aspects of medicinal plants.

The invention belongs to the technical field of plant seedling propagation, and discloses a matrix for maple cuttage. The matrix comprises glomus mosseae, trichoderma asperellum, azotobacter chroococcum and bacillus megatherium. When the matrix is adopted for cuttage, cutting slips do not need to be disinfected and do not need to be soaked by a plant conditioning agent, the process is shortened, manpower and material resources are saved, efficiency is improved, and the rooting rate is high.

The invention provides a coal gangue storage yard ecological restoration method based on composite AM fungi and plant communities, and belongs to the technical field of environmental restoration. The ecological restoration method comprises the following steps: (1) pretreating coal gangue to form a plant growth base layer; (2) inoculating the plant growth base layer with a composite AM fungus inoculant; the composite AM fungus fungicide is prepared from glomus intraradices, globomegalospora globosa, glomus mosseae and glomus young ferulae; and (3) sowing plant seeds on the plant growth base layer inoculated with the composite AM fungus inoculant. According to the method, the coal gangue storage yard is repaired through the AM fungus fungicide and the composite plant community, dependence on exogenous soil and chemical fertilizer is reduced, the long-term maintenance cost can be reduced, and the method has good biological safety and good application and popularization value.

The invention belongs to the field of soil improvement, and particularly relates to an active coke-based saline-alkali soil improver and a preparation method thereof. The improver is composed of a porous biological fertilizer and a strain inoculant which are independently packaged, wherein the porous biological fertilizer is obtained by mixing, aging and fermenting an active coke mixture, vinegar residues and chicken manure according to the weight ratio of 1: (0.2-5): (0.2-1); the active coke mixture is obtained by fully mixing active coke screen underflow subjected to homogenization pretreatment and acidification impurity removal with a calcium superphosphate saturated solution and a silicon fertilizer according to the solid weight ratio of 1: (0.5-5): (0.2-1) by utilizing alternating-frequency magnetic coupling ultrasonic grading mechanical stirring; and the strain fungicide is composed of mycorrhizal fungi and a water-retaining agent according to the weight ratio of (0.1-1): (1-5), the mycorrhizal fungi are obtained by compounding glomus mosseae, glomus intraradices and glomus versiforme according to the weight ratio of 1: (0.2-2): (0.5-5) and propagating in local soil, and the inoculated product is a rhizosphere mixture composed of spores, hyphae and mycorrhizal fragments.

The invention relates to a microbial preparation and application thereof, in particular to a microbial preparation for greenhouse planting and an application thereof. The method aims at solving the problems that in the greenhouse planting process, a chloroplast base grain structure is damaged, so that the crop yield is reduced, and the quality becomes poor. The microbial preparation disclosed by the invention is composed of glomus mosseae HDSF1 and glomus intritus HDSF5. The microorganism is applied to greenhouse planting. The microbial preparation is applied to greenhouse planting, particularly to greenhouse soil subjected to pesticide damage and salinization stress, damage of the pesticide damage and salinization to the base grain structure of chloroplast is relieved, normal production of crops is guaranteed, and by means of the microbial preparation, the crops are high in yield and good in quality.

The invention provides a container mycorrhizal hardwood cutting seedling raising method for lycium barbarum, which is characterized in that glomus mosseae and glomus intraradices are used as arbuscular mycorrhizal fungi for lycium barbarum nursery stock breeding, a mycorrhizal agent and clay are mixed to prepare slurry for lycium barbarum hardwood cutting, the method is a novel method combining a mycorrhizal technology and traditional cutting, and the method has the advantages that the mycorrhizal technology and the traditional cutting technology are combined; the rooting rate of the cutting slips can reach 95% or above, and the survival rate (10% or above), disease resistance and growth performance of the Chinese wolfberry cutting seedlings are remarkably improved. According to the method, container mycorrhizal cultivation of lycium barbarum is adopted, the seedling raising period can be shortened, the seedling survival rate is increased, the seedling raising cost is reduced, and the income of seedling farmers is increased.

The application relates to the technical field of rice planting, in particular to a salt-alkali soil rice quality-improving and yield-increasing planting method, which comprises the following steps: S1, soil treatment; and S2, soil conditioner preparation, wherein the raw materials of the soil conditioner comprise river sand, clay, water, a mixed solution and calcium sulfate, and the raw materials of the mixed solution comprise pomelo peels, glomus mosseae, arbuscular mycorrhizal fungi and bacillus licheniformis. In the application, the formula of the soil conditioner is optimized, the addition of the river sand and the clay can increase the porosity of the soil, improve the water drainage and air permeability of the soil, prevent the soil from being hardened, calcium ions in the calcium sulfate can promote the formation of the soil granular structure, further improve the loose structure of the soil, the pomelo peel fermentation liquor in the mixed solution can provide rich organic matters for the soil, improve the soil fertilizer retention capacity, and the yield performance of the salt-alkali soil rice planting is effectively improved.

The invention relates to the technical field of agricultural microorganism and crop cultivation, in particular to a compound mycorrhizal fungus growth promoting agent for dioscorea composita and an agricultural light complementary planting method for dioscorea composita. The compound mycorrhizal fungus growth-promoting agent for the dioscorea composita comprises glomus mosseae, streptomyces azofixing and plant rhizosphere growth-promoting bacteria, and can greatly improve the photosynthesis of the dioscorea composita under a photovoltaic panel and promote the growth and development of the dioscorea composita. By adopting the compound mycorrhizal fungus growth promoting agent, the unoccupied land under a solar photovoltaic panel is utilized, and solar photovoltaic and dioscorea composita planting are combined in a high-ridge close planting mode; the whole planting process is easy to operate and low in cost, and three-dimensional value-added utilization of the land is achieved.

The invention relates to the technical field of remediation of soil-contaminated environments, in particular to a method for enhancing the petroleum-contaminated soil remediation capacity of phytolacca americana. Specifically, an arbuscular mycorrhizal fungal inoculant is inoculated into a phytolacca americana growth substrate to enhance the petroleum-contaminated soil remediation capacity of phytolacca americana. Preferably, the arbuscular mycorrhizal fungal inoculant is a glomus mosseae inoculant. According to the invention, glomus mosseae plays a role in promoting the mineral nutrient absorption, growth and development of phytolacca americana, and enhances the capacity of the phytolacca americana in degrading petroleum pollution in soil. Specifically, after glomus mosseae is inoculated,the biomass of phytolacca americana is increased by 38.55%, and a petroleum degradation rate is increased by 50.00%. By inoculating glomus mosseae, the biomass of phytolacca americana in petroleum-contaminated soil can be remarkably improved, and the petroleum hydrocarbon degradation rate of phytolacca americana in the petroleum-contaminated soil remediation process is remarkably increased. Moreover, the method has the advantages of no negative influence on the environment, no secondary pollution, good remediation effect and low cost.

The invention provides a method for cultivating Solanaceae plants, comprising the following steps: implanting seeds or rooted seedlings obtained by tissue culture into a solid substrate, and inoculating the substrate with Glomus mosei; the germs of the seeds germinate and are exposed outside the substrate to form seedlings Afterwards, inoculate Pseudomonas to matrix; When cultivating the rooted shoots that tissue culture obtains, then inoculate Pseudomonas to matrix simultaneously when implanting matrix; After inoculating Pseudomonas, obtain the seedling of bacterialization; Seedlings are grown to maturity. The present invention proposes to inoculate Glomus mosei and Pseudomonas successively to its seedlings during the cultivation process of Solanaceae plants, The cultivated Solanaceae plants exhibit excellent stress resistance characteristics such as drought resistance, frost resistance, and disease resistance at the seedling stage, and at the same time, under the synergistic effect of the two symbiotic bacteria, the growth rate of the Solanaceae plant seedlings is also greatly improved .

The invention discloses a stress-tolerant growth-promoting composite mycorrhizal fungi inoculant for a pine or fir artificial forest, and a preparation and application methods thereof. The composite mycorrhizal fungi inoculant is compounded from an endomycorrhizal fungi inoculant and an exotrophic mycorrhiza fungi inoculant, wherein the endomycorrhizal fungi inoculant is obtained by culturing Glomus mosseae or Glomus intraradices in pots in a greenhouse; and the exotrophic mycorrhiza fungi inoculant is obtained by fermenting Lactarius hatsudake Tanaka and Scleroderma polyrhizum Pers. in a liquid fermentation medium. In the forestation process, the seedling root system is subjected to slurry dipping treatment or embedded in pine or fir forest soil, thereby promoting rootage and growth of pines or cedars, improving the tree vigor, enhancing the stress tolerance, increasing the absorptivity of P, and preventing the pine leaf cast, fir anthracnose, pine seedling blight and cataplexis.

The invention relates to an application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation. A primary arbuscular mycorrhizal fungi fungicide is inoculated and propagated separately, and propagule spore density of an arbuscular mycorrhiza of each fungi after the propagation is 30 pieces per gram of dry soil. A float breeding method is adopted to conduct arbuscular mycorrhizal fungi fungicide combinedinoculation to tobacco, mycorrhization tobacco seedlings are obtained, and the mycorrhization tobacco seedlings are directly applied to field production. When being transplanted, the mycorrhization tobacco seedlings are conducted with a two-step inoculation, and the mixed fungicide is mixed by propagated acaulospora mellea, glomus mosseae and glomus intraradices at a proportion of 0.5-1: 1:1. A colony formed by the propagation of the arbuscular mycorrhizal fungus is close to a natural state, and fungus cooperate and play a better role of growth promoting and quality improving. According to the application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation, by means of the two times of inoculations of the arbuscular mycorrhizal fungus, the proportion of arbuscular mycorrhizal fungus and the tobacco in a symbiotic system built during a seedling stage of the tobacco is guaranteed, and the beneficial effects of the symbiotic system to the development of the tobacco seedlings are ensured. The application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation is simple and practical in method and environment friendly.